Sehrish Kanwal1, Ali Saeed1*, Muhammad Munir2, Memoona Arshad1
Life Sciences International Journal Issue 7 Volume 1
...The virus was primarily detected with RT-PCR with P1/P2 and 1F/1R universal primer pairs directly from field samples including tissues, vesicle and secretion. The direct sequencing and subsequent analysis of amplified PCR products for VP1 gene indicated the circulation of serotype O of FMD in studied areas. Moreover, using serotype-specific primers (SA(F)/SA(R)) which target the VP1 gene, serotype O was confirmed in all representative samples. As high as 98% n...
Sehrish Kanwal, Ali Saeed, Muhammad Munir, Memoona Arshad
British Journal of Virology
...The virus was primarily detected with RT-PCR with P1/P2 and 1F/1R universal primer pairs directly from field samples including tissues, vesicle and secretion. The direct sequencing and subsequent analysis of amplified PCR products for VP1 gene indicated the circulation of serotype O of FMD in studied areas. Moreover, using serotype-specific primers (SA(F)/SA(R)) which target the VP1 gene, serotype O was confirmed in all representative samples. As high as 98% n...
Sehrish Kanwal1, Ali Saeed1*, Muhammad Munir2, Memoona Arshad1
British Journal of Virology
...The virus was primarily detected with RT-PCR with P1/P2 and 1F/1R universal primer pairs directly from field samples including tissues, vesicle and secretion. The direct sequencing and subsequent analysis of amplified PCR products for VP1 gene indicated the circulation of serotype O of FMD in studied areas. Moreover, using serotype-specific primers (SA(F)/SA(R)) which target the VP1 gene, serotype O was confirmed in all representative samples. As high as 98% n...
Lei Chen, Ling Zhu, Zhi-Wen Xu, Wan-Zhu Guo
Molecular Genetics of Aichivirus C (Porcine Kobuvirus) in China
... China. Due to the high detection rate in the severe diarrhea in China, Aichivirus C is thought to be a potential pathogeny of pig diarrhea. The review represented the discovery of Aichivirus C in China, and made a brief summary about molecular and epidemiology characterizations of Chinese Aichivirus C strains. Mutiple Aichivirus C strains and Aichivirus C variants are circulating in China. Recombination events were also observed in Chinese Aichivirus C strain...
Chun-Yi Lin1, Meng-Ling Wu2, Tang-Long Shen1, Hsin-Hung Yeh3, Ting-Hsuan Hung1*
...ime RT-PCR were used to detect the current status of the two viroids showing HSVd was more prevalent than CEVd and simultaneous infections were common in field. Uneven distributions of both viroids also occurred in different tissues of citrus. Two multiplex molecular detection methods provide the understanding of the genetic diversities among viroid isolates and quantify viroids in citrus host. Our field survey can help clar...
Hai-Bo Wang, Qiu-Hua Mo, Ze Yang*
...que was applied for the detection of diarrhoea related viruses. This mini review overviewed the basic principle of RT-NASBA and discussed the significance of these new findings....
Shahid Iqbal Awan1*, Syed Dilnawaz Ahmad2, Muhammad Amjad Ali3, Muhammad Shahzad Ahmed1, Aurangzeb Rao1
...er genetic distance was detected among the members of cluster 6 and cluster 8 and cluster 7 and 8. Members of these two clusters can be utilised in transgressive breeding....
Oladipo Elijah Kolawole*, Oloke Julius Kola, Awoyelu Elukunbi Hilda, Olasunmibo Oluwatobi Gabriel, Ogunyale Tolulope Temitope
E-mail | koladipo2k3@ yahoo.co.uk
Rodolfo Villagra-Blanco1*, Gaby Dolz1, Danilo Montero-Caballero2, Juan Jose Romero-Zuniga2
...ELISA). Antibodies were detected in 290 sheep from fourteen different flocks, distributed in all analyzed regions (Central, Chorotega, Atlantic Huetar, North Huetar, and Central Pacific) determining regional seropositivity between 63.5% and 100.0%, as well as an overall prevalence of 80.8 %. The within flock seropositivity percentages ranged between 0% and 100.0%. Flocks with the highest seropositivity were found in low altitude regions close to the coast. Ris...
Shumaila Manzoor1, Afshan Ahmed1 and Muhammad Abubakar2*
...) structural antibodies detection in terms of their sensitivity and specificity. These methods were performed by using set of sera collected from cattle with foot-and-mouth disease vaccination. The pattern of antibody titers in animals of different age groups was observed as less than 1 year, 1-2 years and more than 2 years for both ELISA and SNT. The peak immune response measured by SNT was 2.8log10 whereas ELISA d
Tomas Jinnerot1*, Karin Malm1, Erik Eriksson1 and Jonas Johansson Wensman2
E-mail | Tomas.Jinnerot@sva.se
... real-time PCR for detection of B. bronchiseptica in samples from dogs with respiratory signs. A genome comparison program was used to select a suitable target DNA sequence. A PCR targeting the bfrZ gene was developed which showed no cross-reactivity in silico or when tested with a panel of bacterial isolates. The limit of detection was determined to 4x103 bacteria per mL...
Claudia Kohl*, Andreas Nitsche, Andreas Kurth
Email: kohlc@rki.de
...eic acids, to allow for detection via metagenomic NGS. This minireview is revising our developed TUViD-VM protocol and selected other approaches regarding their suitability in metagenomics. We provide an overview on the difficulties, challenges and opportunities that developed alongside metagenomic virus discovery. The field of metagenomics from clinical specimens promises the identification of novel, yet unknown, infectious diseases and etiologies....
