Galina N. Leonova1*, Ilya G. Kondratov2, Sergei I. Belikov2
...in was isolated from the blood of a person after a tick bite. Phylogenetic analysis showed that these strains are related to LI/A and LI/K strains isolated in England and Scotland.
...
Oladipo Elijah Kolawole*, Oloke Julius Kola, Awoyelu Elukunbi Hilda, Olasunmibo Oluwatobi Gabriel, Ogunyale Tolulope Temitope
E-mail | koladipo2k3@ yahoo.co.uk
...y Virus in Ogbomoso. The blood sample of consented two hundred and seventy five (275) subjects were obtained and their sera were screened for antibodies to HEV using Enzyme Linked Immunosorbent Assay. Samples were collected over a period of 4 months (September – December, 2014). The mean age and mean CD4+ count of the subjects were 40.14±0.47 years and 387.28±18.91 cells/mm3 respectively. Out of 275 subjects tested, 15(5.45%; 95% confidence...
Rodolfo Villagra-Blanco1*, Gaby Dolz1, Danilo Montero-Caballero2, Juan Jose Romero-Zuniga2
Behzad Hussain1, 2, Abdullah Iqbal2, Muhammad Abubakar2*
... and direct contact with blood of the infected animal. The occurrence of CCHF outbreak in Pakistan is increased quite significantly near Eid-ul-Adha. Haemorrhages are the major signs of this fatal disease. No treatment of CCHF is available currently but Ribavirin antiviral drug is used to cure this disease. There is currently no commercially available vaccine for this disease. It is recommended that the disease can be controlled by adopting preventive measures...
Anna-Maria Andersson*, Ann-Kristin Nyman, Per Wallgren
...odies in serum and dried blood samples collected from mink, a VP2 ELISA (enzyme-linked immunosorbent assay) system was recently evaluated. However, such estimations of disease progression are commonly based on single sampling occasions and it is essential to know if estimated antibody levels vary over a period of weeks or months. Therefore, the aim of this study was to evaluate the short term stability and the long term consistency of t...
Nadeem Khan1*, Arshad Ali Khan1, Mukhtar Ahmad2, Muhammad Nouman2 and Badshah Islam1
Inamullah Khan, Muhammad Subhan Qureshi, Rajwali Khan, Syed Muhammad Sohail, Ijaz Ahmad, Muhammad Shoaib and Asim Ijaz
.... A total of 410 and 202 blood samples were collected from cattle and humans respectively. Total 40 samples of subsistence farms and 30 nomadic herds were analysed. Forty eight farm workers, 40 farm owner 36 abattoirs workers, 15 veterinary assistants, 15 veterinarian and 40 butchers were also screen out. Overall sero-prevalence in cattle was 15.36% on SPAT, 14.39% by i-ELISA and 14.14% through PCR. Higher prevalence was recorded in abattoir shops (31.01%) fol...
Jannatul Noor, Md. Ahaduzzaman, Mir Md. Afzal Hossain, Mohammad Alamgir Hossain, Sardar Abdur Rahim and Md. Samun Sarker
...ustify;"> Ticks are blood sucker, ecto-parasites of a wide range of mammals that enforce major economic threats to the livestock industry throughout the world. The present cross sectional study was conducted to assess the prevalence of tick in goat’s population of three different areas (Kaptai, Kattoli and Chittagong Veterinary and Animal Sciences University) of Chittagong, Bangladesh. A total of 60 goats were examined for tick infestation in three ...
Muhammad Ibrahim, Jawaria Ali Khan, Muhammad Sarwar Khan, Wasim Shehzad, Muhammad Avais, Ali Husnain, Naimatullah Khan, Muhammad Kamran Ameen and Abdullah Iqbal
...an be used for comparing blood values and milk composition of healthy cows with animals under trial. Our study concluded that Vitamin E+ Se and Garlic were the most efficient drugs as 66% cows suffering from subclinical mastitis were recovered by treatment of each of them. Cows suffering from subclinical mastitis showed a 50 % recovery percentage when treated with lemon for five days and 33 percent cows recovered from subclinical mastitis when treated with Bla...
Muhammad Mushtaq, Umer Sadique, Naila Chand, Iftikhar Ahmed, Said Sajjad Ali Shah, Ijaz Ahmad, Imran Ullah and Muqadar Shah
...h lipids contents in the blood. These lipids content can cause the atherosclerosis by blocking the arteries canal which increase the risk factors associated with heart attack. Atherosclerosis is also associated with development of diabetes, high blood pressure and kidney failure. Hypercholesterolemia is mainly caused by high fat diet, obesity and diabetes.Hypolipidemic and hypoglycemic effect of water based infusion of Witha...
