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John Shook

 

Science, Religion and Culture
... sciences and scientific methodologies to help explain why no gods are real. Folk religions and anthropomorphic gods can’t survive, but science vs. religion is hardly the whole story. Only science joined by philosophical reflection suffices to skeptically analyze the natural theology arguments for supernatural gods, too aloof and abstract for direct confrontation over evidence. Theology’s desperate maneuvers for avoiding science and scientific athe...

Timothy Helton

In a Mirror Dimly: Anthropology and Restoring a Sense of Presence to an Empty World
...ological conceptions and methods made by contemporary thinkers. Next, taking the work of E. B. Tylor as an example, I demonstrate the validity of such criticisms. Then, I offer two examples of alternatives to objectifying ethnography before tracing developments in anthropological method in the last decades that have greatly improved the lens used for the anthropological gaze. Finally, I conclude by suggesting the collaborati...

Farhad Zulfiqar, Muhammad Ashfaq

...cal zones in Punjab. The method of ordinary least square was used. Time series data for different explanatory variable (eco- nomic, location and climatic) from 1979-2009 relating to wheat were used for Faisalabad and Ba- hawalpur. The results of analysis showed that the effect of climatic variables was found significantly higher than that of non-climatic variables. The largest impact on wheat yield was of mean maximum average temperature at the time of maturit...

Raja Waqar Ahmed Azad, Tahir Sarwar

...rmined by inflow-outflow method, wherein the flow velocity was measured using current meter. The conveyance losses were found 13.2%, 11.8% and 7.8% in watercourses Sarai, Derkot and Mera Dadu, respectively, which resulted in conveyance efficiency of 86.8%, 88.1% and 92.4%, respectively. Results showed that conveyance losses increased and conveyance efficiency decreased with the aging of watercourse. Hence, more repair and maintenance is required for aged water...

Varadaraja V. Raman

...t (pre-determined and/or methodical) whose goal is to frighten and possibly kill people. Invading soldiers over the ages have terrorized innocent civilians in many regions of the world. Governments have terrorized their own people, the most glaring instance was in post-Revolutionary France during the Reign of Terror. On the other hand disgruntled, oppressed, and frustrated citizens (Irish Catholics, Algerian Muslims, Sri Lankan Tamils, etc.) have terrorized pe...

Chun-Yi Lin1, Meng-Ling Wu2, Tang-Long Shen1, Hsin-Hung Yeh3, Ting-Hsuan Hung1*

 

...plex molecular detection methods provide the understanding of the genetic diversities among viroid isolates and quantify viroids in citrus host. Our field survey can help clarify citrus-viroid relationships and develop proper prevention strategies in the near future.

 

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Faheem Khan*, Muhammad Zafarullah Khan

... A multi-stage sampling method was used to draw sample of 336 sugarcane growers for the present study and an interview schedule was used to solicit responses that addressed the research objectives. The findings from the study showed that 80 percent of respondents were illiterate while the 20 percent of respondents who were literate had achieved various levels of education. In the years 2012 and 2013, the average yield of educated sugarcane growers was 76 t/ha...

Thomas J. Coleman III1*, Ralph W. Hood Jr.1 and John R. Shook2

... the key theoretical and methodological issues surrounding them as well as their implications for the study of atheism and secularity.

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Ethan G. Quillen

...om a number of different methodological and theoretical approaches so as to develop a truly inter- and multi-disciplinary understanding about what we mean when we discuss Atheism. On the other, this myriad of voices has equally led to an ever-broadening discordancy, an equivocal discourse that makes it all the more difficult to identify any sort of common or universal definition. This latter issue is, as this paper will argue, the result of a theoretical abst...

Rick Repetti

...fferent frameworks, methods, and modes of discourse that are distinctively Buddhist, much of which it presents in its own modality.

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Mohammed A. Rohaim1*, Rania F. El-Naggar2, Abdulrahman M. Gamal3, Elshaimaa Ismael3, Mohamed M. Hamoud4, Sherif T. Moubarak3, Ashraf M. Metwally1, Manal M. Zaki3, Shimaa A.E. Nasr3, Samah Elsaid3, Mohamed M. Ali3, Hussein A. Hussein1 and Osama K. Zahran3

Email: mohammedvet1986@gmail.com; mohammed_abdelmohsen@cu.edu.eg

...act time. Numerical method and neutralization test were used to assess the ability of each disinfectant to inactivate the virus. Our results revealed that all the used disinfectants were effective with increasing the contact time more than 15 minutes except with Virkon®S which was effective even at a short contact time, 5 minutes. In conclusion, this study reported that chlorine and non-chlorine oxidizing agents are effective against H5...

Shumaila Manzoor1, Afshan Ahmed1 and Muhammad Abubakar2*

...y and specificity. These methods were performed by using set of sera collected from cattle with foot-and-mouth disease vaccination. The pattern of antibody titers in animals of different age groups was observed as less than 1 year, 1-2 years and more than 2 years for both ELISA and SNT. The peak immune response measured by SNT was 2.8log10 whereas ELISA detected serum antibody titer up to 2.4 log10. Findings showed tha...

Ummara Waheed Khan, Raza Ahmed, Irum Shahzadi and Mohmmad Maroof Shah

Email | mmshah@ciit.net.pk

...align: justify;">Various methods and techniques have been developed and tested to improve important cereal crop species since quite long time. Among the prominent techniques, traditional plant breeding has been on leading edge. The discovery of molecular genetics and biotechnological techniques, such as genome mapping, tissue culture, genetic transformation and in vitro regeneration offered new dimensions of research to improve important cr...

Mushtaq Ahmad1*, Habib Akbar1, Mohammad Tariq Jan1, Muhammad Jamal Khan Khattak2 and Abdul Bari3

...epth, nitrogen placement method and biochar on the growth, yield and its related parameters of sugar beet (Beta vulgaris L.) at Sugar Crops Research Institute Mardan during 2011-12 and 2012-13. The experiment was laid out in a randomized complete block design with split plot arrangement in four replications. Sugar beet was sown at the rate of 6 kg ha-1 at four seeding depths from 1 to 4 cm. Nitrogen was applied at band and broadcast me...

Shahid Ali1, Habib Akbar2, Mohammad Tariq Jan2, Mohammad Jamal Khan3 and Abdul Bari4

...ions and setts placement methods on the attributes of sugarcane were assessed at Sugar Crops Research Institute, Mardan-Pakistan. The experiment was conducted in RCB Design with split plot arrangement, over years (2012-13 and 2013-14). Planting sources i.e. standing (fresh canes frost exposed) trenched (stored canes frost protected) and canes portions top, middle, bottom and mixed 33 % each were assigned to main plots, while setts placement

Monireh Ahmadi Bani1*, Mostafa Kamali2, Iman Borji3, Soheyla Aslani3, Francis Fatoye4

...based on the old and new methods. Paired sample t-test was used for comparison between two methods and different conditions (eyes open and eyes closed). Traditional method for stability analysis showed no difference between two conditions in one minute standing (p ≥ 0.05). However, the new method confirmed that stability parameters significantly decre...

Ali Mohammadi, Mohammad Hossein Ebrahimi

...align: justify;">Various methods of treatment have been used for patients suffering from knee osteoarthritis (OA). Among them total knee artroplasty is being practised frequently. This method is suitable especially for elderly subjects with limited degree of physical activity. However, most of young subjects are not recommended to be treated with this method owing to high le...

Abiodun D. Olabode

...ield and farm management methods adopted in this area. Results were presented using charts and tables. The study revealed that; chemical fertilizers, manual weeding and shifting cultivation are essential for rice cultivation in the study area; the regression model established strong influence of farm-size at R-value of 0.64 on the yield of rice in this area; while estimated yields of 101.6tons and 57.9tons from irrigated and non-irrigated field respectively es...

Yongfeng Li, Mo Zhou, Xiao Wang, Libao Xie, Hua-Ji Qiu*

... as improving diagnostic methods and vaccines for classical swine fever.

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Anna-Maria Andersson*, Ann-Kristin Nyman, Per Wallgren

...ce interval based on the methodological variance for the VP2 ELISA. This confirms that the VP2 ELISA can be used for quantitatively estimating the ADV antibody levels even when the screening extends over a couple of weeks. For the long term consistency, a significantly higher mean ADV antibody level was recorded in February compared to in October. Therefore, if the VP2 ELISA should be used as a tool for selection of breeding females in a control program for AD...

Mohammed M. Islam1, 3 *, Damian F. J. Purcell2 and George Kannourakis1,3

...ed hosts. Several recent methodologies and chemistries have been proposed for viral diagnostics with variable outcomes and successes. In this review, we discuss a novel approach to identify the least transcripts of viral genomes using suppression subtractive hybridization that can be adapted to unanimously identify viruses of diverse genetic backgrounds. 

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Shahida Bibi* and Ijaz Ahmad Khan

...+ mungbean intercropping method for yield and yield attributes were studied under different crop combination at the Research Farm of The University of Agriculture Peshawar, Pakistan during the year 2012. The study was carried out in a split-plot design with three replications. Herbicide treatments (herbicide used and herbicide not used) were assigned to main plots, while intercropping treatments (sole maize, sole mungbean, 5 rows of mungbean + 6 rows of maize,...

Klara Fischer1*, Erika Chenais1,2, Emeli Torsson1, Jonas Johansson Wensman1

...ularly suitable research method to study epidemiology and social impacts of diseases in these contexts. However, for PE to achieve its full potential, stronger efforts to achieve true participation and to incorporate lessons about participation and power from the social sciences may be required. This review shows that social science engagement in PE to date is virtually non-existent, but that increased efforts to draw lessons from the social sciences and to in...

Amtul Jamil Sami1*, Madeeha Khalid1, Sara Iqbal1, Maira Afzal1 and A.R. Shakoori2

...cient and cost effective method to treat waste water polluted with carcinogenic dyes by coagulation-flocculation. Moreover this method is beneficial over other methods that employ toxic polymers or mineral coagulants.

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Robina Karim and Dawood Jan

...d purpose, study adopted methodology which required testing of hypothesis which states “Pakistan’s foreign trade is expected to be largely better off once WTO’s regime is fully implemented in Pakistan and rest of the world”. The statistical results show that, the coefficient of variation of Pakistan exports trade estimates at 0.1657 during 1985-90 and 0.0936 during 1990-95 pre-WTO periods, which with the exception of sub-period 2000-05 ...

Atul Sharma, Aruna Chandra Singh, Gautam Bacher, Sunil Bhand

...ion, reported analytical methods, advancement in biosensing platform especially regarding electrochemical transduction are discussed in detail. Finally, future prospects toward the development of selective and sensitive aptasensors for antibiotic detection are presented.

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 Ali Murad Rahoo1,2, Tariq Mukhtar3,*, Simon R. Gowen1, Rehana Kanwal Rahoo4 and Shaukat Ibrahim Abro5

...ence of IJ by using both methods was non-significant. Similarly, with an increase in inoculum dose and time, invasion of host was significantly increased. There was little invasion at the 800 IJ dose even after 4 days whereas with the commercial dose (8,000), nematodes did migrate to the larvae and caused some infection after 2, 3 and 4 days. Greatest invasion took place with the highest dose (80,000) with 8 IJ successfully finding and penetrating the larvae. ...

Taghreed A. Hafiz1, Murad A. Mubaraki1*, Mohamed A. Dkhil2,3 and Saleh Al-Quraishy2

...s prepared by maceration method and the total polyphenolic and flavonoids contents were determined using a spectrophotometric method. The antiradical scavenging property and the maximum nontoxic concentration of the extract was determined using DPPH assay and MTT method, respectively. Serial concentrations of the extract not exceed the maximum nontoxic concentration were used against ...

Süleyman Kök1*, Sertaç Atalay1, Hasan Semih Eken2 and Mustafa Savaşçi3

...zyme, was cut using RFLP method. The allele variants (G/C) of CAPN1 316 and (C/T) of CAPN1 4751 were determined with ARMS-PCR method. This study was conducted to determine three SNPs which were related to meat quality in 130 purebred female and male samples of TGC breed. In TGC samples, C allele frequencies related to UoG-CAST, CAPN1 316 and CAPN1 4751 polymorphisms were found to be 0.6038, 0.1115 and 0.5654, respectively. C...

Abdur Rahim1, Ghulam Abbas1, Muhammad Naeem2, Sara Ferrando3, Lorenzo Gallus3, Noor Khan4, Muhammad Hafeez-ur-Rehman4, Abdul Ghaffar5 and Abdul Mateen6

...ssing units, traditional methods were applied causing quality problem. Finally, it was concluded that quality of fish meal and production in our region is suitable for fish feed formulation. But the processing technique should be improved to match international standard, and to avoid declining in production of fish meal strategic management of fisheries resources will be required.

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Rongrong Li1, Kun Li2, Xiaoqiang Wang2, Houqiang Luo2, Gang Qiu2, Hui zhang2, Yanfang Lan2 and Jiakui Li2,3*

...27.8% in females. By the method of IHA, the seroprevalence in Tibetan pigs were 21.6%, and the prevalence was found 23.1% and 21.4% in males and females, respectively. The results indicates that the Tibetan gigs were exposed to T. gondii in Tibet area, which should arise a public concern of the threat to human and other animals in this unique region of the world.

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Sezgi Arman* and Sema Isisag Ucuncu

... with different staining methods of alcian blue (AB) at pH 2.5, aldehyde fuchsin (AF), periodic acid-Schiff (PAS), KOH/PAS and PAS/AB (pH 2.5) and investigated. Goblet cells of anterior intestine were colored strongly by AB (pH 2.5), AF and PAS; and moderately by PAS/AB (pH 2.5) (AB dominant). In the mid part of the gastrointestinal tract, goblet cells were also stained strongly with AB, PAS and PAS/AB (pH 2.5) (AB dominant), however, their reactions to KOH/PA...

Sezgi Arman* and Sema Isisag Ucuncu

... with different staining methods of alcian blue (AB) at pH 2.5, aldehyde fuchsin (AF), periodic acid-Schiff (PAS), KOH/PAS and PAS/AB (pH 2.5) and investigated. Goblet cells of anterior intestine were colored strongly by AB (pH 2.5), AF and PAS; and moderately by PAS/AB (pH 2.5) (AB dominant). In the mid part of the gastrointestinal tract, goblet cells were also stained strongly with AB, PAS and PAS/AB (pH 2.5) (AB dominant), however, their reactions to KOH/PA...

Neelum Andaleeb and Munir Khan

...a. A multistage sampling method was used to select a sample of 274 livestock farm households from 6 union councils, and a well-structured questionnaire was used to collect data from the female respondents through face to face interview. Cobb-Douglas production function and cost benefit analysis were used to arrive at the main conclusion. Results show that feed cost was the major component of total cost followed by labor cost. Average milk produced by buffalo a...

Sana Irshad Khan1*, Zafar Iqbal2, Hamid Ullah Shah2 and Shaukat Hussain3

...Ac) using disc diffusion method. However, the same concentration of crude (1 µg.mL-1) showed 62.20 ± 2.51% growth inhibitions against Fusarium oxysporum using agar well diffusion method. Further investigation, including various chromatographic and spectroscopic analyses on the separation and isolation of potent metabolites is suggested to get a useful lead structure for development of novel antibiotics to combat...
Yusuf Koc1, Ecevit Eyduran1* and Omer Akbulut2
...and CART regression tree methods for different combinations of parent node: child node in the data set regarding animal science. To achieve the aim, 1884 Mengali lambs were provided for predicting weaning weight from sex (male and female), birth type (single and twin), birth year (2005, 2006, 2007, 2008 and 2009), farm (Research station, Mastung, Quetta, and Noshki), birth weight, dam age, and dam weight. To choice the best regression tree
Eman Mahmoud El-Diasty1,Madeha Abd El-Halim Ibrahimand Ghada Kamal El Khalafawy2,*
...apings). Phenotype-based methods for identifying yeast especially Candida species are often difficult, time-consuming and not enough to identify most yeast species. . Molecular biological techniques provide a useful alternative approach. Concerning with genotypic identification, polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was applied for identification of Candida species, Cryptococcus albidus, Saccharomyces, Trichosporn, Torul...
Irshad Ahmad
...l and biological control methods for the substantial control of walnut borer. The present study offers valuable information through IPM, which appears to be a promising approach for the control of walnut borer. Collectively this strategy has resulted in significant reduction of the pest population in the target area of district Dir, Pakistan.
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Amjad Islam Aqib1, Muhammad Ijaz1,*, Aneela Zameer Durrani1, Aftab Ahmad Anjum2, Riaz Hussain3, Saba Sana2, Shahid Hussain Farooqi1, Kashif Hussain1 and Syed Saleem Ahmad1
...iotics by disc diffusion method. Over all mastitis was found 52.5% (63/120) with leading subclinical (41.67%, 50/120) form of mastitis on overall milk collected data. Pathogen type was crowned by Staph. aureus with 74.5% (47/63) prevalence, following which were Streptococcus species (17.45%, 11/63), E. coli (3.17%, 2/63), and Bacillus cereus (4.76%, 3/63). Coagulase positive Staph. aureus (41 out of 47), and hemolysin produci...
Faiz Muhammad,1, 2,*, Zhang Zhi-Feng,1, Shao Ming-Yu1 and Muhammad Shafi3
... (RACE) and anchored PCR method. The full length of the (LvCypA) had 855 bp, containing 495 bp of open reading frame (ORF), encoding 164 amino acids with an estimated molecular mass of 17.6 kDa. The LvCypA has four β- strands. Tissue distribution of the LvCypA after real time analysis revealed that expression is in order of muscle, gill, lymphoid organ and hepatopancrease. 
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Sadaf Ijaz Malik1, Kiran Afshan2* and Mazhar Qayyum1
... traditional microscopic methods on the basis of standardized measurements. Amphistomes were flattened and stained in Borax carmine, some were sectioned in the median sagittal plane and histological slides of the worms were prepared for detailed morphological studies. Bile tissues, aspirates and blood samples of the same animals were analysed. The infection was found prevalent throughout the year but during post monsoon season it was relatively high. Morpholog...

 Nosheen Noor Elahi* and Misbah Mughal

...hultz’s maceration method followed by staining with chloloiodide of Zinc. The results indicated substantial differences among the sugarcane varieties for various stem epidermal cell combinations and structures like number of stomata, number and shape of cork, silica, long, and pointed elongated cork cells. It is therefore contended that the structure of the epidermis in sugar cane can be used as a characteristic of variety differentiation.

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Akbar Ali1,*, Khalil Mohamed2 and Fawzia Toulah3

 

...tected in serum by ELISA method. Toxoplasma gondii DNA was detected using PCR. Most women were in the 20-29 years age group (48%), followed by 30-39 years (33%) and >40 years (14%). Overall prevalence of Toxoplasma gondii IgG was seen in 12%, with significantly more in women with age more than 30 years as compared to those with age less than 30 years (19% vs 8%; p=0.038). The percentage of IgG seroprevalence was higher in women who ever...
Mirza Imran Shahzad1,*, Anna Iqbal2, Farrah Ali1, Nuzhat Sial3, Muhammad Ashfaq4, Abul Hasanat5 and Azra Khanum6
... by using disc diffusion method. Supernatant fraction of E. coli was positive against Sal. typhi and supernatant fraction of P. multocida was positive against E. coli, which show that antimicrobial agents were secretory in nature. Sonicated fractions were on second order to produce antimicrobial activities. The freeze thaw method was least effective in secreting active antimic...

Neelum Andaleeb, Munir Khan and Syed Attaullah Shah

...n. A multistage sampling method was used to select a sample of 274 livestock farm households from 6 rural Union Councils, and a well-structured questionnaire was used to collect data from female respondents through face to face interviews. A composite index was constructed to measure the extent of women’s participation, and a linear regression model was used to determine factors affecting women’s involvement in livestock farming. Results reveal tha...
Abdus-Subhan1, Qudratullah Khan1, Muhammad Mansoor2, Muhammad Jamil Khan1, Ammanullah2, Matiullah Khan3
...erization (P) and sowing methods (S) on Water Use Efficiency (WUE), wheat yield and economics, a field experiment was carried out at Arid Zone Research Center, Dera Ismail Khan, Khyber Pakhtunkhwah Province, Pakistan, during rabi season of 2014-15 and 2015-16. The experiment was laid in randomized complete block (RCB) design with split plot arrangement keeping pulverization viz. single tiller P1, double tiller P2, triple tiller P3

Nirbhay Kushwaha, Achuit K Singh, Brotati Chattopadhyay and Supriya Chakraborty

Recent advances in geminivirus detection and future perspectives
...ry ago.Agreat variety of methods have been developed that permit differentiation of viral pathogens.These methods, initially based solely on identifying the distinct biological characteristics of different viruses, were soon supplemented with methods based on light or electron microscopy and serology and subsequently by Enzyme Linked Immunosorbant Assay (ELISA) and finally the use of molec...

B. Ramanujam, R. D. Prasad, S. Sriram and R. Rangeswaran

Mass production, formulation, quality control and delivery of Trichoderma for plant disease management
...ops. There are two major methods of inoculum production of Trichoderma spp. viz., solid state fermentation and liquid state fermentation. In solid fermentation, the fungus is grown on various cereal grains, agricultural wastes and byproducts. The solid state production is highly labour intensive and fit for cottage industry. These products are used mainly for direct soil application in nurseries/main fields to suppress the soil-borne inoculum. In liquid state ...

M. S. A. Mamun and M. Ahmed

Integrated pest management in tea: prospects and future strategies in Bangladesh
...paration and application methods of new botanicals and microbial pesticide formulations, evaluation of field bio-efficacy and conservation of biological agents, cultural control measures, genetic techniques and sex pheromones technology. In tea husbandry, cultural control measures such as plucking, pruning, shade regulation, field sanitation, fertilizer application, manipulation or destruction of alternate hosts and selection of pest resistant/tolerant varieti...

Dipak Mandal, Paramita Bhowmik, Pronobesh Halder and M. L. Chatterjee

Determination of relative toxicity and base line data of dif-ferent insecticides against cotton mealybug (Phenacoccus solenopsis Tinsley)
...ated food treated insect methods were followed. The lowest LC50 values through treated food untreated insect method were recorded in chlorpyriphos 20 EC (226 ppm) and dichlorvos 75 EC (282 ppm) in treated food treated insect method, which was 8.89 and 7.13 times more relatively toxic in respective method after 24 hours when compared to the standard check...

Anil Sehajpal, Saroj Arora and Parminder Kaur 

 

Evaluation of plant extracts against Rhizoctonia solani causing sheath blight of rice
...luated by disc diffusion method. Out of 44 plants tested, 36 plant extracts showed varied degree of antimicrobial effect at different concentrations against the pathogen whereas 8 plant extracts, viz. Abrus precatorious, Acacia auriculiformis, Bougainvillea glabra, Convolvulus arvensis, Hibiscus rosa-sinensis, Morus alba, Thevatia peruviana, and Withania somnifera did not exert any effect. Among all the plant extracts, A. sativum exhibited strong fungitoxicity...

Dhananjoy Mandal 2K. Baral 3M. K. Dasgupta

Developing site-specific appropriate precision agriculture
... by us in this trial for methodology development. As a prelude to SSAPA we are comparing nine rice- and vegetable-based cropping sequences between improved and farmers’ practices in terms of five parameters, viz. crop growth and productivity, soil nutrient management, pest management, (BCA) biocontrol agent balance, and energy balance, economics ~ each with several variables. We are also providing ground and prepare to provide PA –specific data to ...

Partha Pratim Ghosh and 2N. C. Mandal 

Some disease management practices for bacterial wilt of potato

T. P. Ghosh, D. Mandal, S. Laha and M. K. Dasgupta 

Dynamics and severity model in managing fungal diseases
...t to be predicted, a new methodology has been developed for indirect assessment in terms of severity as a direct function of yield loss in terms of occurrence or intensity but not yield or yield loss per se. These findings may help in building simple decision rules for management early in the season as soon as the disease appears in one case, and when some intensity has been achieved in all other cases. Where validated this approach may be a useful tool in pla...
Bao-Ming Ge* Dai-Zhen Zhang, Qiu-Ning Liu, Sen-Hao Jiang, Jun Cui, Chun-Lin Zhou and Bo-Ping Tang
...;s patchiness regression methods in each season (P< 0.001). The abundances were markedly affected by habitat type (F5,120 = 49.409, P < 0.001) and season (F3,120 = 13.577, P < 0.001), however, there was no significant interaction of habitat type and season (F15,120 = 0.529, P = 0.919). The abundances distribution was significantly different among habitats in each season as shown by one-way ANOVA, the higher abundance c...
Bushra Niaz1,*, Tahir Zahoor1, Muhammad Atif Randhawa1 and Amer Jamil2
...DS-PAGE). Disc diffusion method was applied for the evaluation of antibacterial activity of lactoferrin. Minimum inhibitory concentration (MIC) of lactoferrin was determined by 96-well plate method using ELISA reader. Results depicted that CM-Sephadex C-25 was a good cation exchanger for recovery of lactoferrin. Camel raw milk produced 2.4 mg/mL of lactoferrin. The lactoferrin isolated from camel milk exhibited promising ant...
Sohail Ahmed, Muhammad Arshad and Babar Hassan*
...n in contrast to coating method. Drying of woods prior to resin application was also effective in resisting termites’ infestation. The findings of treatment with resins are discussed with previously cited effects of resins and its implication in wood preservation.
...
Fariha Imtiaz1, Muhammad Islam1, Hamid Saeed2,*, Bushra Saleem1, Maryam Asghar1 and Zikria Saleem2
... hot and cold extraction methods. Phytochemical analysis of leaves was performed on powder and extracts of leaves using already reported methods. Mineral analysis was done using atomic absorption spectrophotometer. Hair growth promoting effects were examined using alopecia mouse model. Phytochemical analysis demonstrated the presence of higherquantities of carbohydrates, proteins and secondary metabolites in methanol compare...

Shahid Iqbal Khattak1*, Mohammad Safdar Baloch1, Khalid Naveed2 and Ejaz Ahmad Khan1

...compared to soil applied method. However, maximum grain protein content was recorded in soil applied method in comparison to foliar N application. In conclusion, higher results for all the quantitative parameters were observed at 120 kg N ha-1.

...

Shah Saud Ahmad and Muhammad Zafarullah Khan*

...hnology (94%) and sowing methods (93%) learned through FSC. Mass contact method was rarely used in the study area as only 10% of the respondents ranked it very high and 28% respondents reported it very low on likert scale. It can be concluded that FSC had developed the linkages of the respondents with agriculture research and water management department in the study area. Top learned skills from this approach were fertilizer...
Wu Yi-qun* and Xu Xiu
...h instantaneous-scanning method in the intersection area of the Yellow River, the Wei River and the Luo River located at the middle reaches of Yellow River basin of China. The results showed that standing accounts for 32.75 % of wintering behaviors for Great Bustard, followed by the feeding behavior (27.55 %) and walking behavior (16.69 %). Alert and lying behavior account for a small proportion – 8.45 % and 7.49 %, respectively; the rest behaviors take ...
Muhammad Imran Shabbir
...ribed here is a line-dot method using ternary genotype codes to determine genotype probabilities in a very quick and simple way. Also ternary genotype codes along with gender symbols and affection status are used to modify the Punnett squares. On applying these modified Punnett squares along polygonal shapes covers all possible parental combinations and their genotypic and phenotypic outcomes in offspring. In this way, simple illustrations incorporate a consid...
Koksal Karadas1* and Ibrahim Hakki Kadırhanogullari2
...lected with census study method in Igdir province of Turkey with the purpose of determining some factors influencing average honey yield (AHY) per beehive in the year 2014. For this purpose, predictive performances of several data mining algorithms (CART, CHAID, Exhaustive CHAID and MARS) and artificial neural network algorithm (Multilayer Perceptron, MLP) were evaluated comparatively. Several factors thought as independent variables in the survey were age of ...
Ghulam Abbas1,Asif Nadeem1,*, Masroor Ellahi Babar2, Tanveer Hussain2, Muhammad Sajid Tahir3, Wasim Shehzad1, Rajput Zahid Iqbal3, Muhammad Tayyab1 and Maryam Javed1
...ral resources. Molecular methods have an important role in phylogeny and diversity analysis. In Pakistan hog deer (Axis porcinus) is an important wild species belongs to the family Cervidae. The hog deer is one of the least studied species at molecular level in Pakistan. The present study was planned to investigate its molecular phylogeny and diversity analysisusing mitochondrial cytochrome b, cytochrome c and d-loop region. Samples...

Sania Shaheen1*, Hina Fatima2 and Muhammad Azeem Khan3

...stify;">In Pakistan, two methods mainly using for the transplanting of rice paddle (Conventional method) and Direct seeded method (Dry method) in rice growing areas of Punjab. In last some years, the direct seeded rice system is introduced in some of the rice cropping districts of Pakistan. The current research estimate the technical efficiency of conven...
Muhammad Aslam Farooqi1,*, Mansoor-ul-Hasan2, Sohail Akhtar1Muhammad Arshad2, Muhammad Naveed Aslam3 and Muhammad Rafay4
...mple and fast analytical method using High Performance Liquid Chromatography with ultraviolet detection (HPLC-UV) was used to determine residues of commonly used agricultural insecticides (imidacloprid, thiametoxam, profenofos, endosulfan, spinosad and deltamethrin) in multi-floral raw honey of Apis dorsata F. collected from the cotton belt area of Punjab, Pakistan. The residues of these insecticides were extracted using ethyl acetate. Honey samples wer...
Fareeda Tasneem1, Farah Rauf Shakoori1 and Abdul Rauf Shakoori2,*

 

...ed by maximum likelihood method placed the FT8 strain very distinctly from other species, giving it a clearly separate position. Phylogeny results based on the H4 gene sequences corresponded to the results obtained by 18SrDNA sequences. Both markers proved the FT8 strain is a highly divergent species based on the phylogenetic relationships, opening new realms for the researchers of this field.
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Sumaira Maqsood1,*, Muhammad Afzal1, Muhammad Anjum Aqueel1, Abu Bakar Muhammad Raza1,Waqas Wakil2 andMuhammad Hussnain Babar3

 

...using diet incorporation method. Larvae were allowed to feed for 48 h. Mortality was recorded until larvae died or pupated. Second instar larvae showed more susceptibility as compared with 4th instar larvae. Maximum mortality was recorded in 2nd instar larvae (91.02±2.04%) in comparison to fourth instar larvae (69.35±1.66%) in combined application of NPV and flubendiamide. Dose of flubendamide was the key factor for type of ...

Raza Ullah1*, Qaisar Shah Safi2, Ghaffar Ali3 and Irfan Ullah

...actices, proper time and methods of harvesting, packaging, storage and processing. There are various middlemen (market intermediaries) involved at different stages in the marketing of citrus. This study is an attempt to examine the marketing margins of the intermediaries involve in the value chain of sweet oranges of citrus group. Three villages in Bunir district are randomly selected and a total of 120 sampled respondents are selected using proportional alloc...
Kadir Karakuş1, Bahat Comba2, Abuzer Taş3, Tunahan Sancak3, Arzu Comba2, Devrim Saripinar Aksu4, Hasan Koyun5 and Mohammad Masood Tariq6,*
... of different castration methods in 4-month-old male Norduz sheep resulted in changes in the blood hormone levels, it was determined that these changes did not affect body weight. In conclusion, the evaluation of the process in castrated six-month-old sheep revealed an increase in the body weight which was similar to that in the control group.
...
Omar S.O. Amer1,3,*, Mohamed I. Waly2,4 andSaeed A. Al-Zahrani1
...ng different statistical methods to develop effective prevention and control strategies.
...
Lu Liu1, Sher Khan Panhwar2, Tianxiang Gao3, Zhiqiang Han3, Chunhou Li4, Dianrong Sun4 and Na Song1,*
...the species by molecular methods, we sequenced a fragment of the cytochrome oxidase subunit I (COI) gene of mitochondrial DNA of Liza affinis and Liza klunzingeri from coastal waters of China and Pakistan, respectively. Sequences of L. carinata were obtained from GenBank. A neighbor-joining tree showed the specimens to form a strong monophyletic group. The mean within-species genetic distances for L. affinis, L. carinata, and...

Jawad Anwar* and Zafar Iqbal 

...ed, using disc diffusion method against tomato wilt causing bacteria (Xanthomonas campestris and Clavibacter michiganensis). Extracts that resulted in strong activity were fractionated into acetonitrile and ethyl acetate followed by testing against human pathogenic bacterial strains (Eschericia coli, Pseudomonas aeruginosa and Staphylococcus aureus) using 96 well microtitre plate method. The Ethyl acetate fraction showed max...
Mohammad Umar Farooq1*, Sarfaraz Ahmad2, Ghulam Nabi1, Ijaz Ali1 and Imtiaz Ahmad1
...stion and Dry Combustion method.Four transect lines were drawn in each experimental plot. Twenty four samples of each experimental plot were collected with the help of ADC one m 2 quadrate methods, weighed and then oven dried at 60 oC to find out the dry weight for above pytomass carbon Mg C ha-1 (Brown and Lugo, 1982). Similarly, the same 1 m2 quadrate area was dug up to the depth...
Wanchao Zhu1, Qinguo Wei1, Shuyu Xue1, Huanxin Zhang2, Tianshu Lv1 and Honghai Zhang1,
...atellite markers using a method of fast isolation by AFLP of sequences containing repeats (FIASCO) from them in this study. The primers designed by Primer Premier 5.0 based on conservative regions were tested on 22 individuals. Our results demonstrated that the number of alleles per locus ranged from two to five and the mean number of alleles was 3.67. The observed and expected heterozygosities ranged from 0.227 to 0.545 and 0.201 to 0.630, respectively. The p...
Zuhao Huang*, Meifang Li, Rujue Ruan and Jiawei Qin
...ized. Maximum likelihood method was used to reconstruct the phylogenetic trees based on HKY+ G model. The phylogenetic tree grouped members of every genus were grouped into each clade, except the genus Gorsachius. Our results indicated that the genus Gorsachius might not be monophyletic.
...
Muhammad Usman Qamar1, 2, Sidrah Saleem1, Usman Arshad1, Muhammad Farhan Rasheed3, Hasan Ejaz4, Naveed Shahzad5 and Shah Jahan6,*
...st and disc potentiation method, respectively. The blaNDM-1genewas amplified by PCR and sequenced. MIC of different antibiotics were determined by VITEK 2 system and antibacterial activity, MIC and MBC of manuka honey was performed by agar well diffusion assay and microbroth dilution assay respectively. All isolates (n=5) were carbapenamase and MBL producers andNDM-1 gene carrier. These isolates displayed significant resistance against common...
Shakeel Ahmad Anjum, Muhammad Farrukh Saleem, Muhammad Shahid, Abdul Shakoor*, Muhammad Safeer, Imran Khan, Ayesha Farooq, Iftkhar Ali and Usman Nazir
...e objectives to optimize methods of B and Zn application in maize and ultimately enhance profitability. The experiment was laid out in randomized complete block design with factorial arrangement during autumn 2015. Treatments were replicated three times. The treatments were comprised of C = no Zn and B (Control); Zn (S) = soil application of Zn @ of 12 kg ha-1; B (S): soil application of B @ 3 kg ha-1; Zn (F) = foliar application of 1% Zn...
Faiz Muhammad1,*, Syed Khurram Fareed1, Urooj Zafar1, Taseer Ahmed Khan2 and Aqeel Ahmad1
...nt DNA crude preparation methods were used and broth dilution method found simpler and most efficient for positive PCR. Primers were selected for 16s rRNA and it could detect up to 100 cfu and 250 cfu from MS and MG respectively in a samples. We have tested pure DNA of other mycoplasmas (5 species from avian origin and 3 other mycoplasmas species) but it produced only the genus specific band. The optimized ratio of tPCR prim...
Saba Manzoor1,*, Ali Raza Awan1, Abdul Wajid1, Sehrish Firyal1, Muhammad Tayyab1, Muhammad Mansha2, Asim Khalid Mahmood3, Abu Saeed Hashmi1 and Muhammad Wasim1,*
... using Sanger sequencing method and RT-qPCR for the mutation and expression profiling of c-Myc gene. The coding region of c-Myc gene (exon2 and 3) did not show any variation in both species. While expression of c-Myc gene was found to be up regulated in different canine (62.5% in mammary adenocarcinoma, 75% in oral squamous cell carcinoma, 66.6% in peri-anal sac adenocarcinoma, 80% in mast cell tumor and 100% in soft tissue sarcoma) and fe...

Sikandar Hayat, Khalid Usman* and Muhammad Tahir Amin 

...N fertilizer application method may be a possible solution. Field trials were carried out to explore suitable row spacing and N fertilizer application method for zero tillage wheat at Agronomic Research Area, Faculty of Agriculture, Gomal University, Dera Ismail Khan (D. I. Khan), Pakistan in 2015-2016 wheat growing season. The experimental design was randomized complete block with split plot arrangement replicated thrice. F...
Mubashir Hussain1,Safdar Ali1, Muhammad Naveed Tahir1, Ghulam Abbas Shah1, Ijaz Ahmad2*, Muhammad Aqeel Sarwar3 and Sohail Latif4
...e compared viz., sieving method and seedling emergence method. The data were collected on the seed density m-2, seed frequency, diversity of weed species and relative importance value. The input and output data was also collected to find out the socioeconomic feasibility of the techniques. The comparative analysis of seed bank extraction methods revealed higher weed seeds densit...
Tahira*, Muhammad Arshad, Muhammad Ayub Khan and Mubashar Ahmad Khan
...is based on WARD’s method showed considerable genetic variation among the hybrids. In the first year, thirty- six Brassica hybrids were grouped into thirteen clusters. Cluster-IV comprised of maximum number of hybrids (six). The clusters mean and standard deviation marked that hybrid in Cluster XI (CRH-80) was short durational and high yielding mainly due to more branches and pods per plant.CRH-35 in cluster-VI was high yielding and has more seeds per po...
Muhammad Qaisar Nawaz
...d conditions. Two sowing methods i.e. broad cast and drill sowing with 30 cm apart rows and four nitrogen levels (75,100,125 and 150 % of N recommended dose i.e. 150kg ha-) were tested. Recommended dose of PK fertilizer (85-60 PK kg ha-1) was used uniformly with experimental N rates. Data on plant height (132.00 cm), number of plants (91.33 m-2), number of tillers (146.00 m-2), number of leaves tillers-1 (...
Semra Benzer1,* and Recep Benzer2
...mation result of the two methods which are Length - Weight Relations and Artificial Neural Networks from Uluabat Lake regarding narrow-clawed crayfish (Astacus leptodactylus Eschscholtz 1823) between 2015 and 2016. The relationships between total length (TL) and total weight (TW); carapace length (CL) for Astacus leptodactylus caught from Uluabat Lake were presented with tradional method of length-weight relati...

Imran Ali Rajput1*, Tajwer Sultana Syed1, Ghulam Hussain Abro1, Imran Khatri1 and Abdul Mubeen Lodhi2 

...were used in traditional method. The overall results showed that the sprays at different interval indicated the highest pest population reduction at tobacco (17.45-15.09%) followed by neem (14.58-15.33%) and datura (11.72-7.81%) in both varieties and similar trend was also noted in the second year of the study. The effect of varietal difference of Bt. and non-Bt. cotton varieties was not much recorded in the 2015 but later in 2016 the dual effect of tobacco an...
Temel Gokturk1, Elif Tozlu2,* and Recep Kotan2
...ntal issues, alternative methods were sought in the past; and in this context, studies were conducted to find new methods in which fungi and bacteria were used in the biological control against pests. Totally 10 bacterial strains including 2 strains of Brevibacillus brevis (CP-1, FD-1), 1 strain of Bacillus thuringiensis (FDP-1), 2 strains of Bacillus thuringiensis subsp. kenyae (FDP-8, FDP-42), 2...
Falah Baiee1,3, Wahid Haron1,*, Rosnina Yusoff1, Ariff Omar,2 Nurhusien Yimer1, Salman Hammadi1, Tarig Ahmedeltayeb1, Asmatullah Kaka4
...n by electro ejaculation method. The normal electro ejaculation method of semen collection (Method I) was compared to a modified method involving three stages of graduated electrical stimulation (Method II) in four crossbred bulls. The results showed that intensive muscle spasm, bull struggling and arc back were reduce...
Shumaila Ashfaq1, Liaqat Ali2*, Muhammad Ashraf Zia2, Rizwan Ahmad Khan2 and Mehtash Butt3
...veral factor and various methods have been used to reduce and treat the pain of PDPH. We evaluated effects of hydrocortisone on the treatment of PDPH in obstetrical patient.To compare mean decrease in Visual Analogue Scalepain score in patients who developed PDPH after elective caesarean section under spinal anaesthesia who were given conventional treatment versus conventional treatment plus hydrocortisone.Double blind randomized control trial. Study conducted...
Wali Khan1,*, Noor-Un-Nisa2 and Aly Khan3
... smear and concentration methods. One hundred and fifteen (11.04%) participants were found infected with one or more than one intestinal protozoans. Forty one (35.6%) of the participants were infected with single parasite and seventy four (64.3%) with multiple infections. Entamoeba histolytica 30.5% (n=77/252), Giardia lamblia 15.0% (n=38/252), Ascaris lumbricoides 17% (n=43/252), Trichuris trichura 11.1% (n=28/252), Enterobius v...
Saswati Roy1, Rakesh Pashi2, S. Dey3 and Shantanu Jha
... using the line transect method. Total 74 number of bird species were recorded which belonged to 18 orders. Out of 74 bird species 23 species (31.08%) were recorded as insectivorous, 16 species (21.62%) as omnivorous, 13 species (17.57%) as granivorous, 11 species (14.86%) as carnivorous, 7 species (9.45%) as frugivorous, 2 species were piscivorous (2.70%) and nectarivorous (2.70%). During the study, total fifteen species (20.27%) were recorded as local migrat...
Saleh S. Alhewairini
...s on finding appropriate methods for managing and controlling it. A huge fund was given for managing and controlling RPW in several countries. In this study, the potential toxicity of oxamyl to RPW was evaluated under both laboratory and field conditions. In the laboratory, oxamyl had significant effects on the mortality of RPW. After exposure to direct spray of oxamyl, adult mortality was 62, 82 and 100% whereas larvae mortality was 72, 77 and 100% after 1, 2...

SHARIF UDDIN, MD. HASIBUL HASAN, MOHAMMED MAHBUB IQBAL, MOHAMMAD AMZAD HOSSAIN

...uly by using gravimetric method where absolute fecundity during April was 16832.80± 673.34 and during July was 46186.14±2219.15. The relationship between gonad weight and fecundity, body weight and fecundity showed strong positive relation with regression co-efficient of 0.9603 and 0.9265 respectively which indicate that with the increase of body and gonad weight, fecundity was also increased. Microscopic observation ...
Sidra Iqbal1, Muhammad Irfan2*, Hafiz Abdullah Shakir1, Fouzia Tabbsum1, Javed Iqbal Qazi1
...sign of response surface methodology using three variables with three levels such as NaOH concentration (0.6, 0.8, 1.0%), substrate concentration (5, 10, 15%) and residence time (4, 6, 8h) with and without steam (autoclaving at 121oC for 15min and 15psi). After pretreatment, cellulase production was conducted in 250ml Erlenmeyer flask at 50 ºC, pH 5 with shaking speed of 120 rpm using 2% pretreated eucalyptus leaves as carbon source and 2% v/v inoculum si...

Hafiz Muhammad Tahir1, Khanum Zahra2, Arooj Zaheer2, Khizar Samiullah3

...ng to introduce advanced methods to manufacture spider silk artificially to enhance silk production. Silk fiber can be designed after determining type and sequence of constituent amino acid or by inserting silk producing gene in another suitable animal.

...

Niamat Ullah Khan1*, Abdul Aziz Khakwani2, Sami Ullah2, Najeeb Ullah2, Abdur Rauf3 and Inam Ullah2

...plicated thrice. Tillage methods were allotted to main plots while nitrogen levels were kept in sub plots. Results showed that RT produced higher bolls per plant, weight per bolls, lint yields, earliness % and benefit cost ratio (BCR) than FT. Ridge tillage gave lower percent of lodging. Results for nitrogen indicated that bolls numbers per plant, bolls weight, lint yield, earliness, lodging, BCR, fibre length and fineness were highest at 150-200 kg N/hectare....
Amjad Khan1,*, Muhammad Hassan Mushtaq1, Mansur ud Din Ahmad1, Jawad Nazir1, Zahida Fatima2, Asghar Khan3 and Shahid Hussain Farooqi3
...it and HI as serological methods for diagnosis, we found a 24.13% (168/696) overall seroprevalence, where the seroprevalence for H3N8 was 14.51% (101/696), for H7N7 6.03% (42/696), and 3.59% (25/696) as mixed infections, suggesting EIV current and active circulation in equine population of Pakistan. Statistical analysis suggested predicting variables including “local equine density per 2 KM”, “equines workings in ponds and rivers”, and ...
Nancy R. Howell
...has generated a fruitful method for and approach to Cosmic History.
...
...y instantaneous sampling method for 16 weeks, from 18th April to 8th July in 2014. The frequency of stereotypic behavior including auto mutilation, refusal of food stuff, feces licking, unnatural aggressiveness, pacing, head tossing was more periodic in lions housed at Lahore Zoo (site 1) in contrast to lions at display at Safari zoo Lahore (site 2). The frequency of natural behavior was periodic in African lions housed at Safari zoo Laho...
Abdul Malik1,2, Ghulam Abbas1,*, Abdul Ghaffar3, Sara Ferrando4, Lorenzo Gallus4 and Syed Sajjad A. Shah2,3
... basis by cultch removal method. Results showed that the highest fecundity, fertility, hatchability and survival of fry were obtained on salinity of 0%-15% and significantly decreased on 20‰ and 25‰. The eggs per gram body weight were also recorded in all treatments and highest eggs were obtained i.e. 4.0-4.3 per female on 0‰-15‰. Water temperature (28.02±0.12°C), dissolved oxygen (6.4±0.02 mg/L), pH (7.47&p...
Senol Celik1,* and Orhan Yilmaz2
...luation criteria of MARS method were R2=0.9193, Adj. R2=0.8983, r=0.9588, SD ratio=0.2840, RMSE=0.6041, RRMSE=3.2635 and ρ=1.6661. Taken together, the MARS algorithm appeared to be efficient compared to CART algorithm since the MARS algorithm’s goodness-of-fit criteria yielded better results. Using MARS algorithm, the body weight of animals (dogs) can be predicted and exploited in different performances.
...

Saima Akhtar Qureshi1 and Asim Anwar2*, Ather Maqsood Ahmed

...t the last resort in IMP method so it reduces the cost of production and increases the farmers’ profit. However, concerns about production may hinder widespread adoption of this technology by farmers. The aim of the study is to evaluate economic feasibility of IPM method in Punjab. The study consisted of 326 farmers (161 IPM producer and 165 NON-IPM producers) and compared input and output outcomes of IPM and NON-IPM f...
Mahboob Ali1, Muhammad Avais1, Riaz Hussain2, Kashif Prince1,*, Muhammad Ijaz1, Mamoona Chaudhry 3, Sehrish Firyal4, Amjad Islam Aqib1, Naimat Ullah Khan5 , Mian Saeed Sarwar5, Hazrat Ali5, Shahid Hussain Farooqi1, Ikramul Haq1 and Muhammad Sajid Hasni3
...tes using disk diffusion method. MDR were tested in vitro for response against various antibiotics. A questionnaire was filled for estimation of association of assumed risk factors with prevalence. Nonparametric tests at 5% probability were applied to check significance of results. The study noted overall 33.98% (123/362) of MDR S. aureus from subclinical mastitis milk samples while 123 of 192 S. aureus isolates (64.06%) were confirmed MDR...
Wali Khan1,*, Noor-Un-Nisa2 and Muhammad Asif Nawaz3
... smear and concentration methods. Two hundred and twenty one (21.2%) participants were found infected with one or more than one intestinal tapeworms. Seventy seven (7.39%) of the participants were infected with single parasite and one hundred forty four (13.8%) with multiple infections. Taenia saginata 32.6% (n=146/447), Ascaris lumbricoides 20.3% (n=91/447), Hymenolepis nana 19.7% (n=88/447), Trichuris trichura 14.3% (n=64/447),
Justyna Batkowska1,*, Lukasz Wlazlo2, Kamil Drabik1, Bozena Nowakowicz-Debek2, Karrar I.A. Al-Shammari3 and Magdalena Gryzinska1
...alternative disinfection method.
...

Saeeda Raza, Muhammad Naeem Safdar, Amer Mumtaz, Nouman Siddiqui, Khalid Nasim and Muhammad Amjad*

...s utilizing home cooking methods and evaluate the sensory attributes and shelf life at ambient conditions. Four different recipes of baby foods were developed containing 50% cereals, 30% legumes, 10% vegetables and 10% nuts and seeds. Different processing methods cleaning, washing, germination of pulses, peeling, cutting and blanching of vegetables, drying, roasting, grinding and mixing of ingredients were used. Recipe &ldqu...

 Z. A. Soomro, M.B. Kumbhar, A.S. Larik*, M. Imran** and S.A. Brohi***

...ough variance components method show that all the traits were highly heritable in F2 and F3 generations except sympodia/plant, indicating quantitative pattern of inheritance, offering better chances of selection for these traits in F2 and F3 generations. The traits namely plant height, bolls/plant and seed cotton yield/plant displayed high heritability (72.97 - 75.55%) with remarkable genetic advance (112.46 - 357.01%) indicating that these traits were predomi...

 Mohammad Siddique Munawar, Shazia Raja, Elizabeth Stephen Waghchoure* and Muhammad Barkat**

...treated with shook swarm method while other half were left without any treatment. The treated colonies showed significant difference from untreated colonies (t test, P< 0.005) and moribund colonies got recovered with increased bee population while the control colonies collapsed. It is therefore concluded that shook swarm method should be incorporated for prevention as well as curing of AFB.

...

 Abdullah Adil Ansari* and Kumar Sukhraj**

...x of 9}. This biological method of crop cultivation is sustainable and improves soil health rather than conventional methods based on the earlier observations.

...

 Ghulam Shabbir*, Muhammad Aftab, Abid Mahmood** Muhammad Kausar Nawaz Shah* and Nasir Mahmood Cheema***

...ried out by the pedigree method and the elite line was evaluated for its yield potential in various yield trials conducted at BARI, Chakwal from 1994 to 1998. Based on the desirable phenotypic traits and superior seed yield, SPS-5 was approved as a rapeseed variety “Chakwal Sarson” by the Punjab Seed Council in 2002 for general cultivation in the Punjab barani tract.

...

 Zaheeruddin Mirani*and Aslam Memon**

...rm visits and result and method demonstration methods of technology transfer were perceived as effective methods of technology transfer. Farmers were able to increase the yields of their farm produce. However, the picture says that there is yield gap between the potential yield obtained by research scientists and yield obtained by the farmers. Farmers were of the opinion that both public a...

 Naheed Akhtar, Waseem A. Gillani, Ehsan ul Haq, Javed Khan, Irshad Begum, Shaheena Yasmin* and Kamran Saeed**

...ne by seedling bulk test method for finding out resistant, moderately resistant and susceptible entries. The entry B-99036 was found to be resistant. Further studies were conducted to determine the mechanisms of resistance like antixenosis, antibiosis and tolerance. Results indicated that the entry B-00080 had antibiosis and B-99036 was the tolerant one. So, on the basis of present studies the entry having high level of resistance against R. padi (B-99036) sho...

Muhammad Zafarullah Khan1*, Rehmat Ullah1, Asif Nawaz1 and Ejaz Ashraf2 

...rough personal interview method. The results of this research study revealed that affinity toward Farm Services Centers were bulk of the educated, middle aged and small landholding farmers. Maximum of the respondents had above 20 years of farming experience. A rapid increase in the registration of farmers with Farm Services Centers was found in the last five years. Chi-square results indicated highly significant association (P≤0.05) between the duration of ...
Mustafa Sahin1,*, Esra Yavuz1 and Fatih Uckardes2
...ponents Regression (PCR) methods. For this reason, eggs were collected from 20 to 24 weeks of age, non-selected and random-mated Japanese quails. In this study, we investigated the characteristics of egg external quality such as egg width (X1), egg length (X2), Haugh unit (X3), and shape index (X4). The impact of these variables on albumen index was analyzed. Due to the multicollinearity between independent variables...

Imtiaz Hussain*, Hassnain Shah, M. Azeem Khan, Waqar Akhtar**, Abdul Majid*** and M. Yaqub Mujahid*

Corresponding author:izhussain@yahoo.com

PRODUCTIVITY IN RICE-WHEAT CROP ROTATION OF PUNJAB: AN APPLICATION OF TYPICAL FARM METHODOLOGY
...ng applying typical farm methodology. Rice is cultivated on 87% and fodder on 8% of the total area during kharif while wheat on 75% along with berseem on 14% of total area during rabi season. Almost 81% rice and more than 50 % wheat is harvested with combine followed by burning of residues that not only create environmental pollution but also affect the productivity of the cropping system. Stagnation in yield of rice and wheat is reported mainly due to uninter...

Akhlaq Ahmad, M. Anwar Arain*, Rahila Nazli**, Syed Anser Rizvi***,
Mubarik Ahmed* and Farzana Ibrahim****

Corresponding author: dr.akhlaqahmad@yahoo.com

COST EFFECTIVENESS OF UNDER-SHEET AND WHOLE GODOWN FUMIGATION OF BAGGED WHEAT
...t effective and feasible method for protection of stored cereals, and need to be implemented at the provincial and national levels.

 

...

 Saima Rani and Irum Raza*

COMPARISON OF TREND ANALYSIS AND DOUBLE EXPONENTIAL SMOOTHING METHODS FOR PRICE ESTIMATION OF MAJOR PULSES IN PAKISTAN
...out suitable forecasting method among the two forecasting methods namely trend analysis and double exponential smoothing. Measures of accuracy (MAPE, MAD and MSD) were used as the model selection criteria that could best describe the trend of prices of major pulses such as gram, mash, masoor and mung during 1975-76 and 2009-10. Double exponential smoothing method was found to be pertinent ...

 A.A. Mengal* , M. U. Mallah, Z. A. Mirani* and B. N. Siddiqui**

AN ANALYSIS OF PUBLIC AND PRIVATE AGRICULTURAL EXTENSION SERVICES IN BALOCHISTAN, PAKISTAN
...f did visit, the favored method of extension was by exhibition and seminar, which ranked 1st and nd 2 , respectively, based on the mean score for each extension teaching method used. A majority of the farmers received farm visits and field st demonstrations by private extension field staff, which were ranked 1 and nd 2 , respectively, as preferred methods of extension. Most of the farmers ...

 Amir Khatam*, Sher Muhammad, and Ijaz Ashraf**

ROLE OF INDIVIDUAL CONTACT METHODS IN DISSEMINATION OF AGRICULTURAL TECHNOLOGIES
...ts of individual contact methods in diffusion of agricultural technologies from 280 randomly selected farmer respondents. Researchers collected the data with the help of a pretested and validated interview schedule using survey technique. The results show that majority of the farmer respondents is aware of the farm visits (66.43%), demonstrations being conducted (62.50%) and home visits made by the extension staff (60.71%) as extension

 Amir Khatam*, Sher Muhammad and Ijaz Ashraf** 

ROLE OF FARMER FIELD SCHOOLS IN ENHANCING SKILLS OF FARMING COMMUNITY IN KHYBER PAKHTUNKHWA, PAKISTAN
...collected through survey method on various aspects from 280 randomly selected farmer respondents. The data collected were analyzed by applying simple descriptive statistics showing means, scores, ranking order and standard deviations. The results show that skills were highly enhanced in st the aspect of nursery raising techniques which was ranked 1 with mean value 3.40 closely followed by timely and balanced use of fertilizers and nd rd using recommended seed ...

 Muhammad Sohail*, Imtiaz Hussain*, Riaz-ud-din*, Syed Haider Abbas*, Maqsood Qamar* and Muhammad Noman*

EFFECT OF SPLIT N FERTILIZER APPLICATION ON PHYSIOAGRONOMIC TRAITS OF WHEAT (TRITICUM AESTIVUM L.) UNDER RAINFED CONDITIONS
...e N rate and application method to enhance wheat productivity. -1 Three N rates i.e., 60, 90, and 120 kg ha and three application methods i.e. full basal N dose at planting and N application in two and three equal split doses at tiller formation and stem elongation stages. Maximum grain yield -1 -1 (5.20 t ha ) was achieved when N was applied @120 kg ha in three equal split doses at planting, tiller formation and stem elonga...

 Sajida Taj*, Akhter Ali*, Nadeem Akmal*, Shujaat Yaqoob** and Mubarik Ali***

RAISED BED TECHNOLOGY FOR WHEAT CROP IN IRRIGATED AREAS OF PUNJAB, PAKISTAN
...odels to draw some solid method of irrigation with less wastage. Raised bed planting method is also one of the modern methods of planting crop with significant water saving. The study was planned and conducted by the Social Sciences Research Institute, Faisalabad in 2011-12 to assess the determinants of the adoption of the raised bed technology for wheat crop in irrigated Punjab, Pakistan....

 Naheed Zahra*, Muhammad Zubair Anwar*, Sonila Hassan** and Irfan Mehmood**

INSTITUTIONAL CREDIT ARRANGEMENT AND THEIR IMPLICATION ON AGRICULTURAL INCOME IN THE SELECTED VILLAGES OF RAWALPINDI DISTRICT
...inary Least Square (OLS) method was applied to generate the results. Results of this study revealed that there exists a positive and statistically significant relationship between institutional credit and agricultural income of the farmers. The other variables such as source of credit, ownership of farming machinery, access to market and family labour also have a positive and statistically significant relationship with the dependent variable except the age of ...

 Muhammad Shahzad*, Akhter Ali**, Abdul Hayee Qureshi**, Naveed Jehan*, Imran Ullah* and Majid Khan*

ASSESSMENT OF POST-HARVEST LOSSES OF PLUM IN SWAT, PAKISTAN
...mated through percentage method. The most important reasons were poor harvesting methods, over maturity, insufficient cold storage facility, poor grading, low-quality packaging materials and poor infrastructure. Total post harvest losses in the marketing channel of the plum were 21.51% of which 5.12% occurred at farm level, 1.44% at wholesale level, 6.31% at retail level, and 8.64% at the consumer level. Harvesting at proper...

Ayesha Tahir* and Sonila Hassan**

ECONOMIC FEASIBILITY OF SMALL SCALE BUTTON MUSHROOM PRODUCTION IN PAKISTAN
.... The cost of production methodology was used for this study. The yield and gross return of mushroom was estimated at 155.6 kg ha and Rs.77,800 ha , respectively. The results indicated the fact that mushroom production is very much remunerative to its producers as it can give maximum net return by reducing their cost of production as its cultivation is dependent on the agricultural raw material which is cheaply available.

...

 Amir Khatam*, Sher Muhammad**, Badar Naseem Siddiqui***, Muhammad Zakaria Yousuf Hassan****, Ijaz Ashraf**, Muhammad Zafarullah Khan***** and Aijaz Kuharoo******

COMMUNICATION OF AGRICULTURAL INFORMATION THROUGH GROUP CONTACT METHODS IN PAKISTAN
...on through group contact methods in Pakistan. Data were collected from 280 randomly selected farmers of four districts and analyzed using descriptive statistics. Survey method was used for data collection by researchers using a pre-tested research instrument. The results of the study show that sources of agricultural information used by the farmer respondents were seed/ fertilizer dealers, workshops, panel discussions, role ...

 Ali Ahsan Bajwa*, Ehsanullah*, Shakeel Ahmad Anjum*, Wahaj Nafees*, Mohsin Tanveer* and Hafiz Salman Saeed*

IMPACT OF FERTILIZER USE ON WEED MANAGEMENT IN CONSERVATION AGRICULTURE - A REVIEW
...e rate, timing, type and method of fertilizer application affect biological trends of weeds. In conservation tillage systems, the interaction between fertilizers and weeds is very complex, and there are reports of positive as well as negative correlations with weed establishment. Altered physical and chemical properties of reduced-tillage soils result in different weed growth and weed-crop competition periods and thus have indirect impacts on weed control stra...

 Asghar Ali*, Muhammad Tahir**, Shahid Riaz Malik* and Muhammad Hanif Munawwar*

EVALUATION OF PRE AND POST-EMERGENCE HERBICIDES FOR WEED MANAGEMENT IN LENTIL (LENS CULINARIS MEDIK.)
...control is the effective method of weed management. A field study was conducted to evaluate pre and post-emergence herbicides for weed control in lentil. The experiment comprised eight treatments including three herbicides, manual weeding and check (no weeding). The yield was higher in manual weeding but in herbicide treatments Isoproturon as pre-emergence @ 2 kg -1 ha produced statistically at par grain yield to that of manual weeding -1 followed by Isoprotur...

 Rehmat Ullah*, Kalim Ullah** and Mohammad Zafarullah Khan*

EXTENSION SERVICES AND TECHNOLOGY ADOPTION OF DATE PALM (PHOENIX DACTYLIFERA L.) IN DISTRICT DERA ISMAIL KHAN
...an by personal interview method from a sample of 51 respondents selected from these villages. Agriculture was the major profession of the farmers (80.3%). It was found that 62.7% of the respondents were never visited by the extension workers while 60.7% of the respondents never attended any meeting with field staff or Agriculture Officers. A single field day was organized by extension workers almost two years ago in the studied area attended by only 41% of the...

 Samiullah*, Mehmood Shah*, Kalim Ullah**, Rehmat Ullah*** and Ibrarullah****

PROFITABILITY OF WHEAT PRODUCTION IN DERA ISMAIL KHAN
...May-June, 2012. A survey method was employed and 11 villages were selected at random which are the major wheat producing villages in the said district. A total of 50 respondents were probed by personal interview method from the sampled villages. Cost and return (gross return) data from the respondents were analyzed. Farm budgeting technique for estimating cost, gross return and profitability was applied. The analysis of data...

 Aniqa Iram*, Javed Khan**, Nadeem Aslam**, Ehsan-ul-Haq**, Habib Iqbal Javed**, Muhammad Irfan*, Awais Rasool*, Muhammad Ishaque Mastoi** and Sumera Aslam* 

EFFICACY OF PLANT DERIVED OILS AND EXTRACTS AGAINST WHITEFLY, BEMISIA TABACI (GENNADIUS) ON SESAME CROP
...nts worldwide. Different methods are being used for its control. The present experiment was conducted to determine the effect of some plant extracts of mint (Mentha spp.) and geranium (Pelargonium graveolens) and soybean oil (Glycine max), mustard oil (Brassica spp.) and taramera oil (Eruca sativa) against whitefly, Bemisia tabaci on sesame crop. The data were recorded 24h before and 24h, 48h, 72h and 168h after application of each spray material. The results ...

 Muhammad Shahzad*, Ayesha Tahir**, Naveed Jehan*** and Muhammad Luqman*

IMPACT OF DIFFERENT PACKAGING TECHNOLOGIES ON POSTHARVEST LOSSES OF STONE FRUITS IN SWAT PAKISTAN
...mated through percentage method while partial losses were estimated at the wholesale and retail level by price differential method. Multiple regression analysis was employed to find relation between post-harvest losses and different factors at three different stages. Findings of the study revealed the channel of cardboard box technology accounted for post-harvest losses of 10.49% while at farm level, losses were 2.90%, at wh...

 Syeda Aimen Hadi*, Abid Ghafoor Chaudhry*, Aftab Ahmed** and Shaheer Ellahi Khan***

PERCEPTION, APPROACHES AND PRACTICES OF LOCAL FARMING AND DEVELOPMENT: AN ANTHROPOLOGICAL APPROACH
... nexus of modern farming methods, due to which they are being abandoned by the farmers. Modern farming which is representative of development has increased the competition, transitioning, breaking the community into people experimenting on modern technology and those who are still finding means to adapt their traditional agriculture to the fast changing needs of the societies. The research was carried out in the villages of Ghora Gali and Aruka through qualita...

 Riaz Hussain*, Muhammad Riaz** and Mushtaq A. Saleem***

TOXICITIES OF AZADIRACHTIN AND POLYCHLORINATED PETROLEUM HYDROCARBON AGAINST RESISTANT AND SUSCEPTIBLE STRAINS OF TRIBOLIUM CASTANEUM (COLEOPTERA: TENEBRIONIDAE) ADULTS
...filter paper impregnated method against azadirachtin (Nimbokil 60 EC) and polychlorinated petroleum hydrocarbon (Tenekil 100 EC). The LC values of these insecticides were worked out as 12830 and 50 9331 ppm for azadirachtin and 5148 and 4047 ppm for Tenekil 100 EC against PAK and FSS-II strains, respectively. The results revealed that polychlorinated petroleum hydrocarbon was more toxic than the azadirachtin. Furthermore, both the insecticides were equally tox...

 Muhammad Asif Jamil*, Nowshad Khan*, FarhatUllah Khan* and Muhammad Zakria*

EFFECTIVENESS OF AGRICULTURAL EXTENSION SERVICES IN DISSEMINATION OF INFORMATION TO THE FARMING COMMUNITY OF DISTRICT BHIMBER, AZAD JAMMU AND KASHMIR
...l as multistage sampling method was used to collect the data from the study area. The overall data consists of 150 farmers. The study revealed that respondent's acquaintance with extension staff was very low as only 18.00% of the respondents had personal acquaintance with the extension staff and 12.67% of them knew extension staff through their neighbour, while 38% of respondents from their friends. The farmers who knew extension staff through relatives were o...

 Sobia Naheed*, Irum Raza*, M. Zubair Anwar*, Nusrat Habib*, Naheed Zahra* and Sabeen Siddiqui*

FORECASTING AREA AND PRODUCTION OF BARLEY IN PUNJAB, PAKISTAN
...le exponential smoothing method, therefore, this method was selected for predicting barley production and area in Punjab from 2012 to 2016. Five years forecast for barley area in Punjab was 26.140,
25.360, 24.580, 23.800, 23.020 ha and forecast of barley production in Punjab was 24.620, 23.950, 23.270, 22.600 and 21.930 tonnes for 2012, 2013, 2014, 2015 and 2016, respectively, with a 95% prediction interval. Decl...

 Zafar Abbas*, Ijaz Ahmad**, Adnan Shakeel**, Muhammad Abdullah***, Muhammad Islam**, Sadiq Muhammad*, Ghulam Murtaza* and Mushtaq Ahmad**

EFFECT OF PHOSPHORUS FERTILIZER AND WATER STRESS ON PROTEIN AND PHENOLIC CONTENTS IN COTTON (GOSSYPIUM HIRSUTUM L.)
...ys of planting. Kjeldahl method and thin layer chromatography (TLC) were used for the quantitative and qualitative analysis of total protein and phenolic compounds, respectively. Proteins were greatly affected by fertilizer treatment and water stress, but phenolic compounds remained unchanged upon fertilizer treatment. However, they were greatly affected by irrigation and water -1 stress. Crop treated with 100 kg ha P O under water stress maintained high 2 5 p...

 Azhar Usman Ali*, Ghulam Sarwar*, Muhammad Aftab* and Sher Muhammad**

EFFECT OF SOIL AND FOLIAR APPLIED COPPER ON GROWTH AND YIELD OF WHEAT (TRITICUM AESTIVUM L.)
...n by using wet digestion method. Results of the experiment indicated that soil application of copper proved better than foliar application in increasing the yield and yield components of wheat. Similarly, foliar application -1 -1 of copper at 4mg kg (T ; level 2) was better than 2 mg kg (T ; level 1). 

...

 Abdul Razaq*, Muhammad Jamal Khan*, Tahir Sarwar* and Mohammad Jamal Khan**

EFFECT OF DEFICIT IRRIGATION, SOWING METHODS AND MULCHING ON WHEAT YIELD AND NITROGEN UPTAKE
...ere studied i.e., sowing methods, mulching and deficit irrigation with four levels viz: full irrigation (DI ), 20% deficit irrigation (DI ), 40% deficit irrigation (DI ) and 60% deficit irrigation (DI ). Soil moisture was continuously monitored and full irrigation was determined on the basis of 50% Management Allowed Deficit (MAD). Results indicated that sowing methods, mulching and various levels of deficit irrigation signi...

 Dilawar Khan*, Abid Saeed*, Junaid Ahmad*, Imtiaz Qamar**, Fazal Yazdan**, Saleem ud Din** and Muhammad Tariq**

ASSESSMENT OF RIPARIAN VEGETATION IN DHRABI WATERSHED AND CHAKWAL REGION IN PAKISTAN
...g line transect sampling method. On the basis of top three highest IV plant community were identified at western side of the Kallar Kahar Lake while plant community was observed in eastern side by using 1x1 m quadrat method.

...

