Characterization of the Complete Mitogenome of Mikrogeophagus altispinosus (Cichliformes: Cichlidae) and Phylogenetic Analysis of New World Cichlids
Characterization of the Complete Mitogenome of Mikrogeophagus altispinosus (Cichliformes: Cichlidae) and Phylogenetic Analysis of New World Cichlids
Ye-Ling Lao, Jin-Long Huang, Cheng-He Sun, Xiao-Ying Huang, Ting Wu and Qun Zhang*
ABSTRACT
Following rapid advances in complete mitochondrial genome determination, researchers have published the mitogenomes of many New World cichlids. However, few phylogenetic analyses of New World cichlids have been conducted. In this study, we determined the complete mitogenome of Mikrogeophagus altispinosus. After sequencing with the Illumina HiSeq 4000 platform, SPAdes v3.10.1 was used for assembly, and MITOS2 and MitoFish were used for annotation. We then successfully annotated the mitogenome of M. altispinosus, which has a total length of 16,767 bp. The gene composition and base preferences of M. altispinosus are similar to those of other New World cichlids. Based on the concatenated sequences of 13 protein-coding genes and two rRNAs from the mitogenomes of 37 New World cichlids and one African cichlid, we reconstructed phylogenetic trees using maximum likelihood and Bayesian methods and verified the classification of the target species M. altispinosus. According to our analysis, further studies should focus on the relationships between Amphilophus and Symphysodon. By determining the complete mitogenome of M. altispinosus, this study improves the phylogenetic resolution of New World cichlids. This new resource is highly important for the classification of New World cichlids and provides valuable data for subsequent studies on Cichlidae evolution.
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