Rongfei Lu1, 2, Zhiyang Liu1, Peng Fang1, Guangyi Chen1, Feng Sun1, Yongjian Fan1, Yijun Zhou1, Tong Zhou1*
... current field of virus detection and disease dynamics. The validity and the reliability of generated data can be enhanced significantly appropriate measures. As a standard for the relative expression of target genes, the selection of reference genes is crucial. This review describes the history of the RT-qPCR technology, emphasizes the importance of reference genes and enumerates several algorithms to screen reference genes to normalize the RT-qPCR data...
Jonas Johansson Wensman1*a, Karl-Johan Leuchowius2,3 a, Jiting Yan1, Anna-Lena Berg4, Liv Bode5, Hanns Ludwig5, Sandor Belak6, Ulf Landegren2, Ola Soderberg2, Mikael Berg6
...situ PLA was applied to detect BDV P in brain tissues of infected animals. Finally, protein-protein interactions were visualized in both C6BV and brain tissues of experimentally as well as naturally infected animals (rat and horse, respectively). BDV proteins and their interactions with host proteins could be shown in cell cultures (HMGB1, Cdc2) and in brain tissues of rat (HMGB1, Cdc2) and horse (Cdc2 only) infected with BDV. In this study, we have for the r...
Yongfeng Li, Mo Zhou, Xiao Wang, Libao Xie, Hua-Ji Qiu*
...lent option to directly detect viral replication without the use of secondary labeling. This mini-review discusses the applications of reporter CSFV in the study of CSFV biology, including efficiently screening the antivirals against CSFV and viral receptors, conveniently tracking the viral proteins in live cells as well as improving diagnostic methods and vaccines for classical swine fever....
John G. Bruno1*, Chien-Chung Chao2,3, Zhiwen Zhang3, Wei-Mei Ching2,3, Taylor Phillips1, Allison Edge1, Jeffery C. Sivils1
...e ratio of > 4.0 for detection of ~ 1,000, R. typhi cells. Titration experiments conducted in buffer revealed a limit of detection between 100 and 1,000 R. typhi cells per ml. The Rt-18R aptamer detected only one band at ~ 84 kD in R. typhi lysates on aptamer Western blots. While the homogeneous Rt-18R fluorescent sandwich aptamer-magnetic bead assay did not cross-r...
Haq Aman Ullah1*, Aneela Zameer Durrani2, Muhammad Ijaz2 and Aqeel Javeed3
...amples Aflatoxin B1 was detected in 33 feed samples, with a percent contamination rate of 83%. The level of aflatoxin B1 ranged from 0 to 225.736 ppb in different feed samples. The maximum level of aflatoxin B1 was detected in cotton seed cake (mean level 137.059 ± 22.293 ppb) followed by wanda, homemade concentrate mixture and wheat bran.. Amongst the positive concentrate feed samples, 28 samples (85% of the positive...
Moustafa Kardjadj
... surveillance system to detect and respond effectively to exotic infections. Emergence and re-emergence of FMD virus genotypes/lineages has been detected necessitating frequent replacement of the vaccine strains. The present review summarizes the knowledge generated in epidemiology, diagnosis and surveillance of the disease in the Maghreb countries....
Moustafa Kardjadj1, Pam Dachung Luka2
... However, the increased detection of the virus in the light of rinderpest eradication and the lack of awareness of the disease in areas where the virus has been detected for the first time in recent years given the educational status of farmers contributed to this emergence....
Ahmed Zein Mahmoud1, Muaz Abdellatif 2, 3*, Luai Shazali1
...subjected to antibodies detection using c-ELISA. Out of examined animals, 83 (62.9%) goats and 70 (33.2%) sheep were detected positive against PPRV antibodies, whereas camels appeared to be seronegative. Based upon the seasonal variations in the antibodies detection, environment appeared to be a significant factor on the level of antibodies in the tested small ruminants population. Taken t...
Mohammad Mushfiqur Rahman1, Rokshana Parvin1, Ataur Rahman Bhuiyan1, Mohammad Giasuddin2, Shah Md. Ziqrul Haq Chowdhury3, Mohammad Rafiqul Islam1, Emdadul Haque Chowdhury1*
...study was undertaken to detect and characterize the PPR virus (PPRV) circulating in Bangladesh. Twelve local isolates of Bangladeshi PPRV were successfully detected from post mortem samples (lymph node, spleen and trachea) of goats and a sheep during the year 2008-2012. The partial N gene of ten isolates and partial F gene of eight isolates were amplified by RT-PCR, and were sequenced. The phylogenetic analysis revealed that...
Neha Singh and Anjana Pandey
...pes of cancerous cells, detect live pathogenic organisms, and to discover novel disease biomarkers to facilitate early detection of the disease and for therapeutic purposes....
John G. Bruno and Jeffery C. Sivils
...ular weight matching of detected electrophoretic bands across a spectrum of related and unrelated bacteria....
Koksal Karadas1, Muhammad Tariq2, Mohammad Masood Tariq3* and Ecevit Eyduran4
...e Automatic Interaction Detector), Exhaustive CHAID, and a ANN type MLP (Multilayer Perceptron) in the prediction of lactation milk yield in sheep was measured comparatively by model assessment criteria, such as R2, R2ADJUSTED, SDRATIO, CV(%), RMSE, RAE, MAPE, MAD and Pearson correlation coefficient (r) between actual and predicted milk yield values, respectively. Minimum farm numbers for parent and child nodes were ...
