The Role of Aloe vera L. Extract in Modulating Oxidative Stress and Inflammation in Wistar Rats on a High-Fat Diet: Insights from In Vivo and In Silico Studies
The Role of Aloe vera L. Extract in Modulating Oxidative Stress and Inflammation in Wistar Rats on a High-Fat Diet: Insights from In Vivo and In Silico Studies
Roy Sukbir Singh1, Jekson Martiar Siahaan2,3*, Endy Juli Anto4, Syafruddin Ilyas5, Putri Eyanoer6, Hendrika Andriani7,8
ABSTRACT
Hyperlipidemia is a major health problem that is connected to cardiovascular events. It is characterized by disruptions in lipid profiles and is frequently accompanied by endothelial dysfunction, oxidative stress, and inflammation. Aloe vera, renowned for its secondary metabolites, is suggested as a potential treatment because of its hypolipidemic, antioxidant, and anti-inflammatory qualities. This study employed both in-silico and in-vivo approaches to explore these effects. The in-silico analysis involved molecular docking simulations to evaluate the interactions of Aloe vera compounds with inflammatory mediators such as TNF-α and COX-2. Furthermore, this study also employed a laboratory design with a post-test randomized controlled group. The study included 30 male white rats divided into six groups. Prior to the study, the rats were induced with a high-fat diet (HFD) for 14 days. The groups consisted of a normal group receiving distilled water, a negative control group following a high-fat diet, a positive control group treated with cholestyramine (200 mg/kgBW/day), and three experimental groups receiving Aloe vera at doses of 200, 300, and 400 mg/kgBW/day. At the end of the study, lipid profiles, MDA (malondialdehyde), and Hs-CRP (high-sensitivity C-reactive protein) levels were assessed. The in silico results indicated strong binding affinities for aloin in TNF-α, and Aloinoside in COX-2, this suggesting its potential inhibition of these targets. Moreover, at doses of 400 mg/KgBW Aloe vera extract had a significant effect improving their lipid profiles, and reducing MDA activity and inflammation (P < 0.05) with significance measured using ANOVA followed by Tukey’s Multiple Comparison Test. Hence, the ethanolic extract of Aloe vera has demonstrated efficacy as a hypolipidemic, and anti-inflammatory agent.
Keywords | Aloe vera, Anti-inflammatory, Lipid, In Vivo, In Silico
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