John Gachohi, Simon Karanja, Salome Wanyoike, Nduhiu Gitahi, Salome Bukachi, Kenneth Ngure
... Kenya. Human and animal blood and urine samples will be collected at the household level whereas soil and water samples will be collected at the community level. Attempts will be made to isolate Leptospira bacteria from urine, soil and water samples. Antibody detection in animal and human serum samples will utilize Microscopic Agglutination Test (MAT). Study outcomes will include (i) geo-referencing of adverse environmental attributes that support Leptospira ...
Pan-pan Guo1, Wei Liu2, Yan Li2, Rui-yu Ma2, Wang Zaigui1*, Kai Zhan2*, Jun-ying Li2 and Sheng-nan Liu2
...y. We extracted DNA from blood samples and analyzed polymorphisms of HTT gene as wells as possible association with egg production performances including age at first egg (AFE), laying rate (LR), egg production (EP), egg weight (EW), average egg weight (AEW). Two polymorphisms (G50831C and G62976A) were detected and located in exon 42 and intron 54 in HTT gene, respectively. One of them (g. 50831 G>C) was a missense mutation. Genotype and allele frequency a...
Tasnim Farasat*, Saima Sharif, Farkhanda Manzoor, Muneeza Zafar and Shagufta Naz
...h systolic and diastolic blood pressure, cholesterol level and serum insulin were significantly higher (p< 0.05) in proliferative group of diabetic retinopathy, while triglyceride level, HbA1c (%), and low density lipoprotein (LDL) were non-significantly higher (p> 0.05) in diabetic retinopathy groups. To conclude high prevalence of diabetic retinopathy was observed among newly diagnosed diabetic patients....
Hira Akhter1, Bilal Aslam2*, Naveed Shahzad3, Tanzeela Farooq4, Muhammad Umer5 and Muhammad Hidayat Rasool2
...b, Pakistan. Overall 120 blood samples, 24 tissue samples from each organ (trachea, lung and intestine) were collected from the 12 commercial layer flocks selected randomly at the age of 35-50 weeks without any direct clinical manifestation of avian influenza. Serum collected from 120 birds was tested for antibodies against H9N2 by using Haemagglutination Inhibition assay. Calculated geometric mean titers of 5.37 revealed the infectivity of the flock with H9N2...
Atta ur Rehman1, Sher Ali2 and Sajid Malik2*
...(KPK) region, ABO and Rh blood groups record of nine Pashtun populations, i.e., Bannu, Dera Ismail Khan, Hangu, Karak, Kohat, Kurram Agency, Lakki Marwat, South Waziristan Agency, and Tank, was collected. Absolute gene diversity, gene differentiation and genetic diversity were estimated to be 0.01, 0.019 and 0.515, respectively. There were the highest affinities between the samples obtained from Lakki Marwat and Tank districts, whereas the least affinities wer...
Hussain Ahmad1, Inamullah1, Ijaz Ali2, Tauseef Ahmad3, Muhammad Tufail4, Kabir Ahmad5 and Bibi Nazia Murtaza3,6*
Ahmed Samy, Wesam Mady, Naglaa M. Haggag, Samah H. Mohamed, Ebtissam N. AlShamy, M.K. Hassan
...1) in chicken peripheral blood mononuclear cells (PBMC). Hemagglutinin gene sequence analysis of the two strains reveal high similarity with difference close to cleavage site (HARSSR/ GLF) and (PARSSR/GLF) for V3 and RSF/1 respectively. Results of growth kinetics in PBMCs showed that V3 replicated more efficiently comparing to RSF/1 at all-time points. Further, strain V3 induced significant up-regulation of IL-1b and IL-6 at 24 hours post infections (hpi) comp...
Asmaa Hamid1,* and Samia Kalsoom2
...ere monitored for random blood glucose and weight for a period of six weeks. Another three groups of rats with induced hypercholesterolemia were fed the same diet as the diabetic rats and were monitored for total serum cholesterol. It was found that both Bengal gram and chickpea had a significant effect on lowering glucose level and total cholesterol of these experimental animals. Bengal gram however, proved to have a significantly (p<0.05) greater effect o...
