Sehrish Kanwal1, Ali Saeed1*, Muhammad Munir2, Memoona Arshad1
Life Sciences International Journal Issue 7 Volume 1
...bstract | Foot and mouth disease (FMD) remained an endemic disease in Pakistani livestock population during 2011 and 2012. To ascertain the genetic nature of circulating FMD virus, samples were collected from suspected outbreaks in Southern Punjab. The virus was primarily detected with RT-PCR with P1/P2 and 1F/1R universal primer pairs directly from field samples including tissues, vesicle and secretion. The direct sequencin...
Sehrish Kanwal, Ali Saeed, Muhammad Munir, Memoona Arshad
British Journal of Virology
...justify;">Foot and mouth disease (FMD) remained an endemic disease in Pakistani livestock population during 2011 and 2012. To ascertain the genetic nature of circulating FMD virus, samples were collected from suspected outbreaks in Southern Punjab. The virus was primarily detected with RT-PCR with P1/P2 and 1F/1R universal primer pairs directly from field samples including tissues, vesicle and secretion. The direct sequenci...
Johanna Lindahl 1, 2
British Journal of Virology
... quoted as important for disease emergence. Several of the mechanism by which urban disease emergence is promoted also increase the risks of vector-borne diseases, and the mosquito-borne Dengue virus is one of the pathogens benefitting from this. The related virus Japanese encephalitis virus is the most important cause of mosquito-borne encephalitis in the world, and is maintained in avian...
Sehrish Kanwal1, Ali Saeed1*, Muhammad Munir2, Memoona Arshad1
British Journal of Virology
...on....
El-Sayed M. Abdelwhab, Jutta Veits and Thomas C. Mettenleiter
Avian Influenza H5N1 in Egypt: What we Know and What we have to Know?
...ationwide to control the disease in poultry. Meanwhile, the viruses accumulated several point mutations in the HA immunogenic epitopes resulting in antigenic drift and the establishment of infections in vaccinated poultry. The Egyptian H5N1 viruses remain susceptible to oseltamivir, but genetic markers indicating resistance to amantadine have increased dramatically. In this mini-review, we discuss recent findings on the H5N1 viruses endemic in poultry and huma...
Muhangi Denis
...highly infectious, viral disease affecting domestic pigs with mortalities approaching 100%. The disease severely impacts on the socio-economic status of farmers. The disease is endemic in a number of countries in sub-Saharan Africa, in Sardinia (Italy), the Russian Federations, the Caucasus and it has shown possibilities of spread within Eastern Europe. Involvement of all stakeholders in t...
Yanina Panzera1*, Nicolas Sarute1, Lucia Carrau1, Jaime Aldaz2, Ruben Perez1
...t significant infectious disease among carnivores worldwide. Herein, we present the state of the art of CDV dynamics in South America, by means of a detailed analysis of the genetic diversity, evolution, and epidemiology of the virus in several countries. Our study allowed us to establish the presence of four genetic lineages in South America, being the continent with more lineages described to date, underscoring the high genetic diversity of the circulating s...
Nicola J. Stonehouse
...merase of foot-and-mouth disease virus. The aptamers are able to inhibit the function of the enzyme both in vitro and in the context of a sub-genomic replicon and highlight the future potential of this technology in many aspects of virus research as well as in diagnostics and therapeutics....
Xingdong Yang and Lijuan Yuan
...tudies of the infection, disease and immune responses caused by a specific pathogen in the absence of interfering maternal antibodies, other maternal immune regulators, and intestinal and environmental microbes. Due to the similarities between pigs and humans in terms of genetics, physiology, intestinal anatomy, and immune system, pig is particularly a suitable animal species for the modeling of human enteric pathogen infections, immune responses and for vacci...
Matheus Nunes Weber1, Simone Silveira1, André Felipe Streck1, Luís Gustavo Corbellini2, Cláudio Wageck Canal1*
...vine viral diarrhea is a disease caused by viruses of the genus Pestivirus in cattle worldwide. Herein, we present the current status of the disease in Brazil showing its nation-wide distribution. In Brazil, BVDV-1, BVDV-2 and ‘HoBi’-like viruses are frequently reported whereas BVDV-2 has a higher frequency when compared with other continents. It is important to reinforce that currently Brazil has no official con...
Simon Mwangi Kihu1*, George Chege Gitao1, Lily Caroline Bebora1, Njenga Munene John1, Gidraph Gachunga Wairire2, Ndichu Maingi1, Raphael Githaiga Wahome1, Davis Njuguna Karanja1, Julius Otieno Oyugi3, Ernest Lutomia3
...ly as 1995, the clinical disease was observed later in 2006 following dramatic and devastating PPR outbreaks in Turkana County. In this context, an outbreak of PPRV was observed in Tukana County in 2011 and the current study detail the clinical, pathological and laboratory findings of this outbreak. The disease was clinically manifested by the depression, diarrhea, difficult breathing, muco-purulent ocular-nasal discharges w...
James Ayukepi Ayukekbong1,2
E-mail | [email protected]
...ant mosquito-borne viral disease but there are currently no licensed vaccines or specific therapeutics. The virus and its vector are widely distributed throughout the tropics and subtropics regions of the world. Dengue and malaria are endemic in Nigeria. Two independent studies among febrile patients revealed dengue IgG seroprevalence of 73% and malaria prevalence of 80% in some areas of the country. The diagnosis of the disease...
Gerald Misinzo1,*, Tebogo Kgotlele1, Epaphras A. Muse1, Jan Van Doorsselaere2, Mikael Berg3, and Muhammad Munir4
...uses a highly contagious disease in both domestic and wild small ruminants, and in camels. Based on sequence analysis of the nucleoprotein (N) gene, the PPRV has been classified into four lineages. Serological investigations in Tanzania indicate that peste des petits ruminants (PPR) was introduced in 2004 in Ngorongoro district bordering Ken- ya before official confirmation of the disease in most districts of Northern Tanzan...
Paulo Eduardo Brandao
...obtained for other viral diseases. This review is intended to present the current situation of RNAi against rabies, as well as insights into the future on the field....
Nayle Maria Oliveira da Silva1*, Ricardo do Carmo Zanella2
...CV induces chronic liver disease, which is characterized by a persistent hepatic parenchyma inflammatory process that may progress to cirrhosis and hepatocarcinoma. Viral clearance occurs in a minority of patients with viral hepatitis C, whereas chronic infection is established in 60 to 80% of all cases, 20 to 40% of which may evolve to cirrhosis and hepatocarcinoma. Genetic differences among infected hosts can determine the progression and outcome of the viru...
Qingzhong Yu
...ication of the Newcastle disease virus. Recently, inserting a green fluorescent protein (GFP) gene upstream from the GE sequences of the viral genes and subsequent quantitative measurements of the GFP fluorescence intensity, we have concluded that the P and M junction region is the optimal insertion site for a high level of foreign gene expression by a NDV vector....
Suresh V Kuchipudi* and Kin-Chow Chang
...hysical demands, age and disease. The contributions of skeletal muscle to immune response and virus pathogenesis are increasingly recognized. Recent evidence strongly indicated that skeletal muscle cells fully support productive replication of influenza A virus (IAV) and could play an important role in disease outcome. We are of the opinion that skeletal muscle could be a key innate immune tissue/organ in the pathogenesis o...
Hu Zenglei1 and Xiufan Liu1, 2*
...thogenicity of Newcastle disease virus (NDV) is one of the major determinants for the clinicopathological manifestations in infected hosts. Recent studies showed that the host innate immune responses to infection play important role in the pathologenesis of NDV, and correlate with the severity of the clinical disease. Virulent strains of different genotypes induce distinct pathological manifestations in chicken organs, espec...
Leonard Izzard1, 2, John Stambas1, 2*
...ty and as biomarkers for disease severity....
Josef D. Jarhult1, 2
...ors for severe influenza disease as well as an important part of pandemic preparedness plans. Resistance development is a potential problem both in treated humans and in the environment. The use of oseltamivir for treatment of uncomplicated influenza in otherwise healthy patients should be questioned....
Guoyi Li1, Haiyan Tong1, Jiansheng Chen2, Jinliang Wei2, Xiaoming Zhao2, Guoping Zhang2, Zixue Shi2*
E-mail | [email protected]
...E) is an arthropod-borne disease causing dreadful central nervous system signs and high mortality in adults and children. It is caused by the JE virus (JEV), which belongs to the family Flaviviridae. JEV is endemic to many parts of East Asia, Southeast Asia and Australia, where periodic outbreaks take thousands of lives. With rapid globalisation and climatic shift, JEV has started to emerge in previously unaffected areas. Scientific evidences show tha...
Rodolfo Villagra-Blanco1*, Gaby Dolz1, Danilo Montero-Caballero2, Juan Jose Romero-Zuniga2
...e with this insect borne disease. The results of this study indicate that BTV is endemic in sheep herds from Costa Rica, and animals seem not show clinical signs. We recommend carrying out further studies, to determine the presence of BTV in goats and wild ruminants, and to identify serotypes present in the country....
Mohammed A. Rohaim1*, Rania F. El-Naggar2, Abdulrahman M. Gamal3, Elshaimaa Ismael3, Mohamed M. Hamoud4, Sherif T. Moubarak3, Ashraf M. Metwally1, Manal M. Zaki3, Shimaa A.E. Nasr3, Samah Elsaid3, Mohamed M. Ali3, Hussein A. Hussein1 and Osama K. Zahran3
Email: [email protected]; [email protected]
...ms along with infectious diseases including avian influenza (AI). Biosecurity measures, controlling poultry movements and inactivated vaccines were devised to combat the spread of highly pathogenic avian influenza (HPAIV) H5N1. HPAIV are highly susceptible to all disinfectants because they are enveloped viruses. Disinfection against avian influenza viruses at the poultry farms would significantly reduce and/or limit the chance for its trans...
Shumaila Manzoor1, Afshan Ahmed1 and Muhammad Abubakar2*
...SPCE) for foot and mouth disease virus (FMDV) structural antibodies detection in terms of their sensitivity and specificity. These methods were performed by using set of sera collected from cattle with foot-and-mouth disease vaccination. The pattern of antibody titers in animals of different age groups was observed as less than 1 year, 1-2 years and more than 2 years for both ELISA and SNT. The peak immun...
Youssef Lhor1*, Mounir Khayli1, Mohamed Bouslikhane2, Mehdi El Harrak3, Ouafaa Fassi Fihri2
Email: [email protected]
...ted control measures and disease risk analysis, this entomological survey was performed on the risk of transmission of bluetongue virus (BTV) and identification of the main breeding sites of Culicoides. From April 2009 to March 2010, the study was carried out at 14 sites distributed across the country. Results indicated that the two most abundant Culicoides species were Culicoides imicola (94.2-95.85%) and Culicoides newsteidi (2.21-2.72%)....
