Toxicity Analysis of Local Isolates of Bacillus thuringiensis and CRY1F Protein against Selected Lepidoptera Pests
Toxicity Analysis of Local Isolates of Bacillus thuringiensis and CRY1F Protein against Selected Lepidoptera Pests
Yusra Karim1, Munawar Saleem Ahmad1, Javed Khan2, Imtiaz Khan3*, Said Hussain Shah2, Syeda Anika Shamsher4, Imran Qazi5, Habib-Ur-Rehman Kakar6 and Wajih Ullah7
ABSTRACT
Bacillus thuringiensis has the potential to control a variety of insect pests, particularly the species of Lepidoptera. The goal of the current investigation was to evaluate the bio larvicidal effects of endotoxins produced by B. thuringiensis from the CRY1F gene against widely encountered lepidopteran pests (Spodoptera litura and Helicoverpa armigera). Insect-proof netting was used to contain the vegetable plants in a small field. B. thuringiensis with active CRY1F was cultivated in Luria-Bertani (LB) media under controlled laboratory conditions. The goal was to maximize colony growth for the preparation of spore/crystal mixtures at various concentrations. The larvae of S. litura and H. armigera were reared in a laboratory under control. Following tests, the LC50 (96-hours) of the CRY1F protein-crystal mixture against lepidoptera pests was determined to be 158.37 µg/ml for S. litura and 170.73 µg/ml for H. armigera. The endotoxin mixes of CRY1F exhibit considerable potency, causing 100% mortality in S. litura with 500 µg/ml and H. armigera with 600 µg/ml.. The overall findings showed that the Bt local isolates and CRY1F protein endotoxins were effective against both larvae. The bio larvicidal action of Bt local isolates and CRY1F endotoxins against various lepidopteron pests indicate promising results for pest control.
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