 Ishaq Ahmad*, Abdul Rehman**, Ehsan –Ul- Haq*** and Arif Mahmood****

RESISTANCE RESPONSE OF RICE ( L.) GERMPLASM AGAINST RICE LEAF FOLDER ( G.) UNDER GREENHOUSE CONDITION IN PAKISTAN
... effective and long term method as compared to any other control measure. It was imperative to exploit the sources of resistance to cope with threat the pest and its environmental friendly control. Present study was carried out in greenhouse of Plant Genetic Resources Institute (PGRI), NARC. Twenty six Pakistani genotypes of rice germplasm including 15 coarse and 10 fine genotypes were screened in pot experiment during 2011 including TNI, used as susceptible c...

 Hina Fatima** and Bushra Yasmin**

EFFICIENCY AND PRODUCTIVITY ANALYSIS OF PAKISTAN'S FARM SECTOR: A META-ANALYSIS*
...al information regarding methodological, regional and crop specific influences on farm sector of Pakistan. The data rely on the published research papers on efficiency analysis of Pakistan's farm sector from 1971 to 2014. Three models separately estimated included methodological model, crop specific model and provincial model. These models incorporated the impact of methodological variable...

  Mohammad Umar Farooq* , Sarfraz Ahmad ** , Mohammad Islam and Imtiaz Ahmad Qamar ***

ASSESSING THE STATUS OF CARBON POOL IN TWO RANGELAND GRASSES ( ) IN POTHWAR, REGION, PAKISTAN
...stion and Dry Combustion method. Four transect lines were drawn in each experimental plot. Twenty four samples of each experimental plot were collected with the help of ADC one m quadrat method, weighed and then oven dried at 60 C to find out the dry weight for above phytomass carbon Mg C ha . Similarly, the one m quadrats were dig out up to the root level for each grass and separated the root portion and weighed, oven dried...

 Ehsanullah*, Muhammad Amjad Shahzad*, Shakeel Ahmad Anjum*, Ali Zohaib*, Muhammad Ishfaq* and Ejaz Ahmad Warraich*

EFFECT OF DIFFERENT SOWING METHODS AND PLANTING DENSITIES ON GROWTH, YIELD, FIBER QUALITY AND ECONOMIC EFFICACY OF COTTON
...r under different sowing methods and planting densities. A field experiment was conducted to evaluate the influence of different sowing methods and planting densities on growth, yield, quality and economic returns of cotton. Sowing methods included pit planting (1 m × 1 m pits), bed planting (75 cm apart beds), ridge planting (75 cm apart ridges) and line sowing with varied inter row...

Hina Zubair1, Saima Perveen2, Saeed Alam3, Akkad Rafiq4* and Sara Ejaz 

...ifferent family planning methods in relation with socio-demographic factors at divisional headquarter hospital in a year time. A retrospective study was performed by analyzing one year records from family planning clinic at divisional headquarter hospital. At family planning centers, a total of 920 individuals attended the Reproductive Health Services from April 2015 to March 2016. Among them, 92% individual utilized temporary method

Samina Ashraf1*, Ghulam Haider2 and Maimoona Ashraf

...henomenological research methodology has been used to explore, interpret, and evaluate the personal narratives of women with disabilities about their experiences with violence and abuse. The phenomenology approach was used to study the phenomena. A number of 05 women with disabilities who had physical disability or sensory impairment (visual and hearing) were purposively selected as the participants of the study. The age of participants was between 25 to 40 ye...
Selçuk Kaplan1,* and Sertaç Atalay2
...ment length polymorphism method. The single nucleotide polymorphism (SNP) in the regulatory region of the IGF1 gene was detected by amplification of the 294 bp region using specific primers and cleavage with the BfoI enzyme. Allele frequencies of A and B were found with 0.915 and 0.085 respectively. The genotype frequencies of IGF1 gene were 0.85 (AA), 0.13 (AB) and 0.02 (BB). The SNP in the exon 3 of the LEP gene was detected by am...
Xiaopeng Tang1,3, Rong Xiang1, Sijia Chen1,3, Shufen Yang1,3, Hu Liu1,3, Rejun Fang1,3,* and Aike Li2,
...and enzymatic hydrolysis method offer effective approach to improving the quality of unconventional protein sources, such as the CSM.
...
Burcu Onuk1,*, Murat Kabak1, Bünyamin Sahin2 and Nazan Gezer İnce3

 

...ple of the stereological methods. The volume and volume fractions of structures were assessed. Results obtained from the CT images were compared between stork and seagull. The volume fractions of the conchae, nasal cavity and nasal septum were 30.24%, 55.22%, 14.54%, and 33.03%, 53.23%, 13.73%, in stork and seagulls, respectively. The volume fractions of structures did not show statistical difference between two species. Our findings suggest that the nasal str...
Abdul Nabi Jatt1,*, Sarfraz Ali Tunio1, Shaista Bano Memon1, Abdul Sattar Qureshi2 and Muhammad Aqeel Bhutto
...ive to recent biological methods. This method could be applied for the identification of various enzymes on routine basis directly in bacterial cultures and biological fluids.
...
Grace Chinenye Onyishi, Ifeanyi Oscar Aguzie, Joseph O. Okoro, Christopher Didigwu Nwani*, Ngozi Ezenwaji, Ndubisi Stanley Oluah and Fabian C. Okafor
...ities. Light and teasing methods were used for recovery of parasites from the snails. A total of 618 snails belonging to 7 species (Achatina achatina, A. belteata, A. degneri, Limicolaria aurora, L. flammea, Lamellaxis gracilis and Orthalicus sp.) were collected. A. achatina was the most abundant species (35.6%) while A. degneri was the least (1.3%). Overall, 388 snails (62.8%) were infected. A. achatina had the highest (76.4...

Ejaz Ashraf1*, Hafiz Khuram Sharjeel1, Raheel Babar2, Muhammad Junaid1, Qamar Iqbal3, Rabia Rasheed1 and Nosheen Fatima1 

...rogram and demonstration methods were dominant approaches used in technology transfer. The mean of the results showed that low allocation of budget, massive operational area to give satisfactory coverage, less use of multimedia, unavailability of training facilities to EFS, manifold duties assigned to extension officers and top-down dimension of planning of implementation of extension approaches were main weaknesses. The steps like effective evaluation of exte...
Yan Li1, Kai Zhan1,*, Junying Li1, Wei Liu1, Ruiyu Ma1, Shengnan Liu1, Tao Han2, Shaoquan Li2, Shaolin Wang2 and Yang Hu2
...de to choice of breeding methods for poultry enterprises.
...

Osama Elshazly1, AbdelSatar Arafa1, Mohammed A. Rohaim2, Ismaeil M. Reda2 and Hussein A. Hussein2*

... and molecular detection methods. Full-length hemagglutinin (HA) sequences of six representative H5N1 isolates were analysed to study their genetic evolution followed by estimation of their evolutionary rates among different virus clusters. This analysis revealed a high evolution rate for clade 2.2.1.2. Additionally, analysis of selection pressures in the HA gene revealed a positive selection pressure. Deduced amino acid analysis revealed characteristic mutati...
Waqas Ali1,*, Arshad Javid1, Syed Mohsin Bhukhari1, Ali Hussain1, Syed Makhdoom Hussain2, Hira Rafique1
...tive effects of sampling methods used on habitat and taxa under consideration. In addition, study area must be sub-divided into small patches and more than two suitable sampling techniques should be used to record all possible species.
...
Noor Fatima1, Muhammad Irfan2,*, Hafiz Abdullah Shakir1, Javed Iqbal Qazi1
...through response surface methodology. Pretreatment conditions were optimized using three variable with three levels like sulfuric acid concentrations (0.6%, 0.8%, 1% v/v), biomass loading (5%, 10%, 15% w/v), and residence time (4, 6 and 8h). After pretreatment process, cellulase production was achieved by solid substrate which was conducted in 250mL capacity Erlenmeyer flask in which incubation of Bacillus subtilis was carried out for 24 h of fermentati...
Archana Naithani, Pongthep Suwanwaree* and Bartosz Nadolski
...ys following point count method during November 2016 to April 2017 in the campus of Suranaree University of Technology, Nakhon Ratchasima province, resulted in the identification of 94 bird species belonging to 12 orders and 35 families. Altogether 3,328 individuals were observed. Out of 35 Families, Sturnidae and Sylviidae hadthe highest number of species (5 species each).Six simple dietary guilds were observed during the present study; the maximum number of ...

Mohammad Javad Golmohammadi1, Hamid Reza Mohammaddoust Chamanabad1*, Bijan Yaghoubi2 and Mostafa Oveisi

... each year) based on the method of Thomas by throwing 0.5m × 0.5m quadrat to count existing genus and species of weeds. According to the results, the highest frequency was related to weed species of Echinochloa crusgalli (96.9%), Paspalum distichum (78.8%) and Eclipta prostrate (51.8%). Cyperaceae and Poaceae families showed the highest frequency with 13 species (20%) and 8 species (12%), respectively. The RC of E. crusgalli had the highest dominance (...
Jiji Li*, Tianyang Zu, Xiangli Dong, Xiao Yang, Wei Liu and Changwen Wu
...gs could help to develop methods of disease resistance in the aquaculture industry.
...

Baharullah Khattak1*, Saifullah2, Shaukat Hussain2, Musharraf Ahmad2, Asad Ali2, Mohammad Junaid2, Ijaz Ahmad Khan3, Taj Ali Khan1 and Mubbashir Hussain1 

Aqeela Ashraf1,2,*, Muhammad Imran1 and Yung-Fu Chang2
...formed by disk diffusion method. Resistance and virulence genes were detected by PCR. The results showed that 100% of isolates were resistant to penicillin, 54% to ampicillin, 44% to tetracycline and 30% to streptomycin, while none of them was resistant to chloramphenicol. These E. coli isolates carried one or more antimicrobial resistance genes. Genes present with highest frequency were tetA (42%), tetB (28%) and ampC (26%). Fewer ...
Niamatullah Kakar, Farhat Abbas, Muhammad Shafee and Tauseef Asmat*
...st while other detection methods were not that sensitive to detect the tuberculosis at that stage. Two patients were reported with history of tuberculosis but were effectively treated before imprisonment. On age basis, 128/186 (68.88%) patients were of younger age (18-30 years) and 10 (5.37 %) were above 50 year. Illiteracy, unemployment (daily wages) and tobacco smoking were some of the main risk factors common in inmates. The trend of sharing blankets, towel...
Baoying Guo*1, Yu Chen1, Chuan Zhang2, Zhenming Lv1, Kaida Xu3, Hongling Ping4 and Huilai Shi4
... likelihood and Bayesian methods were used to build phylogenetic trees based on protein coding mtDNA genes of 37 cuttlefish species. S. lycidas have a close relationship with S. pharaonis, S. aculeata, and S. esculenta. This result confirmed the relationships of S. lycidas as being similar to the traditional taxonomy. This study plays an important role in the investigation of phylogenetic relationships, taxonomic resolution, ...
Mingming Ning1,2, Yanjie Zheng1, Yuanyuan Dun1, Weijun Guan2 and Xiuxia Li1,*
...he isolation and culture methods of chicken amniotic fluid stem cells (CAFSCs) in vitro, characterizing CAFSCs and inducing CAFSCs to differentiate into three layers. Methods and results include that more CAFSCs are gained via thermoelectric method with lower cost. Groping and optimizing culture system abiding by the idea of simulating the chicken body environment, involving adding ...
Danyu Zhang1, Ying Liu1, Qinxin Shi1, Zhiwei Peng1, Yan Hua1 and Zhijun Hou1,2,*
...ting and next sequencing methods. The parasites were Capillaria sp., Baylisascaris devosi, Echinochasmus sp., and two Coccidianspecies. The Echinochasmus sp. andtwo Coccidian species were first time found either in sable or in other martens. The 434 genus of bacteria, belong to 30 phylum, the more popular bacteria were Proteobacteria (33.54%), following by Firmicutes (18.58), Acidobacteria (12.82%)...
Bin Wang1, 2, Qianji Ning1,*, Qian Wang2, Wei Peng2, Tong Hao2,*and Jinsheng Sun2,*
... bioinformatics analysis method, which accounts for 91.9% of all un-localized proteins in the network. Subsequently, the molting interaction network was extracted from the global PPI, which contains 35 proteins and 32 interactions. By analyzing the subcellular annotation of the molting interaction network, the subcellular localization of 12 proteins was obtained. Half of these proteins located on the cell membrane, indicating the close relationship between mol...
Xi-wen Chen1,2, Qian Wang1,3, Miao Yin1, Zhong-hui Pu1,2, Ai-wei Guo3, Lian Li 1,3, Wen-tao Luo1,3 and Xiong-qing Wang1,*
...Green I dye. The RT-LAMP method was approximately 100-fold more sensitive than RT-PCR for PRRSV detection. The assay was also specific for PRRSV and did not cross react with CSFV, PCV-2, PPV or PRV. In clinical samples (n=5) RT-PCR and RT-LAMP both identified the same 2 PRRSV infected samples. Thus, the novel RT-LAMP assay described here is a rapid, simple, sensitive, specific test for PRRSV that can potentially be applied in clinical settings.
...
Muhammad Shahid Saeed1, Adeela Shahid2, Samia Jawed3, Muhammad Akram4, Irshad Hussain Qureshi5 
Sadaf Noveen1*, Shaista Habib Ullah2, Babar Alam3
Nasreen Ishaq1, Arif Rasheed Malik2, Zameer Ahmad1, Saad Ehsan Ullah3
...r detection of different methods of identification to establish a baseline of identity e.g. dental data, fingerprinting, DNA analysis, anthropometry, identification of sex, assessment of age, determination of height and blood groups identification. Among these, DNA analysis and dental data provide easiest identifications, however, these techniques are expensive and not readily available necessitating additional techniques for identification. One of such novel ...
Masood Nizam Tabassum1*, Muhammad Ather Khan2, Saira Afzal3, Amir Gilani4, Abdul Wahab Gureja5, ShafaqTabassum6
Yan Li1, Kai Zhan1,*, Junying Li1, Wei Liu1, Ruiyu Ma1, Shengnan Liu1, Haijun Cui1 and Xin Zhang2
...cks was performed by the method of partial amputation of the third toe (along the distal phalanx) at day of hatch; the first and third toes were declawed in the group 3, while the second and fourth ones were trimming in group 4. They were caged with the same batch of non-declawed hens. Compared with intact control, laying rate was significantly increased in groups 3 and 4, while percentage of defective eggs was visibly decreased in group 3. Hatching experiment...

Saeed Ullah1, Amanullah Jan1, Murad Ali2*, Wiqar Ahmad3, Hafeez Ur Rehman2, Muhammad Ishaq2 and Bilal Ahmad2  

...">The time, quantity and method of nutrients application are crucial factors for enhanced crop yield and economic return. This study investigated the effect of phosphorous (0, 35, 70 and 105 kg P2O5 ha-1) and zinc (0, 10, 20 and 30 kg ha-1) levels applied through broadcast and banding on chickpea growth and yield during winter 2013-14 at the New Developmental Farm, The University of Agriculture, Peshawar. The experiment was carried out by following randomized ...
Amna Waseem, Sikander Ali* and Syeda Wajiha Khalid
...um sulfate precipitation method at 40 %. The molecular weight of the enzyme was found to be 33 kDa. The results showed that the enzyme produced from mutant strain gave significantly higher (p≤0.05) lipase activity than the wild-type. From the results presented in the study, it was observed that maximum lipase activity could effectively be achieved after EMS and MMS-induced mutagenesis of filamentous fungus culture used.
...
Muhammad Shaheer1*, Asad Aslam Khan2, Nasir Ahmed3, Tehseen Mahmood Mahju4, Ummarah Rasheed5
Maaz Ahmad1*, Mussab Ahmad2 and Tehreem Munir3
Taimoor Akram Khan1*, Anam Farooq2, Amna Farooq3, Muhammad Nasir1, Gohar Khan4 and Ali Akram Khan1
Rana Khurram Aftab1*, Farhat Ijaz2, Samia Jawed3
Muhammad Kashif Munir1*, Sana Rehman1, Rizwan Iqbal1, Muhammad Saqib Saeed2, Muhammad Aasim1
...standard drug proportion method. Additionally, to explore whether the assay can be utilized in treatment of Multidrug Resistant TB
Settings: This study was undertaken in Pakistan Health Research Council TB Research Centre in collaboration with Department of Pulmonology, King Edward Medical University, Mayo Hospital, Lahore.
Methods: Sputum samples from 125 patients were collected from co...
Sahar Naz1, Farhan Rasheed2, Muhammad Saeed3*, Shagufta Iram4 and Ambereen Anwar Imran5
...irby Baur disc diffusion method. Zone sizes were interpreted according to CLSI 2016 guidelines. Next day every carbapenem-resistant isolate were further processed for MβL detection by EDTA method, zone size of Carbapenem disc only and Carbapenem disc impregnated with EDTA was compared ( >7 mm increase MβL positive, 0-5 mm increase MβL-negative).
Results: Out of total 12126 samples, 35.9% ...
Munir Ahmad1, Aisha Hameed2*, Saba Khaliq3, Hamid Saeed Malik4 and Shahida Mohsin5
Faisal Masood*1, Ranjit Kumar Sah2 and Ahmad Humayun Sarfaraz3
Naveed Rashid*1, Muhammad Aamer2, Uzma Malik3, Nimra Arif4, Muhammad Waqar Akram5 and Ayesha Irshad6
Muhammad Younus1*, Sabah Usman2 and Samia Jawed3
Maryam Javed*, Farwa Saghir, Naveera Aziz, Maham Saeed, Asif Nadeem and Wasim Shehzad
...set. Then Sanger’s method of DNA sequencing was used. Multiple sequence alignment demonstrated that sheep and goat were having the wild base Guanine against which the cattle and buffalo depicted the mutant form Adenine. Results of this study illustrated the genetic tendency of large domestic bovines towards aggression and attack, so they should be handled with great caution.
...
Ayesha Aihetasham, Saira Shariq and Javed Iqbal Qazi*
...mploying surface culture method. Following different time exposures, R. stolonifer significantly produced epozootics on the tested insects, with resultant decrease in food consumption. Behavioral changes included lethargic workers which were observed even being eaten up by other workers. These experiments revealed that R. stolonifer is a potent biological control agent against H. indicola. Scaleable cultivation of this fungal species and i...
Muhammad Sarfraz*, Shahida Hasnain
...bitor based confirmatory method. Antibiogram was performed following CLSI guidelines 2013. Of 11,725 clinical specimens, 29 % were culture-positive. 80% K. pneumoniae and 70% E. coli showed resistance to cefotaxime or ceftazidime. Only63% K. pneumoniae and 58% E. coli and were resistant (<18 mm) to FOX. Phenotypic confirmation of pAmpC beta-lactamases was done using inhibitory based method

Muhammad Khalid1,2 and Muhammad Naeem2* 

...don idella. Standardized methods were used to determine the proximate composition of 72 samples. On wet weight basis, mean percentage for water was 80.76 %, ash 3.40 %, 4.31 % for fat and 11.53 % for protein in C. idella. Percent water contents showed inverse relationship with % protein (dry and wet body weight), % ash and % organic contents (wet body weight). Wet body weight (W) showed negative correlation (P < 0.001) with percent water, percent fat (dry a...
Wei Meng1,3, Tianyan Yang2,*, Yunguo Liu1, Mahmut Halik1 and Tianxiang Gao2
...cted by Neighbor-Joining method to reveal the evolutionary relationships within subfamily Schizothoracinae. Three different grades of schizothoracine fishes were well recognized from each other in branching diagram. The primitive group and the specialized group + the highly specialized group constituted a sister relationship with strong supports.
...

Raphael Olanrewaju Babatunde, Adeyemi Esther Omoniwa and Miriam Ukemenam 

...he Ordinary Least Square method. The result showed an overall gini-coefficient for gender inequality in schooling of 0.35, with the value for boys and girls at 0.35 and 0.49 respectively. Mother’s education, age of the household heads, marital status, dependency ratio, farm size and the average time spent by the children on the farm were found to significantly influence children’s access to schooling. While increase in schooling cost, average years...

Ansaar Ahmed1, Imtiaz Hussain2*, Ibni Amin Khalil3, Subhanulla3, Gulzar Ahmed3, Imtiaz Ahmad3 and Muhammad Imtiaz2  

...ify;">Crop establishment methods like broadcasting of wheat and manual planting of maize on flat surface coupled with flood irrigation are used in irrigated maize–wheat system of Khyber Pakhtunkhwa (KP) province that requires skilled labor, intensive tillage and more water for irrigation. In this study, zero tillage and bed planting effect in comparison with farmer practice on maize-wheat system productivity was evaluated at five sites in district of Now...
Safia Rehman1, Nazish Mazhar Ali1,*, Gerald B. Pier2, Iram Liaqat1 and Bushra Mazhar1
...ment of chemotherapeutic methods against multidrug resistant bacterial pathogens in chronic and acute lung infections. It provides a practical approach towards the use of nanoparticles to enhance antimicrobial activity against these pathogens.
...
Arifa Mehreen1, Iram Liaqat2,Muhammad Arshad3, Muzzamil Waheed4 and Najma Arshad1,*
...biological and molecular methods. The agar disc diffusion method was used to evaluate the antibiotic susceptibility. Virulence-associated genes were detected using PCR while association analysis was used to test the likelihood of strains carrying combinations of genes involved in toxin production and/or host immune evasion. Highest resistance was observed against beta-lactam group followed by cephalosporin, lincosamide, tetr...

Ejaz Ashraf1*, Hafiz Khurram Shurjeel2 and Mujahid Iqbal3  

...or comparison of the two methods of instructions. The results of the study revealed that both methods were equally applicable and effective regarding timely transfer of information or knowledge to the farmers for aphid attack on wheat crop. Hence, it is concluded that the goal for creating awareness among farmers for the use of Mobile phone cellular technology is as effective as face-to-face (traditional
Rida Zainab1, Sana Elahi1, Afshan Kaleem2,*, Daniel C. Hoessli3, Mehwish Iqtedar2, Roheena Abdullah2, Faiza Saleem2, Shanza Khan1, Anusha Ijaz1, Shagufta Naz2 and Abdul R. Shakoori4,5,*
...One of the computational methods becoming increasingly popular to develop new drugs is relying on Pharmacophores. This method was utilized to develop pharmacophore models of Akt2 inhibitors and β2-Adrenoceptor agonists. A pharmacophore model is proposed, using fourteen second generation and one first generation antipsychotic drugs for Schizophrenia that are effective against both 5-HT2a and D2 receptors. Hydrogen bond a...
Junaid Akhtar1,2, Sidrah Saleem1, Naveed Shahzad3, Abdul Waheed1, Iqra Jameel1, Farhan Rasheed4 and Shah Jahan5,*
...ed disks diffusion (CDD) method, respectively. The MBL producing clinical isolates were further subjected to PCR for the existence of blaIMP and blaVIM genes. The carbapenem resistant A. baumannii (n=32), P.aeruginosa (n=26), K. pneumoniae (n=19), E. coli (n=16), C. ferundi (n=04), P. vulgaris (n=02) and E. cloacae (n=01), were isolated from clinical samples of hospitalized pa...

Mian Noor Hussain Asghar Ali, Liaquat Ali Jamali, Shakeel Ahmed Soomro*, Shakeel Hussain Chattha, Khalil Ahmed Ibupoto, Naseer Ahmed Abbasi and Noor Mehdi Qumi  

...days under two different methods i.e. one kept open and other covered with sugarcane trash. The samples of sugarcane were taken at an interval of 24 hours to determine different quality parameters i.e. cane weight, brix, pol/sucrose, purity, fiber and commercial cane sugar. The results revealed that weight loss and fibre content throughout increased with increasing storage period. However, a decrease was observed with increasing storage period for brix content...
Yang Liu1,2,3, Baimei Liu1,2,3, Chenchen Li1,2,3 and Meiwen An1,2,3,*
...ed using Q-PCR and ELISA method, respectively. Co-culture KM&FM under 3.4 kPa pressure enhanced typeIcollagen and type III collagen mRNA expression and protein secretion from KM. It however reduced typeIcollagen and type III collagen mRNA expression and protein secretion from FM; it promoted mRNA expression and protein secretion of IL-1α from KM and enhanced expression of MMP-3 from FM. The MMP-3 mRNA expression and protein secretion from FM were res...

Sajad Ali1*, Syed Asghar Ali Shah2, Jaffar Ali3, Muhammad Nadeem Iqbal4, Arshadullah Jadoon5 and Farmanullah

...nario of socio-economic, methodical and marketing restraints faced by the farmers in augmenting and stimulating export of the country. The pea’s cultivators and also market agents were quizzed by using structured questionnaires in two related districts that is Haripur and Mansehra in the year 2013-14. The data were analyzed by using SPSS Package version 22 for calculating marketing marginal analysis and cross-tabulation were also been employed. The studi...
Nimra Naseer, Adeela Fatima and Imran Sajid*
...ical screening different methods including cross streak, agar plug and well diffusion method were performed. Seven Streptomyces strains including NWMD-6, NWMD-7, NWMD-10, NWMD-11, NWMD-12, NWMD-15 and NWMD-16 were found to have significant antimicrobial activity against MDR bacterial pathogens. Secondary metabolite profiling of each methanolic extract by TLC and HPLC-UV signified the presence of unique and diverse met...

Muhammad Israr1*, Saeed Ullah1, Shakeel Ahmad2, Asif Yaseen3, Urooba Pervaiz4 and Nafees Ahmad5 

...to face interview survey method by filling a questionnaire. Farmer’s perceptions on agriculture LD were measured through the extent of severity scale in terms of LD dimensions and indicators resulted from water, tillage, soil, salinization, and overgrazing. Moderate (0.49) index of water degradation and the changing climatological index were light but the reduction of drainage density of water runoff and slope gradient reduction of soil index was severe....

Asif Nawaz* and Muhammad Zafarullah Khan 

...onal allocation sampling method. Data from the respondents were collected through pretested and validated interview schedule. Data was analyzed using SPSS Ver.20. Mean, standard deviation, chi-square test and paired sample t-tests were calculated to achieve the study objectives. Results of the study revealed that Field Assistants were incompetent in competencies regarding record keeping on climate change issues, recording and reporting climate change impacts, ...

Aneel Salman1, Muhammad Iftikhar ul Husnain1, Inayatullah Jan2*, Muhammad Ashfaq2, Mudassar Rashid1 and Usman Shakoor1 

...ing, and drip irrigation methods are prevalent in the research area. The major hurdles that farmers perceived in adaptation are limited access to credit, lack of access to information, and institutional support. As expected, no significant variations were found across districts in the characteristics of respondents (age and income) that can affect their perceptions, their adaptation strategies, and obstacles faced in adaptation. The results of the study need t...
Mehwish Aftab, Tariq Mahmud*, Muhammad Asim Raza Basra, Aqsa Gulzar, Sumaira Basharat and H.M. Junaid
...us by disc diffusion method and antifungal activity against Macrophomina phaseolina, Fusarium oxysporum. The metal (III) complexes are highly active under permissible limits.
...
Ashraf M. Mounir, A.N. El shahat* and A.M. Abdul Azeem
... be used as an efficient method for sterilization and increasing the active contents of frankincense. Also, gamma-irradiated frankincense can be used as an effective cardio protective natural agent in MI.
...

Asma Sohail1*, Aina Rafique2, Kashif Sarfraz Abbasi1 and Maryum Arif3 

...tivity. Effect of drying methods (sun-, oven- and freeze drying) on antioxidant and antimicrobial activities of date fruit powders from three varieties Zahidi, Aseel and Muzafati were investigated. The DPPH activity was significantly higher in Muzafati sundried date powder, whereas maximum phenolic and flavonoid contents were significantly higher in Muzafati freeze dried date powders. Results suggest that freeze dried date fruit powder had the highest potentia...

Asma Sohail1*, Kashif Sarfraz Abbasi1, Maryum Arif2 and Fatima Najam1 

...ntrapment i.e. extrusion method and indirect diffusion method using Ca-alginate. Pumpkin seed oil macrospheres showed 91.9 % encapsulation efficiency and 63.0 % loading capacity by direct extrusion method. However, indirect or diffusion method showed 43.0 % and 24.7 %. These results showed that direct method of encapsu...

Muhammad Arshad*, Sabeeta Jan, Sundas Awan, Samra Azam, Shiguftah Khalid and Muahmmad Ayub Khan 

...y. Based on WARD’s method genotypes were assigned into seven clusters at 25% linkage distance. Hybrids in Cluster II and IV with high seed yield and oil content were selected as desirable hybrids and suggested to exploit further under diverse environments. 

...
Adem Aksoy1, Yakup Erdal Ertürk2, Ecevit Eyduran3 and Mohammad Masood Tariq4,*
...ddition to least squares method were scrutinized comparatively in the prediction of weight at the end of grazing period. The best statistical approach was selected based on goodness of fit criteria viz. determination coefficient (R2), adjusted coefficient of determination (R2ADJ), and Pearson correlation coefficient between the actual and the predicted values in the response trait handled. The greatest importance order w...
Sajid Ali Khan1, Mazhar Hussain Ranjha1, Azhar Abbas Khan2,*, Muhammad Sagheer1, Amjad Abbas1 and Zeshan Hassan2
...ed using area preference method while for the growth regulatory potential of plant extracts, the adults insects were released in wheat grains treated with different concentrations of plant extracts. All the experiments were carried out in an incubator at 30±2ᵒC and 65±5% R.H using Completely Randomized Design (CRD). The results for repellency remained more significant for C. procera extract as compared to D. alba.While for growth ...

 Nazish Mazhar Ali1, Saiqa Andleeb2, Bushra Mazhar1, Anum Imtiaz1, Shaukat Ali2*

Assessment of antibiogram and resistogram of pathogenic bacteria isolated from irrigated wheat field water
... and agar well diffusion methods. Optimum temperature for growth of all bacterial strains was 37oC and pH 7 except Salmonella which had pH 6. Among all the antibiotics Oxacillin was less potent as three bacterial strains showed resistance against it while others antibiotics were more potent. Minimum inhibitory concentration of heavy metals for all bacterial isolates was different. It was concluded that irrigated pathogenic bacteria are highly resistant to heav...

 Hafiz Muhmmad Tahir1*, Rabia Yaqoob2

Comparing the efficiency of Taq DNA polymerase and PuRe Taq Ready-To-Go PCR beads in amplifying 12S and 16S ribosomal genes
... developed amplification method, PuRe Taq Ready-To-Go PCR beads. One hundred seventy samples, including both fresh and up to five years old tissue samples, were compared. Taq DNA polymerase was found to be less efficient compared to the PuRe Taq Ready-To-Go PCR beads for amplification of the 12S rDNA gene. However, difference in the efficiency of both procedures was not statistically significant for the 16S rDNA gene. Furthermore, Taq DNA polymerase was found ...

 Abbas Ali1, Muhammad Altaf2*, Muhammad Samar Hussain Khan3

Winter survey of birds at Keti Bunder, district Thatha, Pakistan
...sing direct and indirect methods. During the survey, total of 49 winter season bird species belonging to 33 genera
and 21 families were recorded. A total of 4280 birds were recorded dedicated survey effort from the Keti Bunder.
Dominance (D), Shannon-Wiener diversity Index (H’), Simpson Index (S), Evenness (E) and Margalef (R) were
observed form the study area as; 0.06, 3.23, 0.94, 0.52 and 5.74 respectively. During the surveys noted...

 Khurram Shahzad1*, Muhammad Naeem Khan1, Farhat Jabeen2, Nasreen Kosour3, Muhammad Sohail1, Muhammad Khalil Ahmad Khan1, Munir Ahmad1

Bioaccumulation of manufactured titanium dioxide
... is very useful tracking method to determine
the accretion in aquatic organisms.

...

 Raazia Kiran1, Alya Riaz1, Muhammad Irfan1*, Hafiz Abdullah Shakir2

An overview of pre-treatment methods used for bioconversion of lignocellulosic biomasses into valuable products
...br /> The pre-treatment method involves the use of different physical processes i.e., size
reduction, steaming or boiling, ultrasonication, and bursting, chemical methods i.e.,
solvents, acids, salts, bases, the physicochemical processes include liquid hot
water and AFEX (ammonium fibre explosion), biological methods involving whiterot/
brown-rot fungi and bact...

Muhammad Shahzad Akbar, Muhammad Zeeshan Majeed* and Muhammad Afzal 

...dified filter paper disc method according to completely randomized design. All insecticides caused significant mortality of termites as compared to control treatment. Formulations of chlorantraniliprole, chlorfenapyr and pyriproxyfen exhibited maximum termite mortality, ranging from 31.18±5.67, 28.33±7.03 and 21.67±4.01%, respectively at 3 h post-exposure to 100% at 24 h, followed by triflumuron (81.67±3.07%) and indoxacarb (63.33&p...

Abid Muhammad Shah1, Safdar Ali1*, Ijaz Ahmad2, Gulzada Wazir1, Obaidullah Shafique1, Muhammad Amir Hanif1, Bashir Ahmad Khan3 and Sumaira Zareen4 

...nt herbicides and sowing methods on wheat productivity and population dynamics of weeds. Different wheat sowing methods (S) were Broadcasting, Line sowing, Ridge sowing, Bed sowing and herbicides used were Ally Max, Clean wave, Starane M. and Buctril Super 60 Ec. The experiment was laid out in RCBD with split plot arrangement having three replications. Hand weeded (weed free), and weedy check (control) plots were also mainta...

Saman Fatima* and Shazia Iram 

... classical and molecular method was used for identification of pathogens. Under classical approach the infected sections from the skin of kinnow was utilized to grow fungal colonies on generalized media, i.e. potato dextrose agar, from which six fungal colonies were also isolated and studied further. Under molecular approach direct DNA was isolated from the infected skin of sampled fruits by using CTAB method. The pathogens ...

Misbahullah, Shad Khan Khalil, Asim Muhammad*, Afza Tabassum and Shah Fahad  

...e environmental friendly methods are needed to explore possibility of weed control and enhance crop yield. Sorghum extract and their application times on weed dynamics, phenology and leaf tracts of variety Atta Habib was sown at the Research Farm of Agronomy, The University of Agriculture Peshawar, Pakistan throughout 2015-16. Used of sorghum extract with three concentrations (1:3, 1:4, 1:5), weedicide (affinity) with three different levels (1/3, 1/ 2, 2/3 of ...
Yan-Guo Han1, Xiao-Li Peng1, Kai Li1, Yu-He-Tian Zhao1, Xun-Ping Jiang2, Guang-Xin E1, Yong-Ju Zhao1, Jun-Hua Ye3, Li Xu3, Qin-Tao Zhao3 and Yong-Fu Huang1,*
...ve, simple, and low-cost method in enhancing the effect of SS DNA vaccine and the growth of ram lambs.
...
Daniel Zaborski,1,* Muhammad Ali,2 Ecevit Eyduran,3 Wilhelm Grzesiak,1 Mohammad Masood Tariq,2 Ferhat Abbas,2 Abdul Waheed4 and Cem Tirink5
... conclusion, the applied method of reproductive parameters prediction was rather ineffective, indicating that more powerful input variables are required to obtain better prediction results.
...
Abderraouf Chouaib Rebbah1, Mohcen Menaa2,*, Salah Telailia3,Menouar Saheb1 and Mohamed Cherif Maazi2
...ea using the point count method to describe the composition of woodland breeding avifauna and to analyze the spatial distribution among habitat types (oak woodlands, pine woodlands and oak-pine mixed woodlands). A total of 69 species were observed. One species was recorded only in mixed oak-pine forests, six were found exclusively in oak woodlands and 17 species were found only in pine woodlands. We noted 20 protected species, only one endangered species, and ...
Muhammad Furqan1, Usman Ali1,*, Anjum Ara1, Muhammad Mudassar Shahzad2, Muhammad Siddique Awan3, Riaz Aziz Minhas3, Khawaja Basharat Ahmad3, Qamar Zaman Qamar4 and Naeem Iftikhar Dar5
...t analysed using quadrat method. Mongoose was distributed with average population density of 9.8 animal/km2, with highest (17.8 animal/Km2) density at Kalyal and lowest (3.5 animal/km2) at Panyam. Most favored altitudinal range was 450 m to-550 m (asl). Population density was the highest (12 animals/km2) in October and lowest (7.4 animals/km2) in May. Dominant vegetation included Acacia nilotica (IV...

Babar Tasim1, Tariq Masood1*, Zafar Ali Shah1, Muhammad Arif2, Ata Ullah1, Ghazal Miraj3 and Muhammad Samiullah4 

...amples by using standard methods. Statistical analysis of the data revealed significant difference for proximate composition, pH, EC and macro and micro nutrients. Biochar obtained from Sesbania stem was found high in fixed carbon content (66.9%) and volatile matter (27%) but low in ash content (4.5%). AD biochar contained lowest amount of fixed carbon (49%) and volatile matter (17%) but highest ash content (30%) among different biochar samples under investiga...
Aqeel Mehboob, Syed Muhammad Zaka*, Muhammad Sarmadand Maryam Bajwa
... pesticides, alternative methods like botanical extract in the control of this pest consider greater importance nowadays. The antifeedant and oviposition deterrent index of Moringa oleifera, Murraya paniculata and Piper nigrum leaf extracts were evaluated against S. litura by choice and no choice method under laboratory controlled conditions. In M. oleifera extract, the maximum antifeedant ...

Khwaja Tariq Ziad1, Umar Hayat1, Muhammad2 and Muhammad Suleman Bacha3* 

...se study and qualitative method approach, eight key informants including five farmers, one local middleman, and representatives of two dairy companies were interviewed during the study, focusing the social aspects and people behaviour towards the dairy sector. Results showed a lack of coordination among small-scale farmers and associated high transaction costs. This case study suggests that small-scale farmers are illiterate and not properly trained. The way t...
Muhammad Huzaifa Mehmood1, Muhammad Ahmad Iqbal2, Muhammad Daood1, Muhammad Rizwan Tariq3*, Khubaib Ali3
... 8 weeks. Followings the method illustrated in previous studies fatty acid and physico-chemical analysis were performed. The pH texture, fat, protein, blood lipid profile and PUFA concentration was significantly affected by the incorporation of oat in rabbit feed. It was concluded that the supplementation of 2% oat in rabbit feed increase n-3 PUFA in meat while improvement in serum lipid profile was observed by 4% supplementation of oat seeds. It was also obse...
Sumaira Maqsood1,*, Muhammad Afzal2, Muhammad Saleem Haider1, Hafiz Azhar Ali Khan1, Muhammad Ali1, Muhammad Ashfaq1, Muhammad A. Aqueel2 and Muhammad Irfan Ullah2
...using diet incorporation method. Two concentrations of a NPV based formulation (Somestar): 1x107 and 1x108 POB/ml, and three concentrations of a Bt based formulation (Dipel): viz., 0.1, 0.3 and 0.5 μg/g, were applied alone and in different combinations against 2nd and 4th instar larvae of S. litura. The highest mortality of larvae was recorded after 7 days of application when both insect patho...
Peng Song1,2, Wei Feng2, Haiying Shi2,Jinsheng Zhao3, Renmin Liu3 and Wei Xu2,*
...red with the traditional method dissolving oil-soluble dye in DMSO, HP-β-CD was used to monitor the decolorization rate of the sudan I and appropriate molar ratio of HP-β-CD to dye (nC:nS=9:1) effectively improved the decolorization rate from 85.45% to 99.45% after 8 reaction.
...
Samiah Shahid1,Jawaria Shaheen1, Wajeehah Shahid2, M. Waheed Akhtar1,3 and Saima Sadaf1,*
...and comparative delta Cq method were used for selection of suitable reference gene out of the three candidate genes. The validation studies were, thereafter, performed on 112 samples including 87 patients and 25 normal healthy controls. The geNorm algorithm exhibited circulating mir-16-5p with the highest expression stability demonstrated by geNorm M value of 0.418. The comparative delta Cq method showed no significant diffe...

 Saira Zaheer1, Abrar Hussain1*, Aqsa Khalil1, Muhammad Mansha1, Muhammad Lateef2

...pared by cold maceration method. The anthelmintic activity was analyzed by adult motility assay (AMA). Levamisole was used as positive control to compare the efficacy of CEE of both plants. Adult worms were treated with different concentrations i.e., 2, 4, 6 and 8 mg/ml of ethanolic extract and motility of the worms was examined after every two to eight hours. Both A. lebbeck and C. sinensis exhibited 88% and 95% mortality at 6 & 8 mg/...

Naseer Ahmed Abbasi1, Mian Noor Hussain Asghar Ali1*, Bilawal Abbasi2, Shakeel Ahmed Soomro1, Najeeb Ahmed Khan Nangraj1, Jam Ghulam Mustafa Sahto1 and Sajjad Ahmed Morio1 

...parametric and fuzzy set methods in the land assessment for wheat production of Tando Allahyar district of Sindh Province. The present study able to provide grade performance suitability of lands for agricultural purposes; it could be more helpful for farmers of the research area. Fuzzy AHP methods could be beneficial for future studies for land suitability. The study finding have capabilities of different

Muhammad Aslam Rajput1,3, Imtiaz Ahmed Khan2, Rehana Naz Syed3 and Abdul Mubeen Lodhi3* 

...The pathogen inoculation method should be less cumbersome, simple, and rapid. Here, we compared the effectiveness of six different inoculation methods, i.e., dipping, paste, wound+paste, soil infestation, spraying and injection methods for the establishment of smut disease. It appears that disease expression was significantly influenced by pathogen inoculation meth...

Rehmat Ullah* and Khalid Nawab 

...rough personal interview method whereas binary logistic regression model was utilized (binary responses (1 if exists 0 otherwise) both of dependent and independent variables) to find out the effect of precautionary measures and Personal Protection Equipment on self-reported acute poising cases. Binary logistic regression analysis depicted highly significant negative association between adverse effects of pesticides like headache, dizziness, feeling weak, diffi...
Khalid Khan1, Saima Liaqat2*, Somaiya Rasheed1 and Ihsanullah Kakar3
...r, orthodox and dogmatic methods, inadequate awareness and lack of animals’ hospitals lead to hamper livestock efficiency than the potential. Therefore, it is urged that government should make veterinary services available to the farmers at their door steps to improve their income, livelihood and standard of life. Moreover, this can be one of a feasible solution to the problems of poverty, malnourishment and starvation in backward and undeveloped areas l...
Muhammad Hidayat Rasool1,*, Muhammad Zaheer1, Muhammad Farooque Hassan2, Muhammad Shafique1 and Muhammad Usman Qamar1
...standard microbiological methods. Isolated GNRs were subjected to susceptibility testing against various antibiotics as per Clinical and Laboratory Standards Institute (CLSI) guidelines. Carbapenem-resistant isolates were subjected to detection of Metallo-beta-lactamase production by Double Disc Diffusion test (DDDT) and Modified Hodge’s test. In 152 specimens, different species identified in order of their frequency were; Klebsiella pneumoniae (5...
Ayesha Chaudhary1, Fouzia Mumtaz1, Muhammad Yaseen2* and Muhammad Younis Afzal3 
 
...ng a multistage sampling method with the help of well-structured interview schedule. At first, on the basis of simple random sampling Faisalabad saddar tehsil was selected. Six villages were selected from two union councils using simple random sampling. The villages were Dhol Mahjra, Lilan, Sar Shameer, Javadi, Mulanpur, and Suman. In the last stage, 30 respondents from each village were selected randomly. The total sample thus comprised of 180 farmers.&n...
Shahid Yousaf1*, Salman Khurshid1, Saqib Arif1, Hafiza Mehwish Iqbal1, Qurrat ul Ain Akbar1, Najmus Sahar1 and Ghulam Rasul2
...using NIR and referenced methods shown to have average repeatability of 0.2%, 0.3%, 2.4% and 0.1%, 0.7%, 1.5% respectively. The information is useful for maintaining the quality of wheat intended for milling and ultimate products.
...
Osman Ergene1,*, Isfendiyar Darbaz1, Serkan Sayiner2 and Selim Aslan1
...s determined by the TRUS method on the 40th day and the diagnosis of EM were not individually reliable in every animal. However, it is thought that in between 28th day and 40th day, statistically significant difference (P<0.01; P <0.001 between the pregnant and EM animals can be predicted.
...

Sabi-Ur-Rehman1, Anwar Khalid2, Qazi Najam Us Saqib3, Farooq Ahmad1, Shaheed-Ur-Rehman4, Neelam Zaman3, Ayeza Mehmood5 and Abdul Samad6*  

...na, using disc diffusion method. The tested bacterial strains include, B. subtilis, E. coli, S. aureus, P. fluorescens and fungal strains were Aspergillus. niger, A. flavus, and D. turcica. The maximum zones of inhibition, 23.00±0.56 mm and 25.00±0.50 mm was given by ethyl acetate fraction of leaves against P. fluorescens and A. niger respectively. The crude saponins isolated from leaves did not give significant results against the tested bacteri...
Hande Küçükönder1, Pınar Çelebi Demirarslan1,*, Aykut Burgut2 and Mustafa Boğa3
...hs are determined by DEA method and a performance rank is performed between the effective months by GRA method. It has been investigated whether the results of different Multicriteria Decision Making (MCDM) techniques combined with the data fusion technique support the proposed hybrid approach’s results. In this context, the proposed hybrid approach was applied to evaluate the monthly production performance of a commer...
Umar Farooq Gohar1, Hamid Mukhtar1,*, Ali Nawaz1, Ikram-ul-Haq1 and Asad Mehmmood2
...sessed by disc diffusion method. Cephalosporin production was estimated by using spectrophotometric technique.
...
Doğukan Ölmez1 and Gonca Alak2,*
...re prepared by enzymatic methods at different time periods using different trout byproducts. In this study, two different groups (viscera and muscles) of trout processing wastes were hydrolyzed by enzymatic method based on specific time periods (0, 30, 60, 90, 120 and 180 min) for this purpose. Protein profiles, molecular size and distribution changes of the obtained hydrolysates were calculated using SDS-PAGE and lipid prof...
Saleh S. Alhewairini1,* and Mahmoud M. Al-Azzazy1,2
..., an alternative control method was developed for camel tick (Hyalomma dromedarii Koch) and cattle tick (Hyalomma impeltatum Schulze & Schlottke), to replace the widely used traditional acaricides. Huwa-San TR50 is a formulation of hydrogen peroxide, stabilized by the addition of a small quantity of silver and is extensively used as a disinfectant. Both tick species were susceptible to certain concentrations of Huwa-San TR50: 4,000, 8,000, 10...

Ayesha Khan*, Zubair Ahmad Khan, Urooba Pervaiz And Mehmood Iqbal 

...raints and the extension methods used by them. All agricultural officers and 40% of field assistants were interviewed making a sample of 81 extension agents; 10 agricultural officers and 71 field assistants. The study results indicate that 77% field assistants were educated up to Matric with two years diploma while 10% agricultural officers had M.Sc. (Hons) degree with the majority of extension agents having job experience of more than 20 years. The main sourc...
Tahira Moeen Khan* and Amat ul Mateen
...ensive chemical/physical methods. A cheaper, simple to perform method giving high yield without by products is employed here. The leaf extract act both as reducing and capping agent. The atomic absorption shows a linear curve between concentration and absorbance. The uv-vis spectrum revealed the shift of absorption towards longer wavelength with the increasing concentration of plant extract. XRD indicates CuO Nps particle si...
Jason Shepard1 and Tyra Turner2
...e studies using big data methods to test whether the relation between future-oriented thought and well-being generalizes to non-WEIRD cultures. We found evidence that the relation between future-oriented thought and well-being was robust across cultures, including when analyzing a general trend across 64 countries (Study 1) and when examining specific cultural contrasts (Study 2 and Study 3). Taken together these three studies provide evidence that the relatio...

 Zhi Yijin and Shao Mingqin*

...> The line transect method was used to examine the population size and sex ratio of the scaly-sided merganser Mergus squamatus (the merganser) in four rivers of the Poyang Lake water system, Jiangxi province, China. Spatial and temporal variations in population size were ascertained for this endangered species. The Xiuhe, Fuhe and Raohe river systems exhibited large steady populations, with the maximum size ranging from 25 to 68 individuals for eac...

Farhana Gul1*, Dawood Jan1 and Muhammad Ashfaq2 

...d fertilizer application method and increasing sowing densities were three-major adaptation practices whose benefits were analyzed. The adaptation results indicated that adopters of adaptation technology would range from 33 % to 74.47 % in Dera Ismail Khan and 62 % to 65.18 % in Peshawar. Before adaptation, poverty rates would range from 23 to 28.90 percent in D.I.Khan while from 19 to 28.62 percent in Peshawar for all GCMs. These findings concluded that prop...

 Jawaria Shaheen1, Samiah Shahid1, Sabahat Shahzadi2, M. Waheed Akhtar1,3 and Saima Sadaf1,*

...>-ΔΔCt method. Further analysis was done by SPSS, medcalc and Graphpad prism software. Area under curve analysis was used to get diagnosis outcome of selected miRNA panel. Upregulation of miRNA panel (mir-376c, mir-155, mir-17a and mir-10b) has been seen in the plasma samples of TNBC patient´s samples relative to non-TNBC control samples. The scatter plots and other statistical analysis have given differences in expression of four miRNA...

 Abdul Maalik1, Shahzad Ali1,*, Anam Iftikhar1, Muhammad Rizwan1, Haroon Ahmad2 and Iahtasham Khan1

...s analyzed by chi-square methods at 95% confidence interval. The overall prevalence of subclinical mastitis as a result of surf field mastitis test was 42.2%. The prevalence of disease was higher in cows (54.5%) than in buffalo (22.9%). Based on chi-square and P-value the prevalence of mastitis was significantly (P˂0.05) associated with animal type, urbanicity, age of animal and previous exposure of mastitis. There was no association of the prevalence of bovi...
Faiz-ul Hassan1,*, Muhammad Sajjad Khan1, Muhammad Saif-ur-Rehman1, Syed Muhammad Raihan Dilshad2 and Muhammad Amjad Ali3
...was constructed by UPGMA method to determine the genetic similarity among the horse breeds. Thoroughbred and Arab horse breeds showed a higher similarity with Pakistani indigenous breeds, suggesting that these two exotic breeds had contributed significantly in the development of indigenous horse breeds of Pakistan. One set of primers OPL-7 revealed a polymorphic band at 450 bp which was present in DNA bulk of the indigenous breeds while absent in that of exoti...
Sumreen Hayat1,6, Muhammad Hussnain Siddique2, Bilal Aslam1, Habibullah Nadeem2, Asma Ashraf3, Muhammad Saqalein1, Mohsin Khurshid4, Naveed Shahzad5 and Saima Muzammil1,*
...rmined by disc diffusion method and isolates were screened for ESBL production using double-disc synergy test (DDST). Molecular detection for the presence of bla-SHV and bla-TEM was carried out by polymerase chain reaction (PCR). Of 392 blood and urine samples, 120 were found positive for microbial growth. Antibiotic susceptibility testing of K. pneumoniae isolates illustrated high resistance to ceftazidime (95.6%). Phenotypically,52.17% o...

Muhammad Adnan Islam1,2*, Muhammad Iqbal2, Zia-ul-Haq2,3, Muhammad Mohsin Ali1, Hafiz Sultan Mahmood1, Shabbir Ahmed Kalwar1, Liaqat Ali Shahid1, Badar Munir Khan Niazi1 and Muzammil Husain1 

...paration by conventional method. Zone disc tiller machine developed in the department of Farm Machinery and Power, was employed for sowing wheat instead of conventional tillage in cotton stubble to avoid further late sowing of wheat. Zone disc tiller drill sows crop in strips. Strip tillage of soil reduced labour, fuel, irrigation and machinery costs. Zone disk tiller was tested for its field performance with three different types of furrow openers viz; disk f...
Zahra Nazir1, Saba Ijaz1, Roquyya Gul2 and Mahjabeen Saleem1,*
...ased on surface response methodology were developed that showed significant impact of independent variables like enzyme concentration, temperature and incubation time on the dependent variables and improved the overall quality of apple juice. Hence, acidic plant polygalacturonase isolated in this study would be useful as a potential candidate for its biotechnological applications in fruit juice clarification.
...

Hafiz Umar Farid1, Muhammad Zubair2, Zahid Mahmood Khan1, Aamir Shakoor1*, Behzad Mustafa1, Aftab Ahmad Khan3, Muhamad Naveed Anjum4, Ijaz Ahmad5 and Muhammad Mubeen

... conventional irrigation methods. However, the most burning issue of these systems is their non-adoptability or low adoptability by the farming communities, despite their proven benefits. It was highly desirable to analyze and evaluate certain parameters which keep systems functional as well as ensure adoptability of these systems at farmer’s fields. A total of 30 sites were selected randomly in Punjab province of Pakistan where HEIS were installed. Twel...
Rıfat Ertekin1*, Mete Ozkurt1, Gokhan Kus2 and Selda Kabadere1
...uire that new, effective methods or chemicals should be developed. Tolfenamic acid (TA) is a nonsteroidal anti-inflammatory medicine stimulating apoptosis in cancer cells like pancreas, oesophagus and lungs. This study aims to determine the role of TA in cell viability and apoptosis in glioma cells (T-98G) and androgen-sensitive human prostate cancer cell line (LNCaP). In our study, 1, 5, 10, 25, 50, and 100 μM doses of TA were applied to T-98G and LNCaP ce...
Farhat Iqbal1, Abdul Waheed2,*, Zil-e-Huma3 and Asim Faraz2
...ast squares and Bayesian methods. Using the monthly body weight data of 2070 weight records of Thalli sheep from birth to 24 months of age for both sexes (male and female) and type of births(single and twin), the estimates of parameters and their credible intervals were obtained from posterior distributions. The overall goodness of fit of classical method was calculated; namely, Akaike information criterion (AIC), Bayesian i...
Emel Banu Buyukunal1,*, Rojan Ibrahim Albazaz1 and Mehmet Ali Bal2,3
...nventional and molecular methods for E. coli isolation in this study. Subsequently, antimicrobial susceptibility profiles of E. coli isolates (n=71) were determined against thirteen agents; amoxicillin-clavulanic acid, piperacillin-tazobactam, ceftazidime, cefotaxime, ceftriaxone, cefpodoxime, aztreonam, ciprofloxacin, amikacin, ertapenem, imipenem, meropenem, trimethoprim-sulfamethoxazole by disk diffusion method<...

Muhammad Usman Ghani1*, Muti Ur Rehaman Khan1, Asim Aslam1, Zubair Shabbir2, Li Bo3 and Naveed Anwar4 

...lar diagnostic tool. The method relies on periodically thermo cycling heating and cooling of DNA in presence of certain chemical reagents. In order to detect and differentiate parapox infections from those induced by pox virus. A new parapox specific and sensitive PCR assay based on amplification of B2L gene was applied against conserved gene of Orf virus. Crusted scraps and biopsies from different portion of skin and blood were taken as sample for PCR. Gross,...
Ailing Huang, Lingyu Meng, Wei Zhang, Junyan Liu, Guangyao Li, Huihua Tan, Wen Lu and Xialin Zheng*
...d using the leaf-dipping method. Meanwhile, effects of these pesticides on detoxifying and protective enzymes in P. flammans larvae were also measured. Results showed that the LC50 value of beta-cypermethrin, abamectin, chlorpyrifos, thiamethoxam and bisultap against P. flammans larvae were related to the instar and pesticide. Control efficacy of beta-cypermethrin was the best among of these pesticides, and the LC50 value of...
Sun Hong1, Zhang Yifeng1, Wang Baishi2, Li Yangwei1, Xu Wenbo1, Mao Runkun1 and Wang Zhenglong1,*
...bedded artificial tunnel method in a natural burrow system to measure oxygen (O2) and carbon dioxide (CO2) concentrations and the temperature and humidity in the underground burrows of mandarin voles in Xinzheng, Henan Province, China during the spring and summer of 2015. Our results show that 1) the depth and complexity of the burrow were higher during summer than during spring; 2) maximal CO2 levels (2.55%) and minimal O...

Ayat Ullah* and Ayesha Khan 

...cal and cultural control methods in the rain-fed districts of Khyber Pakhtunkhwa, Pakistan. Using multistage random sampling technique data were collected from 395 respondents according to Yamane’s formula. The study uses the Kruskal–Wallis H test for checking differences among farmers knowledge with differences in their contact with extension service providers. The results revealed significant differences in farmers’ knowledge of chemical, b...

Abdur Rehman1*, Iqbal Javed2, Zhang Nannan3, Muhammad Niamatullah1, Raheel Saqib4 and Allah Bakhsh

...pted the system analysis method to focus on the existing problems and to put forward the significant and the specific guidelines. The results showed that the Lang fang city is located between Beijing and Tianjin stands first in the comprehensive level of ranking for the development of agricultural informatisation and remained first in the ranked of index popularisation and application of agricultural informatisation. The Tangshan city ranked first for the infr...
Sher Bahadar Khan1,2,*, Zou Geng1, Cheng Yu-ting1, Asad Sultan3, Mumtaz Ali4,5, Irshad Ahmad6 and Rui Zhou1
...ng to the disc diffusion method and antibiotic resistant genes {tet(A), tet(B), tet(C), strA/strB, aadA, aac(3)IV), aadB, sul1, sul2 and sul3, blaCMY-2, blaTEM and blaSHV} using mPCR. Resistance for LIN was the highest in overall (96.3%, 77/80) isolates as well as from pig farms (100%, 20/20) and different markets (100%, 20/20, 85%, 17/20). Resistance for other antibiotics such as AMX, TET, AMP and SXT was found 82.5% (66/80), 63.7% (51/80), 58.7% (47/80) and ...
Ghulam Murtaza1*, Razia Sultana2, Muhammad Arshad Malik3, Sibtain Afzal4, Mubashar Hussain5, Shahid Bashir6
...g habit using the latest method such as transcranial direct current stimulation.
...
Pin Lyu1, Xiangxian Chen2* andQinlong Liu3*
...esponding rehabilitation methods. The injured muscular tissues and blood samples were collected pre-injury and post-injury on day 1, 2, 4, 6, 9, 12 and 15. The exercise and massage group showed the fastest recovery reflected by muscular histological structure and C-reactive protein levels, followed by the other three groups. C-reactive protein decrease in exercise and massage, massage only or exercise only groups was significantly different comparing to contro...
Bo Zhang1, Changbo Zhu1, Zihao Meng1,4, Baosuo Liu1, Lian Zhong3, Guiju Huang1, Jiaqi Su1, Sigang Fan1 and Dahui Yu1,2,*
...tablish a more efficient method to assess carotenoid contents, we measured the carotenoid levels in selectively bred P. fucata individuals of different colors and analyzed the correlations between TCC (total carotenoid content) and color parameters. The percentage of total carotenoids in the adductor, gill, mantle, and visceral mass was 6.50%, 10.79%, 15.11% and 67.61%, respectively. Generally, the tissue-specific carotenoid distribution is ranked in th...

Waqar Akhtar1*, Munir Ahmad2, Nadeem Akmal1, Hassnain Shah1 and Asif Ali Mirani2 

...cy Analysis Matrix (PAM) methodology. Relevant indicators were employed to gauge and compare resource use efficiency of two dates products (fresh dates and dried dates) produced by farmers. The finding of positive Private Profitability and Private Cost Ratio (PCR) value less than 1 argues farm level competitiveness under small, medium and large farms in the study area. Fresh Dates has more competitiveness with less PCR value 0.62 compared to dry dates with PCR...
Zhengfei Wang*, Dan Tang, Xuejia Shi, Huayun Guo, Xiuping Chen, Daizheng Zhang and Boping Tang*
...nt crabs by at least two methods. A series of putatively selected codons was localized in or close to the important functional regions (protein binding region and helical transmembrane region) in the mitochondrial protein structure.These results help explain why Bythograeidae crabs are capable of living in the hydrothermal vents and suggest that these crabs might have acquired an enhanced capacity for energy metabolism in an extreme hypoxic environment. These ...

Hafiz Abdul Ghafoor*, Muhammad Afzal, Muhammad Luqman and Muhammad Zeeshan Majeed 

...andard twig-dip bioassay method according to Completely Randomized Design. Results showed that both factors i.e. insecticidal treatments (F 9, 245 = 146.90, P < 0.001) and time (F 4, 245 = 445.75, P < 0.01) and their interaction (F 36, 245 = 9.20, P < 0.001) had a significant effect on the mortality of mealybug nymphs. The most effective novel chemistry insecticides against D. mangiferae mealybugs were sulfoxaflor, spirotetramat, thiamethoxam and pyri...
Xiaohui Yu, Shuai He, Liqiang Wang, Mengyang Kang, Yanjiao Zhu, Shuhui Wang and Xiuzhu Sun*
...tion offers an effective method to protect Guanzhong donkey, a precious species. The objective of this study was to reveal the effect of different antioxidants on the semen cryopreservation in Guangzhong donkey by testing indices of sperm motility, mitochondrial activity, membrane integrity and arosome integrity. The results illustrated that compared with controls, above-mentioned indices of sperm were significantly improved under concentrations of Vitamin C (...
Iram Liaqat1,*, Nazish Mazhar Ali2, Najma Arshad3, Riffat Iqbal1 and Zain-ul-Abideen1
... p<0.05) by test tube method compared to liquid interface coverslip assay (35%) and congo red assay. D. vulgaris was treated with various concentrations (5, 40, 80 and 100µM) of salicylidene acylhydrazide and 5-15 µM of proteases (proteinase K, trypsin, and chymotrypsin). Concentration dependent decrease was observed after 72 hours in biofilm formation of D. vulgaris on treatment with salicylidene acylhydrazide. Whereas 15 µM...

Tahira Jatt1, Ghulam Sarwar Markhand1, Lane Rayburn2, Ray Ming3, Mushtaque Ahmed Jatoi1 and Ameer Ahmed Mirbahar1,4* 

...aditional Fuelgen squash method. Results indicated that all seven commercial varieties and four wild types of date palm are diploid in nature and having 2n=36 chromosomes. First time detailed karyotype of these varieties have been developed which includes total chromosome length (TCL), relative length (RL) and centromeric index (CI). The chromosome complement consists of metacentric (m), sub-metacentric (sm), sub-telocentric (st) and telocentric (t) chromosome...
Wafaa M. Hikal1,2,* and Hussein A.H. Said-Al Ahl3
...sistance to disinfection methods. The aim of this study was to isolate the pathogen from the dental units and hospital water network and treatment of Acanthamoeba with Trachyspermum ammi essential oil that have the ability to kill cysts. The amoebicidal activity of T. ammi oil was determined in vitro. Out of T. ammi oil showed that, higher concentrations (90 μg/mL after 72 and 96 hrs and 100 μg/mL after 24 and 48...
Hafiz Abdul Ghafoor*, Muhammad Afzal, Muhammad Asam Riaz and Muhammad Zeeshan Majeed
...duals. Standard twig-dip method was used for toxicity bioassays according to Completely Randomized Design. Mortality of mealybug individuals varied with plant materials and increased along with the extract concentration and exposure time. Botanical extracts of Azadirachta indica (neem) and Gardenia jasminoides (gardenia) were the most effective with minimum LC50 (20.00 and 42.19%, respectively) and LT50 (47.97 and 71.26 h, r...
Farah Bilal*, Abdulmohsen Alhejaily, Shahida Husnain
...cing with sangers’ method. Only one sample detected p.E545K mutation at c.1633G>A. Another most common mutation was observed in PIK3CA exon 20 which leads to change A>G at codon 1047. This transition converts amino acid histidine to arginine. Our study concluded p.H1047R most frequent while p.E545K rarely found mutations in PIK3CA gene in NSCLC population. Present study can be proved as road map in setting PIK3CA mutations as p...
Xuefeng Cao1, Guangneng Peng1, Xiaobin Gu1, Changliang He1, Guizhou Yue2, Jun Shi3,* and Zhijun Zhong1,*
...aqMan real-time (RT)-PCR method (DARPM) to detect CPV and CDV in clinical samples. We compared this new method against real-time PCR/(RT)-PCR and it showed no cross-reactivity with other pathogens. Sensitivity testing showed the minimum detection limits of real-time (RT)-PCR were 7.44×101 copies·μL-1 (CPV) and 4.20×101 copies·μL-1 (CDV). DARPM detect...
Jingchen Chen, Zhaochao Deng and Zhiqiang Han*
...gh-throughput sequencing method is the methodology of choice for generating complete mtDNA genome sequences.
...

Yasir Ali1,2*, Bashir Ahmad1, Naqeebullah Jogezai3 and Adil Hussain

...n agar (TBA plate) assay method. From the collected samples, a total of 20 bacterial isolates (PAK-01 to PAK-20) were exhibiting psychrotrophic physiology. About 8 isolates were capable of proteolysis in alkaline range of pH; and 6 bacteria exhibited lipolysis. Based upon primary and secondary qualitative screening, One isolate (PAK-01) exhibited maximum proteolysis on 1 % skim milk while another isolate (PAK-03) demonstrated maximum lipolysis on Tributyrin Pl...

Ayub Khan* and Ghaffar Ali 

..., a contingent valuation method (CVM) was used to elicit households WTP. A sample of 109 households was randomly selected from 14 randomly selected villages two from each tehsil. The study results revealed that most of the respondents perceived the problem of soil erosion and their willingness to pay for the suggested forestation based soil conservation practices were on average Rs.4666 per year. Regression analysis revealed that slope of the land, family size...
Jae-Kang Lee, Hyun-Su Hwang, Tae-Kyung Eom and Shin-Jae Rhim*
...used a capture-recapture method to investigate the ecological factor influencing small rodents in a tree thinned Japanese larch Larix kaempferi plantation in Mt. Maehwa, Hongcheon, South Korea. The mid-story, understory and ground vegetation coverage, the number of standing and downed trees ha-1 and the volume of downed trees ha-1 were significantly different between the pre- and post-thinning sessions. Mean density of Apodemus ...
Ali Mujtab Shah1,2,3, Muhammad Naeem2, Muhammad Giasuddin Shah2, Muhammad Haaroon2, Quanhui Peng1 and Zhisheng Wang1,*
...arious colostrum feeding methods on growth performance, and immunity of Holstein Friesian (HF) calves. The applied feeding methods were stomach tube (ST), nipple feeder (NF) and natural suckling (NS). A total of 15 newly born HF calves were selected randomly from the available stock and divided into 3 groups, each of 5 calves. The median birth weight (kg) ± S.D of calves fed by ST, NP and NS were recorded as 28...
Xiaopeng Tang1, Wen-qin Su1 and Re-jun Fang1,2,*
...as determined by the MTT method. Cell morphology and phosphorus concentration in the cell supernatant were measured after 24 h of CT treatment. The NaPi-IIb protein expression was determined by Western Blot, and the NaPi-IIb mRNA expression was determined by RT-PCR. The results showed that, compared with the control group, different levels of CT had no effect on cell proliferation, but it inhibited (P < 0.05) the absorption of phosphorus at CT...
Qihang Liu1,2, Yueli Jiang1, Jin Miao1, Zhongjun Gong1, Tong Li1, Yun Duan1 and Yuqing Wu1*
...rol locusts, alternative methods such as photo-induced traps are elusive. In this report we investigated locusts visual sensitivity to different spectral lights after orange light stimulation. Results showed that after 10, 20, and 40 min of 1000 lx post-orange light stimulation, locusts visual response intensity was stimulated by green, violet, and blue lights. However, post-64600 lx orange light stimulation for 30, 20, and 10 min, the response intensity was t...
Qiuxia Lei1, 2, Haixia Han1, 2, Jinbo Gao1, 2, Yan Zhou1, 2, Wei Liu1, 2, Chunlei Wang3, Jie Liu1, 2, Dingguo Cao1, 2, Huimin Li1 , Baohua Huang1,* and Fuwei Li1, 2, *  
...To develop the effective methods for the innocuous treatment of the poultry manure, this study explored the efficiency of bacteria in decomposing the poultry manure through testing the variation of gas production and nitrogen(N), phosphorus (P), potassium(K)in the fermentation process. Poultry litter, sawdust and rice husk were mixed in equal quantities by volume. One was treated with a single strain of Bacillus; another was treated with a consortium of...

Sanaullah and Urooba Pervaiz* 

...rough personal interview method. Result showed that 63.9% of the respondents were illiterate, 31.3% respondents were in age group between 25-35 years. Yield, income, plant spacing, seed rate, irrigation interval, and use of pesticide before and after training were computed with the help of paired sample t-test. Results revealed that in maize there is a significant decrease for seed rate (-11.6743 kg/acre) and irrigation interval (-16.03 days) after training, w...

Muhammad Waqas, Shahid Ali*, Syed Attaullah Shah and Ghaffar Ali 

...d land and use different methods to make it arable for wheat production. Government also needs to make large reservoirs in unirrigated areas in order to store rainfall water for irrigation of unirrigated land for higher wheat production.

...

