Phylogenetic Relationship of Locally Isolated Paramecium Species Inferred from Histone H4 Genes
Phylogenetic Relationship of Locally Isolated Paramecium Species Inferred from Histone H4 Genes
Fareeda Tasneem1, Farah Rauf Shakoori1 and Abdul Rauf Shakoori2,*
ABSTRACT
The histone H4 gene is supposed to be highly conserved among all organisms with the exception of variations found in the ciliate species. Thus a fragment of histone H4 gene (160bp) was sequenced in ten locally isolated strains of Paramecium species including a standing-alone on the basis of 18SrDNA gene sequence strain FT8. In order to readdress the relationships of FT8 strain with other strains of Paramecium species, a molecular phylogenetic analysis was performed on the basis of H4 gene sequences. A phylogenetic tree was constructed using the aligned sequences of our study with 22 entries of closely related species from GenBank Data. Highly polymorphic sites were observed in FT8 strain sequence as compared to other species. Analysis of H4 gene sequences in our study showed they are closely related and behaved as a good substitute for phylogenetic analysis. Phylogenetic tree constructed by maximum likelihood method placed the FT8 strain very distinctly from other species, giving it a clearly separate position. Phylogeny results based on the H4 gene sequences corresponded to the results obtained by 18SrDNA sequences. Both markers proved the FT8 strain is a highly divergent species based on the phylogenetic relationships, opening new realms for the researchers of this field.
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October 2017
Vol. 49, Iss. 5, Pages 1547-1936
