Chemoprotective Effect of Daphnetin against Benzene Induced Leukemia Cancer via Alteration of CYP2E1
Chemoprotective Effect of Daphnetin against Benzene Induced Leukemia Cancer via Alteration of CYP2E1
Junling Liu, Chen Sun, Qiao Yu, Yanzhi Liang, Shanshan Lin and Meng Tian*
ABSTRACT
Leukemia is a malignant disease of blood forming tissue inducing the over-production of large number of immature blood cells that enter the peripheral blood. Leukemia considered as the 9th most common cancer in men and 12th in women. Available treatment for leukemia are chemotherapy, allogeneic cell transplantation and radiation therapy with side effects. Due to side effect linked with the treatment, medicinal herbs treatment having the more attraction to treat the leukemia. The current study was to scrutinize the anti-leukemic effect of daphnetin against benzene induced leukemia in rats and explores the underlying mechanism. Benzene was used for the induction of leukemia in experimental rats. The rats were divided into different groups and received the daphnetin (12.5, 25 and 50 mg/kg). The body weight, haematological parameters, deoxyribonucleic acid (DNA) fragmentation and cell cycle regulatory parameter were also estimated. Reverse transcription polymerase chain reaction (RT-PCR) was used for the estimation of messenger RNA (mRNA) expression of cytochrome P450 2E1 (CYP2E1). Daphnetin treated rats showed the up-regulation of body weight as compared to other groups. Moreover, daphnetin reduced the blasts in leukemic rats. It also altered the hemotological parameters such as red blood cell (RBC), white blood cell (WBC), lymphocytes, neutrophils, monocytes, eosinophils, monocytes and basophils, respectively. Daphnetin treated rats showed the increased level of p21 and p53 and reduced level of cyclins D1 and E. RT-PCR showed the up-regulated of mRNA expression of CYP2E1 of daphnetin treated group rats as compared to other groups. The current study showed the anti-leukemic effect of daphnetin and highlights the possibility of its use in leukemia to minimize the side effect of the usual therapy.
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