Effects of Carbon Sources and Growth Regulators on the Tissue Culture of Sugarcane
Effects of Carbon Sources and Growth Regulators on the Tissue Culture of Sugarcane
Mazhar Ullah and Mohammad Sayyar Khan*
ABSTRACT
Tissue culture protocols with enhanced efficacy are prerequisite for the effective micro-propagation of sugarcane. Various factors affecting tissue culture must be evaluated and optimized to produce maximum callus and regenerated plantlets. In this research, different auxins [Dichlorophenoxy Acetic acid (2,4-D), Nephthalene Acetic Acid (NAA)], cytokinin [Benzyl Amino Purine (BAP)] either alone or in combination with each other and carbon sources [sucrose, glucose and fructose] with varying concentrations (2, 4 and 6%) were evaluated for their effect on the tissue culture of sugarcane variety-CP 77/400. For callus induction 2,4-D as an auxin was found to be most effective as compared to NAA. BAP and NAA in combination resulted in high regeneration capacities. Amongst the carbon sources, sucrose was most effective both for callus induction and regeneration. ANOVA revealed significant differences (P ≤ 0.05) amongst the callusing media (CM). Maximum callus induction (48.55%) was achieved on CM-2 augmented with 2.5 mg L-1 2,4-D and 4% sucrose. All the carbon sources at 4% concentration in CM-2 showed maximum callus induction. However, the best results were shown by sucrose with 50.33% callus induction. Significant differences (P ≤ 0.05) were observed amongst the different shooting media (SM). Maximum regeneration (72.06%) was observed on SM-2 supplemented with BAP (2 mg L-1), NAA (0.25 mg L-1) and 6% sucrose. Different carbon sources at 6% concentration in SM-2 showed high regeneration however sucrose resulted in maximum shoot regeneration (77.98%). Roots were established on ½ MS having 1.5 mg L-1 NAA and regenerated plantlets were transferred to silt and clay soil (1:1) and successfully acclimatized to greenhouse condition.
To share on other social networks, click on any share button. What are these?