Zisha Liu1, Na Song1, Takashi Yanagimoto2, Zhiqiang Han3, Bonian Shui3 and Tianxiang Gao3*
...Three species were only detected termination-associated sequence domain (TAS) and conserved sequence blocks domain (CSB-1, CSB-2 and CSB-3). The Bayesian and ML tree topologies of 19 Gobiidae represented two groups: one large group consisted of Amblyopinae, Gobionellinae, Oxudercinae and Sicydiinae, and the other was the monophyletic Gobiinae. Phylogenetic analysis also demonstrated clade Amblyopinae included all three species and that
Atul Sharma, Aruna Chandra Singh, Gautam Bacher, Sunil Bhand
...ensing applications for detection of specific target molecules. In the present review, we concentrate on the recent advances in the development of aptasensors for antibiotics residue analysis based on electrochemical signal generation. Aptamers possesses the strong potential as receptors for the development of biosensors for antibiotics detection; therefore, a specially designed aptamer specific to an antibiotic may be suita...
Adedeji A.J, Sati N.M, Pewan S.B, Ogbu K.I, Adole J.A, Lazarus D.D, Ijiwo S.J, Okpanachi A, Nwagbo I.O, Joannis, T.M and Abdu P.A
...(PCR). IBDV antigen was detected by AGID while CAV by PCR. This confirms the diagnosis of concurrent infection of CIA and IBD in the affected flock. This is the first case report of a concurrent and natural field outbreak of CIA and IBD in a commercial poultry farm in Nigeria....
John Gachohi, Simon Karanja, Salome Wanyoike, Nduhiu Gitahi, Salome Bukachi, Kenneth Ngure
...water samples. Antibody detection in animal and human serum samples will utilize Microscopic Agglutination Test (MAT). Study outcomes will include (i) geo-referencing of adverse environmental attributes that support Leptospira growth, (ii) animal and human seasonal public health burden of Leptospirosis, (iii) seasonal variation in the distribution of Leptospira determinants in a slum setting, and (iv) a simulation model based on an ecological metapopulation fr...
Pan-pan Guo1, Wei Liu2, Yan Li2, Rui-yu Ma2, Wang Zaigui1*, Kai Zhan2*, Jun-ying Li2 and Sheng-nan Liu2
...0831C and G62976A) were detected and located in exon 42 and intron 54 in HTT gene, respectively. One of them (g. 50831 G>C) was a missense mutation. Genotype and allele frequency analysis showed that SNP G62976A and SNP G50831C of the HTT gene in chicken had some effects on reproduction traits, and the genotype GG was the advantageous genotype. Additionally, four HTT haplotypes (H1: GG; H2: GC; H3: AG; H4: AC) and their frequency distributions were estimate...
Characterization, Tissue-Specific and Developmental Stage Expression of Somatostatin in Coilia nasus
Siyu Yang1, Fukuan Du2 and Pao Xu1,2*
...yogenesis, SS1 mRNA was detected during early-stage embryonic development, decreased during subsequent developmental stages then increased gradually from the stage of midgastrula onward. This study provides some basic evidence that SS1 may play a role in growth, development and metabolism in C. nasus, and provides a basis for further study of SS neuropeptide family in C. nasus....
Ecevit Eyduran1, Daniel Zaborski2*, Abdul Waheed3, Senol Celik4, Koksal Karadas5 and Wilhelm Grzesiak2
...e automatic interaction detector (CHAID), radial basis function (RBF) networks and multilayer perceptrons with one (MLP1) and two (MLP2) hidden layers in body weight (BW) prediction from selected body measurements in the indigenous Beetal goat of Pakistan. Moreover, the results obtained with the data mining algorithms were compared with multiple linear regression (MR). A total of 205 BW records including one categorical (sex) and six continuous (head girth abo...
Muhammad Sohail1,*, Muhammad Naeem Khan1, Naureen Aziz Qureshi2 and Abdul Shakoor Chaudhry3
... parameters selected to detect DNA damage. Low doses (120 µg L-1) of each metal induced significantly higher levels of DNA strands breaks as compared to medium dose (240 µg L-1) and very low levels of DNA damaged was observed at high dose (360 µg L-1). Cu and Pb showed significantly higher value of % of tail DNA (56.74±1.81, 47.36±1.23) and comet tail length (41.30±0.758, 49.15±1.90), respectively, as compared to C...
Tasnim Farasat*, Saima Sharif, Farkhanda Manzoor, Muneeza Zafar and Shagufta Naz
Abdul Sattar Baloch1*, Abdul Rasheed1,2, Rahmatullah Rind1, Jam Kashif Sahito1, Rehana Buriro1, Muhammad Faisal Ayoob1 and Parkash Dewani1
...mples were collected to detect the antibodies against the disease, however, none of the sample was found to be positive for brucellosis. It was concluded that the brucellosis is prevalent among camels of studied areas....
Hira Akhter1, Bilal Aslam2*, Naveed Shahzad3, Tanzeela Farooq4, Muhammad Umer5 and Muhammad Hidayat Rasool2
...ruses and its molecular detections in asymptomatic commercial layers from Faisalabad district of Punjab, Pakistan. Overall 120 blood samples, 24 tissue samples from each organ (trachea, lung and intestine) were collected from the 12 commercial layer flocks selected randomly at the age of 35-50 weeks without any direct clinical manifestation of avian influenza. Serum collected from 120 birds was tested for antibodies against H9N2 by using Haemagglutination Inhi...
Hussain Ahmad1, Inamullah1, Ijaz Ali2, Tauseef Ahmad3, Muhammad Tufail4, Kabir Ahmad5 and Bibi Nazia Murtaza3,6*
...ted and brucellosis was detected by Standard Plate Agglutination Test (SPAT) and Serum Tube Agglutination Test (STAT) and later on confirmed by PCR. Disease was detected in 3.66% (11/300) individuals by SPAT and 2% (6/300) by STAT and confirmed in 2.66 % (8/300) by PCR. The prevalence of disease in females was found to be higher as compared to males (in current studied group). The age group 31-40 years, was found to be more...
John G. Bruno
...to a greater ability to detect these serotypes if coupled with genetic identification techniques such as PCR or gene probe hybridization....
