Impact of Polyethylene Glycol on Newly Established Ovarian Cell Line (Sl-Omi) from Leaf Worm of Spodoptera Littoral's and on Viral Infection
Impact of Polyethylene Glycol on Newly Established Ovarian Cell Line (Sl-Omi) from Leaf Worm of Spodoptera Littoral's and on Viral Infection
Khamiss l , Omaima; El Helaly , Alexandra and Abol Ela 3, Said
ABSTRACT
New cell line from the Egyptian cotton leaf worm ( Spodoptera littoralis ) originated from the insect ovaries tissues was established, cloned and maintained as contentious cell line. Nine serial dilutions of Polyethylene glycol (PEG) were prepared from mother suspension (18.2g PEGS" in 100 ml double distilled de-ionized water) started fromlO 1-109. Each concentration was prepared to treat five replicates of C35 cell dish. Treated cells were observed daily and counted 5 times within 15 days, and cell viability was detected by using the viability staining (Trypan blue). Mother suspension treatment reduced cell number 30 minutes to 24 hours post application. The concentrations of 104, 10-5 and 10-7 PEG which '.vere selected by the screening test were the most effective concentrations on increasing cell numbers respectively but the concentration of 104 was the most effective comparing with the untreated control. All concentrations from 10-3 to I o-9 supported cell growth rate more than control non-treated cells. PEG concentrations froml to 10 proves that PEG improve the cell proliferation and decrease cell death .An infection with Sl NPV baculovirus (Nucleopolyhedrovirus) was carried out on (SL Omi) new established cells in the presence of PEG treatments which demonstrate that PEG has affected the penneability of cells to the NPV infection and enhances the viral replication.
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