Tomas Jinnerot1*, Karin Malm1, Erik Eriksson1 and Jonas Johansson Wensman2
E-mail | [email protected]
...e infectious respiratory disease (CIRD) in dogs. Other disease agents include various respiratory viruses and co-infection can exacerbate the clinical signs. CIRD is highly contagious and can spread rapidly, especially among dogs in close quarters. Fast and accurate diagnostics enables the selection of proper management. Our objective was to develop a sensitive and specific real-time PCR for detection of ...
Claudia Kohl*, Andreas Nitsche, Andreas Kurth
Email: [email protected]
... yet unknown, infectious diseases and etiologies....
Shubhada K Chothe, Bhushan M Jayarao and Suresh V Kuchipudi
... (ncRNAs) to a number of disease processes including viral infections. Cellular and virus encoded lncRNAs are emerging as novel regulatory factors which can escape the anti-protein environment in the virus infected cells and are likely to be conserved across the species. Here, we summarize our current knowledge about the role of lncRNAs in influenza virus infection and the exciting prospect of exploiting lncRNAs as targets to develop novel anti-viral therapies...
Kang-Seuk Choi
...tify;">Infectious bursal disease (IBD) virus-like particles (VLPs) comprising only viral capsid proteins mimic the naive configuration of authentic IBD virus particles. The VLPs show intrinsic immunogenicity and a high safety profile. Thus, VLPs are considered one of the most promising approaches to vaccine development and are an alternative to inactivated IBD vaccines. In addition, VLP technology has many applications. This paper reviews the potential o...
Seyed Mohsen Mirbod
...osteoarthritis (OA) is a disease which influences the performance of the joint during walking. Various types of knee orthosis have been designed to improve the performance of the subjects, however, such cases are often associate with post-operational complications. The aim of this study is to design an orthosis which is easy to use, economical and effective. A new knee orthosis was designed to improve the performance of OA patients during standing and walking....
Rongfei Lu1, 2, Zhiyang Liu1, Peng Fang1, Guangyi Chen1, Feng Sun1, Yongjian Fan1, Yijun Zhou1, Tong Zhou1*
...d of virus detection and disease dynamics. The validity and the reliability of generated data can be enhanced significantly appropriate measures. As a standard for the relative expression of target genes, the selection of reference genes is crucial. This review describes the history of the RT-qPCR technology, emphasizes the importance of reference genes and enumerates several algorithms to screen reference genes to normalize the RT-qPCR data. Additionall...
Jonas Johansson Wensman1*a, Karl-Johan Leuchowius2,3 a, Jiting Yan1, Anna-Lena Berg4, Liv Bode5, Hanns Ludwig5, Sandor Belak6, Ulf Landegren2, Ola Soderberg2, Mikael Berg6
...t-align: justify;">Borna disease virus type 1 (BDV) comprises highly conserved neurotropic non-segmented negative strand RNA-virus variants causing neurological and behavioral disorders in a wide range of mammalian animals, possibly including humans. Viral persistence in the brain has been frequently observed, however, the exact mechanisms behind BDV’s ability to establish persistence despite a prominent immune response are not known. Here we have used i...
Behzad Hussain1, 2, Abdullah Iqbal2, Muhammad Abubakar2*
...an emerging deadly viral disease in Pakistan and is becoming endemic in many regions. The first case of CCHF was reported in 1976 in Rawalpindi and after that, multiple sporadic cases and outbreaks have occurred in subsequent years and till today. CCHF outbreaks have a case fatality rate of up to 40%, which is alarming. It is a zoonotic disease and the virus is transmitted mainly to the humans through hard tick bite and dire...
Anna-Maria Andersson*, Ann-Kristin Nyman, Per Wallgren
...span class="s1">Aleutian disease virus (ADV) infection in mink typically cause hypergammaglobulinemia, plasmacytosis and immune complex disease. Due to lack of success in eradicating Aleutian disease (AD) by stamping out, a new strategy for disease control with focus on evaluation of disease progression has been propos...
Moustafa Kardjadj
...lass="s1">Foot-and-mouth disease (FMD) is a highly infectious and contagious transboundary animal disease and one of the most economically devastating diseases that affect cloven-hoofed livestock including cattle, goats, sheep, pigs and wild ungulates. Wide prevalence of the disease in Asia and Africa associated with huge economic losses to livest...
Nadeem Khan1*, Arshad Ali Khan1, Mukhtar Ahmad2, Muhammad Nouman2 and Badshah Islam1
...ar (2.66 cm). In case of disease incidence maximum lesion on leaves were found in Kinnow (14.2) while in Cara Cara, Salustiana and Arnold Blood, lesser lesions were found. On the basis of results and overall performance Tarocco and Hamlin performed best in all aspects while Arnold Blood and Cara Cara revealed poor growth. ...
Ashley C. Banyard1* and Satya Parida2
...n economically important disease of small ruminants that causes extensive livestock losses across areas where it is endemic. Further the recent description of the disease in naïve populations of animals have demonstrated an expansion of the distribution of the disease. Having been in the shadow of rinderpest virus for decades little is understood regarding factors that influence the o...
Vinayagamurthy Balamurugan*, Gurrappa Naidu Govindaraj and Habibur Rahman
...tant transboundary viral disease of sheep and goats. PPR is enzootic in India as more number of outbreaks have occurred in the past and now occurring regularly, round the year and most frequently during the lean period throughout the country. In some Indian states viz. Andhra Pradesh, Karnataka, and Chhattisgar h the PPR outbreaks in sheep and goats have declined after implementing the strategic mass vaccination programme. The decreased number of outbreaks in ...
Moustafa Kardjadj1, Pam Dachung Luka2
...ed to be a transboundary disease of great significance through its effect on the development and maintenance of sustainable agriculture in developing countries, most notably in Western Africa and South Asia. Here, we review, using available data in research papers and reports of international organizations and databases, the factors that enables the spread of PPR in Africa and the required countermeasures that are required to control and eradicate it. However,...
Gurrappa Naidu Govindaraj, Vinayagamurthy Balamurugan*, Habibur Rahman
... highly contagious viral disease in small ruminants causing huge loss to farmers. In this study, an attempt has been made to estimate the economic losses based on the annual incidence, morbidity, mortality levels etc. derived from literature, discussion with experts, and based upon scientific facts. Different mathematical models were used to assess the losses due to mortality in young and adult sheep/goats, body weight loss in young and adult sheep/goats, milk...
Ahmed Zein Mahmoud1, Muaz Abdellatif 2, 3*, Luai Shazali1
...ess the situation of the disease in the region....
Amitha Reena Gomes1, Belamaranahally Muniveerappa Veeregowda2, Sonnahallipura Munivenkatappa Byregowda1, Vinayagamurthy Balamurugan3*
...nants (PPR), a viral disease of goats and sheep caused by a morbillivirus of the family paramyxoviridae is a major threat to small ruminant farming. Global announcement of PPR eradication by 2030 has opened lot of research gaps for the development of vaccines and diagnostics for differentiating infected and vaccinated animals. With the advent of recombinant DNA technology, recombinant protein based vaccines and/or diagnostics are being tested...
Klara Fischer1*, Erika Chenais1,2, Emeli Torsson1, Jonas Johansson Wensman1
... highly contagious viral disease of sheep and goats. Domestic sheep and goats are important species for the livelihoods of poor people in many developing countries. Within societies where PPR is now spreading, poverty is widespread and the disease is expected to have significant negative impacts on livelihoods. In resource-constrained marginalised societies, it is often difficult to collect disease
Keyvan Sharifmoradi1*, Nader Farahpour2
...s with Parkinson’s disease (PD) and to compare with healthy age group. A total of 14 patients with PD and 16 normal elderly subjects with matched age and gender were included in the study. A motion analysis system (Vicon Motion Analysis System) and two Kistler force plates were used to measure forces applied on the legs. Data were evaluated using independent t-test with significant point set at p< 0.05. There was no difference in cadence...
Mohammad Mushfiqur Rahman1, Rokshana Parvin1, Ataur Rahman Bhuiyan1, Mohammad Giasuddin2, Shah Md. Ziqrul Haq Chowdhury3, Mohammad Rafiqul Islam1, Emdadul Haque Chowdhury1*
... highly contagious viral disease of small ruminants. The disease is endemic in Bangladesh and causes considerable economic losses due to its high morbidity and mortality. The study was undertaken to detect and characterize the PPR virus (PPRV) circulating in Bangladesh. Twelve local isolates of Bangladeshi PPRV were successfully detected from post mortem samples (lymph node, spleen and trachea) of goats and a sheep during th...
Muhammad Abubakar1*, Shumaila Manzoor2, Jonas Johansson Wensman3, Emeli Torsson3, Qurban Ali1 and Muhammad Munir4
...eeks. Interestingly, the disease was exclusively observed in goats and all sheep kept in the same herd were serologically positive but did not show any clinical signs of PPR. The active PPR virus (PPRV) infection was confirmed by antigen capture ELISA and RT-PCR in both swab and body tissue samples. The molecular characterization revealed clustering of the PPRV within lineage IV with significant substitutions in the nucleoprotein (NP) gene. Genetic variations ...
Neha Singh and Anjana Pandey
...s, and to discover novel disease biomarkers to facilitate early detection of the disease and for therapeutic purposes....
Hassan Tarik Atmaca
...ically devastating viral disease of small ruminants and camels, and thus is negatively affecting the livelihoods of poor people in many developing countries. Efforts are being made to advance our understandings on different aspects of molecular biology of the virus and to consolidate national and international strengths to eradicate the disease from the planet. This Editorial highlights recent research articles and reviews t...
Iqra Mahmood1, Asif Nadeem1*, Masroor Ellahi Babar2, Muhammad Muddassir Ali1, Maryam Javed1, Aisha Siddiqa1, Tanveer Hussain2 and Muhammad Tariq Pervez2
...ne of the most important diseases causing economic losses to dairy farm owners. In order to develop new strategies to prevent mastitis, a detailed understanding of the molecular mechanisms underlying the host immune response to the infectious agents is necessary. Identification of the genes, and their variants, involved in innate immune responses is essential for the understanding of this inflammatory disease and to identify...