Muhammad Arshad1, Muhammad Irfan Ullah1*, Muhammad Afzal1, Mian Anjum Murtaza2, Ejaz Ahraf3, Zahoor Hussain4, Syed Muhammad Ali Zahid1 and Maryam Riaz1 

...ed by the image analysis method using Sigma Scan Pro 5.0 software. The results of the present study showed that CLM generated larger mines; 1.64 cm2 on Grapefruit, 1.44 cm2 on Kinnow and 1.40 cm2 on Succari compared to other five cultivars. However, the percent leaf damage due to the feeding of CLM larvae was observed higher 44.2% on Fairchild, 36.5% on seedless Kinnow, 36.3% on Feutrell’s early and 35.8% on Kinnow that showed great susceptibility to CLM...

Syed Muhammad Hassan Raza1*, Syed Amer Mahmood1, Syeeda Areeba Gillani1, Syed Shehzad Hassan1, Muneeb Aamir1, Muhammad Saifullah1, Mubashar Basheer1, Atif Ahmad1, Saif-ul-Rehman2 and Tariq Ali1 

... tools, datasets and the methodology is easy to understand and apply throughout the world to estimate the net productions precisely. 

...
Abdul Waheed1,2, Sidrah Saleem1, Naveed Shahzad3, Junaid Akhtar4, Muhammad Saeed5, Iqra Jameel1, Farhan Rasheed6 and Shah Jahan7,*
...ST) and combination disc method as per Clinical Laboratory Standard Institute (CLSI) guidelines 2015. Presence of “blaSHV” and “blaOXA” genes was tested by PCR technique. Every isolate was ESBL positive and resistant against third generation cephalosporin and Aztreonam. Notably, 15% of ESBL producing strains harbored blaSHV gene while 43% were positive for blaOXA ge...
Lin Qi, Lu Li, Danyang Chen, Mingxiao Liu, Yan Wu and Wei Hu*
...tudied by disc diffusion method, and the antibacterial ring diameter of each antibacterial agent was measured to determine its antibacterial ability. The molecular mass and purity of this polypeptide was 18.970kDa and 90%. Amino acid sequence analyses indicated that the N-terminal amino-acid sequence of this monomeric peptide was AAQSLLACGAARLYKIIQQWPHYRR. The results showed that under the effect of various bacteriostatic agents, Enterobacter aeruginosa...
Shanshan Cai1, Tianxiang Gao2, Binlun Yan3, Aiyi Zhu1 and Xiumei Zhang1,2,*
...very, yet lack of proper methods hinders the precision and accuracy of such assessment. The swimming crab, Portunus trituberculatus is a commercially important species in Chinese fishery industry. However the natural resources of swimming crabs are declining and enhancement programs are being conducted for resources restoration for decades. In this study, 524 female broodstock from 10 hatcheries and 547 recaptured crabs from 6 investigations were used t...
Sabbah M. Allam, T.M. El-Bedawy, M.H. Bakr and A.E.M. Mahmoud*
...using Acid Insoluble Ash method. Feces samples were taken and analyzed in the same day for Salmonella spp. and E. coli and blood samples for analysis were taken from the subcutaneous abdominal vein. The experimental rations were almost identical in chemical composition. Results of nutrient digestibility and nutritive value, as total digestible nutrients (TDN) and digestible crude protein, showed that there were insignificant (P>0.05) diff...
Mahdi Khojastekey1, Majid Kalantar1, Jam Zaheer Ahmed2, Samiullah Khan3, Muhammad Altaf Hussain4, Rajwali Khan5 and Sayed Haidar Abbas Raza6*
...s of accelerated lambing method overall profits of the herd, based on the prices in the market in years 2013 and 2019, increased 18.36 and 45.52 dollars/ewe, respectively. The results of this study showed that zandi ewes have a good function in accelerated lambing system due to their specific physiological characteristics. According to the current conditions of Iranian economy, using this method to increase livestock profit ...
Muhammad Zameer Khan1, Sair Sarwar1*, Ahmad Khan1, Razaullah Khan1, Munazza Yousra1 and Muhammad Ilyas2
...d that AB-DTPA soil test method can safely be used for evaluation of K status of soils derived from diverse parent material.
...

 Li-na Li1, Sheng Li2, Ping Gui3, Jing-hui Li1, Fen Ai4,* and Li-li Cai1,*

...No effective therapeutic method is available for the treatment of radiation pulmonary fibrosis. Recent studies have suggested that urine can provide an effective and noninvasive source of many differentiating or differentiated progenitor cells and stem cells. The aim of our present study was to assess the therapeutic effects of human urine-derived mesenchymal stromal cells (uMSCs) in a cell model of radiation pulmonary fibrosis. MRC-5 cells were irradiated and...
Muhammad Mansoor1, Khalid Usman2*, Shahid Hameed Khan Khalil3, Abdul Mateen Khattak4, Muhammad Ehsan Elahi1, Muhammad Waqas Imam Malik2 and Amir Hamza2
...p;
...
Himanshu Mishra, Vikas Kumar and Ashish Kumar*
...ation. The line transect method was employed for population estimates. During the field survey, we recorded a significantly higher number of migratory birds at the end of early winter (December) and at the commencement of middle winter (January). Red crested pochard (Netta rufina), Common coot (Fulica atra) and Gadwall (Mareca strepera) were the most populated species in the present study. The number of birds was not significantly differen...
Zhijun Zhong1, Rui Tu1, Xichun Wang2, Yi Geng1, Qicheng Xiao1, Yinan Tian1, Bin Wei1, Jiaming Dan1, Ya Wang1 and Guangneng Peng1,*
...n in pet dogs. Molecular methods, combining three types of PCR assays, identified two strains isolated from two pet dogs as Brucella canis. Histopathological changes revealed extensive inflammation and necrosis in the liver, lung, spleen, kidney, testicle, and lymph nodes, among which changes in the spleen were the most serious. Immunohistochemistry results demonstrated the detection of B. canis antigens in the lesions of all examined tissues. St...
Qi Ren, Shufeng Sun, Chen Niu, Yuhong Li, Yingzhi Chong, Biao Li, Guoying Zheng and Fumin Feng*

 

...ation-specific PCR (MSP) methods. The increases in the methylation rate of CpG islands in the CYP1A1 promoter region and expression of DNA methyltransferases (DNMTs). And then the CYP1A1 mRNA and protein expression decreased with cell damage, thus suggesting that elevated methylation of the promoter region of the CYP1A1 gene may affect its expression and could be associated with cell damage. After treatment of the cells with AZA, increases in methylation rate ...
Muhammad Siddique1, Muhammad Shafique2,*,Abdul Mateen3, Naveed Shahzad4, Bilal Aslam2, Nasir Ahmad1 and Muhammad Usman Qamar2
...ti-HCV antibodies by ICT method. Among positive samples, confirmation was carried out by RT-PCR and clustered into four groups (acute, chronic, cirrhotic and hepatocellular carcinoma) on the basis of clinical reports and the patient’s history. HCV genotypes were determined by RFLP using amplified products and modified multiplex RT-PCR. Out of 3200 clinical samples, 5.37% (n=172) samples were HCV positive by ICT method....
Hande Kucukonder1, Pinar Celebi Demirarslan1*, Sezai Alkan2 and Özgür Baris Bırgul3
...y Model (1,1) prediction method were used. The time-dependent growths of mixed sexes broilers were analyzed in the aspects of testing the parallelism of female and male growth samples, determining the best fitted growth model and designating the biological meaningful parameters (inflection point age, weight and growth rate) of growth functions. Analyses showed that the growth profiles of female and male chicks found not to be parallel using profile analysis, a...
Shou-Hong Zhang1*, Ying Zhang1, Kun-Ming Wen2, Wei Wu3 and Wen-Yan Li3
...WI might be an effective method for quantifying the severity of liver fibrosis.
...
NaseemZahra1,2,*, Nadia Jamil1, Sajid Rashid Ahmad1, Imran Kalim2, MuhammadKhalid Saeed2, Ijaz Ahmed2, Qurat-ul-ain Syed2 and Soniya Munir1
...ng competitive ELISA kit method. Branded samples (n=40) were collected from different shops in Lahore city of Punjab Province while non-branded samples (n=40) collected from four selected towns of Lahore. Overall 58% and 100% of aflatoxin contamination in branded and non-branded milk samples were found, respectively. The quantities of AFM1 in 58% of branded milk samples and 95% of non-branded milk samples were found beyond permissible limits of 50 ppt as set b...

Illahi Bux Bhatti1, 2*, Imran Khatri2, Maqsood Anwar Rustamani2 and Riffat Sultana

...ion of different control methods i.e. cultural, biological, yellow sticky traps and chemical against sugarcane whitefly during February 2016 at PARC-NSTHRI, Thatta, experimental field. The result revealed that lowest number of whitefly (0.38 nymphs + puparia per cm2 per leaf) with highest % mortality (79.89) was recorded in T11 (cultural control + biological control + chemical control), followed by T10 (Cultural control + Biological control + Yellow sticky tra...

Muhammad Khuram Razzaq1,2*, Saeed Rauf1, Mohsin Khurshid3, Shahid Iqbal1,4, Javaid Akhter Bhat2, Ayaz Farzand5, Adeel Riaz1,6, Guangnan Xing2 and Junyi Gai

 

...viability, selection and methods for the improved pollen viability and the management of heat stress.  

...

Muhammad Shahzad Akbar*, Maria Aslam, Muhammad Rehan Khalid, Shahid Iqbal, Muhammad Luqman and Muhammad Zeeshan Majeed 

... using filter paper disc method according to completely randomized design with four replications for each treatment. Termite mortality data was recorded at 6, 12, 24 and 48 h post-treatment. Median lethal concentration (LC50) and median lethal time (LT50) values were calculated for each treatment using log-dose probit analysis. Results revealed a mortality response of termites directly proportional to the extract concentration for all treatments. The floral ex...

Hala Rajab1, Muhammad Sayyar Khan1*, Safdar Hussain Shah1 and Syed Mehar Ali Shah2 

...the result of floral-dip method, the tissue culture based method led to successful regeneration of shoots on Murashige and Skoog (MS) media containing BAP 3 mgL-1 and developed roots in ½ MS media supplemented with IBA 3 mgL-1 in case of OSCAR cultivar. Successful selection of transgenic lines was achieved using selective antibiotic kanamycin 50 mgL-1. NtSAT4 gene integration into the plants genome was confirmed by P...

Yousaf Noor*, Zahir Shah and Muhammad Tariq 

...ct of Zn and B and their methods of application on the yield and yield attributes of citrus (Citrus Sinensis) orchards established on calcareous soils at three farmers fields in Dubandai and Palai area of District Malakand of Khyber Pakhtunkhwa, Pakistan during 2009-2010. A randomized complete block experiment with split plot arrangement was used. Methods of application (Soil and Foliar) were assigned to main plots while mic...

Umar Hayat1*, Shahid Ali1, Abdul Mateen2 and Hazrat Bilal

...inary least square (OLS) method is used to estimate the model. Empirical results of the study reveal that agricultural growth and other important variables of the study such as cash crops, livestock, fisheries and forestry are significantly affecting poverty head count rate in Pakistan. Thus, long-run agriculture growth programme in line with overall economic development targets of the country needs to be planned on operational grounds with strict implementati...

Shwe Yee Win1, Lat Lat Htun1, Myint Myint Hmoon1, Hla Myet Chel1, Yu Nandi Thaw1, Nyein Chan Soe1, Htet Lin Oo3 and Saw Bawm2* 

...tation and sedimentation methods were carried out to detect the gastrointestinal parasites. The overall occurrence of gastrointestinal parasites in village chickens was 60.5% (121/200). Six species of gastrointestinal parasites were observed as Capillaria spp. (30.0%, 60/200), Ascaridia galli (24%, 48/200), Eimeria spp. (20.5%, 41/200), Raillietina spp. (17%, 34/200), Strongyloides spp. (2.5%, 5/200) and Subulura brumpti (2.0%, 4/200). Mixed infection rate was...
Muhammad Ramzan1*, Muhammad Nadeem1, Masood Maqbool1, Ghulam Murtaza2
...99 were sown on dibbling method during 2017. The present study resulted that no variety was free from the attack of thrips population. Among all tested varieties, NIAB-78 and MNH-886 were found most resistant and susceptible against thrips, respectively. The mean population of thrips on MNH-886 and NIAB-78 were recorded 5.43 and 3.49 per plant, respectively. The study revealed that NIAB-98 (4.92/plant) and NIAB-999 (4.92/plant) were found more attractive varie...
Sobia Nazir1, Muhammad Irfan1*, Muhammad Nadeem2, Hafiz Abdullah Shakir3*, Muhammad Khan3, Shaukat Ali4, Quratulain Syed2
...sign of response surface methodology. Maximum activity of CMCase (0.700 IU/gds/min) and FPase (0.260 IU/gds/min) was observed on 4th day with inoculum size of 1% and medium volume of 1ml (for 5g substrate). The proposed model was highly significant as depicted by ANOVA. The results recommended that seed pods of Bombyx ceiba play important role in cellulase production, which is useful for the food industry, textile industry and ethanol product...
Abu ul Hassan Faiz1,*, Fakhar-i-Abbas2, Mehboob ul Hassan3 and Lariab Zahra Faiz1
...tudy site, line transect method for small mammalian pest, for large mammals spot light count and for birds point count methods were used to determine abundance of each species. The study, documented 10 species of mammalian pests, 13 species of pest birds of maize crop and their population density differed significantly (P≤ 0.05) at study sites. The habitat analysis of study sites and correlation of species with habitat we...
Muhammad Shoaib1,3*, Arif Ismiyev2,Khudaverdi Ganbarov3, Aygun Israyilova3 and Sajid Umar4
...i by agar well diffusion method. In second step, minimum inhibitory concentration of test compounds was determined against bacterial species by using resazurin microplate assay. All the tested compounds exhibited variable antimicrobial properties against various test cultures. All the compounds showed stronger antimicrobial activity against Gram-negative bacteria as compared to Gram-positive bacteria and fungi. Compound III was found to be the most effe...
Peng Chen1,2, Zuhao Huang3,Chaoying Zhu1, Yuqing Han1, Zhifeng Xu1
Guanglong Sun1, Zhen Zhang1, Dongqin Zhao4, Gang Ge1 and Luzhang Ruan1*
...xt-generation sequencing method to obtain mitochondrial DNA (mtDNA) of Porzana paykullii, Rallus aquaticus and Gallirallus striatus in Gruiformes, and Hydrophasianus chirurgus in Charadriiformes, after which we analysed and compared structure, phylogeny, and taxonomic origin of the Gruiformes and Charadriiformes. Based on sequencing, splicing, and annotating the mtDNA of four birds, the results showed that the lengths of mtDNA were ...

Mahnoor Pervez and Farkhanda Manzoor* 

...lected through capillary method allied with centrifugation. The HPLC technique was used for fast and simple detection of pesticide residues of commonly used pesticides (imidacloprid, deltamethrin, chlorpyrifos, fipronil, thiamethoxam, carbaryl, profenophos and bifenthrin). Among a total of 100 pollen samples, 47% were found to be positive. The most frequently found residues were imidacloprid (11%), thiamethoxam (7%) and carbaryl (6%). Among all 100 nectar samp...

Turab Ali Kaurajo1*, Huma Rizwana1, Gulbahar Khaskheli2, Muhammad Haroon Baloch1, Muhammad Naeem Rajput1, Asad Ali Khaskheli3 and Mohsin Solangi

...by using old traditional methods. The modern farming does not exit and extension services are rarely available to motivate and educate the camel herders. Higher load carrying capacity, hair production and body weight was observed in Kharai breed, however; milk production and lactation length was better in Dhatti breed. 

...

Manzoor1*, Ahmad Khan1, Amir Sohail2, Shahzad Ali1, Fawad Ali Shah3, Junaid Iqbal3, Muhammad Owais Khan4 and Sultan Nawaz4 

...le of different planting methods (Ridge, Flat and Broadcast) in soil moisture conservation for maize crop. The research was carried out using RCBD with split plot arrangement having 4 replications at Agronomy Research Farm, The University of Agriculture Peshawar, during May 2017. Deficit Irrigations were allotted to main plots, while planting methods were allotted to sub plots. Deficit irrigations had significant (P≤0.05)...
Javeria Zafar1, Asif Nadeem1*, Maryam Javed1, Fehmeeda Fatima1, Wasim Shehzad1, Ghulam Abbas1, Rajput Zahid Iqbal2 and Muhammad Muddassir Ali1
...ideoxy chain termination method after mitochondrial ATPase8/6 genes amplification. Polymorphism, Genetic diversity and Phylogenetic analysis were carried out by the MUSCLE, DnaSP and MEGA6 tools. Total of 20 variations at different positions were found in aligned sequence results. Sequence conservation was observed for the ibex population. The overall results showed a close evolutionary relationship among Capra aegagrus, Capra nubiana, Capra falconer...
Zübeyde Kumbıçak1,* and Hatice Poyraz2
...lassical Giemsa staining method. We collected specimens from different populations in Nevşehir and used totally fourteen male spiders. The two Drassodes species had karyotypes comprising 10 pairs of autosomes plus sex chromosomes which were X1X20 (♂) type. All chromosomes including sex chromosomes were telocentric and relative lengths of autosomal pairs were decreased gradually in size. All male meiosis was chiasmate and during ...

Urooba Pervaiz1*, Madeeha Iqbal1 and Dawood Jan2 

...ion personal, result and method demonstration, farm/home visit and personal meetings. Whereas, the rest were not satisfied with radio programs, television programs, exhibitions, pamphlets, and farmer day. It was concluded that production of maize in the study area was increased up to 910 kg/acre after extension recommendations. In case of wheat production was increased from 1177.50 kg/acre after extension recommendations to 1806.25 kg/acre. 

...

Rahat Naz*, Mussawar Shah, Asad Ullah, Intikhab Alam and Younas Khan 

... proportional allocation method. The conceptual framework comprised of two study variables i.e “agriculture production” and “climate change effects on human life”. To established association between the study variables the perception of climate change effect on human life was indexed and cross tabulated with local response to agricultural production at bi-variate level, using chi-square test statistics. The study found that climate chan...
Ammara Blouch1, Ata ul Mohsin2, Muhammad Naeem2 and Rashid Mahmood1*
...onditions using leaf dip method. Mortality was recorded after three and seven days of exposure. The results indicated that larval mortality increased with time and Turex (Bt sub speciess kurstaki and aizawai)after 3 days of exposure caused significantly higher mortality i.e 46.43, 43.45 and 38.69 % as compared to Dipel (Bt sub speciess kurstaki) that caused 19.05, 6.55 and 4.76 % mortality for 1st, 2nd a...
Chiping Kong1,2, Yongheng Wu1,2, Shangling Lou1, Benping Chen3, Simon D. Dowell4 and Yiqiang Fu1*
...an, China. We used three methods to compare the acoustic difference among and within individuals. The results demonstrated that male Emei Shan Liocichlas had individually distinct songs and can be used to identify individuals. We also found that 4 to 5 songs were relatively certain of an individual identity. Our findings indicated that individual distinctiveness by vocalisation was reliable in the Emei Shan Liocichla. Monitoring song therefore provides a poten...

Awais Ahmed Khan1*, Zafar Iqbal1 and Muhammad Atiq2 

...d to develop alternative methods to synthetic fungicides, currently used to control green mold of citrus caused by the Penicillium digitatum. Antifungal activities of organic elicitors were evaluated and all the elicitors expressed significant results. Salicylic acid was the most effective to control the disease and showed minimum mycelial growth diameter (35mm) followed by benzoic acid (44mm), jasmonic acid (45.5), and ascorbic acid (50mm) as compared to cont...
Romaan Hayat Khattak1,3, Hussain Ali1, Ejaz Ur Rehman1 and 
Muhammad Ali Nawaz1, 2*
...d Capture Mark Recapture method (CMR). A population of 834 ±356 were estimated in Shimshal and Socterabad CCHAs, with an estimated density of 0.6 sheep/km2. The present study showed a female-biased sex ratio (80 male/100 females), probably due to ongoing trophy hunting. The young to female ratio (0.5) indicated ongoing recruitment of young. The trophy hunting operates on a sustainable use approach, as limited animals are harvested to gen...
Hafrijal Syandri1*, Ainul Mardiah2, Azrita3 and Netti Aryani4

 

...are a viable alternative method as standard ponds for the commercial production of gurami sago.
...

Muhammad Sohail Memon1*, Khadim Ullah1, Altaf Ali Siyal1,2, Naimatullah Leghari1, Ahmed Ali Tagar1, Khalil Ahmed Ibupoto1, Syed Tahir Ata-ul-karim3, Muhammad Tahir4 and Noreena Memon1 

...ed bed-furrow irrigation method has increased in due to its capability of saving massive amounts of irrigation water. Knowing the importance of raised bed-furrow irrigation (RBFI) in water conservation and salt distribution in profile with three testing treatments viz. T1 with raised bed size = 0.6 m, T2 with raised bed size = 0.8 m and T3 with raised bed size = 1.0 m. The soil samples were collected from depth desired depth to analysis the Physical chemical p...

Muhammad Qaisar Nawaz*, Khalil Ahmed, Ghulam Qadir, Muhammad Rizwan, Muhammad Faisal Nawaz and Muhammad Sarfraz 

... the cost-benefit sowing methods for turnip production under moderately salt-affected soils. The experimental design was split-plot with sowing methods (bed and ridge) in the main plot and nitrogen doses (40, 60, 80 and 100 kg ha-1) as sub plot. Treatments were replicated three times with sub plot size of 4 m × 6 m. Measurements included were: number of plant/m-2, number of leaves/plants, root length, bulb diameter, fo...

Qaisar Mehmood1*, Maqbool Hussain Sial1, Saira Sharif1, Abid Hussain2, Muhammad Riaz3 and Nargis Shaheen4 

...x-Jenkins’s (1976) methodology. Secondary data, “50 years of Pakistan: volume-iii (1947-1997)” published by Pakistan Bureau of Statistics (PBS) and World Development Indicators World Bank (2016) from the year 1947- 2016 was used. After comparing all possible ARIMA model diagnostically, ARIMA (2, 1, 3) is the most parsimonious model with less forecast error. Forecast values for the fisheries production from 633.974 to 720.196 tons for the year...

Saleha Ashfaq1, Manzoor Hussain1, Nazish Bibi2, Jan Alam1, Muhammad Junaid2 and Sabi-Ur-Rehman3*

...sing agar well diffusion method against bacterial and fungal strains, while cytotoxic activity was studied in the methanolic extract using brine shrimp’s lethality assay. All the extracts showed significant biological activity against Gram positive, Gram negative bacteria and selected fungal strains. Acetone, chloroform and methanolic extracts showed maximum activity i-e 39mm, 44mm and 35mm followed by ethyl acetate 27mm and n-hexane 26mm against microor...
Nurlybay Kazhgaliyev1*, Talgat Kulmagambetov2, Dulat Ibrayev1, Saule Bostanova1 and Zhanat Titanov1
...tigate common zootechnic methods, daughters of imported Aberdeen Angus and Hereford cows were grouped at the age of 18-19 months with a live weight of no less than 350 kg. Groups were constituted using the analogue-pairs method and consisted of 30 cattle/group. Observations during the present study showed that the average live weight of Hereford heifers of the second generation at the age 18-22 months was 378.5 and 375.2 kg ...
Monica Paula Marin1, Elena Narcisa Pogurschi1*, Iuliana Marin2 and Carmen Georgeta Nicolae1
... natural, less expensive methods for improving the milk quality are the most studied by farmers and researches. Clinoptilolite, natural zeolite added to dairy cows diets, has been proven to be an efficient and economic solution. Starting from the fact that natural zeolites are recognized as having beneficial effects on the animal organism, being detoxifiers, immunomodulators, regulators of the internal pH, we studied the effect of using volcanic stuff as a foo...

Farheen Shafique1, Shaukat Ali2,*, Saiqa Andleeb1, Abdul Rauf1,3, Syed Ayaz Kazmi1,3, Sadia Idrees4, Faisal Farooq1,3, Faiq Nawaz Khan3,4, Muhammad Saad-ul-Hassan1,3, Raja Awais Mumtaz1,3, Saba Khalid1, Zaheem Ashraf1, Hafiz Muhammad Tahir2 and Fazal-ur-Rehman5

...ed through questionnaire method. After taking written consent from each participant or guardian, 5 ml of blood was collected from each participant and brought to the working laboratory for HBV and HCV screening through ICT kit method. All ICT positive samples were further confirmed through ELISA. Individuals 25(8.2%) were found positive for both hepatitis B surface Antigen (HBsAg) and Anti hepatitis C antibody (Anti-HCV anti...

Muhammad Israr1*, Helen Ross2, Shakeel Ahmad3, Nafess Ahmad4 and Urooba Pervaiz5

...esigned to develop a new methodological framework to measure multidimensional poverty that builds on the previous index for measuring three dimensions of poverty at the micro level, and a method to calculate the extent of poverty by using the Alkire and Foster model. The expanded dimensions of multidimensional poverty identified are wealth status, participation/empowerment, environment, health, education, and standard of liv...

Majid Khan, Naveed Jehan*, Muhammad Zahoor and Nadia Naz 

... Poverty Gap Index (PGI) method. The primary data was collected through structured questionnaire from 159 farmers. Monthly farmers’ income was calculated from the total crop and non-crop income. The results revealed that the poverty ratio and Poverty Gap index were 8.3 percent and 0.016 percent for the Virginia tobacco growers and 42.52 percent and 0.076 percent for the non-tobacco growers, respectively. This shows that the Virginia Tobacco growers pover...

Kalpana Polisetty1* and Kiran Kumar Paidipati2 

...prominent non-parametric methods i.e. Pettitt’s, Standard normal homogeneity (SNH) and Buishand’s range tests. The core purpose of these three techniques is to capture the mutation point in time series data connected to wheat production during the period 1980-2016. Consequently, Sen’s slope estimator has been used to measure the magnitude of the trend for wheat production and their significances examined through Mann Kendall (M-K) test. The a...
Nahed H. Ghoneim, Maha A. Sabry, Zeinab S. Ahmed and Esraa A. Elshafiee*
...d via the disc diffusion method and was observed most frequently to nalidixic acid (81.8 %), tetracycline (72.7 %), ciprofloxacin (54.5 %), and erythromycin (54.5 %), while low resistance to ceftriaxone (18.2 %) was detected. Among the 11 Campylobacter isolates, 9 isolates were multidrug resistant (MDR). The tet (O) gene, which is responsible for tetracycline resistance, was detected in only 6 isolates. Phylogenetic analysis of the tet (O)...
Ramazan İlgün1*, Nilgün Kuru2, Ferhan Bölükbaş3 and Fatih Mehmet Gür4
...ning electron microscopy methods. Six chicks (6-7 weeks), six layer hens (9-13 weeks) and six studs guinea fowl were used to study and determine the histological features of the tongue of the guinea fowl. Histological structures were examined using a light microscope the tissues and papillae of the tongue were examined with a the scanning electron microscope, and photos of the general histologic structures were taken. The tongue was triangular shaped, and cons...
Nihat Yeşilayer*
... visual and colorimetric methods, of three fish species. Linear relationships were observed between CIELAB colour measurements (lightness-L*, redness-a*, yellowness-b*, hue-H°ab and chroma-Cab), DSM SalmoFan card scores and TCCs. The redness (a*) was mostly correlated with TCC of the fish fillets. The FRT group had the highest TCC (9,75 mg kg-1) compared to the other two species. However, DSM SalmoFanTM card score was hi...
Nadeem Munawar1,2*, Tariq Mahmood2, Paula Rivadeneira1, Ali Akhter2 and Saqib Mehmood2
...ucted using the quadrate method to record the vegetation around rodents’ active burrows at field boundaries. The dominant wild vegetation included Cynodon dactylon, Saccharum griffithii, Dactyloctenium aegyptium, Dichanthium annulatum, Desmostachya bipinnata, Imperata cylindrical, Ziziphus nummularia, Achyranthes aspera, Calotropis procera, Sorghum halepense and Capparis deciduas. This vegetation supported the year-round popul...

Muhammad Ilyas1, Sardar Ali Khan1, Shahid Iqbal Awan1, Shafiq-ur-Rehman1, Waqar Ahmed1, Muhammad Riaz Khan2, Raja Mohib Muazzam Naz2, Muhammad Mohib Ullah Khan3 and Sumaira Hafeez1* 

...e quantitative biometric methods based on measurement of phenotypic performances of certain quantitative traits on as many as possible plant individuals in basic experimental generations. Initially, 108 inbred lines of maize were planted in two separate trails under water deficit and normal irrigation conditions in the experimental field of Department of Plant Breeding and Molecular Genetics. Selection of parents for further study was done with regards to grai...
Su-Hu Duan, Liu-An Li*, Zai-Qiang Li, Meng-Ran Qin, Zhen-Zhen Fan, Qian Wang, Ke-Yan Zhang, Zhong-Mou Zhang, Bai-Liang Yang and Tian-Ming Jin
...est, gas chromatographic method was used to determine the fatty acid content of ordinary eggs and native eggs of brands A, B and C. The results indicate that: In the test samples, totally 7 types of SFA, 7 types of MUFA, and 8 types of PUFA were detected. Specifically, no significant difference was found in the content of C14:0~C18:0 (P>0.05). The content of C16:0 in the yolk of both native eggs and ordinary eggs was high; in the yolk of native eggs ...
Chen Shi, Jianlong Zhang and Guanghui Teng*
...ody weight or growth day method. It also can provide a scientific foundation for precision feeding and automatic management of individual pig that could be applied in the real farm.
...
Muhammad Irfan Ullah1*, Muhammad Arshad1, Sajjad Ali2, Asad Abdullah1, Samina Khalid3, Hafiz Muhammad Aatif4, Syed Muhammad Ali Zahid1, Muhammad Afzal1 and Jaima Molina-Ochoa5
...s impartment. The recent methods used to quantify the foliar damage are relatively expensive and are mainly dependent on complex and state of art devices and instruments. Instead, smartphone application may be a good source to measure the foliar damage. In this study, foliar damage of Spodoptera litura Fab. (Lepidoptera: Noctuidae) on sunflower and soybean crops was measured using BioLeaf android application. The infected leaves due to citrus canker were also ...
Jun Yan Bai*, Hong Deng Fan, Shuai Yang, Xue Yan Fu, Heng Cao, Xiao Hong Wu, You Zhi Pang and Kun Peng Shi
...ail detected by PCR-SSCP method in this study. Moreover, correlations of control regions of MyoG 5’ with egg quality of quail were analyzed. Results demonstrated that: In egg quail group, six genotypes were detected in Locus A in the control region of MyoG 5’, including AA, BB, CC, AB, AC and BC. The C allele frequency at Locus A in China yellow quail, Beijing white quail and Korean quail was the highest (0.461, 0.528 and 0.579). A total of 9 genot...
Anila Naz Soomro1*, Hiroshi Suzuki2, Sadaf Tabassum Qureshi3 and Wazir Ali Baloch1
...mes; 1 mm) via the sweep method, sweeping efforts were counted. The results revealed that C. sakishiemnsis was restricted atSt. 1 only, a few juveniles of C. sakishiemnsis were recorded at St. 3, St. 4. The length frequency histograms showed the occurrence of large number of juveniles at St. 3, 4 and 5 in smaller length groups. Also C. typus ovigerous females exhibiting spawning migration to the downstream and its juvenile also migrate bac...
Syed Basit Rasheed*, Ahmad Yar, Farrah Zaidi and Qaisar Jamal
...fall traps and hand pick method. A total of 19 species representing 11 genera and three subfamilies namely Formicinae, Myrmicinae and Dolichoderinae were identified during the present study. Myrmicinae was the most diverse subfamily with five genera (Crematogaster, Meranoplus, Messor, Monomorium and Pheidole) and 11 species (Crematogaster subnuda, Meranoplus bicolor, Messor instabilis, Monomorium aberrans, Mo. dichroum, Mo. indicum, Pheidole b...
Meriem Msaad Guerfali1,*, Heitham Hamden1, Salma Fadhl1, Wafa Djobbi1, Lotfi Sillini1, Wafa Marzouki1 and Mohammed Ammar2
...ain conventional control method. Malathion upon repeated and prolonged use may cause resistance. In an attempt to reduce the insecticide residues in fruit, the government is trying to introduce the application of spinosad that was also reported to cause resistance under extensive use. The aim of this work was to monitor the susceptibility to malathion after prolonged use in wild caught tunisian Ceratitis
Larysa Kladnytska1, Anatoliy Mazurkevych1, Natalia Bezdieniezhnykh2Oleg Melnyc1, Sergiy Velychko3, Mykola Malyuk1, Vasyl Danilov1Yuriy Kharkevych1 and Magdalena Gryzinska4*
...dipose tissue by explant method. The cells were cultured in CO2 incubator by standard procedure. Expression of cytoplasmic and membrane proteins on dog stem cells from fat tissue at the IVth and Xth passages was examined by immunohistochemical method using monoclonal antibodies. Determination the index of proliferationdogs adipose-derived stem cells (DADSCs) on IVth and Xth

Ejaz Ashraf1*, Anam Sarwar2, Muhammad Junaid1, Mirza B. Baig3, Hafiz Khurram Shurjeel4 and R. Kirby Barrick

...criptive survey research method was employed in this census study. There were 147 working EFS and 130 of them participated in the study. A well-defined structured interview schedule was designed for data collection. The Borich Needs Assessment Model was applied on Microsoft Excel sheets to identify the training needs of EFS, and Statistical Package for Social Sciences (SPSS) version 20.0 used for additional analyses. The results indicated that humans are the c...
Renlong Lv1*, Ken-Ichi Horiguchi2, Shin-Ichi Tagawa3, Shoichi Ichihara4 and Norio Yoshida
...ed by using a cross over method. Rapeseed meal and jatropha meal were relaced to the feed as a concentrate with proportion of 5% in experiment I and 10% in experiment II. The ingestion rate of sheep fed the jatropha diet was lower than that of the rapeseed diet. The feed of jatropha diet and rapeseed diet were all ingested in experiment I. However, jatropha meal treatment left a large amount in experiment II. According to the above results, there was no obviou...

Fazal Haq*, Asia Parveen, Safdar Hussain and Arshad Hussain 

... figures so quantitative method of research is approached. The growers of the major crops were the targeted population and random sampling technique is used to select the sample. Self made questionnaires, filled through interviewed process from the random selected farmers were used as a tool for collecting primary data. The raw data is tabulated and analyzed using Microsoft Excel. The analyzed data is used to develop the mathematical model. Simplex
Aamir Mushtaq1,2, Rukhsana Anwar1 and Mobasher Ahmad1,2,* 
...micro;g/ml found by DPPH method) in plant extract. Higher inflexion ratio (0.25 ± 0.05), most of time spent in dark area (158.34 ± 4.88 sec), increased no of hole pokings (34.16 ± 2.01), significant (P < 0.001) elevation in superoxide dismutase (17.36 ± 0.49 U/mg), catalase (1.42 ± 0.05 U/mg) glutathione (37.56 ± 1.89 μ mol/mg) and reduction in malondialdehyde (...
Arifa Savanur1,*, Tallat Naz1,2, Tayyaba Hamid1,3, Syed Abid Ali4, Mian Jahangir1,3 and Muhammad Abdul Azeem4
...e quick isotonic release method was used for the measurement of stiffness in the series elastic component. The higher stiffness in intestinal tissues as compared to esophageal tissue of Uromastix is probably associated with its lesser compliance due to a lesser quantity of SEC that makes the intestine stiffer, as its muscle cells are less embedded in a collagen matrix. A significant difference between both types of muscle strips was observed in the rate...
Sohail Ahmed1,*, Muhammad Waseem Saleem1, Abid Ali1, Muhammad Shahid Nisar2, Rashad Rasool Khan1 and Abdul Rashid3
...ation with other control methods proved to be the most effective treatment. Releases of bio-agents viz., C. septempunctata and C. carnea resulted in 4.72 and 4.51 thrips leaf-1, respectively, and also did not differ significantly with each other. Application of bio-agents in combination was less effective with 4.37 thrips leaf-1 and differed significantly from all other treatments. Application of NKSE resulted in 4.00...
Xiuqing Hao1, Zhe Hong2, Huili Gao1, Fumin Tian1, Hongke Zhang3, Yu Zhou3, Yuming Guo3, Guang Yang1,* and Bingyao Chen1,*
...e utilized line-transect method to investigate the bird community and fishery vessel in Beihai and Shatian, northern Beibu Gulf, China, for obtaining the baseline knowledge. The best model generated the seabirds’ density of 11.47 (95% confidence interval 6.90-19.05) individuals/km2 and 1.94 (1.27 - 2.95) individuals/km2, respectively in Beihai and Shatian. The coefficient of variation value of 20.42% and 25.43% showed moderate accur...

Shah Nawaz Khuhro1*, Irshad Ali Junejo1, Muhammad Haroon Hullio1, Sultan Ahmed Maitlo2, Javeed Shabir Daar3 and Shahjahan Rajput

...lor traps may be used in methods of insect population monitoring. The other colors were less attractant so this study found that using yellow-colored sticky-traps as an alternative for Brinjal crop for the monitoring and management of whitefly. On the basis of results it is suggested that sticky traps are the most excellent integrated pest management approach to prevent problems from increase of whitefly population in the brinjal crop. Further is suggested tha...

Shah Nawaz Khuhro1*, Irshad Ali Junejo1, Muhammad Haroon Hullio1, Mohammad Farooque Hassan2, Sultan Ahmed Maitlo3 and Mukhtiar Ahmed Shaikh

...sing more on alternative methods for sustainable crop production and by reducing the use of pesticides and trainings on pest counting helps in sustainable pesticide usage in Pakistan. Randomly 66 farmers were selected to conduct the interview for background information about the growers’ status on pesticide application. The results show that the majority of the growers did not get any kind of trainings on sustainable use of pesticides. Results indicated ...

Ghani Akbar 

...e to traditional farming methods. Therefore, this paper is aimed to investigate the key agricultural issues and to identify strategies for utilizing the full potential of agriculture for improving livelihood in this region. This study indicated that climate change induced hazards of land degradation and water scarcity due to frequent floods and droughts, decline in traditional springfed Kuhl (gravity channel) irrigation system, low productivity of crops and ho...

Iram Fatima1*, Imran Pasha1, Ambreen Saddozai2, Shahid Nadeem3, Amer Mumtaz4 and Saqib Jabbar4 

...ted by Spread plate method. Prevalence of Total plate count checked and pathogenic count was calculated in CFU/g and CFU/ml, mean CFU for E. coli and TPC was (3.39×104 to 3.15×105). Total 47.9% samples of spices were found adulterated for eight different adulterants tested. Sugar samples were free of any adulteration while in beverages two other adulterants were found in 22% of samples. Analysis of variance (...

Wajiha Anum1*, Liaquat Ali1, Umair Riaz2*, Abid Ali1, Nadia Manzoor3, Laal Hussain Akhter4, Asad Ur Rahman5, Naeem Maan6 and Ijaz Ahmad

...t of various P placement methods and fertilizer rates on wheat. The fertilizer treatments were F1=150-00-60 NPK kilogram/hectare, F2=150-30-60 NPK kilogram/hectare, F3=150-60-60 NPK kilogram/hectare, F4=150-90-60 NPK kilogram/hectare, F5=150-120-60 NPK kilogram/hectare. While the placement methods were M1=Line sowing + Band application of phosphorus, M2=Line sowing +Broadcast application of Phosphorus at sowing, M3=Line sowi...

Ghani Akbar*, Muhammad Asif, Zafar Islam and Shahid Hameed 

...ional surface irrigation method, but has been shown inefficient under actual farm condition in Pakistan. The main reasons being attributed to lack of knowledge of furrow bed design, installation and management. Therefore, this study is aimed to illustrate appropriate installation and management of furrow bed irrigation method for enhancing decision support on farms. Pakistan is a major irrigated agricultural country with mor...
Hina Nazli, Abida Butt*
...cal and chemical control methods are required to successfully manage insect pests. Along with insect pests, many predators of these pests are also present in crops and affected by these management activities. Spiders are the most abundant predators of insect pests in the agroecosystem. The present study was designed to assess and compare acute and residual toxicity of two insecticides i.e Spiromesifen and Thiamethoxam on the lynx spider Oxyopes javan...
Fehmina Ashraf1*, Muhammad Irfan2, Hafiz Abdullah Shakir1, Shaukat Ali3, Muhammad Khan1
...been purified by various methods. It involves in di-oxygen to water reduction and 1e- oxidation of phenolic and its allied parts. Laccases are mostly used in different industries like food, paper and pulp, pharmaceutical and textile etc. Both laccases and mediators have been used in different process like delignification of pulp. Laccases from bacterial species are used in dye decolouration and biobleaching processes. This review article describes the whole ov...

Md. Al-Amin Tan1, Mst. Antora Akter1, Md. Sabuj Rahman1, Marzia Rahman2 and Md. Mahmudul Alam1* 

...f skin disinfectants and methods of skin preparation is still aberrant in veterinary practice. The study was, thus, conducted to standardize pre-surgical skin preparation with various formulations of Chlorhexidine gluconate, Chlorxylenol, and Povidone-iodine (PI) in goat. Seven surgical fields were prepared for evaluating seven formulations of these antiseptics. The bacterial swabs collected at different stages of skin preparations were transferred in nutrient...

Hassan Boulahyaoui1,2*, Sanaa Alaoui Amine1,3, Marouane Melloul4, Farida Hilali5, Elmostafa El-Fahime1,3, Saad Mrani1,2 and Nadia Touil1,5* 

...g the Maximum Likelihood method applying the Tamura 3-parameter model in MEGA 6.06 package and statistically supported by bootstrapping with 1000 replicates. The amino acid sequences of the antigenic regions of the outer capsid proteins VP8* were compared with vaccine and field strains. Here we show that the VP4 gene of the P[14] strains detected in this study exhibited close identity with zoonotic Moroccan goat and bovine strains. The amino acid sequences of ...

Joseph Anejo-Okopi1*, Ocheme Julius Okojokwu1 and Onyemocho Audu2 

...nd inexpensive molecular methods for accurate diagnosis. The sub-Saharan Africa HCV genotypes distribution are diverse, but genotype I is the most common. This review highlights the need for more robust surveillance studies with introduction of opt-in testing at all clinics for better epidemiology the HCV disease burden more accurately.  

...
Muhammad Usman Asif1*, Sadam Hussain Soomro2, Raza Muhammad1, Sikandar Ali Soomro2
...d high doses by leaf dip method. The results concluded that significantly high toxicity was shown after treatment with Neomed 3 SC which caused 100 % to the insect at its recommended (200 ml) and high dose (400 ml) after 24 hours of application followed by Voliam Flexi 300 SC causing 66.66 % and 75% mortality on its recommended (80 ml) and high dose (160 ml), respectively. After 72 hours, 83.33% mortality was observed in Voliam Flexi on its recommended dose wh...
Imad Hassan1, Bilal-ur-Rehman2, Muhammad Amir2, Faheem Ali2*, Salman Ilahi2 and Amjad Ali3 
...or (ESP) is an effective method for the removal of particulate matter from industrial processes and boilers. This research proposes the development of a cost effective, simple and compact ESP, through practical installation and efficiency analysis. Experimental evaluation of the proposed ESP design is carried out for the removal of particulate matter from a biomass combustor. The results shown in this paper are promising and exhibit that a simple solution can ...

Amjad Ali1, Awais Salman1, Gul Daraz Khan1, Aftab Ahmad Khan2, Sher Shah Hassan2*, Muhammad Arif Goheer2, Mahmood Alam Khan3 and Sajjad Ahmed

... through the gravimetric method. Results showed that different deficit irrigation levels and sowing dates significantly affected different growth parameters, yield, weight and harvest index of turnip. Maximum number of leaves per plant (19.16), leaf length (19.97 cm), leaf width (10.46 cm), plant height (39.27 cm), turnip length (17.24 cm), turnip diameter (7.84 cm), turnip yield (22.42 tons ha-1), turnip weight (256.62 gm) and harvest index (52.34 %) were re...

Rana Shahzad Noor1,2*, Fiaz Hussain2, Muhammad Umair2, Waqas Aslam2, Muhammad Ameen4, Muhammad Adnan Islam3 and Muhammad Sheraz2 

 

... under different tillage methods was 2097.8 kg ha-1, while the lowest yield was observed as 1649.9 kg ha-1 respectively. The fuel consumption of main crop was measured between 33.33 l ha-1 and 63.48 l ha-1, while second crop fuel consumption was ranged as 34.92 l ha-1 to 62.48 l ha-1. The highest and lowest fuel consumptions levels of different soil tillage for main and second crop peanuts were 62.61 and 34.08 l ha-1 and 33.09 and 61.82 l ha-1, respectively. T...

H. Soofi†1 , N. A. Birmani1 , A. M. Dharejo1 , A. R. Abbasi2 , G. S. Ghachal1

Description of new nematode species Rhabdochona (Rhabdochona) sindhicus of genus Rhabdochona (Railliet, 1916) from Indus River Pakistan
... processes with standard method for feature research. New species Rhabdochona (Rhabdochona) sindhicus differs from other species of genus with body shape and size, buccal capsule shape, prostomal teeth arrangement, nerve ring position, 18 pairs of caudal papillae, spicules shape curved and broad anteriorly and pointed posteriorly, female genital structures, shape of posterior end in male and female, eggs shape and boundary. Due to morphometric differences betw...

 K. Abbasi 1 , R. Wick2† and D. Zafari1

Chitinase activity of biocontrol fungi cultured from the golden potato cyst nematode, Globodera rostochiensis
...samine-dinitrosalicylate method. Ten isolates with high chitinase activity were selected for further characterization. Of the 10 selected isolates, three chitinase enzymes were evaluated using three substrates. Based on the results of our chitinase assay, Beauveria bassiana, Lecanicillium muscarium, Paecilomyces sp. and Trichoderma atroviridae had the highest activity. We selected these four isolates to determine the optimum pH, temperature, and reaction time....

Ahmad Hussain1*, Syed Murtuza Mehdi2, Amjad Ali3, Muhammad Adeel3, Mohammed Hassan Jabal4, Farid Nasir Ani4 

INVESTIGATION THE TRIBOLOGICAL CHARACTERISTICS OF CASTOR OIL WITH MINERAL OIL BLENDS
...wn four ball tribotester method. In the
experiments, castor oil is blended at volumetric ratio ranging from 20 to 80 % with commercial mineral oil, where
the setup is consictent with ASTM D4172. Results indicate that a mix of castor oil with commercial lubrication oil
rather than mineral oil, can bring down the diameter of wear scar. The mix exhibits a decrease in the coefficient of
friction when compared with lubrication oil. A bel...

Muhammad Ismail1, Shahid Maqsood1*, Abdul Shakoor2, Feroz Shah2 

DESIGN AND COST ANALYSIS OF MOLD MAKING FACILTY AT GHANI GLASS INDUSTRY: A CASE STUDY
...ut
Planning (SLP) method and efficiency rate is calculated. The cost analysis of the modified layout was calculated by
giving a payback period also. The results show that the proposed layout performs efficiently for the annual demand. 

...

Riaz Muhammad1*, Naseer Ahmeda, Abdul Basit2, Rizwan Alim Mufti2 

EXPERIMENTAL INVESTIGATION OF FRACTURE TOUGHNESS, HARDNESS AND LOAD-INDENTATION DEPTH RESPONSE OF TI-64 USING VICKERS INDENTATION TECHNIQUE
...y model. The indentation method resulted a fracture toughness
of the studied alloy with 14.5% when compared to the results obtained from strain dependent ductile damage model. 

...

Samreen Fatima1*, Mudassir Uddin1 

COMPARISON OF ASYMMETRIC GARCH MODELS WITH ARTIFICIAL NEURAL NETWORK FOR STOCK MARKETS PREDICTION, A CASE STUDY
...nd non-parametric
method (Artificial Neural Networks). In the A-GARCH; EGARCH and PGARCH models are used. Firstly, suitable
Asymmetric GARCH (A-GARCH) model was developed for forecasting and investigating leverage effect. Secondly,
an Artificial Neural Networks model was developed for the said stock markets. Lastly, forecasting performance of the
FA-GARCH and ANN models both in and out sample were evaluated using root mean square er...

Nasir Ahmad1*, Jehad Ali2, Khalil Khan3, Muhammad Naeem4, Usman Ali5 

ROBUST MULTIMODAL FACE RECOGNITION WITH PRE-PROCESSED KINECT RGB-D IMAGES
...r applicability of these methods is inadequate in practical implementations
due to high cost of 3D sensors, therefore we are using the low cost sensor Kinect acquired images. We do
face recognition from RGB images, depth images and then we combine both RGB and depth maps i.e. concatenate
different Modalities to improve the accuracy of recognition. Depth maps have holes and noise induced from camera
sensors, therefore we process them...

Muhammad Shahzad Khattak*1, Barkatullah1, Ayesha Aziz1, Jamil Ahmad2, Mohammad Sharif3, Mukand Singh Babel4, Muhammad Fahad5

IMPACTS OF CLIMATE CHANGE ON CROP WATER REQUIREMENT UNDER MULTI - REPRESENTATIVE CONCENTRATION PATHWAYS DURING MID-CENTURY: A CASE STUDY OF D. I. KHAN
...ion Pathways (RCPs). The methodology employed here involves
the comparison of temperature and precipitation data projected by different GCM with the observed data. The delta
change and ratio method was used to obtain the corrected value of temperature and Precipitation (PPT) for the
future. The ETo calculator of Food and Agriculture Organization was used to calculate evapotranspiration. The CWR
of...

Misbah Ullah1, Rehman Akhtar1, Sikandar Bilal Khattak1, Khawar Naeem1 and Khizar Azam2 

FACTORS IDENTIFICATION FOR COAL BRIQUETTES MANUFACTURING USING STATISTICAL EXPERIMENTAL DESIGN APPROACH
...red through conventional methods in developing countries including Pakistan.
Shortages of energy resources across the globe have pushed companies to manufacture those products with least
possible costs by using significant factors. Indeed, the role of statistical design of experiment in general and factorial
design in particular is vital in identifying these significant factors in comparison to the conventional techniques. This
pape...

Kulsoom Iftikhar1, Shahzad Anwar1, Izhar Ul Haq1, Muhammad Tahir Khan1 and Sayed Riaz Akbar1 

AN OPTIMAL NEURAL NETWORK BASED CLASSIFICATION TECHNIQUE FOR BREAST CANCER DETECTION
...efficient classification method
for benign and malignant breast cancer. The proposed method employs an optimal feature classification employing
artificial neural network. The proposed architecture has five input nodes, two hidden layers with eight neurons each
and one output node. Five features (cluster thickness, uniformity of {cell size, cell shape}, marginal attachment and
radius of circle encl...

Tehmina Amjad1, Ishfaq Ahmad1, Muhammad Akram Shaikh2, Nabila Naz1 

VARIATION IN CITATION BASED FRACTIONAL COUNTING OF AUTHORSHIP
...

...

Nazish Huma Khan1, Mohammad Nafees1, Adila Bashir1, Farooq Ahmad1 

STUDY OF POLLUTION LAOD IN PAPER MILL EFFLUENTS AND ITS RECYCLING BY SUNDRY PLANTS AT HAYATABAD INDUSTRIAL ESTATE, PESHAWAR
...D5) by adopting standard methods for the
examination of water and waste water. These samples were also investigated for micronutrients/heavy metals (Cr, Pd,
Cd, Ni, Fe, Zn and Cu), using Atomic Absorption Spectrophotometer. The obtained results were compared with Pak-
NEQS for industrial effluents. Results showed high limits of pH(8-8.2), EC(738-764uS/cm), Alkalinity(463-581mg/l),
Turbidity(166-180NTU), TSS(967-1063.5mg/L), TDS(1673...

Shabana Rafique1, Shahnaz Parveen Khattak1, Tanveer Hussain2, Faiza Tauqeer1, Bashir Ahmad3 

EVALUATION OF FLEXURAL RIGIDITY AND ABRASION RESISTANCE OF POST AND META-FINISHED PIGMENT DYED P/C FABRICS
...omical than conventional method, since, more
energy can be conserved by using same machinery for dyeing, finishing, drying and curing. Furthermore, elimination
of post wash treatment in this method caused little or no contamination. Despite its manifold advantages, the rubbing
fastness and fabric stiffness of pigment dyed fabrics in deeper shades is doubtful, hence needed further exploration.
The ...

Rahim Haider*, Al Mustaan Kakar*, Sikandar Bilal Khattak*, Safi ur Rahman**, Shahid Maqsood*, Misbah Ullah*, Rehman Akhtar*, Aamir Sikandar* 

DEVELOPMENT OF OPTIMIZED MAINTENANCE SYSTEM FOR VEHICLE FLEET
...es the inspection
method by using checklists and maintenance schedules. The system is capable to provide the basis for an advance
system development. 

...

Suhaib*, Shahid Maqsood*, Sikandar Bilal Khattak*, Misbah Ullah*, Rehman Akhtar*, Rashid Nawaz*, Iftikhar Hussain*, Abdul Shakoor**, Khizar Azam** 

SINGLE FACILITY LOCATION SELECTIONPROBLEM FOR A PAKISTAN BASED ICE CREAM COMPANY: A CASE STUDY
...ization technique median method is applied to a
real world problem of a multinational ice cream company’s factory located in Lahore, Punjab (province) Pakistan,
with an objective of minimum overall cost between a warehouse and destinations. The company wanted to locate the
warehouse in Khyber Pakhtunkhwa (KPK), province in order to meet the demand in KPK on time and reduce the
travelling cost between the warehouse and destinat...

Hassan Nasir*, Rashid Rehan,** Rizwan Younis,*** Fazal Mabood,**** Salman Saeed,**, Muhammad Sagheer Aslam** 

MACHINERY SELECTION FOR DEMOLITION AND RECYCLING OF PAVEMENTS: A SIMULATION-BASED TOOL
...e
system dynamics methodology to help the construction manager in investigating the cost and duration implications of
various combinations of machinery items. Utility of the developed model is illustrated with the help of a case study
involving demolition and recycling of a flexible pavement. 

...

Muhammad Asad Ali, Mirza Jahanzaib, Muhammad Ahsan Ul Haq 

ANALYZING THE EFFECT OF SQUEEZE CASTING PROCESS PARAMETERS ON MECHANICAL PROPERTIES OF OVERCAST AL-ALLOY JOINT USING RSM
...rature. Response surface methodology (RSM) was employed to analyze the effect of above mentioned input parameters on UTS and YS. Empirical models for UTS and YS were developed which help the practitioners to achieve desired UTS and YS by using optimum vales of input parameters. ANOVA results shows that melt temperature has most significant effect on UTS and YS followed by squeeze pressure and pressure duration. Current study will provide efficacious approach t...

Waqas Anwar, Iftikhar Hussain 

COMPARATIVE STUDY ON CONSTRUCTION PROJECT MANAGEMENT BETWEEN RESTIVE REGIONS OF PAKISTAN AND REMAINING PART OF COUNTRY
...jects. The author adopts methodology of quantitative analysis using SPSS for drawing important conclusion from research. 

...

Mujahid Badshah, Saeed Badshah, Sakhi Jan 

HYDRODYNAMIC DESIGN OF TIDAL CURRENT TURBINE AND THE EFFECT OF SOLIDITY ON PERFORMANCE
...e (HATCT). The numerical method was validated with the experimental data and a good agreement was achieved. The designed turbine has a 3 bladed rotor of 4 meters diameter and rated mechanical power of 20 kW. Performance metrics of the rotor for steady and uniform flow was simulated at flow speeds from 0.5-3.5 m/s. The turbine achieved its rated power at a tip speed ratio (TSR) 5.7 with a peak CP value of 0.47 and peak thrust of 16.7 kN-m. Additionally, a serie...

Waqas Anwar, Iftikhar Hussain 

COMPARATIVE STUDY ON CONSTRUCTION PROJECT MANAGEMENT BETWEEN RESTIVE REGIONS OF PAKISTAN AND REMAINING PART OF COUNTRY
...jects. The author adopts methodology of quantitative analysis using SPSS for drawing important conclusion from research. 

...

 Mahmood Alam Khan, Afzal Munir, Muahmmad Shahzad Khattak, Daulat Khan, Muhammad Ajmal

GROUNDWATER RECHARGE QUANTIFICATION FROM RAINFALL IN PESHAWAR DISTRICT-KHYBER PAKHTUNKHWA
...ce Curve Number (SCS-CN) method was used to estimate the runoff produced from rainfall events. As per the data provided by Pakistan Metrological Department, Peshawar, average annual rainfall in district Peshawar during 2002-2011 was 486.1 mm. The weighted curve number (CN) was found to be 82.0. Mean annual runoff produced was estimated to be 217.0 mm where as mean annual recharge to the groundwater was calculated and found to be 269.1 mm. Different water cons...

Asif Nawaz1*, Muhammad Zafarullah Khan1, Rehmat Ullah1, Arshad Farooq2 and Abdul Hassan2

Assessment of Weed Management Competency of Field Assistants in Khyber Pakhtunkhwa, Pakistan
...t indirect weeds control methods. Moreover, it was found that Field Assistants required highest training needs in familiarity with biological weed control followed by critical threshold level of weeds and critical period of weed competition. Findings of Rank Based Quotient revealed that lack of promotion, training opportunities and incentives or motivation were the top most constraints that respondents faced in building the required competencies. Therefore, it...

 Akhtar Nawaz*, Sahar Noor*, Iftikhar Hussain*, Afzal Khan*, Shahid Rashid**, Rehman Akhtar*

METHODOLOGY FOR POSITIONING MULTIPLE DECOUPLING POINTS IN HYBRID MANUFACTURING SUPPLY CHAIN SYSTEM
...iv>
and integrated methodology for designing interface for hybrid manufacturing supply chain networks for quick
response to the delivery lead time.
...

 Nasimullah*, Muhammad Irfan Khattak**, Muhammad Shafi**, Gulzar Ahmad**

A TUTORIAL ON ADAPTIVE ROBUST POSITION CONTROL OF DC MOTOR USING FUZZY LOGIC COMPENSATION
...mode
control method. Adaptive fuzzy system formulated using Lyapunov function. The proposed controller is derived for
DC motor position control system and numerical simulations are presented to verify its effectiveness.
...

 Muhammad Asad Ali 1*, Mirza Jahanzaib1, Muhammad Ahsan Ul Haq1

ANALYZING THE EFFECT OF SQUEEZE CASTING PROCESS PARAMETERS ON MECHANICAL PROPERTIES OF OVERCAST ALUMINIUM-ALLOY JOINT USING RESPONSE SURFACE METHODOLOGY
...rature. Response surface methodology (RSM) was employed to analyze the effect of
above mentioned input parameters on ultimate tensile strength (UTS) and yield strength (YS). Empirical models for
UTS and YS were developed which help the practitioners to achieve desired UTS and YS by using optimum vales of
input parameters. ANOVA results shows that melt temperature has most significant effect on UTS and YS followed

Waqas Anwar1*, Iftikhar Hussain1

COMPARATIVE STUDY ON CONSTRUCTION PROJECT MANAGEMENT BETWEEN RESTIVE REGIONS OF PAKISTAN AND REMAINING PART OF COUNTRY
...uthor adopts
methodology of quantitative analysis using SPSS for drawing important conclusion from research.
...

 Mujahid Badshah*1, Saeed Badshah1, Sakhi Jan1

HYDRODYNAMIC DESIGN OF TIDAL CURRENT TURBINE AND THE EFFECT OF SOLIDITY ON PERFORMANCE
...e (HATCT). The numerical method was validated with the experimental data and a good agreement
was achieved. The designed turbine has a 3 bladed rotor of 4 meters diameter and rated mechanical power of 20
kW. Performance metrics of the rotor for steady and uniform flow was simulated at flow speeds from 0.5-3.5 m/s.
The turbine achieved its rated power at a tip speed ratio (TSR) 5.7 with a peak CP value of 0.47 and peak thrust...

 Mahmood Alam Khan1*, Afzal Munir1, Muhammad Shahzad Khattak1, Daulat Khan1, Muhammad Ajmal1

GROUNDWATER RECHARGE QUANTIFICATION FROM RAINFALL IN PESHAWAR DISTRICT-KHYBER PAKHTUNKHWA
...ce Curve Number (SCS-CN) method was used to estimate the
runoff produced from rainfall events. As per the data provided by Pakistan Metrological Department, Peshawar,
average annual rainfall in district Peshawar during 2002-2011 was 486.1 mm. The weighted curve number (CN) was
found to be 82.0. Mean annual runoff produced was estimated to be 217.0 mm where as mean annual recharge to the
groundwater was calculated...

 Muhammad Khurram Ali*, Raft Javed Qureshi**, Mirza Jahanzaib***

SELECTING FACILITY LOCATION USING HYBRID METHODOLOGIES IN GLOBAL PERSPECTIVE: A CASE OF CEMENT PLANT
...appropriate optimization methodology,- compatible to the said problem. This paper addresses a real world case study of the problem of selecting a country best suitable for a new cement plant installation among the list offour alternatives. The irrevers...

M. Rizwan Gul*

COMPARISON OF THE OF MECHANICAL AND WEAR BEHAVIOURS OF DIFFERNT TYPES OF POLYETHYLENES AND THE EFFECT OF RADIATION CROSS-LINKING ON THESE BEHAVIOURS
...e of the most convenient methods. This study explores the difference in mechanical and wear behavior of different polyethylenes, and compares the effect of radiation cross-linking and radiation dose on these properties. Three different types of polyethylenes: low density (LDPE), high density (HDPE), and ultra-high molecular weight (UHMWPE) were studied. Cross-linking was carried out by high energy electron beam at room temperature, with radiation dose ranging ...

M A Khalid*, A R Ghumman**, H. N. Hashmi**, E. Tanveer*

INVESTIGATION OF PERFORMANCE AND SEDIMENT TRANSPORT OF LOWER BARI DOAB CANAL
...by the finite difference method. Numerical models can be used for this purpose. SETRIC model has been used in this paper. The sediment deposition and erosion in time and space along the canal has been simulated. The relevant data were obtained from Punjab Irrigation and Power Department Lahore. It is observed that sediment deposition and erosion is a significant problem being faced by the canals in Punjab.
...

Muhammad Waseem*, Irshad Ahmad**, Rawid Khan**, Bashir Alam**, Khan Shahzada**, Muhammad Javed**

STABILITY ANALYSIS OF SOIL SLOPE IN SWABI, PAKISTAN
...as carried out using the method of slices. Gravity load and seismic load were considered in the stability analysis. SLOPE/W and SIGMA/W software of Geo studio package were used for analysis under both dry and completely saturated conditions. Pseudo-static approach was used for the seismic stability analysis. The peak ground acceleration value for stability analysis was obtained from the seismic hazard analysis. Two soil slope conditions were considered, and on...

Muhammad Imran Ahmad*, Nan Zhang**, Megan Jobson**, Muhammad Younas*, Kamran Ghani***

DELUMPING PROCEDURE FOR PREDICTION OF DISTRIBUTION OF PRODUCTS IN DISTILLATION USING A SHORT-CUT MODEL
...nceptual design stage. A methodology for prediction of detailed product distribution for distillation processes using short-cut calculations of distillation is developed in this work. The proposed delumping procedure is explained in this paper, and an illustrative example is presented to elucidate the application in modeling of distillation using short-cut calculation methods. The proposed

Gulzar Ahmad*, Sahibzada Faheem*, Amjad Ullah*, Waqar Shah*, S.M. Majid Ashraf*

COMPARATIVE STUDY OF RELAY PROTOCOLS USING SIMULATION
...mal ratio Combiner (MRC) method by combining signals from source and relay at destination point. The transmission path is modeled as a frequency non-selective Rayleigh fading in the presence of additive white Gaussian noise (AWGN). The results show that at high SNR decode-and-forward performs well, while at low SNR amplify-and-forward works well. A selective relaying scheme called selective detect-and-forward is also simulated which shows b...

Muhammad Naeem*, Sohail Asghar**

A REVIEW OF FEATURE SELECTION TECHNIQUES IN STRUCTURE LEARNING
... corresponding searching methods under six well known scoring functions in K2 which is a notable structure learning technique in Bayesian belief network. We have come up with some useful findings for overall computationally efficient approach among eleven evaluators. This analysis is useful in inducing better structure from the given dataset in imparting improved performance metric for Bayesian belief network.
...

Mumtaz Shah*, Hashim Nisar Hashmi* Naeem Ejaz* Abdul Razzaq Ghumman*

PERFORMANCE EVALUATION OF AQUATIC MACROPHYTES FOR MUNICIPAL WASTEWATER TREATMENT
...wage treatment, the method found to be environmentally and financially sustainable.
...

Shahid Ali1, Habib Akbar2, Shamsher Ali3*, Adnan Nasim1, Muhammad Ismail1, Nur Ul Haq1 and Muhammad Usman4

Effect of Planting Sources, Cane Portions and Setts Placement Methods on Sugarcane Yield Attributing Traits
... fresh stocks, efficient method of setts placement, selection of paramount cane portions and the interaction of yield and yield components. The effect of planting sources (i.e. sugarcane fresh seed and the one obtained from stock buried underground), cane portions and setts placement methods on sugarcane yield attributing traits were examined at Sugar Crops Research Institute (SCRI), Mardan, Khyber Pakhtunkhwa Pakistan. The ...

Sajad Ali1*, Jangraiz Khan2, Arshadullah Jadoon3, Muhammad Riaz4 and Abid Khan1

Evaluation of Farmers Socioeconomic Characteristics Influencing Tomato Output in District Peshawar, Khyber Pakhtunkhwa, Pakistan
... technique and interview method were adopted for data collection. A sample of 160 tomato (Kharif) growers was drawn from 16 villages of district Peshawar. Chi-Square and Pearson Rank correlation tests were employed to find out the association and correlation between level of productivity and socio-economic characteristics of tomato growers such as education level, work involvement, family size, farming experience, age, and source of income and land size. The r...

Ejaz Ashraf1*, Hafiz Khurram Shurjeel2, Saima Sadaf1, Adnan Ahmad1, Usman Rafique1 and Muhammad Arshad Javed1

An Assessment of Farmers’ Awareness Level Regarding Integrated Farming System in District Sargodha, Punjab, Pakistan
...tity of food produced by methodical exploitation of resources. Purpose of the study was to assess the awareness of the farmers to determine the impact of integrated farming system (IFS) on agricultural development and improving the livelihoods in the study area. To accomplish these objectives, an interview schedule was developed as a research instrument to record the opinions of the respondents by using Likert-type scale. The 200 respondents were selected thro...

Ejaz Ashraf1*, Hafiz Khurram Shurjeel2, Saima Sadaf1, Adnan Ahmad1, Usman Rafique1 and Muhammad Arshad Javed1

An Assessment of Farmers’ Awareness Level Regarding Integrated Farming System in District Sargodha, Punjab, Pakistan
...tity of food produced by methodical exploitation of resources. Purpose of the study was to assess the awareness of the farmers to determine the impact of integrated farming system (IFS) on agricultural development and improving the livelihoods in the study area. To accomplish these objectives, an interview schedule was developed as a research instrument to record the opinions of the respondents by using Likert-type scale. The 200 respondents were selected thro...

Abroon Jamal Qazi*, Afzal Khan*, Muhammad Tahir Khan*, Muhammad Ali Kamran* and Khizar Azam*

A NOVEL APPLICATION OF PARTICLE SWARM OPTIMIZATION TECHNIQUE IN SEMI-ACTIVE SUSPENSION SYSTEM CONTROL
...ticle swarm optimization method is utilized for its better convergence and precision. The technique results in evaluation of appropriate gains that result in superior performance of the designed system. The models are compared on the basis of suspension displacement and tire displacement for ascertaining ride comfort and road handling attributes respectively.
...
Muhammad Shaheen
APPLICATION OF LABELLED K MEANS CLUSTERING FOR GIS CONTRACT AUTOMATION
...is paper proposes (1). a method to regulate the technical part of the GIS contract by suggesting a simple and wizard-based Graphical user interface. (2). Conversion of existing manually prepared contracts into electronic contracts through lexeme-based congregation which is done through labelled K Means Clustering. These converted clusters are then stored into centralized database. Back Propagation Neural Network (BPNN) is used to train the system on perfo...

 Gulzar Ahmad1, M. Inayatullah Babar2

AN EFFICIENT PATCH ANTENNA WITH IMPROVED BANDWIDTH FOR FUTURE APPLICATIONS
...s 1.64mm. Different methods were engaged to expand its bandwidth from 9.07GHz to 13.13GHz. The reflection coefficient of -45dB at the central frequency, a gain of 7dBi and total efficiency of 0.99 have been demonstrated by this proposed design of the patch antenna. This novel structured wide band and high efficient antenna can be engaged in various types of wireless applications in near future.

...

 Saman Shahid1, Shahid Ali1, Muhammad Abdullah Ahmad1, Sarfraz Munir2

RE-DESIGNING AND OPERATIONAL ANALYSES OF THE SEDIMENT TRANSPORT CONTROL IN IRRIGATION CANAL NETWORK
...ngelund Hansen predictor methods. The data related to BRBD canal, including its cross-sectional properties, hydraulics and sediment parameters were acquired from the Irrigation Department, Lahore. The paper also described the problem which exists in design and operation of the canal by comparing it with original field data. The BRBD canal achieved a regime condition in 1.5 years. The results of sediment deposition produced by Brownlie predictor were more accur...

