Evaluation of In Vitro Antibacterial Potential of Bacillus pseudomycoides Strain SB138
Evaluation of In Vitro Antibacterial Potential of Bacillus pseudomycoides Strain SB138
Rabiea Pervaiz, Shaista Bano*, Sarfraz Ali Tunio, Abdul Nabi Jatt and Aisha Amber Soomro
ABSTRACT
Bacillus species provide a natural source for the discovery of novel antibacterial substances. However, some of the Bacillus species still remained to be explored widely for their antagonism against harmful bacteria. One of them is Bacillus pseudomycoides which produces rhizoidal colonies on solid medium. The present study was aimed to explore antibacterial properties of B. pseudomycoides isolated from the rhizosphere soil of the indigenous Mangifera indica (mango) plant. Identification of the isolated strains was determined by sequencing their 16S rRNA genes. The isolates identified as B. pseudomycoides were screened for their antibacterial potential. In order to circumvent the trouble created from rhizoidal colony morphology of B. pseudomycoides, a colony mutant of a strain showing strong antibacterial activity was obtained by screening/selection method and designated as B. pseudomycoides strain SB138m. The sequence of 16S rRNA gene of both wild type and mutant strains of B. pseudomycoides was comparable. In vitro test for antagonism of B. pseudomycoides strain SB138m against indicator cells showed that the strain contained antibacterial potential. The present study shows inhibitory spectrum of B. pseudomycoides SB13m with conclusion that Bacillus species from a diverse environment and Proteus mirabilis from clinical environment were strongly inhibited.
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