Efficacy of the sterile fungus GU23-3 on growth characters of cucumber plants and resistance against Papaya ring spot virus
Efficacy of the sterile fungus GU23-3 on growth characters of cucumber plants and resistance against Papaya ring spot virus
Shimaa A. Khalaf1 , Mohsen M. Elsharkawy2, Samir A. Sidaros2, Shawky A. El Kewey2, Ayman F. Omar2, Ali Hamed1
ABSTRACT
Background: Papaya ring spot virus (PRSV) is a major threat to the production of cucurbits all over the world. The sterile fungus GU23-3 is a rhizosphere fungus conferring positive effects on plant growth and health..
Objective: The aim of this study is to evaluate the effects of GU23-3 on cucumber growth and resistance against PRSV..
Methods: Disease severity and virus titer were evaluated in cucumber plants treated with barley grain (BGI) or culture filtrate (CF) of GU23-3 under greenhouse conditions. Additionally, the expression levels of defense genes were assessed by reverse transcription PCR (RT-PCR) analysis.
Results: Both of virus severity and titer were significantly reduced in treated plants in comparison with the control. All growth characters and yield of cucumber plants were increased in GU23-3 treated plants than the control. RT-PCR analysis revealed increased expressions of different pathogenesis related genes (Chitinase, β 1-3 Glucanase, PR1, POX, PAL and LOX) explaining the role of these genes in the mechanism of induced resistance against PRSV by GU23-3.
Conclusion: GU23-3 could be considered a new and alternative strategy to control PRSV infection.
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July 2019
Vol. 6, Pages 1-101