Fida Mohammad, O.S. Abdalla, Sheraz Ahmed, Fakharuddin and S. Rajaram
...specially those carrying blood from T. tauschii germplasm were equivalent in performance to superior cultivars used in the study, thus can be utilized in breeding for drought stress by wheat breeders....
Blood Selenium and Vitamin E Levels in Heifers: Regional and Seasonal Differences in Northern Cyprus
Serkan Sayiner1* and Hilal Karagul2
...ish the current state of blood selenium and vitamin E levels in heifers, together with roughage selenium levels in eight livestock regions of Northern Cyprus from two different seasons. We also evaluated whether these concentrations were adequate. Heifer blood, feed samples including concentrate feed or roughage were analysed. It was found that there are significant seasonal differences for each region and regional differenc...
Muhammad Luqman Sohail1,*, Muhammad Sarwar Khan1, Muhammad Ijaz1, Muhammad Avais1, Muhammad Yasir Zahoor2, Omer Naseer1 and Muhammad Usman Saleem3
...rease (P<0.05) in red blood cells (RBC), packed cell volume (PCV), hemoglobin (Hb), mean corpuscular hemoglobin concentration (MCHC) and platelets while total leucocytes count (TLC), neutrophils, eosinophils, basophils, lymphocytes, monocytes, red cell distribution width (RDW) and mean corpuscular volume (MCV) were increased significantly (P < 0.05) which indicated hemolytic anemia. While, serum biochemistry showed significant elevation in alanine transa...
Yang Wang1, Zhide Cheng2, Mengjie Tang3, Haixia Zhou1, Xiaolu Yuan4,Muhammad Aqeel Ashraf5, Shuting Mao1 and Jing Wang1,*
.../i>-isopropyl oxamate in blood was 0.08 mmol/L; 3) in skeletal muscles of the inhibitor group and the control group, the LDH activities were 6.30±0.50 U/mg and 10.01±0.59 U/mg, the contents of LD were 0.28±0.039 mmol/g and 0.84±0.16 mmol/g, and the ATP level were 8.15±1.03 nmol/mg and 12.06±1.23 nmol/mg (P<0.01); 5) the inhibition rates of N-isopropyl oxamate to LDH, LD and ATP were 37.12%, 66.27%, and...
Shazia Ali, Hizb Ullah and Sarwat Jahan*
...re a group of hereditary blood disorders in which or absent beta globin chain synthesis, results in reduced Hemoglobin, decreased RBC production and anemia. Total 300 individuals were divided into 4 groups according to age and gender i.e ≤13 years females, >13 years females, ≤13 years and >13 years males. Height in centimeter, weight in kilogram was measured to calculate BMIKg/m2. Serum ferritin (ng/mL) and hemoglobin (gm/dl) alon...
Sadaf Ijaz Malik1, Kiran Afshan2* and Mazhar Qayyum1
...e tissues, aspirates and blood samples of the same animals were analysed. The infection was found prevalent throughout the year but during post monsoon season it was relatively high. Morphological identification of G. expalanatum was carried out on the basis of size and shape of worm. The hematological and bile biochemical changes were found in two groups. Pathologically significant changes were observed in the infected tissue. The parasites had formed ...
Akbar Ali1,*, Khalil Mohamed2 and Fawzia Toulah3
...sitic infections of warm-blooded animals including man. In most adults it does not cause serious illness, but it can cause serious health problems in infants, pregnant women and immune compromised patients. Abortion is one of the most serious outcomes in pregnant women. In this cross-sectional study, a total of 162 women of child bearing age were included from Rafha city of Saudi Arabia. Demographic data was recorded. Specific Toxoplasma gondii IgG and ...
Idowu Fagbamila1*, Adaobi Okeke2, Micheal Dashen2, Patricia Lar2, Sati Ngulukun1, Benshak Audu1, David Ehizibolo1, Paul Ankeli1, Pam Luka1, Maryam Muhammad1
...organic material such as blood, fat, flesh and excreta. In developing countries like Nigeria where access to portable water is lacking, it is not uncommon to “reuse” rinse water for processing several batches of poultry carcass.Several samples were collected from three poultry slaughterhouses within Jos metropolis at intervals for a period of 6 months to check for presence of Salmonella during various stages of processing. Salmonella was isolated f...
