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Morphological and Immunophenotypical Properties of Chicken Bone Marrow-Derived Dendritic Cells Towards Wild Type and H5-Recombinant Fowlpox Virus Infections

Morphological and Immunophenotypical Properties of Chicken Bone Marrow-Derived Dendritic Cells Towards Wild Type and H5-Recombinant Fowlpox Virus Infections

Anis Suraya Mohamad Abir1, Sakinah Yusof1, Abd Rahaman Yasmin1,2, Abdul Rahman Omar1,3, Kok Lian Ho4, Wen Siang Tan1,5, Abdul Razak Mariatulqabtiah1,6* 

1Laboratory of Vaccine and Biomolecules, Institute of Bioscience, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia; 2Department of Veterinary Laboratory Diagnostics, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia; 3Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia; 4Department of Pathology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia; 5Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia; 6Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.


*Correspondence | Abdul Razak Mariatulqabtiah, Laboratory of Vaccine and Biomolecules, Institute of Bioscience, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia; Email: mariatulqabtiah@upm.edu.my 

ABSTRACT

Fowlpox is a common viral disease caused by fowlpox virus (FWPV), which infects birds. Fowlpox is an important disease in commercial poultry farming, as it can affect many production-system variables, such as size, performance and viability. Studies on the interactions between chicken bone marrow-derived dendritic cells (chBM-DCs) and FWPV and its recombinant viruses are limited compared to other avian viral infectious diseases. Therefore, this study characterised the response of chBM-DCs to infection with the wild-type (WT) FP9 strain of FWPV compared to a recombinant FWPV carrying the H5 gene of avian influenza virus (rFWPV-H5) using morphological and immunophenotypical observations. The results showed that the rFWPV-H5-infected chBM-DCs formed better dendrites and stellate cells, compared to the WT FWPV. The recombinant virus also expressed higher levels of the surface markers CD86 and MHC class II double-positive chBM-DCs 12 and 24 h post-infection. In conclusion, chBM-DCs were susceptible to WT FWPV and rFWPV-H5 infections, where the recombinant virus induced phenotypic maturation of the cells better than the WT virus. This study supports further understanding of the innate immune response after FWPV infection in chickens.

Keywords | Fowlpox, Avian influenza virus, Chicken, Dendritic cells, Haemagglutinin 

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Advances in Animal and Veterinary Sciences

June

Vol. 12, Iss. 6, pp. 994-1205

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