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The Effect of miR-1468 on Growth Hormone in Pituitary Cells of Yanbian Yellow Cattle

The Effect of miR-1468 on Growth Hormone in Pituitary Cells of Yanbian Yellow Cattle

Tai-hua Jin1, An-gang Lou2,Jiu-xiu Ji1, Cheng-dou Cui2, Long-zheng Yu2 andLi-zeng Guan1*

1College of Agriculture and Forestry Science, Linyi University, Linyi 276005, China
2 Agriculture College, Yanbian University, Yanji 133000, China
 
Tai-hua Jin and An-gang Lou contributed equally to this work.

*      Corresponding author: guanlizeng@163.com

ABSTRACT

At present, the relationship between miRNAs and GH has been reported. However, the effect of miR-1468 on growth hormone (GH) is unknown. The objective of this study was to evaluate effects of miR-1468 on GH in pituitary cells of Yanbian yellow cattle in this study. Real-time quantitative PCR (qPCR), Western blot, Targetscan and RNAhybrid analysis software and Dual-Luciferase reporter gene system was used to analyze the regulation mechanism between miR-1468 and GH. The results showed that the GH mRNA and protein level in pituitary cells of Yanbian yellow cattle could be significantly decreased by adding miR-1468 mimics (P<0.01), while these were significantly increased by adding miR-1468 inhibitor (P<0.05). The results of bioinformatics analysis and double luciferase reporter gene system validation showed that miR-1468 targeted 3’UTR of cAMP responsive element binding protein 1 (CREB1). miR-1468 mimics could significantly inhibit the CREB1 mRNA and protein level (P<0.01), whereas miR-1468 inhibitor could significantly increase the CREB1 mRNA and protein level (P<0.01). This study demonstrated that miR-1468 could regulate GH in pituitary cells by regulating the expression of CREB1 gene. The results of this study will provide a theoretical basis for studying the mechanism of growth and development of animals regulated by miRNAs. 
 

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Pakistan Journal of Zoology

August

Vol. 53, Iss. 4, Pages 1201-1601

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