Oladipo Elijah Kolawole, Oloke Julius Kola and Awoyelu Hilda Elukunbi
Nighat Sultana1,2,*, Shazia Iftikhar1, Nafeesa Qudsia Hanif2 and Iffat Tahira2
...ively. Aflatoxin B2 was detected in only two samples (16%) (1.2 and 1.3ng/g), aflatoxin G1 and G2 were not found. Ochratoxin A was found more frequently in fresh (54.16%) than in ensiled (20.86 %) samples with a mean of 8.06 and 4ng/g respectively. Average values for total aflatoxins (TAFs) and OTA were below the permissible limits as per regulation of European commission (EU), i.e. 20ng/g and 10ng/g, respectively....
Shazia Irfan1*, Asima Rani1, Muhammad Arshad2 and Razia Bashir1
...atients were genotyped. Detection of rs2070424 polymorphism was carried out using allele specific PCR based amplification strategy. The serum samples were analyzed for determination of metals and minerals through Atomic absorption spectrophotometer (AA 6600 Shimadzu). The statistical analysis indicated that non-significant association existed between SOD1 (rs2070424) gene polymorphism and RA (p>0.05). Results of HWE estimation indicate that allele frequenci...
Aziz-ul-Rahman, Farooq Yousaf, Naveed Anwar and Muhammad Abubakar
Kiran Afshan1*, Sarwat Jahan1 and Mazhar Qayyum2
...t assay (ELISA) for the detection of IgG against fascioliasis in sera of small ruminants. The result of current study indicated diagnostic accuracy of test 95.6%, while sensitivity and specificity of this assay for small ruminants was 97.83% (95% CI: 92.35% to 99.67%) and 93.75% (95% CI: 87.54% to 97.44%) respectively. In field this test indicated significant (p<0.05) values for sheep as compared to goat indicating high risk of infection in sheep. The curre...
Farkhanda Manzoor1, Iram Amin2,*, Muhammad Shahid2,Samia Afzal2,Hania Ramzan1, Tahir Rehman Samiullah2 and Muhammad Idrees3
...ested by nested PCR for detection of dengue virus. Out of total 50 pools of Aedes aegypti larvae, DV-2 was the most prevalent serotype. DENV-1 was not found in any pool. While all pools of Aedes albopictus were negative for dengue virus. Our study showed that the main cause of spreading of dengue virus was Aedes aegypti. Serotype DENV-2 was dominant in the field collected larvae of Aedes aegypti in Lahore and Sheikhupura, Pakistan.&...
Pam D Luka, Frank N Mwiine, Bitrus Yakubu, Joseph Erume, Ricardo Pérez-Sánchez, Hermann Unger and David Shamaki
Jehangir Khan1,2*, Inamullah Khan3, Ijaz Ali2, Aqib Iqbal2 and Muhammad Salman4
...y tyres trade. DENV was detected in Aedes aegypti (57%) and Aedes albopictus (43%) adults (n=1050) and larvae (n=550). The samples were collected from four (Shuba Bazaar, Tarnab Farm, Wazir Bagh and Hayat Abad) dengue suspected locations of district Peshawar, Khyber Pakhtunkhwa (KPK), Pakistan. A semi-nested multiplex PCR was used to amplify the cDNA of the four dengue serotypes. Six out of 35 (17.14%) adult mosquito pools were found positive for...
Jiancheng Zhai, Li Gao, Yanling Xia and Heping Li*
...h sexes. The genes were detected with three anchored primers and ten random primers using differential display reverse transcription PCR (DDRT-PCR). Five differentially expressed fragments were obtained through recovery, cloning and sequencing. Three of them (Af1, Ce1, Ci2) presented a higher expression in antler mesenchyme of female reindeer, but the other two (Ae1, Af3) were the opposite. The results of Blast in GenBank showed that they shared high homologie...
Mingqin Shao1*, Bin Chen1, Peng Cui2, Binbin Zeng1 and Jianhong Jiang1
...nificant difference was detected in the daily travel distance between different months (F = 0.658, P > 0.05). Significant differences were detected in the daily home rang between January and February, and between February and March. No significant correlation was observed between daily travel distance and home range (R = 0.256, n = 12, P > 0.05). The significant correlation between daily home range and da...
Zafar Iqbal1* and Muhammad Khurshid2
...tential to expedite the detection of plant viruses from plant species containing various forms of PCR amplification inhibitors. In current study, IC-PCR was used for the detection of Tomato leaf curl New Delhi virus (ToLCNDV) from plant extracts of Nicotiana benthamiana plants inoculated with ToLCNDV. To immunocapture the ToLCNDV, two antisera raised against Tomato yellow leaf curl virus-coat protein (TYLCV-CP)...
Akbar Ali1,*, Khalil Mohamed2 and Fawzia Toulah3
...ii IgG and IgM were detected in serum by ELISA method. Toxoplasma gondii DNA was detected using PCR. Most women were in the 20-29 years age group (48%), followed by 30-39 years (33%) and >40 years (14%). Overall prevalence of Toxoplasma gondii IgG was seen in 12%, with significantly more in women with age more than 30 years as compared to those with age less than 30 years (19% vs 8%; p=0.038). The...
Abdul Wajid Khalil and Zafar Iqbal
...e Cadmium metal was not detected while lead was found in permissible limits in both the aerial parts and roots of B. procumbens. The aspartic acid (31.25±0.08g/100g), Glutamic acid (25.27±0.06 g/100g), Alanine (12.74±0.12g/100g) was found in higher concentration in aerial parts than roots while some essential amino acid, Tryptophan (6.23±0.06g/100g), Arginine (3.49±0.08 g/100g), Phenylalanine (1.77±0.08 g/100g) were re...