Manzoor Hussain*, Miloslav Zouhar and Pavel Ryšánek
Adedeji A.J, Sati N.M, Pewan S.B, Ogbu K.I, Adole J.A, Lazarus D.D, Ijiwo S.J, Okpanachi A, Nwagbo I.O, Joannis, T.M and Abdu P.A
...A) and infectious bursal disease (IBD) and co-infection of CIA and IBD. Infectious bursal disease was tentatively diagnosed in 5,000, 5 weeks old Hy-Line and ISA Brown pullets in a commercial poultry farm in Jos, Plateau State, Nigeria with mortality of up to 79.12%. The clinical signs observed were prostration, ruffled feathers, diarrhea, anorexia, trembling and mortality that lasted for 6 days. Postmortem findings include ...
Jose M. Rojas, Noemi Sevilla and Veronica Martin
...t of a highly contagious disease that affects domestic and wild small ruminants, Peste des petits ruminants (PPR). Due to the important economical losses caused by this virus in Africa and Asia, the development of more effective, targeted and successful treatments for the disease are necessary. Like other Morbilliviruses, PPRV induces a suppression of the immune system during the acute disease...
Mostafa A. Abdel-Maksoud1,2, Fathy A. Abdel-Ghaffar2, Azza El-Amir2, Gamal Badr3 and Saleh Al-Quraishy1*
...s (SLE) is an autoimmune disease that affects many body organs including spleen. However, the effect of Plasmodium chabaudi infection on the spleen of the murine lupus model BWF1with the comparison between live and gamma irradiated parasite was not studied before. A total of 30 female BWF1 mice were randomly divided into three groups as follows: group (I) control group (lupus uninfected); group (II) live P. chabaudi infected group (lupus + live P. chabaudi inf...
Abdul Sattar Baloch1*, Abdul Rasheed1,2, Rahmatullah Rind1, Jam Kashif Sahito1, Rehana Buriro1, Muhammad Faisal Ayoob1 and Parkash Dewani1
...e antibodies against the disease, however, none of the sample was found to be positive for brucellosis. It was concluded that the brucellosis is prevalent among camels of studied areas....
Hussain Ahmad1, Inamullah1, Ijaz Ali2, Tauseef Ahmad3, Muhammad Tufail4, Kabir Ahmad5 and Bibi Nazia Murtaza3,6*
...rucellosis is a zoonotic disease affecting several animal species and human beings. It is caused by Brucella abortus in animals and Brucella melitensis in human. The current study aimed to find out the prevalence of Brucella and active brucellosis in the human population of district Swat. Total 300 individuals (both female and male of all ages, meeting our inclusion/exclusion criteria) with informed consent were included in the study. Blood samples were collec...
Oladipo Elijah Kolawole, Oloke Julius Kola and Awoyelu Hilda Elukunbi
...dditional EBV-associated diseases may occur and some forms of malignancies. This study was designed to determine the incidence of EBV in Human immune-deficient virus (HIV)-infected individuals in Ogbomoso, Oyo State, Nigeria. Two hundred and seventy eight HIV-infected individuals were screened for EBV antibodies over a period of four months (August to November, 2014). The sera of individuals were subjected to serological assay for IgM using enzyme-linked immun...
KM Fakhrul-Islam, Mohammad Shah Jalal, Sonnet Poddr, Md Nurul Quader, Md Sahidur-Rahman, Avijit Dutta and Shuvo Mazumder
...0%....
Jixue Hou1,2, Xueling Chen3, Jing Wang4, Hongwei Zhang2, Yu Xi2, Jie Xia2, Xinyu Peng2,* and Xiangwei Wu2,5*
...rocess of cardiovascular disease, but for a long time it is just limited to large animals, like pigs, rabbits, and big rats. The mice with reproduction fast cycle, lower costing, and close to human genes, can be showed about some phenomenon quickly, also more easy to form model of atherosclerosis. Thus, a mouse model of vascular injury is vitally important for researching the pathophysiological mechanisms to restenosis after percutaneous transluminal coronary ...
Xiangxing Zhu1, Junyu Nie1, Shouneng Quan1,2, Huiyan Xu1, Xiaogan Yang1, Yangqing Lu1, Kehuan Lu1 and Shengsheng Lu1*
...phenotypes seen in human diseases. On the contrary, pigs, especially mini-pigs, show exciting potential for modeling human CNS diseases due to similarities in body size, anatomy, life span, CNS structure and neurobiology. Previously, via the somatic cell nuclear transfer technique, we successfully generated transgenic Guangxi Bama mini-pigs carrying a fluorescent protein (DsRed) reporter gene regulated by the 2.2-kb human gl...
Aziz-ul-Rahman, Farooq Yousaf, Naveed Anwar and Muhammad Abubakar
...n economically important disease of small ruminants that causes extensive livestock losses across areas where it is endemic. A serological survey on the prevalence of antibodies to PPRV was carried out in sheep and goats of Jatoi and surrounding areas in Muzaffargarh districts South Punjab of Pakistan. A total of 965 serum samples were collected from sheep and goat’s farms from January to December 2015 with no history of vaccination. Haemagglutination in...
Ahmed Zein Mahmoud, Muaz Abdellatif and Ahmed Abdalla
...uses a highly contagious disease in both domestic, wild ruminants and camels. Sera from non-vaccinated sheep (n=683), goats (n=624) and camels (n=155) of all ages and sexes were collected in a cross-sectional study in Hail, Bagaa, Shenan and Ghazalah. Saudi Arabia. The seroprevalece was determined by NP-epitopes based competitive ELISA. The overall prevalence was 59.9%, goats had a significantly higher sero-prevalence of 75.3% compared to 59.4% obtained from s...
Sameera Akhtar1*, Muhammad Akram Muneer1, Khushi Muhammad1, Muhammad Yasin Tipu1, Muhammad Anees2, Imran Rashid1, Raza-ur-Rehman3 and Irshad Hussain1
...l measures for Newcastle disease virus (NDV) requires continuous disease surveillance and understanding of field NDV strains. The NDVs, a prototype of Avian Paramyxoviruses (APMV-1), have been reported previously both in chicken and feral birds (natural reservoirs) in Pakistan; however, their molecular characterization is largely inadequate and requires continuous evaluation. In this study, sequencing and molecular analysis ...
Gabriela Mansano do Nascimento, Helena Lage Ferreira and Clarice Weis Arns
...tify;">Infectious bursal disease virus (IBDV) belongs to the genus Avibirnavirus, a dsRNA virus that causes an acute, highly contagious and immunosuppressive disease in young chickens. The first line of defence against the virus is innate immunity whose activation is independent of the antigen and depends on the ability of the host to recognize pathogens by means of specific pattern recognition receptors. This interaction in...
Pam D Luka, Frank N Mwiine, Bitrus Yakubu, Joseph Erume, Ricardo Pérez-Sánchez, Hermann Unger and David Shamaki
...F) is a contagious viral disease of pigs with severe consequences and a growing transboundary potential. The disease is caused by ASF virus (ASFV), the only DNA virus with an arthropod vector of the genus Ornithodoros, involved in its transmission. Tick involvement has been reported in Europe, East and Southern Africa to be part of ASFV transmission cycles. Ticks in an ASFV infected area have been reported to influence the e...
Jehangir Khan1,2*, Inamullah Khan3, Ijaz Ali2, Aqib Iqbal2 and Muhammad Salman4
...res in Shuba Bazaar. The disease burden in Pakistan is critical requiring large-scale molecular epidemiology and entomological surveillance. Moreover, transportation of mosquitoes via tyre trade necessitates more conservative mosquitoes control strategies in Pakistan especially in the unrestricted transport of tyres from southern part of the country to the northern parts.
...
Muhammad Luqman Sohail1,*, Muhammad Sarwar Khan1, Muhammad Ijaz1, Muhammad Avais1, Muhammad Yasir Zahoor2, Omer Naseer1 and Muhammad Usman Saleem3
...globally spread zoonotic disease effecting a wide range of animal species and humans. 18 Leptospira positive horses based on ELISA and 9 healthy horses were included in the study. Blood profile, serum biochemistry and mineral profile were analyzed at day 0, 7 and 21 of infection. Results showed significant decrease (P<0.05) in red blood cells (RBC), packed cell volume (PCV), hemoglobin (Hb), mean corpuscular hemoglobin concentration (MCHC) and platel...
Waqar Islam*1, 2, Madiha Zaynab3, Muhammad Qasim2 and Zujian Wu1,2
...earch findings regarding disease resistance against viral diseases and concludes via shedding light upon the future prospects in this exciting field of research.
...
K.N. ArulJothi1, M. Abinaya1, B. Suruthi Abirami1, Melvin George2, S. Elangovan3 and A. Devi1*
...opment of cardiovascular disease (CVD) in different populations. In this study, we investigated the association of rs5888 polymorphism with serum lipid levels and CVD risk in an Indian population. A total of 412 samples which included 148 myocardial infarction survivors, 162 Normolipidemic healthy controls and 102 patients with hypercholesterolemia in a Indian Tamilian population were included in the study. Genotyping of SCARB1 genetic polymorphi...
Akbar Ali1,*, Khalil Mohamed2 and Fawzia Toulah3
...stify;">Toxoplasmosis, a disease caused by Toxoplasma gondii parasite, is one of the most worldwide parasitic infections of warm-blooded animals including man. In most adults it does not cause serious illness, but it can cause serious health problems in infants, pregnant women and immune compromised patients. Abortion is one of the most serious outcomes in pregnant women. In this cross-sectional study, a total of 162 women of child bearing age were incl...
Tuli Dey, Sonnet Poddar and Mukti Barua
...d important reproductive disease of dairy cattle. A crossbreed cow of 3rd lactation came to Madras veterinary college with the clinical sign of complete uterine prolapse which calved one day before. The cow was handled and the prolapsed mass was corrected so carefully that there could be saved the life without any complication. The prolapsed mass of cow was managed with lukewarm water and manual pressure replacement in non-raising condition of hindquarter. Vul...
Erum Bughio1, Ahmed Sultan Jatoi*1, Muzamil Memon2, Reema Bughio3, Pervez Ahmed Khoso4, Zafar Ali Khoso1 and Ali Asghar Baloch5
Irum, Akram Shah*, Sobia Wahid, Nazma Habib Khan and Qaisar Jamal
...nculiasis or guinea worm disease causes severe morbidity and disability among effected populations. Pakistan was declared dracunculaisis-free in 1996. The present study is a follow-on to the declared eradication of dracunculiasis in Dera Ismail Khan district of Khyber Pakhtunkhwa (KP) Province, Pakistan through investigating disease recurrences and Cyclops species in drinking water sources. A cross-sectional household...
