Cellulase Production by Trichoderma viride in Submerged Fermentation using Response Surface Methodology
Cellulase Production by Trichoderma viride in Submerged Fermentation using Response Surface Methodology
Tehreem Zahra1, Muhammad Irfan1, Muhammad Nadeem2, Misbah Ghazanfar1, Qurratulain Ahmad3, Shaukat Ali4, Farzana Siddique5, Zarina Yasmeen6, Marcelo Franco7
ABSTRACT
The potential of Trichoderma viride for cellulase production using seed pods of silk cotton tree as substrate in submerged fermentation by response surface methodology has been investigated. Three variables like substrate concentration, peptone and KH2PO4 were optimized at three levels. The optimum carboxymethylcellulase (CMCase) activity and filter paper cellulase (FPase) activity was obtained after 96 hours of incubation with optimum conditions of media containing 5% substrate concentration, 0.05% peptone and KH2PO4 concentration of 0.5% with pH 5.0 at incubation temperature of 30ᵒC. The model proposed was found significant. The cellulase produced could be potentially used in industries especially for biofuel production.
Novelty Statement | This is first report on cellulase production using Bombyx cieba as substrate in submerged fermentation by Trichoderma viride.
To share on other social networks, click on any share button. What are these?
December 2020
Vol. 35, Iss. 2, pp. 167-268