Idowu Fagbamila1*, Adaobi Okeke2, Micheal Dashen2, Patricia Lar2, Sati Ngulukun1, Benshak Audu1, David Ehizibolo1, Paul Ankeli1, Pam Luka1, Maryam Muhammad1
Cansu Akbulut1,*, Tuğba Kotil2, Burcu Öztürk1 and Nazan Deniz Yön1
...ization of cristae were detected.Here we showed that TiO2 exposure trigger paraptotic type cell death in zebrafish ovary.
...
Saqib Ali1, Suliman Ali1, Lina1, Wen Zhou1, Muhammad Irfan Waris1, Ashfaq Ali2 and Man Qun Wang1,*
...y stage of intestation, detection of Anoplophora glabripennis and exposure will help to eliminate the pest in addition to prevent its establishment. Plantation with different tree species, the cultivation of fast-growing timber forest, the plantation of trap trees, sanitation, removal of the damaged trees and exact placement of insecticide saturated sticks into larval sites can reduce the spread of ALB in the regions of China. The ecological, in additio...
Wali Khan*, Ghazal Mumtaz, Saima Bibi and Salma Afzal
...olytica cyst 28.5% were detected. The highest prevalence was recorded for Entamoeba histolytica while Trichuris trichura was detected as the least parasitic infection. Coriandrum annum (Coriander) was found highly contaminated 14.2% with parasitic infection while Zingiber officinale (Ginger) with least 1.78%. The vegetable collected from lower Dir were highly infected 41(73.2) than the upper Dir district 15(26.7). Based on t...
Muhammad Shahid1, Iram Amin1,*, Samia Afzal1, Zareen Fatima1, Sadia Zahid1, Usman Ashraf1 and Muhammad Idrees2
Tausif Ahmad1, Iahtasham Khan2, Saddaf Razzaq1, Saeed-ul-Hassan Khan1,* and Raheela Akhtar3
...to test its efficacy in detecting Brucella in blood of infected animals. Initially a Brucella genus-specific bcsp31 genomic region based rPCR was used. This was followed by two species-specificrPCRs that detected IS711 genomic region of B. abortus and B. melitensis. Five (8.3%) serum samples from cattle and 1 from buffalo (1.6%) were found to be positive for B. abortus by RBPT. Four ...
John G. Bruno
...ens to enable sensitive detection of rickettsia exposure in humans who have seroconverted. Such anti-idiotypic aptamer antigens might also serve as rickettsial vaccines, if fully developed. The top five highest affinity R. rickettsii and top five R. typhi anti-Fab aptamers as assessed by enzyme-linked aptamer sorbent assay (ELASA) screening were shown to specifically bind their cognate antibodies when captured by their Fc tails on the surface of Protein A-conj...
Wesam Hasan Mohamed Mady 1*, Bing Liu2, Dong Huang2, A. Arafa1, M.K. Hassan1, M.M. Aly1, Pucheng Chen2, Yongping Jiang2* and Hualan Chen2
...accination for antibody detection by HI test. All immunized chickens shown high HI antibody titers (9Log2) two weeks post-booster dose. The chickens were then challenged using homogenous AI H9N2 strain. During the course of infections, oropharyngeal and cloacal swabs were collected from all chickens at 3, 5 and 7 days post-challenge (p.c.) for virus titration in eggs. Data indicated that all chickens vaccinated with pCA-Egy-H9 were fully protected without clin...
Muhammad Asif Zahoor1, Zeeshan Nawaz1, Abu Baker Siddique1, Sajjad ur Rahman2 and Shahid Ali1*
...e present study we have detected Mycoplasma spp. from tissue samples collected from clinically sick (60 samples) and apparently healthy broiler birds (30 samples) in a commercial farm, using classical isolation and compared with molecular identification of Mycoplasma infections. A total of 96 tissue samples from trachea, air sacs, lungs and oral swabs along with blood from each bird were collected and initially subjected to isolation of Mycoplasma spp. using b...
Tianzhu Chao, Huiqiang Cai, Yuxun Zhou, Kai Li* and Junhua Xiao
...e clades, only CAS1 was detected in all the samples from mainland China. M. m. musculus exhibited no broadly distributed clade, which might be explained by frequent human-assisted movements. Our study offers a newly detailed perspective on the subspecies distribution of M. musculus and its history of migration to eastern China.
...
Matiyar Rahaman Khan1,*, Sabyasachi Pal2, Ghule Tushar Manohar2, Somnath Bhattacharyya3, Amit Singh4, Prahlad Sarkar5 and Samuel Lalliansanga6
Nirbhay Kushwaha, Achuit K Singh, Brotati Chattopadhyay and Supriya Chakraborty
Recent advances in geminivirus detection and future perspectives
...xt-align: justify;">The detection and identification of viruses has been a challenge since the advent of the discipline of plant virology over a century ago.Agreat variety of methods have been developed that permit differentiation of viral pathogens.These methods, initially based solely on identifying the distinct biological characteristics of different viruses, were soon supplemented with methods based on light or electron microscopy and serology and subseque...
Bijan Kumar Das
Incidence of Aleurocanthus spp. (Aleyrodidae: Hemiptera) on betelvine (Piper betle L.) and their interaction with host plants
... recorded in this area. Detection of host resistance against insect pests is very relevant for genetic improvement programmes. Till date, scanty information is available on the source of tolerance, if any, against betelvine blackfly. The reaction of some betelvine cultivars to A. rugosa was evaluated in the boroja with different varietal collections from different parts of India. None of the entries under the purview was completely free from infestation. A few...