Syed Ishfaq Ali Shah1,*, Tassawar Hussain Malik2, Imran Rafi Khan1 and Zahid Hussain3
...f curl virus (CLCuV) disease with promising local varieties tolerant to the disease. After screening, the most resistant accessions of USDA were USG-14-2464 and USG-14-2478. However, USG-14-2481 and USG-14-2484 were recorded as partially resistant to CLCuV disease. Among the local germplasms, all accessions screened were found extremely susceptible. Disease
Yi-qiang Ouyang1, Zhen-xin Liang1, Shi-wen Huang2, Ying Zhang3, Shao-shi Luo1, Jin-ning Liang1, Jia-fu Li1, Yu He4 and Song-chao Guo1,*
...ies of Alzheimer’s disease (AD) involve rats and mice, yet the tree shrew (Tupaia belangeri) is in many respects more similar to primates than rodents are. To demonstrate the feasibility and usefulness of using the tree shrew as an AD model, we examined how well the model recapitulates features of the disease. Injecting animals intraperitoneally with d-galactose to induce acute senility, and then injecting them ...
Zia Ur Rahman1*, Syed Mehar Ali Shah1, Hidayat Ur Rahman1, Fida Mohammad1, Mian Ahmad Raza1 and Ijaz Ahmad Khan2
...st bacterial leaf blight disease at the University of Agriculture, Peshawar-Pakistan during 2011 rice crop growing season. On artificial inoculation with Xanthomonas oryzae pv. Oryzae, the fundamental agent of bacterial leaf blight, most of the studied rice accessions and the two commercial rice cultivars displayed susceptible reactions. Seven rice accessions viz. UoA-2, UoA-5, UoA-11, UoA-48, UoA-53, UoA-102, and UoA-123 showed moderate levels of resistance w...
Antibacterial Activity by Chlorogenic Acid Isolated through Resin from Apricot (Prunus Armeniaca L.)
Ahmed Mujtaba1, Tariq Masud1, Asif Ahmad1, Waqar Ahmed3, Saqib Jabbar3* and Robert E. Levin2
...pplemental treatment for diseases associated with these pathogens.
... 
Nirbhay Kushwaha, Achuit K Singh, Brotati Chattopadhyay and Supriya Chakraborty
Recent advances in geminivirus detection and future perspectives
... their vectors, menacing disease epidemics caused by newly emerging or reemerging begomoviruses are becoming frequent and appearing even in new regions, previously free from such diseases. Techniques for geminivirus detection include biological indexing, electron microscopy, antibody-based detection, including ELISA, nucleic acid hybridizations like dot blot, squash blot and Southern blot techniques, polymerase chain reactio...
M. B. Meah
Biopesticides for crop growth and crop protection
...ly controlled soil-borne diseases including root-knot nematode. Mahogany oil spray reduced infestation of stem borer,leaf hoppers of rice; shoot & fruit borer of brinjal and fruit flies of vegetables.Biopesticides (formulated trichoderma) and bio-agents (bracon,trichogramma, ladybird beetle) and sex pheromone are in the pipeline for commercial use....
A. K.Bhattacherjee and B. K. Pandey
Dissipation of carbendazim in mango after pre- and post-harvest treatments
... to control post harvest diseases viz., anthracnose (Colletotrichum gloeosporioides) and stem end rot (Lasiodiplodia theobromae). Carbendazim dissipated to 1.01 and 2.14 mg kg in mature whole fruit after harvest (12 days after second spray) at 0.05 and 0.1 per cent concentrations, respectively, following first order rate kinetics. The corresponding values in fruit pulp after 12 days were 0.57 and 1.26 mg kg . In another experiment, cold water dip treatment in ...
M. D. Maji, N.K.Das, S. Chatterjee, A. Ghosh and A.K. Bajpai
Forecasting models of bacterial leaf spot disease of mulberry for Birbhum district of West Bengal
... one of the major foliar diseases in mulberry at farmers field in Birbhum district.BLS incidence appeared in May and continued up to November. The correlation coefficient between disease severity and meteorological parameters revealed that BLS disease severity showed significant positive correlation with max & min temperatures, min relative humidity, rainfall and number of rainy days. ...
B. Ramanujam, R. D. Prasad, S. Sriram and R. Rangeswaran
Mass production, formulation, quality control and delivery of Trichoderma for plant disease management
...ol of several soil-borne diseases of some field crops. Similarly, seedlings of horticultural crops and rice are treated by dipping the roots in Trichoderma suspensions before planting. Granular or pellets preparations and Trichoderma enriched FYM have been used for soil application directly and have provided effective control of diseases both nurseries and field conditions. To ensure that the products of Trichoderma do not a...
A. K. Chaubey and Satyandra Kumar
Bio-management of root knot nematode and root rot disease by antagonistic fungi and rhizobacteria
...n root knot and root rot disease and promote plants growth as the length and weight of root and shoot in pot trials on tomato plants. Keywords: Antagonistic fungi, root knot nematode, wilt fungus, bio-management
...
Sitansu Pan and N. K. Mishra
Epidemiological studies on some diseases of guava (Psidium guajava L.)
...ty on the development of diseases like canker (Pestalotia psidii), anthracnose and die back (Colletotrichum gloeosporioides), stem canker and dry fruit rot (Botryodiplodia theobromae) and Phytophtora fruit rot (Phytophthora nicotianae var parasitica) of guava were analysed for predictive purpose from regression equations. The simple correlation coefficient matrix showed significantly positive correlation of canker severity with maximum relative humidity at 1% ...
Bijan Kumar Das
Incidence of Aleurocanthus spp. (Aleyrodidae: Hemiptera) on betelvine (Piper betle L.) and their interaction with host plants
...ity to several pests and diseases, aggravated by the nature of the plantation. A good number of hemipteran insect pests occur in betelvine ecosystem which dwindle betelvine yield potentiality. Among these, the polyphagous betelvine blackfly, Aleurocanthus rugosa Singh (Aleyrodidae: Hemiptera) is a major pest causing severe damage to the foliage in the conservatories (borojas) of West Bengal. The seasonal incidence of A. rugosa in boroja was recorded during 200...
Bushra Afreen, Geetesh Baghel, Akil A. Khan and Q.A. Naqvi
Virus- vector relationship of yellow vein disease of calendula and the whitefly
...irus causing yellow vein disease on Calendula officinalis L. was transmitted from naturally infected C. officinalis to healthy seedlings of C. officinalis through whiteflies not through sap inoculation. A single whitefly could transmit the virus and showed 20.55% infection and fifteen whiteflies were required for 100 percent transmission. The minimum acquisition access feeding period and minimum inoculation access feeding period for the present virus were 10mi...
S. K. Dutta, S. Roy Chowdhuri, D. Pandit and A. K. Bajpai
Rot diseases of muga host, Som (Persea bombycina) in Assam, India
...erally infected with rot disease when it grows older. Phellinus contigus (under Polyporaceae), a white rot fungus, causes heart rot disease and Biscogniauxia mediterranea (= Hypoxylon mediterranea) (under Xylariaceae) causes canker rot disease of Som plant. A survey was conducted to study the infection of rotting pathogens on Som plants at Regional Muga Research Station (RMRS), Boko, Assam...
Sitansu Pan and Someshwar Bhagat
Biological control of plant diseases
...logical control of plant diseases seems to be the best alternative for plant disease control. It is ecofriendly, reliable, cost effective and is an integral component of integrated plant disease management framework for sustainable crop production. A considerable number of biocontrol agents have been established successfully for their bioconrol potential including strains belonging to bact...
Basudeb Dasgupta, Partha Dutta and Srikanta Das
Biological control of foot rot of betelvine (Piper betle L.) caused by Phytophthora parasitica Dastur
...ed that minimum foot rot disease occurred where four drenching and eight sprayings of BM at monthly and fortnightly intervals respectively were applied in the 1st year. In the 2nd year, the minimum foot rot disease was recorded in the treatment where four applications of the Trichoderma preparation ha-1 at quarterly intervals were given. The maximum foot rot disease was recorded in control...
Debjani Chowdhury, P. C. Paul and B. Dasgupta
Management of leaf spot of Centella asiatica (Thankuni) caused by Alternaria sp. and target leaf spot of Rauvolfia serpentina (Sarpagandha) caused by Corynespora cassicola
... that the lowest percent disease incidence (PDI) of leaf spot of Thankuni caused by Alternaria sp. and highest yield in number of leaves per sq.M was recorded where carbendazim was sprayed. The highest yield in fresh weight, dry weight of leaves, fresh weight and dry weight of 1000 leaves was recorded. Application of mancozeb resulted in the lowest PDI of target spot of Sarpagandha, increased the yield (both fresh weight and dry weight of the plant per sq.M) a...
M. S. A. Mamun and M. Ahmed
Integrated pest management in tea: prospects and future strategies in Bangladesh
...sects, mites, nematodes, diseases and weeds. Globally 1034 species of arthropods and 82 species of nematodes are associated with tea plants. Among them 25 species of insects, 4 species of mites and 10 species of nematodes are recorded from Bangladesh. Enormous crop loss was incurred due to the attack of these pests and largely responsible for the declining productivity of tea. Extensive use of chemical pesticides began only a few decades ago with tremendous im...
Sitansu Pan and Amrita Das
Control of cowpea (Vigna sinensis) root and collar rot (Rhizoctonia solani) with some organic formulations of Trichoderma harzianum under field condition
...suffer from a variety of diseases. Among those root rot and collar rot are important and R. solani has been an important factor behind these two main diseases. A field trial was conducted during pre-kharif season of 2011 to control root and collar rot of cowpea caused by Rhizoctonia solani with two organic formulations of Trichoderma harzianum along with other treatment combinations. It was observed that seed priming with my...
Sunita J. Magar and D.D. Nirmal
Effect of plant spacings and cultivars on spread of Yellow Vein Mosaic Virus (YVMV) disease
...vein mosaic virus (YVMV) disease in different plant spacing 30 x 30 cm, 50 x 30 cm, 60 x 30 cm, 60 x 60 cm and 60 x 40 cm and using two cultivars, i.e., cv. Parbhani Kranti and Local indicated that the first appearance of symptom was earlier in cv. Local and late in cv. Parbhani Kranti. The rate of infection was higher at 45 to 60 days after sowing in both kharif and summer while in cv. Local it was higher at 30 to 45 days after sowing. Less infection rate was...
P.S. Ajith, K.K. Lakshmesha, S. Mahadev Murthy and N. Lakshmidevi
Botanicals for control of anthracnose of bell peppers
...ponsible for anthracnose disease in bell peppers (Capsicum frutescence L.). The results showed that all the selected plants have potential to inhibit the radial mycelial growth of C. capsici in-vitro. The plant extract from C. aromaticus at 50% concentration showed 42% radial mycelial growth inhibition in poisoned food technique, whereas 15% increased seed germination and 26% reduced disease incidence is recoded when compare...
