Oral Liposome Vaccines Containing Cyprinid herpesvirus 3 (CyHv3) and Aeromonas hydrophila Antigen for the Immunization of Common Carp Cyprinus carpio
Oral Liposome Vaccines Containing Cyprinid herpesvirus 3 (CyHv3) and Aeromonas hydrophila Antigen for the Immunization of Common Carp Cyprinus carpio
Sundus Oun Ali Al-Zaini1, Mohanad A. Al-Bayati3*, Khazaal Abbas Khazaal2, Salma Talib Salih1
ABSTRACT
This study aims to develop two oral vaccines for immunization against Koi Herpesvirus (KHV) and Aeromonas hydrophila antigen in common carp, Cyprinus carpio, using formalin-inactivated versions of both virus and bacteria (mean body weight of 108.2±1.2g). Vaccines that have been prepared and loaded into Nano liposome blank that have been formulated according to the thin film method (Bangham). The experimental was designed into four fish groups for vaccinination. The Formulated vaccines (viral and bacterial) was vaccinated once daily for fish along five days, dosed: 0.63g/fish/day and 1×109cfu/fish/day, respectively. The first two groups of fish were challenged by mingling them with KHV-infected fish at a ratio of two infected fish to ten vaccinated fish under simulated acclemtized normal condition. In contrast, fish from the second group were challenged with live Aeromonas hydrophila at a dose of (3×108cfu/fish and 3×107cfu/fish) for 22 days then had been vaccinated single dose. Preliminary findings reveal that fish groups treated with KHV liposomes pharmaceutic vaccine exhibit Relation present survival of 45% protection against KHV infection in bad quality water. Similarly, in fish groups raised in high-quality water, Relation present survival 62.5 % protection was test screening in fish groups. In contrast, fish groups treated with Aeromonas bacterium liposomes had Relation present survival values of 67.50% and 78.65%, respectively, against bacterial infection. This result enables us to initiate the subsequent phase of our research, wherein we employ tissue culture of fin cells from common carp fish to propagate the Koi herpes virus. The objective is to develop a vaccine with a dose containing the antigenic standard of 1×102.5 TCID50/dose, aiming to achieve a heightened level of immunity compared to the outcomes observed in the initial phase. Additionally, we aim to combine the two vaccine agents, namely the virus and bacterial components, into a single product. Conclusion the study successfully developed and tested two liposomal vaccines for common carp, one against Koi Herpesvirus (KHV) and another against Aeromonas hydrophila bacteria. The vaccines were prepared using formalin-inactivated virus and bacteria and encapsulated in liposomes formulated using the thin film method. KHV vaccine: 45% protection against KHV infection in fish raised in bad quality water. 62.5% protection in fish raised in high-quality water. Whereas Aeromonas hydrophila vaccine: 67.50% protection against bacterial infection, 78.65% protection in another group of fish.
Keywords | Aeromonas hydrophila, Common carp, Cyprinus carpio Liposome, Virosome
To share on other social networks, click on any share button. What are these?
July 2024
Vol. 12, Iss. 7, pp. 1206-1409