 Nashitah Alwaz1, Safdar Raza1*, Sadaqat Ali1

A COMMUNICATION-FREE SIGNAL GENERATING TECHNIQUE FOR REACTIVE POWER CORRECTION IN ISLANDED MICROGRID
...mands. The droop control methods are normally used for appropriate power sharing. However, these control techniques suffer badly due to the feeder impedances and uncontrollable power coupling issues. Many techniques have been proposed to share power accurately, most of them rely on communication
link. In this work a localized/communication free signal generating mechanism is proposed. The mechanism injects&nbs...

Altaf Hussain Rajpar1, Abdul Hameed Memon1, Mohammad Akram Shaikh2, Weimin Zhang3

HAND-EYE COORDINATION FOR OBJECT GRASPING TASK OF HUMANOID ROBOT (BHR-02)
...ask of humanoid robot. A method proposed for hand-eye coordination addresses four distinct problems i) Integration of vision control and motion control ii) hand-eye coordinate transformation iii) orientation of hand with target object iv) hand-eye localization. For hand localization and to limit the orientation problem a blue colored rectangle block was pasted on the robot hand. The blue colored rectangle block four sides were detected by a...

 Nisar Mohammad1, Mohammad Mansoor Khan1

RECOVERY OF TALC FROM TALC- CARBONATE SCHIST OF SWAT EMERALD MINES, NWFP, PAKISTAN
...ration and leaching methods were conducted to see the amenability of talc to enrichment by these techniques. Analysis of seven talcum powder products available in the local market including pharmaceutical grade talc was carried out for comparison with Swat raw- talc under study and to know the level of impurities in it. The results revealed that Swat emerald mines talc rock contains calcite, magnesite, dolomite, muscovite and quartz as impu...

Amjad Ali1, Hong Luo1, Khalid Saifullah Syed2, Muhammad Ishaq2

A PARALLEL DISCONTINUOUS GALERKIN CODE FOR COMPRESSIBLE FLUID FLOWS ON UNSTRUCTURED GRIDS
.... The parallel numerical method is capable to efficiently simulate the flow physics of the problems considered, including subsonic and transonic compressible inviscid fluid flows around two well known benchmark airfoils. The parallel discontinuous Galerkin method is presented for distributed memory parallel computing architectures, specifically considered cost effective compute clusters, composed of mass-...

 Laiq Khan1, Sidra Mumtaz2, Kamran Javed3, S.W. Shah4, M.I. Khan Babar5, S.R. Akbar6

GENERATORS MAINTENANCE SCHEDULING USING HARMONY SEARCH ALGORITHM
...great power of this method, HS algorithm is applied to solve GMS problem of different power production systems. Simulation results reveal that the proposed algorithm is a powerful search algorithm for various optimization problems.
...

 Faizullah Mahar1, Syed Saad Azhar Ali1, Abid Karim1

DESIGN OF FIXED ORDER ROBUST CONTROLLER USING H∞ -NORM AND EVOLUTIONARY TECHNIQUES: COMPARISONS AND PERFORMANCE ANALYSIS
...oreover, in these design methods the order of controller cannot be fixed a priori to control law design. In industrial applications it is hard to execute these controllers practically. To overcome this problem, this paper proposes the use of evolutionary techniques (i.e. genetic algorithm and immune algorithm) for the designing of a low, fixed order robust controller. To check the effectiveness of the proposed approach, resulting controller...

Arshad Habib Malik*, Aftab Ahmed Memon** and Muhammad Rafiq Khan***

DYNAMIC MODELING AND CONTROLLER DESIGN FOR NUCLEAR POWER PLANT
...ing fast output sampling methodology. Noise sensitivity is a typical problem with this type of controller. In this research work, a small deviation is allowed in order to reduce the noise sensitivity. The problems of noise sensitivity and poor output error dynamics are addressed and a multi-objective optimization problem is solved using LMI (Linear Matrix Inequalities) formulation. Fast output feedback gains are computed using LMI tool for ...

 Muhammad Shaheen*, Muhammad Shahbaz*, Zahoor ur Rehman* and Sajid Mehmood*

A METHOD OF DATA MINING FOR SUSTAINABLE HYDROCARBON EXPLORATION
...ing upon these values, a methodology is proposed in this paper to find optimal hydrocarbon exploration rate that will ensure sustainable energy development in any country. The sustainability indicators (SI) are classified into exploration SI and consumption SI. The indicators which have strong correlation with hydrocarbon exploration rate are considered to be exploration SI while the indicators which have strong correlation with consumption...

Rafi Javed Qureshi* and Iftikhar Hussain**

INTERNATIONALIZING MANUFACTURING SMEs IN A DEVELOPING REGION: AN EXHAUSTIVE CDA-BASED EXPLORATORY APPROACH
...c is categorical, single methodology to analyze factors influencing exporting are far from sufficient. A multi-method and exhaustive approach is necessary to explore the adequacy of factors in question. The three methods relevant to categorical data analysis (CDA) in this paper highlights the enabling role of the exploratory factors in understanding the relationship with SME...

Muhammad Usama Hameed*, Zulfiqar Ali Gurmani, Sajjad Khan and Allah Bakhsh 

...re made, and head-to-row method was used to reduce hetrozygosity. In 2009-10 and 20010-11, this line was evaluated in preliminary yield trials, where it produced average 80.71 tha-1 green fodder yield while S-2000 produced 67.13 tha-1 (16.82% more green fodder yield than check). In terms of dry matter 20.100% increase in dry matter yield over check variety S-2000 was recorded (PARC Oat: 21.84 tha-1, S-2000 17.45 tha-1). In National Uniform Fodder Yield Trials ...

Zahid Hassan Tarar1, Muhammad Salik Ali Khan2*, Shahzada Munawar Mehdi3, Raza Salim4, Irfan Ahmad Saleem1, Saima Nazar5, Munir David2, Tahir Majeed6, Muhammad Sufyan Mughal7, Muhammad Saleem8, Umer Iqbal9, Muhammad Mazhar Iqbal10 and Muhammad Khalid Shaheen

...cetate (DTPA) extraction methodology. Quantification was carried out by Flame Atomic Absorption Spectroscopy (AAS). Hot water-soluble (HWS) Boron (B) was determined spectrophotometrically. Additionally, soil samples were also analysed for electrical conductivity (EC), soil pH and soil organic matter (SOM) contents. These characteristics were correlated with plant available micronutrients. The DTPA-extractable Zn, Cu, Fe, and Mn ranged from 0.07-5.60, 0.11-1.22...
Magbolah Salem Helal Alzahrani
... using the retro-orbital method to confirm liver damage and estimate liver function. Rats given CCl4 prior to feeding M. chamomilla demonstrated decrease in serum lipoprotein HDL, LDL, VLDL fraction levels, indicating liver damage. Rats given CCl4 and fed on 5% M. chamomilla showed the most significant increase in organ weight as compared to all levels of treatment suggesting that M. chamomilla can reduce liver damage...
Ayesha Noreen1, Amina Elahi1, Dilara Abbas Bukhari2 and Abdul Rehman1*

 

...etric and molybdene blue methods) at pH 7 and 37°C in the presence of Zn2+ as cofactor. The protein profile of B. cereus 3.1S, showed two bands of approximately 14 and 70 kDa, which had their possible role in arsenite oxidation. This was confirmed by transforming E. coli DH5α with plasmid DNA of B. cereus 3.1S. This arsenite resistant bacterial strain oxidized 76 and 86.5% As3+ from the original industrial w...
Jam Nazeer Ahmad1,*, Samina J.N. Ahmad1,2,*, Mubashir Ahmad Malik1, Abid Ali1, Muhammad Ali3, Ejaz Ahmad1, Muhammad Tahir2 and Muhammad Ashraf2
...s extracted through CTAB method and polymerase chain reaction was performed using mitochondrial cytochrome oxidase I (COI) gene based primers. Gel electrophoresis of the PCR products of desert locustsindicated a 710bp amplified DNA fragment on 1.5% agarose gel. DNA sequence analysis of PCR product indicated that specimens shared a 99-100% identity with Schistocerca gregaria gregaria)(Forskål, 1775). Molecular phylogenetic analysis and evolutionary...
He Wenyun, Zhi Yijin and Shao Mingqin*
... we used the point count method to survey the sex ratio, habitat selection and foraging behavior of Mandarin duck (Aix galericulata) in water systems (Raohe River, Ganjiang River and Xiuhe River) of Poyang Lake, Jiangxi Province, China. We recorded 162 individuals and determined 141 by gender: the sex ratio was 1.07. There was no significant male bias (n = 141, p = 0.210). Habitat selection results indicated that Mandarin duck tended to ch...

Tao He1,2,3, Cheng-jing Chen4, Jian-guang Qin3, Yun Li1,2, Rong-hua Wu1,2 and Tian-xiang Gao4,*

...and test a complementary method to quantify the variation of sagittal shapes to discriminate L. haematocheilus stocks. The sagitta variation of five L. haematocheilus stocks was examined using nine shape indices (Roundness, Circularity, Form-factor, Rectangularity, Ellipticity, Radius ratio, Feret ratio, Aspect ratio and Surface density). Multiple comparisons on shape indices showed that there was no significant difference among the Ying K...
Shikun Ge1, 2, Yanli Yang3, Brindha Chelliappan4, 5, Antonysamy Michael5, Fagang Zhong1 and Xiaoying Zhang1, 2, 3, 4*
... However, the extraction methods and storage stability of IgY antibodies from egg yolk for feed supplements remain major problems in mass production. Based on Based on IgY antibody titer, IgY thermostability, IgY acid and alkali resistance, resistance to trypsin and pepsin, four different IgY purification methods (PEG extraction, water dilution method, the effect of liquid egg yolk and egg...
Huma Abbas1*, Nazir Javed1,Muhammad Kamran2, Sajid Aleem Khan1Hira Abbas3, Ehetisham-ul-Haq2, Abdul Jabbar1, Mehwish Naz1 and Ihsan Ullah4
...of different application methods; soil drench and root dip was also tested. Results revealed that all the chemicals have more or less systemic effect against M. incognita. Cartap was found to be more effective in reduction of egg masses followed by Virtako in both treated and untreated root portion. Number of egg masses was reduced significantly in Cure and Azadirachtin in treated (45.7, 58.2) as well untreated root portion (93.7, 80.7) respectively. In...
Yan Wen1, Liu Lian2, Zhuang Xun3, Murong Jiaying4, Cui Shuyi1, He Wanwen1, Mai Guanghuai5, Huang Wenzhu1, Chen Gang1,* and Wang Zhijun1,*
... exercise rehabilitation method.
...
Ishrat Perveen1, Yasar Saleem2 and Javed Iqbal Qazi3* 
...high temperature cooking methods and higher quantities of HCAs in RTE Chicken kabab commercially available from Pakistani markets.
...

Israr Yasin1, Ismail Qureshi1*, Shahjahn Qaimkhani1, Gulam Mustafa Solangi3, Ismail Qaimkhani1, Ismail Brohi1 and Abdul Ahad Soomro2

A Perspective on Household Dairy Farming in District Naushahro Feroze, Sindh, Pakistan
...ng multistage clustering method. Estimates of the study revealed that 54% of the households possessed buffaloes. Average number of buffaloes was estimated at 4.28 while 376 (63%) of the household reported cattle with average number of 4.38 heads per household. Small numbers of buffalo and cattle bulls were recorded. Average number of 5.55 goats per households was reported by 35% of all the households. On an overall basis, 2,365 liters of buffalo milk was compu...

Ummad ud Din Umar1, Syed Atif Hasan Naqvi1*, Ishfaq Ahmed1, Ateeq ur Rehman1, Muhammad Asif Zulfiqar2, Sibghat Ullah3, Shah Pasand4, Zehri Khan3 and Abdur Rehman5

Prevalence and Serological Detection of Spiroplasma citri, a Cause of Citrus Stubborn Disease in Southern Punjab, Pakistan
...ological based detection methods by conducting a comprehensive survey in various districts of Southern Punjab. The results of the survey and serology of CSD showed that this destructive disease which causes symptoms mostly similar to citrus greening is present in all areas of South Punjab. Layyah, and Multan showed maximum incidence of CSD with “11.66” and “5.66%” respectively while least incidence was observed in District Bhakkar (03%)...

Md. Aminul Islam1*, A.N.M. Aminoor Rahman2, Mohammad Shah Alam3 and Md. Taimur Islam4

Gastrointestinal Nematodiasis in Quail in Bangladesh
...analyzed by direct smear methods and nematodes identified by the presence of characteristic eggs in the feces. Five types of nematode eggs found on different farms. The overall prevalence of gastrointestinal nematodiasis were (17.5%), which could consider relatively higher. The highest rate of infection found for Heterakis gallinarum (7%) and the lowest rate of infection found for Trichuris spp. (1.5%). A significant difference in nematodiasis in different far...

Pankaj Kumar Jha1,2*, M. Golam Shahi Alam1 and Farida Yeasmin Bari1

Pregnancy and Lambing Rate Following Laparoscopic Artificial Insemination with Two Different Types of Diluent and Frozen-thawed Sperm Dose in Ewes
...ld be the most practical method for achieving high pregnancy and lambing rate following LAP-AI in Bangladeshi ewes.

...

Tahseen Ullah* and Noor ul Amin

In Vitro Antibacterial Activity of Medicinal Plant Extracts
...oli using disc diffusion method to uncover antibacterial potential of the plants extracts. The antibacterial investigation was performed in three replicates using disc diffusion technique using Nutrient Agar Media for the growth of B. subtilis and E. coli bacteria. The ANOVA for inhibitions zones indicates the effect of plants, bacteria strains, and concentration on growth of bacteria in the bacteria growth culture medium and their interactions. The plants cru...

Shahzad Khan*, Munir Khan, Inayatullah Jan, Mahfooz Khan and Fida Muhammad Khan

Determinants of Sugarcane Yield in District Charsadda, Pakistan
...h face to face interview method from 41 randomly selected sugarcane growers through structured questionnaire. The selection of farmers was based on proportional allocation technique in the selected villages. For data analysis, profit margin, gross margin and Cobb-Douglas production function were applied. The study found that the per acre profit of sugarcane was 289.82 US$. Labour and fertilizers costs were found major components in the total variable cost. The...
Muhammad Yaseen1*, Xu Shiwei2, Yu Wen2, Ejaz Ashraf1, Muhammad Luqman1, Muhammad Ameen3 and Sadia Hassan4
Farmers Behavior to Participate in Public Sector Agricultural Extension Services: Comparative Analysis of Pakistan and China
...cted using the interview method with the help of validated and expert reviewed interview schedule. The results revealed that the household head’s average age is 47 years in Pakistan and 53 years in China. The old aged household head’s participation in extension services in Pakistan is less (36%) than China (43%). Similarly, household head’s education is 9 years schooling in Pakistan accounting less participation (25%) in agricultural extensio...

Muhammad Imran1,2*, Shahbaz Talib Sahi1, Muhammad Atiq1 and Amer Rasul3

Brown Leaf Spot: An Exacerbated Embryonic Disease of Rice: A Review
...he alternate significant method for quick and efficient control of diseases and improving the yield of rice. While, the use of phytoextracts and antagonist are considered to be safe, eco-friendly, cost-effective economically and biodegradable. The use of plant activators as another new strategy that activates the defense system of plants and reduces the disease. The plants which are scarce nutrients are more prone to disease as compared to nutrient deficient. ...

Christian Marco Hadi Nugroho1 and Jeanne Adiwinata Pawitan2,3,4*

Production of Antibody for Direct Fluorescence Antibody Assay against Avian Influenza H9N2
...ies (OIE) suggested some methods for the detection of H9N2 infection including the immunofluorescence method that is commonly known as the direct fluorescent antibody (DFA) assay. This method is a combination of immunology and bio-imaging. This DFA assay is rapid and sensitive, but specificity is influenced by antibody production methods. Therefore, the ...
Sher Bahadar Khan1, Mumtaz Ali Khan2,*, Hameed Ullah Khan3, Sher Ali Khan4, Shah Fahad5, Faheem Ahmad Khan6, Irshad Ahmad7, Nighat Nawaz8, Sidra Bibi9 and Muhammad Muneeb10
...ics using disc diffusion method followed by detection of their respective antimicrobial resistant genes. Overall prevalence of Campylobacter jejuni was 14% being higher in Peshawar division (21%) followed by Bannu division (16%), Malakand division (13%) and Hazara division (8%). Over all highest antibiotic resistance was found against AMX (93%) followed by LIN (88%), AMP (86%), TET (82%), SXT (75), CHL (68%), CLR (65%), STR (50%), GEN (44%), OFX (27%), ...
Muhammad Ilyas1,2*, Imran Khan2*, Muhammad Umer Chattha2, Muhammad Umair Hassan2, Muhammad Zain3, Wajid Farhad4, Sana Ullah1, Ateeq Shah5, Safeer Ahmed6, Bismillah Khan1 and Muhammad Adeel1
Evaluating the Effect of Zinc Application Methods on Growth and Yield of Wheat Cultivars
...f various Zn application methods on the growth and yield of wheat cultivars during the growing season 2014-2015. The study was comprised of three cultivars of wheat; Galaxy-2014, Punjab-2011 and Millat-2011 and Zn application methods; seed priming @ 0.4% Zn solution, soil application @ 40 kg ZnSO4. H2O ha-1 foliar spray @ 0.5% Zn solution at vegetative stage and combination of all three method...

Imtiaz Hussain1, Azhar Mahmood Aulakh2, Muhammad Sohail1*, Khalid Hussain2, Ansaar Ahmed3, Abdul Hamid3 and Muhammad Imtiaz3

Impact of Zero Tillage on Productivity of Traditional Mung Bean-Wheat Cropping System of Punjab, Pakistan
...y. Zero tillage planting method provides an opportunity to improve crop yield, reduce production cost and GHG emission. Farmer participatory field trials were carried out at six locations in Punjab province, a major Mung bean-Wheat cropping zone to evaluate zero tillage based crop production system in comparison to the traditional tillage. The findings revealed that zero tillage produced 13% and 9 % higher wheat and mung bean yield, correspondingly in comparis...
Taqiur Rahman1, Tabassum Yaseen1*, Ali Mujtaba Shah2, Samiullah3, Ghulam Jelani3, Gul Nawaz1,Qudratullah Kalwar2
...xpertise and skills; the methodologies and methods used to treat diseases, maintaining animal health and its importance to ethnobotany and Pharmacogonosy.The objective of the present study is to identify the leading medicinal plants used by local communities for the treatment of animals and document the threatened herbal knowledge from the elder formers (R1, R2 and R4) this study labels the ethno veterinary practices in diff...
Tehreem Zahra1, Muhammad Irfan1, Muhammad Nadeem2, Misbah Ghazanfar1, Qurratulain Ahmad3, Shaukat Ali4, Farzana Siddique5, Zarina Yasmeen6, Marcelo Franco7
...tion by response surface methodology has been investigated. Three variables like substrate concentration, peptone and KH2PO4 were optimized at three levels. The optimum carboxymethylcellulase (CMCase) activity and filter paper cellulase (FPase) activity was obtained after 96 hours of incubation with optimum conditions of media containing 5% substrate concentration, 0.05% peptone and KH2PO concentration of 0.5%...
Zhi Chen1, Toshifumi Minamoto2, Longshan Lin3 and Tianxiang Gao4,*
...e process to employ this method. In eDNA extracting of macro-organisms, DNeasy Blood and Tissue Kit was commonly used, but its price was about 80 RMB/sample, which was too expensive. Cheaper eDNA extraction kit was a potential aspect for improvement to expand further application of eDNA analysis. In order to examine some cheaper kits’ effectiveness of extracting eDNA of macro-organisms and select an optional low-cost kit, a test among three kits was held...
Muhammad Zeeshan Majeed1*, Muhammad Shahzad Akbar1, Muhammad Afzal1, Muhammad Mustaqeem2, Muhammad Luqman3, Ijaz Asghar4 and Muhammada Asam Riaz1
...andard filter paper disc method was used for both toxicity and repellency bioassays according to completely randomized design. The response of termite workers varied with plant species, botanical concentration and exposure time. The extracts of Azadirachta indica (neem) and Nerium indicum (oleander) appeared to be most effective against the termites with minimum LC50 (6.35 and 10.38%, respectively) and LT50 (12.11 and 17.49 ...
Muhammad Zeeshan Majeed1*, Muhammad Afzal1,Muhammad Asam Riaz1,Kanwer Shahzad Ahmed1, Muhammad Luqman2, Mehar Zubair Shehzad1, Muhammad Bilal Tayyab1, Mujahid Tanvir1 and Saadia Wahid1
...ilter paper-dip bioassay methods, respectively. Results revealed that the extracts of Mentha longifolia, Sonchus asper and Nerium indicum were the most toxic to D. citri exhibiting 90% mortality. The extracts of Dodonaea viscosa and Olea ferruginea caused highest mortality of S. litura (i.e. 70 and 58%, respectively). Maximum mortality of C. quinquefasciatus larvae was observed by Maerua ...

Agustine Christela Melviana1, Rizkita Rachmi Esyanti1*, Roy Hendroko Setyobudi2, Maizirwan Mel3, Praptiningsih Gamawati Adinurani4 and Juris Burlakovs5

Gene Expression Related to Steviol Glycoside Synthesis Produced in Stevia rebaudiana (Bert.) Shoot Culture Induced with High Far-Red LED Light in TIS RITA® Bioreactor System
...via plants using in vivo methods, an alternative technique of propagation using in vitro methods is needed. The micropropagation method with the RITA® (Recipient for Automated Temporary Immersion System) is used in the production of a large amount of stevia biomass in approximately a short period. The forming of flowers is one of the limiting factors interfering with the metabolite pro...
Mumtazur Rahman1, Farhan Anwar Khan1,*,Umar Sadique1, Ijaz Ahmad1, Shakoor Ahmad1, Faisal Ahmad1,2, Hayatullah Khan1,3, Muhammad Saeed1, Faiz Ur Rehman1, Ibrar Hussain1, M. Faraz Khan1, M. Izhar ul Haque1 and Hanif-ur-Rehman1,3

...n by broth microdilution method. Quinolones including moxifloxacin, levofloxacin, ciprofloxacin, and enrofloxacin were selected for in vitro susceptibility of Mccp Pakistan strain. The concentration for each drug was measured distantly depending on high concentration available in the market and was then used in various concentrations ranges from higher to lower i.e. moxifloxacin 80-0.156 µg/ml, levofloxacin 250-0.488 µg/ml, ciprofloxacin and...

Zubia Rahim1, Gulnaz Parveen1*, Salma Gul2 and Khushnood-ur-Rehman3

Ameliorating Effects of Salt Stress (KCl, NaCl) on Growth and Germination Parameters of Pearl Millet (Pennisetum americanum)
... Significant Differences method. 100mM concentrations of the said two salt and a mixture of two salts concentrations was less stress causing on all parameters studied. The degree of toxicity of the salts decreases as NaCl<KCl<NaCl+KCl.
...
Yifan Ni1, Jian Liu1, Fen Wu1, Jianfeng Cai1, Jinzhi Zhang1*, Jianqing Hua2 and Jiping Fu2
...ypes, and DNA sequencing method was employed to detect the potential SNPs in this study. There were two SNP loci found in these genes totally. One mutation (G→A) was detected in exon 1 of MDR1 gene, which caused a synonymous mutation of amino acid (Leu→Leu), the other one (T→A) in exon 7 of OPN gene, resulting in a non-synonymous mutation (His→Gln). The association analysis indicated that the GG genotype of MDR1 gene an...
Muhammad Saeed Shah1, Fazal Nasir2, Sana Ul Haq1 and Muhammad Tahir Khan2*
...e into memory cells. The methodology presented in this paper is autonomous and de-centralized. In the proposed system, the robots work effectively to complete a task either alone or co-operatively. Specifically, the coordination and communication among various robots are established based on the concept of immune system, idiotypic network theory and clonal selection theory. The developed methodology is successfully implement...

Imran Ahmad Rana*, Muhammad Ali Yab and Ch. Abdul Rehman

Quality Engineering Tools Implementation a Comparison of Indian and Pakistani Business Organizations
...g and implementing DMAIC methodology. 

...

Muhammad Kashif Khan1*, Muhammad Wasim Jan Khan2, Kaleem Ullah Jan Khan3, Muhammad Ishaq Khan4, Muhammad Faheem Jan Khan5 and Abdul Salam Lodhi6

 

Impacts of Construction Risk Attributes on Organizational Performance of Registered Construction Companies in Khyber Pakhtunkhwa, Pakistan
...tigation through various methods and techniques. The purpose of this study is to address the need of risk management practices in the construction industry of Khyber Pakhtunkhwa (KPK), Pakistan. The research work carried out with the involvement of 150 Pakistan Engineering Council (PEC) registered construction companies not only emphasizes on the crucial risk factors associated with construction projects but also insights into the impacts of the risk factors o...

Mohammad Adil1*, Muhammad A. Khan2, Naveed Ahmed1, Edward Bromhead3, Muhammad A. Arshad1, Muhammad Fahad1, Shahid Ullah1 and Khan Shahzada1

Numerical Investigation of the Effect of Column Staggering in Flat Slabs Systems
...ftware along with manual method of analyses and design called “yield line method” applied to staggered column arrangement. To analyze more patterns in less time, finite element results has been validated with yield line method. Based on the analyses and design results for different case studies, column staggering guidelines have been developed and presented. To prove the concep...

Mehreen Ijaz

Effect of Weave Type on Tensile Strength of Cotton Fabrics
Q. Sun1, Q. Liu1, R. Di1, Y. Wang1, S. Gan2, S. Liu2, X. Wang1, W. Hu1, X. Cao1, Zh. Pan1, X. Guo1, Y. Yang3, H.E. Rushdi4* and M. Chu1*
...RSP gene. Genotyping method was used to analyze genotypes among Altay sheep (fat-rumped breed) and White Suffolk (thin-tailed breed) by Sequenom MassArray. In the c.1031C>T locus, the frequency of T allele of fat-tail breed was significantly (p<0.001) higher than that of the thin-tailed breed using F test, which suggest that this locus may be associated with fat deposition in fat-tail breed. Gene expression levels in both subcutaneous adipose and tai...
Rehman Akhtar1, Khizar Azam2, Abdur Rehman Babar1, Qazi Salman Khalid1, Rashid Nawaz1 and Imran Ahmad1 
 
...ost frequently used MCDM methods in literature, the Analytical Hierarchy Process (AHP) is integrated with fuzzy logic, called as F-AHP, is used in this research to identify and evaluate solar farm location in division of Khyber Pakhtunkhwa (KPK). Thana, Chakdara, Harichand, Palai, Pir Khel and Shergarh city are evaluation by Fuzzy AHP on the basis of above-mentioned factors. Shergarh city is selected for most suitable location for the installation of solar pan...
Arnab Tanveer, Shabana Naz*, Azhar Rafique and Asma Ashraf
...e analysis. Direct smear method and fecal floatation technique was used for isolation and identification of endo-parasites. Modified McMaster technique was used to calculate Eggs per gram. The data was subjected to ANOVA and Tukey-HSD (post hoc test). Six species of endo-parasites were identified from Jallo Wildlife Park. Eimeria (7500) and Ascaris (1100) were the most abundant. Murree Wildlife park had only two species i.e. Eimeria (3850)...

Shawaiz Iqbal1*, Nadeem Iqbal2, Usama Bin Khalid1, Muhammad Usman Saleem1, Adila Iram1, Muhammad Rizwan1, Muhammad Sabar1 and Tahir Hussain Awan1

Growth, Yield and Economic Analysis of Dry-Seeded Basmati Rice
...lign: justify;">Planting method is an important aspect of production technology for the maximum resource utilization by crops per unit area. Dry-seeded rice (DSR) is a contemporary resource saving crop establishing technique, which enables farmers to harvest economical rice production. Field study was conducted at Rice Research Institute, Kala Shah Kaku, Pakistan in 2014 and in 2015 to evaluate the different planting methods...
Wentao Wang1,2, Xu Lin3, Jianshu Zhuo3, Dongjie Zhang2, Xiuqin Yang3* and Di Liu1, 2*
...edicted by bioinformatic methods. The results increase our knowledge of E2F3b mRNA diversity and provide basis for in-depth functional research.
...
Nadia Islam1, Khalil Ahmed1,*, Maisoor Ahmed Nafees1, Mujtaba Khalil2, Ishtiaq Hussain3, Muhammad Ali1 and Raja Imran1
...d by membrane filtration method. At Misto uch, the mean temperature was 9.7°C and 15.4°C, turbidity was 0.44 NTU and 0.67 NTU, electric conductivity was 147.4 µS/cm and 226.7 µS/cm, total dissolved solids was 99 mg/l and 118 mg/l, pH was 6.8 and 6.8 and total phosphorus was 48.3 µgP/L and 64.3 µgP/L in both the seasons. Whereas Bar spring the mean values in both the seasons were 10.8oC and 16.0oC for t...
Minghao Gong1*, Shiliang Pang2, Zhongyan Gao2, Wanyu Wen1, Ling Zhang3, Gang Liu1, Huixin Li1, Fawen Qian4 and Wenfeng Wang2
...lso, organic agriculture methods and crop species that consume less water than rice should be adopted to mitigate the adverse climate changes that accompany global warming and will contribute to RCC habitat degradation.
...
Shikang Deng1,2, Yan Jin2, Jing Xu2, Xiufang Zhu2, Pinghai Hu2, Jiao Li2, Li Zhang2 and Jianzhong Tang2,*
...h of intervention, CCK-8 method was used to detect the cell proliferation level of each group; Western blot was used to detect the expression of TGF-β1, cyclinD1, and CDK4 protein in each group of cells; and real-time polymerase chain reaction was used to detect the expression levels of TGF-β1, cyclinD1, and CDK4 mRNA in each group of cells. The results showed that after 5-FU intervention, the proliferation of bile duct scar fibroblasts was inhibited...
Somia Shehzadi1, Sana Javaid Awan2, Maryam Javed1*, Asif Nadeem1 and Tahir Yaqub3
...sts (ATFs) is a feasible method to study hallmarks of tendinopathy, but ATFs should be viable and grown enough. For this purpose proper medium and supplements are required, which contain suitable growth factors for viability of ATFs. Fetal bovine serum (FBS) is used extensively in cell culture but being expensive and scarce in availability, its alternatives are needed. This study proposed autologous equine serum (AES) and adult bovine serum (ABS) to be evaluat...
Hannan Nasib Hamid1,*, Muhammad Rais1, Muhammad Arif2 and Rubina Noor2
... visual encounter survey method aided with pitfall and funnel trapping. Twenty three herpetofauna species (14 recorded through direct sightings) were recorded which included two species of amphibians and 21 of reptiles (eight snakes, 13 lizards). Species in the sub-tropical broadleaved evergreen forest were more diverse with Common Leopard Gecko, Persian Leaf-toed Gecko, Reticulate Plump-bodied Gecko as notable species while tropical thorn forest had species s...
Jun Yan Bai*, Kun Peng Shi, Xiao Ning Lu, Xiao Hong Wu, Xue Yan Fu, Heng Cao, Hong Deng Fan, Meng Ke Chen and Yong Kang Ma
...was detected by PCR-SSCP method in this study. Moreover, correlations of control regions of MyoG 5’ with slaughter performance of quail were analyzed. Results demonstrated that: In meat quail, three genotypes (AA, BB and AB) were detected at locus A in the control region of MyoG 5’. For locus A, BB frequency of French giant quail and Savimit quail was the highest (0.531 and 0.750). For locus B, three genotypes (AA, AB and BB) were det...
Alam Zeb* and Amanullah Jan
...onse of sesame to sowing methods, nitrogen and sulphur levels” were carried out in Agronomy Research Farms, The University of Agriculture, Peshawar. During two consecutive Kharif growing seasons of 2013 and 2014, respectively. Three sowing methods (flat, ridges and raised bed) were kept as main plot factor, whereas four nitrogen levels (00, 60, 120, and 180 kg ha-1) and four treatments of sulphur (...

Hillary M.O. Otieno

A Review of White Mango Scale (Aulacaspis tubercularis Newstead; Hemiptera: Diaspididae) in Sub-Saharan Africa: Distribution, Impact and Management Strategies
...tive traditional control methods. Farmers capable of using pesticides apply very toxic products that are not only harmful to the applicators but also to the beneficial organisms. Agronomic management strategies like right varieties, spacing, better fertilization and shed management are also applied inappropriately. This review is therefore essential in providing the basis of identification of A. tubercularis. It also provides management strategies that could b...
Muhammad Umar1, Muhammad Arshad2, Mubashar Hussain1*, Moazama Batool3 and Muhammad Faheem Malik1
Nasir Rashid1,2*, Javaid Iqbal1,2, Umar Shahbaz Khan1,2, Mohsin Islam Tiwana1,2 and Amir Hamza1,2
...e subjects. The proposed method has shown superior experimental results as compared to the previous studies using the same dataset for calculating multi-class classification accuracy. 
...
Tariq Mahmood Khalil1,2*, Muhammad Ajmal3, Iftikhar Zeb1,4 and Muhammad Azeem Khan1,5
 
 
...ured soils using a novel method based on water potential dynamics. Chilled mirror dew point hygrometer accurately captured the water potential dynamics when soil samples were irrigated with saline water. The instrument output showed that soil texture has profound effect on soil total water potential rising the water potential up to −4.56 for coarse soil and −9.11 MPa for fine textured soil. The instrument recorded a slow salt buildup in the coarse ...
Shahid Hussain Abro1*, Mohammed N. Alghamdi2, Muhammad Sohail Hanif3, Hazim Moria2 andHamza Suharwardi1
 
 
...°C has been examined methodically. The soaking time and holding temp: play an important role in developing of gamma-austenite. Mostly ferrite and cementite morphology exist at nucleation sites. At ferrite-ferrite grain interface the austenitic nucleation is not found. At high temperatures, the development of austenite at pearlitic nearby areas are highly noticed. At 800°C growth is about finger-type morphology. The fingers are parallel to pearlite so i...

 Shuanping Zhao, Lei Xu, Hai Jin and Yutang Jia*

...ng and (forced) PCR-RFLP methods, three polymorphisms (g.1500G>A, g.1694T>A and g.2213C>G) were identified and genotyped in our population (n=298). Genetic diversity analysis showed that g.1500G>A and g.1694T>A belonged to an intermediate level of genetic diversity (0.25<PIC<0.5), and SNP g.2213C>G belonged to a low polymorphism level (PIC<0.25). LD (Linkage disequilibrium) analysis showed that SNP g.1694T>A and g.2213C>G had a...
Huan Yang1, Tingting Li1, Huimin Zhao1,2, Xiaoyuan Zhang1, Huiyan Xu1, Yangqing Lu1, Xingwei Liang1, Shengsheng Lu1, Xiaogan Yang1*and Kehuan Lu1*
...lo SSCs in vitro, methods for their isolation, enrichment, culture and preliminary identification were established. Testes from 3- to 6-month-old buffalos were digested with two-step enzymatic treatment to obtain isolated single cells and then enriched with a differential plating method. Isolated SSCs were cultured on STO feeder cells with SSCs culture medium (SSCM), which is serum-free. SSCs began to proliferate on t...
Faheem Ahmed Khan1*, Sarzamin Khan2, Qurat-ul-Ain3, Saqib Ishaq1Muhammad Salman1, Abdul Rehman1, Ikram Ullah4, Kalsoom5 and Johar Jamil5
...ary to explore alternate methods for production of non-conventional proteins utilizing the available cheap sources. The indigenous Saccharomyces cerevisiae were isolated from different fruit samples, identified by conventional polymerase chain reaction (PCR) and were characterized for production of single cell microbial protein (SCMP). Out of 60 different fruit samples, a total number of confirmed S. cerevisiae isolates were 1, 2 and 1 from orang...
Amjad Ali, Kafeel Ahmad*and Shaista Rahat
...rby Bauer disc diffusion method using nineteen different antibiotics and multiple antibiotic resistance (MAR) index was determined. Prevalence of extended spectrum β-lactamases was studied by double disc synergy test. The ESBL genes blaCTX-M, blaOXA-10, blaPER-1, blaSHV and blaTEM were analyzed by PCR amplification among the isolates. Susceptibility to antibiotics was: imipenem (85.02%), meropenem (82.88%), cefepime (76.47%), piperacillin-tazobactam (76.4...
Małgorzata Dzierzęcka1, Sławomir Paśko2, Marcin Komosa3, Karolina Barszcz1, Bartłomiej Jan Bartyzel1 and Ewa Czerniawska-Piątkowska4*
...m the measurements. This method enabled the first objective assessment of the distribution of angles between the coffin bone parietal surface and the ground, which had not been possible using other imaging methods. The angle between the coffin bone parietal surface and the ground was found to be correlated with age, with a 0.27 correlation coefficient at p=0.1, but not with body weight. The correlation coefficient for body w...
Mashal Malik and Mudassar Nawaz Khan*
 
... SAS by Neighbor-Joining method which clustered the soybean lines into two groups. Maximum genetic dissimilarity (7) was found between line B24G14 and line B23G16. From the results of the study, it was clear that B20G16 had maximum morphological growth, while B29G11 revealed minimum growth among the tested lines, although these two lines were clustered into the same cluster interestingly. It can be concluded that line B20G16 may be further analyzed and might b...
Kamal Al-Samawi1, Mohamed Al-Hassan2, Hussein Migdadi3,4*, Megahed Ammar5 and Salem Alghamdi3
...23 post NM. These methods will provide early and precise detection of pregnancy compared to the routinely used serum P4 and US methods.
...
Muhammad Qayash Khan1,2, Muhammad Zubair Anjum2, Muhammad Adnan3, Abbas Khan1, Hafsa Zahid1, Javed Nawab4, Sher Zaman Safi5Muhammad Ishaq Ali Shah6, Atif Kamil7 and Abid Ali1*
...he traditional taxonomic methods.
...
Iram Liaqat1*, Tahir Hussain1,2, Aisha Waheed Qurashi3, Gulbeena Saleem2Asia Bibi4, Muhammad Fiaz Qamar5,ShaukatAli1 and Ikram-ul-Haq6 
...icken by three different methods i.e., congo red, test tube and air liquid interface coverslip, (2) biofilm quantification at different time intervals and (3) monitor antibiofilm effect of three proteolytic enzymes including trypsin, chymotrypsin and proteinase k against S. Gallinarum. We observed that S. Gallinarum has strong biofilm forming ability as observed by dark black colonies on congo red medium. Quantification assays such as test...
Riffat Sultana1*, Nuzhat Soomro2, Santosh Kumar3 and Ahmed Ali Samajo2
...tified as a pest control method in which the insect does not become resistant.
...
Tanveer Hussain, Masroor Ellahi Babar*, Akhtar Ali and Fiaz Hussain
...ing the Neighbor Joining method with a bootstrap value of 1000. Finally, the dromedary camel sequences from Pakistan were compared with corresponding genetic sequences of other animal species available on NCBI including European bison, Indian bison, Tibetan antelope, wild yak, domestic goat, sheep, water buffalo and cattle for biological positioning of dromedary camel. The results reconfirmed the classical biological classification.
...
Eman Khalifa1*, Riad Khalil2 and Haitham Elaadli3
...robial sensitivity plate method. A total number of 100 samples of mastitic milk from native lactating buffaloes’ breed were collected aseptically from 4 private farms in Cairo-Alexandria desert road in the north part of the Egypt and subjected to bacteriological and molecular examination for M. bovis followed by their antibiogram profile. Seven (7%) isolates were identified as M. bovis from totally examined 100 milk samplesand isolated M...
Rahmat Ullah Khan, Asif Sadam and Sajid Mahmood*
...0.04/ha by line transect method. During the period June, July, September and October 2017, significantly (P<0.05) high population density was recorded as compared to the population density recorded during May, August, November and December 2017and January-April 2018 both in the morning and evening. Significantly (P<0.05) high population density (0.0060±0.002/ha) was recorded during the autumn season. Different ecological factors...
Saba Shabeer1, Riffat Tahira2 and Atif Jamal3*
 
...on in storage. Different methods including chemical, cultural, and biological control strategies are employed to control this fungus. In this review, mycotoxin production, characterization, identification, and different economically important diseases associated with Fusarium species as well as their control are discussed in detail.
...
Kartika Noerwijati1*, Sholihin Sholihin1, Tinuk Sri Wahyuni1, Rohmad Budiono2, Nguyen Van Minh3, Roy Hendroko Setyobudi4, Zane Vincēviča-Gaile5 and Lulu Husna6
 
 
...K-means cluster analysis method) was carried out to classify clones based on tuber yield and harvest index and to facilitate the selection process. The aim of this study wasto select high yielding cassava clones as selection material for the next stage of selection in the highlands. The F1 seedlings population selected, had a wide range of tuber yield. The results of this study indicated that the average of tuber yield of all families were lower tha...
Salman Ali1*, Ayaz Ali2, Riaz Ali Rind2, Majid Ali3, Zulfiqar Ali Mastoi2, Shagufta Naz3, Muhammad Shakir3, Rashid Ahmed Qaim Khani1
...aled that the cooking in methods were significantly varied (LSD<0.05) for different parameters. The fish cooked in microwave resulted average value of moisture 65.03%, titratable acidity 1.78%, pH value 6.26, protein 24.07%, fat 3.76%. Whereas, the fried fish resulted moisture 62.16%, titratable acidity 1.39%, pH value 5.96, protein 21.11%, fat 5.93%. The fish grilled resulted in moisture 60.19%, titratable acidity 1.32%, pH value 5.89, protein 20.28%, and ...
Tariq Ali Mastoi1, Zulfiqar Ali Mastoi2*, Zahoor Ahmed Khetran3, Ghulam Hussain Alizai2, Binish Baig4, Mitha Khan2 and Syed Jahangir Shah1
 
 
...lochistan through survey method. Eight districts were selected purposively by using random sampling techniques to investigate eligibility, education and obstacles of the respondents for obtaining micro finance loan from Zarai Taraqiati Bank Limited (ZTBL). Out of selected districts; ten respondents were chosen randomly who obtained micro finance loans. The information of the selected districts and respondents was obtained (ZTBL). Thus, the respondents were int...
Shakeel Ahmed Soomro1,2, Sikandar Almani3, Lal Bux4, Khalil Ahmed Ibupoto2, Muhammad Sohail Memon2, Farman Ali Chandio2, Shakeel Hussain Chattha2, Zaheer Ahmed Khan2, Zubair Ahmed Soomro5,Babar Mustafa Ansari6 and Kunjie Chen1*
...y using response surface methodology. A mathematical model was used predicting the effects of indicators i.e. temperature (40.86, 45, 55, 65 and 69.14 ºC) and time (4.11, 30, 92.5, 155 and 180 min) each with five levels on observed parameters. The suitability of quadratic model for present study was described by the responses having acceptable R2 and Adj. R2 values, non-significance of lack of fit which compares residual to pure erro...
Muhammad Nawaz Kandhro1*, Nadia Mangrio1, Aijaz Ahmed Soomro1, Zia-ul-Hassan Shah2, Ghulam Sughra Mangrio3, Nihaluddin Mari4, Zulfiqar Ali Abbasi1 and Shazia Parveen Tunio5
 
 
...ion of suitable planting methods and NPK level play a major role in improving sugarcane yield and quality. Experiment was performed under field conditions at Sugarcane Research Institute, Tandojam, Sindh, Pakistan (located at 250 25.19 N 680 32.07 E) during 2016-17, repeated in 2017-18 and data was averaged. The sugarcane variety PSTJ-41 was used for this study. The experiment was laid out in spilt plot design. Main-plot consisted of planting
Kamran Baksh Soomro1*, Sina Alaghmand2, Sanyogita Andriyas3, Muhammad Rehmatullah Khan4, Naveedullah5 and Amin Talei6
 
 
Hafiz Tassawar Abbas1*, Tamoor Khan1, Ghulam Khaliq2, Muhammad Aqeel Sarwar3, Muhammad Rashid4, Intazar Ali5, Muhammad Abuzar Jaffar2, Ghulam Ali Bugti6 and Muhammad Waseem4
 

...ological and biochemical methods of the host plants which help to prevent the extent of pathogen. 

...
Asma Chaudhary1, Qurat-ul-Ain Ahmad1, Afia Muhammad Akram1, Sadia Roshan2 and Javed Iqbal Qazi3*
...s well as disk diffusion methods with maximum growth inhibition zones i.e., 15 and 17 mm by Sphingomonas sp. AsCh-P3 and Bacillus aerius AsCh-A7 respectively. Six probiotic bacterial isolates of known enumeration (C.F.U.ml-1) showed growth antagonism for the fish pathogen with inocula comprising of less number of C.F.U.ml-1. Highest survival rates i-e 63.5 and 70.17% were expressed by Sphingomonas sp.AsC...
BamideleAkinsanya1, Isaac O Ayanda2,*, Benson Onwusa1 and Joseph K Saliu1
... done using conventional method. Only one parasite species, Teneuisentis niloticus, an acanthocephalan was recovered. There was 72% infection prevalence. The result also revealed that both BTEX and PAHs were accumulated in the body of the parasite. Two components of BTEX, (1,2-dichlorobenzene and 1,3-dichlorobenzene) and five PAH congeners-Acenaphthylene, Phenanthrene, benz(a)anthracene, benzo(a)pyrene and 7,12-dimethylbenz(a)anthracene were not detecte...
Suwati Suwati1, ErniRomansyah1,*, Syarifudin Syarifudin1, Yahya Jani2, Agus Heri Purnomo3
Damat Damat4 and Erkata Yandri5,6
...a-carotene. Right drying methods are needed to preserve the quality of dried seaweed. This study aims to compare the trend of weight reduction in seaweed during drying by natural and cabinet methods. The methods were used experimentally in the field and laboratory. And the data were analyzed using simple linear regression to formulate a trend of reduction in the weight of seaweed while dry...
Titin Herawati1,*, Indra Adiwiguna1, Ayi Yustiati1, Iis Rostini1, Roy Hendroko Setyobudi2, Hafizan Juahir3 and Asep Sahidin1
 

...rvoir. Field observation method and census sampling technique were applied. Research commenced from November 2017 to January 2018. Parameters measured are diversity index, dominance index, equitability index, and water quality. Data analysis employed comparative-descriptive method by mapping the species and comparing the diversity of fish living in both ends of Jatigede Dam. The results indicate that six families of fish are...

Anwar Ali1,5*, Horia Shaukat2, Munir Ahmed3,5, Ahmed Bostani4 and Syed Ashiq Hussain3

...quately along with other methods. In spite of a wide exploration and study on electrical stimulation, the fact that it has been explored and investigated thoroughly in a number of countries around the world. It is currently applied properly on commercial scale in few countries. It has different impact on different type of muscles, so basic know how of changes occurring when it is applied to different kind of muscles should be known to get maximum benefits from...
Shehzadi Saima1, Maqsoda Perveen2, Muhammad Nawaz1, Khalid M. Ahmad1,* and Haider Sultan1
...s by different treatment methods, and to find out which method is more promising. The corms of Gladiolus hybrid cv. yellow stone was used for plantation. Experiment was carried out in complete randomized design (CRD) with four replications and three fertilizer treatments (control, 2g/pot with side dressing, 2g/pot with foliar spray). Results revealed that foliar spray of potassium enhanced most of parameters i.e. leng...

Masood Sadiq Butt1, Sadia Aslam1,2, Rizwan Shukat1,*, Syed Qamar Abbas1, Muhammad Issa Khan1, Shadab Shaukat3 and Muhammad Shahid4

...ed that response surface methodology was employed to optimize the reaction conditions (temperature, time, pH and enzyme to substrate ratio) for enzymatic hydrolysis of fish proteins. The optimum reaction conditions for degree of hydrolysis (DH) using flavorzyme enzyme were temperature 46.37oC, time of hydrolysis 116.91 min, [E]/[S] ratio 1.97% and pH of 7.99. The experimental value of DH at optimized conditions was 50.13% while the statistically pre...
Arshad Bhat1*, Masudul H. Wani1, Shabir A. Wani2, Abid Qadir3, Iqra Qureshi4 and Abid Sultan2
 

Aamer Sattar1, Sadia Sultana1*, Abid Niaz1, Muhammad Aftab1, Ghulam Sarwar2, Irfan Rasheed3, Muhammad Shoaib4, Raheela Naz1, Amina Kalsom1, Nisa Mukhtar1, Farah Rasheed1, Arfan ul Haq1, Munazza Rafique5, Muhammad Arif1, Sarfraz Hussain1 and Jafar Salim6

...rice with 3 major plots (methods of residues management) i. traditional practice by complete removing (C) whole crop biomass from field, ii. burnt (B) practice by burning the crop biomass in field after threshing and iii. Residue’s (R) incorporation practice by rotavating the crop biomass into the soil. The selected field was sufficient in exchangeable K and available P but was deficient in organic matter contents. The results showed that in first year t...

Syed Mazuan, Syed Mohamed, Insyirah Ishak, Dzolkhifli Omar and Norhayu Asib*

... plana. Various spraying methods had been developed for controlling the bagworms. A ground study was conducted in FELDA Gunung Besout 04, Perak, with its main focus on mistblower application towards bagworms. Stihl SR420 mistblower was characterised by having 1.2L / min flow rate, produced volume median diameter of 80μm, and achieved spraying productivity of 2.58 hectares’ land size (approximately 350 oil palms) per man day. The study was to evaluate ...

Muhammad Arslan Ibrahim1*, Aneeqa Aleem2, Farkhanda Manzoor2, Shahbaz Ahmad1, Hafiz Muhammad Zahid Anwar1, Talia Aroob2 and Mansoor Ahmad3 

...e instar. The single-sex method was adopted in the construction of the life table. The ratio of mortality in the late instars (3rd to 5th) was greater than the early instars because of entomopathogenic treatment. The survivorship curve explicated that the 4th to 6th instar larvae, being more voracious, could be destructive for the maize crop if not managed properly. A large number of survivors could enhance the number of individuals in the next generation.&nbs...
Aneeqa Saleem1, Muhammad Islam1, Hamid Saeed1* and Mehwish Iqtedar2
...ained using hot and cold methods. Ethylene glycol (0.75% V/V) was used IP for 10 days to develop mice model of nephrolithiasis. Extracts were administered at a dose of 400 µg/g for 11 – 21 days. Outcome measures included serum creatinine, uric acid and BUN levels. Significant differences exist with regards to primary and secondary metabolites between both peel and pulp extractives. After 21 days of treatment in mice, only chloroform (CHL) extractiv...
Li Shengqing1,2,3, Zhang Xiyun2,3, Liu Shengcai2,3, Hu Guoyuan2, Fan Yuxia2,Liu Huaixin2, Wang Tingting2 and Zhang Yanming1*
...ted through Horn’s method and the improved Karber method. The ability of the toxin to prevent and control rodent damage is assessed through plot experiments. The safety of type D botulinum toxin toward nontarget animals, such as yaks, Tibetan sheep, dogs, vultures, and birds, is also tested. Results show that the toxin is encoded by a type D botulinum neurotoxin gene. The DNA and amino acid sequences of the full neurot...

Hira Soofi, Abdul Rasool Abbasi and Arifa Bhutto

...process through standard method of temporary slide. A new species, Contracaecum malhi, were identifed in having differential diagnostic characters such as body shape and size, shape of cephalic end and its structures, ventricular shape, spicule shape and size, numbers and arrangement of caudal papillae, posterior end with 3 rectal gland and large spine at tip, genital structure of female. The name of new species Contracaecum malhi refers to the name of host wa...
Fuhua Zhang, Yishuang Yu and Shibao Wu*
...mbryos and fetuses. As a method of pregnancy diagnosis, B-ultrasound is not only easy to operate, safe, and noninvasive, but also very intuitive and efficient compared with hormone detection methods. Efficient detection methods such as B-ultrasonography are critical to avoid housing already-pregnant pangolins together with males, which may mate with the females again or stress them, result...

Aioub Sofizadeh1, Ehsan Allah Kalteh2, Shahin Saeedi3, Mulood Mohammadi Bavani4*

...rolling and ultra violet methods from May to September, 2019. Then, the specimens were put into a 75% alcohol-containing plastic bottle. Finally, the specimens were identified using a valid identification key. Distribution maps were prepared using ArcGIS (Ver 10.4.). A total of 111 scorpion samples were captured. All the samples belonged to the Buthidae family, including Mesobuthus eupeus (97.3%), Orthochirus farzanpayi (0.9%), and Mesobuthus caucasicus (1.8%)...

Muhammad Adnan Islam1*, Zia-Ul-Haq2, Rana Shahzad Noor2, Matiullah Khan4, Muhammad Mohsin Ali1, Zulfiqar Ali3, Asif Ali Mirani3, Hafiz Sultan Mahmood1, Muzammil Husain1 and Badar Munir Khan Niazi1

...ed to wheat by broadcast method before sowing crop. This is wasteful method of fertilizer application as only 15-20% of applied phosphate is utilized by the crop. To overcome the problems of phosphorus fixation, fertilizer band placement drill (FBPD) for wheat had been developed and its performance was evaluated at farmer’s fields. This drill places DAP fertilizer near the seed that is about 5 cm away and 5 cm deeper t...
Mohammed  Akinlabi Rufai1*, Abideen Akinkunmi Wahab2, Kamil Ayo Fasasi1, Quadri Olusegun Adeshina1 and Mary Tolulope Awotidebe1
...ation and centrifugation method of nematodes extraction. The five different concentrations of H. bacteriophora were made in distilled water (µg/l); one sample not treated with nematode was served as control. Biocidal efficacy was achieved by collecting soil samples, sterilized and kept into experimental plastic plates labelled as A, B, C, D, and E. This was further divided by weight into five equal portions labelled as A1-A5, B1-B5, C1-C5, D1-D5, and E1-...
Muhammad Furqan1*, Zulfiqar Ali1, Usman Ali2, Muhammad Mudassar Shahzad3, Bushra Nisar Khan1, Waqar Ahmed1, Zakir Hussain1, Mian Amman Ullah1Nimra Afzal1 and Anjum Ara2
...structured questionnaire method. A total of 490 poultry and pets were killed by mongoose during the study period, summing up to an estimated cost of 0.126 million PKRs (784 US$). The highest (n=56) depredation was recorded at Naugran and Mehmoodabad locality and the lowest (n=14) at Rata and Thara localities. Most (66%) attacks were observed in summer season; the preferred (32%) attack timing was the morning time. Three retaliatory killings were reported, 2 at...

Imtiaz Ahmed*, Naveed Ahmed, Abdul Waheed, Muhammad Abbass Khan, Noorullah Khan and Fayyaz Ahmed

...“Impact of growing methods and direction of sowing on the growth and seed production of ridge guard (Luffa acutangula Roxb)” was conducted at PARC-National Tea and High Value Crops Research Institute, Shinkiari, Mansehra, Pakistan during the months of April-September, 2019. The experiment was laid out in Randomized Complete Block design (RCBD) with split plot arrangement having three replications. There were 45 plants in each replication. The treat...

Taqi Raza1*, Kashif Nazir Qureshi2, Shakeel Imran2, Neal Samuel Eash3 and Imma Bortone4

...re time with a treatment method that removes Pb and Fe within standard limits else the discharge will pose pollution and health risks to human beings.

...

Allah Bakhsh1, Attiq Akhtar1*, Fiaz Hussain1 and Shabbir Ahmed2

...rot to less than 5%.This method is extremely useful for maintaining grape quality and quantity without the use of chemicals to combat disease. As a result, this strategy is both environmentally and health-friendly. In Pakistan, we consider using leaf removal therapy to treat grape bunch rot disease because it is the least expensive.

...

Muhammad Rafique1, Inam Ul Haq1*, Humara Umar2, Muhammad Jan2 and Muhammad Azhar Iqbal2

...25 and 30 seconds by dip method on rooting ability of Gemlik cuttings. The data recorded showed that T6 (30 seconds) produced best results for rooting parameters including rooting percent (35.33%), average number of roots (8.36), average root length (9.40 cm) and survival rate of olive cuttings (39.00%). While poor rooting was observed under T1 (5 seconds) with respect to rooting ratio (19.66%), average number of roots (1.96), average root length (3.66 cm) and...

Muhammad Waqas1*, Muhammad Shoaib2, Muhammad Saifullah1, Adila Naseem4, Sarfraz Hashim1, Farrukh Ehsan1, Irfan Ali3 and Alamgir Khan1

...idered as an alternative method for streamflow forecasting.

...

Muhammad Yamin1*, Usama Ali Zafar1, Ghulam Usman2, Muhammad Nauman1, Muhammad Rizwan3, Muhammad Nadeem1, Muhammad Azhar Ali1 and Usman Haider1

...ture sensor and oven dry method. It was explored that an increase in temperature through an air heater decreases the RH in tunnel 1 and 2 compared to tunnel 3 which has higher RH because it was not installed with air heater and exhaust fan. System controlled the temperature and soil moisture within adjusted limits of 22-27 °C, 17-23 % for tunnel 1 and 19-22 °C, and 19-23 % for tunnel 2.

...

Fazal Subhan1,2, Abdul Malik1, Zia Ul Haq1 and Tariq Mahmood Khalil1,3*

...l irrigation is the best method as compared to other furrow irrigation techniques to enhance WUE and to save water without a significant reduction in the yield of cauliflower.

...

Muhammad Ibrahim Kubar1, Fahad Nazir Khoso1*, Imran Khatri1, Niaz Hussain Khuhro2 and Arfan Ahmed Gilal1

...luate the most effective method of pest control. The field experiments in bottle gourd, sponge gourd, Indian squash and bitter gourd were designed in Randomized Complete Block Design (RCBD) having 16 treatments with 5 replications each. Basically, there were five treatments; T1= Untreated control, T2 = Spinosad (Tracer), T3= Cue-lure, T4= Protein hydrolysate, T5 = Trybliographa daci. Furthermore, combinations of these treatments were designed to find out the m...

Weenghar Ali Chandio1, Tajnees Pirzada1*, Abdul Majid2 and Farzana Rashid3 

... Humic Substance Society method and confirmed by UV-Visible spectroscopy and characterized by spectral features obtained by using different techniques such as X-ray diffraction (XRD), Fourier transform infrared (FTIR), and scanning electron microscopy (SEM). Finally, the effect of extracted humic acid on wheat seed germination profile including root/shoot length, germination and fresh/ dry weight was investigated by applying varied concentrations of HA (0, 5, ...

Amar Iqbal Saqib, Khalil Ahmed*, Muhammad Khalid Bhatti, Ghulam Qadir, Muhammad Qaisar Nawaz, Muhammad Ashfaq Anjum, Abdul Rasul Naseem, Aftab Ahmad Sheikh and Belqees Akhter

...acticable and economical methods in two different textured soils. Treatments were: (A) Types of soils. 1) sandy loam, 2) clay loam, (B) Remedial strategies, 1). canal water, 2) saline-sodic tube well water (continuous), 3) three irrigations of tube well water and two of canal water in a cyclic manner (short cyclic use), 4) tube well water + gypsum @ RSC of water, 5) tube well water+ canal water in 1:1 ratio (conjunctive use). Data showed that the highest bioma...

Arshad Khan1, Mohammad Ihsan1, Mohammad Nisar1, Ali Hazrat1*, Murad Ali3, Rashid Ul-Haq3, Khalid Khan2, Karishma Gul1 and Shah Faisal1 

...igated through different methods such as morphological traits, biochemical (SDS PAGE) and molecular markers. The present work was conducted on the basis of morphological and biochemical characterization in order to estimate the genetic diversity among the barley landraces; to explore significant variation which can be used in breeding programs. For this purpose, 40 barley landraces were collected from Dir Lower and Swat, districts of KP, Pakistan. A total of 1...

Samra Aftab1, Saleem Ullah1 and Farida Anjum2*

...by using serial dilution method and were selected on efficiency basis (screening of microbes on the basis of their activities as cellulose degrading agents). The cellulose and related constituent of saw dust and sugarcane bagasse showed that cellulose (49 %), hemicelluloses (23.5 %) and ash (1.8 %) were higher in sugarcane bagasse, while lignin (7.9 %) was higher in saw dust. The data regarding microbe isolation showed highest colonies of Aspergillus sp. (8 CF...
Sana Ilyas1, Muhammad Hidayat Rasool1,*, Muhammad Javed Arshed2, Muhammad Usman Qamar1 and Bilal Aslam1
...ed by the broth dilution method. Phenotypically confirmed ESBL producing strains were further subjected to molecular characterization for the presence of ESBL and carbapenemase-producing genes using PCR. Of 392 samples, 219 ESBL positive E. coli were recovered and among these 156/213 (73.2%), 42/63 (66.6%) and 21/27 (77.7%) were from poultry, environmental water, and urine samples, respectively. The PCR results revealed that71.2% of blaCTX-M...

Iftikhar Jan* and Sahib Alam

...ction) and immunological methods i.e., enzyme linked immunosorbent assay (ELISA). All the four cultural methods successfully differentiated aflatoxigenic and nonaflatoxigenic isolates, however ammonium hydroxide vapor induced test was found to be the most efficient (80.29%) for segregation of the isolates. Among all isolates, thirty were screened for their total aflatoxin production in corn meal agar medium by using ELISA te...

 Ramadan, F. Abbas1;H.M.Salem1; M.H. Belal2, and A. M. M. Abd-Alla3*

...y population using a new method
based on homogenizing large number of house fly adults collected from
field, clarify and concentrate the filtrate by centrifugation with 1400 g
and 28000 g respectively, resuspend the pellet in Tris buffer, sterile by
0.45-μm syringe filter and inject to house fly adults maintained in
laboratory colony, leads to the detection and isolatio...

El-Habbaa, A.S.

...ere
suitable methods for CPDV detection but PCR was most specific and sensitive
diagnostic tool for rapid detection of CPDV.
...
El-Dougdoug K.A.1, Sofy A.R.2, Rehab A. Dawoud3 and Korkar H.M.4
...plant materials is a new method for virus
eliminationbased on osmopreservation techniques. Osmotherapy wasapplied for
eliminationbanana bunchy top virus(BBTV) through preservation of shoot tips on
MS media containing high sucrose concentration at low temperature. Shoot tips and
subculture 1st of banana plantlets were preservedsuccessfully for 12 months on
storage MS medium supplemented with 40, 50, 60...
Mansour, L. L.*; Othman, B. A. **; Abd-EL Ghaffar, M. H. **; Eman, M. Marai** and Sahar, A. Youssef* 
...ed using minipreperation method. Component-6 was sequenced to
determine the phylogenetic with other published isolates. Phylogenetic tree showed
the identity percentage of Egyptian isolate group of BBTV-component- 6 with
component 6 of Indian, Taiwanese and Pakistani isolates group was 97%, whereas
it was 84% in case ofChinese isolate group.
...

Mai A. Kilany1; Hanaa H.A. Gomaa1; Yousry E. Abo El-magd2 and Nermin Raafat2.

... polymorphism (PCR-RFLP) method. The homozygous Ala/Ala (CC) was significantly more frequent in HCV-related HCC patients com-pared to HCV-infected patients and control group (67.5%, 12%, and 15% respective-ly, p ˂ 0.05). Moreover, the C allele was more often associated with HCV-related HCC patients compared to HCV-infected patients and control group (50%, 10%, and 15% respectively, p ˂ 0.05). MnSOD activity was significantly higher in HCV-related HCC patient...
RehamM. El-Bakrey1, Shimaa M.G. Mansour2, Mohammed A. El Sisi1 AndAmal A.M. Eid1
...cination is an effective method for controlling avian influenza virus infections.This study was conducted to evaluate the efficacy of the commercially applied AI vaccine regimens against challenge with three H5N1 HPAI viruses isolated from chickens, Egypt during 2009-2011. Both inactivated and recombinant fowlpox virus expressing H5 (rFP-AI-H5) vaccines were measured. Vaccine efficacy was evaluated by percentage of protection from mortality, morbidity and redu...

Yasser F. Elnaker 1, Mohamed El-Tholoth 2, Sahar Saber 3, Amira A- Elsaid4, Mohamed A. Saad 3, Emad E. Younis 5

...on is the only effective method to control the disease in endemic countries. In the present study, 10 out of 25 clinically examined calves (40%) in a farm in Dakahlia Governorate, Egypt during summer 2013 showed clinical signs suspected to be lumpy skin disease, despite vaccination of dams 4 months before parturition with sheep poxvirus vaccine (Kenyan strain). The diseased calves showed localized or generalized skin nodules with or without fever. Skin lesions...

Ebtisam, A. Abouel yazeed1; Yanni, M.I.1 and Hanan A. Fahmy2

...AT) as well as molecular method as real-time quantitative reverse transcriptase PCR (RT-qPCR) from samples collected during neonatal diarrhea. Six out of forty tested samples were found positive for rotavirus (15%) infection using ELISA test. Successful isolation of the rotavirus on MDBK cell line from two samples from the positive ELISA samples. The beginning of characteristic cytopathic effects (CPE) were observed from the 3rd passages as clumping, rounding ...

Hanaa A. Ahmed1 and Eman M. AboHatab2

...red a rapid and accurate method for LSD antigen detection. nodules were subjected directly to PCR and Real time PCR for rapid and specific detection of LSDV, sixteen out of the twenty one nodular samples were confirmed positive by molecular methods. Eleven out of twenty one nodular samples were positive by (IFAT) in CAM, which prove that PCR and Real Time PCR are much sensitive and rapid diagnostic tool of LSD reflecting the...

Samah A. Mokbel, Ahmed K. El-Attar

...ment represented a short method for high level
management. To investigate the effect of gamma rays to produce healthy plantlets, the
hibiscus witches' broom phytoplasma was transmitted by grafting into healthy
hibiscus plants, the in vitro-infected hibiscus explants were exposed to gamma
radiation at different doses - 5 gray (Gy), 10Gy, 15Gy, 20Gy and 25 Gy- emitted
from cobalt 60 (60Co) for 30 minute...

Samah A. Mokbel1, Ashraf A. Abd El-Mohsen2

... respectively. DAS-ELISA method was applied to the tubers directly, in vitro cultures and young acclimatized plantlets to detect these viruses. After direct transplant of in vitro virus-free plantlets in greenhouse. The statistical analysis showed that cultivars and ratio of bed components and their interaction had insignificant (P>0.05) effects on minituber number and minituber weight. However, ratio of bed components and interaction with cultivars were si...

Rehab A. Dawood1; Entsar A. Nassar2; and K.A. El-Dougdoug 3

...ic of MS medium is a new method for Potato virus Y (PVY) elimination using tissue culture technique. Osmotherapy refers to culture meristem – tip and stem nodes at MS medium saturated with sucrose. Stem nodes of potato were successfully stored for up to 12 month at osmo-preservation medium supplemented with sucrose 50, 60 and 70 gl -1 at 210C. The survival percentage was recorded 85.1, 81.2 and 50.8 for 50, 60 and70 gl -1 respectively for 12 months at 21...

Elharony, S.B.1,3, SaharA.Youssef2 and Richard F. Lee3

...nd applicable extraction method for the isolation of
high quality nucleic acid (DNA and RNA) to detect all graft
transmissible pathogens of citrus including prokaryotic pathogens,
DNA and RNA viruses and viroid provides a new approach to virus
and virus like detection and identification. The method provides a
pure preparation of un-degraded RNA and DNA in high yield...

Samah A. Mokbel, Soad H. Taha, Mahmoud M. Abd-El Hamid, Ali H. Hamed

...roved to be an effective method for PVX-inhibition as compared with other concentrations. Also, results of antiviral activity of lactoferrin at concentration 1000 mg/L showed great potential as phytotherapeutic source to produce quality and health plantlets for rapid and large scale in vitro production.

...

Maryam Yousaf1, Salman Ahmad1*, Romana Anjum2 and Muhammad Zeeshan Majeed3

... conidia/mL by drenching method. Untreated plants served as control. First disease symptoms appeared after seven days of inoculation and seedlings became wilted within 30 days post inoculation. Stems from wilted as well as control seedlings were cross sectioned to observe the histopathological modifications. Wilted cross sections represented the cell wall disintegration and disruption of the tissues along with the blockage in xylem and phloem vessels due to th...
Eman A. Ahmed1, Osama Y. shalaby2, Emad F. Dwidar2, Samah A. Mokbel1 and Ahmed K. El-Attar1
...e secondary walls. Three methods were done towards the production of phytoplasma-free tomato plantlets through tissue culture using antibiotic compound (tetracycline hydrochloride in three concentrations 25mgL-1, 50mgL-1 and 75mgL-1), irradiation by three doses (3, 5 and 10 Gy) of gamma ray and natural compound (1ml of garlic juice). All treatments proven to be a very useful effect against phytoplasma, except concentration 25 mgL-1 of tetracycline hydrochlorid...