Goutam Mondal
Plant growth promoting activity of some indigenous Tricho-derma isolates and their field performance against sheath blight of rice in old alluvial zone of North Bengal
...educe rice sheath blight disease incidence (46.32% to 70.54%) over check plot significantly. Again, the efficacy of the biocontrol agents was significantly greater in the block with green manured than the block without green manured. Application of hexaconazol 5% EC@ 2.0 ml litre-1 and carbendazim 50% WP @ 1.0 ml litre-1 also reduced the sheath blight disease incidence by 53.25% and 56.86% over the check plot respectively. I...
M.S. Islam, M.Ali, and I. Ahmad
Efficacy of sedomil 72WP and recozeb 80WP to control die-back (Colletotrichum gloeosporioides) of tea in Bangladesh
...l) to control of dieback disease (Colletotrichum gloeosporioides) of tea in Bangladesh. The pathogen, C. gloeosporioides was isolated from the dieback infected tea twigs on PDA medium. Mycelial blocks of the isolated pathogen were transferred to PDA plates amended with sedomil 72WP (mancozeb) @ 720, 1080, 1440 and 1800 ppm and recozeb 80WP (mancozeb + metalaxyl) @ 800, 1200, 1600 and 2000 ppm concentrations. Sedomil 72WP and recozeb 80WP caused colony growth i...
A. K. Ganguly and Uma Rao
Decades of researches in biochemical and molecular nematology at IARI
...ockle nematode and molya disease of wheat. Currently attempts are being made to utilize RNAi technology to identify the genes targets for the management of root knot and cyst nematodes. In this paper some of the success stories of the technology developed by the scientists at IARI are discussed.
...
Partha Pratim Ghosh and 2N. C. Mandal
Some disease management practices for bacterial wilt of potato
...f the threatening potato diseases. To study the effect of some disease management practices in potato, an investigation was conducted in slightly acidic (pH 5.62) loamy sand soil with medium fertility status in a Randomised Block Design with three replicates and seven treatment modules, as described in Materials & Methods. Investigation revealed that T1 (TPS whole tuber planting) and T4 (supervised management - cowdung @...
T. P. Ghosh, D. Mandal, S. Laha and M. K. Dasgupta
Dynamics and severity model in managing fungal diseases
...dia, to study the fungal diseases of some commercial ornamentals. The leaf spot of Ficus religiosa (c.o. Alternaria sp.) is the first record. Fifteen fungal diseases have been formally described from West Bengal for the first time on these twelve ornamentals. Monthly dynamics was determined for occurrence, intensity and severity of these diseases. The disease
Shyam Singh and L.P. Awasthi
Plant products for the management of yellow mosaic disease of mungbean and urdbean
...an. Maximum reduction in disease incidence recorded were 66.70 and 63.65 percent in mungbean and Urdbean, respectively by eight sprays of Clerodendrum aculeatum. Whereas, eight sprays of Boerhaavia diffusa root extract could reduce the disease incidence by 60.27 and 58.20 per cent followed by Azadirachta indica leaf extract by 42.43 and 42.92 percent in mungbean and urdbean, respectively. Maximum plant height (70.90 and 56.2...
Beenish Zahid1,*, Asim Aslam2, Zafar Iqbal Chaudhry2 and Raheela Akhtar2
... field infectious bursal disease virus (IBDV). The Bursa of fabricius was collected from poultry flocks of Punjab Pakistan for isolation of IBD virus by inoculating in embryonated chicken eggs through chorioallantoic membrane. The two hundred birds were divided into 4 groups A, B, C and D). The birds in group A were commercial broiler chicks having maternal derived antibodies and were challenged with IBDV field isolate at the rate of 0.1ml of EID50 (virus tite...
Muhammad Mansoor, Zaigham Abbas and Nageen Husssain*
... is a chronic autoimmune disease with characteristic organ involvement and autoantibodies production. The pathogenicity and etiology of the disease has yet to be elucidated. It is presently accepted that environmental factors trigger the disease in genetically sensitive individuals. Gluten, a protein fraction commonly found in wheat grains, associated with food related disorders and a numb...
P.P. Ghosh*, C. Ghosh, B. Mahato, A. Chakraborty, S.K. Bhattacharya and M.K. Bhattacharjya
...oting and destruction of diseased plants (farmers’ practice). Results indicated all the management approaches under study performed significantly superior to farmers’ practice in terms of reduction in disease severity (32.8-43.6%), yield increase (20.9% - 26.8%) and benefit-cost ratio. Integrated approach was the best approach followed by prophylactic and curative soil disinfestations. Thus, for the present micro...
Mahmoud Said1*, Abd El Satar Arafa1, Mohammed A. Rohaim2* and Hussein A. Hussein2
...ign: justify;">Newcastle disease virus (NDV) and avian influenza viruses (AIVs) H5N1 are endemic in Egypt and extensive vaccinations are being applied for chicken flocks, which have maternal derived antibodies (MDA) for these viruses. To understand the interference of maternal derived immunity impact of maternal derived immunity interference in vaccination regimen in poultry, a total of one hundred day old commercial broiler chicks were vaccinated with LaSota....
Hussein Aly Hussein1*, Omneya Mohamed Khattab2, Shereen Mohamed Aly2, and Mohammed Abdel Mohsen Rohaim1
...ansmission of lumpy skin disease (LSD), which is an economically important disease of cattle and is caused by the LSD virus (LSDV). LSD is endemic in most countries of Africa and Middle East and can be transmitted either by mechanical as well as intrastadial and transstadial routes. Since capripoxviruses are serologically identical, their specific identification relies exclusively on the use of molecular tools. In this study...
Naimat Ullah Khan1,2, Muhammad Hassan Saleem1, Aneela Zameer Durrani1, Nisar Ahmad3, Ayesha Hassan1, Sultan Ayaz2, Sajid Umar4, Muhammad Luqman Sohail5,*, Muhammad Shafee6, Ikramullah Khan7, Mumtaz Ali Khan1, Azmat Ullah Khan8, Naimat Ullah Malik1 and Abdul Razzaq9
... age groups with highest disease prevalence in children of less than 5 years of age (21.40%) and lowest in children of 11-15 years of age (15.99%). Cryptosporidium was significantly higher (P<0.05) in study subjects having livestock animal contact and persistent diarrhea, highlighting its zoonotic potential from livestock animals to human beings in the study area. This is first study addressing the one of the main cause of neonatal mortality in South...
Viram Kumar1,*, Moolchand Malhi1, Saeed Ahmed Soomro1, Toufique Ahmed Qureshi2, Muhammad Nawaz Sanjrani3 and Khushal Das Malhi1
...o evaluate the health or diseased conditions in the same species.
...
Javeria1, Masroor Ellahi Babar2,*, Tanveer Hussain2, Rashid Saif2, Sadaf Rashid3, Hanan Sarfraz4, Abbas Ali Shah3, Muhammad Wasim1 and Muhammad Abdullah1
...d to detect the possible disease causing effect of genetic variants with OCD. Sequencing of BDNF gene showed one change G>A at position c.196 (rs6265) resulting in a nonsynonymous change from valine to methionine. We found only one homozygous and heterozygous carrier for this polymorphism. Healthy siblings of the carrier individuals and control group were screened and found as negative for this polymorphism. In-silico analysis through mutation taster...
Madiha Hashmi1, Abid Hussain1, Shafiq ur Rehman1, Farida Ahmed2, Shahbaz Aslam3,Nadeem Afzal4 and Zaigham Abbas1,*
...ce of different allergic diseases in Pakistan is about 66.1% and every year, almost 2.4 million people get vaccine for allergy from Allergy Centre, National Institute of Health (NIH), Islamabad. Human Leukocyte Antigens (HLA) genes are located on the short arm of the chromosome number 6 and position 21 and the allele frequency is associated with allergy. The study was conducted to investigate the relationship between the aero-allergens and the frequency...
A. Hamid1,*, M. Ilyas2 and S. Kalsoom3
...eading to cardiovascular diseases are very common problem in Pakistan and in most cases it is caused due to insufficient intake of dietary fiber.A study was conducted to evaluate the role of dietary fiber and ß-glucan from major food grains used locally on plasma cholesterol level of dietary-induced hypercholesterolemic rats. Insoluble dietary fiber (lignin and cellulose) was extracted from wheat and maize, while ß glucan was extracted from barley ...
Wesam Hasan Mohamed Mady1*, Bing Liu2, Dong Huang2, Abdel Satar Arafa1, Mohamed Khalifa Hassan1, Mona Mehrez Aly1, Pucheng Chen2, Yongping Jiang2* and Hualan Chen2
... treatment of many viral diseases because of its ability to induce both humoral and cellular immune responses against antigens encoded by recombinant DNA. The goal of this study was to construct Egy-H5 plasmid DNA-based vaccine against a currently circulating avian influenza virus H5N1 strain in Egypt targeting the HA gene. The HA gene of AIV Egyptian strain H5N1 was codon optimized for chicken biased codon and sub-cloned into pCAGGS mammalian expression vecto...
Ahmed Zein Elabdeen Mahmoud1, Muaz Magzob Abdellatif2* and Mohamed Abdelsalam Abdalla3
...minants (PPR) is a viral disease which primarily affects small ruminants, causing significant economic losses for the livestock industry. Clinical investigation was carried out during the suspected outbreaks of PPR/FMD in sheep and goats in Hail. Saudi Arabia. During the outbreaks 271 (20.6%) died out of 648 (49.3%) of affected animals, the overall morbidity in goats (33.3%) was higher as compared to sheep (12.3%), with a case fatality rate in goats reached 74...
Mehran Kausar1,2, Naveed Ashraf3, Farzana Hayat4, Asraf Hussain Hashmi1, Saima Siddiqi1,* and Mariam Anees2
... k) responsible for this disease. Mutations in CTSK result in impaired bone resorption consequently leading to short stature, increased bone density, recurrent fractures, stubby hands and feet with dystrophic nails, unossified fontanels, and an obtuse mandibular. The present study was conducted to determine the underlying genetic cause of Pycnodysostosis in a family from Azad Kashmir, Pakistan presenting with symptoms of multiple recurrent fractures, sh...
