Standardization of Surgical Site Preparation with Different Formulations of Povidone-Iodine, Chlorhexidine-Gluconate and Chlorxylenol in Caprine Model: A Comparative Study
Standardization of Surgical Site Preparation with Different Formulations of Povidone-Iodine, Chlorhexidine-Gluconate and Chlorxylenol in Caprine Model: A Comparative Study
Md. Al-Amin Tan1, Mst. Antora Akter1, Md. Sabuj Rahman1, Marzia Rahman2 and Md. Mahmudul Alam1*
ABSTRACT
One of the major causes of surgical site infection (SSI) is an inappropriate surgical site preparation that leads to postoperative complications. Although preoperative skin preparation is a standard surgical practice to prevent SSI, the choice of skin disinfectants and methods of skin preparation is still aberrant in veterinary practice. The study was, thus, conducted to standardize pre-surgical skin preparation with various formulations of Chlorhexidine gluconate, Chlorxylenol, and Povidone-iodine (PI) in goat. Seven surgical fields were prepared for evaluating seven formulations of these antiseptics. The bacterial swabs collected at different stages of skin preparations were transferred in nutrient broth and cultured in Plate Count Agar for counting of Colony Forming Unit (CFU). Results revealed that Chlorxylenol was less efficacious than PI and Chlorhexidine gluconate when mean CFU was counted at different stages of surgical field preparation. Soap water scrubbing and ethanol spray followed by aqueous Chlorhexidine gluconate painting eliminated 100% bacterial load and kept the site aseptic for 60 min. On the other hand, alcoholic Chlorhexidine gluconate completely removed bacterial burden from the skin and maintained 90 min long aseptic condition of the operation site. Based on our findings, alcoholic Chlorhexidine gluconate was the best in producing an aseptic surgical field for a longer duration. Our findings recommend robust scrubbing of the surgical site with detergent or soap water followed by 70% ethanol or hexisol spray and then painting the site with antiseptic preferably with alcoholic Chlorhexidine gluconate solution to generate aseptic surgical site which is very basic to prevent SSI and postoperative complications.
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