The Expression and Distribution of MIF, ERK, Akt and Rb/E2F in Pulmonary Hypertension in Broilers
The Expression and Distribution of MIF, ERK, Akt and Rb/E2F in Pulmonary Hypertension in Broilers
Yanmei Wang1,2, Haoyun Li1, Lijuan Han1 and Wenkui Wang1*
ABSTRACT
Pulmonary hypertension (PH) is a metabolic disorder that occurs in broilers. It is characterized by pulmonary hypertension, right heart failure and a large amount of yellowish liquid accumulated in the body cavity, and has become one of the most causes of non-infectious death in broiler industry worldwide. The macrophage migration inhibition factor (MIF) and related protein and gene expressions in its downstream signal pathways need to be studied to explore the mechanism of MIF participating in PH. In this study, 4-5 weeks broilers with clinical PH were collected as experimental (PH) group, while the healthy broilers were taken as control group. The related mRNA expression levels were determined by qRT-PCR. The protein expressions and distributions were detected by Western blotting and immunohistochemistry. The results showed that, the mRNA expression of MIF, Raf, ERK, Akt, GSK3β, cyclin D1, Rb, E2F in PH group were significant higher than that of control. Moreover, the expression levels of MIF, p-Raf, p-ERK, p-Akt, p-GSK3β, cyclin D1, p-Rb, E2F proteins in PH group were significantly higher than that of the control. It can be concluded that, MIF participated in the occurrence of PH, possibly through Raf/ERK and Akt/GSK3β pathways by increasing the expression of cyclin D1, phosphorylation of Rb, and transcription function of E2F, thereby induced cell proliferation, caused pulmonary artery vascular remodeling, and lead to PH. This study aims to provide theoretical basis for finding new therapeutic targets for broiler PH.
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