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Variations in the Prion Protein Gene (PRNP) Sequences of Wild Punjab Urial from Pakistan

Variations in the Prion Protein Gene (PRNP) Sequences of Wild Punjab Urial from Pakistan

Nida Sadaqat1, Abdul Wajid2, Quratul Ain2, Muhammad Zia Akbar2Farkhanda Manzoor4, Muhammad Sarwar Khan1, Nasir Ali3,  Masroor Ellahi Babar3 and Tanveer Hussain3*

1Center of Agricultural Biochemistry and Biotechnology, University of Agriculture, Faisalabad
2Department of Biotechnology, Virtual University of Pakistan, 1-Davis Road, Lahore
3Department of Molecular Biology, Virtual University of Pakistan, 1-Davis Road, Lahore
4Department of Zoology, Lahore College for Women University, Lahore.
 
Nida Sadaqat and Abdul Wajid contributed as first author.

*      Corresponding author: tanveer.hussain@vu.edu.pk

ABSTRACT

Scrapie is a fatal neurodegenerative disease caused by the accumulation of improperly folded forms of host-encoded cellular-prion protein (PrPc) in the central nervous system (CNS) and in some peripheral tissues in sheep and goats. Scrapie resistant/susceptibility have been associated with the presence of PRNP gene polymorphisms. We analyzed the polymorphisms in PRNP gene sequences in 49 wild Punjab urial (Ovis vignei punjabiensis). Four novel amino acids polymorphisms (p.Q149E, p.Q155E, p.Y228L and p.L253F) were detected in PRNP gene. The urial was monomorphic at codon 149 and 155 and polymorphic at codon 228 and 253. These amino acid polymorphisms were combined in four alleles and six genotypes. Investigating the polymorphisms at codon 136, 154 and 171, the only genotype ARQ/ARQ was detected in Punjab urial. The PRNP gene sequences in Punjab urial showed low variability and is resemble to previous findings for wild animals. Prevalence-estimate, transmissibility, distribution, and host-range of prion disease in wild-animals remain undetermined.
 

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Pakistan Journal of Zoology

February

Vol. 54, Iss. 1, Pages 1-501

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