ESTIMATION OF GENETIC DIVERSITY AND HYBRID IDENTIFICATION IN AMERICAN COTTON (GOSSYPIUM HIRSUTUM L.) BY PCR BASED RAPD ANALYSIS
Rashid Minhas*, Iftikhar Ahmad Khan**, Faisal Saeed Awan**, Lal Hussain Akhtar*, Syed Awais Sajid Shah* and M. Shahjhan Bukhari*
ABSTRACT
DNA of 15 cotton genotypes comprising five parents and their ten F hybrids was extracted successfully from their immature leaves to estimate 1 genetic diversity and hybrid identification. Out of the 26 RAPD primers used for DNA amplification, 14 primers produced polymorphic bands, while 12 produced monomorphic bands. These 14 primers yielded total 96 RAPD markers, out of which only 40 were found polymorphic which resulted in 41.66% polymorphism among the genotypes studied. The number of RAPD markers produced ranged from 4 to 12. Average number of 6.40 bands per primer was observed. On the whole, 87.50% genetic identity was found in material under investigation and it ranged from 68.07% to 96.88%. The polymorphism detected among the genotypes was employed as marker for hybrid identification. Comparing the banding patterns of RAPD of the parents with their particular hybrids, true hybrids were identified by six primers namely GLA-7, GLA-8, GLA-15, GLA-19, GLD-3 and GLD-5. Consequently, RAPD analysis can be used as a marker technique to ascertain the hybrid nature of offsprings and to evaluate parental lines in terms of genetic diversity.
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