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The Influence of Differential Administration of Natural Vitamin E (Green Bean Sprouts) and Synthetic Vitamin E on the Macroscopic and Microscopic Quality of Semen in Sheep

The Influence of Differential Administration of Natural Vitamin E (Green Bean Sprouts) and Synthetic Vitamin E on the Macroscopic and Microscopic Quality of Semen in Sheep

Kusuma Adhianto*, Tina Rahmawati, Rio Ramanda, Muhtarudin Muhtarudin, Sri Suharyati 

Department of Animal Science, Faculty of Agriculture, University of Lampung, Jl. Prof.Dr. Soemantri Brojonegoro No.1 Gedong Meneng Bandar Lampung 35145, Lampung Province, Indonesia.

*Correspondence | Kusuma Adhianto, Department of Animal Science, Faculty of Agriculture, University of Lampung, Jl. Prof.Dr. Soemantri Brojonegoro No.1 Gedong Meneng Bandar Lampung 35145, Lampung Province, Indonesia; Email: kusuma.adhianto@fp.unila.ac.id 

ABSTRACT

The objective of this research was to investigate the impact of administering natural vitamin E (from green bean sprouts) and synthetic vitamin E on the macroscopic (volume, viscosity, color, odor, and pH) and microscopic (motility, live spermatozoa, abnormalities, and concentration) quality of sheep semen. The research involved an experimental study using a Randomized Complete Block Design (RCBD) consisting of 3 treatments, with each treatment being replicated 5 times. The treatments applied were as follows: P0; Complete Feed 60% + silage 40% (without the addition of natural and synthetic vitamin E), P1; Complete Feed 60% + Silage 40% + Natural Vitamin E 50 IU (equivalent to 223 grams of green bean sprouts), P2; Complete Feed 60% + silage 40% + Synthetic Vitamin E 50 IU. The acquired data was subsequently analyzed using descriptive methods for pH, consistency, color, and odor, while volume, motility, live spermatozoa, abnormality, and concentration were analyzed using ANOVA and followed by Bonferroni post hoc test at a significance level of 5%. The findings of this study indicate that there is no significant (P>0.05) difference in pH, consistency, color, and odor between natural vitamin E and synthetic vitamin E supplemented groups, and they do not significantly impact the motility, live spermatozoa, abnormalities, and semen concentration. However, there is a significant (P<0.05) difference in semen volume. The study concludes that inclusion of synthetic or natural vitamin E in sheep ration does not have an impact on the macroscopic and microscopic quality of the semen. 

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Pakistan Journal of Zoology

August

Pakistan J. Zool., Vol. 56, Iss. 4, pp. 1501-2000

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