Radwa M. Shafie 1 and Soha S. M. Mostafa2

...e pattern using SDS-PAGE method. The contents of alkaloids, phenols and terpenoids in both algal filtrates and biomass were determined.

...

Asfand Raheel1, Syed Zulfiqar Ali2, Muhammad Waris2, Muhammad Basharat2, Basheer Ahmed2, Muhammad Arshad Ullah3, Syed Ishtiaq Hyder4 and Taqi Raza4*

Lamya A. F. Ateya1, Said A. Ahmed 2, Mansour H. Ayman3, Khamees K. Ashraf 4, Heba A. Abdel-Hady 5

Ola Youssef 1; EL-Deeb A.H.2 Nassif S.A.1 and Ahmed A. El-Sanousi 2.

Ehab M. El-Nahas1, Hemat S. El-Sayed2, Sawsan S. El-Basuni3, Gabr F. El-Bagoury1

Ayman S. El-Habbaa 1, Gabr F. El-Bagoury1, Samar F. El-Adaway2, and Susan S. El-Mahdy2

Manar F. Seioudy1, Magda M. Sayed1, Ahmed A. El-Sanousi2 and Mohammed A. Shalaby2

Muhammad G. Khodary1, Ayman H. El-Deeb2, Mohamed M. Emara2, Othman E. Othman1, Hussein A. Hussein2

Lamiaa M. Omar, Mohamed A. Abdrabo, Dali M. Omar, Nermeen A. Marden

...ation (VI) and titration methods
in (ECE) or by molecular assay for detection AI virus specific nucleic acids such as (rRT-PCR).
Methods: Titration of the HPAI virus for infection of SPF chicken. The tracheal swabs were collected
from diseased chicken for virus isolation and quantification by ECE inoculation and rRT-PCR.
Results: the titer of original HPAI virus was 1010.3 EID5...
Rasha M. Mahrous1,4, Ahmed K. El-Attar2, Ahlam A. AlWatban1, Sohair I. EL-Afifi3,
Nagwa M. Aref1,3

Ahmed A. Azab, Wesam H. Mady, Ali Zanaty, Mahmoud Samir

...n of different isolation methods was done
by generating a standard curve by real time PCR.
Results: The results revealed that the propagated virus in CEK showed the highest virus titer (5.952 x
106 and 3.245 x 106) than Vero cell line (7.184 x 101 and 2.14 x 103) and ECE (7.536 x 103 and 5.444 x
105) after both 48 and 72 hours respectively.
Conclusion: The chicken embryo kidney cell (CEK) is the most ...
Ashraf S. Khameis, lamya F. Atteya, Ayman H. Mansour, Heba A. Abdelhady, Ashraf A.
Saad

Dina A. Abdulrahman1, Ayman H. EL-Deeb2, Momtaz A. Shaheen1 & Hussein A. Hussein2

Dalia M. Omar1, Nermeen A. Marden1, Lamiaa M. Gaafar1, Elham A. El-ebiary1, Khalid El-Dougdoug2, Badawi Othman2, Abd Elsattar Arafa3, Hussein A. Hussein4

Nermeen A. Marden1, Lamiaa M. Gaafar1, Hussein A. Hussein2

...ironment. The evaluation methods of the AI vaccines are very important
for controlling the license of the bad vaccines. Comparative studies between the invitro and invivo
methods for 8 types of AI vaccines (5 batches for each) to evaluate their efficacies were done.
Methods: The invivo method, was done through the vacc...
Ruqayya Bint Khalid1, Asif Nadeem1,2*, Maryam Javed1, Muhammad Zubair Shabbir3 and Masroor Ellahi Babar4
...m whole blood by organic method. PCR primers were designed and optimized according to respective melting temperatures. PRALINE tool, MEGA 6.0 and POPGENE tool were utilized for phylogenetic tree construction and statistical analysis, respectively. Characterization of physical and chemical properties of β-casein protein was performed by ProtParam and SWISS MODEL was utilized for protein model prediction. Sequencing results of amplified PCR products reveale...
Sahar Mubashar*, Tariq Mukhtar and Nasir Ahmad Khan
...ngi and viruses. Various methods are used for the detection of the virus such as nucleic acid and immunological methods but RT-PCR is considered as the most reliable. Some antiviral drugs have shown to be effective against the virus like Favilavir, Remdesivir, Chloroquine, hydroxy-chloroquine, Tocilizumab etc. but further clinical studies are required to confirm their efficacy. In Pakistan, blood plasma therapy is in high de...

Akram I. Aboelkhair1, Ayman H. El-Deeb2, Momtaz A. Shahin1 and Hussein A. Hussein2

Nagwa K. Meselhy1, Mohamed A. Abo El-khair1, Basem M. Ahmed2, Sherif A. El-Soally3,
Abdelhamid M. Fares1, Mohamed A. Nayel1 and Hussein A. Hussein2

Hanaa A. Elsamadony1, Laila A. Tantawy1, Sabry E. Omar2 and Heba A. Abd Alah1

...>
Egypt to improve methods of virus control and to usually determine the relationship of circulating
CAV with vaccine strains and other CAV strains. The most obvious thing in this study, that’s we need
to make our vaccine from our strain which circulating in the field with periodic updating.
...
Othman N. O. Mansour 1, Naglaa Hagag 2, Momtaz A. Shahein 1, Sayed S. Hassan 1 Ahmed
A. EL-Sanousi 3 and Mohamed A. Shalaby3

Rana A. Rabiea1, Mohamed Fawzy2, Mohamed H. Khodeir3 and MokhtarM. EL-Tarabili2

Mohsen M. Elsharkawy1, Salem H. Homayed1, Mohamed M. Elsawy2 and Amr A. Khedr1

Allam A. Megahed, Badawi A. Othman, Samar S. El Masry, Abeer A. Faiesal and Mohamed A. Nasr Eldin
Samira M. Bolis1, Magda Mahmoud2, Salah E. Gumma3, Naser E. Bilal1 and
Isam ElKhidir4
Riham H. Bassam, Hussein A. Hussein, Mohamed M. Amer, Basem M. Ahmed and Ahmed A. El-Sanousi

Riham H. Bassam, Hussein A. Hussein, Mohamed M. Amer and Ahmed A. El-Sanousi

Aml S. El-Saadany1, Ayman H. El-Deeb2 and Hussien A. Hussien2

Mohamed F. ElKersh1, Fatma M. Abdalla2, Gamilat K. Farg2, Soad A. Nasef3, Ahmed A. Ali2

Mohsen M. Elsharkawy1, Sara E. Hanbal2, Samir A. Sidaros1, Mohamed M. Elsawy2, Ali H. Hamed2

...um SKT1 as a sustainable method to control the invasion of BYMV in faba bean plants.
Methods: Faba bean plants were treated with PGPF isolates at 2 days prior to BYMV inoculation. Disease severity and virus titer were estimated at 1, 2 and 3 weeks after virus inoculation. The transcription profile related to defense genes was evaluated at 1, 2 and 3 weeks after BYMV inoculation by quantitative real-time PCR analy...

Shimaa A. Khalaf1 , Mohsen M. Elsharkawy2, Samir A. Sidaros2, Shawky A. El Kewey2, Ayman F. Omar2, Ali Hamed1

Huda S. Darwish 1, Om-hashem M. El-Banna2, Mohamed S. Abbas3, Hoda M. Waziri 1, Maisa A. Awad1
...tagens by ISSR molecular method.
Methods: ToRSV was isolated from infected tomato, grapevine and pelargonium plants and detected
using double antibody sandwich-enzyme linked immunosorbent assay (DAS-ELISA). Host range study
was carried out using mechanical inoculation into fourteen different diagnostic host plant species and
cultivars belonging to 6 families. Tomato seeds were ...

Wesley D. Nafarnda, Japhet H. Baraya, Olatunde H. Olabode, Malang K. Simon

...umented as a
method for control of the disease with varied effects in scavenging rural poultry especially in Nigeria.
Aim: This cross sectional study was conducted to establish the impact of V4 thermostable ND
vaccination on rural poultry productivity using corn bran (dusa) as alternative vaccine carrier in five
Local Government Areas (LGA) in Adamawa state, Nigeria.
Method
Hadeer M. Mossa, Ausama A. Yousif, Emad A. Aboelsoud, Mahmoud El Gamal, Ahmed El-
Sanousi
...faster and more accurate methods to
analyze, characterize and qualify the virus as a seed for future vaccine production. Analysis workflow
starts on a high performance laptop device (GENUS), sequence data is generated for bacteria, viruses,
fungi and archaea that present in the sample it was then classified to subspecies and strain level in a
quantitative manner in approximately 48min and 23 second.
C...
Ahmed A. ElWakil1, Ausama A. Yousif2 , Adel A. Fayed3, Ibrahim M. elsabagh2, Mahmoud
El Gamal2, Omnia H. Refaei3

Mohamed M. Mashaly1, Ayman H. El-Deeb2, Momtaz A. Shahein1, Hussein A. Hussein2

Soad, E. Elsayed 1, Mohammed A. Shalaby2, Ausama A. Yousef2, Abu bakr A. Agor1,
Sherouk E. Aly1
...conomic impact. The main method of control is through vaccination of the
livestock with a potent vaccine.
Objective: Process development of the current formulations and generating new ones is crucial.
Currently, Montanide ISA 206 is the adjuvant used in FMDV vaccine produced in Egypt which is
w/o/w microemulsion.
Methods: We aimed to increase the efficacy of the con...
Shourok E. Aly 1, Karim Zaki1, Soad E. Elsayed2, M. Elgamal2, Ausama A. Yousef1, Mohamed A. Shalaby1
Karim M. Selim1, Abdullah A. Selim1, abdelsattar Arafa1, Hussein A. Hussein2, Ahmed A. El-Sanousi2

Ljupco Angelovski*, Zagorka Popova, Katerina Blagoevska, Sandra Mojsova, Marija Ratkova Manovska, Mirko Prodanov, Dean Jankuloski, Pavle Sekulovski

...entional microbiological method and with the use of multiplex PCR method. Both methods confirmed the overall prevalence of C. jejuni of 39.2%. In the second part of the study 108 confirmed isolates of C. jejuni were analyzed for the presence of resistance genes (CmeB, Blaoxa-61, tet(O), aph-3-1 and aadE). The analysis in the third part of the study was concentrated on the antimicr...

Ayesha Manzoor1, Muhammad Saqib Naveed1, Sayed Rashad Ali1, Danish Ibrar2, Sairah Syed1, Sharmin Ashraf1 and Rafiq Ahmed1*

...op species. Root to seed method is widely used in Pakistan for radish seed production but still low and inferior quality seed is being produced that cause a significant loss in yield due to lack of standardization of seed production technology.Therefore, to improve seed yield and quality, present research was conducted toevaluate the effect of steckling size on plant growth and seed yield of radish.Steckling of different lengths (5cm, 10cm, 15cm) and width (4....

Mian Ibad Ali Shah*, Yasir Saleem Afridi and Laiq Hasan

...is comprising of various methods and factors have been used to infer conclusions from data. Some important factors like, stringency index, number of tests, etc. have been utilized in this paper to achieve the aforementioned goals. 
...

Iftikhar ud Din* and Yousaf Hayat

... widely used statistical methods for assessing the differences among the means of more than two treatments by considering single trait independently. In reality these traits are not independent and appropriate techniques for analyzing multiple traits simultaneously, is the multivariate analysis of variance (MANOVA). This study deals with the illustration of MANOVA using simulated data, related to agricultural trials. The appropriate design,

Ghani Akbar*, Muhammad Asif and Zafar Islam

...itional open-air farming method in Potohar, a rainfed area in Pakistan. The results indicated increased production potential per unit of land (46%) and per unit of water (74%) and larger farm income (3.47 BCR) than the traditional open-air farming method. These production benefits can be instrumental in improving livelihood and nutrition of remotely located rain-fed farmers in the country in general and Potohar region in par...

Nusrat Habib

...hange with a qualitative methodology. Focus group discussions, participatory observations, and key informant interviews were used in collecting women’s perspectives. Flash floods, temperature rise, erratic rainfall, and land sliding were the main climatic hazards perceived by women. During the risks, the most vulnerable people were women and children. Women’s household activities burden doubled during the disaster days comparing with their male mem...

 Yongmin Li1,2, Huabin Zhang1, Xiaoyou Wu1, Dongwei Li 2, Peng Yan1 and Xiaobing Wu1*

... Bayesian inference (BI) methods supported the sister-group relationship between ((Rhacophoridae + Mantellidae) + Ranidae) and Dicroglossidae. Within Rhacophoridae, two species of the genus Rhacophorus (R. schlegelii and R. dennysi) were clustered together with the representative of the genus Polypedates (P. megacephalus), meanwhile, the representative of the genus Buergeria (B. buergeri) occupied the basal posi...

Syed Ismat Hussain1, Khalid Mehmood2, Mudassar Khaliq3, Habib Anwar1, Syed Muhammad Zaka4, Ateeq ur Rehman5*, Muhammad Shahid6, Syed Atif Hasan Naqvi5*, Ummad ud Din Umar5 and Muhammad Asif Zulfiqar7

...ays model, Huber’s method was adopted and data for calculation of generations by accumulating progressive degree days was observed highly significant i.e., P>0.0001. In the present study, the accumulation of degree days for its forecasting was calculated from 1st January by using the metrological data and base line temperature and found 7 generations of pink boll worm to be found in all districts. Data for moths trapped in sex pheromone traps was note...
Muhammad Noman Koondhar1, Asghar Ali Kamboh1,*, Muhammad Ammar Khan2, Riaz Ahmed Leghari3 and Parkash Dewani4
...using the disk diffusion method. The results indicated that 21.34% beef samples exhibited the Salmonella contamination. The statistical analysis exhibited a significantly higher (p< 0.05) prevalence rate in minced beef (28%) and lymph nodes (24%) as compared to the muscles (12%). The highest antimicrobial resistance was recorded against the antibiotics of penicillin group (88.5 to 93.7%), fol...
Houqiang Luo1*, Yanfang Lan2, Ping Gan3, Wenjun Zhou4, Meng Wang1
Bing Hu5, Zhuning Zhang5, Yu Bai1* and Kun Li6*
... and molecular detection methods were used to identify the tick species and CME by amplification of the cytochrome oxidase subunit I and disulfide oxidoreductase gene, respectively. The results indicated that 1.29% of the serum samples were positive for E. canis, and 5.50% of dogs were infested with ticks. The Wenzhou samples of R. sanguineus exhibited a high homology (99.7%–99.8%) and these parasites showed a 99.1%–100% homology to p...

Muhammad Jan1*, Muhammad Ashraf Sumrah1, Shoaib Akhtar1, Inam Ul Haq2, Muhammad Ramzan Anser1 and Iqra Yasmin1 

..., right source and right method. In case of soil application, apply first dose of nitrogen, all phosphorus and all potassium fertilizers during the December. Apply second dose during May-June and third dose during September in Pothwar. Foliar application is suitable during end February and March. Drip irrigation is suitable during December and May.

...

Sana Javeid* and Khalid Nawab

...gents, extension contact methods, strategies and trainings showed significant relationship with poverty. Hence, it is recommended that efforts are needed to regularize all extension activities for poverty reduction in the study area.

...

Moheem Khan1, Arif Ali1*, Shafique Ahmed Memon1, Taimoor Khan Qambrani2, Ghulam Khaliq3, Jahanzaib Khan1, Saghir Ahmed1, Azmat Hussain Abro4 and Sohail Azeem Baloch1

...iation is an established method for controlling insect pests in store-grain and a significant stress factor affecting stored grain pests. The Present research work was designed to control T. castaneum through ultraviolet radiation (UV-B 313 nm) light is applied at various timings such as 0, 5, 10, 20, 40 and 60 minutes UV-B light on all developmental stages of T. castaneum. The results revealed that significant mortality was observed in eggs, larvae and pupal ...

Gulzar Ullah* and Gohar Ayub

...pre-transplant hardening method. Both groups HP (heat pretreatment) and NHP (Non-heat pretreatment or Control) were then transplanted to the field. Results revealed that different heat pretreatment durations and varieties significantly affected the growth and yield of tomato. Regarding heat pretreatment durations, number of flower clusters plant-1 (16.06), fruit set percentage (68.33%), number of flowers cluster-1 (8.34), chlorophyll content (50.31 SPAD), leaf...
Zunaira Noreen* and Khawar Sultan
...18 using the point count method for bird census determined the baseline for 56 species (birds/10 min) indicating a significant change in native avian composition. PCA analysis showed four clusters based on the nature of habitat and species behavior. Agriculture land was found to be the highest (H’= 3.394) and landfill the lowest (H’= 1.414) in biodiversity. However, landfill site registered the most number of birds (>9000). Among twenty species ...
HuaBin Zhang1,2, YaSen Li1, Tao Pan1, Peng Yan1, En Li1, Hui Xue1 and XiaoBing Wu1,*
...ator by radioimmunoassay method. Our results demonstrated circulating leptin levels varied significantly over the season. Therefore, we hypothesize that leptin is involved in nutrient stores, absorption and utilization, and reproductive behavior of the Chinese alligator.
...

Kawa A. Ali1*, Sazar S. Noraldeen2 and Arshad A. Yaseen2, 3

...se) to indicate the best method of chlorophyll estimation. After 80 days from sowing chlorophyll estimation was implemented first by nondestructive methods using SPAD (SPAD-502 Plus Konica Minolta Tokyo, Japan) and atLEAF chlorophyll meter (at-LEAF FT-GREEN-LLC Willington, DE, USA) instruments, then the same leaves were cut and chlorophyll estimation were done by destructive methods using ...
Chaochao Hu1,2, Xue Xu1, Wenjia Yao1, Wei Liu3, Deyun Tai1, Wan Chen4 and Qing Chang1*
...s by at least one of the methods. This study provides a valuable resource facilitating further study of phylogenetic and evolutionary analysis for wader, and improves our understanding of the evolutionary and taxonomic research within Charadriiformes.
...
Zhongyong Wu1, Wenzhu Li2, Chunxia Ni1, Zhuolin He1, Xiao Hou1 and Wanxia Pu1*
...d polymorphic DNA (RAPD) method with three oligonucleotide primers. In total, 177 Staphylococcus aureus isolates were obtained from dairy farms in 5 different provinces in China, including Guizhou, Inner Mongolia, Shanghai, Sichuan, and Gansu. The results showed that the amplified product bands with three primers per isolate ranged from 1~9 and the product sizes from 240 to 4,500 bp. Primer OLP13 had the highest discriminant coefficient. The Staphylo...
Zhi-Ming Luo*, Xiao-Yan Wang, Jiong Yin, Ying-Kun Huang, Wen-Feng Li, Rong-Yue Zhang, Hong-Li Shan, Xiao-Yan Cang and Jie Li
...This study established a method to assess sugarcane seedling resistance to borer species that could be used in future resistance evaluations of novel sugarcane varieties during the crop breeding process.
...
Aqsa Shahid1, Saima Muzammil1, Farhan Rasheed2,Bilal Aslam1, Muhammad Akhtar Ali3, Syed Zohaib Haider4, Masroor Ellahi Babar4, Muhammad Saeed1, Umair Waqas1 and Mohsin Khurshid1,*
...mined by the Kirby-Bauer method and minimum inhibitory concentration (MIC) to aminoglycosides was evaluated by the broth microdilution method. Moreover, the armA genes were studied by PCR followed by Sanger sequencing. The isolates showed a high rate of resistance to cephalosporins, carbapenems, aminoglycosides, and fluoroquinolones whereas colistin and tigecycline were the most active antimicrobial agents against ...

Fahima Khatun1, Abdullah-Al-Maruf2, Md. Mizanur Rahman2, Afroja Yasmin1, Mohammad Ali Zinnah3, Md. Aminul Islam4 and Mohammad Shah Alam5*

...examined by direct smear method and helminths identified by the presence of characteristic eggs in the feces. This study was carried out with three age groups: calves (<1 year), young (1-3 years), and adult (>3 years) and three different consecutive seasons (winter, summer, and rainy) during the periods of January 2018 to December 2018. The highest incidence was found in infestation with Fasciola spp. (43.63%) followed by Toxocara spp. (35.75%) and Haemo...
Muhammad Bilal Tayyab1, Muhammad Zeeshan Majeed1*, Muhammad Asam Riaz1, Muhammad Anjum Aqueel2, Sylvain Nafiba Ouedraogo3, Muhammad Luqman4, Kanwer Shahzad Ahmed1 and Mujahid Tanvir1
...ip and leaf-dip bioassay methods, respectively. Results of initial screening bioassay showed the highest mortality of D. citri by 10% extracts of Mentha longifolia (L.) Huds. (93%), Melilotus officinalis (L.) Pall. (91%), Nerium indicum Mill. (89%), Datura alba L. (88%) and Salvia officinalis L. (81%). Second bioassay conducted against A. gossypii using different concentrations (5, 10, 20 and 40%) of the most effective botanical extracts revealed that the extr...
Rosidah Rosidah1*, Angga Nugraha1, Yuli Andriani1, Kiki Haetami1, Olga Anne2 and Agus Heri Purnomo3
.../i> (Chester, 1901). The method used was the experimental method using a completely randomized design with five treatments and three replications was applied. The treatment used was immersion with A. vera extract with a concentration of treatment A 
(0 mg ...
Naveed Farah1*, Izhar Ahmad Khan1, Asif Ali Abro2, Jehanzeb Masood Cheema3 and Muhammad Luqman4*
...nce of Pakistan. A mixed method approach was employed for data collection and at first, a longitudinal analysis of Landsat imagery of land use was done through GIS and Remote Sensing for a time period of 2001-2016. The results of GIS analysis revealed that during the period 2001 to 2016, Faisalabad showed 24% increase in urban area and 23 % of this expansion consumed agricultural land. In Sahiwal, urban land has increased by 21 %, which encroached 13 % of agri...

Najam-un-Nisa1, Ruqia Bibi1, Balqees Riaz1, Bushra Khalil1, Iqra Maheen1, Saima1, Uzma Islam Khan1 and Inam Ullah1,2*

...017-1019. Point transect method was used to explore the avifauna diversity at study site. In current study, total of 13,933 birds belonging to 39 species, 21 families were observed. Among these 39 species, 33 bird’s species were terrestrial and the rest of 21 bird’s species were aquatic. In water birds species, Great Egret is the most abundant specie of 2017-18 year, while in 2018-19 year, most abundant specie is Little Cormorant. In forest birds s...

Hafiz Muhammad Tahir*, Iram Liaqat*, Junaid Nadeem, Hammad Aamir and Shaukat Ali

...ation and disk diffusion method to investigate the antibacterial potential of Neoscona mukherji and N. theisi egg and web silk. For disk diffusion method, silk was first degummed to form degummed silk solution’ (DgS), then the degummed silk was dissolved to form the dissolved silk solution (DSS). The DgS and DSS solutions were tested against selected common pathogenic bacteria (Esherichia coli, Salmonella typhimurium S...
Jun-Dong Tian1,2, Wei-Jie Guo1,2, Han Yan1, Jie Zhang1, Shu-Liao Tian3 and Ji-Qi Lu1,2,*
...ed non-invasive sampling method to collect the feces defecated in the morning (AM group) and afternoon (PM group) from a zoo rhesus macaque population. Then 16S rRNA sequencing technology was adopted to assess the microbial communities. The results showed i) the dominant phyla were Firmicutes, Bacteroidetes, Proteobacteria and Spirochaetace accounting for over 86 % of the richness of whole microbiota, ii) Ruminococcaceae, Prevotellaceae, Lactobacillaceae and S...
Liyun Chang1, Yingbin Chen1, Qian Kang1, JianhuaQin1,* and Zhiyong Liu2,*
...sing Ct value comparison method (RT-QPCR2-ΔΔCt). The primers were designed according to the sequence of mouse cytokines in GenBank. The expression levels of the cytokines were normalized to GAPDH gene expression. Fluorescence threshold (Ct value) obtained from RT-QPCR2-ΔΔCt method with GAPDH standard curve (linearity R2=1), followed by sequencing analysis showed that a...
Muhammad Arslan Akbar1, Khalid Javed2, Asim Faraz3* and Abdul Waheed3
...at of simple correlation method. PCA was used to ascertain the important morphometric traits having greater variation and these traits can be helpful for improvement and in formulation of breeding plans and selection criteria for elite animals.
...

Usman Asghar1*, Muhammad Faheem Malik1, Umer Rashid2, Naeem Mahmood Ashraf2, Sumera Afsheen1 and Muhammad Hashim1

...DNA-based identification methods are ideal and reliable. DNA was extracted from muscle tissues of cow, buffalo, goat, donkey and a dog. A set of degenerative primers was designed for Polymerase Chain Reaction (PCR) of the cytochrome b gene fragment. All PCR was accomplished at the same reaction conditions. Amplified products had a length of 400 bp, which was our estimated length. After the sequencing of these amplicons, In silico restriction analysis was perfo...

Tajnees Pirzada1*, Weenghar Ali Chandio1, Mansoor Ali Kalhoro2, Mir Munsif Ali Talpur1, Waheed Ali Mirbahar1, Abdul Ghafar Solangi1, Zulfiquar Ali Jumani1 and Rehana Kerio1

...nation. A simple one-pot method was used to synthesize HA/ZnO NPs involving zinc oxide nanoparticles (ZnO) core (20-35 nm in diameter) and humic acid shell. HA/ZnO NPs were used to investigate the effect on the germination profile of Brassica campestris. Germination profile parameters were measured as root-shoot length, germination index, fresh and dry weight for 15 days. HA/ZnO NPs were confirmed with various physicochemical techniques such as X-ray diffracti...

Muhammad Yaseen1*, Muhammad Sallam Shahzad2, Farhat Ullah Khan2, Muhammad Luqman1, Usman Saleem3 and Shoaib Nasir4

...training as an extension method for promoting rice production technology was rated first. Weeds problems were ranked first among the problems faced by farmers for rice production. The data also showed that insect pests and disease problems were ranked the second problem. The study suggested that the public sector advisory services providers must address different hurdles hampering the successful development and execution of the latest rice production strategie...
Farhat Iqbal1, Abdul Waheed2*, Zil-e-Huma3 and Asim Faraz2
...radient boosting machine methods have been used to predict the live body weight (target variable) of the animal. The predictors (independent variables) included in the current study were sex of the animal, neck length, diagonal body length, head girth above eyes,shank circumference, belly sprung, and rump height measurements of beetal goats (1 – 7 years in age) of Pakistan. In order to test the performance of candidate method
Madiha Nawaz and Shoaib Freed


... conditions by immersion method. Three entomopathogenic fungi; Beauveria bassiana (isolates Bb-01, Bb-08), Metarhizium anisopliae (isolates Ma-11.1, Ma-2.1) and Isaria fumosorosea (isolates If-2.3, If-02) showed percent mortalities of (61.0%, 85.0%), (78.0%, 56.0%) and (52.0%, 54.0%) with LC50 values of (4.25×108, 2.54×108 spores/ml), (3.26×108, 5.08×108 spores/...
Ishtiaq Ahmad1*, Abid Usman1, Khursheed Iqbal1 and Shams Ur Rehman2
...ultiple regression based methodology was developed to use fixed-effect model for both dependent variables Return on Assets and Tobin-Q variables to discover the association between Corporate Governance and Firm Performance. It is concluded that Board Size, Board Gender, Board Experience and firm size have significant the ROA and Chief Executive Duality, Board Size, Board Gender, Board Experience, and Firm size have significant with Tobin-Q.
...

Mohammed Abdel-Mageed Abdel-Aziz Abdel-Mageed1, Eman Alsayed Hammad2*, Nashaat Abdel-Aziz Mahmoud1 and Anas Farag El-Mesalamy1

... varied according to the method of applications and the type of materials. Adding the extract with the chelating nutrients improved the properties of the plant, but adding the extract as doses to the soil reduced the numbers of nematodes better than other methods of application.

...

Mst. Deloara Begum1, Md. Muniruzzaman1, Md. Salauddin2* and Md. Mostafizer Rahman1

...cteriological laboratory methods and purified isolates were identified according to gram’s staining reaction, colony morphology, cultural characteristics, biochemical and antibiotic susceptibility test. All most 100% dried and 20% cooked fish sample were contaminated. In this study seven different species and 168 isolates were identified from dried fish and these were Escherichia coli 21.43% (36), Vibrio spp. 18.45% (31), Staphylococcus spp.17.86% (30), ...
Yu Yang1,2, Shengqiang Ye1, Lixia Wang1, Xing Chen1, Yunguo Qian1 and Ping Gong1,*
...nohistochemical staining method. The result showed that DMRT1 was mainly expressed in spermatogonia nucleus of seminiferous tubules and had no expression in Leydig cells. It was indicated that the DMRT1 expression was positively correlated with the testes development of adult mule duck.
...

Ruqia Bibi1, Najam-un-Nisa1, Sania Komal1, Hafiza Nimra Ghani Qureshi1, Maryam Safdar1, Hafiza Hina Jameel1, Saman Gul1, Sania Saeed1 and Inam Ullah1,2*

...-2019. The line-transect method was used to explore the avifauna diversity at the study site. 16371 individuals were observed belonging to 11 families belonging to approximately 38 species during the present study. The relative abundance was found to be 1, the Shannon-wiener diversity index (H´) was calculated as 3.43, the Simpson index (D) was 0.96, and the Evenness was 0.35. Little Cormorant (Microcarbo niger), Gadwall (Mareca streper), Great Cormorant...

Muhammad Usman Hanif, Abu Bakar Muhammad Raza, Muhammad Zeeshan Majeed*, Muhammad Arshad, Muhammad Irfan Ullah

... using standard leaf-dip method. Results revealed that spinosad and 10% A. indica aqueous extract exhibited maximum mean cumulative mortality of hadda beetle grubs (i.e. 84.1 and 73.4%, respectively) and adults (i.e. 67.4 and 58.8%, respectively) recorded at 5th day of exposure. Combined application of both these effective treatments enhanced their toxicity to E. vigintioctopunctata and caused 88.9 and 77.6% cumulative mortality of grubs and adults, respective...

Ayesha Khan1*, Asmatullah2, Urooba Perviaz1, Khalid Nawab1 and Mahmood Iqbal1

... fertilizers alternative methods are adopted to decrease use of fertilizers. One of the techniques for organic farming is EM-Technology. EM-Technology is used in addition with animal manure and other organic wastes effectively in agriculture. The universe of the study was Malakand division of Khyber Pakhtunkhwa. The EM-Technology using 128 farmers’ data were gathered from the District Director Agriculture of six districts. Each farmer was interviewed fac...
Misbah Sarwar1*, Abdul Hamid2 and Iftikhar Hussain2
...s employed both of these methods to capture cranes. In total, 7947 cranes were captured during the study period. Age wise segregation showed that 6624 (83.4%) of captured cranes were adults and 1323 (16.6%) sub-adults. Among the adult cranes, 3279 (49.5%) were males and 3345 (50.5%) females. Cranes captured during spring and fall from 2012 to 2015 included 1021 and 502; 981 and 527; 787 and 311 and 843 and 462 respectively. The difference between the spring an...

Samreen Khan*, Salma Javed, Tabassum Ara Khanum, Nasira Kazi

.... One of the alternative method against insecticides for better management of plant health under greenhouse and field circumstances in integrated pest management program. Being potential of these nematodes as biopesticides may be distributed to farmers to reduce use of insecticides already in practice and to control hazardous effect of chemical pesticides on both crops and human beings.
 
Novelty Statem...
Funda Turan1*, Ihsan Akyurt2 and Sehriban Cek-Yalniz1
...ere applied by immersion method on C. gariepinus larvae for 30 days, and the effects of red clover extract on sex reversal and gonadal development were also examined at the end of 120 days.At the end of experiment, highest feminization (89%) was observed at 50 mg L-1 red clover group. Morphological and histological examinations of the gonads in all groups revealed no intersex fish. Histological examination of fish treated with red clover extr...
Farah Deeba1, Hafiz Abdullah Shakir1, Muhammad Irfan2, Muhammad Khan1 and Javed Iqbal Qazi1*
...sign of response surface methodology. The maximum chitinase production was achieved employing 5% chitin, 0.5% rice straw, 0.05% peptone, 0.02% CaCl2, and 0.05% yeast extract. The utilization of agro-industrial waste (rice straw) not only decrease the production costs of microbial chitinase but can also providing positive way out for solving environmental pollution problem related to the waste.
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Najeeb Ullah1*, Muhammad Nadeem2, Malik Mujaddad-ur-Rehman1, Rubina Nelofer2 and Yasir Arfat3

...results Response surface methodology (RSM) was employed to optimize five different independent variables including substrate, Molasses, temperature, pH and incubation period. Maximum protease activity was observed by using molasses (540.62 ± 26.77 IU/ml) as carbon source and NaNO3 (606.95 ± 7.61 IU/ml) as nitrogen source. The optimum conditions predicted by RSM were substrate 2%, molasses 1.5%, temperature 35 °C, pH 9.0 and induction period o...

Eman H. Mahrous1, Mohamed. W. Abd Al -Azeem2, Faisal A. Wasel1, Waleed Younis2* 

...t and other conventional methods of identification. Recovery of P. multocida isolates of diseased rabbits from lung, liver, and heart were 23 (46%), 11 (22%), 13 (26%), respectively, with a total incidence of 47 (31.3%). Biochemical reactions proved that 20 (42.5%) out of 47 isolates had the typical biochemical properties of P. multocida. However, only 10 isolates were identified as P. multocida using species-specific primers by conventional PCR with an incide...

Hailemariam Assefa1*, Tesfahun Dagnaw2 

... oil by r disk diffusion method. Escherichia coli, Klebsiella pneumoniae, Staphylococcus aureus and Streptococcus pyogenes were used as tested bacterial species whereas Yeast, Mold and Dandruff were selected as tested fungi. The effect was seen high on Staphylococcus aureus, while the Klebsiella pneumoniae was less affected by any of the used extracts. Methanolic extract was more active than petroleum ether and ethyl acetate extract on all three tested fungal ...
Nabila Rasheed1*, Bushra Waseem1, Kanwal Haneef2, Shumaila Usman3, Madeeha Sadiq1, Tabinda Urooj1, Sarah Tarique2 and Dabeeran Zeha4
...by trypan blue exclusion method and wound healing potential by scratch assay method. Healing of the scratch was observed at different time intervals (0h, 6h, 24h, 48h). The images were analyzed by Image J software. Morphological analysis was done under inverted microscope. Results showed that with each passaging stage the viability of GDM cells remained normal whereas Wound healing potential of GDM-WJMSCs was decreased as co...

Muhammad Zeeshan Majeed1*, Muhammad Irfan Ullah1, Dilbar Hussain2, Muhammad Luqman3, Muhammad Qasim1, Gulfam Yousaf1, Hamza Latif1 and Muhammad Zeeshan1

...ilter paper-dip bioassay methods were used against sap-feeding pests and termites, respectively. Factorial analyses of variance revealed a significant impact of tested insecticidal formulations and exposure time on the mortality all insect pest individuals. The most effective differential chemistry insecticides against mango leafhopper, citrus psyllids, mango mealybugs and subterranean termites were nitenpyram, clothianidin, thiamethoxam and imidacloprid (caus...

M. Hanafy1, Rehab Elhelw2, Soliman M. Soliman3, Sherif Marouf2* 

... conventional phenotypic method and genotypically by polymerase chain reaction (PCR). At the level of biochemical characterization, the incidence of Pasteurella multocida was 40.4%, and Mannheimia haemolytica was 7.1%. Using KMT1 gene for identification of the isolates for P. multocida and SSE gene for identification of the isolates for M. haemolytica, the results revealed that six isolates showing positive PCR for Pasteurella multocida and were subject to fur...

Prima Mei Widiyanti1*, Mirnawati B Sudarwanto2, Etih Sudarnika2, Raphaella Widiastuti1 

...id chromatography (HPLC) method. This study showed that no heating (control), heating at 72 oC for 15 seconds (high temperature short time pasteurization), and 89 oC for 1 second (higher heat shorter time pasteurization) did not affect on enrofloxacin antibiotic residue concentration, however heating at 121 oC for 15 minutes (sterilization) affected the antibiotic enrofloxacin. The stability of enrofloxacin antibiotic could result problem in public health. Ant...

Abbas M. Al-Azab1*, Ahmed A. Zaituon2, Khalid M. Al-Ghamdi3, Fahd M. Abd Al Galil4,5 

...(black holes) and manual methods were used for collecting adults and immature stages in five residential areas in Jeddah. A total of 796 adults of Aedes aegypti mosquitoes were collected. Out of these, 373 males (46.8 %) and, 423 females (53.2%). Out of 950 collected immature stages, 83.41% larvae and 16.6% were pupae. The obtained statistical analysis showed that the availability of the adult and juvenile stages of A. aegypti occurred throughout the year in d...

Ijaz Rasool Noorka1,2*, Bilal Ahmad Khan3*, Kafeel Ahmad4, Zafar Iqbal Khan4, Muhammad Ather Nadeem3, Amer Nawaz2, Tasneem Ahmad5 and Humayun Bashir4

...dard samples preparation methods were worker to check occurrence of the heavy rock’s metals in the forage and soil samples. The Atomic Absorption Spectrophotometer was used to check Iron (Fe). The results revealed that Fe were found in safe limit, as per WHO. 

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Asif Sadam1*, Rahmat Ullah Khan1, Sajid Mahmood1 and Juma Gul2

...d employing point counts method in urban area; agricultural area with small forest chunk and water body. Twenty four point counts were plotted in three well-defined locations such that each location was divided into eight concentric rings of 10 m radius from 0-50 m with an onward interval of 50 m. Thirty five avian species belonging to 23 families and 10 orders were recorded. However, One Way Analyses of Variance (ANOVA) indicated significant (P < 0.05) dif...

Rehman Shahzad1, Saba Irshad1*, Malik Saddique Mehmood1 and Faisal Amin2

...using Kyte and Doolittle method. High hydrophobicity of NS1 protein is also posing a potential for H9N2 virus to adapt in host, which might be contributing to the increase in pathogenicity of the circulating H9N2 virus.

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Yan Jiang1,2, Xia Tao3,4 and Hongxia Chen5,6*

...domized controlled study method was designed for this study. 92 patients with IMN in our hospital from December 2015 to December 2017 were divided into study group (n=46) and control group (n=46) based on a computer-generated random number table. On the basis of conventional treatment, the control group was given prednisone and cyclophosphamide treatment, and the study group was given rituximab-assisted prednisone and cyclophosphamide treatment, both of which ...
Amjad Ali1, Muhammad Amir2*, Bilal Ur Rehman2, Zafar Khan3 and Abid Munir1
 
...ally, appropriate proven methods are used for processing fingerprints in a sequential manner followed by the application of various encryption algorithms for ensuring protection of such biometric data. In fingerprinting, image processing techniques include: low-quality-fingerprint enhancement, normalization, binarization and thinning of fingerprint image followed by feature extraction. In this paper, a novel encryption method

Mustafa Ozan Atasoy

...een generated by various methods and some of these constructs have been made commercially available so far. The efficacy of HVT-vectored vaccines has been assessed either individually or in combination with other vaccine formulations; hence, the optimal competence of these vaccines against viral diseases has been reported. Based on the current knowledge, this article outlines the benefits and drawbacks of HVT-vectored vaccines in combating health-threatening p...

Kusuma Adhianto*, Satria Ibnu Lenanto, Akhmad Dakhlan, Muhamad Dima Iqbal Hamdani 

... Sumberejo District. The method used is a survey method, the research sample was determined by purposive sampling. The data were analyzed using the paternal half-sib correlation method. The observed variables included weaning weight and yearling weight. The results of this study showed that the average weaning weight of female Saburai goats in Sumberejo District was 16.4±2.0 kg, and...

Keqiang Wei1*, Yue Wei2 and Changxia Song1

...d an immunohistochemical method to investigate their cellular localization and expression in hepatopancreas of red swamp crayfish (Procambarus clarkii), a commercially important aquaculture species in China. Using polyclonal antibodies, the strong staining of these proteins in hepatopancreatic cells was observed. The synchronous expression of p38 and its phosphorylated form (p-p38) was mainly present in B- and R- cells, and their MOD values were 0.0055±...

Mirza Gul1*, Muhammad Zahid1, Ikram Ilahi2, Hazrat Ali2*, Fida Hussain3 and Muhammad Anwar Sajad3

... performed following WHO methods, with slight modifications. LC50 values for A. scoparia and A. indica against early fourth instar larvae were 360.4 and 971.1 ppm, respectively. LC50 values for pupae were 1665 and 2838 ppm for A. scoparia and A. indica extracts, respectively. Percent knockdown after 1 h exposure was 49.0 for A. scoparia. KDT50 and KDT90 values for A. scoparia were 69.7 and 763.5 min, respectively. LC50 values for A. scoparia and A. indica agai...

Khadim Hussain Memon1,*, Amir Mahmood Memon2, Tahira Jabeen Ursani3, Abdul Manan Shaikh1 and Sapna Waheed Memon1

...th function and ELEFAN-I methods to estimate the growth parameters. The asymptotic length (L∞) and the growth rate (K) were 82.95 cm and 0.390/year, respectively, wherein the hypothetical age at zero length (t0) and goodness of fit or score (Rn) -0.495 year and 0.561 were also estimated correspondingly. The annual total mortality (Z) was 1.03/year, natural mortality (M) was 0.635/year at mean annual surface water temperature of Indus River 21°C, fish...

Irshad Ahmad1,2*

... adopting the regulatory methods of domestic and international quarantine. This paper provides updated information about IPM, which look like a promising paradigm that can be adopted for the control of red palm weevil.

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Yuan Zhang1, Qingyue Han1, Peiquan Du1, Yi Lu1, Lianmei Hu1, Sadaqat Ali2, Khalid Mehmood2, Sajid Hameed2, Zhaoxin Tang1, Hui Zhang1* and Ying Li1*

 

...ntroduces the diagnostic methods of IHC and immunohistochemistry, rarely used in the diagnosis of lymphoma in clinical practice in China, which can provide a reference for veterinarians in clinical diagnosis.

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Yuan Zhang1, Qingyue Han1, Peiquan Du1, Yi Lu1, Lianmei Hu1, Sadaqat Ali2, Khalid Mehmood2, Sajid Hameed2, Zhaoxin Tang1, Hui Zhang1* and Ying Li1*

 

...ntroduces the diagnostic methods of IHC and immunohistochemistry, rarely used in the diagnosis of lymphoma in clinical practice in China, which can provide a reference for veterinarians in clinical diagnosis.

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Guangming Xiang1, Ran Di1, Yaowu Wang1, Shangquan Gan2, Shouren Liu2, Xiangyu Wang1, Wenping Hu1, Qiuyue Liu1* and Mingxing Chu1*

...respectively. Genotyping method was used to analyze genotypes among Altay sheep (fat-rumped breed) and White Suffolk (thin-tailed breed) by Sequenom MassArray. For PRKAG3 gene, a c.1744C>T SNP and a c.1840C>T SNP have been genotyped. For SREBF1 gene, an unknown synonymous c.2878A>G SNP was detected. The genotype distributions in those two loci were significantly different between fat tail and thin tail breeds by chi-square test (P<0.05).

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Neven Waheeb1*, Sherif Marouf2, Essam Nasr3, Shaymaa Abdelmalek2 

...nvasive and non-invasive methods. Invasive methods require endoscopy which is uncomfortable and unacceptable for most patients. Therefore, non-invasive methods particularly serological tests are easier and comfortable for patients. The objective of this study was to comparatively evaluate noninvasive enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR) in the diagn...

Syukriah Syukriah1, Ulinnuha Nur Faizah2, Hendry T.S. Saragih3*, Rizki Fitrawan Yuneldi4, Soenarwan Hery Poerwanto5, Raden Roro Upiek Ngesti Wibawaning Astuti5, Stephan Immenschuh6 

...ed by using the paraffin method. There were sigificant results in the parasites levels and the increase in P. berghei infection followed an increase of oxidative stress in mothers and offspring mouse in the treatment and control groups. The liver and spleen of mothers have been affected with P. berghei infection, however, there are still no effects on the offsprings, and the body weight of the offsprings from infected mothers were lower than uninfected mothers...
Indah Prihartini1*, Miftachi Ari2, Manar Fayiz Mousa Atoum3,4, Akhis Soleh Ismail1 and  Listiari Hendraningsih1
...litic TPG probiotic. The method used was Randomized Complete Block Design (RCBD) with four levels of treatments and three groups. In addition, a further test was conducted using Duncan’s multiple range due to a significant difference. The treatments were rice straw added by probiotic level which consisted of (P0: 0 %; P1: 0.5 %; P2: 1.0 %; P3: 1.5 %). Also, the in-vitro gas production was observed at 2 h, 4 h, 8 h, 16 h, 24 h, 36 h, 48 h, 72 h, an...

Lin Huang1, Ling Mai2, Keyan Zhong1 and Xinjun Chen3*

...modification sites. Both methods predicted that SeFABP contained 5 linear epitopes, and were highly coincident, indicating that SeFABP had good immunogenicity. Its secondary structure contains 2 α-helix (13.07%), 10 β-sheet (56.92%) and 30% random coli. The comparison of multiple FABPs sequences showed that the species were identical and highly conserved at multiple AA sites. Specifically, SeFABP has the highest similarity with T. multiceps and M. v...

Abdul Jabbar*, Anees-Ul-Hussnain Shah, Abdul Basit, Ghulam Ahmad, Aftab Ahmad Khan, Suleman Raza, Muhammad Sultan Ali Bazmi, Imtiaz Akram Khan Niazi and Ahmad Hussain

...roduction but the sowing method is a major one. A study to assess the consequence of different sowing methods on seed production of berseem (SB-11) was directed at Fodder Research Institute, Sargodha, Pakistan during 2017-18 and 2018-19. Different sowing methods, broadcast and row spacing of 15cm, 30cm, 45cm, 60cm apart were used. Plant height (cm), number of capsules/m2, number of seeds/c...

Lanjie Li1, Jingjing Zhang2, Ruiyan Zhang2, Ning Zhang2, Zixiang Wei2, Guiqin Liu1,*, Riaz Hussain Pasha3, Muhammad Akram Khan4 and Saif-Ur-Rehman5

...k of advanced extracting methodologies. In this study, an innovative extracting technology was established for the extraction of antioxidant oligopeptides from donkey meat by two-step enzymatic hydrolysis. The influence of enzymolysis on the degree of hydrolysis (DH) and protein recovery (PR) were evaluated, and the chemical composition, antioxidant activity, and molecular weight distribution of oligopeptides in hydrolysates were studied. The optimum enzymatic...
Syed Makhdoom Hussain1,*, Hina Gohar1, Muhammad Asrar1, Muhammad Mudassar Shahzad2, Azhar Rasul1, Majid Hussain3, Muhammad Zubair ul Hassan Arsalan1, Nisar Ahmad4 and Aqsa Sharif1
...etermined using standard methods and formulae. Highest minerals absorption (Ca, Na, K, Fe, Cu, P, Mg and Al) was observed in the fish fed at 400mg/kg of polyphenols in canola meal based diets. Similarly, best hematological parameters (RBCs, WBCs, PLT, Hb, PCV, MCHC, MCH and MCV) as well as proximate composition (crude protein, crude fat, ash, moisture and carbohydrates) were noted in fish group fed on 400mg/kg of polyphenols in diet. Hence supplementation of p...

Juliet N. Ozioko1, Obinna V. Ayogu2, Benjamin O. Ezema1, Dilibe C. Uramah3 and Kingsley O. Omeje*,1

...uantified using standard methods. Alkaloids, flavonoids, tannins, saponnins and terpernoids were detected. The leaf extract had 0.415 % of alkaloids, 0.102 mg/TAEq of tannins, 0.211 mg/QEq of flavonoids and 0.360 mg/GAEq of phenolics. Phytol, oleic acids, hexadecanoic acids and octadecanoic acids were detected in the leaf extract by Gas Chomatography-Mass Spectroscopy. White blood cells, hemoglobin and platelets were some of the hematological indices increased...

Shuangye Wang1,2, Yunlin Zhao1,2, Zhenggang Xu2,3,*, Junzhi Chen3, Guiyan Yang3, Song Wang2 and Kangkang Jiang2

 

...% minimum convex polygon method. A maximum flooded time index was calculated and the overlay analysis with seasonal home range data was employed. The results showed: (1) in seasons, the home range size of winter was the largest while it was the smallest in summer. It was also found that home range of dry and wet periods in 2017 was larger than in 2016 for the same period; (2) The milu population lived together in the released area. They even moved to outside o...

Raheela Naz1*, Muhammad Aftab1, Ghulam Sarwar2, Ana Aslam1, Qudsia Nazir1, Asifa Naz3, Abid Niaz4, Farah Rasheed1, Amina Kalsom1, Nisa Mukhtar1, Sadia Sultana1, Ifra Saleem1, Arfan ul Haq1, Muhammad Arif1, Aamer Sattar1, Sarfraz Hussain5 and Muhammad Adnan Rafique6

...yield of crops, time and method of fertilizers application are the most important factors. Some growth stages are very sensitive, at those stages of growth; the addition of fertilizers is more responsive than others. In order to nutrients to become available when the plant needs them and to make maximum benefits, fertilizers should be applied at the right time. In this experiment, impact of fertilizer applying methods for ni...

Alexander Tsybulsky1*, Tatyana Tabakaeva1, Anna Klimovich2, Michail Shchelkanov1, Eduard Kostetsky1, Maksim Aliev1, Anton Degtyarenko3 

...in cats. The fundamental methods for such pathologies diagnostics, along with haematological analysis, include the replicative and expressive activity of FeLV evaluation in the infected organism cells, innate immune factors for antivirals evaluation, interferon system (IFN) in particular, molecular guard factors for cell genome integrity evaluation (p53, etc.). Methods. In domestic cats with a previously established diagnosi...

Ghassan Zahid1, Sania Begum2, Sikandar Almani3, Sahir Hameed Khattak2, Rajesh Kumar Soothar4 and Shakeel Ahmed Soomro4*

...sistance is an effective method to control the spread of rust disease in cereal crops particularly in wheat. The experiment was conducted on fifty-two wheat lines comprised of forty-five double haploids of wheat along with seven wheat genotypes. Besides that, three positive checks and three SSR primers were also included in the study to check the durability i.e. Opata-85 Sr2/Lr27check, Pavon-76 (Yr-29/Lr-46) and Tukuru (Yr18/Lr34) respectively. The molecular m...

Neda Amani1, Mehrdad Shariati1, Rahim Ahmadi2,3*, Mokhtar Mokhtari1 and Saeed Khatamsaz1

...measured using MTT assay method. iNOS, MMP9, caspase-3, -8 and -9 expression level were evaluated by real-time PCR and the caspases activity was measured by ELYSA method. Flow cytometry was used to detect and quantify the level of apoptosis in cell lines. The data were statistically analyzed between groups using ANOVA and student’s t-test. Decreased viability was observed in AGS and HCT cells exposed to 0.1 and 1 mg/ml...

Saba Iqbal, Asmat Ullah*, Muhammad Luqman, Hafiz Muhammad Akram, Muhammad Kashif Munir and Nawal Zafar

...d broadcast (0.05 kg/m3) methods. Moreover cost benefit ratio of ridge (1:1.15) and bed (1:1.25) planting techniques was higher than drill (1:0.93) and broadcast (1:0.44) techniques. In conclusion, it is recommended that wheat may be planted on ridges and raised bed to have more yield, high water use efficiency and sound financial return to farming community.

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Aasma Younas*, Ijaz Iqbal and Atif Akbar

...mportant feature of this method is the properties of a design can be easily obtained from the sets of shifts instead of constructing the actual blocks of the design. This paper presents the results of a study in which the computer algorithm MATLAB has been used for the purpose of constructing Balanced Crossover design. This design is directly applied to agriculture, as its give an example of wheat to explain the concept. Crossover Designs have been constructed...

Muhammad Niamatullah1, Abdur Rehman1* and Raheel Saqib2

...nalysis as a statistical method was used to assess the impact of tube well irrigation on agriculture production, household annual income, poverty line, kinds of land, sources of irrigation and inputs used on land. The significant (P<0.05) impact of household annual income of farmers was observed on agriculture production. The significant (P<0.01) impact of household annual income of farmers had kept farming community above poverty line and the significan...

Amtul Jamil Sami*, Sehrish Bilal and Syeda Anum Zahra

...s of semen supply or the methods used for its introduction can give clues about these findings.

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Anjum Ara1, Usman Ali1, Muhammad Furqan2*, Zulfiqar Ali2
Muhammad Mudassar Shahzad3, Khawaja Basharat Ahmed4
Riaz Aziz Minhas4, Waqar Ahmed2, Zakir Hussain2 and Mian Aman Ullah2
...rvey, tracking, trapping methods were applied to collect data related to diversity, distribution, density and questionnaire method applied to assess threats. Analysis of data revealed that four freshwater turtle species including Indian flapshell turtle (Lissemys punctate), Indian narrow headed softshell turtle (Chitra indica) Indian softshell turtle (Nilssonia gangetica) and Crowned river turtle (Hardella thurjii) were iden...

Mukhlisi Mukhlisi1,*, Tri Sayektiningsih2 and Ishak Yassir1

...e employed a point count method by following transects. Observers walked constantly along transect and stopped every distance of ±200 m to record all sighted birds for 10-15 min. We found 89 bird species: 67 bird species were identified in hill karst forest and 33 bird species were recorded in coastal karst forests. Eleven bird species were found in both study sites. The score of diversity, species richness, and evenness indices of hill karst forests wa...

Riaz Shah1*, Shaikha Hilal Al Ismaili2, Sheikha S. Al-Siaby1, Amal Mohammed Al Nasiri2, Thuraiya Hafidh Al Maskari2, Jamal AlSabahi3 and Huda Al-Ruqaishi3

...ng conditions and drying methods of basil may change its essential oil quantity and composition. The aim of this study was to determine the chemical composition of essential oils extracted from commercially available fresh and dried organic or conventionally grown basil available in Oman. The essential oils from Omani and Indian fresh conventionally grown basil, fresh organic Belgian basil, dried organic Egyptian basil and dried conventionally grown UAE basil ...

Muhammad Tariq Mahmood1*, Muhammad Akhtar2, Kaiser Latif Cheema2, Abdul Ghaffar3, Imtiaz Ali4, Muhammad Jahanzaib Khalid2 and Zeshan Ali5

...structed by Ward’s method. On the basis of Euclidean distance it was observed that members of cluster 3 (G.P-110, G.P-111, G.P-115, G.P-116, G.P-121, G.P-125, G.P-138, G.P-139, G.P-150, G.P-151, G.P-164, G.P-165) and cluster 4 (G.P-112, G.P-126, G.P-140, G.P-152, G.P-166) were most diverse. Results also showed that the genotypes of cluster 3 were high yielding and genetically diverse as well. Therefore, they may be preferred while making selections for c...

Muhammad Tahir Jan1, Sarfraz Ali Shad2, Mushtaq Ahmad Saleem3 and Muhammad Binyameen2,*

...ing insect pest scouting methods to determine the potential damage and to apply control measures for key pest like Earias vittella in cotton. Field experiments were conducted to assess the distribution of E. vittella eggs within the cotton plant. Out of 240 plants inspected, E. vittella females laid 204 and 194 eggs on 40.9% and 36.9%of the plants in 2011 and 2012, respectively. Spearman Rank Correlation showed that a significant and positive relationship (ass...

Shumaila Nazir1, Iqbal Javed1, Muhammad Luqman2* and Azra3

Mohammed H. Galhoum1, Hamza M. Eed2, Essam S. Soliman1* 

...The conventional culture method revealed up to 83% sensitivity and 90% specificity while the molecular analysis revealed up to 100% sensitivity and 100% specificity for Salmonella detection. The study concluded that the high prevalence of the Salmonella with high resistance against 60% of the tested antibiotics reflects a serious problem with the hygienic and biosecurity measures taken in the poultry and slaughterhouses, as well the extensive use of the antibi...

Hafiz Adnan Ahmad1,2*, Muhammad Aslam2, Salman Gul2, Tariq Mehmood2 and Munawar Ali Munawar2

...geenan-induced paw edema method at 10 mg/kg dose. Among all derivatives, two compounds 4g and 4b displayed better anti-inflammation potential than standard drugs celecoxib and indomethacin. While the anti-inflammation profile of compounds 4h, 4e and 4f during in vivo screening was also comparable to celecoxib and indomethacin. The structure-activity relationship (SAR) was also discussed with the reference of substituent nature. Present results showed that newl...
Heba Mohamed Shaheen1, Ali Meawad Ahmed1*, Hosny Abdelltief Abdelrahman1, Rania Helmy Abohatab Abdou2, Aya Salama Mohamed Kamel1
...by simmering is the best method guarantee consumers almost safe of broiler chicken meat.
 

Xuelong Chen1, Honghai Wang2, Wenchao Li1, Weina Guo1 and Yanping Qi1*

... with both test time and method. Together, our results suggest that BHV-1 infections in yaks are becoming an increasingly widespread and serious concern. As such, it is essential that this prevalence continue to be monitored, and that effective regulatory efforts be implemented in order to prevent the continued spread of this disease.

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Semra Okur Gumusova1, Gul Fatma Yarim2, Cuneyt Tamer1,*, Ayris Salt2 and Gokhan Inat3

...y the spectrophotometric methods. Data obtained revealed higher MDA levels in IPNV infected cells than negative control medium (p<0.001). However, SOD and GPx levels in IPNV infected cells were lover than negative control cells medium (p<0.001). These results indicate that, cell damage in RTG-2 cell line, that is caused by IPNV is assosiated with oxidative stress.

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Jie He* and Ren Yang

...rding to Pringle’s method. Results showed that compared with propofol group and vitexin group, levels of serum ALT, AST and LDH in propofol + vitexin group were reduced (P < 0.05), apoptosis index was reduced (P < 0.05), Bcl-2 protein expression was increased (P < 0.05), Bax protein and caspase-3 protein expression was decreased (P < 0.05). In conclusion, propofol and vitexin could up-regulate expression of Bcl-2 protein, down-regulate expres...

Fatma Desouki Mohammed Abdallah

...an important statistical method called principle component analysis to extract new carcass trait components of Japanese quail from old variables. The idea of this method is that it forms a new variable (linear combinations of them) by reduction the dimension of the data for a large number of old variables. A total of 720 values of data were used to represent the variables under study for three different lines of Japanese qua...
Alshimaa A. Hassanien1*, Asmaa Osama Tolba2, Asmaa A. A. Hussein3, Walaa M. Elsherif4
...was done. Disk diffusion method was used for antimicrobial resistance detection, while the impact of casein and α-lactalbumin was evaluated by well diffusion method. Results indicated that 53 (35.5%) and 35 (38.04%) of meat products and food handlers were positive for S. aureus, respectively. High degree of genetic homology was described between meat and human isolates of S. aureus. These isolates possess high antimicr...

Bilal Jan1, Farida Anjum1*, Aasma Bibi1, Syed Roohul Hussain1, Abdul Ahad1, Azeem ud Deen1, Zia Ullah2 and Farrakh Mehboob3

...Pakistan. The analytical method was optimized for a total of nine different pesticides (Atrazine, parathion-methyl, chlorpyrifos, captan, α-endosulfan, dieldrin, β-endosulfan, endosulfan sulfate, and α-cypermethrin) of various chemical classes, using Agilent’s Intuvo-9000 GC-µECD system. A total of thirteen peach samples, ten from fruit orchards and three from fruit markets, belonging to five different varieties were collected and ...

Hazel Tamakan* and Huban Gocmen

... by conventional culture methods, automated identification system and PCR-RFLP. This is the first study to research the presence of MRSP in cats and dogs in Cyprus. The PCR-RFLP method based on the pta gene was confirmed to S. pseudintermedius. Methicillin resistance results were revealed with VITEK 2 and mecA gene detection was performed by PCR. A total of 100 samples including 50 cats and 50 dogs were examined from 6 veter...

Tarık Bugra Saruhan and Alptug Sarı*

...and indirect observation methods were used in field studies. Direct observations were made by one or two-person using point and line observations, as well as using camera traps. In indirect observations, signs such as the jackal’s footprints, scats, etc. were used. As a result of the research, data such as 253 camera trap images of the jackal, direct observation in one area, 16 jackal howling sounds in three areas, and footprints in one area were obtaine...

Hilarius Yosef Sikone1*, Budi Hartono2, Suyadi2, Bambang Ali Nugroho2 

...or Regency. The research method used was descriptive quantitative, which was carried out by a survey method. Determination of the sample using two methods: purposive sampling (applied to farmer respondents) and snowball sampling (to collect respondents from traders and butchers). Samples used in the study were 115 respondents consisting of 90 farmers, ten collectors, four inter-island catt...

Heba El-Zahar*, Zeinab Abd El-Rahman, Abbas El-Naggar 

...imple and cost-effective methods of diagnostic and clinical assessment are urgently required. The goal of this study was to look at the hematobiochemical alterations in association with the evaluation of C-reactive proteins (CRP), haptoglobin and fecal calprotectin concentration as prognostic markers in dogs with IBD. After a detailed clinical, laboratory and ultrasonographic examination 21 IBD dogs with symptoms of chronic gastrointestinal diseases were chose...

Naila Shahzadi1, Hafiz Muhammad Tahir1*, Shaukat Ali1, Muhammad Farooq Bhatti2, Azizullah1, Shafaat Yar Khan3, Abdul Khaliq4

...lated to sericulture and methods to improve economic and biological traits through diet supplementation have been discussed.
...

Evrim Sönmez

...ith dipping and spraying method. It was found that as the concentration of B. bassiana, which was applied at concentrations of 0.1%, 0.2% and 0.4% increased, the mortality rates on the adults of the pest increased and the number of eggs they laid and the number of adults emerging from bean seeds decreased. It can be said that different doses of B. bassiana are quite effective on the mortality rates of A. obtectus adults, the number of eggs they lay, and especi...
Tiara Widyaputri*, Aldryan Cristianto Pratama, Dian Vidiastuti, Yudit Oktanella
...D18 gene by the PCR-RFLP method is related to the BLAD carrier allele in Friesian Holstein crossbreed dairy cows. A total of 60 samples of cow’s whole blood were collected and extracted and DNA isolated. DNA amplification was carried out using primer Forward (BLAD_F) 5’-TCAACGTGACCTTCCGGAGG-3’ and Reverse (BLAD_R) 5’-CCCAGATTCTTGACGTTGAC-3’. Amplification of the CD18 gene segment on chromosome 1 exon 2 with a target length of 106b...

Ly Thi Thu Lan1, Nguyen Thi Anh Thu1, Lam Thai Hung2, Nguyen Thi Hong Nhan3, Le Thanh Phuong4, Nguyen Trong Ngu3* 

...ymorphisms, the PCR-RFLP method was used with the help of AcyI restriction enzyme. A polymorphic site was found in the population, with the frequencies of 11, 12, and 22 genotypes being 0.18, 0.67, and 0.15, respectively. At the end of the experiment, better body weight (965-976 g/bird) and daily weight gain (10.5-10.6 g/bird/day) were found in chickens with 11 and 12 genotypes. Carcass parameters were superior in chickens carrying these genotypes (P<0.05) ...

Muhammad F Tajol Ariffin1, Chai M Hian1, Muhammad Z Sukiman1, Mohd F Ghazali1, Siti M Zainal Ariffin2* 

...nd 72 h) using the ELISA method. The SCC was determined by the direct microscopic method. Results revealed a time-dependent change of Hp, SAA and AGP in the infected group compared to the control. The APP levels in the milk were significantly higher (p<0.05) than those in the serum. A significant good correlation between milk Hp concentration and SCC was observed (rs= 0.73, p<0.05). However, there were no associations ...

Nazakat Nawaz1, Nasir Mahmood Cheema2*, Malik Muhammad Yousaf3, Muhammad Jahanzaib1, Mubashir Ahmad Khan1 and Muhammad Munir4

...ction following pedigree method was continued up to F5 generation. The generations, F2 to F5 were raised for consecutive selection as single plant progeny rows along with parents. PG-1090 being medium duration showed overall higher mean yield (4912 kg ha-1) in preliminary, advance, national uniform yield and on-farms trials compared to other lines and check cultivars BARD-479 and Golden. Its attributes include 70% shelling, 100-kernel weight 66g, 20-pods lengt...

Ying Feng1 and Xuqun Pei2,*

... RT-PCR and Western Blot method were used to detect the expression of autophagy and apoptosis genes in neurons. We found that patients with ischemic stroke suffered from different degrees of impaired glucose tolerance, and the HbA1c index of patients with impaired glucose regulation and diabetes was higher than that of the normal glycemic group at the beginning and 3 months after the onset. The proportion of LAA and SAO in the two groups, other than the normal...

Ji Xu, Zhonghua Liu, Zhenhai Cui and Wenhai Zhao*

...ression level. The above methods were used to detect the effects of miR-99a overexpression on IL-1β-induced chondrocyte inflammatory factors and apoptosis rate. Anti-miR-NC and anti-miR-99a were transfected into chondrocytes, respectively, and treated with a culture solution containing GSTT and IL-1β for 24 h. Inflammatory factor levels and apoptotic rates were detected. After IL-1β treatment, the levels of inflammatory factors IL-6, TNF-α...

Sheng Dong1*, Ning Li2, Jiawei Zhang2 and Ting Wang2

...rvention for 24 h. CCK-8 method and colony-forming assay were taken to detect cell proliferation ability; flow cytometry was adopted to detect apoptosis rate; qRT-PCR method was used to detect the expression of KCNQ1OT1; Western blot method was used to detect Nephrin, GRP78, GRP94, and Caspase 12 protein expressions. We found that DS enhanced the proliferation of high glucose-induced podoc...

Husmaini1*, Riza Andesca Putra1, Indri Juliyarsi1, Tevina Edwin1, Linda Suhartati1, Aditya Alqamal Alianta1, Harmaini2 

...rs who kept the KBC. The method was survey method and purposive sampling to determine the respondents. The observed variables were breeder profile, maintenance management, number of KBC, actual population (Na), effective population (Ne), and inbreeding rate. The total population of KBC in Nagari Batu Bajanjang, Tigo Lurah District is 1960, with an actual population of 610 chickens, an effective population of 600 chickens, an...

Rana A. Ali1, Abd-Elraheim A. Elshater2, Heba A. Mohammed3*, Mahmoud Elshazly4 

Ayu Cusiayuni1, Risan Khaerani Nurfatahillah1, Rakhmad Perkasa Harahap2, Komang Gede Wiryawan3, Dwierra Evvyernie3, Anuraga Jayanegara3* 

...by using the mixed-model methodology. Results showed that the addition of lauric acid reduced methane production (P < 0.001). This decrease was followed by a tendency of methanogen population reduction (P < 0.10) and bacteria (P < 0.10) in the rumen. The addition of lauric acid did not alter total volatile fatty acids (VFA), acetate, propionate and butyrate, but tended to decrease ammonia concentration (P < 0.1). Lauric acid also reduced dry matter...

Rabia Bibi1, R. Muhammad Tariq2, Samir A.M. Abdelgaleil3 and Munawwer Rasheed1, 4*

...ing a Soxhlet extraction method with solvents of varying polarity (hexane, dichloromethane, and methanol). Five different concentrations of the extracts were subjected to the toxic assessment against two species of stored grain pests (Sitophilus oryzae and Callosobruchus maculatus). Results obtained from experimental trail proved that the C. maculatus were susceptible with higher adult mortality than S. oryzae. The highest toxic effect was induced by dichlorom...

Nuraini Nuraini*, Mirzah Mirzah, Yuliaty Shafan Nur, Harnentis Harnentis 

...udy used an experimental method with a Completely Randomized Design (CRD). The treatments were the types of fungi, namely Lentinus edodes, Pleurotus ostreatus, and Phanerochaete chrysosporium, and six replications. The parameter measured: cellulase activity, crude fiber, fiber digestibility, crude protein, and nitrogen retention. The results of phase 1 showed that the types of fungi had a very significant effect (P <0.01) on the nutrient quality of fermente...

Mashakgene Isaac Senoamadi, Thobela Louis, Tyasi Teedzai Chitura* 

...linical haemonchosis and methods of controlling Haemonchus contortus and other internal parasites in a communal farming system of Limpopo province, South Africa. Twenty-six sheep and one-hundred and sixty-three goats were evaluated for clinical haemonchosis using the FAMACHA© diagnostic system. Information on the methods of control used by the smallholder farmers was gathered through a questionnaire-based survey that wa...

Muhammad Idrees1, Bashir Ahmad2, Muhammad Waqas1, Syed Muhammad Mukarram Shah3 and Saad Ahmad Khan4

...fied using computational methods. In the ligand base pharmacophore, an already reported four ligands for the four proteins encoded by the resistant genes of M. tuberculosis were selected for the generation of pharmacophores. The validated pharmacophores model of all the four proteins, generated on the basis of ligand base, were selected for further screening of ZINC drug like database. As a screening results 486 structurally diverse hits for katG, 542 for PncA...

