Anti-Stress Activity of Oviductus Ranae in Mice with Acute Stress Based on Network Pharmacology
Anti-Stress Activity of Oviductus Ranae in Mice with Acute Stress Based on Network Pharmacology
Zhe Lin1, Yumo Li1, Yuchen Wang1, Yuechen Li1, Ke Pei1, Guangfu Lv2, He Lin1* and Zhun Yu3*
ABSTRACT
Stress is a state of threatened homeostasis that causes the body to release the hormone cortisol produced by the adrenal glands. Oviductus ranae (OR) is an animal based raw material of folk medicine which plays a variety of activities. However, its anti-stress effects mechanism has not been fully revealed. In this work, based on network pharmacology, the potential targets of OR were screened, and an protein-protein interaction (PPI) network between the target of OR and anti-stress target was constructed using STRING database. Kyoto Encyclopedia of Genes and Genomes (KEGG) was used for analyzing the pathways of target gene. To further verify this, total 96 ICR mice were used, forced swim test and anoxic tolerance test were performed. The effect of OR on levels of monoamine neurotransmitters and phosphorylation of p38 which closely related to anti-stress were examined. The results showed that, 203 potential OR targets and 126 stress-related gene targets were obtained, in which there were 15 common targets. Pathway enrichment analysis showed that there were 20 critical pathways. The results revealed that OR could increase the total swimming time, increase the survival time of enduring anoxia, and regulate monoamine neurotransmitters such as 5-hydroxytryptamine (5-HT), 5-hydroxyindoleacetic acid (5-HIAA), norepinephrine (NE) and dopamine (DA). Western blot analyses indicated that OR may decrease the phosphorylation of p38. In conclusion, the results revealed that OR may play the anti-stress effects by inhibiting mitogen-activated protein kinase (MAPK) pathway, thus promote the normalization of acute stress. This study revealed the possible mechanism of OR as a potential material for the treatment of acute stress-related problems, and laid a foundation for the further development and utilization of OR.
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