Muhammad Ibrahim1,2*, Syed Jawad Ahmad Shah1, Shaukat Hussain2, Musharaf Ahmad2 and Farhatullah3
...ne of the most important diseases of wheat in many parts of the world including Pakistan. Surveillance for yellow rust occurrence, distribution and impact on wheat landscape was carried out along with recent deployment status of cultivars during 2012 in northwest Pakistan located close to the center of diversity of P. striiformis f.sp. tritici. Around 1600 wheat fields were sampled covering a geographical area of little less than 5000 acres in the southern, ce...
Muhammad Arslan Khan1,*, Sajid Aleem Khan1, Imran-ul-haq1 and Rashad Waseem Khan2
...Root rot is very drastic disease prevalent in major cotton producing areas of Pakistan. Complex nature of disease due to root rot fungi and root-knot nematode cause huge losses to cotton. Experiments were conducted to assess the role of fungi and root knot nematodes in root rot disease complex of cotton and to optimize their control. For this purpose fifteen cotton growing areas in Souther...
Javeria1, Masroor Ellahi Babar1,2,*, Akhtar Ali2, Asif Nadeem1, Abdul Wajid1, Sajjad Ali Shah3, Sadaf Rashid4, Muhammad Wasim1 and Muhammad Abdullah1
...ed it to be damaging and disease causing. Screening of case and control group data showed no deviation from Hardy-Weinberg equilibrium with p-value 0.879 and 0.32, respectively. There was no significant difference in age (p-value 0.081), employment status (p-value 0.34) and matrimonial status (p-value 0.28) but there was a significant difference in their education (p-value 0.0002). The prevalence of C allele was 13.5% in
Carolina Torres Alejo1,2, Laila Andreia Rodrigues Beserra1,2, Paulo Eduardo Brandão1,2, Luis Ramiro Luna Espinoza1,2 and Fabio Gregori1,2*
... associated with enteric diseases in avian species, causing decreased growth performance and mortality. In this study, 59 pools of intestinal content of layers, broilers, and breeders were investigated from different Brazilian regions for the presence of rotavirus (RVA, RVD, and RVF), avian coronavirus (ACoV), and avian astrovirus (AAstV), using PCR reactions followed by nucleotide sequencing and analysis. Twenty-nine pools (29/59; 49%) appeared positive for A...
Mohsan Ullah Goraya1,2, Liaqat Ali1,2 and Iqra Younis3
...eir impact in health and disease. ...
Lucas Pedroso Colvero1, Paulo Eduardo Brandão2*, Cesar Alejandro Rodriguez Rosales2, Hanny Swam3 and Laura Yaneth Buitrago Villarreal1
...ly used to control viral diseases in poultry, however, the interference of physico-chemical properties of the emulsions are overlooked when compared to antigenic properties. This study was designed to assess the effect of two different commercial inactivated water-in-oil multivalent vaccines; vaccine A contains IBV (Massachusetts M41 and D274 strains), NDV (Clone 30), aMPV (BUT 8544) and IBDV (classic strain) whereas vaccine B contains IBV (Massachusetts M41 a...
Zia-ur-Rehaman1, Naila Chand1, Sarzamin Khan1 and Rifat Ullah Khan2,*
... titer against Newcastle disease (ND) and serum paraoxonase (PON1) were significantly higher (P< 0.05) in TN zone, while serum malondialdehyde (MDA) was significantly (P< 0.05) higher in HAT zone. No significant differences were recorded in serum antibody titer against ND, PON1 and MDA among different broiler strains in TN zone, while in HAT zone, Ross and Arber Acer showed significantly (P<0.05) higher serum antibody titer against ND. In HAT zone, si...
Faiz Muhammad1,*, Syed Khurram Fareed1, Urooj Zafar1, Taseer Ahmed Khan2 and Aqeel Ahmad1
...economically significant disease in commercial poultry industry. This study aimed to develop and optimized a novel tPCR approach to detect Mycoplasma gallispeticum, Mycoplasma synoviae and nonpathogenic mycoplasmas at the same in a single PCR reaction collected from diseased chicken. Direct PCR from the clinical samples produced false negative results. Both culture and PCR were combined as culture-enhanced PCR ...
Zhenyu Chang1, Hui Zhang1, Khalid Mehmood1,2, Mujeeb Ur Rehman1, Xiaodan Yuan1, Zhaoyang Li1, Fazul Nabi1, Xiaoxing Wu1, Xinxin Tian1, Xueting Liu1, Jingen Xu3 and Donghai Zhou1,*
...tor (TFF3) in intestinal disease was explored. Porcine intestinal epithelial cells were treated with lipopolysaccharide (LPS) and recombinant human (rh) TFF3. Cell proliferation and TFF3 mRNA expression were examined by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assays and real-time quantitative PCR, respectively. The results showed that rhTFF3 effectively prevented the cell injury induced by LPS (P<0.05). A low concentratio...
Jing Xin Mao1,2, Guo Wei Wang2, Yuan She Huang3, Rui Wang2, Guo Ze Wang4, Bing Zeng1 and Fu Yuan Zuo1,*
...s a better resistance to disease than Landrace. When Rongchang pig and Landrace were 0 day old, the expression level of BPI of Rongchang pig was higher than Landrace in heart, liver, spleen, kidney, small intestine, lower than Landrace in lung. When Rongchang pig and Landrace were 28 day old, the expression level of BPI of Rongchang pig was higher than Landrace in spleen (p<0.05), kidney (p<0.05), small intestine (p<0.05); lower t...
Pei-feng Yin1,2,Xiu-xiu Li1,Qi-wei Zeng3, Cheng-chen Shen1, Le Gao1 andJian-zhang Ton2,*
...berry.
...
Imtiaz Ahmed, Muhammad Abbas Khan, Noorullah Khan, Naveed Ahmed, Abdul Waheed, Fazal Yazdan Saleem, Sajjad Khan and Sohail Aslam
...he study. Data regarding disease occurrence, disease severity, plant height (cm), bulb weight (g), bulb diameter (mm), clove weight (g), number of cloves bulb-1 and yield (t ha-1) was measured. The result of the study reveals that plant spacing had significant impact on the occurrence of rust disease and its severity, yield and yield components. Plants with 10 cm spacing had more
Gut Microbe Analysis between Asthma Patients and Healthy Volunteers in Shaanxi Province, Xian, China
Hafiz Muhammad Ishaq1, Muhammad Shahzad2, Xiaokang Wu3, Chaofeng Ma4 and Jiru Xu1,*
...llergic and chronic lung disease that causes inflammation and narrows the air passages. Present study aimed to characterize the gut microbiota of asthma patients and to investigate the alteration in similarity and diversity of gut microbial composition with comparison of healthy controls. Fecal samples from fifteen patients and five healthy individuals were collected. PCR-DGGE, by using universal primers focusing V3 region of 16S rRNA gene, was performed for c...
Muhammad Saqlain1, Humaira Kalsoom1, Muhammad Fiaz1,2, Abid Mahmood1, Rizwan Aziz Qazi4 ,Waseem Safdar5, Pakeeza Arzoo Shaiq1,Bernard M.Y. Cheung3 and Ghazala Kaukab Raja1,*
...P2 54Thr allele with disease and provides further evidence for the involvement of intestinal fatty acid binding protein in the pathogenesis of MetS.
... 
Rana Hanan1*, Muhammad Waqar Shafi1, Sumair Anwar2 and Numrah Nawaz1
...a multifactorial chronic disease and a silent cause of high causalities around the globe. Both hyper and pre-hypertension affect all ages and professions, and are as common as flu infections by affecting 1 in every 5 persons. In order to determine frequency of hypertension and pre-hypertension among medical students and their association with various variable and fixed risk factors, this study was designed. The cross sectional study of 4 months was carried out...
Warda Fatima1 , Shahida Hasnain1 , Saqib Mahmood2
...ed one of the most costlydisease in terms of diagnosis and treatment. Its heritability content is considered to be up to 80% but still it isunknown whether it’s a pure genetic disease. It is considered a polygenic multifactorial condition where both genes and environment have roles in disease development. In the present study we selected a family withmultiple schizophrenic patients a...
Amjad Khan1,*, Muhammad Hassan Mushtaq1, Mansur ud Din Ahmad1, Jawad Nazir1, Zahida Fatima2, Asghar Khan3 and Shahid Hussain Farooqi3
...gious equine respiratory diseases. Due to lack of awareness and interest of all the equine industry stakeholders no vaccination against EI is performed, also the information regarding the epidemiology and occurrence of EI is lacking in Pakistan. We hereby attempted to determine the seroprevalence, investigating the EI outbreak 2015-16, and describing the demographic and management risk factors associated with seroprevalence of EIV. Using ELISA kit and HI as se...
Qamar-un-Nisa1, Muhammad Younus2,*, Muti-ur-Rehman1, Azhar Maqbool3 and Sajid Umar4
...t.
...
Tania Ahmed Shakoori1,*, Muhammad Saqib Saeed2, Asif Hanif2, Saima Mukhtar3 and Ayesha Ghauri4
...ic obstructive pulmonary disease (COPD). Their frequency is directly related to the prognosis of the disease. The objective of this study was to identify the ‘frequent exacerbator’ phenotype and determine the risk factors for increased frequency of acute exacerbations in local chronic obstructive pulmonary disease (COPD) patients in a cross sectional analytical study design. Fo...
Muhammad Jamal Nasir*, Anwar Saeed Khan and Said Alam
...t crops, pests and crops diseases, flowering time of fruits trees, biodiversity and livestock’s. Respondents were selected randomly but priority was given to elders and those possess land and practice agriculture. The study reveals that majority of the respondents are well aware of climate change and its effects on cropping calendar, crop variety, crop yield, crop diseases as well as an earlier blossoming of fruit tree...
Tahira Kamal1*, Saeed-Ul-Hassan Khan2, Amir Bin Zahoor3, Khalid Naeem3, Muhammad Naeem Riaz1, Siddra Tayyab Akhtar4, Ghulam Muhammad Ali1*
Muhammad Abubakar,1,2,* Aamer Bin Zahur,1,3 Khalid Naeem1,3, Muhammad Azeem Khan1,3 and Subhan Qureshi4
...economically devastating disease of sheep and goats. Present study was designed to have an insight into the epidemiology of PPR under field conditions in the country using molecular tools. A total of eighty-four (n = 84) PPR outbreaks were investigated during the study (2010 to 2013). The highest number of outbreaks was reported from Punjab province (n = 38) followed by Sindh (n = 21) and Khyber Pakhtunkhwa (KPK, n = 10). In 48 out of 84 outbreaks,
Aqsa Waqar1, Sahir Hameed Khattak2, Sania Begum2*, Tayyaba Rehman1, Rabia1, Armghan Shehzad2, Wajya Ajmal2, Syeda Shahdana Zia2, Iqra Siddiqi2 and Ghulam Muhammad Ali2*
...s a globally significant disease of wheat. Stripe rust was primarily considered a disease of cooler climate (2°C - 15°C), upper altitudes and northern latitudes, but current epidemics of the disease have confronted this supposition because fresh strains have greater adaptation to higher temperatures and countries closer to the equator. Crop damages can reach 50 - 100%, due to infec...