Djadouni Fatima* and Meliani Amina

...erent ancient and modern methods of treating these diseases and to share them with other societies.

...

Ahmed M. Darwish1*, Hassan R. Darwish1, Dalia M. Mabrouk1, Mohamed A. Abdelhafez1, Ahmed M. Abdel-Salam2, Ibrahim E. Mohamed3, Ibrahim M. Farag1 

...etermined by biochemical methods in all milk samples. Different genotypes of β-LG and LEP genes were detected by single-strand conformation polymorphism (SSCP-PCR), and then validated by sequence analysis. The results of SSCP-PCR showed a monomorphic pattern for the β-LG gene and a polymorphic pattern for the LEP gene. The sequence analysis showed that the β-LG gene has one genotype without any single nucleotide polymorphism (SNP) while the LEP ...

Kafeel Ahmad*, Muhammad Siqaf, Gul-E-Rana Abid, Ramla Somayya and Umama Qasim

...uction by disc diffusion method using the antibiotics cefotaxime (30 µg), ceftazidime (30 µg), cefepime (30 µg), amoxicillin-clavulanic acid (30 µg), imipenem (10 µg) and meropenem (10 µg). Three extended spectrum beta lactamases genes (blaCTX-M, blaSHV and blaOXA-10) and one metallo beta-lactamase gene (blaNDM-1) were analysed in the isolates. Eight (16%) isolates were positive for extended spectrum beta-lactamases producti...

Nahla Hamada Magd Khalil1*, Ihab Mahmoud Helal2, El-Desoky Hassan Ibrahim Dorrah1, Soad Ahmed Soliman Ismail3 

...y reduced in all cooking methods (100%). Heptachlor reduction by roasting was 86.36%, and completely reduced by microwaving and boiling. Heptachlor epoxide reduction was 94.88%, 79.49% after microwaving and roasting, respectively, and completely reduced by boiling. Concerning α-HCH, the reduction percentage was 95.83% for roasting, and 100% by microwaving and boiling while γ-HCH was completely reduced (100%) by all cooking ...

Aml M. Ragab1*, Maha R. Basyoni1, Enas A.I. Khoris2, Nadia A. Abd Elghany3 

... identified by molecular methods based on sequencing of genes, the potential of the groEL gene as a phylogenetic marker, and identified (hbl ,(nhe), (cytK), and (ces) enterotoxigenic genes. B. cereus isolates were analyzed for antibiotic susceptibility. A lab trial was conducted for two weeks using 60 Tilapia fish were divided into three equal groups, (1): kept as control negative, (2): infected intraperitoneally with (0.1ml) 8×107 (CFU/ ml/ fish) B. cer...

Nabila Khan1, Imran Ahmad2 and Muhammad Bilal Sadiq1*

...ized by response surface methodology (RSM). TPC and 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity were used as response variables. Almond hull extract was chemically characterized by Fourier transform infrared spectrometer (FTIR) and gas chromatography mass spectrometer (GCMS). The extraction conditions were optimized by using independent extraction parameters; sample to solvent ratio (1:20-1:40 w/v), solvent concentrations (20-80%, v/v) an...

Muhammad Ali Raza1*, Aneela Zameer Durrani1, Muhammad Hassan Saleem1, Kamran Ashraf2, Muhammad Muddassir Ali3, Kumayl Hassan Akhtar4 and Nazia Rubab5

.... Kit detection and HPLC methods were used to identify ABs residues. The samples were collected from Lahore. Raw milk of buffalo and cow collected from the open market have shown 23.5% as the highest positive and UHT milk as the lowest positive 8.5%. Thus, it is an indispensable question to be further explored prospectively. 
 
Novelty Statement | The detection of antibiotic residues including peni...

Uzochukwu Ephraim Emeto, Chukwuemeka Calistus Okolo* and Nwakaego Ernestina Nweze

... dentition and normogram methods respectively. The results showed that 89.9% [confidence interval (CI) 86.4%, 93.4%] of the horses harboured gastrointestinal endoparasites including strongyles (81.3%), Parascaris equorum (8.2%), Oxyuris equi (21.1%), Strongyloides westeri (13.5%), Anoplocephala spp. (10.2%) Paranoplocephala mamillana (3.6%). Polyparasitism was prevalent (43.4%, CI 37.8%, 49.0%). Associations existing between age groups or gender, and the prese...
Roy Hendroko Setyobudi1, Erkata Yandri2, Yogo Adi Nugroho3, Mardiana Sri Susanti4
Satriyo Krido Wahono5, Wahyu Widodo1, Lili Zalizar1, Elfi Anis Saati1, Maftuchah Maftuchah1
Manar Fayiz Mousa Atoum6, Muhannad Illayan Massadeh6, Dwi Yono3, Rangga Kala Mahaswa7
Herry Susanto2, Damat Damat1*, Dyah Roeswitawati1, Praptiningsih Gamawati Adinurani8 and 
Susi Mindarti1
...ing sugars (colorimetric method). Utilizing descriptive statistics, the research is presented in box-plot, with a t-test. It was recorded that Fe content in Mengani’s CCF [9.269 mg 100 g–1, ranged (4.717 to 15.686) mg 100 g–1] was lower than La Boite’s, although statistically insignificant (> 0.05). On five enhancers agents’ side, Mengani’s vitamin C [185.45 mg 100 g–1...

Muhammad Tahir Latif1*, Muzzammil Hussain1, Ayesha Latif2, Majid Hamid Bajwa1, Iftikhar Ahmad1, Ali Zohaib1, Naeem Faisal1 and Muhammad Hamza3

...non probability sampling method was used due to less adoption rate of MTR with a sample size of 240. Among targeted MTR growers 72% had 6-row riding type mechanical transplanters (MT), 18% had 4-row walk after type MT and 10% had 8-row riding type MT. Super Basmati and PK 386 were found more adopted varieties with 47% and 38% share of rice area. An 11-13% improved yield and 16-20% more net returns estimated with MTR by acquiring required plant population in th...

Jiang Wu*

...entified by conventional methods as Staphylococcus aureus, Salmonella, Escherichia coli and Listeria monocytogenes. Different bacteria were respectively inoculated into LB medium, the OD600 was adjusted to 0.5, and the initial bacterial concentration was adjusted to about 5×108 CFU/mL. T. officinale were dried in a hot air oven, and chemical substances in the dried powder were separated using Soxhlet extraction method ...

Maria Qibtia1, Sehrish Faryal1, Muhammad Wasim1, Farzana Chowdhary1, Muhammad Tayyab1, Ahmed Mansovuri2, Zeeshan Ahmed2, Muhammad Hamid3 and Ali Raza Awan1,*

... with the PCR-sequencing method. The statistical analysis was performed in SPSS using the method of chi-square test. All the SNPs were evaluated for the association of polymorphism. In the current study 20 out of 30 LP subjects were presented with the following SNPs: C/T-13910 (intron 13) and G/A-22018 (intron 9), representing a frequency level of 0.67 for association. Similar to this frequency of 0.22 and 0.44 was also foun...

Shuhuan Li1*, Yongheng Bo2, Youzhi Li3 and Xiuzhen Yang2

...LC), a simple but potent method was used to quantify atropine residues in beef. The results indicated that the method could measure atropine residues accurately, with the average recoveries between 82.37 and 88.31 % when the additional concentration ranged from 0.5 to 5.0 mg/kg, and a detection limit of 0.25 mg/kg, providing an effective and reliable method for the detection of atropine re...

Nkiru E. Ekechukwu, Felicia N. Ekeh, Chinenye M. Ohanu, Hope C. Ezinwa and Ifeanyi Oscar N. Aguzie*

...icking as the collection methods. Overall 12 species were recorded, Orba had the lowest species diversity with 7 species while Obollo-afor and Ikpa recorded 10 and 9 species respectively. Dipteran and Coleoptera were the most diverse and abundant order observed with Musca domestica (Diptera: Muscidae) being the most abundant species encountered. Equitability index of evenness revealed that Calliphora vomitoria, Blattella orientalis, Monomorium minimum, Dermest...

Muhammad Shaheen1* and Abdullah2

...rithm to accommodate the method for outlier detection on the basis of the threshold value. The threshold value of the outlier named as clus_span is computed by taking distance of each point from each other point and dividing it by the total number of points. All the points of a dataset that do not qualify the value of the minimum threshold are considered as outliers. New K Means with this add-in is tested on benchmark dataset for identification of outliers and...

Abdul Majeed1, Zhenlin Liang2, Lixin Zhu2, Chunli Liu2, Muhsan Ali Kalhoro1,* and Faisal Saeed1

...quency analysis (ELEFAN) method was used to estimate the VBGF parameters, which were L∞ = 39.9 cm (FL), K = 1.6 yr-1. However, total mortality (Z) was estimated using a length-converted catch curve analysis and found at Z = 5.31 yr-1 with a 95% confidential interval (CI = 4.77-5.84). Natural mortality was estimated at M = 2.16 yr-1 using an average temperature of sea 26○C in Pakistani waters, though, the fishing rate was obtained by F = Z-M = 3.15 yr-1...

Sher Bahadar Khan1,*, Mumtaz Ali Khan2, Irshad Ahmad3, Faheem Ahmad Khan4, Hamayun Khan1 and Sher Ali Khan5

...ics using disc diffusion method followed by detection of their respective antimicrobial resistant genes through PCR. Among them, the highest prevalence of Staph. aureus was found in Peshawar-Mardan division (30%), followed by Malakand (28.5%), Bannu-Dera Ismail khan division (25%) and Hazara division (16%). Over all the high resistance was found against Lin (96.25%) followed by AMX (82.5%), TET (63.75%), AMP (58.75%), SXT (50%), CHL (48.7%), CLR (36.25%), STR ...

Zhongxi Zhang1, Ping Zou2*, Jingfang Zhang1, Yujuan Zeng1 and Yujie Tang1

...were scored by Zea-Longa method. The effects of intravenous thrombolysis on brain injury and blood-brain barrier were detected by TTC staining. The expressions of ICAM-1 and MMP9 mRNA were detected by RT-PCR. The expression levels of ICAM-1 protein and MMP9 protein were detected by immunohistochemistry and Western blot. We found that compared with the model group, intravenous thrombolysis could significantly improve the behavioral disorder of rats, and signifi...

Feng Xu, Weijing Fan and Guobin Liu*

...3p expression. The CCK-8 method was used to detect the toxicity of rutin of different concentrations (0, 25, 50, 100, 200, 400 μmol/L) on human umbilical vein endothelial cells (HUVECs). HUVECs were treated with 100 mg/L ox-LDL for 24 h for establishing HUVECs injury model, and then treated with rutin in different doses. The cell activity and apoptosis rate of HUVECs were analyzed by CCK-8 method and flow cytometry, respe...
Tri Eko Susilorini1*, Ahmad Furqon2, Wike Andre Septian1, Desinta Wulandari3, Suyadi Suyadi3
...ment Length Polymorphism method. The result showed that three genotypes (CC, CT, and TT) and two alleles (C and T) were found. The frequencies of CC, CT, and TT genotypes were 0.450, 0.500, and 0.050 respectively, while the frequencies of C and T allele were 0.702 and 0.298, respectively. The different genotypes didn’t significantly affect milk production and compositions. Descriptively, Senduro goat with TT genotype had the highest milk production and c...

Aiman Batool1, Muhammad Sohail Sajid1,2*, Hafiz Muhammad Rizwan3**, Asif Iqbal4, Imaad Rashid5, Ibadullah Jan6, Faiza Bano7, Fiaz Ahmad8, Waqas Ahmad1, Muhammad Nisar Khan1 

...itative and quantitative methods, and Giemsa-stained thin smear, respectively. Morphological identification of ectoparasites was carried out under the stereomicroscope. The overall prevalence of GI parasites, haemoparasites and ectoparasites in the small ruminant population was 74%, 16% and 53%, respectively. Sheep were found more prone to endoparasites conversely ectoparasites were more prevalent in the goat population. A higher prevalence of GI, haemo and ec...
Ade Djulardi1*, Robi Amizar2, Tigor Sanjaya1
...udy used an experimental method with a completely randomized design (CRD) in a 3x2 factorial with four replications. Each replication consisted of 10 laying quails. The first factor is expired milk powder supplementation with three levels, namely 0%, 0.25%, and 0.50%. The second factor is the level of protein requirement with two levels, namely 20% and 18%. The variables observed were ration consumption (g/head/day), daily egg production (%), egg weight (g/gra...

Mimi Syazwani Jaapar1, Eric Lim Teik Chung1,2*, Nazri Nayan1, Mamat Hamidi Kamalludin1,2, Mookiah Saminathan3, Kalai Vaani Muniandy2, Muhammad Hazziq Mohd Hamdan1, Shokri Jusoh1, Faez Firdaus Abdullah Jesse2,4 

...according to established methods and data collected were subjected to one-way analysis of variance (ANOVA) and Duncan’s Multiple Range Test to determine the level of significance among treatments. The net gas production and gas production kinetics demonstrated notable changes (P < 0.05) among treatments. Besides, only the acetic acid and total VFA showed significant differences (P < 0.05) for the rumen fermentation characteristics. Generally, the T...

Hend K. Sorour, Mohammed A. M. Saleh, Azhar G. Shalaby* 

...nhibitory concentration) method 68.8% exhibited resistance. Colistin was recorded as 33.7% in chicken breast by HPLC. Furthermore, mcr-1gene was detected 54% using PCR. Sanger sequencing revealed the same identity (100%) between the three examined strains despite coming from different sources. It could be concluded that the practices inside poultry farms might be a possible source of spread of antimicrobial resistance to the food chain in Egypt.

Keywor...

Iram Liaqat1*, Umaima Bibi2, Muhammad Arshad3 and Najma Arshad2*

...etermination, Pyrogallol method, 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl radical scavenging assay, and free-radical-scavenging assay. Results revealed that O. vulgare and T. vulgaris possess high free radical scavenging capability and significant antioxidant activity. On the other hand, H. officinalis plant extracts exhibited low free radical scavenging ability and antioxidant potential. Obtained knowledge via present study have been extended further fo...

Junaid Ali1, Muhammad Nawaz2, Muhammad Ilyas3, Muhammad Umer Chattha1, Imran Khan1, Muhammad Bilal Chattha4*, Muhammad Arshad5, Muhammad Akram6, Mina Kharal7, Ehsan Ullah1, Muhammad Talha Aslam1, Fareeha Athar1, Ayesha Mustafa1 and Muhammad Umair Hassan1

...eral and protein. Sowing methods and irrigation application are an important agronomic consideration to get maximum production of lentil crop. Therefore, this study was conducted in RCBD with a two-factor split plot to determine the impact of various levels of irrigation and sowing methods on the growth and yield of lentil. Crop was sown by three sowing methods; flat, bed and ridge and wit...

Abdul Majid1*, Farah Naz1, Nasrullah Laghari1, Sanaullah Abbasi1, Sham Lal2 and Safdar Ujjan3

...MPC) through rehydration method. CDS identified that secondary structure was maintained in saline phosphate buffer (PBS) pH (6.4, 7.4 and 8.0), similar to that of water. Temperature based experiments depicted the resistance due to changes in the secondary structural conformation up to 50 °C, however such resistance was reversible after the cooling to 20 °C. In both solvents (PBS and water) lysozyme was predominantly folded to α-helical conformati...

Syeda Rubab Zaidi* and Safdar Ali Mirza

...ation and anion exchange methods were used along with protein estimation. The purified fraction was then subjected to enzymatic characterization to find its thermal stability, effect of pH and selected chemical compounds. Other significant experimental findings were; optimum incubation period was 7 days, inoculum size was 3 discs of 0.5 cm, CuSO4 as inducer improved production, laccase activity of crude extract i.e. 10.89U/ml increased to 15.07U/ml with purifi...

Ahmad Ali1, Zaib Ullah2, Muhammad Javaid Asad1*, Muhammad Rafi3, Muhammad Maqsood4 and Sajid Mahmood5,6

...ward in vivo through the method of way of the utilization of creature models. Photodynamic cure (PDT) is another creating method utilized for greatest malignancies treatment is fundamentally examined. We have planned troublesome and quick orderly tests to have an exam the viability of PDT. 
 
Novelty Statement | The study indicates the effect of Photosence Mediat...
Bulkaini*, Syamsuhaidi, Yusuf Sutaryono, Djoko Kisworo, Sukirno, Sukarne, Tapaul Rozi
... done by random sampling method. Research variables such as live weight, carcass weight and percentage, offal weight and percentage, edible offal weight and percentage, meat weight and percentage were analyzed using the Microsoft Excel program and regression correlation analysis. The results showed that carcasses with large size categories 77.67%, medium size 17.51% and small size 4.81%, while quality, which includes quality I, was 63.06%, quality II was 32.68...

Saima Mehar1 and Salma Javed2*

...sed through spectroscopy methods such as Fourier-transform infrared spectroscopy (FTIR), Ultraviolet–visible spectroscopy (UV), Mass spectrometry (MS), and Nuclear Magnetic Resonance (NMR). The structure of isolated chemical components employing bioassay-directed fractionation of both extracts. Concentration of the extract was directly proportional to the mortality of second stage juveniles, the resulted, six withanolides comprising coagulin E, G and J, ...

Imtiaz Ahmed*, Imran Khan and Zia Ud Din

...performed using standard methods. Folin-Ciocalteu, Aluminium chloride, and Colorimetric assays (ABTS and DPPH) were followed to determine total phenolic content, flavonoid contents and antioxidant status respectively along with“in-vitro starch digestibility”. The ash, fiber and protein content increased significantly while the carbohydrate content had significantly lower values (p, for all trends < 0.05) as the ratio of incorporation increased w...

Abdullah Channo1*, Asmatullah Kaka1, Qudratullah Kalwar2, Imdadullah Jamali3, Ghulam Jelani2, Muhammad Bakhsh4, Ghulam Nabi Dahri5 and Jai Parkash Goil6

...s rectal ultrasonography methods have been used. COD should be treated by using different treatment protocols such as hormonal, medicinal and homeopathic medicines.

...

Khalid Javed Iqbal1, Irfan Baboo2*, Arshad Javid3, Noor Khan4, Hamid Majeed5, Azra Anwer1, Zahid Farooq2, Muhammad Altaf6 and Moazam Jalees7

...the caudal vein puncture method into tubes containing anticoagulants EDTA and transferred immediately to the laboratory for the assessment of their blood profile. There was recorded no mortality after 96h of exposure period. IONPs significantly affected the counts of WBCs, Hb, MCH and MCHC of experimental and control groups. The analysis of blood profile demonstrated that haematological parameters; the number of WBCs and Hb were increased in T1 and decreased i...

Veronica Ngozi Emenuga1, Clara Idara Eleazar2*, Seto Tunrayo Aladenika1  

...rried out using standard methods. Antifungal biogram was performed. Result showed that all the cattle and the bedding tested positive for fungi, while 41(82%) of the milk samples had fungal contamination. Rate of isolation from cattle, bedding and the milk was not significantly (P= 0.365) different. The distributions of various species within the bedding was significantly different (P= 0.001). Trichophyton mentagrophytes had the highest rate in the beddings (...

Tinda Afriani*, Zaituni Udin, James Hellyward, Endang Purwati, Adisti Rastosari, Dwiki Wahyudi 

...min gradient and swim-up methods in Pesisir bull. The semen samples from three bulls were collected and evaluated for macroscopic and microscopic quality parameters including the percentage of motility, plasma membrane integrity (PMI) and acrosome intact (AI). The result revealed that the average motility, PMI and AI of sperms bearing X- and Y-chromosome in the albumin gradient method was 58.33±10.32% and 60.83&pl...

Abdul Ghaffar*, Niaz Hussain, Muhammad Nadeem, Khalid Hussain, Muhammad Aslam, Mudassar Khaliq, Muhammad Irshad, Zubeda Parveen and Muhammad Younas

...e the suitable selection method for genetic improvement in chickpea. The current investigation was conducted at the research area of the Arid Zone Research Institute, Bhakkar, Punjab, Pakistan. Twelve chickpea genotypes were sown in RCBD following three replicates. Data regarding primary & secondary per plant, days for maturity, plant height as well as potential yield were recorded. The measured data were analyzed to principal component analysis (PCA), cor...

Khalid Khan1*, Ahmed Farhan Saeed2, Sanam Wagma Khattak3 and Saima Liaqat4

...ltistage random sampling method was adopted to select the samples of 75 farmers from five tehsils of Lasbela namely Hub, Gadani, Uthal, Dureji, and Bela. Around 15 farmers were randomly selected and questioned in each of the tehsil. To achieve the objectives of study nonparametric correlation coefficient test and regression analysis are carried out. The study accentuated various factors which are responsible for the adaptation of poultry technologies and their...

Kusuma Adhianto*, Chairul Rahman Arif, Dian Kurniawati, Akhmad Dakhlan 

...This study used a survey method using primary and secondary data. Primary data was obtained from interview questionnaires and measuring the weaning weight of goats. Secondary data was obtained from weaning weight data in the recording. The observed variables were birth weight, weaning weight, weaning age, age of the dam at partus, type of birth, and sex. Results: The results of this study showed that the average weaning weights of male and female Saburai goats...

Lyudmila Yakovlevna Rodionova*, Irina Valeryevna Sobol, Lyudmila Vladimirovna Donchenko, Artem Vasilevich Stepovoy  and Andrey Georgievich Koshchaev

... standardized and modern methods of physical and chemical analysis of plant materials were used. The biochemical parameters of pumpkins were studied: the content of dry substances, sugars, titratable acidity, the content of ascorbic acid and carotene. Data on the content and fractional composition of pectin substances in different parts of pumpkins are presented. The kinetics of changes in the content of pectin substances in pumpkins after harvesting and stora...

Ayesha Khan*, Mohammad Tariq Aziz, Urooba Pervaiz and Muhammad Zafarullah Khan

...ple multi stage sampling method was used and respondents were selected from three villages namely Hajizai, Katozai and Batagram of tehsil Shabqadar. A sample of 112 respondents was drawn through proportional allocation technique based on 10% of total 1120 registered farmers. A pre-tested and well-developed interview schedule was used to collect primary data. Statistical Package for Social Sciences (SPSS) was used to analyze the data. Results indicated that 42%...

Zahin Anjum1*, Farhat Shehzad1, Shaista Ali1, Sumbla Yousaf2, Amina Rahat1 and Hamid Ullah Shah3

...fect of various grilling methods on PAH levels was evaluated. Mutton grilled with three grilling procedures, were analyzed for its PAHs content. A total of 120 samples were collected from local food vendor spots or general restaurants, and control samples of gas and electric grilled meat samples were also prepared and analyzed. Significant difference sp≤0.05 in PAHs levels were observed in different grilling procedures. Among the mutton samples, the highest...

Xiaoguang Su1,*, Yanjun Gao2, Yanling Wang1 and Yaohui Ma2

...-3p and EPHB2. The above method was used to detect cell viability and apoptosis rate after overexpression of miR-128-3p or inhibition of EPHB2. The dual luciferase report experiment verified the targeted binding relationship between miR-128-3p and EPHB2. Western blot was used to detect the expression of Ki-67, PCNA, Bcl-2 and Bax. Our findings showed that after HAE treatment, the cell viability was significantly reduced (P <0.05), the apoptosis rate was sig...

Liyun Chang1, Aiju Liu2, Jianshuang Zhang3, Yingbin Chen1 and Zhiyong Liu4*

...unosorbent assay (ELISA) method based on the SAG2 gene of Toxoplasma gondii (T. gondii) to improve the detection of toxoplasmosis in pet cats. Escherichia coli BL21 (DE3) was transformed with a prokaryotic expression vector, pET-21a-SAG2, which was constructed and induced to express a recombinant protein (SAG2), identified using SDS-PAGE and Western blot analysis. The purified protein was then used as a coating antigen to establish an indirect ELISA

Shambel Boki Dabi

...ssed using the titration method, while water turbidity, temperature, water pH, and dissolved oxygen were measured directly on site. A simple test kit was used to evaluate nitrite and ammonia levels. The result revealed a high level of alkalinity (66.3±39. 1mg. L-1) at downstream and lowest concentration (58±40mg.L-1) was found in the middle stream. The upper stream has a high CO2 concentration (14±3.6mg.L-1) while the middle stream has a l...

Ahmed A. Mohammed1, Madeha H. A. Darwish1, Eman A.A. Negm2, Ayman S. Abdel- Maguid3 

...andry. This reproduction method is relatively quick, which allows for faster turnover of the resources involved. Recent addition of Probiotics with broilers feed shows good efficacy to improve its production. So this expermintal study aimed to examine the influence of the addition of probiotics in broiler diets on the economic outcomes of production. Total 120 broiler chicks were divided into three groups of fourty birds each (C, L, & H) fed with diverse c...

Guang Yin, Wencheng Kong, Yuqiang Shan, Jian Zhang, Rongchao Ying and Sixin Zheng*

...re detected by the CCK-8 method and Transwell chamber experiment. Cell proliferation and apoptosis of NC-siRNA group, TINCR-siRNA group, cisplatin group and TINCR-siRNA+cisplatin group were checked by CCK-8 method and flow cytometry; and targeted binding relationship between TINCR and miR-646 was measured by dual luciferase reporter gene experiment. According to results of our study, compared with the control group there was...

Zhaojun Wang1, Yang Zhou2 and Xinghua Song3*

...ere detected using ELISA method before treatment and 3 months after treatment; incidence of adverse prognostic reactions was compared between the two groups. After treatment, the observation group had 34 markedly effective cases and 21 effective cases, with total effective rate of treatment at 91.67%. The figure was significantly higher compared to 80.00% of the control group, showing statistically significant difference (P<0.05). After treatment, the patie...

Agus Mulyono

...mage with classification method using ANFIS and determine the level of accuracy. In this study there are 3 stages, namely preprocessing, learning stages and testing stages. The results of the Java sparrow sex detection accuracy through texture and color analysis of beak images using the ANFIS method are 90%. This shows that texture and color can be used to detect the sex of Java sparrow

...
Muhammad Jalees
...iation. The survey-based methodology was used for the collection of data from the Telecom industry in Islamabad and Rawalpindi. 250 valid questionnaires was collected from different telecom industries. Data were analyzed using the Cronbach alpha reliability technique with the help of Statistical Package for Social Sciences (SPSS) version 23.0. Regression has been conducted to test the hypothesis. The result of the study confirms a significant relationship exis...

Jie Zhang, Na Li, Tingting Guo, Dandan Hu, Xiaofeng XU and Lili Zhang*

...ect of different feeding methods on the rumen bacterial flora of Tan sheep, a Chinese indigenous breed, using 16S rDNA high-throughput analysis. The rumen fluid was collected from 9-month-old Tan sheep that were either house-fed or grazed. The results showed that the diversity of rumen flora in the grazing group was significantly higher than that in the house-raised group (P < 0.01). In the grazing group, the abundance of 96 genera was significantly higher ...

Alexander Tsybulsky1*, Eduard Kostetsky1, Anton Degtyarenko2, Michail Shchelkanov1,2,3 

...se laboratory tests with methods for assessing the expression of interferon (IFN) genes can expand the possibilities of diagnosing animal cancers and improve the accuracy of differential diagnosis of benign and malignant neoplasms, in particular of the mammary gland (MG). Methods. Surgical material obtained after unilateral mastectomy with simultaneous hysterovariectomy, as well as peripheral blood samples of cats with MG tu...

Heba S.S. Salem1, Hend M. Megahed2, Marwa M. Sarhan2, Maha M. El Alem3, Gehan N. Alagmy3* 

... via traditional culture methods and molecular confirmation using PCR. All tested S. aureus isolates were positive for virulence-associated genes (nuc, icaA, and Hlg). In an experimental trial, a total of 75 healthy rabbits were divided equally into five groups (n =15/each group). The first group served as a negative control, the second group was injected intra-articulately with S. aureus, did not receive any treatment, and served as a positive control group,...

Desy Cahya Widianingrum1*, Himmatul Khasanah1, Melinda Erdya Krismaputri1, Susan Barbara Patricia Sembiring Meliala2 

... made by the non-heating method. The ration of the wafer consisted of 30% rice straw, 30% concentrate, 30% indigofera, 5% molasses, and 5% VCO. The group of treatments were wafer supplemented with VCO (P0); wafer supplemented with VCO saponification (P1); wafer supplemented with VCO protected by formaldehyde (P2); wafer without VCO (P3); and wafer supplemented with VCO mixed on indigofera (P4). Each treatments were using six replications and tested for their p...

Shailendra Sharma, Smriti Shrestha, Naresh Pandey and Laxman Khanal*

...he focal animal sampling method for 101 hours, and human disturbances were categorized into four levels based on the number and behavior of visitors. Out of the entire activity budget, resting (49.27%) constituted the highest proportion, followed by feeding (22.93%) and grooming (12.59%). There was a negative association between the grooming behavior and the number of visitors; however, no significant difference was observed in grooming proportion before and a...

Husmaini1*, Sabrina1, Firda Arlina1, Linda Suhartati1, Yozella Martilova2 

...Brown, 38 weeks old. The method used was an experiment with a completely randomized design (CRD) with seven treatments and three replications. The observed variables were ration consumption, ration conversion, egg mass production, hen day production (HDP), egg weight, shell thickness, and egg cholesterol. The results showed that supplementation of probiotics Lactococcus plantarum and Pediococcus pentasaceus using a purple sweet potato carrier at a dose of 2 gr...

Nuraini Nuraini*, Yuliaty Shafan Nur, Ade Djulardi, Robi Amizar, Yesi Chwenta Sari

...brio molitor. This study method was a completely randomized design (CRD) with six treatments and three replications. The treatment variations consist of commercial ration (CR) and tofu dregs (TD) were treatment P1 (100% CR), P2 (80% CR + 20% TD), P3 (60% CR+ 40% TD), P4 (40% CR + 20% TD), P5 (20% CR + 80% TD) and P6 (100% TD). The observed variables were fresh weight, crude protein, and crude fat of Tmc. The Phase 2 application to laying quails is the utilizat...

Shomaila Sikandar1*, Imran Afzal1 and Sadaf Sarfraz2

...rly mycotoxins detection methods and making regularity bodies to contain the spread of mycotoxins. This review summarizes the occurrence and toxicity of five major types of mycotoxins associated with food and feed and their importance in human nutrition and animal health.
...

Fengbin Jin1, Xin Liu2 and Yanqiu Yue3*

...he efficacy of these two methods was compared between two groups. Besides, we compared the changes in the vital sign (including blood pressure and heart rate), blood-gas indicators and scores of symptoms before and after treatment between two groups. Additionally, we also observed the adverse reactions of patients in two groups. After treatment, the effectiveness rate of patients in the observation group and control group were 96.49% and 79.70%, and the differ...

Sohail Nadeem1, Muhammad Aziz2*, Ayesha Mohy ud Din1 and Tayyaba Jamil1

...de through computational methods as well as to evaluate its antifungal properties. In order to achieve the goal, softwares like Avogadro, Gamess, Gabedit, Chimera and free online databases were used. The infrared and raman spectra were computed at the harmonic level with restricted Hartree-Fock in basis sets 3-21G and 6-31G. Molecular docking is calculated using online server with Lanosterol 14α-demethylase to know antifungal properties. The theoreticall...

Huajun Lin, Wei Qu and Zhuxian Yu*

...p by random number table method. Patients in the experimental group were treated with Dex and ganglioside, and the control group was treated with conventional methods. The clinical treatment effects, connective tissue growth factor (CTGF), TGF-β1 levels in serum and cognitive function were compared between the two groups. The total effective rate of treatment in the combination group was 93.75%, which was significantly ...

Indyah Aryani 1,3*, Suyadi Suyadi2, Hartutik Hartutik2, Kuswati Kuswati2 

...g whitlock sedimentation methods. The specific species was identified by their morphology. A total 43 Madura cattles were infected by nematode worm as follows: Oesophagostomum sp., Cooperia sp., Capilaria sp., Ostertagia sp, and Moniezia sp) while, 14 Madura cattles infected by trematode worms (Fasciola sp and Paramphistomum sp). The prevalence of gastrointestinal endoparasite was found as follows: Cooperia sp. 31%, Fasciola sp. 12%, Oesophagostomum sp. 6%, M...

Hafiz Qaisar Yasin1*, Dora Marinova2 and Muhammad Naveed Tahir3

Marcos E. Bollido1,2*, Renell Jay G. Villaluz1,2 and Ronald L. Orale1

...was examined using mixed methods. Supply and consumption were forecasted using Autoregressive Integrated Moving Average (ARIMA) models. Pork prices increased by 22-50% in 2021 compared to 2010 data. Live-weight pig prices also increased by 9-39% for the same year in review. It was noted that 27% of the supply was locally produced, and 73% were imported from other regions. Locally sourced pig supply is pegged at 53%, and the rest (47%) are imported from various...

H. A. Aidaros1*, S. S. Khalafallah1, M. S. Diab2, Nehal K. Alm Eldin2, Halla E.K. El Bahgy1 

... farms is the preferable method to reduce the risk of pathogenic bacteria and reduce the use of antibiotics.

Keywords | Poultry farms, Hygiene, Enterobacteriaceae, Antimicrobial Resistance, Resistance gens. 

...

Mai A Fadel1*, Ahmed M Elmahdy1, Jihan Mostafa Badr2, Mohammed AM Saleh3, Mona A.A. AbdelRahman3 

...id chromatography (HPLC) method was developed to quantify the concentrations of enrofloxacin and amoxicillin in serum and different tissues of chickens. The pharmacokinetics and pharmacodynamics indices of both antibiotics were calculated and statistically analyzed. Aspartate aminotransferase, alanine aminotransferase, urea, and creatinine serum level were also estimated. From our findings, we concluded that the usage of a half-dose combination of enrofloxacin...

Ejaz Ul Haq, Urooba Pervaiz*, Muhammad Zafarullah Khan and Ayesha Khan

...nology as it is the best method to grow off-season vegetables and for yields, both the field staff of extension department and farmers must be provided training for development of tunnels and package of production timely. Government should facilitate to establish agriculture insurance and provide subsidy to farmers (in energy, fertilizer, seed etc).

...

Nurdan Urvaylioglu1*, Ogunc Meral1, Serkan Sayiner2, Ulvi Reha Fidanci1 and Arif Altintas1

...lk may be an alternative method for diagnosing subclinical mastitis (SM) in dairy cows. Ten subclinical-, 16 clinical mastitis and 39 healthy Holstein cows were allocated to the study following California Mastitis Test (CMT). Thus, a total of 65 Holstein cows participated in the study. In the study, Staphylococcus aureus (n = 10) was isolated as the most dominant bacterial species seen in clinical and subclinical mastitis samples. Somatic cell counts (SCC) in ...
Alicja Kowalczyk1*, Inga Kowalewska-Łuczak2, Ewa Czerniawska-Piątkowska3*, Jesús Juan Cantalapiedra4, Joaquim Orlando Lima Cerqueira5,6 and José Pedro Pinto de Araújo5,7
...mined using the PCR-RFLP method. Evaluation of sperm quality in terms of concentration, volume, motility, and sperm viability was performed using photometry, flow cytometry, and a computer assisted sperm analyzer. The above studies indicate that all four nucleotide substitutions analyzed in exon 4 of AQP7 gene affect the individual quality characteristics of the analyzed semen of Holstein-Friesian bulls.

...

Farheen Shaikh*, Saima Naz and Nadir Ali Birmani

...e collected hand picking methods with fine brushes and preserved in 70-75% ethyl alcohol. The permanent microscopic slides with cover slips were prepared for final mounting in Canada balsam. At present 130 specimens were recovered and classified into 4 species which belongs to 3 genera. The species and their prevalence are 19.23% for Menopon gallinae (Linnaeus, 1758), 23.84% for Menacanthus stramineus (Nitzsh, 1818), and 22. 30% for Menacanthus abdominalis (Pi...

Shakeel Ahmad1*, Humaira Wasila2, Juweria Abid3, Nazir Muhammad4 and Hazrat Usman5 

...ample. AOAC 15th edition methods were used to analyze moisture, ash, carbohydrates, fats and protein contents of milk products. Atomic absorption spectroscopy and flame photometry were used for mineral analysis. Total phenolic compounds were evaluated by the Folin-Ciocalteu method. The aluminum chloride colorimetric method was used for evaluating total flavonoids contents. Antioxidant acti...

Kanwal Hanif1, Dilbar Hussain1, Qurban Ali1, Asad Aslam1*, Tamsila Nazir1, Muhammad Saleem1, Muhammad Faheem Akhtar1, Muhammad Zubair2, Najuf Awais Anjum3 and Muhammad Umar Qasim1

...surface residue bioassay method. The results revealed that new chemistry insecticides viz., Flubendiamide, Flonicamid and Chlorantraniliprole were comparatively safer to egg parasitiod followed by Chlorantraniliprole while Nitenpyram and Pyriproxyfen was found toxic to Trichogramma. Flubendiamide, Chlorantraniliprole and Flonicamidwas proved comparatively less toxic against T. chilonis with emergence percentage while in the case of survival of T. chiloniswasps...

Kokab Nazim1*, Asghari Bano1 and Ghulam Jellani2

...is recommended that this method can be employed to shortlist genotypes on cost effective basis.

...

Ghulam Qadar* and Khalid Nawab

...ur variables of teaching methodological competency were loaded on “job management” component which means that agriculture school teachers possessed high level of competency in job management. Likewise, Technical skill explained 18.05% variance has the second position in possessed competencies and job evaluation explained 11.44% variance, it has third position in possessed teaching methodological competencies. Sim...

Sehrish Akram1, Abida Butt1* and Shakil Ahmad Khan2

... treatment, leaf-dipping method was used and toxicity calculated after 48h exposure to the treatment. Both chemicals showed high toxicity against all instars of silkworm. However, results indicated that imidacloprid was more toxic for silkworm larvae compared to lead. The acute toxicity of imidacloprid formulation (IMF), imidacloprid technical grade (IMT) and Pb showed positive correlation with the concentration and negative with the age of larvae. From second...

Nuraini Nuraini*, Yuliaty Shafan Nur, Ade Djulardi, Robi Amizar, Yesi Chwenta Sari 

...uction (g). The research method in phase 2 used a completely randomized design (CRD) consisting of 5 treatments and four replications. The treatments were 0%, 2.5%, 5%, 7.5%, and 10% of Tenebrio molitor larvae in the diet. The variables observed were feed consumption, average daily weight gain, feed conversion, carcass percentage, abdominal fat percentage, and taste of meat carcass. Phase 1 demonstrated that different substrate compositions had significant eff...

Xibin Liu1,2,3, Weijun Guan2,* and Dong Zheng1,*

...the fibronectin adhesion method. The morphology of CPSCs was observed under an inverted microscope. Based on the growth curve test, we found that the growth mode of the cells was “S” type growth. The clonogenic assay was performed to determine the clonogenic efficiency CPSCs. The expression of cell surface markers was detected by immunofluorescence, flow cytometry, and RT-PCR, and it was found that the CPSCs positively expressed FGFR-3, collagen ty...

Jiahua Peng1,2, Shiyao Hua3* and Qian Wu4

...ere analyzed. The RT-PCR method was used to detect ovarian tissue TNF alpha and the level of IL-6 gene expression; Western blot was used to detect the changes in the expression levels of IKKβ and NF-κB protein expression. The results of fasting blood glucose and serum insulin test showed that DHEA could significantly reduce fasting blood glucose and insulin levels and improve insulin sensitivity index, and the effect was more obvious in the high-dos...
Farzana Bashir2, Aysha Saleem1, Khalid Iqbal Khichi2, Rubina Nelofer3* and Rauf Ahmad Khan2
...s using response surface methodology (RSM) based on three levels, three factorial Box Behnken Design (BBD). The significance of independent variables (flow rate, bacterial count and column height) and their interactions were tested by means the analysis of variance (ANOVA) with 95% confidence limits. The antibacterial susceptibility was optimized for flow rate, bacterial count and column height. High regression coefficient between the variable and response (R2...

Muhammad Said1, Amjad Hussain Mirani1, Abdul Kabir*1,2, Muhammad Haris Raza Farhan3, Abdul Latif Bhutto1, Ghulam Shabbir Barham1, Khaleeq Ur Rahman Bhutto4, Muhammad Uzair1, Maaz Khan1 

...primarily on the complex methods of mastitis detection, as identification of the etiological agents are vital to preventing mastitis in dairy cows.

Keywords | Mastitis, Diagnosis, Bovine Mastitis, Immunoassays, Diagnostic Techniques, Chronic Mastitis 

...

Ayesha Saeed1*, Farzana Kishwar2, Ahsan Nazir3, Sharjeel Abid4, Muhammad Abiodullah5, and Danial Ali6

... through electrospinning method, which was just wet before its application. Leaf extracts were used at three concentration levels (0.5, 1, and 1.5 %). Scanning Electron Microscope (SEM) images confirmed the formation of optimized nanofibers with diameter range from ±49 to ±545 nm. It was observed that addition of leaf extract increased the solution conductivity and decrease the surface tension and viscosity. Chemical reactions and presence of fun...

Rani Winardi Wulan Sari1, Novirman Jamarun2*, Suyitman3, Khasrad4, Elihasridas2, James Hellyward5, Gusri Yanti1 

...uction using an in vitro methodology. Samples of Hay Mangrove Leaves (HML) (Rhizophora apiculaca) and Native Grass (NG) were arranged into dietary tretmeants (Dry Matter (DM) Basic). The experimental design used is a randomized block design consisting of 4 treatments with 5 replications. The treatment was P1=60% Hay Mangrove Leaves (HML)+40% Native Grass (NG), P2=50% HML+50% NG, P3=40% HML+60% NG, and P4=30% HML+ 70% NG. Data were analyzed using General Linear...

Nishim Bhusal1, Bhuwan Raj Bhatt2*, Saroj Shrestha3 and Arjun Chapagain4

...amination (sedimentation method) to visualize eggs of Fasciola microscopically. The obtained data were coded and analysed using Microsoft Excel 2016. The overall prevalence of fasciolosis in cattle was found to be 15.56%. Age and sex-wise prevalence was found to be statistically significant (P<0.05), while stage and breed-wise prevalence was insignificant (P>0.05). Fasciolosis is prevalent moderately among cattle in Tillottoma municipality, which necessi...

Ishtiaq Ahmad1*, Muhammad Nafees1, Maryam2, Irfan Ashraf3, Ambreen Maqsood4 and Muhammad Saqib5 

...tion of different drying methods to improve shelf-life of dry mulberry fruits for their sustainable availability in the market for human consumption. For this purpose, common drying methods viz., sun drying, shade drying and oven drying were applied on freshly harvested black and white mulberry fruits to evaluate the significant drying method with minimal nutritional losses. The fruit samp...

Ansar Abbas1*, Muhammad Aman Ullah2, Abdul Waheed3 and Muhammad Asif4

...alli sheep by using both methods. However, the comparative analysis shows that the CHAID algorithm performed well as compared to the MLR (CHAID vs. MLR: R2 % = 79.38 vs. 73.27; Adj-R2 %= 78.70 vs. 72.50; SD ratio=0.45 vs. 0.51; RMSE= 3.15 vs. 3.58; RAE= 0.12 vs. 0.14; MAD= 2.41 vs. 2.74; CV%=13.28 vs. 15.11; AIC =638.50 vs. 708.28; r= 0.89 vs. 0.85). Consequently, it is hoped that the results of the study on the morphological characterization of Thalli sheep m...

Iqra Qazi1, Sohail Raza1*, Masood Rabbani1 and Muhammad Avais2

...d size, parity, breeding method and housing management was filled at sample collection time. The serum samples were processed by indirect ELISA to detect antibodies against BoHV-1. The overall sero-prevalence was 68.40%. Post pubertal animals (>3yrs) contributing more to disease occurrence. High sero-positivity was observed in buffalo (73.12%) as compared to cattle (60%) moreover, the sero-prevalence is higher in animals of greater size herds. Parity status...

Ghani Akbar*, Zafar Islam, Muhammad Umar and Shahi Salam

...onal flat basin flooding method

...

Neni Widaningsih1,2*, Budi Hartono2, Hari Dwi Utami2, Eni Siti Rohaeni3 

...Kalimantan Province. The methods used in this study are survey and observation methods. Data collection carried out includes; purposive sampling and accidental sampling techniques with the main respondents, namely breeders, collecting merchants, wholesalers, retailers, and consumers. The number of respondents in this study was 135 persons. Methods of data analysis carried out include; qual...

Iftikhar Alam1*, Sana Shah1, Muhammad Farooq2* and Samiya Rahman3

...nt study used the Delphi method to build consensus among selected (n=46) members of nutrition experts on research questions of interest. The experts were motivated to thoroughly review the postgraduate NEC contents against the three ‘domains’ (“Clinical Nutrition”, “Applied Nutrition” and “Basic Nutrition”), which served as standards for comparison. They were requested to answer on: “which subject matters f...

Fahrauk Faramayuda*, Soraya Riyanti and Sitty Mahanadhiandinie

...donesia using extraction methods other than brewing, i.e., maceration. Study of free radical scavenging activity of 1,1- Diphenyl-2- Picrylhydrazyl (DPPH) and the determination of the total polyphenol content of white tea leaves have been conducted using UV-Visible spectrophotometry. Results of the study revealed that the antioxidant activities of the ethanol extract, n-hexane fraction, the water fraction and the ethyl acetate fraction of white tea leaves had ...

Shama Sadaf1*, Komal Hassan1, Ayesha Saeed1 and Zeeshan Ahmad2 

... applied by pad dry cure method and finish was fixed by using of poly urethane binder. The presence of microorganisms was checked by ASTEM E2149 shake flask method before and after applying antimicrobial finish and after successive 25 washes. The results were analyzed through MANOVA. The fabric properties were checked by using ISO standard test methods. The fabric which was treated with A....

Ahmed M. El-Waziry1,2*, Saeid M. Basmaeil1, Ibrahim A. Alhidary1, Gamaleldin M. Suliman1,3, Mutassim M. Abdelrahman1, Maged A. Al-Garadi1 

...eed. Among the available methods of increasing production is using feed additives. One class of these additives is ionophores, a type of antibiotic that increases the nutritional value of the feed, thereby increasing production by reducing of feed intake, and decreasing the degradability of protein in the rumen, which increases its by-pass to the small intestine and decreasing the ammonia concentration in the rumen. Moreover, ionophores increase propionic acid...
Emel Hulya Yukseloglu1, Fatma Cavus Yonar1*, Omer Karatas1, Gulten Rayimoglu1, Faruk Asicioglu1, Elif Canpolat1, Onur Ozturk2 and Itir Erkan3
Ahmad Zaman, Azhar Rafique*, Farhat Jabeen and Tayyaba Sultana
...basis, using point count method for the collection of data. Naked eyes and binocular telescope (10x50) were used to count the birds and camera was used to photograph the birds. The study area was divided into two major landscapes types such as open field area (agricultural land, river Chenab sides, canals and cemeteries) and built up area (settlements, residential neighborhoods, and institutional grounds). We recorded 87 species of bird of 70 genera bel...

Marwa S. Khattab1*, Ahmed H. Osman1, Huda O. AbuBakr2, Rehab A. Azouz3, Asmaa A. Azouz4, Heba S. Farag5 

Eighty Mardiyan Kurniawati1*, Budi Santoso1, Fedik Abdul Rantam2, Widjiati3, Budi Iman Santoso4, Tri Hastono Setyo Hadi1, Gatut Hardianto1, Hari Paraton1  

Mochamad Rizal Umami1, Zainuri*2, Sebastiana Viphindrartin2 and Rafael Purtomo Somaji2

...yzed through descriptive methods and content analysis. The study found that the COVID-19 pandemic formed a new pattern of sugarcane trading system, switching from a contractual trade model to a free market model (redeemer). The model refutes the theory stating that the contractual model will be more efficient than the free market model due to its lower transaction costs. The proposition (substantive theory) produced in this study states that in conditions of s...

Sayed H. Al Habty1, Dina N.Ali2* 

...using the disc diffusion method; PCR was applied on (MDR) S. aureus isolates for discovering of blaz, mecA and VanA genes. 38% of yogurt samples had the highest prevalence of S.aureus followed by Damietta cheese (30%) and ice cream (14%). S.aureus isolates appeared high resistance to tetracycline, penicillin, oxacillin, ampicillin, streptomycin, amoxicillin/clavulanate and neomycin, in different percentages. blaz, mecA and VanA genes were detected at 60% for b...

Rahman Ullah1*, Muhammad Junaid2, Nabila Gulzar2, Rahat Ullah Khan3, Baseer Ahmad4, Ambrina Tariq5, Aamir Iqbal6, Mushtaq Ahmed7 and Mirwaise Khan8*

...ase chain reaction (PCR) method including other microbiological laboratory techniques (Total plate count, Gram staining, Hydrogen sulphide, Citrate, Urease and Indole Tests). A high level of total plate count (TPC) and E. coli was observed from both raw and pasteurized milk. The results also indicated a high incidence of ETEC i.e. 63.63% and 50% in raw and pasteurized milk, respectively. The most occurring enterotoxins are ST-I and ST-II (40%) followed by LT-I...

Muhammad Saif-ur Rehman1, Faiz-ul Hassan1* and Muhammad Sajjad Khan2

...ng unweighted pair group method of arithmetic means (UPGMA). Six hundred and four amplified bands were observed from successful amplifications of 73 primers (average 8.2 bands per prime). Only seven primers amplified no product or unidentifiable (smeared) bands. A total of 68 polymorphic bands were observed, showing 11.25 % polymorphism on average across cattle breeds. Results revealed a low genetic variation among the cattle breeds. A RAPD primer OPF-07 was a...

Talat Farid Ahmed1*, Muhammad Azeem Afzal1, Hashim Nisar Hashmi2, Hafiz Muhammad Yousuf2, Shamim-ul-Sibtain Shah1 and Muhammad Atiqullah Khan1 

...ed by the geo-electrical method of electrical resistivity survey (ERS) to mark out subsurface formations in terms of groundwater quality and quantity. The main focus was on identification of subsurface litho-logy and aquifer zones by resistivity method in parts of the Havelian Cantt, Abbottabad district, Khyber Pakhtunkhwa (KPK) Province and Sanjwal Cantt, Attock district, Punjab Province, Pakistan. Evaluation of the litholo...
Alia Tajdar1,3, Shaghef Ejaz2, Muhammad Shah Zaib1, Anum Ishfaq1, Syed Muhammad Zaka1*, Hafiz Muhammad Safeer1 and Khalid Abbas1
...ter harvesting different methods are used (cultural practices, mechanical practices, biological practices), but chemical control practices are dominant among all. Besides quick and effective outcomes of chemical control. It also has side effects (residues in fruits, human health issues, resistance, damage to non-target population, environment pollution). In the present study the effect of different botanical extracts on the olfactory response of B. zonata and ...

Ilhama G. Kerimova1*, Viktor A.Krivokhatsky2, Merve N. Aydemir3 and Lala N. Mamedova4

...able”. The genetic method has finally solved this question; now Palpares sp. questionable (IGK15) is surely assigned to P. turcicus. Myrmecaelurus solaris (Krivokhatsky, 2002) (IGK2) ML. and M. trigrammus (Pallas, 1781) (IGK22) differ from each other in dendrogram by more than 10%. The NJ tree shows that the genus Myrmecaelurus (Costa, 1855) is supported by 97-100%, and it is connected with another close genus Nohoveus (Navás, 1918) (Azerbaijanian...

Nazish Manzoor1*, Alim Khan1, Muhammad Yaseen1, Abid Ullah1, Zulqarnain2 and Iftikhar Jan2

Muhammad Yaseen1*, Muhammad Luqman1*, Bushra Pervaiz2, Rana Shahzad Noor3, Muhammad Ameen4, Sadia Hassan3 and Wassi Abbas1

...e face-to-face interview method. Descriptive statistics; mean, frequencies and percentages were applied to draw results and to interpret. The prominent mode of rural advisory services by the public sector was ‘training program’ whereas for the private sector it was ‘advice on phone’. The public and private sectors should consider diversifying their strategies for the provision of rural advisory services that target improvements in farm ...

Muhammad Azam1*, Shaista Naz1*, Malik Muhammad Shafi2, Muhammad Jamil Afridi3 and Ahmad Waheed4

...asis. The dietary intake method was used for the assessment of farm households’ food security by using of 2450 kcal/Day/AE. Descriptive statistics, binary logistic regression, frequencies, and percentages were the main-statistical techniques for data analysis. It has been found that majority of the farm households (65%) were food secure, still the issue of food insecurity prevailed among the 35% of the farm households. The positive determinants included ...

Umar M. Bello1*, Samuel A. Ojo1, Abdurrahman Ghaji1, Ambrose A. Voh (Jr)2, Muazu N. Bappah3, Casmir O. Igbokwe4  

...e reliable, non-invasive method for defining the reproductive phase and determining the optimum time of ovulation, fifteen (15) apparently normal, non-pregnant cycling Red Sokoto does were used to evaluate exfoliative vaginal cytology sequential pattern; as well as temperature (rectal and vaginal) changes relative to various stages of the oestrus cycle. The does were synchronized by treatment with two luteolytic dosages of PGF2α analogue (Lutalyse®,...

Adrial Adrial1*, Rudy Priyanto2, Salundik Salundik2, Ahmad Yani2, Luki Abdullah3 

...ince through observation method supported by laboratory analysis. Reproductive performance data were obtained through direct interviews and recording cards. Soil and forage samples were taken from the main forage source locations. A total of 138 blood samples were taken through the jugular vein from Bali cows and their crossbred cows with different physiological status. Soil and blood serum samples were analyzed in laboratory using Atomic Absorption Spectropho...

Amir Abdullah Khan and Muhammad Irfan*

...y using the streak plate method. Among them, one bacterium was found to be antagonist against the pathogen. This was identified as Bacillus subtilis 2i through 16SrRNA. The disease causing fungus was also isolated and identified as Schizophyllum sp. B2A through 18SrRNA.The strain Bacillus subtilis 2i was cultivated in sub-merged fermentation for the production of bioactive compounds. Various parameters were optimized to obtain maximum bioactive compounds produ...

Manzoor Ali Magsi1*, Naimatullah Laghari1, Ahmed Ali Tagar1, and Habibullah Magsi2

...e most efficient tillage methods for the wheat crop were shallow tillage + compost and deep tillage + NPK respectively. On the other hand, the highest calcium (Deep + FM), magnesium (Deep + Poultry, Shallow + FM), chloride (Deep + NPK and compost), and carbonate and bicarbonate (Deep + FM and Compost) treatments were found during the cropping year 2019-20 and 2020-21 experiments. Based on experimental results under wheat crop, application of reduced tillage tr...

Shahid Hameed Khan Khalil1*, Abdus Subhan3, Ghani Akbar1, Muhammad Asif1, Zafar Islam1, Adnan Shakeel1 and Fawad Anwar2 

... conventional irrigation methods, irrigation was used to maintain field capacity using 33kPa tension-meter reading during tillering, stem elongation, tillering and grain formation stages. It is clear from the experiment results that parameter of wheat growth including plant height, days to harvest, and number of tillers per plant and grain weight per ear were significantly changed by the application of different amounts of water (treatment) during the two year...
Huma Abbas1*, Nazir Javed1, Muhammad Kamran2, Sajid Aleem Khan1, Abdul Jabbar1, Akhtar Hameed1, Hira Abbas3, Azhar Iqbal2 and Ehetisham-ul-Haq2
...s. Different application methods regarding protective and curative were used to test the efficacy of Cartap, Virtako, Cure and Azadirachtin. Results revealed that Protective application of bio and synthetic chemicals was more effective at nematode suppression rather than being curative. Then the impact of bio and synthetic chemicals were tested in combination with NPK on nematode reproduction. Following treatments i.e. Virtako + NPK, Cartap + NPK, Cure + NPK, ...

Mohamed M. Bahr1, Mohamed S. Amer1*, Khaled Abo-EL-Sooud2, Shaimaa M. Kamel3, Marwa A. Fouly4, Ahmed N. Abdallah5, Ashraf A. Shamaa1, Omar S. El-Tookhy1 

Müge Aliye Hekimoglu and Sırma Sönmez

...rate. The image analysis method was used to determine skin colour. The chemical analysis method was used to determine the activity of enzymes. The results showed that supplementation of 2000 mg/kg of dietary significantly increased weight, length and specific growth rate (P < 0.05). While no change in colour was observed in the control group at the end of the experiment, it was determined that carnitine could have a 27% e...

Jin-Ming Zhao1,2, Yun Fang2 and Yue-Hua Sun2*

...se using the weight loss method, which has been found to be easily been conducted and also has a high accuracy compared with other methods, such as egg candling and floating. Our results confirmed the conveniency and accuracy of the weight loss method in estimating nest ages of Chinese grouse. We further discussed some cautions when applying the weight loss method<...

R. Hadadfar1, E. Mahdikhani-Moghadam2†, S. Baghaee2 and M. S. Bajestani1

...extracted by centrifugal methods. Psilenchus species were identified on morphological and morphometrical characters based on recent valid keys. Seven species from Psilenchus genus were identified viz., Psilenchus curcumerus, P. aestuarius, P. iranicus, P. hilarus, P. bilineatus, P. pratensis and P. bahiablancae of which P. bilineatus, P. pratensis and P. bahiablancae are new reports of these species for Iranian nematode fauna.

...

M. Israr1†, M. Habib1 and K. Nasira2

...;s sieving and decanting method (Cobb, 1918) followed by modified Baermann technique (Baermann, 1917). After processing, the nematode genera and species were identified (Siddiqi, 2000). Tylenchorhynchus usmanensis is reported from the first time from Pakistan is briefly redescribed and illustrated.

...

A. M. Rahoo1, 2, †, T. Mukhtar3, B. A. Bughio4, S. R. Gowen1 and R. K. Rahoo5

...d
extraction methods was investigated on the production of IJ in Galleria mellonella. There was significantly greater
emergence of IJ of S. affine from the Galleria mellonella using White traps than with modified Baermann extraction
trays. In Baermann extraction trays, the maximum emergence of IJ of S. affine was observed at an inoculation dose
of 400 IJ followed by the dose of 50 IJ....

M. A. Radwan†, M. M. Abu-Elamayem, S. A. A. Farrag and N. S. Ahmed

...
application methods significantly reduced tomato root galls and 2nd stage juvenile numbers in soil compared with
control. Except DNSA and AA, foliar application of tested organic acids was more effective in reducing nematode
galls than soil drench application. Foliar application of ASA caused superior effect in the reduction of J2 in soil (100
%) followed by AA (89.44 %), OA (88.25 %) and SA (70.69 %). CA (96.52...

Nadia A Eltablawy1, Ibrahim El Tantawy El Sayed2, Hamed Mohamed Abdel Barry2, Marwa A. Ibrahim3*, Maha Nageib Ahmed Serag ElDein2 

Istkhar† and A. K. Chaubey†
...
neighbour joining methods and comparative account on nucleotides analyses based on ITSs study confirmed the
present specimens as isolates of S. abbasi. The nucleotide composition resemblance within 5.8S gene with other
Steinernema species evident the less polymorphism and highly conserved nature of this region as compared to ITS1
and ITS2 and delimit the relationship of steinernematid nematodes.
...

A. Sattar1, †, A. Khan2, N. Khatoon1 and A. Mujahid3

...asitological
methods. The nematode parasites namely; Raphidascaris acus (Bloch, 1779) larvae, Metabronema magnum (Taylor,
1925), Haplonema immutatum (Ward et Magath, 1917) and Hedruris bryttosi (Yamaguti, 1935) were recorded from
the gut of the catfish, Arius arius (Hamilton, 1822). These are new host records as these parasites have not been
reported from Arius arius species in the region, off the Karachi coast.<...

F. Shahina† and G. Mehreen

... by using four different methods i.e.,
Maximum Evolution (ME), Maximum Likelihood (ML), Maximum Parsimony (MP) and Neighbor Joining (NJ)
based on the two makers. Sequence composition and phylogenetic analyses of these isolates showed closeness with
Steinernema  abbasi. On the basis of ITS rDNA region these seven Pakistani isolates were compared with seven
worldwide isolates of S. abbasi and species of bicorn...

F. Shahina† and G. Mehreen

...cted using two different methods maximum parsimony (MP) and
Bayesian inference (BI) in which most of them form highly to moderately supported clades.
...

Belema Robert1, Favour Welenya1, Deborah Achi1, Cynthia Onyeagwara1, Soala Obie Minimah2, Ebele Anulika Obichi2, Chidinma Charity Amuzie1* 

Rinat Islamov*, Dinara Turegeldieva, Altyn Rysbekova, Kuralay Sarmantayeva, Victor Semenuyk 

... were selected. The main methods of testing biological samples from laboratory mice and rats were realtime polymerase chain reaction and enzyme-linked immunosorbent assay. A high prevalence of some pathogens among conventional laboratory animals used by some academic institutes and universities of Almaty has been shown. Rodentibacter pneumotropicus has been detected in the SPF animals, while no other pathogens from the FELASA list have been detected. The obtai...

Salman Hussain1, Basit Zeshan2*, Rabiya Arshad1, Saba Kabir1 and Naveed Ahmed3

...es. Congo red agar (CRA) method was used to assess the ability of isolates to form biofilms. The results of the study showed that the prevalence of MRSA was 48%. The MRSA isolates were highly resistant (100%) to penicillin, β lactamase inhibitors, cephalosporins, and macrolides. All the MRSA isolates were susceptible to vancomycin antibiotic drugs. Cefoxitin (30 µg) disk diffusion test showed 100% sensitivity and specificity for the identification o...

Ahmad Abrar Khan1*, Ghulam Qadar1, Asif Ali Abro2 and Muhammad Awais3

...cases relied on chemical method for insects/pests control while one-third on chemical and physical both. The highest yield recorded was above 16000 Kg/acre achieved by only less than a quarter (23.1%), while collectively above fifty percent (53.9%), tomato growers were incompetent to achieve per acre yield of 12000 Kg. Majority (81.5%) tomato growers faced aphids attack while more than 50% reported whitefly, Cutworm, mites and Armyworm. Leaf miner, thrips and ...

Saima Mumtaz1,3*, M. Imran Kasana1, Riaz Alam1, Noorullah Khan1, Muhammad Noman1, Sanjeela Sabahat1 and Hussain Shah2

...tock and labor intensive method. To address these issues an alternative technique has been employed for quick multiplication of litchi. In the present study we have investigated the influence of various levels of Indole Butyric Acid (IBA) on four Litchi cultivars (Gola, Surahi, China and Bedana) for root induction, its development and success rate of survival in hardwood cuttings. Six treatments of IBA (1000, 2000, 3000, 4000, 5000, 6000ppm) and one with contr...

Dina N. Ali1*, Sayed H. Elhabtey2*, Manal M. Amin1  

...sing agar well diffusion method. The minimum inhibitory concentration (MIC) of MO and Nano-MO was 3% and 2%, on Listerea monocytogenes, Staphylococcus aureus, Salmonella typhi and E. coli bacteria, respectively. While, in inoculation of MO and Nano-MO in cheese complete reduction of examined bacteria was recorded at 3rd week of storage except for L. monocytogenes Nano-MO killed it at 2nd week. Overall acceptability (OAA) investigations showed better results of...
Aulia Evi Susanti1*, Rudy Priyanto2, Muladno2, Dewi Apri Astuti3, Lucia Cyrilla2
...lected through interview methods to obtain data on production systems and business models, reproductive and economic parameters, and feed applications to obtain production parameters at the Field Station-School for Smallholder Community (FS-SSC) Maju Bersama, Musi Banyuasin Regency in 2021. The feed application experimental design used a factorial 3x2 Randomized Block Design (RBD) with 10 replications. A descriptive analysis was conducted to evaluate the corpo...
Makruf Arif1*, Claude Mona Airin1, Dwi Sunu Datrianto2, Dinda Anggun Roro Sejati3
... eosin-negrosin staining method, and membrane integrity using the hypoosmotic swelling test (HOST) at the fresh semen, 5th, 24th, and 48th hours post-cold storage. The results revealed that in the 5th hour, percentage of motility, viability, and membrane integrity were not significantly different. However, at the 24th and 48th-hour results revealed significant differences by adding honey (P < 0,05). The 0.4% honey supplementation was the best to maintain KU...
Saleh Aljubran, Shaker Al-Suwaiegh, Yousef Alyousef, Sulaiman Alhajri, Mohammed Alghareeb, Abd El-Nasser Ahmed Mohammed*
... for semen deposition or method of embryo transfer. Therefore, this review was conducted to collect and consolidate the current knowledge of assisted reproductive techniques of males and females in addition to their importance for oocyte maturation, artificial oocyte reprogramming, embryo development and their resulting newborns.
 
Keywords | In vitro, Oocyte, Embryo production, Embryo transfer, Micromanipulation
...

Sutawi*, Ahmad Wahyudi, Abdul Malik, Suyatno, Asmah Hidayati, Imbang D. Rahayu, Endang S. Hartatie

...condary data exploration methods. There are three factors that can be attributed to the re-emergence of FMD outbreaks in Indonesia. First, the loss of the principle of maximum security. Indonesia’s success in freeing itself from FMD after 100 years is thanks to the implementation of maximum security policy by enacting a country-based system, which is to only import livestock and livestock products into the country from FMD-free countries. Second, the sha...
Aulia Puspita Anugra Yekti1, Sri Rahayu3, Gatot Ciptadi2, Trinil Susilawati2*
... Gradient Centrifugation method. The quality observed included individual motility, viability, abnormalities, concentration, total motile spermatozoa, chromatin damage, intact acrosome hood of spermatozoa, and sperm capacitation. The treatments consisted of T1 = unsexed semen, T2 = sexed semen X-bearing sperm, and T3 = sexed semen Y-bearing sperm. A general linear model with three treatments and ten replications was performed in this research. The data were an...
Fatma Salih Mohammed1*, Nasser Khedr1, Tahia Ahmed1, Liza S. Mohammed2
...justify;">The processing method used to create the meal affects the nutritional value of soybeans for birds. The most popular approach is solvent-extracted soybean meal (SE-SBM). However, extruded-expelled soybean meal (EE-SBM) is mainly utilized in organic poultry feed. This study aimed to investigate the effect of adding EE-SBM to broiler chicken Ross® 308 feed formulations on carcass traits and histomorphological changes in the intestine. A total of 198...

Muhammad Humayun Kabir* and Md. Saiful Islam

...ive and random) sampling method was applied to select the study location as well as the sampling unit (farmer/household head). Data were collected from randomly selected 336 farmers of Dhamrai Upazila (sub-district) under Dhaka district, Bangladesh using a face-to-face interview survey. Focus Group Discussions (FGDs) were also conducted to cross-verify the collected data. The data were analyzed using some common descriptive statistical parameters and an indepe...

Syed Shah Mohioudin Gillani1, Muhammad Naveed Tahir1, Adeel Anwar1*, Syed Ijaz Ul Haq2, Muhammad Awais3, Mujahid Iqbal4, Javed Iqbal5, Hina Ahmed Malik3, Syed Muhammad Zaigham Abbas Naqvi6, Raza Ullah7 and Muhammad Abdullah Khan1

... through non-destructive methods enable us to understand the different environmental impacts and help to respond to them precisely and rapidly. Chlorophyll pigmentation in plants is a supreme factor to assess the health of plants and ultimately leads to crop yield estimation. The present experiment evaluated the capacity of high-resolution satellite imagery to estimate wheat chlorophyll content coupled with ground-based information for accurately monitoring cr...

Saba Iqbal1, Salman Khurshid1*, Hafiza Mehwish Iqbal1, Qurrat-Ul-Ain Akbar1, Aqeel Ahmed Siddique3, Saqib Arif1, Shahid Yousaf2, Masooma Munir4, Abdul Karim Khan4, Shazia Arif5,  Abdul Ahad6 and Muhammad Arif7

...opy (NIRS) an analytical method in the field of laboratory instrumentation. The attempts have been made to explore the utility of NIR for variety of samples matrices. The present work has been undertaken to assess the efficacy of NIRS method for quantitative characterization of wheat grains in terms of hardness. Since wheat is the staple food and its hardness is one of the basic quality traits being used in local and interna...

M.M. Mikailov1*, O. Yu. Chernykh2, E.A. Yanikova1, G.A. Nurlygayanova3, O.D. Sklyarov4 

...f most other serological methods. Data from comparative serological studies of cattle blood serum in RNGA, ELISA, RA, RSK, RBP and RID with O-PS, confirmed the specificity of the tests and high sensitivity of RNGA with brucellosis erythrocyte antigen 

...
Safika1*, Ni Luh Putu Ika Mayasari1, Juliadi Ramadhan2
...rby-Bauer disk diffusion method was used to test positive isolates, followed by PCR method to discover the resistance coding genes. The results showed that ampicillin showed 76.0% resistance to Klebsiella pneumoniae, followed by oxytetracycline (72.0%) and tetracycline (68.0%), enrofloxacin (52.0%), and gentamicin (44.0%). All Klebsiella pneumoniae isolates carried the blaTEM gene, 57.20 % contained the tetA gene, 30.40 % co...