Sung Kwon Park1a, Jin Young Jeong2a, Eun Seok Cho3, Yong Dae Jeong3 and Chang Seok Park4*
... (RNA-seq) in peripheral blood mononucleated cell (PBMC) of piglets, Large White x Landrace crossbreds, infected with live strain PED virus (PEDV). Several gene ontology (GO) and differentially expressed gene (DEG) are also identified by RNA-seq. The analyzed transcriptome provides the 1805 DEGs and gene networks involving cellular processes. Between vaccinated and unvaccinated groups, there were 13 and 17 up- and down-regulated DEGs (2-fold; FDR < 0.05;
Tausif Ahmad1, Iahtasham Khan2, Saddaf Razzaq1, Saeed-ul-Hassan Khan1,* and Raheela Akhtar3
...cting Brucella in blood of infected animals. Initially a Brucella genus-specific bcsp31 genomic region based rPCR was used. This was followed by two species-specificrPCRs that detected IS711 genomic region of B. abortus and B. melitensis. Five (8.3%) serum samples from cattle and 1 from buffalo (1.6%) were found to be positive for B. abortus by RBPT. Four (6.6%) out of the 5 RBPT positive cattle samples were also found...
Salman Khalid1,*, Abdul Rehman2, Mohammad Yasin Amir1, Abdul Rehman1, Imran Sarfaraz1 and Muhammad Asif1
... the effects of ticks on blood parameters and efficacy of different drugs on controlling tick’s infestation in commercial layer, a double blind randomized controlled trial was conducted. For therapeutic trials, the 80 birds (n= 60 infested with ticks, n= 20 healthy birds) were selected and divided into four groups (A, B, C and D), each comprising of 20 birds. Group A and B were treated with Ivermectin @ 0.2mg/Kg orally for two days and Fipronil (Spray at...
Muhammad Asif Zahoor1, Zeeshan Nawaz1, Abu Baker Siddique1, Sajjad ur Rahman2 and Shahid Ali1*
...nd oral swabs along with blood from each bird were collected and initially subjected to isolation of Mycoplasma spp. using brain heart infusion broth supplemented with horse serum. The blood was used for serum plate agglutination assay. Thereafter, the samples were subjected to genomic DNA extraction to detect Mycoplasma spp. using multiplex-PCR. Isolation and identification data showed that a total of 58/96 samples (60.41%)...
Adel Eid Mohamed Mahmoud*
...xtures on digestibility, blood and ruminal parameters, growth performance, meat analysis and feed intake of growing lambs. Fifty one Barki lambs with body weight of 30±0.57 kg; in three groups (17 animals, each). Experimental rations were composed of concentrate and clover hay in a 60:40 concentrate: roughage ratio.There was no effect of bakery by-products inclusion on nutrients digestibility and nutritive values except digestion coefficients of crude p...
YaQiu Liu and ZhiJian Wang*
... and squamous with dense blood capillary network underground, while there were no folds on mucous membrane surface. Distance between blood capillary network and intestinal cavity was 1.95±0.34 μm. Respiration epithelial cells of posterior intestine are a kind of modified squamous epithelial cells which show the ultrastructural characteristics of both types I and II pneumocyte of lung alveoli in mammals. The ...
Beenish Zahid1,*, Asim Aslam2, Zafar Iqbal Chaudhry2 and Raheela Akhtar2
Mahmoud Said1*, Abd El Satar Arafa1, Mohammed A. Rohaim2* and Hussein A. Hussein2
...enotype VII. Comparative blood profiling in vaccinated and challenged, non-vaccinated and non-challenged and non-vaccinated and challenged indicated that MDA for H5N1 gradually decreased from 5.1 to 0.4 log2 and disappeared with in a month. While MDA for NDV remained 2.1 log2 until 28th day of age, the Geometric mean titers (GMT) for H5 were increased from the 7th day of age using both antigens (RE-5 antigen) and (S75/Egy/2015 antigen). Generally, GMT post-cha...
Baohua Chen1,2, Wenzhu Peng3, Jian Xu2, Jingyan Feng1,2, Chuanju Dong1,2 and Peng Xu2,3,*
...tine, gill, liver, skin, blood and muscle of common carp. Expression profiles provided us more evidences to understand GST gene functions as well as their functional evolution post duplication. Overall, the whole set of GST genes provide essential genomic resources for future biochemical, toxicological and physiological studies in common carp.
...
