Amna Qazi1, Ghulam Shah Nizamani2*, Muhammad Tahir Khan2, Shafquat Yasmeen2, Shahla Karim Baloch1, Muharram Ali1, Imtiaz Ahmed Khan2, Sagheer Ahmad3, Muhammad Rashid Nizamani4 and Mohammad Aquil Siddiqui2
Micropropagation is a phenomenal technique to serve rapid multiplication of vegetatively propagated plants through in vitro culture. This study was conducted to establish the optimal concentrations of plant growth regulators and sucrose for micropropagation of sugarcane. Sugarcane is cultivated through cane sets and therefore, multiplication of any new genotype through traditional practices requires interminable period of time. Eight sugarcane genotypes viz. NIA-2004, SPF-234, NIA-2012, BL4, AEC92-1208, Thatta-10, Gulabi-95 and NIA-1026-P7 were subjected to tissue culture using four different combinations of shoot induction media and three disparate recipes of rooting media. It was seen that both of the factors i.e. genotype as well as growth medium played significant role in inducing propagation of the sugarcane. Shoots were observed to initiate earliest in NIA-2004, NIA-2012 and NIA-1026-P7 in MS media supplemented with 1.00 mg l-1 IAA + 1.00 mg l-1 Kin + 1.00 mg l-1 BAP and 20 g l-1 sucrose. Regarding number of shoots, shoot length and number leaves of the plantlets, BL4 was seen to produce excellent results in MS media containing 1.00 mg l-1 IAA + 1.00 mg l-1 Kin + 1.00 mg l-1 BAP and 20 g l-1 sucrose. Mean number of shoots, leaves and shoot length, were all recorded to be highest for this composition of media (1.42, 1.54 and 3.90, respectively). The results indicated critical role of BAP and Kin in shoot development and elongation. On rooting, profuse root formation was observed in MS media supplemented with 3.00 mg l-1 IBA + 4.00 mg l-1 NAA and 30g l-1 sucrose. All of the varieties showed root development under this composition except NIA-1026-P7. The results of this experiment also showed that IBA and NAA are vital towards root formation. Moreover, it was seen to be evident that optimal media concentrations for micropropagation varied from genotype to genotype. Establishment of mature micropropagation protocols for new elite sugarcane cultivars can help in rapid multiplication of disease-free potential material to occupy large area in short time span, giving the phenomenon paramount economic importance.
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