Trisiwi Wahyu Widayati1*, Aris Triyono Syahputra2, Andoyo Supriyantono1, Onesimus Yoku1, Deny Anjelus Iyai1, Priyo Sambodo1, Iriani Sumpe1 

... examination with native method, McMaster and sedimentation. Quantitative data are presented in mean ± standard deviation. The results of the comparison of the two systems were analyzed using the t-test. All pigs were superinfected by Ascaris spp, Trichuris spp and Strongyloides spp parasites with a mean EPG of 2185.56. The prevalence of parasitic eggs of Ascaris spp, Trichuris spp and Strongyloides in water, vegetables and soil ranged from 62.96% to 98...

Hasnain Raza1,2, Qun Liu1*, Muhammad Tariq Hanif2 and Yanan Han1

... Catch-based Monte Carlo method of estimating MSY (CMSY) and the Bayesian state-space Schaefer production model (BSM) based on the catch and effort time series data from (1993-2017) from the Western Indian Ocean (WIO), Pakistan. The five major fish species of commercial importance exploited by heterogeneous fishing fleets in the Exclusive Economic Zones (EEZs) of Pakistan have been undertaken as a case study for the stock evaluation. Altogether, both the model...

Lihang Wang, Qiling Chen, Tingsheng Lu, Shudan Yao, Xingwei Pu and Chunshan Luo*

...f modified Allen’s method was for injury of the spinal cord (T10) in rats and treated SCI was with MH or/and MPS immediately. Conduction of Basso-Beattie-Bresnahan (BBB) scores was on all rats at different time points after surgery. Subsequently harvest of the serum and the spinal cord tissues was behind euthanasia of rats. Detection of biochemical indicators, observation of cell apoptosis, and determination of Bax, Bcl-2, p38 MAPK and Smad2 were clarifi...
Hafiz Abdullah Shakir1*, Iqra Javed1, Muhammad Irfan2*, Shaukat Ali3, Muhammad Khan1, Farah Rauf Shakoori1, Javed Iqbal Qazi1 and Muhammad Abrar Yousaf1
...sign of response surface methodology. Tannase characterization data revealed that 5.0 pH, 30°C temperature, 60 minutes incubation and 0.45% of substrate concentration showed highest tannase activity. The results depict utilization potential of low cost substrate (corn leaves) to reduce the production cost of tannase.

...

Songruo Tao1, Cuiyi Liao1, Jinju Peng1, Yuexia Ding1,* and Yi Ma1,2,*

...hain reaction (ERIC-PCR) method. The results showed that the number of Operational Taxonomic Units (OTUs) types decreased with the increase of florfenicol concentration, and the percentage of OTUs number to bacteria were the lowest at 100 mg·kg-1 florfenicol concentration after 21d treating, which was 8.33%. The phosphorus-solubilizing bacteria were amplified by ERIC-PCR after 21d treating, the fingerprint type of ERIC-PCR decreased with the increase of...

Dalia Zaafar1*, Heba M.A Khalil2, Soha Hassanin3, Mohamed R. Mousa4, Mona G. Khalil1 

Noor Rahmawati, Alfi Rumidatul* and Sopandi Sunarya

...ntioxidant activity. The method used in this study was to ferment endophytic fungi from gall rust sengon in different media, namely PDB, PDB plus sengon wood powder, and PDB plus yeast, for 9 days, followed by extraction using ethyl acetate solvent and testing of antioxidant and antibacterial activity. The DPPH radical was used to measure antioxidant activity, and Bacillus subtillis was used as a representative for Gram-positive and Gram-negative For testing o...

Ghulam Jelani1, Muhammad Haris Raza Farhan2, Rida Asrar3, Muhammad Ahmad1,4*, Chanda Naseem5, Zafar Ali Khoso6, Majid Hussain Soomro7* 

...isolation and diagnostic methods as well as improper use of antimicrobial therapy causes the treatment failure. Different types of antibiotics are used to treat it and different research use antibiotics of aminoglycoside group. For preventing the flock from infection separates the infected birds from healthy ones. Different treatments show average growth and good performance with the recovery of clinical signs, histopathological changes, mortality, and morbidi...

Saleem Hussain1, Muhammad Tayyab1, Tauqir Anwar1, Talha Nazir2*, Muhammad Zeeshan Majeed3, Zohaib Asad2,4, Muhammad Adnan3 and Tajwar Alam5

...ions. Using foliar spray method, toxicity bioassay was conducted with three different concentrations (i.e. 250, 500 and 1000 ppm) of methanolic plant extracts under controlled laboratory conditions, while three different concentrations (25, 50 and 75 g/L) of each botanical extract was tested in the field trial. Results showed a significant mortality and suppression of S. graminum individuals at 72 h after treatment in the laboratory and at 10th day post-treatm...

JAVERIA NEHAL1, ARIF MUHAMMAD KHAN1, AZHAR ABBAS KHAN*2, MUHAMMAD MUBIN3, HAFIZ MUHAMMAD TAHIR4 & JAVED IQBAL5

...stify;">The conventional method for the detection of Xanthomonas axonopodis has been based on biochemical tests. A rapid and sensitive method for identification and detection of Xanthomonas axonopodis is required for management of citrus canker. PCR-based diagnostic test is appropriate for monitoring pathogen in a very short time compared to laborious, non-specific and expensive protocols. ELISA, Nested PCR has been used for...
ASMA ABDUL LATIF*1, SAMREEN MUSHTAQ1, SABIHA FAZAL1, MUHAMMAD MANSHA2, & ATIF YAQUB3
...unosorbent assay (ELISA) method. The overall seroprevalence of T. gondii in pregnant women was found 22% being IgG positive. The infection rate of T. gondii was observed higher (29%) in older age group (29-39 years) as compared to (18%) in younger age group (18-28 years). More seropositive women were found in 3rd trimester (26%) as compared to 1st (22%) and 2nd (15%) trimester of pregnancy. The highest seroprevalence of T. gondii was found in women having weig...

MUHAMMAD ASHFAQ*1, QURAT-UL-AIN ASGHAR1, RAHILA HAFEEZ1, AMNA ALI1, MUHAMMAD SALEEM HAIDER1, MUHAMMAD ALI1, MUHAMMAD BILAL CHATTHA1 ABDUL RASHEED1 & MUHAMMAD SAJJAD2

... plate and blotter paper method. Highest percentage of myco-flora (17%) was found to be associated with seeds of Basmati Pak that was considerably more than myco-flora associated with all other varieties of rice. The highest percentage of bacterial isolates (16%) was recorded with Basmati Shaheen variety. Total of six fungal and three bacterial species namely Fusarium sp., Alternaria alternata, Pyricularia sp., Dreschslera sp., Penicillium sp., Curvularia sp.,...
SAEED AHMED*1, ZIA-UR-RAHMAN1, SALMA KHALID2, TARIQ KHAN1, ARSHAD IQBAL3
& ZAMARUD SHAH4
...tamination by pour plate method, membrane filtration technique and MacConkey agar for the isolation of Enterobacteriaceae. Different identification tests have been carried out by using various selective and differential media (MacConkey agar, Eosine & Methylene Blue agar) for isolation and identification of microorganisms.Water samples showed the presence of faecal contamination; especially coliform bacteria which is a risk for the health of local communit...

AISHA, MUHAMMAD ASAM RAZA* AND UMME FARWA

... prepared via three-step method using cheaper reagents. The compounds were characterized by employing different spectroscopic techniques and subjected to biological evaluation. In vitro model was used for enzyme inhibition activity through spectrophotometer and suggested that compounds may be helpful in the medicinal field. Compound 2 exhibits more inhibition against BChE (67.7%) while compound 1 showed maximum inhibition of α-glucosidase (65.1%). Theore...
 
 SADAF SARFRAZ1*, SAFDAR AMEER1, REENA AMBREEN1, SHOMAILA SIKANDAR 2, SHAISTA NAWAZ3 AND YASIR SALEEM
...ted using well diffusion method against various bacterial and fungal strains. As shown by the findings of the present study, Cinnamon oil is found a potent antibacterial agent against all microbes used. Additionally, it was found that cinnamon samples collected from Sri Lanka has the highest oil content (3.1%), followed by China (2.8 %) and India (1.9 %), where all tests were standardised using fixed oil levels. In t...
 
 ANSA KHALID, UZMA HAMEED* AND IKRAM-UL-HAQ 
...by using the shake flask method. The isolate IIB-C9 was selected for the parametric optimization as it gave the maximum activity. Among the 10 different fermentation media screened for enzyme production, maximum dextransucrase activity (9.96 U/ml) was obtained by bacterial strain IIB-C9 in fermentation medium M6 comprising (yeast extract 15 g/L, sucrose 40 g/L, and Na
 
 ANSA KHALID, UZMA HAMEED* AND IKRAM-UL-HAQ 
...by using the shake flask method. The isolate IIB-C9 was selected for the parametric optimization as it gave the maximum activity. Among the 10 different fermentation media screened for enzyme production, maximum dextransucrase activity (9.96 U/ml) was obtained by bacterial strain IIB-C9 in fermentation medium M6 comprising (yeast extract 15 g/L, sucrose 40 g/L, and Na
 
 MUHAMMAD WAHEED MUSHTAQ1*, NAUMAN JAMIL KHAN1, NAVEED ASLAM DOGAR1, AISHA HAMEED2, ABDUL HAMEED2, MALIK ADIL2, HAROON IFTHIKHAR3 & SAIRA ISHAQ
...rate, sensitive and fast method to determine concentration of vitamin C (ascorbic acid) from leafy vegetables. So, concentration of Vit. C was measured spectrophotometrically by making a red color complex between 1, 10 Phenanthroline and Vit. C in the presence of ferric chloride (FeCl3). This colored product has maxim...
 
 USMAN AHMAD*1, AMTUL JAMIL SAMI1, MADEEHA KARAMAT1, MADEEHA KHALID1, FAIZAN NAEEM2 & MUHAMMAD JAMIL YOUSAF
...ltrasonic and electrical methods. Drug delivery through the largest organ (skin) by dermal patches of psyllium based hydrogels was observed. In this article we have fabricated different psyllium based dermal patches of hydrogels for insulin delivery. Psyllium, a natural polysaccharide, is a dietary fiber and widely utilized in gel forming. To study the structural aspects of these various polymeric webs were categorized with FTIR, SEM and release related experi...

Ahmed Abdel-Rady3*, Mohamed Karmi2, Menna_allah Youssef1, Aml M. Abdel-Ra’ouf1, Bahaaa Madkour1 

...ed with the conventional method for diagnosis of bovine tropical Theileriosis. PCR proved a highly sensitive and accurate method for diagnosis of bovine tropical Theileriosis especially in detection of blood and lymph smears negative cases.
 

...

Irene S. Gamil1* and Dalia Fouad1,2

...rnorate, Egypt. Standard methods of collection of the snakes and examination of the cestode tapeworms for taxonomic studies were used. Ophiotaenia tessellata sp. n. was identified and being separable from Ophiotaenia species found in African snakes as well as those from colubrid snakes based on many morphological characteristics. Analysis of a dataset based on 473 bp of its 18S rRNA gene regions was carried out to determine the phylogenetic position of the new...
Nazish Huma Khan1*, Muhammad Sufyan Khan1, Tooba Saeed2, Mohammad Ilyas3 and Hazrat Husaain4
...medical and conventional methods need to be adopted depending on the severity of viral attack. Moreover, vaccination is also referred to improve the immunity against this viral attack.
...

Theresia Nur Indah Koni*, Tri Anggarini Yuniwati Foenay, Stormy Vertygo

...nt of 14-16%. One of the methods to reduce crude fiber is through anaerobic fermentation, which requires carbon from soluble carbohydrates such as palmyra sap and nitrogen from Non-Protein Nitrogen such as urea. This experiment aimed to evaluate the fermentation characteristics and nutrient content of rice bran fermented anaerobically for six days. This experiment used a Completely Randomized Design with four treatments and four replications. The treatments in...

Imran Khan1*, Shahid Amin2, Safiullah3, Shah Zeb Khan3, Abidullah3, Amjad Ali3, Saghir Imdad Hassan3 and Israr Ud Din3

...smears techniques as per method described by (Adam et al. 1971). A higher infection rate was found in females (6.67%) as compared to males (4.67%). Highest (9.00%) infection was noted in camels above 4 years of age group followed by (5.00%) in camel under 2 years age group while lowest (3.00%) in 3-4 years age group camels. It was concluded that 5.67% (17/300) samples were found positive for Trypanosoma evansi infection. Infection rate was found higher in fema...

I Nyoman Sulabda1, Anak Agung Gde Oka Dharmayudha2, I Wayan Nico Fajar Gunawan3, Dwi Fortuna Hashilonda4, Luh Gde Setyawati5, I Ketut Puja6*

... artificial insemination method. Eight bitches were used in this experiment. The dog was in good health. The time of artificial insemination was performed 11 days after the onset of proestrus. Bitches are inseminated intravaginally. Pregnancy was confirmed by ultrasound 25 days post-AI. To our knowledge, this is the first successful case of AI in a Kintamani dog. From these results, it can be concluded that freezing the semen of Kintamani dogs could fertilize ...

Imran Muhammad Siddique1, Naseem Sharif1*, Abdul Ghaffar2, Mudassar Naseer1, Ammara Noreen3, Naseem Akhtar4 and Safeer ud Din5

...cters through vegetative method of propagation thereby avoiding inevitable heterozygosity and polyembyonic nature of citrus fruits.

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Faradji Khalil1*, Slimani Noureddine2 and Senoussi Abdelhakim3

...e with cool winters. The methodological approach followed to carry out our study requires the use of appropriate observation or survey methods and the use of analysis means adapted to the situations encountered. In this context, the means used to carry out this work are based on documentary research, surveys and observations. Based on field investigations, three main categories of livestock breeders were distinguished the fa...

Ijaz Haider1,2*, Muhammad Riaz2, Sikandar Ali2, Qurban Ali1, Ali Noman3, Dilbar Hussain1, Imran Nadeem1, Muhammad Faheem Akhtar1, Aqsa Abbas1, Asad Aslam1, Hafiz Saad Bin Mustafa2, Ejaz Ul Hassan2, Muhammad Zubair2, Muhammad Saleem1 and Muhammad Kamil Malik1

...cluding chemical control methods. Due to certain environmental issues and insecticide resistance problems, adoption of alternate methods of insect control is imperative. In this experiment we used some insecticides alone and in combination with Salicylic acid against B. tabaci. Flonicamid 50% DF at the rate of 80 g/ac and Diafenthiuran 50% SC at the rate of 200 mL/ac were used against Whitefly. Salicylic acid (SA) by Sigma-A...

Shaimaa M. Ahmed1, Gehan M.A. Kassem1*, Fernando Pérez-Rodríguez2, Heba H.S. Abdel-Naeem1 

...urbidity and plate count methods as well as monitoring and modelling its potential growth in chicken breast and thigh meats at room temperature (25 °C) and refrigeration temperature (4 °C). The calibration curve showed a linear relationship between the turbidity measurement and bacterial cell count in TSB. Moreover, the coefficient of determination index (R2) was 0.8789 which indicates that the linear function was acceptable for reflecting the relation...

Mostafa Mohamed Attia Hammam, Moawad Mohamed Mohamed Mohamed* and Mahfouz Mohamed Mostafa Abd-Elgawad

...esents effective control methods as Egypt is the world’s largest importer of faba bean. Using gain thresholds, the study compares costs of a biological versus a chemical nematicide for M. arenaria control on faba bean. Meloidogyne arenaria was not detected at pre-planting soil samples of two consecutive field seasons. However, the potential of field weeds as hosts/reservoirs for M. arenaria to transfer from weeds and build-up on ‘Giza 843’ fa...

Ahmad Jupri1*, Yuliana Vofi2, Faturrahman2, Immy Suci Rohyani1, Ernawati1, Bulkaini3, Djoko Kisworo3, Wardatul Jannah4 

...-June 2021. The research method used was purposive sampling with sampling carried out at three points: the spring, the main reservoir, and the residents’ reservoir. The Coliform test was carried out using the Most Probable Number (MPN) method and Escherichia coli (E. coli) identification using Kirby Bauer. The results of this study indicate that the coliform MPN test from the spring sample obtained the highest value ...

E. K. Allam.1; B. A. Othman.1; Hayam S. Abdelkader2; and Noher A. Mahmoud2

... and molecular detection methods provide tools assisting in the understanding of the epidemiology and diversity of nepoviruses as well as to facilitate resistance breeding, cultivar selection, and development of control strategies.

...

Hayam S. Abdelkader1; Gamalat M. Allam1; T. A. Moustafa2; and M. El-Hanunady2

...ng the two isolates. The method described here is rapid. reliable and highly recommended to be used in such virological and pathological studies.

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E. K. Allam1; Kh. A. El-Dougdoug1; M. A. Amer2; A. A. Abou-Zaid2; and S. M. Amin2

...olecular techniques. The method involved the extraction of circular nucleic acids followed by electrophoretic analysis of the extracts on 5% polyacrylamide gel. A reverse transcription-polymerase Chain reaction (RT-PCR) assay was used for the isolation and identification of PSTVd cDNA from infected plant materials using a specific primer to PSTVd. A major cDNA product of approximately 360 bp was successfully hybridized with digoxigenin labeled PSTVd cDNA probe...

Sahar. A. Shoman1 and B. A. Othman2

...nk) provided a sensitive method for the detection or Tobacco mosaic virus- Egyptian strain (TMV-E). Total RNA extract of infected plant and RNA of purified TMV particles were subjected in this method with four degenerate and undegenerate primers. The primer pairs generated two specific PCR fragments of 3428 base pair (bp) and 3836 bp of the whole TMV genome. The intensity of these RT-PCR amplified products was estimated, and...

M.A.S. El-Kady1, Om-Hashem M. El-Banna 2, E.A Salama2, Salwa N. Zein1

...n of BSMV by serological methods i.e., Enzyme-linked immunosorbent assay (ELISA), Dot-blot and tissues-blot immunobinding assays (DBIA and TBIA). The presence of the virus was confirmed in mature seed parts and non-seed parts of the different five barley cultivars tested.

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Waheed Khan1, Muhammad Ajmal Khan2, Bakhtawar Khan1*, Aftab Amin3, Muhsin Ali4, Awais Farid, Ali Hazrat5 and Muhammad Yahya5

...ded to investigate novel methods to improve DM treatment. Thus plant-based management could be a possible antidiabetic strategy. The objective of the most recent study was to assess Verbascum thapsus’s ability to combat hyperlipidemia and diabetes. Mice were given an intraperitoneal injection of alloxan (150 mg kg-1, b.w.) to cause diabetes. There were five groups of mice (n=10): group 1 (normal control) received normal food and water, group 2 (diabetic ...

Muhammad Ismail1, Abu Bakar Muhammad Raza1,2, Zarina Qasim3, Muhammad Zeeshan Majeed1*

...l available pest control methods with minimum implication of hazardous synthetic insecticides. In this study, five treatments including field sanitation (T1), pheromone-based attract and kill strategy (T2), protein-based bait method (T3), combination of all three methods (T4) and control (T5) were evaluated against B. dorsalis infestation in citrus during 2015 and 2016. Data of percent inf...

Jaswandi, Ananda*, Rusfidra, Kusnadidi Subekti, Harif Gusdinal, Raziah Sri Wahyuni, Fahmi Ariansyah Caniago 

...pment using the candling method. Observation of fresh semen of the KBC showed that macroscopically the semen of the KBC was white, thick, and had a volume of 415 ± 218.08 µl. While the results of the microscopic evaluation showed that the semen quality of the KBC was a positive mean mass movement of 2 (++), spermatozoa motility was 79.71 ± 2.91 %, spermatozoa concentration was 2432.34 ± 668.08×106, spermatozoa viability was 93....

Behari Lal Meghwar1*, Attaullah Khan1, Imran Ali Lakhiar1, Asif Ali Mirani2, Muhammad Siddique Daper1 and Muhammad Waseem Kalroo

... sun (T3) or traditional method; this practice has many drawbacks and leads to poor quality. A study was planned to find an alternative chili drying method for the study area. To achieve the desired goal, we considered two different drying methods, solar tunnel (T1) and solar cum gas (T2), and compared the rate of reducing the moisture over time with T3 method

Pengliang Xie1, Lufang Zheng2*, Mingxia Dong3, Huaiqiang Zhang1 and Fang Chen1

...nd develop new treatment methods.

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Muhammad Arshad Iqbal*, Aamir Ghafoor, Irfan Ahmad, Muhammad Ijaz 

...acteria through dilution method. 4 birds/ group were slaughtered at d35 and d42, liver, spleen, intestine, and ceca were collected to observe organ colonization. Use of Bacillus amyloliquefaciens as feed additive decreased the shedding pattern of S. Enteritidis and Cl. Perfringens and may pave way for safe poultry rearing and addressing public health concerns associated with AGP.
 

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Maria Endo Mahata1*, Oriyanti Br Situngkir1, Yan Heryandi2, Takayuki Ohnuma3, Yose Rizal1 

...udy used an experimental method with a completely randomized design (CRD) of a 3x3 factorial pattern which was repeated three times. Factor A (type of pellet binder): A1 (brown seaweed), A2 (taro tuber), and A3 (tapioca flour), then factor B (dosage of pellet binder): B1 (1.5; 3.3; and 4.5%). Crude fiber digestibility, nitrogen retention, and energy metabolism were measured. The results showed no interaction (p>0.05) between the type of pellet binder and do...

Shaimaa A.Tawfik2,3, EL S.T. Awad1*, Hoda O.Abu Bakr1, Amira M.Gamal-Eldeen4, Esmat Ashour2, Ismail M.Ahmed1  

Luqman1*, Zahid Hussain2, Tamana Bakht3, Fazli Wahab1, Miftah-ud-Din1, Haroon Khan2, Imran Shinwari1, Ata Ullah1, Liaqat Khan1 and Sara Hidayat

Kétomon Pierre Challaton1*, Coovi Guénolé Akouedegni1, Kadoéito Cyrille Boko2, Goué Géorcelin Alowanou1,3, Pascal Venant Houndonougbo4, Aboudou Habirou Kifouly1, Mawulé Sylvie Hounzangbé-Adoté1  

...e study and the Baermann method for the qualitative research. The results showed an overall prevalence of gastrointestinal parasites of 96.82 %. Furthermore, goats were mainly infected with coccidia (92.24 %); strongyles (83.91 %), of which the critical genera were Haemonchus sp., Trichostrongylus sp., and Oesophagostomum spp. ; Strongyloides spp. (73.25 %) followed by Moniezia spp. (21.8 %). Other gastrointestinal parasites retrieved were Trichuris sp. (0.94%...

Yue-Xia Ding1, Qun Wu2, Zhao Namula1 and Yi Ma1,*

...t by membrane filtration method. The correlation between antimicrobial resistance and virulence traits was analyzed by LD50 and related genes. Sensitive Streptococcus isolates to erythromycin and S. pneumoniae ATCC49619 were induced to resistance in vitro, MIC value was from ≤ 0.5 μg/mL up to ≥ 64 μg/mL, and ermB or mefA resistant gene were carried. Transfer rate of resistance was 100% by plasmid conjugant, conjugants had obtained the resistance ph...

Atena Azarniush1, Majid Moghbeli2*, Farshid Kafilzadeh1, Mohammad Kargar1 and Hooshang Jamali1

...therapy is a therapeutic method in use to relieve this condition. It may lead to infections due to the injection, as well as platelet resistance. Therefore, an attempt was made to find an alternative treatment, and the recombinant Interleukin 11 (IL-11). In this study, IL-11 gene owning two restriction enzyme sites of BamHI and XbaI with a final size of 609 bp was synthesized by GenRay (China) and delivered to pGS vector. The vector was transferred to DH5&alph...
Widya Pintaka Bayu Putra1, Herman W Raadsma2, Indriawati1, Slamet Diah Volkandari1 and Endang Tri Margawati1*
...ysis of variance (ANOVA) method. A total of 650 records of LS were used in this study, representing in the progeny of fourteen (14) rams collected from year 1999 to 2004 at Cimanglid Research Park, Bogor, Indonesia. This study showed that the average of LS in sheep samples was 1.34±0.51 with h2 value of 0.04±0.06 (low). However, six Merino rams (43%) had positive of EBV value. Moreover, the data records of LS from each observed ram were sufficien...

Yong Bin Zhao1, Hui Song1, Guo Gong Yi1*, Xi Lian Hao1, Bai Wang2, Zhi Wen Chen2

...ing is the only feasible method for isotopic research. However, whether it can be used for research on individuals remains to be clarified. In this study, the values of δ13C and δ15N of the rachises and barbules of 27 feathers from 3 pigeons were measured, and cluster analysis of these samples was carried out by using the system clustering method. The results showed a significant difference between the isotope va...

Abid Hussain1*, Hassnain Shah1, Muhammad Nadeem Iqbal2 and Tariq Sultan3

... wheat crop by zero-till method. Keeping this in view, economic viabilities of these technologies have been determined through marginal analysis. Unfortunately, Pak-seeder technology could not be demonstrated effectively due to few institutional and technical limitations. Thus, technical issues in its manufacturing as well as its use are highlighted. The study is based on the outcomes of six demonstration sites of micronutrients’ use for citrus and thirt...

Nitya Salsabila, Djalal Rosyidi, Agus Susilo* 

...iander and tamarind. The method used was a laboratory experimental method using a completely randomized design (CRD) with 6 treatments and 4 repetitions, namely P0: without the addition of liquid smoke (control), P1: liquid smoke without sonication, P2: sonication liquid smoke for 5 minutes, P3: sonication liquid smoke for 10 minutes, P4: sonication liquid smoke for 15 minutes, and P5: sonication liquid smoke for 20 minutes....

Natalia Shchur1,2*, Olha Chechet1, Tetiana Mazur2, Oleksandr Martyniuk2, Olga Gorbatiuk1, Halyna Buchkovska1, Iryna Musiets1, Diana Ordynska1, Olena Finkova3, Larisa Moskalenko3, Tetiana Ponomaryova-Gerasimyuk3, Maksym Lusta3, Vitalii Nedosekov2 

...zed by a microbiological method for the detection of Campylobacter according to the scheme of accumulation, isolation, isolation and identification according to DSTU ISO 10272-1:2007. As a result, 33 isolates of Campylobacter spp. were isolated, which is 1.6% of the total number of the researched samples. The most common phenotypes of antimicrobial resistance of the selected isolates were: Cip / Tet / Ery - 14 isolates from poultry, which accounted for 42...

Khitam J. Yahya*, Mohammed T. S. Al-Zubaidi 

...ium spp. using molecular methods (PCR), followed by DNA sequencing. The overall infection rate was 37% (37/100) of the samples were Cryptosporidium-positive by using PCR. Three of five quails whose 18S gene was amplified by nested PCR and sequenced were found to belong to C. meleagridis in the sequence of this gene deposit on the website of the National Center for Biotechnology Information (NCBI) under accession number (HQ917077.1), while the others were found...

Bambang Hartoyo1*, Tri Rachmanto Prihambodo1,2, Wahyuningsih3, Sri Rahayu1, Fransisca Maria Suhartati1, Muhamad Bata1, Efka Aris Rimbawanto1

...ed using the mixed model method with different study as random effect and level of lactobacillus as fixed effect using SAS program. Lactobacillus as potential feed additive had significant influence (p<0.05) to improve performance such as average daily gain (50.28 g), average feed intake (93.57 g) and feed conversion ratio (1.91) of broilers in ameliorate condition of digestive tract by decreasing the amount of Escherichia coli. Due to pathogen bacteria dec...

Nawfal Hammadi Jasim1, Yasmeen Jassim Mohammed2 , Jala Amir Salman Alahmed1, Majdy Faisal Majeed3* 

...his study. Materials and methods: receiving an oral dose (2 daily MPH doses), represented by Group 1 (5 mg/kg/day in a 5% glucose solution), Group 2 received daily dosages of methylphenidate in escalating amounts (5, 8, 12, and 16 mg/kg/day in a 5% of sucrose solution), as well as Group 3 as control animals ( 5% glucose solution). Group 1 resembled the therapeutic doses administered to humans, while Group 2 simulated the wrong use of some of the human addicts...

Budi Guntoro1*, Agung Triatmojo1, Bambang Ariyadi2, Nguyen Hoang Qui1 

...ulti-stage random sample method and quota sampling. Descriptive statistics were used to analyse primary data from the survey. Farmers were chosen by the criteria of who are staying and raising beef cattle in Yogyakarta. The results showed that farmers had a high experience and more than 50 years old, they worked as a farmer at the farm with low income from cattle production (less than 100$). Farmers had more than 8 years of education and raised their cattle un...

Winda Sartika1*, Budi Hartono2, Hari Dwi Utami2, Lilik Eka Radiati2 

...esearch using the survey method, snowball sampling in the rendang industry with raw beef, and purposive and accidental sampling on the supply chain institutions involved in rendang production. The research respondents comprised 61 respondents from the rendang industry, including suppliers of raw materials, distributors, and consumers of rendang with qualitative and quantitative descriptive data analysis. The results of the study show that the resilience of the...

Jawad Ali1,*, Samiya Rehman1, Aqsa Aslam1, Fouzia Tanvir2, Rida Liaqat1, Muhammad Ahmad1, Aamir Riaz1, Muhammad Shafique3, Hassan Ali1 and Amjad Ali1

...PH and Phosphomolybdenum methods. Results shows leaves extract from Mianwali has the highest total antioxidant capacity while stems collected form the Sargodha has highest free radicals scavenging ability with values of 47.60± 3.00 Vit C equiv mg/g DW and 50.00± 3.00 IC50 value. 

...

Tasew Getu1*, Wassu Mohammed2, Awol Seid3, Tesfamariam Mekete4 and Bekele Kassa

...the effect of irrigation methods, cultivars, vermicompost (VC) and their interactions for management of the MI and RS disease complex under field conditions. The experiment was laid out in a split-split plot design with three replications. The findings indicated that the treatments and their interactions significantly (P ≤ 0.05) affected the development of MI, RS, and impacted potato yield. The cultivar Gudenie had the lowest mean value (2.2) of MI reproduc...

Fakhra Nazir1*, Sahar Fazal1 and Fakhar-i-Abbas2

...ed out by Sanger’s method and BLAST analysis identified these samples as five species (Urocissa flavirostris, Dendrocitta vagabunda, Corvus splendens, Corvus corax and Corvus macrorhynchos) from three genera of family Corvidae. The nucleotide sequences were submitted to the Barcode of Life Data System (BOLD) and the eligible sequences were assigned the Barcode Index Numbers (BINs). The COI gene sequence data of 8 species of family Corvidae were retrieved...

Meltem Kızıl1*, Ali Rişvanlı2, Murat Abay3, Tarık Şafak4, Mehmet Akif Kılınç5, Öznur Yılmaz6, Burak Fatih Yüksel1 and İbrahim Şeker1

...were determined by ELISA method. Feeding with colostrum led to a decrease in the irisin levels in the dystocia and cesarean section groups and in the IGF-1 levels in the vaginal delivery group, but it did not lead to any other significant changes. The irisin levels of the calves in the vaginal delivery group were compared to those in the other groups, and their levels were found to decrease on the 15th day, and IGF-1 levels were higher on the 15th day. As a re...

Hamdi Mayulu1*, Endang Sawitri2

...ve. The Folin-Ciocalteau method was used to test for tannins and total phenolics, and the aluminum chloride (AlCl3) method was used to assess flavonoid content. In addition, antioxidant capacity was determined with Radical Scavenging Activity 2,2-diphenyl-1-picrylhydrazyl (DPPH) and expressed with IC50 value. The data obtained were analyzed using the single factor Analysis of Variance (ANOVA) with the IBM Statistical Package...

Khalil1*, Dwi Ananta1, Andri Bachtiar2, Hermon3

...a practical bulk storage method to maintain the palatable component, moisture, nutrient content, and nutritional value of rice straw supplemented with 1% calcite-based mineral mixture, 0.6% molasses, and 0.05% urea. The supplemented straws were stored for 60 days in three bulk treatments: loose stacking, open-tied rolling, and airtight wrapping. The changes in physical appearances, botanical fraction, proximate, and fiber fraction content were determined. The ...

Ahmad Noor and Malik Muhammad Shafi*

...infall was only recharge method. Boreholes pumping technique was used mostly for irrigation. The Logit model investigated the impact of climate change was responsible for 73 percent variation in groundwater level. These climate factors included precipitation (Rainfall), temperature, humidity and delayed seasons. Rain patterns such as decrease in rainfalls with low frequencies showed positively significant impact on the groundwater level as climate factor and d...

Fadzlin Afiqah A. Samad2, Amirul Faiz Mohd Azmi1, Muhamad Affan Ab Azid1, Hafizin Mu’izz Zalazilah1, Syakirah Zulkifli1, Izreen Edriana Mohd Jasmi1, Muhammad Baqir Irfani Rahimin Affandi1, Mohd Zamri Saad1, Md Zuki Abu Bakar1, Agung Irawan3,7, Adib Norma Respati4, Anuraga Jayanegara5,7, Sadarman Sadarman6,7, Hasliza Abu Hassim1,2,7*. 

... to a linear mixed model methodology with explanatory variables declared as fixed effects and individual study as random effects. The results showed a negative curvilinear pattern of milk yield across buffaloes’ breeds in response to the increasing FC ratio (P<0.05; R2 = 0.828) and strong linear increased in response to the increasing DMI (P<0.01; R2 = 0.841). The interaction effect was found between breed of buffaloes and NDF content of the diets ...

Salvator Minani1,2*, Eric Nsengiyumva1, Anatole Bigirimana1,2, Arnaud Cubahiro1,2, Dieudonné Ntakirutimana1,2 and Vénuste Bizoza1,2

...rundi. Effective control methods of liver flukes for intermediate and definitive hosts should be implemented to control fascioliasis in Burundi.

...

Chairdin Dwi Nugraha1, Rafika Febriani Putri1, Ari Ardiantoro2, Wike Andre Septian1, Ahmad Furqon3, Kuswati Kuswati1, Nashi Widodo4, Suyadi Suyadi1* 

...rch used an experimental method to examine the effect of genistein levels in the diluent on the characteristics of spermatozoa during pre-freezing at 4–5 0C and post-thawing (PT). The data obtained in the form of quantitative data were analyzed descriptively and analysis of variance patterns in One-Way ANOVA. 0 µM, 10 µM, 30 µM and 50 µM genistein treatment in the basic diluent tris aminomethane–egg yolk. Progressive motilit...

Antonius Agung Wiono1, Maria Elizabeth Nana Wijayanti1, Dhika Yonika Primacitra1, Trioso Purnawarman2, Andriyanto3, Aulia Andi Mustika3, Amaq Fadholly3* 

Luigi Liotta1, Vincenzo Lopreiato1, Fariborz Asroosh2 and Alireza Seidavi2*

...ccording to the standard methods. Macro and micro elements of milk were analyzed using inductively coupled plasma mass spectrometry. Data were subjected to the two-way ANOVA analysis to determine the significant differences between species/breeds. The acidity, density and fat in Talesh sheep’s milk was higher than milk from other species/breeds (P<0.01). Also, the amount of protein in Talesh sheep’s milk was significantly higher (P<0.05) than...
Muhammad Rizwan1, Hamid Akbar1*, Aftab Ahmad Anjum1, Muhammad Arif Khan1, Aneela Zameer Durrani2, Muhammad Abid Hayat3, Anjum Masood4, Muhammad Talha Sajjad1 and Nadeem Raza2
...y authenticated standard methods. We observed that serum levels of MDA and BHBA were higher at day 0 to day 14 (P<0.01), while the level of nonesterified fatty acid (NEFA) was higher at day 0 to day 7 (P<0.01) which dropped down to normal at day 21 (P>0.05) post-surgery in Group-B than Group-A. Moreover, CAT level in serum decreased (P<0.01) at day 0 to day 7, then it gradually increased to normal on day 14 to day 21 (P>0.05) post-surgery in Gro...

Trinil Susilawati1*, Aulia Puspita Anugra Yekti1, Amir Firdaus2, Dinda Ayu Damayanti2, Rizki Prafitri1, Nanang Febrianto1, Kuswati1, Achadiah Rachmawati1, Sri Wahjuningsih1, Nurul Isnaini1 

...eeds to be improved. The method of Double-dose AI can highly improve the success of AI. The materials used were 160 cows divided into 80 cows inseminated by a single dose and 80 cows inseminated with a double dose. There were two treatments in this research; T1 was AI single dose at the 8th hour after estrus onset, while T2 AI double dose at the 2nd and 8th hour after estrus onset. The Non-Return Rate (NRR 1) was observed on the next cycle after AI. If the cow...

Mehwish Malik1*, Zanib Sadia2, Muhammad Sajid3, Hammidullah1, Yasir Amin1, Zubair Ali1 and Sohail Ahmad1

... microbe’s culture methods and biochemical tests. The E. coli isolated is further tested for ESBL E. coli. The detection of ESBL E. coli is done through DDST and CDT. It was noted that out of 210 samples 08 samples were found negative (no growth of any bacteria found) and 20 samples were shown growth other than E. coli suggesting a total prevalence of E. coli as 86.6 %. The prevalence of ESBLs in cecum of broilers in the present study is 53% of the total...

Isatay Tusupovich Jakupov, Gulzhan Tursunovna Yeszhanova, Gulnur Kurbanalievna Mamytbekova* 

...ere examined by clinical methods after calving, of which 45 cows showed signs of acute endometritis. Subsequently, the bacterial background of uterine mucus in sick cows was investigated and St. pyogenes, E. faecalis, and E. coli were isolated. For the complex treatment of acute postpartum endometritis, the cows of the experimental groups were treated with the phytopreparation C. leucocladum. For comparison, the drug «Biometrosanit» was used in the...

Altaf Ur Rahman1, Muhammad Ajmal Khan2*, Ali Hazrat3, Awais Farid4, Muhammad Yahya3* and Inam Ullah5

...gated via well diffusion method by using a concentration of 100 µg/ml. Furthermore, the antioxidant potential of extract was estimated at various concentration (50, 100, 150, 200, 250 µg/ml) through 2, 2-Diphenyl -1- picryl-hydrazyl (DPPH) assay (150 µg/ml). Results showed that the methanolic extract of C. jacquemontii is a rich source of many phytochemically active compounds such as alkaloids content (3.60%), flavonoids (3.40%) and terpenoid...

Abdullah*, Imtiaz Khan, Muhammad Ishfaq Khan, Muhammad Ibrahim and Muhammad Fawad

...rict Karak. The quadrate method was used to determine the relative weed density. The data were recorded on density (%), relative density (%), frequency (%), relative frequency (%) and importance value of weed species. The present findings revealed that Asphodelus tenuifolius Cav., Carthamus oxycantha M. Bieb., Medicago denticulata Willd., Anagallis arvensis, Lathyrus aphaca L. Euphorbia helioscopia L., Convolvulus arvensis L., Cyprus rotundus, Vicia sativa L. ...

Komal Javed1, Sohaib Muhammad1*, Zarghaam Khan1, Sehar Fatima1, Hassan Nawaz1, Mahrukh1, Tahira Khalid1 and Shariat Ullah2

...th the help of a quadrat method (1m2). For each crop, sixty quadrats were taken from each village i.e., twenty quadrats from each selected site. The data was gathered after every fifteen days during the course of crop. For the determination of Importance Value Index (IVI) percentage frequency, density and percentage cover were determined. A total of 19 families were present in all three crops. Fumaria indica of Family Papaveraceae had the highest IVI value of ...

Chitra Kumalasari1,2, Lovita Adriani2*, Indrawati Yudha Asmara3, Nazri Nayan4 

...s supplementation is one method of increasing productivity in old age laying hens. This review explores the potential of probiotics that can increase egg production in laying hen late-phase, which discusses the reproductive system, including hormones and the reproductive tract, productivity, digestive system, and health of laying hens at an advanced age. This article attempts to provide a complete summary of the potential benefits of probiotics in prolonging t...

Yakun Ge

...tion was detected by MTT method. Combined treatment promoted the production of ROS. The cell viability of the combined treatment group was lower than that of the control group at 24h, 48hs and 72h (P<0.05). Compared with the control group, the expression level of E-cadherin mRNA in the combined treatment group increased (P<0.05), and the expression levels of N-cadherin and vimentin decreased (P<0.05). Compared with the control group, the apoptosis rat...

Alisher Safarov1*, Nasreen Nasreen4, Firuza Akramova5, Shukhrat Djabbarov1, Adolat Mirzaeva5, Javokhir Esonboev5, Djalaliddin Azimov5, Mourad Ben Said2,3 

Luthfiralda Sjahfirdi1*, Zulfi Atsiil Suandhy1, Tatang Mitra Setia2 

... all-occurrence sampling methods. Nest-building skills were assessed using the Likert scale. Moni (male orangutan) was always observed to nest using used nests. Meanwhile, Terra (female orangutan) was frequently observed building new nests, although she was caught sleeping on the forest floor several times. During observations, it was found that they prefer nest trees with a circumference of more than 1 m with a reasonably dense canopy refers preferred trees t...
Maria Azam1, Tahir Mahmood Qureshi1, Saddam Hussain2, Amjad Islam Aqib3*, Shanza Rauf Khan4, Kashif Akram1, Misbah Ijaz5, Maheen Murtaza6, Afshan Muneer6 and Sammina Mahmood7
... and broth microdilution method. To analyze the data, both probability and non-probability statistical tools were applied using SPSS version 22 of statistical software at 5% probability. The current study showed 24.56% of S. aureus positive from commercial dairy while the resistance of these isolates against gentamicin, enrofloxacin, levofloxacin, and vancomycin was found to be 50, 40, 30, 30%, respectively. On the other hand, a disc diffusion assay was conclu...

Rashid Saraz1, Saiqa Amur1, Zia-ul-Hassan1*, Naheed Akhter Talpur1, Inayatullah Rajpar1, Muhammad Sohail Memon2, Muhammad Nawaz Kandhro3, Khalid Hussain Talpur1 and Nizamuddin Depar4

...re (Bouyoucos Hydrometer method), electrical conductivity and pH (1:2 soil-water extract), organic matter (Walkley-Black method), and ABDTPA (Ammonium bicarbonate diethylene triamine penta acetic acid) extractable phosphorus (P) and potassium (K) were determined using standard protocols, with no further alterations. Soil variability mapping was done using ArcGIS ver. 10.7. through IDW interpolation. The results reveled that ...

Afifatul H.A. Adilah1, Junun Sartohadi2* and Suci Handayani1

...ted using a field survey method equipped with soil characteristic measurements in the laboratory. The data collection process began with a study of the landscape of the research area which was divided into three zones, namely residual, erosion, and deposition. In each zone, coconut and Mahogany plants were selected with the ideal morphology for measurement of interception, throughfall, and stemflow during rain events. Soil sampling under the plant canopy was u...

Nadia Jabeen1, Abdul Mubeen Lodhi1*, Rehana Naz Syed1, Muhammad Ali Khanzada2 and Alia Gul3

...n the maximum similarity method using Mega 6.0. The spore was smaller as compared to Marasmiellus scandens (6.2-8.7 µm). The newly described species i.e., Marasmiellus agrianum N. Jabeen and M. Lodhi differs from other species based on the molecular study as well with few morphological characteristics; size, color, shape, and texture of pileus, and stipe, and the molecular characterization reveals that the species was placed at a new place in the phyloge...

Asim Zubair1*, Anam Farid Chishtti2, Zohaib Atta Mehdie3, Aamna Arshad3, Ghulam Akbar Malik4 and Iffat Batool5

...tified using statistical methods. The findings show that women dominated practically all farming operations in the chosen area.

...

Amthal Ahmed Fouad1, Basem Mohamed Ahmed2, Momtaz Abdelhady Shahein1, Hussein Aly Hussein2* 

Jaisy Aghniarahim Putritamara1*, MB Hariyono1, Budi Hartono1, Hery Toiba2, Hamidah Nayati Utami3, Moh. Shadiqur Rahman2, Tina Sri Purwanti1

...lected using a purposive method. The first inclusion criterion is running a micro, small, or medium enterprise (MSME) in the beekeeping industry (with business sizes determined based on the number of workers, assets, and turnover as stipulated in the Law of the Republic of Indonesia No. 20 of 2008 concerning MSMEs. The second criterion is having been in business for at least ten years. The findings show that IC mediates CKM in achieving CA. There are also posi...

Deny Juniwati1, Hadri Latif2*, Trioso Purnawarman2, Zhang Shuqi3

 

...sis (RO) water, but this method does not significantly reduce nitrite levels. Therefore, an alternative method is needed. This study aimed to analyze the effect of washing using RO water combined with ultrasonic waves to reduce nitrite level of EBN. The result of this study illustrated the effectiveness of reducing nitrite levels in EBN from two different methods. Nitrite levels in the sam...

Satya Fahriani1, Hadri Latif2*, Herwin Pisestyani2, Zhang Shuqi3, Suyanto Chen3

...terilization with retort method. Indonesia’s government does not have data on the effect of retorts on the safety and quality of EBN. This study aimed to examine the nitrite level and rehydration capacity of EBN after undergoing a retort process. The samples were 24 raw uncleaned white EBN from 4 swiftlet farmhouses (SFHs) collected using purposive sampling. Two SFH from Sanggau District, West Kalimantan Province, Indonesia. Two SFH from Pekanbaru City, ...

Saddam Hussein Mahmoud* and Shaimaa Nabhan Yassein

...ed by using conventional methods such as the germ tube test, urease production, Chlamydospore formation, and chromogenic medium. Hemolysis, phospholipase activities, and biofilm formation were among the virulence tested factors. Hemolysis and phospholipase activities were detected in 76.2% and 83.33% of C. albicans isolates, respectively, while biofilm formation was detected in 100% of C. albicans isolates. The diagnosis was confirmed using 25 of the most viru...

Bing Yan1, Meng Meng Zhang2, Xi Chen2, Yongming Li2*, Muhammad Khan3* and Tonghui Ma1*

...t to improve therapeutic methods, the prognosis of GBM still remains very poor. The poor prognosis is associated with cancer stem cells (CSCs) which hold the potential of self-renewal, and differentiation into pathogenomic heterogeneous tumor formation leading to therapeutic resistance. Therefore, targeting CSCs and their associated signaling pathways is crucial for effective treatment of GBM. In this study, we showed that alantolactone (ALT) hold the capacity...
Iram Liaqat1*, Noor Muhammad1, Muhammad Mubin2, Najma Arshad3, Tehreema Iftikhar4, Sumera Sajjad5 and Farzana Rashid5
...lates using cross streak method showed excellent zone of inhibition (ZI 10-27 mm) against tested pathogens (Escherichia coli, Klebsiella pneumoniae, Bacillus licheniformis, Pseudomonas aeruginosa and Bacillus subtilis). Secondary screening using ethyl acetate extracts also showed significant ZI in the range of 06 – 14.0 mm (P ≤ 0.05) against these pathogens thus confirming their bioactive potential. The minimum inhibitory concentration (MIC) and minim...

Sumei Zeng and Yong Zhang*

...onal Assessment (COPRAS) method. Evaluation indicators were selected by theme analysis method based on the opinion of managers working in industries that are using IOT. The research method is applied in terms of purpose and is descriptive in terms of data collection tools. The results showed that Detection of Insemination Time (DIT) and Early Detection of Diseases (EDD) applications are mo...

J. Ma1, F.B. Zhang1,2, J. Li1, Z.Y. Lv1, C. Zhang1, L. Mao1 and Y. Zeng1*

...d the Amundsen graphical method. A total of 26 food categories were identified, among which algae and organic detritus were the dominant food source for S. dabryi. Animal prey contributed relatively low proportions to the food of S. dabryi. Seasonal variations of food composition of S. dabryi were significant (P<0.05). Hierarchical cluster analysis shows that the food composition in autumn was significantly different from other seasons at a similarity level...

Ijaz Ahmad1,5*, Haihong Hao1, Pascal Sanders2, Zafar Iqbal3, Saeed Ahmed4, Farhan Anwar Khan5, Zahir Shah5, Muhammad Ibrahim6 and Lingli Huang1

...24 h at 4oC. The present method has been applied successfully for the pharmacokinetics study in cattle. After intramuscular administration, the mean peak serum Concentrations (Cmax) was found 2.95μg/mL and achieved after (Tmax) 1.50h. The elimination half-life (tl/2(el)) was 2.03±0.06h. The Mean Residence Time (MRT) was 2.58±0.05h and the area under curve from zero time to infinity (AUC0-∞) were 9.67±0.72μg/mL/h. The newly prop...

Feng-Yan Liu1,2,3, Ting Chen1,2,3, Yu-Hui Li1,2,3, Zheng Liu1,2,3, Jing-Jin Zheng1,2,3, You-Bang Li1,2,3* and Zhong-hao Huang1,2,3*

...study, with non-invasive methods, we measured physiological hormones including glucocorticoids (GCs), cortisol, and thyroid hormones (THs, T3 and T4) of rhesus macaques’ (Macaca mulatta) fecal pellet sampled from the Longhushan Nature Reserve, Southwest Guangxi, China. The results indicated that cortisol was negatively correlated with ambient temperature and sunlight, whereas T4 was positively correlated with sunlight. Cortisol levels sampled from spring...

Zaib Ullah1*, Sajid Mahmood2,3, Zafar Iqbal4, Fakhar-i-Abbas5, Naveed Akhtar1 and Abdul Majid Khan6*

... carried out by transect method, with 18 transects were in Kaghan and 15 transects in Siran Valley. A total of 1858 signs were recorded during the field survey; among these 1213 dig marks, 186 plants uprooting and two setting places. An average of 49.95 signs/km2 was recorded; this is a very high encounter rate. According to BBC (British Broadcasting Corporation) Science and Nature, the home range size of Asiatic black bears is 10 to 20 km2, so we concluded th...

Erni Damayanti1,2, Herry Sonjaya2*, Sudirman Baco2, Hasbi Hasbi2, Ekayanti Mulyawati Kaiin3

...measured using the ELISA method. The results showed that there were 228 developing embryos (41%) after 48-hour of culture with 2-, 4-, 8-, and 16-cell division. EGF concentrations in the medium of 2-cell to 16-cell ranged from 0.8539 ng/mL-0.2838 ng/mL. The concentration of FGF2 in follicular fluid is 0.629 ng/mL, while the concentration of FGF2 in maturation media, culture media 2 cells, 4 cells, 8 cells and 16 cells respectively, namely 0.032 ng/mL, 0.015 ng...

Mudawamah Mudawamah1*, Sumartono Sumartono1, G. Ciptadi2, E. Susanto3, Y. Hartoyo4, L. Affandhy5,6

...ir crosses. The research method was a field study with quantitative and laboratory analysis, with statistical analysis using ANOVA and LSD with SPSS Statistic 9 software. The research design used descriptive with total sample of 60 heads (morphometry and physiological status) and 24 heads (total protein).  There are three breeds of sheep: Sapudi, Dormas (Dormer x Sapudi), and Suffas (Suffolk x Sapudi). Sapudi, Dormas, and Suffas Ewes with only one child e...

Jameel-ur-Rehman1, Asghar Ali Kamboh1*, Ahmad Ali Moryani1, Riaz Ahmed Leghari1, Abdullah Sethar2, Ali Raza Nizamani3, Abdul Ahad Soomro3, Kanwar Kumar Malhi1 

...ing conventional culture method and isolates were tested for antimicrobial sensitivity according to CLSI method. According to results, the prevalence of bacterial organisms was high (p < 0.05) in CC than CM and LR. E.coli were the most common organism in market and pond fish samples with occurrence of 86.6% and 66.6% respectively. Next most prevalent pathogens were Salmonella spp. (78.3% & 58.3%), Shigella flexneri (6...

A.M. Abdul Azeem1, A.N. El shahat1* and Mohamed H.M. Abd el Megid2

...to physical and chemical methods because it is inexpensive and considered environmentally friendly. Therefore, the objective of this study was to synthesize selenium nanoparticles from Citrus paradise peels extract (SeNPs-CP), their general characterization, and their assessment for antioxidant activities against liver injury induced by bisphenol A (BPA) in male rats. In this work, Citrus paradise peels extract used for reduction of sodium selenite (Na2SeO3) t...

A.M. Abdul Azeem1, A.N. El shahat1* and Mohamed H.M. Abd el Megid2

...to physical and chemical methods because it is inexpensive and considered environmentally friendly. Therefore, the objective of this study was to synthesize selenium nanoparticles from Citrus paradise peels extract (SeNPs-CP), their general characterization, and their assessment for antioxidant activities against liver injury induced by bisphenol A (BPA) in male rats. In this work, Citrus paradise peels extract used for reduction of sodium selenite (Na2SeO3) t...

Wei Wang1, Meifeng Zhou2, Yuebo Liang1, Fan Zhang1, Zhong Wu3, Shaowei Mo4* and Yi Qing Wang1*

...the clinical therapeutic methods of HER2 positive breast cancer.

...

Stepanus Pakage*, Daud Krey, Trisiwi. Wahyu Widayati, Deny Anjelus Iyai, Agustinus Gatot Murwanto, Alnita Baaka, Daniel Yohanes Seseray, Djonly Woran 

...n Manokwari Regency. The method used is the Ordinary Least Square (OLS) and Maximum Likelihood Estimation (MLE) methods on the Cobb-Douglas production function model and the Technical Efficiency Effect Model option. The results showed that piglets, feed, and firewood had an effect on pig production. Pig farmers in Manokwari Regency have been allocatively efficient, but technically and economically inefficient. 

...

Arnold Christian Tabun*, Ferdinan Suharjono Suek, Cardial L Leo Penu, Johanis A. Jermias, Thomas Lapenangga  

...rome b (CYT B) gene. The methods used CYT B gene base methods of DNA analysis such as DNA extraction, Polymerase Chain Restriction, DNA sequencing, and identification of DNA sequences nucleotide. Bali cows with sorrel, black and white coat colors are used with an age range of 3-5 years. DNA sequencing conducted multiple alignments, the identification of the genetic distance, and the preparation of phylogenetic trees using th...

Nadeem Akmal1, Abid Hussain1*, Muhammad Yousaf2, Waqar Akhtar1 and Hassnain Shah1

...conventional rice sowing methods in the rice-wheat cropping zone of the Punjab province has been made by applying economic analysis technique. As, Basmati-Super and Basmati- 386 are main rice varieties grown in the country. Thus, this study is based on data of 46 purposively selected farmers for the crop season 2021, including 30 and 16 farmers of Basmati-386 and Basmati-Super, respectively. Cost comparison of the crop produced through mechanical and conventio...

Bdour Muhammed Al-Shweily1*, Jawad Bulbul Al-Zaidawi2, Muhammed Jubair Hanawi1 

...aken to identify optimal methods for its control. Due to the adverse effects of chemical pesticides on consumer health, particularly human health, the pursuit of alternative safe measures has been paramount. Among the prominent alternatives, biological control has garnered attention, wherein environmentally safe organisms such as nematodes and fungi are employed. This study examines their efficacy in controlling the insect larvae and pupae. The integration of ...

Muneer Abbas1*, Sohail Abbas2, Imran Faraz3, Niaz Hussain1, Muhammad Aslam1, Muhammad Irshad1, Mudassar Khaliq1, Abdul Ghaffar1, Zubeda Parveen1, Muhammad Nadeem1, Sana Ullah4* and Malik Akhtar Iqbal5

...: justify;">Conventional methods of insect control have faced significant challenges, primarily due to the extensive use of pesticides. This overreliance on pesticides has led to issues such as pest resistance and environmental pollution. Some formerly minor pests have transformed into major threats by altering their biology and host preferences. Integrated Pest Management (IPM) offers a promising solution by reducing the reliance on insecticides. Many countri...

Khwaja Junaid and Shah Alam Khan

... safe and cost-effective method for the control of (RLF). Keeping in view the importance of the pest and economic value of the crop this experiment is designed to screen thirty five genotypes for resistance against rice leaf folder. These genotypes were evaluated for resistance against RLF in green house in the Department of Plant Breeding and Genetics, Agriculture University Peshawar, by following the method of Heinrichs et...
Akram Ali Baloch1*, Adeel Ahmad2, Kaleem U. Kakar3, Sara Naudhani1, Samiullah Khan1, Agha Muhammad Raza3, Imrana Niaz Sultan1, Humaira Zahid4, Saadullah3 and Shakeela Daud1*
...s performed by inorganic method. DNA was amplified by polymerase chain reaction and subsequently sequenced to confirm any genetic variability in the affected individuals of the families. As a result, two different missense mutations (c.830C>A resulting p.Ala277Glu and c.332C>T resulting p.Pro111Leu) in affected individuals in two of the families were identified. The nonexistence of mutations in EPM2B in the other two families could be due to presence of ...

Razia Sultana1, Shinawar Waseem Ali1*, Ghulam Murtaza2 and Shahid Mahmood2

...red traditionally by the method of back-slopping. Recently, it is also prepared commercially by using bacteria in different combinations. In this study, we aimed to detect and identify bacteria present in locally processed yogurt using the advanced next-generation sequencing (NGS) method. Yogurt samples were collected from open-air shops located in different areas of Pakistan. All yogurt samples were mixed to make one compos...

Rimsha Ijaz, Rida Zahra, Naseer Ali Shah*, Haroon Ahmed, Nazish Bostan and Sadia Sattar

...ified through genotyping method. In the HCV positive sample, genotype 3a was the most common genotype, accounting for 652 (76.3%) of the infected people. Given the enormous morbidity and mortality caused by HCV, having precise detection methods that allow for early diagnosis and care for better outcomes is critical.

...

Muhammad Haseeb Malik2, Muhammad Moaeen-ud-Din1*, Ghazal Kaukab Raja2 and Feroza Hamid Wattoo2

...ment length polymorphism method. Eleven markers were developed that were converted to single nucleotide polymorphism markers for high throughput genotyping. The allele frequencies in both breeds were determined for discrimination ability using AFLP. The probability of identifying Sahiwal breed was 0.86 and probability of misjudgment was 0.021 using single selected marker. However, probabilities for judgment and misjudgment with two markers and combined with th...

Wei Fang1,2,3, Jiawei Hong1,2,3,4, Zhengyi Fu1,2,3, Jing Hu1,2,3, Gang Yu1,2,3, Zhenhua Ma1,2,3* and Humin Zong5*

...gene, and bioinformatics methods were then adopted to perform functional annotation classification, metabolic pathways and SSR feature analysis. The results showed that a total of 42.40 Gb data were obtained from transcriptome sequencing, and 38,817 Unigenes were obtained after assembly and de-redundancy; Using BLAST to annotate the assembled unigenes with seven functional databases (NR, NT, GO, COG, KEGG, Swissprot and Interpro), a total of 23,417 unigenes we...
Muhammad Abdul Mannan1,2, Sharmin Chowdhury2, Md Abul Hashem3,4 and Md. Hazzaz Bin Kabir1,5*
...ological and microscopic methods. The Polymerase Chain Reaction (PCR) was then employed to amplify the sec internal transcribed spacer (ITS-2) of the nuclear ribosomal DNA and the mitochondrial nicotinamide dehydrogenase subunit 4 gene (nad4) to identify H. contortus, followed by direct sequencing. Out of the 400 samples tested, 186 (46.5%) were positive for H. contortus. The parasite was found in 51.3% of sheep and 43.6% of goats. Through the sequencing analy...

Liyun Chang*, Yazi Li, Yumei Cai and Chenghui Li

...ed a novel multiplex PCR method for detection of the aforementioned three pathogens. The results show that the amplified fragments of interest were 280 bp, 151 bp, and 111 bp for BVDV, BRV, and BCV, respectively. The method had no cross-reaction to Escherichia coli, Salmonella, and infectious bovine rhinotracheitis virus. Moreover, it detected the minimum limit of 1.19 × 103 copies/μL for BVDV, 3.89 × 102 copi...

Muhammad Altaf1*, Arshad Javid2, Abdul Majid Khan3, Sadia Nazer4, Irfan5, Khalid Javed Iqbal6, Muhammad Sultan Haider7 and Sana Ashraf7

...ted through linear count method viz., direct observation (personal count and record voices) and indirect observation (presences of carcasses, fecal pellets, pug marks and meeting with local communities). The habitat preferences of large, medium and small mammals varied significantly. A decrease in mammalian diversity was observed from forest habitat to urban landscapes. Indian wild boar, Asiatic jackal, Indian fox, jungle cat, Indian pangolin and long-eared de...

Ihab G. AL-Shemmari1*, Ali Hussein Fadhil1, Mohammed Assad S. Alkabi1, Eman Jawad Jabber2 

...ts, suggesting that this method might be employed as a rapid regular screening test. 

...

SHEHLA AKBAR*, SAIQA ISHTIAQ*, KHALID HUSSAIN, ANS MUNIR & SAIRA REHMAN

...iv>
using standard methods for estimating the primary and secondary
metabolites. The maximum value of total carbohydrates (11.04μg/ml),
total starch (14.69μg/ml), total protein (14.34μg/ml), total amino
acids(28.43μg/ml), total lipids (3.67 μg/ml), total glycosaponins (14.95%),
total alkaloids (24.67%), total polyphenolics (28.40%) and total flavonoids
(5.67%) were found in given ...

MUHAMMAD ASLAM*, ASAD GULZAR, AMINA ASGHAR, MISBAH IRSHAD, ZAHRA NOREEN, SADAF JAVAID, ABRAR HUSSAIN, UMAIR AHMAD & SAMREEN KOUSAR

...se UV spectrophotometric method by using 0.1N HCl as a
solvent, for the validation of levofloxacin in injection form. The λmax for
levofloxacin was found to be 293nm using 0.1N HCl as a blank.
Calibration curve data proved that the proposed method was linear in
the concentration range of 3 to 7μg/mL. The proposed m...

KHADIJA SUMMIA*1, ROHEELA YASMEEN*2, ALEEM UN NISA3, SAMIA DJEFFAL4 & TAHIRA JABEEN1

...yer chromatography (TLC) method. The results showed that out of 50 samples, 20 (40 %) were fit as no level of aflatoxins detected in these samples. However, 30 (60 %) were positive for aflatoxins. 15(50 %) samples were highly contaminated and unfit. However, the other 15 (50 %) samples were within the tolerance range of aflatoxins. Aflatoxin B1 was detected in all 30 samples and aflatoxin B2 was noticed in only 10 samples. However, Aflatoxin G1 and Aflatoxin G...

Salam N. Aritonang*, Elly Roza, Rizqan, Winda Habsani 

...ths of age. The research methods used census and survey methods with purposive sampling techniques for determining the research location. We observed variables such as body weight, chest circumference, body length, and shoulder height. Data analysis was carried out descriptively based on arithmetic mean, standard deviation, and coefficient of variance. The result showed that the body weights of male and female buffalo crosse...

Orooba Mohammed Saeed Ibrahim1*, Rawaa Saladdin Jumaa2, Nibras Zeyad Yahya1 

...ion, agar well diffusion methods and time kill curve. Results revealed that the used juice extracts were variously bacteriostatic and bactericidal against the examined bacteria depending on concentration. Phytochemicals analysis indicated the presence of alkaloids, phenol, saponins, flavonoids, tannins, and glycosides. The test organism was more sensitive to C. limonum compared to C. aurantium. The MICs ranged from 1.56 to 12.5mg/ml; MBCs, (3.125 - 25mg/ml) an...
Fariha Javaid1*, Zahoor Qadir Samra1, Madeeha Shahzad Lodhi1,2, Aroosha Hussain1 and Gulnaz Pervaiz1
...t-effective purification methods. This research work aims to purify BRM by using magnetic nanoparticles. After purification, BRM enzyme was characterized and utilized in the detergent industry due to its stability at a wide range of pH and temperature; results showed that stem BRM has the highest enzyme activity at 23.5 U/ml as compared to crown leaf (11.7 U/ml) and peel (19.5 U/ml). The specific activity of leaf, peel, and stem BRM was determined 0.8, 1.2, an...
Ummul Firmani1,3, Rahmi Nurdiani2, Arning Wilujeng Ekawati2 and Happy Nursyam2*
...sed on the well-diffused method against Aeromonas hydrophila showed that B. paramycoides did not produce an inhibition zone around the bacterial wells. The molecular identification found that the bacterial species was B. paramycoides B2.1. These results suggested that Bacillus paramycoides B2.1, which is found in the digestive tract of milkfish, can be used as a probiotic candidate for fish feed indicated by several probiotic tests that have been carried out.<...

Urosevic Milivoje1, Matejevic Milosava2*, Mandic Radomir3 and Dameski Pance4

...flon trophies. Using the method of descriptive statistics, the mean values ​​of the length of the left and right horns were obtained. Results showed that the right horn was slightly more developed in the observed population. Namely, the mean value of the length of the right horn is larger, while the mean values ​​of the circumference of the left and right are almost the same. The results of Pearson’s correlation showed a statistically significant...

Vijay Kumar1, Asghar Ali Kamboh1*, Nazar Ali Korejo1, Ambreen Leghari2, Faiz Muhammad Khand2, Riaz Ahmed Leghari1, Khaliq-ur-Rehman Bhutto3 

...ing to ISO 6579 standard method. Identification of isolated bacteria was done using the Salmonella specific biochemical tests. Antimicrobial resistance patterns were determined using the disk diffusion method. Salmonella were detected in 22, 19 and 10 samples, showing prevalence of 40.0, 42.22 and 40.0 percent in broiler, layer and ducks, respectively. Duck isolates exhibited 100 percent resistance against tetracycline, sulf...

Muhammad Shahid Nisar1*, Ahmad Kamran Khan1, Ghulam Fatima2, Irfan Ahmed4, Khizar Sami Ullah2 and Syeda Amber Hameed2,5

...sing complete randomized method consisting Diet 1 (D1) (treatment 1) with 100% basal feed (BF) and 0% fruit waste (FW), diet 2 (D2) (75 BF + 25% FW), diet 3 (D3) (50% BF + 50% FW) and diet 4 (D4) (25% BF + 75% FW). Non-significant results (p>0.05) were found in feed intake and body weight gain and carcass characteristics in all treatment groups while feed conversion ratio (FCR) were resulted significantly difference (p<0.05) in D2 (1st, 3rd, 4th, 5th wee...

Putu Henrywaesa Sudipa1*, Hapsari Mahatmi1, Ketut Tono Pasek Gelgel1, I Gusti Ketut Suarjana1, I Nengah Kerta Besung1, Romy Muhammad Dary Mufa2 , I Wayan Sukernayasa3 

...i by using morphological methods. The samples were vaginal swabs obtained from 22 healthy female Bali cattle from various locations in Bali, Indonesia. For isolation, vaginal swabs were cultured on Sabouraud’s dextrose agar (SDA) medium and identification was performed macroscopically by observing colony growth and microscopically using the tape smear method stained with Methylene Blue stain. The results showed that 32...

Anshara Javed Qureshi and Ishrat Aziz*

...icroscopy and floatation method. In this study, 18 (11 males and 7 females) out of the 100 samples (with a prevalence 18%) were found infected with Capillaria spp. of nematodes. The qualitative examination also revealed that the Capillaria spp. of nematodes was more prevalent in males (36.67%) than females (10%). This study will be helpful in raising awareness among pigeon owners for better control and treatment strategies for capillariasis and also to improve...

I Nyoman Sumerta Miwada1*, I Nyoman Sutarpa Sutama1, Agus Susilo2

...and 8%(P4). The research method used one-way analysis of diversity (at p<0.05) and continued with the Duncan test using the SPSS version 25 system. The results showed that increasing the level of Moringa leaf extract had no effect on pH, water content, and fat but significantly affected protein and ash content (p<0.05). In the color aspect, the L* and a* values ​​significantly decreased (p<0.05), but the b* values ​​significantly increased. Te...

Roberto Gallegos-Acero1, Ali William Canaza-Cayo2,4*, Francisco Halley Rodríguez-Huanca1, Rubén Herberht Mamani-Cato3, Abigail de la Cruz1, Júlio Sílvio de Sousa Bueno Filho4 

... were analyzed using the method of genealogical analysis method. ENDOG program v.4.8 was used for the calculation of the following parameters of the genetic structure such as: average inbreeding coefficient (F), average relatedness coefficient (AR), the effective numbers of founders (), effective number of ancestors (), generation interval (GI) and the pedigree completeness, the ENDOG program v.4.8 was used. The F and AR wer...

Tevina Edwin, Arfa’i*, Olivio Dickresna

...he study. We used survey methodology. Data were collected using questionnaires and interviews. respondents in this study were 97 breeders. The cattle kept were generally Peranakan simental (F1-F4), Pesisir cattle, Bali cattle, and Peranakan Ongole cattle. The feed provided was forage and concentrate with a rate of 10% of the cow’s body weight. Cattle were intensively reared in a semi-permanent housing system. Disease prevention has been done well, especi...

