Purification and Partial Characterization of Superoxide Dismutase from Kidney of Hypophthalmichthys molitrix under Exposure of Metals Mixture
Purification and Partial Characterization of Superoxide Dismutase from Kidney of Hypophthalmichthys molitrix under Exposure of Metals Mixture
Anum Rafique, Sajid Abdullah, Khalid Abbas, Huma Naz* and Wardah Hassan
ABSTRACT
In this research work Superoxide dismutase (SOD) enzyme from the kidneyof silver carp(Hypophthalmichthys molitrix) was studied. Fish was divided into two groups. One group kept under chronic exposure of metals mixture (Pb+Cr) for 15 days and other group of fish was kept under controlled conditions. Partial purification of SOD was done by Ammonium sulphate precipitation. Ion-exchange column chromatography was used to purify enzyme for further partial characterization against different range of temperature and pH. As a result of ion exchange chromatography enzyme from kidney of control and Pb+Cr treated fish had highest activity as 508.33 and 427 UmL-1 while highest specific activity was 1105.06 and 1055.55 Umg-1 with 1.98 and 2.70 fold purification, respectively. Recovery was 70% and 68% in kidney of control and Pb+Cr treated fish, respectively. After characterization it was observed that SOD had wide range of pH i.e. 4.0-8.5 and maximum activity of 517 and 570 UmL-1 for control and treated fish kidney at pH 7.5 and 6.5, respectively. It was also observed that activity of enzyme increase with increase in temperature but upto a certain limit. At 40°C and 50°C activity of SOD was increased for unstressed and stressed kidney of H. molitrix i.e. 525 and 550 UmL-1, respectively. At p<0.01 all results were statistically significant.
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June 2018
Vol. 50, Iss. 3, Pages 799-1198
