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Expression profile of Toll-like Receptors Pathway Genes in Chicken Erythrocytes Infected with Mycoplasma synoviae

Expression profile of Toll-like Receptors Pathway Genes in Chicken Erythrocytes Infected with Mycoplasma synoviae

Xin-yu Han1,2, Afrasyab Khan1,2, Ali Raza Jahejo1,2, Qian-qing Cheng1,2, Meng-li Qiao1,2, Raza Ali Mangi1,2, Muhammad Farhan Qadir1,2, Ding Zhang1,2, Ying Wang1,2, Yu-hai Bi1,3, Rui-wen Fan1,2 and Wen-xia Tian1,2

1College of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030801, China
2Shanxi Key Laboratory of protein structure determination, Shanxi Academy of Advanced Research and Innovation, Taiyuan 030032, China
3CAS Key Laboratory of Pathogenic Microbiology and immunology, Collaborative Innovation    Center of Infectious Diseases, Institute of Microbiology, Center for Influenza Research and Early-Warning (CASCIRE), Chinese Academy of Science, Beijing 100101, China 
Xin-Yu Han and Afrasyab Khan have contributed equally to this study.
* Corresponding author:


Toll-like receptors (TLRs) are one of the important immunes signaling pathways that participate in the activation of host immune response after detecting microbial pattern molecules. Despite important expression profile TLRs genes in chicken erythrocytes, studies have been lacking. This study investigated the expression profile of TLRs pathway immune gene in chicken erythrocytes in response to Mycoplasma synoviae. The purpose of the current in-vitro study was to determine the chicken erythrocytes interaction with M. synoviae using a transmission electron microscope (TEM). The mRNA gene expression of TLR1, 2, 3, 4, 5, 7, 15, MHC I, II, and MyD88 in M. synoviae infected chicken erythrocytes was determined using quantitative real-time PCR (qRT-PCR) at four different time intervals (0, 2, 6 and 10 h) post-infection and compared to uninfected controls. The mRNA expression of TLRs such as TLR1, 2, 3, 15, and MHC II were significantly upregulated at 6 and 10 h post-infection in infected chicken erythrocytes. However, significantly upregulated expression of TLR5 and MHC I were noted at 2, 6, and 10 h while TLR4 and MyD88 mRNA expression was also significantly upregulated but at different time intervals. This study provides the first evidence of upregulated expression of TLR signaling pathway genes in M. synoviae infected chicken erythrocytes. These results provide new insights on M. synoviae infection resistance mechanisms and the role of TLR signaling immune genes in the control of the host immune response.

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Pakistan Journal of Zoology


Pakistan J. Zool., Vol. 56, Iss. 1, pp. 01-501


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