Experimental Evaluation for Graviola Extracts and Eucalyptus Extract’s Capacity to Induce Healing of Hepatocytes in Rabbits Model
Experimental Evaluation for Graviola Extracts and Eucalyptus Extract’s Capacity to Induce Healing of Hepatocytes in Rabbits Model
Heba Saleh Shaheed1, Zainab J. Malik2*, Ghusoon A.A. Alneamah1, Salih T.H.2
ABSTRACT
The objective of studying this research is to discuss the influence of Graviola and eucalyptus extract and their relationship to liver cell regeneration. We used sixteen mature male rabbits by dividing the animals into two groups. As a general anesthesia by using xylazine ketamine and rabbits were anesthetize by using atropine sulfate (1 mg/kg B.W.) inter-muscularly than after 15 min rabbits were anesthetized intramuscular with a mixture consisting of ketamine hydrochloride (40 mg/kg of B.W. and xylazine hydrochloride (20 mg/kg B.W.). After anesthetizing the animals , in the upper part of the abdomen the location of operation is determine, , and the incision surgically made exposing the fourth lobe of the animal. The liver was manipulated with impact forceps, and stitches were applied to the lobe then by using the surgical scissors to cutting the tied lobe away from the suture after applied the three knots were tied, with a 3-0 polyglactin suture the wall of abdominal then re-approximated, and by used a 2-0 polyamide suture the skin was closed. After liver resection, the animals were divide into two equivalent groups; Graviola was given orally to the first group, and eucalyptus extract was given orally for 15 days to the second group. The animals in the both experimental groups that the parameters clinically were including temperature, rate of respiration and heart rate were with in agreeable values postoperatively. However, adhesions of intra-abdomen wall between the liver, various organs, and the wall of abdomen occurred in a varying degree, which were more frequent in the graviola group than in the other treated group. On the 15th day postoperatively histological examinations were performed by using the liver biopsy method. The pathological anatomical outcomes of this study presented that the maturation in the second group and the regeneration in liver were similar. In the graviola group when compared to another treated group the adhesions of the intra-abdominal were in various degrees of valuation and manifestation between the liver, different organs and the wall of abdomen more than frequently. On 15th day postoperatively taking the liver biopsies for histopathological examinations. Histopathologically the results of this research reveal that the liver regenerate is comparable to maturity in the second group.
Keywords | Graviola, Eucalyptus, Hepatoctomy, Rabbits
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November 2024
Vol. 12, Sp. Iss. 1
