Effect of Cysteine Supplementation in Maturation Medium on Bovine Embryos Development
Effect of Cysteine Supplementation in Maturation Medium on Bovine Embryos Development
Chalothon Amporn1, Somchit Guntaprom1*, Sarawut Duongmawong1, Wilasinee Srisanyong1, Sirikanda Thanasuwan1, Phalita Koonnadilokpot1, Dechawut Bunyaluk2, Juggrid Jugsumrit3, Jakrit Yaeram4, Chompunut Lumsangkul5
ABSTRACT
This research study investigated cysteine supplements and their effects upon in vitro maturation (IVM) media. Experimentally, cumulus-oocyte complexes (COCs) which were obtained from bovine ovaries underwent culturing for a period of 22 h in TCM-199 containing an additional 10% fetal bovine serum, streptomycin (100 μg/ml), penicillin (100 U/ml), 25 μg/ml FSH, 2 IU/ml hCG, 0.2 mM sodium pyruvate, 1 μg/ml 17-β-Estradiol, and either 0, 100, or 200 μM/ml cysteine where 0 served as the control. IVF-TALP medium was used to fertilize the mature oocytes which subsequently underwent culturing in a simple culture medium (KSOM). The results showed that percentage of matured oocytes, fertilized oocytes, 8-16 cells developments, blastocyst in 100 µM cysteine group (73.00%, 54.79%, 30.14%, and 17.81%, respectively) were higher than the control group (48.00%, 37.50%, 18.75%, and 10.42%, respectively) and 200 µM cysteine (respectively, 52.00%, 38.46%, 19.23%, and 9.62%) at a significance level of 0.05, but the difference between the 200 µM cysteine group and the control was not significant (p>0.05). The findings confirm that adding 100 µM cysteine to IVM media significantly increased the percentage of matured oocytes, fertilized oocytes, 8-16 cells developments and blastocyst stage.
Keywords | Bovine, Embryo, Cysteine, In vitro maturation, In vitro production
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February 2023
Vol. 11, Iss. 2, Pages 189-354
