Effect of Caffeine Citrate with Aminophyline on Apoptosis and Respiratory Endothelial Cell Dysfunction in Premature Infants
Effect of Caffeine Citrate with Aminophyline on Apoptosis and Respiratory Endothelial Cell Dysfunction in Premature Infants
Hongfeng Guo
ABSTRACT
This study was aimed to investigate the mechanism of continuous positive airway pressure (CPAP) combined with caffeine citrate and aminophylline in the treatment of premature infants with apnea. in present study, sixty premature SD mice were randomly divided into blank control group (n = 20), intermittent hypoxia group (n = 20) and drug administration group (n = 20). Establishment of intermittent hypoxia model: the oxygen concentration in the cabin of mice in intermittent hypoxia group was maintained at 40%, then decreased to 10% and maintained for 3 min. After that, it was continuously increased to 40%, and was decreased to hypoxia after 8 min. The operation was repeated 3 times every 3h. The drug administration group was treated with continuous CPAP during modeling. The expressions of malondialdehyde (MDA), superoxide dismutase (SOD) and prostaglandin (PGE) in lower respiratory tract were detected by the kit; the expressions ofprotein kinase B (Akt), Bax and Bcl-2 were detected by Western blot. According to our findings after drug treatment, the body weight and respiratory ventilation of mice returned to normal level compared with the intermittent hypoxia group. Compared with the blank control group, after intermittent hypoxia, the contents of MDA, SOD and PGE in the lower respiratory tract of SD mice were significantly lower than those in the normal air group (P<0.05); after the premature mice were treated with drugs, the contents of MDA, SOD and PGE in the lower respiratory tract of SD mice were almost normal compared with those in the intermittent hypoxia group. RT-PCR results showed that compared with the blank control group, the expression of VEGF gene in the lower respiratory tract of SD mice increased after intermittent hypoxia (P<0.05). WB results showed that compared with the blank control group, the expressions of Akt and Bax protein in the lower respiratory tract of SD mice in intermittent hypoxia group were increased. The expression of Bcl-2 was decreased, and the difference was statistically significant (P<0.05). for conclusion, CPAP combined with caffeine citrate and aminophylline could improve the expressions of Akt, Bax and bcl-2 protein to treat premature infants with apnea.
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