Muhammad Mansoor, Zaigham Abbas and Nageen Husssain*
...nd applied procedure to detect minor changes in paw swelling, ANA autoantibodies, CCL11, C3c, glucose level and renal damage. We detected increased symptoms of arthritis and gastrointestinal tract involvement in gluten containing diet group compared with standard diet disease control group. ANA autoantibodies, C3c and renal damage between gluten and standard diet group was non-significant. The remission of SLE manifestations...
Hussein Aly Hussein1*, Omneya Mohamed Khattab2, Shereen Mohamed Aly2, and Mohammed Abdel Mohsen Rohaim1
...cally to the previously detected LSDVs from infected ruminants, indicating a potential role of Ixodid ticks for transmission of LSDV. This study showed the role of A. hebraeum ticks for transmission of LSDV. So, tick control is a crucial part, which should be included as a part of LSDV control measures in endemic countries....
Nasar Khan1,2, Fahad Ali3,7, Sulaiman Bahadar5,*, Khalil ur Rahman3, Abdul Aziz3, Noor ul Akbar4, Muhammad Daud6, Azam Hayat5 and Mujaddad ur Rehman5
...0 years. HBV genome was detected in 79.21% of HBsAg positive samples, showing that HBsAg on ELISA is much more sensitive than PCR as HBV shows chronic conditions and both of the tests are needed against HBV.
...
Muhammad Ashfaq,1 Shamim Akhtar,2 Saleem Akhtar2,* and Mariyam Masood2
...otocol was developed to detect hyperparasitism in cotton mealybug. Cotton mealybug, Phenacoccus solenopsis (Hemiptera: Pseudococcidae) is an invasive and major pest on cotton. Aenasius bambawalei (Hymenoptera: Encyrtidae) is an effective parasitoid of mealybug and has been the backbone of the biological control program of cotton mealybug in Pakistan, but its multiplication for inundative releases has been impacted by a hyperparasitoid, Promusc...
Javeria1, Masroor Ellahi Babar2,*, Tanveer Hussain2, Rashid Saif2, Sadaf Rashid3, Hanan Sarfraz4, Abbas Ali Shah3, Muhammad Wasim1 and Muhammad Abdullah1
...alysis was performed to detect the possible disease causing effect of genetic variants with OCD. Sequencing of BDNF gene showed one change G>A at position c.196 (rs6265) resulting in a nonsynonymous change from valine to methionine. We found only one homozygous and heterozygous carrier for this polymorphism. Healthy siblings of the carrier individuals and control group were screened and found as negative for this polymorphism. In-silico analysis thro...
Wesam Hasan Mohamed Mady1*, Bing Liu2, Dong Huang2, Abdel Satar Arafa1, Mohamed Khalifa Hassan1, Mona Mehrez Aly1, Pucheng Chen2, Yongping Jiang2* and Hualan Chen2
...that chickens developed detectable HI antibody titers up to 6log2 within two weeks post-booster vaccination. The challenge experiment was performed using isolate A/chicken/Egypt/A6/2011 (H5N1) with concentration of 105 EID50 to evaluate the protective efficacy of the Egy-H5 plasmid DNA vaccine. The Egy-H5 plasmid DNA vaccine induced complete protection (100%) and there was no virus shedding in all chickens immunized with the Egy-H5 plasmid DNA vaccine. Taken t...
Rais Ahmed1,*, Khushi Muhammad1, Masood Rabbani1 and Muhammad Sarwar Khan2
...DNA (bcsp31) was detected using Real Time PCR and prevalence was 2.11%. Out of five species, only two species B. abortus and B. melitensis were detected through conventioneal multiplex PCR. Prevalence of soil borne species specific DNA of B. abortus and B. melitensis was 1.8% and 0.31%, respectively. Out of nine, three districts were positive for Brucella species specific DNA. All di...
Muhammad Aslam Farooqi1,*, Mansoor-ul-Hasan2, Sohail Akhtar1, Muhammad Arshad2, Muhammad Naveed Aslam3 and Muhammad Rafay4
...graphy with ultraviolet detection (HPLC-UV) was used to determine residues of commonly used agricultural insecticides (imidacloprid, thiametoxam, profenofos, endosulfan, spinosad and deltamethrin) in multi-floral raw honey of Apis dorsata F. collected from the cotton belt area of Punjab, Pakistan. The residues of these insecticides were extracted using ethyl acetate. Honey samples were spiked at 0.1 and 0.01 mg/kg. The mean recoveries of these insectici...
Ahmed Zein Elabdeen Mahmoud1, Muaz Magzob Abdellatif2* and Mohamed Abdelsalam Abdalla3
... intestine. PPRV NP was detected in sheep (n=20) and goats (n=18), anti-PPRV NP was also detected among sheep and goats (n=162) by ELISA, 3ABC-FMD enzyme immunoassay were positive (n=6) when ovine sera were tested. The study revealed that PPR-FMD viruses were circulating in the region and warrants proper control measures.
...
Mushtaq Ahmad1,*, Mehboob Ahmed1,2 and Nasim Ahmad1
Fang Wang, Yong-Fang Jia, Po Wang, Qi-Yan Du and Zhong-Jie Chang*
...n the present study, to detect the effects of letrozole on gonad differentiation, 5dph fry of carp were exposed to different concentrations of LET. At 130 dph, sex ratio was recorded. The results showed that continuous exposure to LET induced the changes of sex ratio. High concentration of LET can promote male-biased sex ratio. In order to reveal the molecular mechanism by which LET caused sex reversal, juvenile carp (120dph) were exposed to 0, 5, 25, 125, and...
Rabia Tahir1*, Khushi Muhammad1, Masood Rabbani1, Muti-u-Rehman Khan2, Farah Khan1, Hassan Bin Aslam1 and Arslan Mehboob3
...esence of AAV-4 DNA was detected through PCR. Chicks from virus-challenged breeders failed to show a clear and expected PCR positivity. The connotations derived from these findings are that vertical transmission via transovarian route is reported the field conditions. However, based on genetic detection such evidences are lacking. The molecular mechanism and establishment of latent infections warrant future investigations to...
