Recent Advances in Aptamer Based Biosensors for Detection of Antibiotic Residues
Recent Advances in Aptamer Based Biosensors for Detection of Antibiotic Residues
Atul Sharma, Aruna Chandra Singh, Gautam Bacher, Sunil Bhand
ABSTRACT
Antibiotics are widely used as bacteriostatic or bactericidal agents either to kill or inhibit the growth of microorganisms. Their abuse results in various side effects on the human health, environment and agriculture. The occurrence of bacterial “suprainfection” with tolerance to antibiotics has attracted the significant attention, mainly due to the consequences of multi drug resistance and potential threat to human health and the environment. With the increasing incidences of antimicrobial contamination, especially in food, dairy products, agriculture and environment, their regular monitoring is on prime interest. Aptamers are synthetic short sequences of single stranded (ss) oligonucleotides (ss-RNA or DNA), which are developed by an in-vitro selection process known as “Systematic Evolution of Ligands by Exponential Enrichment (SELEX)” technique. Among the receptors available for biosensing, aptamer exhibit the advantages of high specificity, selectivity, stability, facile labelling and modification, which makes them ideal candidates for development of new biosensing applications for detection of specific target molecules. In the present review, we concentrate on the recent advances in the development of aptasensors for antibiotics residue analysis based on electrochemical signal generation. Aptamers possesses the strong potential as receptors for the development of biosensors for antibiotics detection; therefore, a specially designed aptamer specific to an antibiotic may be suitable for this purpose. In this review, the importance of detection of antibiotic residue contamination, reported analytical methods, advancement in biosensing platform especially regarding electrochemical transduction are discussed in detail. Finally, future prospects toward the development of selective and sensitive aptasensors for antibiotic detection are presented.
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