Analysis of Metabolites from Purple Cleome Extract (Cleome rutidosperma Linn.) as Potential Organic Fungicides
Analysis of Metabolites from Purple Cleome Extract (Cleome rutidosperma Linn.) as Potential Organic Fungicides
Ali Ikhwan1*, Dian Indratmi1, Faridlotul Hasanah1, Manar Fayiz Mousa Atoum2,3 and Irum Iqrar4,5
ABSTRACT
One disease that often attacks chili (Capsicum L.) is anthracnose caused by Colletotrichum capsici [(Syd.) E.J. Butler & Bisby]. In the field, C. capsici is controlled with a chemical fungicide that harms the environment. The purple Cleome plant (Spider plant – Cleome rutidosperma Linn.) can be extracted and function as an organic fungicide for environment-friendly control. This research aimed to examine the type and concentration of purple cleome extract metabolites and understand their effectiveness in inhibiting C. capsici. Purple cleome leaf is extracted with 1:1 w/v absolute methanol and concentrated with 1:1 v/v cold absolute methanol, and then the supernatant is purified using a centrifuge. The metabolic analysis was performed using Gas Chromatography-Mass Spectrometry (GC-MS) and comparing the data with the National Center for Biotechnology Information (NCBI) database to obtain specific metabolites. The inhibition test was done with one control (P0: chemical fungicide) and two-level of treatment, i.e., the concentration of purple Cleome extract (P1: 80 % and P2: 40 %), then ANOVA and HSD test were carried out to find out the best treatment. The purple Cleome leaf extract contained three metabolite compounds as organic fungicides, namely propanoic acid 4.12 %, phenol 8.38 %, and isopropyl myristate 15.86 %. The inhibition test confirmed that 80 % (P2) purple cleome extract could suppress C. capsici attack up to 16.67 %, significantly higher than chemical fungicide (24.4 %).
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August 2021
Agricultural Productivity and Sustainability Improvement in Tropical Region