Lucas Pedroso Colvero1, Paulo Eduardo Brandão2*, Cesar Alejandro Rodriguez Rosales2, Hanny Swam3 and Laura Yaneth Buitrago Villarreal1
... for vaccine B were not detected, suggesting a role of the poor emulsion quality on the performance of vaccine B. These results demonstrate that effective quality control and emulsion preparation are essential benchmarks of immunization and vaccine potency. ...
Shengyan Su, Zaijie Dong*, Wenbin Zhu, Lanmei Wang and Jianjun Fu
...ect in one group can be detected. Heilongjiang carp ♂ × Jian carp ♀ (Yj) combination had the highest catch-up like growth degree and the Heilongjiang carp ♂ × Heilongjiang carp ♀ (Yy) combination had no compensatory like growth. Reference standard was set as arithmetic progression in 5% each step for the whole group (e.g. 5%, 10%, 15%, etc.) Compared the harvesting weight and weight gain, significant difference...
Faiz Muhammad1,*, Syed Khurram Fareed1, Urooj Zafar1, Taseer Ahmed Khan2 and Aqeel Ahmad1
... novel tPCR approach to detect Mycoplasma gallispeticum, Mycoplasma synoviae and nonpathogenic mycoplasmas at the same in a single PCR reaction collected from diseased chicken. Direct PCR from the clinical samples produced false negative results. Both culture and PCR were combined as culture-enhanced PCR approach. Three different DNA crude preparation methods were used and broth dilution method found simpler and most efficient for positive PCR. P...
Xue-yang Wang1, Shang-zhi Zhang1, Ming-hui Liu2, Dong Yu1, Yan Ma1, Dong-qiong Fei1, Hai-zhong Yu1 and Jia-ping Xu1,*
...M-like protein could be detected by immunofluorescence in all analyzed tissues, including head, midgut, hemolymph, fat body, testis and ovary. More importantly, the relatively high expression level of BmARM-like mRNA were observed in BC9 (near-isogenic line) following BmNPV infection as compared to P50 (susceptible strain), which was further validated in the midgut of A35 (resistant strain). The expression levels of BmARM-like protein showed similar tre...
Carolina A. Bonin1,2, Eric A. Lewallen2,3, Andre J. van Wijnen3, Marta Jussara Cremer4 and Paulo C. Simões-Lopes5*
... socialization. The detection of these key areas should facilitate both local and broad-scale efforts to preserve critical habitats for this population of dolphins, and by extension may help inform management plans for ecologically vulnerable S. guianensis populations in other parts of their distribution.
...
Jianping Li1, Qian Jiang2, Wei Chen2, Yumei Li3, Huaizhi Jiang4, Jinlong Huo5 and Qiaoling Zhang2*
...no acid (AA) change was detected at KIT exon 13 in both the white cashmere goat and the black cashmere goat. Moreover, comparison with other species revealed three dramatic amino acid mutation areas. Our results also indicated that c-kit expression was higher in the white cashmere goat than in the black goat, and this significant difference was detected by q-PCR and western blotting. All cashmere goats of different co...
Hailong Dong1, Hui Zhang2, Kun Li2, Khalid Mehmood2,3, Mujeeb Ur Rehman2, Fazul Nabi2, Yajing Wang2, Zhenyu Chang1,2, Qingxia Wu1,* and Jiakui Li1,2,*
...in reaction was used to detect representative virulence factors or genes, including E.coli adherence factor (K88, CS31A, afaE-8), toxins (estA, estB, Stxl, Stx2, EAST1), pathogenicity island (eaeA, irp2, ETT2) and outer membrane protein (ompA). Moreover O-antigen serotype was tested by slide agglutination test and a mouse model was built to assess the lethality of the E.coli isolates through subcutaneous-infecti...
Wali Khan1,*, Noor-Un-Nisa2 and Aly Khan3
...i> 2.77% (n=7/252) were detected in order of their prevalence. The adults were found marginally more parasitized than children (P<0.05). The females were more infected than males with (P<0.05). No significant association was found among the occupational groups and parasitic infection (P<0.05). However, shepherds were found more infected than farmers and education concerned. Due to comparative based approach in different occupational communities the pr...
Bacillus subtilis BML5 Isolated from Soil Contaminated with Poultry Waste has Keratinolytic Activity
Aqsa Imtiaz and Abdul Rehman*
.... A 130-kDa protein was detected from feather-treated bacterial cells, suggesting its role in degrading feather. B. subtilis BML5 can be used to overcome the poultry wastes based environmental pollution and keratinase could be used to improve feather meal digestibility in animal feed as an additive.
...
İbrahim Aytekin1,*, Ecevit Eyduran2, Koksal Karadas3, Rifat Akşahan4 and İsmail Keskin1
...s and especially in the detection of ideal fattening period for each breed under the condition.
...
Saleh S. Alhewairini
...ed and they only showed detectable reduction on their movement in both field treatments. Finally, this study would recommend the use of oxamyl in managing and controlling the infestation of RPW.
...
Lihong Qin1, Guoliang Zhang1, Chaojie Zhu1, Jian Wu1, Zhihui Zhao2,* and Yumin Zhao1,*
...and protein levels were detected after bull testicular Sertoli cells were transfected with miR-122 and miR-449a. Meanwhile, DUSP4, PDK4 and FKBP1B overexpression experiments were conducted. Eventually, MTT assay was performed to observe the cells proliferation. The results showed that miR-122 and miR-449a were highly-expressed in testis tissues (p<0.01). The expression levels of miR-122 and miR-449a in the 24-month-old testes were highe...
