Comparative Studies on Evaluation of Different Avian Influenza Inactivated Vaccines under Local Conditions in Egypt
Comparative Studies on Evaluation of Different Avian Influenza Inactivated Vaccines under Local Conditions in Egypt
Nermeen A. Marden1, Lamiaa M. Gaafar1, Hussein A. Hussein2
ABSTRACT
Background: Vaccination strategies have an important role in the limitation of viral infection and
spread to the surrounding environment. The evaluation methods of the AI vaccines are very important
for controlling the license of the bad vaccines. Comparative studies between the invitro and invivo
methods for 8 types of AI vaccines (5 batches for each) to evaluate their efficacies were done.
Methods: The invivo method, was done through the vaccination-challenge pathway of SPF chickens
to estimate the protection percent of each vaccine against both classical and variant HPAI viruses.
While, the invitro method was done by the direct detection of the viral content and viral identity using
the rRT-PCR technique from final product of these inactivated vaccines.
Results: The data of this study showed that sufficient HA antigen content and similar HA sequences
with the field HPAI challenge viruses are needed to produce a consistent and high protection percent,
but, they are not enough to assess the vaccine efficacy.
Conclusion: The invitro assessment of protection was not considered an alternative methods to the
direct assessment of vaccine efficacy (vaccination-challenge), but it was used as complementary steps
for vaccine evaluation protocol.
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July 2018
Vol. 4, Pages 1-71
