Obtaining and Use of the Recombinant Bovine Pregnancy-Associated Glycoprotein 1
Obtaining and Use of the Recombinant Bovine Pregnancy-Associated Glycoprotein 1
Anara Ryskeldina, Indira Iskakova, Nurgul Sarina , Alexander Shevtsov , Laura Syzdykova, Alexander Shustov, Yerlan Ramankulov, Marat Kuibagarov*
ABSTRACT
A convenient biochemical marker of cow pregnancy is the bovine pregnancy-associated glycoprotein 1 (boPAG1) antigen. The aim of this work was to produce a recombinant boPAG1 antigen and obtain monoclonal antibodies (mAbs) against boPAG1. We have obtained the boPAG1 cDNA and are reporting its nucleotide sequence. Bacterial expression of a portion of the natural gene encoding a mature form of boPAG1 failed. But a fusion protein made up of E. coli thioredoxin and boPAG1 was efficiently expressed in E. coli. Using the recombinant protein as an antigen, mAbs were generated. One mAb reacted with a protein present in extracts from cow placenta. The described results are useful for creating immunological reagents to detect pregnancy in cows, which is of great practical importance.
Keywords | Bovine pregnancy-associated glycoprotein 1, Fusion protein, Recombinant antigen, Monoclonal antibodies, Placental antigens.
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