Tanvir Hussain1,2*, Kafeel Ahmad2, Muhammad Bilal Zia1, Hammad Uddin1, Khalid Hussain1, Muhammad Inamulla Khan3 and Said Akhtar Khan1

...d polymorphic (RAPD) DNA method. Five study sites were selected and 20 samples (needles) from each site were collected. Six RAPD markers produced 92 amplicons with sizes range 250-2000bp. Among all studied populations 100% polymorphism was observed. The highest percentage of polymorphic bands (PPB=82.61%), observed number of alleles (na=1.82), effective number of alleles (Ne=1.34), Shannon index (S=0.33) and Nei,s genetic diversity ( He=0.21) were scored for A...

Imtiaz Khan1*, Abdullah1, Muhammad Ibrahim1, Muhammad Ishfaq Khan1, Saima Hashim1, Shomayela Afzal2 and Khalid Nawab3 

...appropriate weed control methods to enhance crop productivity and reduce weed competition in chickpea cultivation. However, it is essential to consider factors like herbicide resistance, environmental impacts, and sustainability while formulating weed management strategies in agricultural practices. Further research may be needed to optimize the application of different treatments and to explore additional weed management techniques to maintain effective weed ...

Wenxiao Jia1*, Yilinuer Yilihamu1, Yunling Wang1, Shuang Ding1, Dilinuerkezi Aihemaiti1, Hanjiaerbieke Kukun1 and Yanhui Ning2

...bility of a new combined method of constructing an unstable atherosclerotic plaque model in the abdominal aorta of New Zealand rabbits. The experimental New Zealand rabbits were fed adaptively for one week before being punctured through the abdominal aorta with a balloon to injure the intima. Vitamin D (1.5 mL/kg) was administered one week after the surgical model was completed. Following injection, the animals were fed a high-fat diet for 16 weeks. High-resol...

Kristina Morkūnienė*, Rūta Insodaitė, Laimutis Kučinskas, Renata Bižienė 

...olymerase chain reaction method. Statistical analysis was performed with SPSS Statistics 20 and PLINK software. SNP at position 7454459 (Asn → Thr) of Mblk-1 gene mutant allele A was more common in untreated domestic and wild honey bees (18.90% and 8.14% respectively; p=0.001) compared to treated domestic bees which persistently infected with the disease. Regression analysis showed that recessive AA genotype of this polymorphism significantly reduced the ...

Frah R Kbyeh1*, Ahmed N. Abedsalih2 

...uting factors is a novel method to overcome this scarcity. The analgesic medication diclofenac was reported to have anti-virulence action against resistance Escherichia coli O157 :H7. This study aimed to demonstrate the antimicrobial effect of diclofenac, Ciprofloxacin, and a combination (diclofenac +ciprofloxacin) against clinical resistance E coli o157 H7 isolates by using  Polymerase chain reaction  (PCR). The presented study, all rabbits, forty-...

Hafiza Mehwish Iqbal1, Salman Khurshid1*, Saqib Arif1, Qurrat-ul-Ain Akbar1, Saba Iqbal2, Shahid Yousaf3, Kainat Qureshi1, Abdul Karim Khan5, Abdul Ahad6, Aqeel Ahmed Siddique4 and Neelofar Hamid7

...tudied by Well diffusion method. Three solvent extracts Aqueous, Acetone and Methanol with concentrations (5,10,15 and 20%) was prepared. Our study showed that maximum zone of inhibition (ZOI) was observed against bacteria for M. Koenigii i.e., 22mm and 23 mm for fungi, while; O. basilicum and S. asoca plants have21 and 22mm MIZD. Methanol 20%has maximum antioxidant activity in O. basilicum whereas, in S. asoca and M. koenigii 20% aqueous showed maximum activi...

Muhammad Nauman Arif1, Muhammad Mansha1* and Tanveer Hussain2

...y using in-house organic methods. PCR was used to amplify a total of 880 bp of overlapping genes, which were then sequenced. Bioedit software was used to align, and edit the sequences, and single nucleotide polymorphisms (SNPs) were evaluated. The MEGA X-based maximum likelihood method was employed to study the genetic relatedness between sequenced samples and reference sequence as well as other deer. Among query sequences, ...

Shudong Liu1,2, Shuai Wang1,2, Haoran Cui1 and Genyuan Chen1,2*

...urated phenol-chloroform method. The DNA was genotyped using the Illumina Ovine SNP50 Bead Chip. After quality control, we detected 4,8335 SNPs, which included four SNPs that are significantly associated with the ACMS of sheep. We identified four quantitative trait loci (QTL) regions for ACMS. These QTLs on Ovis aries (OAR) 2 and OAR26. We observe genome-wide significant association with ACMS at four genomic loci: OAR2_130068033.1, OAR2_216769207.1, OAR2_12828...

Aniqa Nasir1, Rashid Masih1, Shazma Massey1*, Laiba Arshad2, Sabi Ur Rehman2, Irva Waqar1 and Anwar Khalid3

...advanced isoconversional method. Isoconversional method is used to determine kinetic triplet including activation energy, pre-exponential factor and mechanism of thermal degradation. The prepared dressings were subjected to thermal analysis. Thermogravimetric analysis showed that the degradation of wound dressing occurs at 225˚C - 400˚C. Thus, the dressing was found to be stable up to wide ranges of temperature. Kinetics o...

Sabi Ur Rehman1*, Laiba Arshad1, Saman Ali1, Shazma Massey2, Safeer Khan3, Abdul Samad4 and Fazal-Ur-Rehman5

...esents a green synthesis method using Sarcococca saligna leaf extracts to successfully produce both silver and gold nanoparticles. The formation and stability of the nanoparticles were verified through a change in physical colour and characterized using Ultraviolet-Visible spectroscopy and Scanning Electron Microscopy. Antibacterial and antifungal activity assessments revealed moderate efficacy against Candida albicans, Staphylococcus aureus and Escherichia co...

Muhammad Mansoor1, Shahid Hameed Khalil2,4*, Muhammad Arshad Khan3, Ghani Akbar2, Muhammad Shahzad Khan4, Rana Naveed Mustafa2 and Salah ud Din5

...act of varied irrigation methods on mung bean growth, yield, and nodulation. Results showed superior performance in raised beds compared to flatbed systems, indicating their positive influence on mung bean outcomes. The study highlighted that a three-day irrigation schedule positively affected growth and nodulation, while a six-day interval was optimal for maximizing seed yield. Balancing vegetative growth and seed yield is crucial for overall productivity in ...

Muhammad Luqman1*, Muhammad Talha Shoaib1, Muhammad Yaseen1, Umair Safdar2 and Hassan Raza2

...A cross sectional survey method using convenient sampling was adopted for this research and data was collected from 300 respondents with the help of structured interview schedule. The results revealed that all the respondents (100%) considered ‘enhance farm production’, ‘control of insect/pest’ and ‘weed eradication’ as major factors for application of pesticides. Moreover, results also indicated that 66% of respondents were...

Kanda Yanuar Muhamad1,3, Trioso Purnawarman2, Chaerul Basri2* 

...ng the snowball sampling method. Data was collected through structured questionnaires administered during interviews. According to the research, most employees (63%) working in reptile import and sales industries have excellent knowledge about zoonoses-assosiated with imported reptiles, while about one-third (34%) have moderate knowledge, and a small fraction (4%) have poor knowledge. However, there are still misconceptions and gaps in understanding, particula...

Sidra Hafeez1, Tayyaba Sanaullah2, Hafsa Naeem3, Mah Noor Hassan4, Muttalib5, Farhana Kausar6, Muhammad Salman Hameed7*, Muhammad Anayat Ullah8, Abdul Samad9, Memoona Bashir10 and Sadaf Shabbir11

...test was performed. CTAB method and Internal Transcribed Spacer region were applied to isolate and amplify the fungal genomic DNA respectively, by using universal primers ITS1/ITS4. The analysis of polygenetic of an amplified product revealed that black rot caused by D. bryoniae in pumpkin.

...

Adeel Kazam1, Safdar Sidra2, Zulfiqar Ali1*, Rida Ahmad1,3, Ahmad Bilal1 and Aliza Batool1,3

...unt) as well as indirect method (interviews) from December 2020 to May 2021. In total, 139 avian species of 27,450 individuals were recorded at study sites. Results revealed that the species richness was maximum at Uchalli (133), followed by Khabbaki (92), Jahlar (88), and Ahmedabad lake (79). The Shannon Weiner index and Simpson index values for Jahlar lake, Uchalli lake, Khabbaki lake, and Ahmedabad lake were (2.99, 0.90), (2.32, 0.82), (2.26, 0.66) and (1.7...

Ismail Demircioglu1, Yasin Demiraslan2, Barıs Can Güzel3*, Ali Koçyigit4 and Aysegül Demircioglu5

...ng geometric morphometry method through their crania. For this purpose, heads of 7 adult Hamdani sheep and 10 adult Awassi sheep were used for the dorsal analysis of cranium and heads of 8 adult Hamdani and 9 adult Awassi sheep were used for lateral analysis of cranium. In the dorsal and lateral comparison of the breeds, the first principal component (PC1) explained 41.905% and 39.078% of the total shape difference, respectively. When the procrustes coordinate...

Siswanto Imam Santoso*, Agus Setiadi, Maulina Syafa’ati Ningrum 

...s research uses a survey method. The sampling method in this study was total sampling or census with a total of 6 farmers. The sample was divided into two levels: strata I 1-30,000 broilers and strata II > 30,000 broilers chicken. The data analysis method used was descriptive statistical analysis. Data were analyzed using the formula for production costs, total revenue, and profitabilit...

Khansa Jamil1, Muhammad Ramzan Khan1, Asad Jan2 and Ghulam Muhammad Ali1

...ed out by well diffusion method. A maximum number of compounds were eluted by methanolic extract by thin-layer chromatography and a total of 70 compounds were identified by GC-MS analysis. The phytochemical screening showed that the methanolic extract of the whole plant was rich in alkaloids and tannins. Zones of inhibition around the bacterial colonies show the antibacterial activity of the plant. Extract of the targeted plant was effective against all test o...

Adnan Ibrahim Khamis1, Jasim Mohammed Saleh2*, Najwa S. Ali3 and Anwer Thamer Ghaffoori2

...tural life. The research method is to use the questionnaire to achieve the objectives of the study after it has been corrected according to the opinions of experts in the required field. The research sample included (228) respondents distributed over (11) agricultural divisions. A descriptive approach was used as a method to achieve the research goals. A questionnaire was designed to collect data for the purpose of the study...

Merita Ayu Indrianti1,2*, Didi Rukmana3, Eymal Bahsar Demmallino3, Muh. Hatta Jamil3

...esearch used qualitative methods with a literature study approach supported by data from the Head of the Gorontalo District Agriculture Office as key informants. The results of this study indicate that the Gorontalo regency government currently has a regional regulation (PERDA) No. 2 of 2017 which regulates LP2B protection program, however, there are no clear geographical boundaries regarding the LP2B program in Gorontalo Regency also not implemented yet the r...

Abida Mushtaque1, Ali Ahmad Sheikh1, Aamir Ghafoor1*, Wasim Shehzad2 and Nadeem Ahmad3

...eat shock transformation method to get expression. The product size was confirmed by restriction digestion. The rOmpH protein was purified by immobilized metal affinity chromatography by using His-Ni resins to get desired protein and characterized qualitatively by SDS-PAGE by using His antibodies and western blotting. Once purified the immunogenicity of rOmpH was evaluated in BALB/c mice. The expression of recombinant OmpH protein (rOmpH) resulted in its accum...

Wei Wan1, Xiao-Li Wang1, Shu-Juan Wang2, Qin Si2 and Li-Qing Duan1*

...e modified residual film method was used to test the toxicity of individual and binary mixture of these monoterpenoids against adult P. sinica. The effects of these monoterpenoids on acetylcholinesterase (AChE) and glutathione S-transferase (GST) activities in P. sinica were assessed in vitro. Correlation between numbers of synergistic or antagonistic binary mixtures and AChE or GST activities was analyzed. The results showed that 2-ethylimidazole had the stro...

Dan Song1,2, Ming-Juan Ge3, Jie Li1,2, Yi Jiang1, Xiu-Mei Kong1, Jiao-Jiao Xu1, Xu Ji1,2, Rui-Xin Shi1 and Qin Zhao1,2*

...l extract. Three extract methods were employed to evaluate the best extraction methods. Male Kunming specific pathogen-free (SPF) mice were randomly divided into blank control, model (hypoxia), positive (propranolol 30 mg/kg + hypoxia), and three G. straminea ethanol extracts dose groups (10, 5 and 2.5 g/kg respectively + hypoxia), administered intragastrically once a day for 14 consecutive days. After that, multiple hypoxia...
Qingsen Ran1,2, Manjing Li3, Jiayin Han3, Lifang Wang3, Han Wang3,4, Shaobo Liu5* and Yanping Wang1*
...gression models. The K-M method was used to determine the difference of OS rate with high and low risk scores. The accuracy of risk scores were determined based on receiver operating characteristic (ROC) curve. Hierarchical analysis and Cox regression were performed to assess the correlation between CC risk score and clinical symptoms. Moreover, qPCR was used to verify the expression level of prognostic genes in human colorectal cancer cell line (HCT116) and h...
Mahmoud M. Bayoumi
...production, the delivery methods, and the essential compositions added to the mRNA vaccines to enhance efficacy and stability. This information ultimately would pave the way to better thinking to combat viral infectious diseases.
...
Moustafa A. Zaghloul1*, Mohamed F. Azooz1, Saleh E. Ali1*, Heba M. Soliman1, Maha M. Sayed1, Mohamed H. Kafafy2 and Alaa R. Morsy1
...V. Several bioinformatic methods were employed to find superior epitopes. Fifty-three epitopes passed the three B-cell prediction tools. Twelve promising and top linear B epitopes passed the antigenicity, allergenicity and toxicity tests. Fifty-seven MHC I epitopes were the most promising, while (49) were from MHC II. Thirteen peptides for MHC II were chosen as the best targets for the vaccine. Seventeen MHC I epitopes were the most promising because they were...
Amany M. Reyad1*, Aya Maher Rabie1, Reda Mohamed Taha1 and Khalid El-Dougdoug2
...ntages over conventional methods of controlling pathogenic bacteria in which they have a high ability to self-reproduce, host specificity, and develop with their bacterial hosts. The most common pathogenic bacteria were isolated from fresh meat and identified using the VITEK II automated system. Phages specific to Escherichia coli, Staphylococcu...

Zhimin Wu1,2, Yaping Yang1, Arshad Zahoor1, Aftab Shaukat1 and Ganzhen Deng1,2*

...ut different measurement methods and indexes, the simple radiographic findings are difficult to be used in accurate clinical evaluation. This test aimed to develop a radiographic method of comparing canine posterior lung field angles include vertebrophrenic angle (cranial), vertebrophrenic angle (caudal), sterno-diaphragmatic angle, costophrenic angle (left), costophrenic angle (right) to identify whether pulmonary disordere...

Lijiao Wang1, Haibin Chen2*, Hongyan Yu1, Zexian Fu3 and Jianjun Zhao2*

... and immunohistochemical methods were used to detect the expression of EPAS-1 in the three groups. The expression of EPAS-1 and the clinicopathology of RCC were further analysed. To examine the relationship between these characteristics, a receiver operating characteristic curve (ROC) was used to evaluate the diagnostic value of EPAS-1 expression for lymph node metastasis in RCC. Compared with the adjacent tissue, the expression of EPAS-1 in RCC was significan...
Rabiea Pervaiz, Shaista Bano*, Sarfraz Ali Tunio, Abdul Nabi Jatt and Aisha Amber Soomro
...d by screening/selection method and designated as B. pseudomycoides strain SB138m. The sequence of 16S rRNA gene of both wild type and mutant strains of B. pseudomycoides was comparable. In vitro test for antagonism of B. pseudomycoides strain SB138m against indicator cells showed that the strain contained antibacterial potential. The present study shows inhibitory spectrum of B. pseudomycoides SB13m with conclusion that Bacillus species from a diverse environ...
Muhammad Fiaz Qamar1*, Tahir Hussain1, Iram Liaqat2, Madiha Kiran1, Abdur Rahman Ansari3, Faiza Yasmeen1 and Tahira Batool3
...rough routine laboratory methods in district Jhang. Fresh fecal samples (n = 1800) of pigeons were gathered in plastic bags from district Jhang home fanciers and local retailers. Smearing, sedimentation, and flotation procedures were among the various parasitological methods used to analyze all of the samples that were gathered. Herein, the prevalence of coccidiosis was 70.7% and that of capillariasis was 15.7% wherea...
Budi Utomo1*, Amaq Fadholly2,3, Endang Tri Margawati2, Alek Ibrahim2, Ristaqul Husna Belgania1, Muhammad Fajar Amrullah1, Rimayanti1 and Hermin Ratnani1
... of Madrasin cattle. The method used in this research was a descriptive analysis by duplex polymerase chain reaction and analyzing the results of the SRY gene sequencing of limousine cattle and Madrasin cattle. In the present study, we showed that the SRY gene of Madrasin cattle and Limousine cattle could be amplified with SRY F and SRY R primers with a PCR product length of 318 bp. Based on these results, Madura, Limousin, and Madrasin cattle had similarities...
Nauman Jamil Khan1, Rabia Tariq2, Hina Saleem2 and Muhammad Waheed Mushtaq2*
...troscopy, chromatography methods, ion selective electrode method, laser-induced breakdown spectroscopy, complexometric titration method and thermometric endpoint titrimetry are developed to determine the quantity of sodium. Spectrophotometric methods are cheap, easy to handle and more precise to quantify sodium in food contents.
...

Rahmat Elahi1, Gulnaz Parveen1*, Nazara1, Faryal Ali2, Nain Tara3 and Saba Iqbal1

...tter test and agar plate methods, a total of eight different species of fungi were isolated from five different maize varieties. In the blotter test method, only spores and mycelium of seed-borne fungi were detected, that was further confirmed by the agar plate method revealed the presence of eight different types of fungal colonies in maize varieties, each with varying percentages. Like T...

Adnan I Khamis1, Jasim Mohammed Saleh2*, Najwa Ali3 and Anwer T Gaffoori2

...e approach was used as a method to achieve the objectives of the research. A questionnaire was prepared to collect study data if it included two main axes. The first axis included (8) fields of developing and developing the capabilities of rural women by (50) paragraphs. The second axis included the determinants that hinder Work in agricultural projects, as it included four areas with (22) paragraphs. After the process of data collection and unpacking, the sta...

M. Azalou1,3*, C. C. Kpomasse1, A. S. Assani3,4, I. T. Alkoiret3,4, Wéré Pitala1,2  

...(p<0.05). The farming method and the total number of birds varied between rearing groups (p<0.05). The identification of these animals through their size and silhouette was more noticeable in Group 3 (58.62%) than in Groups 1 (46.34%) and 2 (50%). The production objective of all farmers was the production of meat for consumption. All geese farmers face almost the same difficulties, such as diseases, mortality, theft, and predators. Goose farming in north...

Rana Mahmood Ahmad*, Orooba Mohammed Maeed  Ibrahim

...pectrophotometer (GC-MS) method revealed the presence of sixteen distinct elements, making up 100% of all known compounds in their whole. The major ingredient, eugenol, made up 49.10% of the total compounds. Oil’s median lethal dose (LD50) was 2225 mg/kg, which suggests that clove oil has a relatively low level of toxicity. Eighteen adults male Wistar albino rats, six months, weighing 200–250g, were used and randomly grouped as follows: Group 1 was...

Turrini Yudiarti*, Sugiharto Sugiharto, Endang Widiastuti, Hanny Indrat Wahyuni, Tri Agus Sartono, Maulana Hamonangan Nasution

...ternal organ weight. The method was one hundred and fifty un-sexed one-day-old chicks (DOC) were reared in an open cage. With three treatments, the study used a completely randomized design. The treatments consisted of a dose of the fungus Monascus purpureus as a feed additive (FA). These included T0 (basal diet/control); T1 (basal diet supplemented with 0.5% FA); and T2 (basal diet supplemented with 1% FA). Each treatment was repeated five times with a total ...
Johan Sukweenadhi1*, Stefan Pratama Chandra1, Finna Setiawan2, Christina Avanti2, Kartini Kartini2, Arief Koeswanto3 and Deok-Chun Yang4
...synthesis is one of many methods that can be used to synthesize silver nanoparticles. Based on a statistical analysis of the average wound area, epidermal reconstruction, and TIME-H scores, significant differences began to occur from day 12 to day 14. The average wound area, epidermal reconstruction, and TIME-H scores in the diabetic mice group that were given a dermal patch formulated with silver nanoparticles synthesized using Plantago major L. extrac...
Hagag, N.M.*•, Arafa, A.*; Shalaby, M. A. ** El-Sanousi, A. A. **and Aly, M. M. 
...Application of molecular methods such as Reverse Transcriptase polymerase chain reaction (RT-PCR) and Real time RT-PCR (RRT-PCR) as a rapid, specific and sensitive detection methods currently used in national and reference laboratories worldwide. In this study a comparison of the specificity and sensitivity between 2 different formats of conventional RT-PCR (one and two steps) and 3 different formats of RRTPCR (one step usin...

Raof*, Amal M. A.; Haleem*, Iman Y.; Aly*, Nawal M.; * *Garhy, M.M. and Hosny***, Gehan A.

... the performance of this method in different animal species and different field situations. The Prio check ELISA was also used for comparison with another commercial NSP ELISA kit (SVANOVIR). Suspected cases of Foot and Mouth disease (FMD) in cattle were noticed in some farms of Sharkia governorate. The investigated cattle suffered from oral and foot lesions associated with lameness in addition to fever in some and inappetance in others. Saliva and vesicular l...

Bakr3 *, Youssef M.; Nour El-Din3, Hanan A.; Abd EL-Wahed2, Waiel F. and Hemeidal , Aläa A.

...10 fold cross validation method. We applied the obtained models to Potato leaf roll virus (PLRV) as a case study using Epitopes Model Applier Software (EMAS) which was developed as open source software and released under general public license (GPL) to predict immunogenic peptides suitable for antibodies production. The thirty amino acids peptide which start from position 163 to position 192 got high score and match the previous laboratory studies which make i...

Soliman*, Ahmed M.; Mahmoud**, Sabry Y. M. and Dawood*, Rehab A.

...d OYDV isolates. Thus, a method of identification and detection by RT-PCR of OYDV was established. This study also aimed to obtain OYDV-free plants from infected garlic plants using cloves subjected to electrotherapy, thermotherapy, chemotherapy or meristematic dissection followed by in vitro culture. A combination treatment with electro- and chemotherapy (15 mA/10 min + 20 mg 1-1 virazol) was found to more effective on viral elimination and survival of explan...
... -351 to 1320 m. A novel method called maximum entropy distribution modeling was used for predicting potential suitable habitat for gummy bark disease in Egypt using occurrence records. The Maxent model's internal jackknife test Of variable importance showed that altitude and mean temperature of driest quarter are the most important predictors of citrus gummy bark disease-habitat distribution.

...

Nasr-Eldin1 , M. A.; El-Dougdoug2, K. A.; Othman2, B. Ahmedi , Sabah A, and Abdcl-Azizl , S. Il.

...l indexing and molecular methods. In the present study, direct tissue-printing and dot-blot on the membranes, procedures has been applied on a large scale for an initial screening of HSVd, CEVd and PSTVd in Egypt. The results showed that the tissue print assays allowed clear discrimination between healthy and viroid-infected grapevine plants than dot-blot hybridization, the number Of HSVd-infected grapevine plants were 10 plants, PSTVd were 10 plants and the C...

Khatab, Eman A.H.; Zein, Salwa N. and Ahmed, Amal A.

...on using three different methods of injections. The titer Ofthe prepared antiserum as determined by indirect ELISA with dilution of tissues 1/5 was 1/1024. Purification of the immunogamaglobulin G and conjugate of IgG with alkaline phosphatase were carried out to be used for ELISA detection. The concentrations of IgG and IgG conjugated with alkaline phosphatase were 1.0 mg/ml and 1:1000 respectively. Antiserum produced specific to BBTMV was used for virus dete...

*El-Helaly, Sahar H.; ** Ahmed, Amal A.; * Awad, M.A. and ** Soliman, A.M.'

...ed AMV isolates. Thus, a method of identification and detection by RT-PCR of AMV was established.

...

El-Sayed • l , Eman H; Mahfouze , Sherin A.; Shaltout ,A. D.; El-Dougdoug3 ,Kh. A. and Sayed1 , R. A.

...nd BMV) by using syringe method of inoculation. It was observed that, only two banana plants of the concentration 6 mg/l from 2, 4-D was found to be resistant against BMV isolate. In the same time, it is not BBTV resistance banana plants

...

Sahar A. Youssef I and A. A. Shalaby 

...th no interference. This method saves time and reagent costs compared with rnonospecific RT-PCR needs several reactions for the sarne number of tests. Using this method, jnany samples can be simultaneously analyzed and it can be used routinely för sanitary and cettification prograrnmes.
...

Sahar, A. Youssef t ; M.M.A. Al-Dhaher 2 and A.A. Shalaby 

...s found to be a reliable method, thus proving the efficiency of this method (meristem tip culture) for GLRaV-l and GFLV elimination.

...

Manal A. El-Shazly1, A. s. 2 Abdel Wahab and Salwa N. Zein3

...t serological diagnostic methods such as indirect ELISA and dot-blot immunoassay (DBIA) on nitrocellulose membranes.

...

S. A. Sidaros*, S. A. EL-Kewey*, Eman A. H. khattab**, M. M. ELsharkawy* 

...vely using two different methods. The UV absorption spectrum had maxima and minima at 260 and245 nm, respectively for the two viruses. The absorbance ratios of A and A260/280 of the combined lower bands of purified BBSV preparations were 1.19 and 1.62, respectively. Infected faba bean plants yielded up to 0.48 mg/100g tissue. The corresponding data for CABMV were 1.20&1.22 and its yield was 3.52 mg/100g of cowpea leaves. The maximum antiserum titer against...

S.Y.M. Mahmoud1 and M. Hashem2

...norate. thus, precaution methods must be taken for reducing virus transmitting to other free-virus regions. 

...

A.A.Farrag;  I.A.M. Ibrahim and, H.M. Mazyad

... molecular hybridization methods. The result showed that it is more sensitive than DASELISA and can be used for large scale detection. PCR product was cloned and sequenced. Comparison between local isolate sequence and other published sequences of the Same virus shows 79.4% - 84% homology.

...

Rana Mohammed Ibrahim1*, Haider Mohammed Ali Al-Rubaie2

...olymerase Chain Reaction methods in Baghdad city between 1/11/ 2021 and 31/3/2022. Fifty jugular venous blood samples (about 5ml) were collected. The total infection rate of Leucocytozoon spp. in the blood smears was 10% (5/50), which divided into males 6% and in females 4%, while by qRT-PCR was 4% (2/50) with an equal ratio between males and females (2%). In conclusion, it is the first diagnosed study in Baghdad city to detect Leucocytozoon spp. in broiler ch...

Sulake Fadhil Al-Zubaidi1*, Ghusoon A.A. Alneamah2, Ali Saleh Mahdi1, Abdulraheem Abduljalil Wali3

... was to evaluate various methods of treatment of pyometra in bitches. For this purpose, the study was conducted in a private clinic and veterinary hospital in Baghdad. The study included seventeen different breeds bitches. The ages of bitches range from 12-132 months with an average of 38 months. Bitches are diagnosed with pyometra (open and closed) depending on clinical signs, ultrasound, and complete blood count (CBC). Bitches were allocated into four groups...

Makmun1, Imam Mujahidin Fahmid2, Muhammad Saleh S. Ali2*, Muhammad Yamin Saud2, Rahmadanih3

...cted using a qualitative method; data was collected through interviews, observation, documentation, and focused group discussions. The method of selecting informants uses purposive sampling of farmers and the Animal Husbandry Department of Blitar Regency. The study’s findings indicated that actors involved in the laying hen farming business were small farmers, medium farmers, large farmers, farmer groups, poultry shops...

Nurhashunatil Mar’ah1, Safika Safika2*, Agustin Indrawati2

...ms using the Kirby-Bauer method. The Polymerase Chain Reaction (PCR) was used to detect the resistance genes and virulence factors of K. pneumoniae. Samples isolated from water sources showed a resistance profile to cefotaxime (100%), ceftazidime, ampicillin, and amoxicillin (50%). Samples isolated from udder rinses water were resistant to amoxicillin, cefotaxime, ampicillin (100%), and ceftazidime (60%). Samples obtained from milker hand swabs results were re...

Nurlan Akhmetsadykov1*, Tanatar Kydyrov2, Moldir Akhmetzhanova1, Gulnazi Akhmetova3, Maxat Berdikulov4 

...w serological diagnostic methods is highly relevant. The primary objective of this study was to produce a recombinant GM6 antigen derived from T. evansi and investigate its immunological properties for the detection and diagnosis of trypanosomosis. To create a recombinant antigen, bioinformatic analysis of the primary structure of T. evansi GM6 antigen from several regions was carried out. The protein sequence was reverse translated to the nucleotide sequence,...

Muhammad Talha Sajjad1, Hamid Akbar1*, Muhammad Arif Khan1, Muhammad Hassan Mushtaq3, Shehla Gul Bokhari1, Muhammad Abid Hayat4 and Ghulam Mustafa2

... is a safe and effective method. The color Doppler ultrasonography can be used to assess neovascularization during wound healing.

...

Kouengoua Kouengoua Armelle Prudence1*, Yessinou Roland2, Nankam Chimi Roland1, Fotsac Dzousse Muller1, Kouam Alain1, Djuikwo Felicite1, Facho Balaam1, Awah Ndukum Julius3, Farougou Souaibou2 

...ce, host, causal agents, methods of diagnostics, risk factors. After several screenings, six articles were considered for this systematic review. East and Southern Africa countries carry on most researches on bovine tuberculosis in wildlife. Available studies assessed the status of wild mammal, carnivore. Further researches to improve the knowledge on the status of bovine tuberculosis in wildlife in Africa are still needed in order to allow proper disease moni...

Vladimir Vasilievich Shimalov  

...als were examined by the method of complete helminthological dissections. The number of common shrews on drainage channel banks was 3.18 individuals per 100 trap-days. Infection with helminths was 98.4 %. 28 species of helminths were found (8 Trematoda, 10 Cestoda and 10 Nematoda). Cestodes Monocercus arionis (Siebold, 1850) and Neoskrjabinolepis singularis (Cholodkowsky, 1912) dominated the infection. The trematode Prosolecithus danubica Tkach et Bray, 1995 f...

Shakir Ullah1* and Lubna Shakir

...ey provide an analytical method for determining the potency and effect of these phytochemicals on living plants, cells, or tissues.

...

Skeikh Mustafizur Rahman1*, Mst. Shirin Sharmin Khan1, Yousef Ahmed Alkhamis2,3, Roshmon Thomas Mathew2, Md. Moshiur Rahman1, Mohammed Monirul Islam4, Md. Asadujjaman5 and Md. Golam Sarower1

...56%) between species and methods of cooking varied considerably. The highest amounts of protein (19.18 to 33.55%) were found in the fried fish, while the raw and boiled fish contained almost similar amount (protein, 14.00 to 19.57%). Fat content differed between species ranged from 0.38 to 4% in raw fish, 0.78 to 3.43% in boiled fish, and 15.72 to 33.78% in fried fish. The ash content also differed among raw (1.05 to 3.76%), boiled (0.51 to 3.00%) and fried (1...

Seyed Ahmed Reza Hashemi1, Teymour Aminrad1, Asadullah Ali Muhammad2*, Mastooreh Doustdar3 and Parastoo Mohebi Derakhsh3

...aximum sustainable yield method for this fishery in Iran.

...

Ahokpossi AGAC1,2*, Salifou CFA2, Youssao Abdou Karim I2, Ameyapoh Y3 

...s. Regardless of farming methods, the meat of Goliath chickens was accepted and appreciated by the panelists.  

...

Tinda Afriani1*, Jaswandi1, Yurnalis1, Putri Oktavially1, I Made Merdana2 

...FH cattle were used. The method employed involved a molecular analysis of growth hormone in the exon 5 gene using Polymerase Chain Reaction (PCR), alongside a qualitative analysis of the characteristics exhibited by the FH and Pesisir cattle crosses. The identification results regarding the diversity of the GH gene found 14 point mutations, including six transversions, namely, C>G 1343 bp, T>G 1376 bp, C>A 1438 bp, G>C 1570 bp, A>C 1720 bp, and ...
Syed Roohullah Jan1, Kareem Akhtar2*, Uroosa1, Muhammad Zeeshan Zahir3, Abdul Shakoor4
 
...erated using traditional methods undergo oxidation and contamination problems. Cold spray is recently used to obtain pure coatings without oxidation. In this work coatings of Ag-Sn-Cu powder are achieved on the Aluminum substrate and human teeth, using the high-pressure cold spray technique. The powder when mixed with Mercury is called amalgam and is commonly used in dental applications for cavity filling. However, Mercury is health hazardous. In the present s...

Wisnu Jaka Dewa1,2, Ekowati Handharyani3*, Sri Purwaningsih4, Silmi Mariya5 

...ing the ΔΔCt method. The result showed that bax gene expression increases 4.36, 4.77 and 4.63 times in 125, 62.5 and 31.25 ppm concentration, respectively. The expression of bcl-2 gene was decreased after treatment of red eye snail ethanol extract by 0.17, 0.16 and 0.14 in 125, 62.5 and 31.25 ppm extract concentration, respectively. Relatively, the bax:bcl-2 ratio was also increase. Red eye sea snail extract has a potential as alternative therapy o...

Budianto Panjaitan1, Husnurrizal Husnurrizal2, V.Y. Edward3, D.A. Nishcaya3, Teuku Armansyah4, Tongku Nizwan Siregar2, Mulyadi Adam5, Arman Sayuti1, Amalia Sutriana4, Hafizuddin Hafizuddin2*

...unosorbent assay (ELISA) method. The average spermatozoa volume (mL); concentration (106 cells/mL); and testosterone concentration (ng/mL) in P1 vs. P2, were 4.82±1.93 vs. 4.92±2.45; 889.0±250.50 vs. 721.0±246.25 (P<0.05); and 8.352.34 vs. 9.60±2.24 (P>0.05), respectively. It is concluded that the PGF2α injection 1 hour prior to the collection was able to increase the spermatozoa concentration compared to the 2 ho...

Salema Lafta Hassan1*, Taghred Jabbar Humadai1, Sabrin Ibraheem Mohsin

...unosorbent assay (ELISA) method. G1 revealed a significant decrease (P≤0.05) in the first group when compared to G2, G3, and G4. Histopathological examination of the testes revealed testicular degeneration, together with the absence of spermatids, no epididymal sperm in the epididymal tubules. In conclusion, Folcord has a severe toxic effect on mice, as evidenced by histological and immunological changes in the male reproductive mice, according to the resul...
Rahmat Ullah Khan*1, Karim Gabol1, Abbas Khan1, Asif Sadam2, Waheed Ali Panhwar3, Hamid Ullah5, Muhsin Ali4, Gul Bacha Khan4 and Habib Ul Hassan1
...by using the point count method in three main selected habitat types. A total of 112 bird species belonging to 15 orders, 40 families, and 71 genera occurring in the study area. Among the three habitat types, the more diverse and abundant habitat of birds was Creek (51%) followed by scrubland (27%) and Bhambore fort (21%) with Shannon diversity index (3.701). The more diverse and abundant bird species were the winter visitors followed by resident species. The ...

Siqiang Li1,2, Tiantian Wang1, Peng Sun1, Airong Gao1, Xin Gong1, Yuanhong Xu2, Baogen Wang2, Jun Wu1* and Bo Liu1*

...tional molecular cloning methods, and expressed in a 5-liter fermentation tank. The target protein was purified in three-step purification and identified by peptide mass of fingerprint. The enzymatic activity and optimal reaction conditions of MDS I were detected using DNA sequencer-assisted fluorophore-assisted carbohydrate electrophoresis. We obtained MDS I with a purity exceeding 90% in gram scales, which was capable of digesting α-1,2 linked mannose ...

Heni Indrijani*, Asep Anang, Muhammad Farhan Fadilah, Nena Hilmia, Maya Fitriani

...e same age. The research methodology used to measure the heterosis effect involved comparing the average parent with the average crossbreeding in terms of egg weight, DOC weight, fertility, and hatchability parameters. The results indicated that the crossbreeding between Sentul Debu and Sentul Kulawu chicken lines had a negative heterosis effect value for egg weight, DOC weight, and fertility, but a positive heterosis effect for hatchability.
 ...

Khaeruddin1, Gatot Ciptadi2, Muhammad Yusuf3, Iswati4, Setya Budhi Udrayana4, Sri Wahjuningsih2*

... using abdominal massage methods and divided into 4 tubes filled with diluent. For the analysis, the diluent selected was egg yolk Ringer’s lactate added with BHT at concentrations of 1, 2, and 3 mM, respectively as well as a control treatment without BHT. Liquid semen was stored for 72 hours at 5°C, with spermatozoa quality monitored every 24 hours in 10 replicated experiments. The results showed that sperm motility, viability, and plasma membrane i...
Yongtao Xu1, Dandan Wang1, Xiaolong Hu1, Minling Li1, Ming Tang1, Wuhua Liu2, Jianwen Zhan2 and Weiwei Zhang1*
...ed with traditional diet methods. This study assembled basic summer diet data and provided an important accumulation for the protection and monitoring of Taohongling sika deers, which also provides reference for the establishment of local database in the future.

...

Muhammad Tahir Latif1*, Muzzammil Hussain1, Ali Zohaib2 and Ishtiaq Hassan3

...nal tillage/broadcasting method (CT). As the SS has not been adopted on large scale to find out its adopters easily; therefore, twenty innovative farmers were included in sampling frame with non-probability sampling procedure for field survey as well as six field experiments were conducted in Gujranwala zone, Pakistan to evaluate four sowing techniques, i.e., CT, happy seeder, ridge sowing and SS with Randomized Complete Block Design. As compared to CT the SS ...

Muhammad Usman1*, Muhammad Uzair Khalid2, Muhammad Hasnain3*, Muhammad Tauseef4, Ali Raza4, Muhammad Akram4, Muhammad Shahid4, Abrar Ahmad4, Muhammad Shoaib Ismail1, Rabia Afzal2, Atta-Ulla2 and Muhammad Hussnain Babar3

...osmo-priming is the best method to increase the plant height (94.74cm), tillers (311.49 m-2), spike length (10.18cm), number of grains per spike (43.24), grain yield (4244.8kg ha-1), straw yield (8330.5kg ha-1) and Harvest Index (33.81%) especially in late-sown wheat. It should help to reduce the time to germinate and increases metabolic actions in plants. Healthy seedling germination will increase the yield of the crop. Moreover, it was concluded that osmo-pr...

Makruf Arif1*, Claude Mona Airin1, Dwi Sunu Datrianto2, Vincentia Trisna Yoelinda1, Sarmin1, Yuda Heru Fibrianto1, Pudji Astuti1

...lysis through the T-test method. The results indicated an increment of testosterone and corticosterone hormones among experienced Stone Magpie birds after the contest; however, these changes did not reach statistical significance. The mean values, both before and after the contests, of testosterone levels in the amateur group did not show a significant difference. However, corticosterone levels in this group showed significant differences (P<0.05), with pos...

Pramudya Andiana*, Moch. Geerhan Miraja Syahdan, Khothibul Umam Al Awwaly, Abdul Manab

... laboratory experimental method using a completely randomized design (CRD) was used in this research. In this study, chicken head protein was hydrolyzed using different combinations of bromelain (b) and papain (p), namely CHP (without hydrolysis); CHP1 (0.25% b and 0.75% p); CHP2 (0.5% b and 0.5% p); and CHP3 (0.75% b and 0.25% p). The data were analyzed using one-way analysis of variance followed by Duncan’s multiple range test. The results showed that ...

Noor A. Namaa*, Hayder A.N. Al-Zamely

...roved to be an effective method for identifying and characterizing specific proteins. Additionally, BCA demonstrated a highly sensitive and straightforward immunoassay for detecting elevated complete testicular protein. However, isolating the active components in Ginseng and creating unique NPs based on one of these components can result in the development of novel medicines with distinctive properties. Additionally, there is a clear need for greater research ...

Wael Halawa1, Raed M. Al-Atiyat2*, Jamal Abo Omar1, Faisal S. Rashaydeh3, Fath Ullah4, Shakirullah5 and Berkant Ismail Yildiz6

...ct of different suckling methods on newborn Assaf lambs growth performance and mortality. A total of 97 lambs were used in the study, with lambing periods starting from October 2020 to January 2021. Ewes’ parities were first, second, and third. After lambing, lambs were randomly distributed according to the suckling method into three groups: natural suckling (G1) (39 lambs), lambs fed completely with milk replacer (G2)...

Jiaojiao Wang, Wei Liu, Qingqian Lin and Jianhua Hou*

...a line transect sampling method at the eastern airport of Hebei Province from June to August, 2019. In total, 36 species belonging to 11 orders and 22 families of birds were recorded. Disturbances such as noise from aircraft flights affected the number of species and the individual density and diversity of birds. However, different areas were affected to different extents, and three areas on the same side were the most affected. Additionally, we found that the...

Phoebe Lyndia Tolentino Llantada1,2*, Midori Umekawa2, Shuichi Karita2

...l electrophoresis (DGGE) method to profile the bacterial community and PCR amplification using species-specific primer sets for 16S rDNA fragments to detect major fibrolytic and non-fibrolytic bacteria. Results from the PCR-DGGE analysis showed differences in bacterial diversity, density, and banding patterns along the gut of buffaloes. Higher bacterial diversity, density, and banding patterns were observed in the foregut and hindgut as compared with the midgu...

Frennada Lu’fatul Azizah1, Osfar Sjofjan2*, Eko Widodo2

...tiated using the Soxhlet method. Subsequently, 160 broiler chickens were utilized in the research. An experimental design in the form of a completely randomized design with five treatments and four replications was employed, involving eight broiler chickens per treatment per replication. The feed treatments were formulated as follows: T0 (basal feed), T1 (basal feed + 0.5% bacitracin), T2 (basal feed + 0.5% BSF larval oil), T3 (basal feed + 0.5% BSF pre-pupal ...

Ruth Dameria Haloho1*, Jisril Palayukan1, Agus Setiadi2, Edy Rianto2, Nadlirotun Luthfi3

...This study used a survey method. The locations were selected by purposive random sampling for the highest population of buffalo. One hundred buffalo farmers were taken as respondents by a multistage random sampling method. The data were obtained by observation and direct interviews the farmers. The data was analysed descriptively and quantitatively. The study showed that there are two kinds of buffalo in West Sulawesi, the f...

Abdul Haseeb1, Yasir Iftikhar1, Muhammad Ahmad Zeshan1*, Safdar Ali2, Rana Binyamin3, Salman Ghuffar4 and Muhammad Usman Ghani5,6

...itehead and Hemming tray method was used for the isolation of T. semipenetrans which showed that the population was low in declining orchards as compared to the population densities found in healthy orchards. Kinnow was the most infested by nematode with mean population 1161 while sweet orange was the least infested with 595 nematodes, in all localities. Maximum nematode population density in healthy and declining orchards was recorded in Kot Momin that was 26...

M. Imran Kasana, Rashid Iqbal Khan*, Noor Ullah Khan, M. Noman, Shahid Ali, Saima Mumtaz, Shamaila Rasheed and M. Qamar-Uz-Zaman

... is the most appropriate method for producing high-quality, true to type plants. The current study was planned to evaluate four different grafting techniques (Cleft, Tongue, Patch and T-budding) in ten different cultivars of avocado. The outcomes suggested that Cleft grafting was most suitable one among all other evaluated techniques. The highest survival rate (27.40%) was recorded in cleft grafting. Moreover, vegetative characters, i.e., no. of leaves and lea...

Diding Latipudin*, Chitra Kumalasari, Lovita Adriani

...nation with the oven-dry method. Dried probiotics can protect kidney cells from stress and free radicals in late-phase laying hens, which can be demonstrated by normal levels of creatinine, uric acid, and urea, thereby increasing productivity. The treatment consists T0: basal rations, T1: basal rations + 100% CM dried probiotic, T2: basal rations + 75% CM + 25% fermented MBM dried probiotic, T3: basal rations + 75% CM + 25% SM dried probiotic, and T4: basal ra...

Eluzia A. Barus*, Khansa N. Salsabilah, Achmad Firman, Linda Herlina

...nducted using the survey method at Camara Farm, Cigendel Village, Pamulihan District, Sumedang Regency, in October 2023. Data collection consisted of primary and secondary data obtained using the interview method. Based on the research results, Camara Farm’s average total production cost per period for a year was Rp. 2,342,573,664. The average revenue per production period was Rp. 2,429,151,070, so the average revenue ...

Munifah Siti Amira Yusuf1, Norsida Man2*, Nur Bahiah Mohamed Haris1, Ismi Arif Ismail3, Siaw Shin Yee1 and Tengku Halimatun Sa’adiah T Abu Bakar1,4 

...ing the PLS-SEM analysis method with the help of SMART-PLS software version 3.0. Findings revealed a positive association between all CIPP factors and Effectiveness. These findings showed that the DOA should prioritise process and product as the agriculture extension provider (stakeholders). This study suggests that policymakers should improve the implementation of urban agriculture programs in context, input, process, and product at a community level to impro...

Zhenglei Qiao*, Jie Xing and Fang Li

...e and maximum likelihood methods. We found that P. stoliczkana was closely related to Pethia ticto and Pethia padamya. These results enrich the mitochondrial genome database of Smiliogastrinae fish and provide reference materials for systematic classification of this group of fish.

...

Lijuan Liu1, Jinghang Chen2*, Min Wang3 and Wei Li4

... systemic administration methods such as oral or injection, inhaled medications are directly administered to the respiratory tract to exert a therapeutic impact, which has clear benefits in the treatment of respiratory illnesses. However, existing inhaled medications are difficult to fulfill clinical prescription demands, and the research and development of novel inhaled pharmaceuticals has significant hurdles due to a lack of comprehensive theoretical and pra...

Rogia SA Gomez*, Said H Mbaga 

...bility snowball sampling method. Information was collected on farmers, flocks, production costs and farm incomes. From this data, eight variables were selected and subjected to a principal component analysis (PCA), enabling three groups of farmers to be distinguished. The productivity of each group was assessed based on the economic information collected from the farmers. Overall, the results showed that the number of birds per flock was less than 1000 on farm...

Fazal Ur Rehman1*, Saeeda Fatehullah1, Ayaz Qadir1, Suleman Ahmad2, Siddique Usman2, Muhammad Asif2, Mukhtiar Ahmed1, Sunaina Rafi3, Muhammad Mohsin2, Ayesha Amanullah4, Faisal Shahzad2, Hasnain Raza2 

...icacy of open and closed method of anal sacculectomy for better outcomes in respect of post-operative complications in dogs. The study was performed on 16 adult dogs of either sex or breed with 1 to 3 years of age presented at University Outdoor Clinic which required anal sacculectomy. All the animals were categorized into two equal groups i.e. group-A and group-B. Group A underwent open anal sacculectomy and group 2 closed anal sacculectomy. To compare surgic...

Huda S.J1*, Nasr T.M1, Mohanad A. Al-Bayati2

...mosome via the Bangosome method. Anemic rabbit dosed with Nano hemosomal Cobalt-Ferrite 1/40of LD50% dose 75mg/kg Bw. Hematological parameters were analyzed in the blood samples that were collected from a rabbit. The outcomes of the hematological profile before and after hemorrhagic corrections were Hemorrhagic group RBC 5.96±0.11 x 106/mm³, WBC 6.35±1.04 x 103/mm³, PCV 33.02±6.28%, Hb 11.19 ± 0.88 g/dl and Platelet count ...

Ghaith Z. Hasan Al-Askari *, Eman H. Yousif Al-Taee

...sues of female rats. The methods included in the first experiment, the female rats were orally administered the following lethal doses: 0.5, 1, 1.5, 2, 2.5, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, and 16 mg/kg b.w. of vitamin D. Survival and mortality numbers were observed following 24 hours of intoxication. In the second experiment, the selected groups were divided according to the doses they received. In a cross-sectional study, 30 female rats were divi...

Taghreed Jabbar Humadai*, Bushra Ibrahim AL-Kaisei

...in male albino rats. The methods included sixty male albino rats at age 8-9 weeks and weighted 190-200 gm were divided into three groups: The first group: Consisted of 20 rats control were given distilled water and normal rat pellet, the second group: Consisted of 20 rats injected weekly 0.5µg /kg. b.w. by I/P route which represented 1/20 LD50 of tetrodotoxin, the third group: It also consisted of 20 rats injected weekly 1 µg /kg. b.w. by I/P route...

Ediset1*, Jaswandi2, Fuad Madarisa1, Amrizal Anas1, Rizqan2

...This study uses a survey method and a secondary data analysis approach. The population is 100 buffalo breeders who have adopted the AI. innovation. In comparison, the number of samples determined by the Proportional Sampling technique is 50 breeders from 5 sub-districts in Padang Pariaman Regency. Data analysis was carried out in a quantitative descriptive manner, namely the adoption rate of A.I. was measured by the percentage of adoption, and the success rate...

Hashim Hadi Al-Jebory1*, Mohammed Khalil Ibrahim Al-Saeedi2, Majeed Ajafar1, Nihad Abdul-Lateef Ali1

...ct of various levels and methods of adding melatonin to water and diets on the growth performance of broiler chickens.  One day old, 400 Ross 308 broiler chicks were randomly allocated into five random groups, with each group being quadruplicated and 20 birds allocated to each replication. Melatonin was supplemented in the G1 control group. G2 received 10 mg of melatonin per kg of diet, G3 received 20 mg of melatonin per kg of diet, G4 received 10 mg of m...

Nadya Indriyani1, Sri Murtini2*, Sri Darwati3, Cece Sumantri3

...le disease virus (AbNDV) methods using Hemaglutination Inhibition (HI) tests. Descriptive analysis was performed on production performance data and humoral immunity to estimate phenotypic correlation of the statistical method of Pearson correlation analysis. The results of IgY examination in men aged 27 weeks were 9.49 ± 1.37 mg/mL and IgY in women 9.36 ± 2.89 mg/mL. AbNDV ages 0 and 2 weeks at 1.34±0.95...

LAILA SHAHZAD*1, ARIFA TAHIR2 & FAIZA SHARIF3

... KPK Pakistan. Using mix method approach of interviews and questionnaire, data was collected from 200 randomly selected households. Temperature data of 30 years 1985 to 2014 showed that there is an overall decrease in mean minimum annual temperature by a factor of 0.0024 for each year whereas there is an overall increase in mean maximum annual temperature by a factor of 0.0412 for each year. The mean annual rainfall of thirty years was recorded as 1471.27 mm w...

SYED MUJTABA HAIDER1, KHIZAR HAYAT BHATTI1*, EJAZ HUSSAIN SIDDIQI1, SYED MAZHAR IRFAN1,`2, & ALLAH BAKHSH GULSHAN2

...ae). Agar well diffusion method was applied to assess the antibacterial activity of these extracts. Their antibacterial activities were associated with typical chemical like gentamicine (antibiotic) as positive control. This study revealed that S. pyogenes, S. aureus and N. gonorrhoeae had the maximum inhibition to different extracts of A. vera plant and selected plant extracts remained less effective against Shigella sonnei, E. coli (1) and E. coli (2). The d...

ZOHAIB SAEED1, SHAHID IQBAL*1, UMER YOUNAS1,2, MUHAMMAD PERVAIZ*3, SYED MOHSIN ALI NAQVI3 & RANA RASHAD MAHMOOD KHAN3

...ntalists. There are many methods for its disposal. The most suitable and economical method is its land application. Lagenaria siceraria L. plant was grown in various amendments of soil and sewage sludge to check the tolerance of the plant and to select the optimized mixing of sewage sludge with soil for this particular plant. Estimation of biochemical parameters like chlorophyll content, carotenoid content, and protein conte...

ZARYAB KHALID SIAL & FARAH KHAN

...zed Rapid Trizol reagent method was comparatively found best and reproducible as compared to the second method. The Sequencing and BLAST confirmed the presence of Pakistani PSTVd (Accession # MK.303578) in infected potato plants.

...

MUHAMMAD MANSHA1, MAIRA HAFEEZ1, QAMAR ALI1, ABRAR HUSSAIN1, MUHAMMAD TARIQ1 & ATIF YAQUB2

...ying agar well diffusion method. The microbes, Escherichia coli, Staphylococcus aureus and Candida albicans were cultured on agar plates. Wells in agar plates were loaded with 50 μl of 0.02 μg/ml and 0.04 μg/ml of hydrazone derivatives. Biological activity was examined by measuring the zone of growth inhibition. Compounds, 5, 6 and 11 exhibited none or negligible zone of inhibition against S. aureus. Compounds, 1, 4, 7, 8, 10 and 12, showed moderate z...

ASMA WAHEED QURESHI1* & SHUMAILA ALAM2

... assessed using standard methods. Overall parasitic contamination of 30.7% was observed. Highest contamination was detected in Mardan (36%) followed by Katlang (30%) while the lowest contamination was observed in Takhat bhai (26%). Hookworm (32.6%) was the most common contaminant found followed by Ascaris lumbricoides (21.7%) and Trichostrongylus spp. (10.87%) and the least detected parasite was Schistosoma (1.08%). Among vegetables, lettuce and mint showed hi...

MUHAMMAD JAVAID AFZAL*1, FARAH JAVAID2, MUHAMMAD WASEEM ASHRAF3, SHAHZADI TAYYABA4, MAMOONA ASHIQ5 & AYESHA AKHTAR5

...udy presents a simulated methodology on the basis of fuzzy logic for flow rate of drug delivery through a nanoneedle for drug delivery. The outflow rate is measured as 1.32 mL/min through MATLAB simulation. The measurements are verified by the Mamdani’s model and the previous research. The simulated results given in this study specifies that the fuzzy logic deliver an effective modeling presentation by showing good agreement with the parametric measureme...

SEHRISH MUSHTAQ1, MUHAMMAD SHAFIQ1, MUHAMMAD ASHFAQ*1 FAIZA KHAN1, SOHAIB AFZAAL1, UMMAD HUSSAIN1, MUBASSHIR HUSSAIN1, RASHID MUKHTAR BALAL2 & MUHAMMAD SALEEM HAIDER1

...sing the agar absorption method. This study showed the antifungal potential of Pantoea sp. (35.66%), Ensifer adhaerens (35.33%), Citrobacter diversus (33.03%), and Azotobacter nigricans (31.56%) to check the growth of pathogenic fungi compared to controls. Aureobacterium liquifaciens (27.66%), Acinetobacter sp. (25.66%), Bordetella pertussis (26.63%) also showed equal potential for inhibition. In contrast remaining isolates Enterobacter cloacae (19.33%), Azomo...

*SHAJAHAN BAIG1, MUSHTAQ AHMAD SALEEM1, MUHAMMAD AZMAT ULLAH KHAN1 ZEESHAN NADEEM2, SUFIAN AHMED2, MEMUNA GHAFOOR SHAHID3 & TANZEEM AKBAR CHEEMA3

...cs and protein profiling methods. These profiling methods are very useful but still studies on sensitivity, specificity and substantiation are needed. Furthermore, bioinformatics studies may be quite useful for the successful application of these profiling methods to analyze the safety of genetically modified (GM) foods. These bioinformatic methods emplo...

*ARIFA ZEREEN1, ALMAS JAHAN1,SHEIKH SAEED AHMAD2 & ZAHEER-UD-DIN KHAN3

...y Braun-Blanquet’s methodology was employed. In order to classify the vegetation data, ordination research tactics i.e., Two Way indicator species analysis (TWINSPAN) and Canonical Correspondence Analysis (CCA) were used. Vegetation data obtained from forty quadrats was compiled. Thirty eight plant species belonging to twenty one families were recorded. TWINSPAN bifurcated the flora of entire study zone into two main communities that had been again separ...

FOZIA HUMAYUN1, ZARRIEN AYUB2, MUHAMMAD SAMEE HAIDER3 & SYED ABID ALI1*

...essfully established the method of molecular identification of Siphonaria sp. The study also showed that Siphonaria sp. are nutritionally rich and thus can be consumed as food or feed as valuable marine resource to overcome the malnutrition problem in the developing countries.

...

SIDRA MOQADDES*¹, RABIA ZULFIQAR1, MAWRA GOHAR1 & HINA QAISER1

...r’s disc diffusion method. Samples (n=85) were analyzed to determine the bacterial strains involved in infectious cases and relationship with age, gender and social status of patients was determined. Results of the study revealed that 61% of samples were positive for Escherichia coli, other contributing pathogens were Staphylococcus spp. (24%), Klebsiella spp. (9%), Proteus spp.(4%) and others (2%). Out of 85 samples (march-june 2015), 48(56%) were from ...

ASMA ZAFAR*, MONIBA KHAWAR, MAHNOOR CHAUDHRY, SAYYEDA SANA BATOOL, AMJAD HUSSAIN** & MUSHTAQ AHMAD SALEEM

...d of medicine. Different methods are being used to eradicate cancer including chemotherapy, radiotherapy, immunotoxins, and drug therapy but these treatments cause various side effects to the cancer patients, as they not only target cancer cells but also cause harm to normal body cells. At present some live bacteria, bacterial products, and bacterial proteins are used to treat cancer. One of the amazing bacterial protein azurin can work as an anti-cancerous ag...

LAILA SHAHZAD*1, MUHAMMAD UMER HAYYAT1, NIDA SAJID1, FAIZA SHARIF1, ASMA MANSOOR1, MANAL SHAH1 & NABEEHA LODHI1

...f the community. Another methodology named Rapid Visual Screening (RVS) was used to determine the factors that contribute to infrastructure vulnerability to natural disasters. Factors assessed through this methodology are vertical and plan irregularity, soft storey, soft hazards, multiple storeys and building codes. Questionnaire results show that 70% people are educated and 90% are aware of high risk of area to natural disa...

UMER YOUNAS, SHAHID IQBAL* & JAMSHED AKBAR

...lts revealed that drying methods have no significant influence on recovery of antioxidants from sample matrix. However, methanolic extracts exhibited highest antioxidant potential that confirmed superiority of methanol as extraction media over the other solvents used. In addition, microwave-assisted extraction technique for the recovery of antioxidants from M. nigra leaves was optimized using response surface methodology. Fo...

1*AFIRA WAQAR, 2ADNAN RAUF, 3BUSHRA CHAUDHRY &4IKRAM-UL HAQ

...and the most appropriate methods for genetic analysis.

...

MUHAMMAD MANSHA*1, QAMAR ALI1, UROOJ FATIMA DILAWARI1, ABRAR HUSSAIN1 & MUHAMMAD TARIQ1

...i by agar well diffusion method. Chalcones analogues 1, 2, 3, 4, 5, 6, 7, and 8 were found to be inactive or insignificant against E. coli. Chalcone analogue 9 showed moderate activity against E. coli. Similarly, compounds, 1, 2, 3, 6, 7, 8 and 9 were found to be inactive or insignificant against S. aureus. Analogues 4 and 5 displayed moderate bioactivity against S. aureus. Similarly analogues 1, 2, 4, 7, 8 and 9 were found to be inactive or insignificant agai...

Sri Wahjuningsih1*, Ani Atul Arif1, Khaerudin2, Herlina Pratiwi3, Ardyah Ramadhina Irsanti Putri1

... collected using massage methods. This study uses a complete random design of factorial patterns with factor I being the equilibration time and factor II being the different thawing conditions. Fresh semen was evaluated macroscopically and microscopically. The sperm used had a motility of over 80%. The semen was diluted Lactate Ringer with 10% egg yolk and the addition of 7% DMSO cryoprotectant. Then the semen was equilibrated at different times and then froze...

 Muhammad Sultan1,4, Muhammad H. Mahmood2,4, Takahiko Miyazaki3,4, Shigeru Koyama3,4, Zahid M. Khan1

 

...easurements. Gravimetric method based magnetic suspension adsorption measurement unit was used for close-cycle adsorption kinetics measurements. However, the open-cycle adsorption kinetics was determined from the instantaneous measurement of air relative humidity and dry-bulb temperature in and out to the desiccant block while assuming the equilibrium between air and desiccant sides. Both experimental results were analyzed using linear driving force (LDF) mode...

Putri Okta Shafura1, Mardiati Zain2*, Elihasridas2, Bima Bagaskara1, Ummi Amanah1, Laras Sukma Sucitra1, Bella Veliana Utami1, Roni Pazla2, Erpomen2, Ezi Masdia Putri3, Rusmana Wijaya Setia Ningrat2, Riris Delima Purba3, Ruslan Abdul Gopar3, Putut Suryo Negoro3

...illey and Terry in-vitro methods were utilized to measure rumen characteristics, nutrient digestibility, protozoan population, microbial protein synthesis, and methane gas production. The results revealed a significant impact (P < 0.05) of these treatments on nutrient digestibility and rumen fermentation characteristics. The combination of 0.5% S. cerevisiae and 0.3% sulfur tended to increase nutrient digestibility, total VFA production, and microbial prote...

Firda Arlina1*, Mangku Mundana1, Linda Suhartati1, Agmelia Jasfaria2

...This study used a survey method, and sampling was carried out using purposive sampling. The parameters observed were qualitative and quantitative phenotypes. The data obtained were analyzed by descriptive statistical analysis. The results showed that the qualitative variety of the Randah Batu Kokok Balenggek chicken reared by the Kokok Balenggek chicken Farmers in Solok Regency, West Sumatra, based on the Biriang color of 48.33%, the yellow/white color of the ...

Bilal Saeed Khan1*, Muhammad Aneeb Shahzad1, Muhammad Irfan Ashraf2, Zahid Mahmood Sarwar3, Muhammad Farooq4 and Awias Rasool5

...oni Chant). The leaf dip method was used to conduct bioassay and this experiment was performed under Complete Randomized Design (CRD) with three replications. The results showed that N. tabacum extract was more toxic to aphids, two-spotted spider mites, ladybird beetles and predatory mites and caused 80.82, 81.49, 39.77 and 51.55% mortalities, respectively, after 96 hours. M. azedarach extract showed the second highest mortalities i.e., 73.85% in aphids, 70.54...

Waseem Abbas, Imtiaz Ahmed* and Imran Khan 

...performed using standard methods. In-vitro starch digestibility and total starch were determined by Megazyme starch assay kit. A nine-point hedonic scale assessed bread samples for consumer acceptance. The findings of proximate composition showed a significant increase in ash at 2 and 3 % incorporation. The fiber content also significantly increased at the incorporation ratio of 1, 2 and 3%. In CWB, fat concentration at 2 and 3% incorporation remained almost e...

Muhammad Wasif Gulzar*, Riffat Maqsood, Muhammad Zain, Muhammad Suleman, Tayyab Ur Rehman, Sana Asif, Abdul Wadood and Jawad Hussain

...n tissues. The preferred methods for routine diagnosis include PCR (Polymerase Chain Reaction), and Mouse Inoculation Test (MIT). In endemic locations, vaccinations with DNA, recombinant vaccines, and live, attenuated, or inactivated viruses can be administered. This study provides comprehensive information on immunization, treatment techniques, pathophysiology, epidemiology, transmission, and relevant preventive and control measures.

...

Yousef Abdal Jalil Fadladdin1, Ateeq Ullah2*, Raheela Nawaz3, Yagoob Garedaghi4, Abbas M.A. Al-Azab5 and Mashael Abdullah Aldamigh6

...rocessed in direct smear methods and examined under microscope first under low power objectives and then higher power Lense. Evidence of infection was noted by the presence of helminth eggs. Out of 200 samples 65% (n=130) were found to be infected with 2 species of soil transmitted helminths including 27% (n=54) A. lumbricoides and 35.5% (n=71) A. duodenale. Regarding ages, 11-12 years were highly infected. Females were observed to be infected more than male s...

Tanveer Jilani1*, Irfan Khan2, Asmat Salim2, Fareena Bilwani1, Bushra Moiz3 and Mohammad Perwaiz Iqbal1,4

...mination is minimal. The method of isolation and purification of CD34+-derived EPCs used in this study is simpler, user friendly and is more efficient as compared to other techniques. Human EPCs differentiated by this protocol can then be used to study normal and abnormal erythropoiesis.

...
Mouhamed Zakiou Kolawole Adissa Raimi1, Ghulam Hussain2, Nousheen Zafeer3, Faheem Ahmad1, Muhammad Irfan4, Anwar Ullah1, Imdad Kaleem1 and Asghar Shabbir1*
...und to be most effective method but this too have some significant side effects. Alternatively, natural products can potentially serve as an affordable and effective substitute for the treatment of MS with minimum or no side effects. Blackseeds (Nigella sativa) and honey possessing potent neuroprotective, antioxidant, and anti-inflammatory properties with no reported side effects can be a prospective candidate for an alternate remedial treatment of MS in anima...

Jin Jiao1, Jin Song1, Yuanyuan Cui1, Yilin Wang2, Yanling Li1, Jing Li1, Yan Su1, Xiaojing Du3, Yan Wang3, Guiping Sun3 and Minxiang Wei4*

...cross-sectional research method was used to recruit 190 patients who were at least one-week post-MI as the research participants. The Pittsburgh Sleep Quality Index, the Chinese version of the Multidimensional Fatigue Scale and the Hospital Anxiety and Depression Scale were used to analyse the relationship between fatigue and sleep disorders, anxiety and depression, assess the depression status and explore the relationship between myocardial infarction. In thi...

Shahzad Aslam1*, Amjad Rashid Kayani2, Muhammad Irfan Ashraf3, Muhammad Azhar Jameel2 and Kiran Sahar4

...tudy area. Food remnants method was used in combination with fecal analysis and visual observation for food composition. Both macroscopic and microscopic fecal analysis were carried out using 150 fecal pellets, collected from study area. The microhistological fecal analysis showed the presence of 29 dietary plant species as compared to 30 plant species observed in the field. No animal derived food component was noticed in the diet. The results revealed that fo...

Qurrat ul Ain Shafique1, Sana Batool2, Hanfa Ashfaq2, Asima Tayyab2, Roquyya Gul3 and Mahjabeen Saleem1*

...ved by stepwise dialysis method using urea gradient (8M-0M). Human IL-2 was recovered from 1 litre culture to a purity level of ~95%.  In vitro cytotoxic potential of IL-2 on HepG-2 and MCF-7 cell lines revealed that it possess sufficient cytotoxic potential and can inhibit the growth of these cell lines directly. Thus, refolded IL-2 had activity identical to that of authentic IL-2 and enhanced the anti-tumor activity of HepG-2 as compared to MCF-7 cells....

Dio Fico Felsidan Diatmono1, Seraphina Kumala1, Pradita Iustitia Sitaresmi2, Stefani Winda Paramita1, Megawati Andi1, Yustina Yuni Suranindyah3, Diah Tri Widayati1*

...y. The spectrophotometry method used for biochemical data using specifics enzymatic were used. Feed samples were analysed using proximate method to determine dry matter (DM), crude protein (CP), and total digestible nutrient (TDN) intake. The data results were analysed using Independent Sample T-Test and the correlation between blood metabolite profiles and nutrients was analysed using bivariate correlations by Pearson. The ...

Noor-ul-Ain1, Zulfiqar Ali1*, Saba Naz1, Kiran Saleem1,2, Syeda Rida Hasan1, Sana Arif1, Rida Ahmad1,3, Nida Naeem1, Zarmina Zainab1 and Aliza Batool1,3

...llected by scan sampling method of 70 h, documenting the cage area and behavioural ethogram. The observed behaviours were resting, eating, drinking, locomotion, aggression, urination, defecation, fighting, pacing, playing, foaming, cage exploration, and being out of view. Two different enrichments were installed for two pairs. The first pair was provided with a feeding enrichment consisting of a large wood log applied with small wooden branches on it, upon whi...

Aspen Abutalip*, Batyrbek Aitzhanov, Assiya Mussayeva, Vladislava Suchshikh, Natalya Yegorova

...es a constant search for methods aimed at its elimination. One such approach is the timely and rapid diagnosis of the disease, which will allow the timely implementation of preventive measures. The purpose of the study was to develop an express test to identify cases of animal morbidity for emphysematous carbuncle in the field. For this purpose, a serological method was used using the latex agglutination test (LAT). When pre...

Evy Rossi1*, Fajar Restuhadi1, Raswen Efendi1, Yusmarini1, Rahmayuni1, Emma Riftyan1, Bisma Panca Winata2, Yolanda Ashara2, Usman Pato1

...ack-sloping fermentation method could be used for up to seven and nine cycles for yogurt made from UHTM and FCM. The yogurts produced all meet probiotic drink standards with LAB content exceeding WHO standards, namely LAB content of more than 7 log CFU or 107CFU/ml.
 
Keywords | Starter, Lactic acid bacteria, Yogurt, Back-sloping fermentation
...

Advances in Animal and Veterinary Sciences

May

Vol. 12, Iss. 5, pp. 802-993

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