Reference Genes: Essential Criteria for Assessment of the Real-Time PCR Based Virus Detection in Plants Virology
Reference Genes: Essential Criteria for Assessment of the Real-Time PCR Based Virus Detection in Plants Virology
Rongfei Lu1, 2, Zhiyang Liu1, Peng Fang1, Guangyi Chen1, Feng Sun1, Yongjian Fan1, Yijun Zhou1, Tong Zhou1*
ABSTRACT
Real-time quantitative PCR (RT-qPCR) plays an important role in the current field of virus detection and disease dynamics. The validity and the reliability of generated data can be enhanced significantly appropriate measures. As a standard for the relative expression of target genes, the selection of reference genes is crucial. This review describes the history of the RT-qPCR technology, emphasizes the importance of reference genes and enumerates several algorithms to screen reference genes to normalize the RT-qPCR data. Additionally, several possible improvements in the selection of reference genes are discussed.
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