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In-vitro Susceptibility of Mycoplasma capricolum Subsp. capripneumoniae Pakistan Strain to Commercially Available Quinolones

In-vitro Susceptibility of Mycoplasma capricolum Subsp. capripneumoniae Pakistan Strain to Commercially Available Quinolones

Mumtazur Rahman1, Farhan Anwar Khan1,*,Umar Sadique1, Ijaz Ahmad1, Shakoor Ahmad1, Faisal Ahmad1,2, Hayatullah Khan1,3, Muhammad Saeed1, Faiz Ur Rehman1, Ibrar Hussain1, M. Faraz Khan1, M. Izhar ul Haque1 and Hanif-ur-Rehman1,3

1College of Veterinary Sciences, Department of Animal Health, Faculty of Animal Husbandry and Veterinary Sciences, 25120, The University of Agriculture, Peshawar, Khyber Pakhtunkhwa,
2Directorate of Livestock and Dairy Development (Extension), Peshawar, 25000, Khyber Pakhtunkhwa
3Directorate of Livestock and Dairy Development (Research), Peshawar, 25000, Khyber Pakhtunkhwa

*      Corresponding author: farhan82@aup.edu.pk

ABSTRACT

Contagious caprine pleuropneumonia (CCPP) caused by Mycoplasma capricolum subsp. capripneumoniae (Mccp) is a highly contagious and deadly disease of small ruminants, especially goats. It is an economically important disease, causing high morbidity and mortality in goats. More recently Mccp Pakistan strain has been identified and isolated by our laboratory. Alarmingly, antimicrobials in animals are inappropriately using and resultantly leading to antimicrobial resistance (AMR) including mycoplasmas, which are causing serious infections in ruminants. Since the growth curve of our local strain and its susceptibility to commonly using antimicrobials is unknown. Therefore, this study was aimed to evaluate the growth curve and susceptibility of Mccp against commercially available quinolones in Pakistan by broth microdilution method. Quinolones including moxifloxacin, levofloxacin, ciprofloxacin, and enrofloxacin were selected for in vitro susceptibility of Mccp Pakistan strain. The concentration for each drug was measured distantly depending on high concentration available in the market and was then used in various concentrations ranges from higher to lower i.e. moxifloxacin 80-0.156 µg/ml, levofloxacin 250-0.488 µg/ml, ciprofloxacin and enrofloxacin 50-0.097 µg/ml. The resultant effective antimicrobial by minimum inhibitory concentration (MIC) and minimum mycoplasmacidal concentration (MMC) value was ciprofloxacin having lowest MIC and MMC value (0.390 µg/ml and 0.781 µg/ml), respectively. It was concluded that ciprofloxacin had best mycoplasmacidal and mycoplasmastatic activities among tested chemotherapeutics agents against Mccp. Ciprofloxacin could be more effective antimicrobial in field against Mccp infection in goat.
 

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Pakistan Journal of Zoology

December

Vol. 53, Iss. 6, Pages 2001-2521

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