Effects of Calcitonin on Porcine Intestinal Epithelial Cells Proliferation, Phosphorus Absorption, and NaPi-IIb Expression
Effects of Calcitonin on Porcine Intestinal Epithelial Cells Proliferation, Phosphorus Absorption, and NaPi-IIb Expression
Xiaopeng Tang1, Wen-qin Su1 and Re-jun Fang1,2,*
ABSTRACT
To investigate the effects of calcitonin (CT) on cell proliferation, NaPi-IIb expression, and the absorption of phosphorus in porcine intestinal epithelial cells (IPEC-1). The experiment consisted of 1 control treatment and 5 levels of CT treatments (1×10-12 to 1×10-8 mol/L), and each treatment has 6 replications. Cell proliferation was determined by the MTT method. Cell morphology and phosphorus concentration in the cell supernatant were measured after 24 h of CT treatment. The NaPi-IIb protein expression was determined by Western Blot, and the NaPi-IIb mRNA expression was determined by RT-PCR. The results showed that, compared with the control group, different levels of CT had no effect on cell proliferation, but it inhibited (P < 0.05) the absorption of phosphorus at CT concentration of 1×10-11, 1×10-10 mol/L and 1×10-9 mol/L. There was no effect of CT on NaPi-IIb mRNA and NaPi-IIb protein expression. The study suggested that CT can inhibit the absorption of phosphorus in porcine intestinal epithelial cells without affects NaPi-IIb expression.
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December 2019
Vol. 51, Iss. 6, Pages 1999-2399
