Reconstruction and Subcellular Localization Analysis of Eriocheir sinensis Molting Protein-Protein Interaction Network
Reconstruction and Subcellular Localization Analysis of Eriocheir sinensis Molting Protein-Protein Interaction Network
Bin Wang1, 2, Qianji Ning1,*, Qian Wang2, Wei Peng2, Tong Hao2,*and Jinsheng Sun2,*
ABSTRACT
Molting abnormalities of Chinese Mitten Crab (Eriocheir sinensis) can seriously affect their growth, causing significant losses to the aquaculture industry. The current studies on the molting mechanism mainly focused on the independent analysis of single protein, rather than a protein-protein interaction. In this work, with the systematic point of view, the subcellular location of 830 unidentified proteins were annotated based on the previously reconstructed protein-protein interaction network (PIN) of E. sinensis using the bioinformatics analysis method, which accounts for 91.9% of all un-localized proteins in the network. Subsequently, the molting interaction network was extracted from the global PPI, which contains 35 proteins and 32 interactions. By analyzing the subcellular annotation of the molting interaction network, the subcellular localization of 12 proteins was obtained. Half of these proteins located on the cell membrane, indicating the close relationship between molting process and the changes of membrane. The results of this work provide a theoretical basis of the further elucidation of the molting mechanism for E. sinensis.
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October 2018
Vol. 50, Iss. 5, Pages 1601-1998
