Molecular Characterization and Efficacy of Spodoptera Exigua Nuclear Pol Yiiedros'is Virus
Molecular Characterization and Efficacy of Spodoptera Exigua Nuclear Pol Yiiedros'is Virus
El Sayed l , Yasmein A.; Ghareeb2 , A.; El-Dougdoug3, K. A. and Bekheetl , H. K.
ABSTRACT
The beet army worm Spodoptera exigua (Hubner.) is affected by nuclear polyhedrosis virus, the disease specific polyhedral virus particles was found to be associated with the beet army worm (BAW). Inoculation of SeNPV into S.exigua induced characteristic symptoms of slow motion larvae, cuticle showing red color and swilling of the body. Examination of BAW infected extract by light microscope showed polyhedral bodies with concentration (2.3x107PIB/ml). In our study the morphological structures of S.exigua infected with SeNPV (EG) as well as healthy one were examined by SEM. It was showed complete destruction of external larval body, reduction in each abdominal segment size, pro-legs and thoracic legs reduce in size and appear short and stumpy also the oval shape spiracle converted to circular shape with rupture and disrupted inside tissue compared to healthy one. Healthy and infected S.exigua midgut epithelial cells histological structure was checked by using light microscope. It was found that the midgut epithelial cells were the first targets of the virus infection. Columnar cells, goblet cells and regenerated cells loose their identity and become disorganized also breaking down of peritrophie membrane and basement membrane and scatter of viral polyhedra and small empty vacuoles were observed.
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