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Tellurite reduction potential of bacteria isolated from industrial wastewater

Tellurite reduction potential of bacteria isolated from industrial wastewater

 Asma Akhtar, Abdul Rehman

Department of Microbiology and Molecular Genetics, University of the Punjab, New Campus, Lahore-54590, Pakistan 

ABSTRACT

The aim of the present study was to isolate tellurite resistant acteria from industrial wastewater. Two bacterial isolates were identified as Staphylococcus epidermidis and Staphylococcus lactis on the basis of biochemical tests and 16S rRNA sequence. The minimum inhibitory concentration of both isolates showed fair growth up to 1 mM of K2Te2O3. Both strains showed maximum growth at 37ºC and pH of 7. The bacterial growth in the presence of metal ions was delayed as compared to the control. High ellurite reduction potential was shown 98% (1f) and 97% (2b) after 24 h of incubation. Glucose 6 phosphate dehydrogenase (G6PDH) activity was increased to 279% (1f) and 150% (2b) when ompared to the respective control indicating the ability to tolerate tellurite tress. Differential pattern of bacterial proteins in tellurite stress was obtained by SDS-PAGE. Both bacterial strains can be utilized to convert toxic tellurite into its elemental form from the contaminated sites.

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Punjab University Journal of Zoology

December

Vol.38, Iss. 2, Pages 137-236

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