Bian Xunguang1,2, Li Jiancheng1, Xu Chu1 and Yang Li2,3,4,*
... of AR in the cells was detected by immunohistochemistry. The results showed that the expression of AR mRNA was found in the infundibulum, magnum, isthmus, uterus and vagina of the oviduct. The expression of the AR mRNA in infundibulum was the strongest, and the vaginal expression was weak. The immunohistochemical results was consistent with the molecular expression of AR mRNA, the positive reaction in the infundibulum, isthmus and uterus is more intense, foll...
Yakut Gevrekçi* and Çiğdem Takma
...d Automatic Interaction Detection (CHAID) algorithms. A total of 1912 EP records of layers which hatched at four different periods were collected from a commercial flock in west of Turkey. The analysis performed with cathegorical (cage and line) and continuous (hatch, age at sexual maturity and body weight of sexual maturity) predictors and a continuous response variable (EP). The Spearman’s rho correlation coefficients among all predictor variables were...
Muhammad Jamal Nasir*, Anwar Saeed Khan and Said Alam
...e ability of farmers to detect climate change and how they have adapted to whatever climate change they believe has occurred. Farmers have indigenous knowledge of the climate of the area to predict and forecast the changing weather and climate. Knowing such traditional knowledge provides an opportunity to understand how the farmers are adapting to new type of weather. The study was carried out in Balambat Tehsil District Dir Lower. They study is primarily base...
Tahira Kamal1*, Saeed-Ul-Hassan Khan2, Amir Bin Zahoor3, Khalid Naeem3, Muhammad Naeem Riaz1, Siddra Tayyab Akhtar4, Ghulam Muhammad Ali1*
...are developed for rapid detection of FMD but these are expensive and time consuming. Elisa, cell culture, Reverse Transcriptase (RT-PCR) and real time PCR need specific high quality equipments in lab. Whereas Lamp PCR is simple and accurate test for the rapid diagnosis of FMD. This study has been done initially with FMD known serotypes (O, A, Asia1) already in use at animal health.Viral RNAs were extracted using R Neasy Minikit (Qiagen) according to instructio...
Wali Khan1,*, Noor-Un-Nisa2 and Muhammad Asif Nawaz3
.../i> 0.89%(n=4/447) were detected in order of their prevalence. The individuals below 15 years of age were found marginally more parasitized than above 15 years (0.3856, P<0.05). Males were more infected than females with (0.3157, P<0.05). No significant association was found among the occupational groups and parasitic infection (0.3089, P<0.05). However, shepherds were found more infected than farmers and education concerned. Due to comparative based ...
Yanjie Guo1, Weini Wu1, Hongmei Wang2, Xiao Guo2 and Yongping Xu2,*
...n situ hybridization to detect the expression and distribution of IL-18Rα in the inferior mesenteric ganglion of the female goat. Our results showed that IL-18Rα mRNA and proteins are expressed in the IMG, which provides molecular evidence for the study of immune and neuro-modulation in the female reproductive system.
...
Rashid Minhas*, Iftikhar Ahmad Khan**, Faisal Saeed Awan**, Lal Hussain Akhtar*, Syed Awais Sajid Shah* and M. Shahjhan Bukhari*
ESTIMATION OF GENETIC DIVERSITY AND HYBRID IDENTIFICATION IN AMERICAN COTTON (GOSSYPIUM HIRSUTUM L.) BY PCR BASED RAPD ANALYSIS
...6.88%. The polymorphism detected among the genotypes was employed as marker for hybrid identification. Comparing the banding patterns of RAPD of the parents with their particular hybrids, true hybrids were identified by six primers namely GLA-7, GLA-8, GLA-15, GLA-19, GLD-3 and GLD-5. Consequently, RAPD analysis can be used as a marker technique to ascertain the hybrid nature of offsprings and to evaluate parental lines in terms of genetic diversity....
S. Talat Gilani*, Shahid Hameed* and Hussain Shah*
OCCURANCE AND DISTRIBUTION OF POTY VIRUSES INFECTING GARLIC IN PAKISTAN
...e study was designed to detect and determine the prevalence, incidence and distribution of the poty viruses causing diseases in garlic (Allium sativum) from major garlic growing areas of Pakistan. The yellow stripes, mosaic and chlorotic spot symptoms of the disease resemble the viral infection in garlic reported to occur worldwide. Altogether 690 samples were collected from 29 locations of Punjab and 40 locations of Khyber Pukhtunkhwa to determine the prevale...
Youbao Zhong1, Xianlai Zhang1, Xiaofen Hu1 and Yong Li1, 2,*
... intestine and could be detected at four layered structures in the small intestine, but were mainly distributed in the lamina propria and the submucosa, surrounded with connective tissue and blood vessels. In conclusion, the mast cells, goblet cells, lymphocytes and lymphoid nodules contributed to the small intestine mucosal immune barrier, and the distribution characteristics of the mast cells demonstrated great significance for the hedgehog’s immune sy...
Selçuk Kaplan1,* and Sertaç Atalay2
...he IGF1 gene was detected by amplification of the 294 bp region using specific primers and cleavage with the BfoI enzyme. Allele frequencies of A and B were found with 0.915 and 0.085 respectively. The genotype frequencies of IGF1 gene were 0.85 (AA), 0.13 (AB) and 0.02 (BB). The SNP in the exon 3 of the LEP gene was detected by amplification of the 494 bp region using specific primers and cleavag...
Aitezaz Ahsan1*, Muhammad Usman1, Ihsan Ullah2, Aamer Bin Zahur3 and Adnan Rasheed Malik4
...though these assays are detecting PPRV with precision but there is need to develop some cost effective diagnostic assays which would be applicable in the field conditions and in the developing countries like Pakistan.
... 