Aneeza Jamshed1* and Syed Amir Gillani2
...61% thought that it is a disease of adults. Only 21.1% students thought it can be cured. In post awareness assessment after 3 sessions of interactive learning, 92% students understood the prevention of hepatitis C disease, its transmission (syringes 88.7%, blood transfusion 91%), signs (effects on liver 92.9%), symptoms (88%), prevention in high prevalent areas (sterilized syringes and screened blood transfusion 86.5%) and m...
Muhammad Yahya Noori1*, Saharish Rizwan2, Zaheer Ali3 and Shaheen Sharafat4
...who are unaware of their disease. ...
Wali Khan1,*, Noor-Un-Nisa2 and Muhammad Asif Nawaz3
Muhammad Naeem1, Bushra Sadia1,2,*, Faisal Saeed Awan1and Muhammad Anjum Zia3
... in treatment of various diseases like ischemia, Budd-Chiari syndrome, myocardial infarction, brain stroke and myocardial infarction. Strain improvement is an important approach for its hyper production. In this connection, wild bacterial strain Streptococcus equisimilis was mutagenized using ethidium bromide and UV radiation. The colonies (15 each) showing > 1% survival rate at 150 min of UV exposure and 12 % survival at 0.5 mg/ml of ethidium bromid...
Sadaf Ghulam Rasul1*, Uzma Yaqub2, Maida Manzoor1 and Hafsa Mubasshar1
...is an estrogen-regulated disease, and is signified by the existence of a particular endometrial tissue exterior to the uterus. It is a principal cause of long lasting morbidity, mostly from serious pelvic pain(1). Endometriotic tissue has the potential for aromatase gene expression that leads to aromatase and estrogen production. Danazol is a synthetic androgen whereas Letrozole is aromatase inhibitor. This study was conducted to compare the mean decrease in v...
Mohammad Siddique Munawar, Shazia Raja, Elizabeth Stephen Waghchoure* and Muhammad Barkat**
...llus larvae is a serious disease of honey bees worldwide that inflicts considerable economic losses to beekeepers. All work was undertaken in a commercial apiary that showed visual signs of AFB infection. Twenty four hives positive for AFB were identified from the apiary (4 colonies in each replicate). Half of the colonies were treated with shook swarm method while other half were left without any treatment. The treated colonies showed significant difference f...
Muhammad Athar Khan1, Muhammad Zahid Latif2*, Syed Amir Gilani3 and Ifrah Bukhari4
...obal zoonotic infectious disease having alarming public health concerns. Generally this is an occupation related disease and the victims belong to animal slaughtering, owners of pet shops, farm workers, handlers of meat, sewerage and agriculture workers. Leptospirosis affect multiple organs in human body and may lead to myocarditis, renal failure, respiratory distress and hypotension. This disease
Iracema Nunes de Barros1,2, Luciane Dubina Pinto3, Rosely Bianca dos Santos Kuroda1, Sheila Oliveira de Souza Silva1, Leonardo José Richtzenhain1,2, Cláudio Wageck Canal3 and Paulo Eduardo Brandão1,2*
...tes to better assess the disease transmission dynamics and future control strategies. ...
Imtiaz Hussain*, Hassnain Shah, M. Azeem Khan, Waqar Akhtar**, Abdul Majid*** and M. Yaqub Mujahid*
Corresponding author:[email protected]
PRODUCTIVITY IN RICE-WHEAT CROP ROTATION OF PUNJAB: AN APPLICATION OF TYPICAL FARM METHODOLOGY
...eased weed problem, more disease and insect invasion leading to yield losses. Berseem is the only crop that breaks this rotation. Farmers are aware of the beneficial effects of this crop rotation and believed that this riceberseem rotation control weeds in the next wheat crop along with yield -1 -1 improvement of both rice (upto 5.4 md acre ) and wheat (4.4 md acre ). However there is need to assess the economic impact and evaluation of berseem in rice-wheat c...
A. N. Naqvi and K. Fatima*
INCIDENCE OF LIVESTOCK DISEASES IN NOMAL AND NALTAR VALLEYS GILGIT, PAKISTAN
...e incidence of livestock diseases in Nomal and Naltar valleys, Gilgit. The data on cattle, goat, sheep and donkey were collected from the Animal Husbandry Department from 2003 to 2007. In total 19259 animals were found affected with various diseases. The disorders reported in the area were digestive diseases, infections, mastitis, reproductive diseases, ...
Muhammad Sharif*, Muhammad Azam Niazi*, Abdul Jabbar** and Sajida Taj***
FINANCIAL PROFITABILITY OF WHITE SUGAR PRODUCTION IN PAKISTAN
... measures are needed for disease control and optimal pesticide use. Adequate supply of water, optimal use of fertilizer and judicious use of pesticides can improve yield and hence higher financial profitability....
Maqsood Qamar*, S. Dilanawaz Ahmad Gardazi**, Riaz-ud-Din*, Muhammad Sohail* and Syed Haider Abbas*
PARTIAL RESISTANCE TO STRIPE RUST AND ITS EFFECT ON SUSTAINABILITY OF WHEAT YIELD
... is prone to stripe rust disease. It can cause 10-17% yield losses if susceptible cultivars are planted under favorable conditions. Level of partial plant resistance in bread wheat and its impact on sustainable wheat production was studied at the National Agricultural Research Centre, Islamabad under natural conditions in the field. Eleven Pakistani commercial wheat cultivars/advance lines including check (Inqalab 91) were assessed for the level of partial res...
Frasat Saeed*, Jehanzeb Farooq*, Abid Mahmood**, Tassawar Hussain***, Muhammad Riaz* and Saghir Ahmad****
GENETIC DIVERSITY IN UPLAND COTTON FOR COTTON LEAF CURL VIRUS DISEASE, EARLINESS AND FIBER QUALITY
...s cotton leaf curl virus disease (CLCuD). For estimation of genetic diversity regarding CLCuD tolerance, fiber quality and some yield contributing traits, 101 cotton genotypes imported from USA were evaluated. Different statistical procedures like cluster, principle components (PC) and correlation analysis were employed to identify the suitable genotypes that can be further exploited in breeding programme. Significant associations were found between yield cont...
Mazher Abbas*, Irfan Mehmood*, Arshed Bashir*, Muhammad Ather Mehmood* and Sonila Hassan*
WOMEN COTTON PICKERS PERCEPTIONS ABOUT HEALTH HAZARDS DUE TO PESTICIDE USE IN IRRIGATED PUNJAB
... importance (in terms of disease treatment and long-run health costs) of using safety measures during and after cotton picking....
Muhammad Mohsin Raza*, Muhammad Aslam Khan*, Irfan Ahmad**, Ali Ahsan Bajwa***, Hafiz Muhammad Usman Aslam*, Badar Ahsan Ullah****, and Kashif Riaz*
FOREST PATHOGENS AND DISEASES UNDER CHANGING CLIMATE -A REVIEW
...s and severity of forest diseases caused by diverse pests, resultantly altering the forest ecosystems. The present review covers the relationship between climate and diverse cases of forest diseases and potential shocks of climate change on pathogens and diseases. Biotic diseases, cankers, decays, declines, foliar disease<...
S. Talat Gilani*, Shahid Hameed* and Hussain Shah*
OCCURANCE AND DISTRIBUTION OF POTY VIRUSES INFECTING GARLIC IN PAKISTAN
...the poty viruses causing diseases in garlic (Allium sativum) from major garlic growing areas of Pakistan. The yellow stripes, mosaic and chlorotic spot symptoms of the disease resemble the viral infection in garlic reported to occur worldwide. Altogether 690 samples were collected from 29 locations of Punjab and 40 locations of Khyber Pukhtunkhwa to determine the prevalence of Onion Yellow Dwarf Virus (OYDV) and Leek Yellow ...
Basharat Hussain Shah*, F. S. Hamid*, Shams ul Islam*, Fayaz Ahmad*, Sohail Aslam*, and Noorullah Khan*
EVALUATION OF DIFFERENT PEA (PISUM SATIVUM L.) GENOTYPES FOR YIELD AND OTHER ATTRIBUTES AT SHINKIARI, MANSEHRA
...susceptible against this disease followed by 'Sarsabz 9800-1' as moderately susceptible and 9375 as resistant. The other varieties/lines Pea-09, Climax, Local and PF-400 were found tolerant against this disease. Green pod yield per hectare data revealed that pea variety 'Climax' produced the highest yield (11.6 t ha-1) followed by advance lines PF-400 (11.5 t ha-1) and 9375 (9.9 t ha-1), respectively. The lowest yield (2.1 t...
Ummad-ud-Din Umar*, Muhammad Aslam Khan**, *Ateeq-urRehman*, Abdul Hannan***, Syed Atif Hasan Naqvi*, Azhar Ali Khan****, Muhammad Asif Zulfiqar****
DEVELOPMENT AND VALIDATION OF POTATO LEAF ROLL VIRUS DISEASE PREDICTION MODEL BASED ON ENVIRONMENTAL FACTORS FOR FAISALABAD, PAKISTAN
...align: justify;"> A disease predictive model for Potato leaf roll virus (PLRV) was developed based on a ten year dataset of disease incidence and environmental variables and validated by two years data collected in Faisalabad, Pakistan. Maximum and minimum air temperatures, relative humidity and rainfall were employed as independent variables while PLRV disease incidence served as the...
Arshia Naeem*, Maria Anjum*, Mariam Rehman*, Zahid Mahmood** and Muhammad Asif Kamran**
AN INTEGRATED INFORMATION SYSTEM TO FACILITATE FARMERS IN WHEAT, SUGARCANE AND OTHER CROP DISEASES IDENTIFICATION
...entists to identify crop diseases. A case study was developed from Faisalabad region where information about crops, their soil conditions and associated diseases were provided on a web application. An online registration facility was provided along with interface to send image of crop related diseases. The desktop application consisted of an image analyzer that received images from web app...
