Effects of Vitamin C and Vitamin E on Cryopreservation of Guanzhong Donkey Semen
Effects of Vitamin C and Vitamin E on Cryopreservation of Guanzhong Donkey Semen
Xiaohui Yu, Shuai He, Liqiang Wang, Mengyang Kang, Yanjiao Zhu, Shuhui Wang and Xiuzhu Sun*
ABSTRACT
Sperm cryopreservation offers an effective method to protect Guanzhong donkey, a precious species. The objective of this study was to reveal the effect of different antioxidants on the semen cryopreservation in Guangzhong donkey by testing indices of sperm motility, mitochondrial activity, membrane integrity and arosome integrity. The results illustrated that compared with controls, above-mentioned indices of sperm were significantly improved under concentrations of Vitamin C (400 mg/L) and Vitamin E (600 mg/L) (P < 0.05). Moreover, physiological indices of donkey semen were significantly higher than the control groups (P < 0.05) when Vitamin C (200 mg/L) and Vitamin E (200 mg/L) were present simultaneitily in the medium. Overall, the results suggested both antioxidants (Vitamin C and Vitamin E) could increase frozen semen quality and the ability of antioxidant. Nevertheless, the effect on compatibility of the two antioxidants was better than single addition.
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October 2019
Vol. 51, Iss. 5, Pages 1599-1997
