Comparison of Two Set Pathotypic-Specific Primers to Detect Newcastle Disease Virus
Comparison of Two Set Pathotypic-Specific Primers to Detect Newcastle Disease Virus
Dwi Desmiyeni Putri1*, Nurhayati1, Intan Kamilia Habsari1, Ni Luh Putu Ika Mayasari2
ABSTRACT
The purpose of this research is to analyze the compatibility of two sets of pathotypic-specific primers to detect ND viruses circulating in Indonesia. This study used 4 ND isolates characterized by RT-PCR and amino acid sequencing (Putri et al. 2018). The 4 ND isolates used as isolates represented the NDV currently circulating in Indonesia. The study used 4 pathotype-specific primers. The first step of the study was to analyze the compatibility of the primers and the sequencing results of ND isolates using BioEdit® version 7.2 and MEGA version 11 sequence alignment editing software. The next step was to amplify the ND isolates using two sets of pathotype-specific primers. This amplification stage was carried out three times (as repetition). According to this study, it is known that virulent ND isolates can be amplified with pathotype-specific primers designed by Kant et al. (1997). However, the pathotype-specific primer (nested PCR) developed by Pham et al. (2005) could not amplify these isolates.
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