Ghazala Nawaz*, Muhammad Nawaz Malik**, Muhammad Hassan
Mushtaq***, Fraz Munir Ahmad****, Ali Abdullah Shah*****, Farooq
Iqbal******, Shinawar Waseem Ali*******, Zahida Fatima******** and
Amjad Khan*********
SURVEILLANCE OF BRUCELLOSIS IN LIVESTOCK IN RURAL COMMUNITIES OF PUNJAB, PAKISTAN
...done in Pakistan. Active disease surveillance at door steps was conducted to collect blood samples from small and large ruminants of subtropical rural communities in the province of Punjab during January-March, 2015. Total 59665 sera samples from apparently healthy animals were collected and screened by Rose Bengal Plate test and confirmed by Complement fixation test, respectively. After adjustment for test sensitivities and specificities accordingly, and chi ...
Waqar Islam*
... threat by various plant diseases worldwide. A sudden epidemic breakout of any plant disease can cause huge economic losses leading towards the famine. To cope with this situation, understanding plant disease triangle and disease epidemic forecasting is very important. So here we have briefly introduced plant disease e...
Wazha Mugabe1,3,Gaolebale Segolame Mpapho1, John Kamau1, Wameotsile Mahabile2, Shalaulani James Nsoso1, Kealeboga Dipheko1 and Assar Ali Shah3,*
... farmers informed on the diseases only, 76% were able to diagnose the disease. Most diagnosis was based on clinical observations (68) with the low utility of California Mastitis Test (CMT) (3%). Milk production was at subsistence scale and a secondary product from meat type breeds. With a commercial mindset, improved goat management and cooperative pooling and marketing of milk the current farming setup of using meat breed f...
Anza Zahid*, Muhammad Ali Ayub, Mujtaba Saeed, Nasir Pasha, Abdul Jabbar Dar, Huzaifa Javed, Aimen Shakeel, Amna Nasir, Ana Akbar, Zainab Tarrar and Ayesha Humayun
...;">Diabetes is a chronic disease and non-compliance attitude of patients poses a great challenge to the success of therapy. Multiple factors influence compliance among diabetics and other chronic diseases and non-compliance results in avoidable consequences and complications. In order to determine the magnitude and factors of non-compliance to physicians’ advice in diabetics attending outpatients, a cross-sectional ana...
Naimat Ullah Khan1,2, Muhammad Hassan Saleem1, Aneela Zameer Durrani1, Nisar Ahmad3, Ayesha Hassan1, Muhammad Kashif Prince1, Muhammad Luqman Sohail4,*, Mian Saeed Sarwar2, Hazrat Ali2, Tahir Usman2, Asadullah Khan2, Siffat Ullah Khan3 and Shahid Zaman5
...tors associated with the disease (month, age, sex and season). Results showed highest prevalence (P < 0.05) of the Cryptosporidium in Kohat (33.33%) followed by Lakki Marwat (25%), while the lowest percent prevalence was recorded in Bannu (23.33%). Highest month-wise prevalence was recorded in the month of August (46.6%), followed by July (40%), and the lowest during February and December (16.66%). Results showed highest prevalence in summer season (...
Aitezaz Ahsan1*, Muhammad Usman1, Ihsan Ullah2, Aamer Bin Zahur3 and Adnan Rasheed Malik4
...R) is as a transboundary disease whose occurrence hampers the trade and consequently causes an enormous economic losses. It is a disease of sheep and goat, particularly in the regions of Africa, Middle East and Asia. Besides clinical signs and symptoms of PPR various diagnostic assays, both serological and molecular, have been developed and are applied for estimating the current status of the disease...
Abdul Nabi Jatt1,*, Sarfraz Ali Tunio1, Shaista Bano Memon1, Abdul Sattar Qureshi2 and Muhammad Aqeel Bhutto2
...rulence factors to cause diseases. The aim of the present study was to evaluate enzymatic activities of the bacterial strain Shigella dysenteriae IM using API-ZYM test system. Out of nineteen different enzymes, S. dysenteriae IM strain was capable to poroduce nine enzymes, i.e., alkaline-phosphatase, acidic-phosphatase, lipase, valine arylamidase, cystine arylamidase, napthol-AS-BI-phosphohydrolase, β-galactosidase, leucine arylamidas...
Aqsa Gulzar1, Tariq Mahmud1,*, Rubina Munir2 and Asma Anjum3
... on HeLa cells (cervical disease) and Luminol-improved chemiluminescence assay, respectively. The novel Schiff bases 4a and 4b have been synthesized by the reaction of 2-[(1,3-benzothiazol-2-yl)sulfanyl]-N-[4-(hydrazinecarbonyl)phenyl]acetamide with 3-nitro and 4-nitrobenzaldehyde. Characterization of the synthesized compounds was done by using various spectroscopic techniques namely FTIR, proton and carbon-13 NMR, Mass spectrometry and elemental analys...
Nasir Abbas1, Muhammad Suleman1, Nazir Ahmad Khan2,*, Ijaz Ali3, Mubashir Rauf1,5 and Sadeeq ur Rahman4,*
...s of chronic respiratory disease (CRD) in poultry and pheasantry birds of Northern Pakistan is underreported. In this study we report on the seroprevalence and isolation of MG from a total of 2341 poultry and 24 pheasantry birds. Overall, seropositivity of 46.56% (1090/2341) and 27.2% (6/24) against MG was observed among poultry- and pheasantry- birds, respectively. Broiler breeder and broiler showed 58.93% (264/448) and 37.23% (542/1456) seropositivity, respe...
Ahmad Farooq1, Usman Waheed2,3, Hasan Abbas Zaheer2,3, Abdul Rauf4, Abida Arshad5, and Muhammad Arshad1,*
...them to many blood borne diseases, most commonly hepatitis B and C. The aim of current study was to determine the prevalence of HBV and HCV infections among thalassaemia major patients. The study was conducted from June – December 2016, at the Thalassaemia Centre, Shaheed Zulfiqar Ali Bhutto Medical University, Islamabad and the Pakistan Thalassaemia Centre, Pakistan Baitul Mal, Islamabad, Pakistan. Data were obtained by clinical testing of 1,440 ß...
Naimat Ullah1,3,*, Aneela Zameer Durrani1, Muhammad Avais1, Nisar Ahmad2, Sana Ullah3, Muhammad Shuaib Khan3, Khalid Mehmood5, Mumtaz Ali Khan1 and Ikramul Haq1
...a common blood protozoan disease of sheep in tropical and subtropical areas. The current study was designed to inspect the prevalence and its correlation with various risk factors and host biomarkers concerned with the occurrence of theileriosis in sheep. Total 600 blood samples were taken each 200 from districts Bannu, Tank and Dera Ismail Khan of southern Khyber Pakhtunkhwa Pakistan and screened through blood microscopy. The current study revealed an overall...
Syeda Madiha, Zehra Batool, Saiqa Tabassum, Laraib Liaquat, Sadia Sadir, Tahira Perveen and Saida Haider*
...ferent neurodegenerative diseases.
...
Abdul Majid Ujjal2, Sharmin Akter1* and Suman Paul1
...al prevalence of buffalo diseases and to determine the temporal distribution of important infectious diseases of buffalo like as Foot and Mouth Disease (FMD) and Haemorrhagic Septicaemia (HS) at Upazila veterinary hospitals of Jaintapur, Gowainghat and Kanaighat upazilas of Sylhet district. From October, 2015 to September, 2017 a total of 1057 cases of buffalo dise...
Lotta Wahldén1, 3, Ulf Emanuelson1, Torsten Møller2 and Jonas Johansson Wensman1*
... fear of vaccine-induced disease. Kolmården Wildlife Park in Sweden has used a live attenuated vaccine without any apparent cases of vaccine-induced disease, but no studies to evaluate immune response after vaccination have been performed. The objective of this study was to gain more knowledge about immune response following vaccination of the African wild dog, in order to improve husbandry in zoos and help wildli...
Muzafar Shah1,*, Mazhar Ali2, Sardar Azhar Mehmood2, Shabir Ahmad2, Khushi Muhammad3, Israr Alam1, Kausar Saeed1
...or incidence of malarial disease. For this purpose a total of 997 individuals were screened for malarial test in the study area and divided into four category of age i.e. (1-15), (16-30), (31-45), (46-onward), gender wise, union council (Kota, Barikot, Shamozo and Ghalegi) and month wise. It was concluded that out of 997 samples, 204 (20.46%) were found positive, in which mostly under positive, under 15 year of age (23.36%), whereas less cases were reco...
Jehangir Khan1,*, Rasheedullah1, Bakht Tarin Khan2, Ayaz Ahmad3, Muhammad Salman4 and Khalid Khan5
...d with hepatitis and the disease is constantly increasing. Every 10th Pakistani is believed to be suffering from viral hepatitis. It is the first study documenting the epidemiology of HCV in District Shangla, Khyber Pakhtunkhwa (KPK). Blood samples from 900 individuals of various age groups were collected (August to November 2013) from patients at clinics, houses and pharmacies belonging to three Tehsils (Puran, Alpuri and Besham) of district Shangl...
Urooba Pervaiz1, Abdus Salam1, Dawood Jan2, Ayesha Khan1 and Mahmood Iqbal1
...ical knowledge, pest and diseases incidence, improper marketing of produce in the study area. ...
Jiji Li*, Tianyang Zu, Xiangli Dong, Xiao Yang, Wei Liu and Changwen Wu
...lp to develop methods of disease resistance in the aquaculture industry.
...
Ayesha Munir, Hafiz Muhammad Umer, Anjum Nasim Sabri and Imran Sajid*
...a significant infectious disease of human being caused by multispecies of bacteria. Among the causative agents of dental caries, notable one is Streptococcus mutans, which is an obligate parasite. In this study eleven S. mutans strains were isolated from carious lesions on Mitis Salivarius Bacitracin agar along with twenty potentially active Streptomyces strains were isolated from the soil of agriculture farmlands. The biochemical and phys...
Niamatullah Kakar, Farhat Abbas, Muhammad Shafee and Tauseef Asmat*
...) is airborne infectious disease and poses a great threat to public health. Precisely and timely detection of Mycobacterium tuberculosis is prerequisite for the successful treatment of TB. Aim of this study was to evaluate the prevalence of tuberculosis along with associated risk factors in Quetta Jail, Pakistan. In this cross-sectional study, 186 sputum samples were collected randomly from jail inmates. Sputum was processed for smear microscopy ...
Abdul Samad1, Ferhat Abbas1, Zafar Ahmad1, Olena Pokryrshko2 and Tauseef M Asmat1,*
... incidence of food-borne diseases. ...
Jun Chen1, Shuai Shang2, Xiaoyang Wu1, Jiakuo Yan1, Huaming Zhong1, Huanxin Zhang2, Weilai Sha1, Wanchao Zhu1 and Honghai Zhang1,*
...f great significance for disease monitoring and protection of these animals.
... 
increasing in growth parameters).
