Cloning and Bioinformatics of CTSD Gene and its Expression at the Onset of Puberty in Duolang Sheep
Cloning and Bioinformatics of CTSD Gene and its Expression at the Onset of Puberty in Duolang Sheep
Qingjin Li1,2, Zhiyuan Sui1,2, Jihu Zhang1,2, Zhishuai Zhang1,2 and Feng Xing1,2*
ABSTRACT
Recent studies have demonstrated that CTSD gene plays a role in the regulation of reproduction in mammals. However, the role of CTSD gene in the onset of sheep puberty remains unknown, the cDNA sequence of CTSD from Duolang sheep was cloned and sequenced. The expression levels of CTSD were detected in the hypothalamus, pituitary, ovary, uterus and oviduct in the three development periods of prepuberty, puberty, and postpuberty. The results showed that the coding region of CTSD gene of Duolang sheep had a full length of 1,239 bp, encoding 412 amino acids. The sequence of Duolang sheep CTSD was relatively similar to those of other mammals and was in line with evolutionary relationship. The mRNA of CTSD gene was expressed in all the tissues of Duolang sheep at the prepuberty, puberty, and postpuberty. The expression level of CTSD in the hypothalamus and uterus was relatively low. But its expression level in the ovary at puberty was significantly higher than that in other tissues. These results suggest that CTSD may play an important role in the onset of puberty in sheep by regulating the development and ovulation of follicles in the ovary.
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