Identification and Characterization of a Novel Type of Cartilage Stem/ Progenitor Cells from Tibetan Mastiff
Identification and Characterization of a Novel Type of Cartilage Stem/ Progenitor Cells from Tibetan Mastiff
Caiyun Ma1, Hebao Wen1, Lu Li2, Gaofeng Liu1, Chunjing Wang1, Yu Guo1, Shiji Fang1, Hongyu Wang1* and Changqing Liu1*
ABSTRACT
Based on the physiologically non-self-renewing property of articular cartilage, the treatment of articular cartilage lesions remains an important challenge. Articular cartilage, the target of osteoarthritis (OA), contains renewable cartilage stem/progenitor cells (CSPCs) to maintain the tissue homeostasis. Compared with other adult stem cells, the self-renewal capacity and preference for chondrogenesis of resident CSPCs have propelled their exciting therapeutic prospects for cartilage repair. Tibetan mastiff, the ancestor of large breed dogs worldwide, represents a valuable model for human diseases. To our knowledge, this is the first study to capture the emergence of Tibetan mastiff-derived CSPCs. Our study aimed to unveil the biological properties and differentiation multipotency of CSPCs. CSPCs were characterized by the clonogenicity, growth curves and karyotype analysis, respectively. The results showed that the CSPCs of Tibetan mastiff displayed self-renewal ability, proliferative potential, and hereditary stability. Moreover, the CSPCs of Tibetan mastiff also positively expressed recognized surface antigens of human CSPCs. Additionally, CSPCs exhibited differentiation multipotency, including osteogenic, adipogenic and chondrogenic potential in vitro. These findings may propell CSPCs application in a clinical study, suggesting potential therapeutic treatment for OA or related joint diseases.
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