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Quality Evaluation of Reference Gene Expression on Different Tissues in Adults of Tropical Gar Atractosteus tropicus

Quality Evaluation of Reference Gene Expression on Different Tissues in Adults of Tropical Gar Atractosteus tropicus

Luis Daniel Jiménez Martínez1, Vicente Morales Garcia2Carlos Alfonso Frias Quintana3, Alejandra del Carmen Castillo Collado1, Gloria Gertrudys Asencio Alcudia4, Carina Shianya Alvarez Villagomez4, Emyr Saul Peña Marín4,5, Bartolo Concha Frias1 and 
Carlos Alfonso Alvarez-Gonzalez4

1Laboratorio de Biología Molecular, DAMJ-UJAT, Dirección: Carretera Estatal Libre Villahermosa-Comalcalco Km. 27+000 s/n Ranchería Ribera Alta, C.P. 86205, Jalpa de Méndez, Tabasco, Mexico.
2Laboratorio de Docencia, DAMC-UJAT, Dirección: Rancheria Sur cuarta sección Comalcalco, Tabasco, Mexico.
3Laboratorio de Investigación en Biotecnologia Acuicola (LIBA), Tecnologico Nacional de Mexico Campus Boca del Rio (ITBoca). Boca del Rio, Veracruz, Mexico.
4Laboratorio de Acuicultura Tropical, DACBIOL-UJAT, Carretera Villahermosa-Cárdenas Km 0.5, C.P.86139 Villahermosa, Tabasco, Mexico.
5Cátedra-CONACyT, CDMX, Mexico.
 

*      Corresponding author: alvarez_alfonso@hotmail.com 

ABSTRACT

Tropical gar (Atractosteus tropicus) is an ancestral subtropical fish species in southeastern Mexico, which has great potential as a model species for physiological, biomedical and genomic studies. The quantification of gene expression through RT-qPCR is one of the most commonly used techniques, due to its precision, sensitivity and high performance, particularly in gene expression to compare between cells, tissues and organs; as well as different populations, stages of development, metabolism, among other conditions. This study analyzed the stability and normalization of six commonly used reference genes such as alpha elongation factor (ef1-α), beta-actin (actb), 18S ribosomal RNA (18s rrna), beta-2-microglobulin (b2m), tubulin alpha (α-tub) and glyceraldehyde 3-phosphate dehydrogenase (gapdh) in the different tissues of the intestine, muscle, gill, stomach, brain and liver in adult males of A. tropicus from reared in captivity through three BestKeeper, geNorm and NormFinder algorithms. Based on our results we can conclude that in the three BestKeeper, NormFinder and geNorm algorithms, the most stable genes are ef1, followed by 18s rrna and actb where the gene stability will depend on specific tissue to analyze in tropical gar A. tropicus adults.
 

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Pakistan Journal of Zoology

February

Vol. 54, Iss. 1, Pages 1-501

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