Chicken Embryo Fibroblasts are Effective Cells to Study the Function of Chicken Neurexophilin 1 Gene
Chicken Embryo Fibroblasts are Effective Cells to Study the Function of Chicken Neurexophilin 1 Gene
Dongmei Sun, Meiling Chen, Hafiz Ishfaq Ahmad, Yun Liu, Xunping Jiang and Guiqiong Liu*
ABSTRACT
Neurexophilin has been proved to be a differentially expressed gene in chicken sperm storage tubules. In this work, Immunocytochemistry, immunofluorescence and Western-blot were performed to detect the expression of neurexophilin 1 in epithelial cells of chicken utero-vaginal junction, chicken embryo fibroblasts (DF-1 cells) and Hela cells. The immunocytochemistry results showed that neurexophilin 1 was expressed in both the epithelial cells of chicken genital tract and DF-1 cells. Immunofluorescence revealed that neurexophilin 1 was expressed in cytoplasm as well as cell nucleus of chicken fibroblasts and Hela cells. Western-blot analysis displayed neurexophilin 1 in the cell and cell culture fluid of DF-1 cells and Hela cells, and higher expression of neurexophilin 1 in cell culture fluid of DF-1 cells than that of Hela cells. These findings suggested that DF-1 cells are effective and a better choice than Hela cells to do researches on the function of neurexophilin 1 gene.
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October 2018
Vol. 50, Iss. 5, Pages 1601-1998
