Effect of Intravenous Thrombolysis on the Early Treatment Time Window and Blood-Brain Barrier Marker Protein Expression
Effect of Intravenous Thrombolysis on the Early Treatment Time Window and Blood-Brain Barrier Marker Protein Expression
Zhongxi Zhang1, Ping Zou2*, Jingfang Zhang1, Yujuan Zeng1 and Yujie Tang1
ABSTRACT
The objective of this study was to explore the effects of intravenous thrombolysis on the early treatment time window and blood-brain barrier marker protein expression in rats with acute basilar artery occlusion. Sprague Dawley rats (n=180) were randomly divided into 6 groups, 30 in each group, which were sham operation group, model group, and intravenous thrombolysis for 0, 2, 4, and 6h groups. The rats were scored by Zea-Longa method. The effects of intravenous thrombolysis on brain injury and blood-brain barrier were detected by TTC staining. The expressions of ICAM-1 and MMP9 mRNA were detected by RT-PCR. The expression levels of ICAM-1 protein and MMP9 protein were detected by immunohistochemistry and Western blot. We found that compared with the model group, intravenous thrombolysis could significantly improve the behavioral disorder of rats, and significantly reduce the infarct size of rat brain tissue, and the expression levels of ICAM-1 protein and MMP9 protein. We conclude that intravenous thrombolysis could significantly improve the therapeutic effect of acute basilar artery occlusion in rats within 2-4 h, and the treatment effect was the best at 4h.
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