This study aimed to identify polymorphisms of the (IGF-1) gene in Alope chickens and its relationship to the growth traits of Alope chickens. A total of 120 animals consisting of 52 cocks and 68 hens, were included in this study. Chickens were kept in individual cages to observe growth traits, including initial body weight, final body weight, daily body weight, body weight gain, feed consumption, and feed conversion ratio. Growth traits were analyzed using the general linear model method (GLM). To identify polymorphism using the PCR-Restriction Fragment Length Polymorphism (PCR-RFLP). Genotype frequency, allele frequency, and Hardy-Weinberg equilibrium were analyzed in this research. The research showed that three genotypes, AA, AB, and BB, were successfully visualized. The AB and BB genotype of the IGF-1 gene was significantly related (P<0.05) with final body weight, feed consumption, and feed conversion ratio in hens Alope chicken. So the IGF-1 gene has the potential to be used as a genetic marker for the initial selection process of Alope chickens.
Keywords | Alope chicken, Growth traits, IGF-1, PCR-RFLP, Polymorphisms