Rafi Ullah1, Sarzamin Khan1,*, Abdul Hafeez1, Asad Sultan1, Nazir Ahmad Khan2, Naila Chand1 and Naseer Ahmad1
...t; 0.05), however, other blood parameters and carcass characteristics indicated no significant differences (P > 0.05). Cost per kg of feed gradually decline with increasing dietary level of SCM inclusion levels (P < 0.05), indicating higher economic benefit. However, the gross return per bird and profit per kg meat were higher for diet D4 (P < 0.05). It was revealed that replacement of soyabean meal with silkworm meal did not affect broiler performanc...
Mutassim M. Abdelrahman1,*, Riyadh S. Aljumaah1, Moez Ayadi1 and Shabana Naz2
... Zn, Co and Se levels in blood of ewes treated with bolus with a significant increase in phosphorous (P), Co and Se in the blood of their newborn lambs. Furthermore, a significantly higher (P<0.05) inorganic matter percentage in colostrum was found in treated group compared to the control. The body weight of lambs in treated group at 30 and 60 days was significantly (P<0.05) higher when compared with the control. In co...
Viram Kumar1,*, Moolchand Malhi1, Saeed Ahmed Soomro1, Toufique Ahmed Qureshi2, Muhammad Nawaz Sanjrani3 and Khushal Das Malhi1
... Pakistan. A total of 60 blood samples were collected from peafowl (30 males and 30 female) in months of March and April, 2015. The gender difference showed that RBC, MCV and MCH concentrations were significantly higher (P < 0.05) and the serum total protein and Mg concentrations were significantly lower (P < 0.05) in male compared to female peafowl. In conclusion, the present study provides base-line values for hematology and serum biochemistry of appar...
Furukh Faiz1,2, Muhammad Issa Khan2, Masood Sadiq2 and Haq Nawaz3
...mic Halal Method and the blood samples of the birds were preserved for antioxidant enzymes assay analysis. The data showed that as the citrus waste percentage in the feed of the broiler was increased, its feed intake and the body weight decreased but the citrus waste has non-significant effect on the feed conversion ratio of the broiler birds. The results also showed that citrus waste in the feed have positive effect on the antioxidant status of the broiler bi...
Madiha Hashmi1, Abid Hussain1, Shafiq ur Rehman1, Farida Ahmed2, Shahbaz Aslam3,Nadeem Afzal4 and Zaigham Abbas1,*
Farah Ashfaq* and Tasnim Farasat
..., diastolic and systolic blood pressure, lipid profile and fasting glucose, serum resistin and insulin of three hundred over weight, obese males and females, 17 to 30 years and 100 comparable control subjects were included. Serum resistin and insulin, fasting glucose, triglycerides and cholesterol were found significantly high in overweight and obese groups as compared to the normal weight group (p<0.01). In overweight group, resistin levels were significan...
Sogolo L. Lebelo1,* and Gerhard van der Horst2
...ted with large and small blood vessels and abundant loose connective tissue. Leydig cells have large spherical or slightly elongated nuclei, which possess prominent nuclei and frequently deep indentations of nuclear envelope. The nuclei are characterized by patches of euchromatin and heterochromatin regions with the latter usually occupying the periphery of the organelle. The cytoplasm contains numerous mitochondria containing distinct lamellar cristae, small ...
Ahmed Zein Elabdeen Mahmoud1, Muaz Magzob Abdellatif2* and Mohamed Abdelsalam Abdalla3
...matitis, mucopurulent to bloody nasal/ocular discharges, watery to bloody diarrhea and lameness. The major gross lesions were erosive stomatitis, hemorrhages in lung, liver, abomasum, small and large intestine. PPRV NP was detected in sheep (n=20) and goats (n=18), anti-PPRV NP was also detected among sheep and goats (n=162) by ELISA, 3ABC-FMD enzyme immunoassay were positive (n=6) when ovine sera were tested. The study reve...
Usman Waheed1,*, Humayoon Shafique Satti2, Muhammad Arshad3, Ahmed Farooq3, Abdul Rauf4 and Hasan Abbas Zaheer1,5
Kadir Karakuş1, Bahat Comba2, Abuzer Taş3, Tunahan Sancak3, Arzu Comba2, Devrim Saripinar Aksu4, Hasan Koyun5 and Mohammad Masood Tariq6,*
...sulted in changes in the blood hormone levels, it was determined that these changes did not affect body weight. In conclusion, the evaluation of the process in castrated six-month-old sheep revealed an increase in the body weight which was similar to that in the control group.
...
Ibrahim A. Alhidary1,*, Mutassim M. Abdelrahman1, Riyadh S. Aljumaah1, Abdullah H. Alyemni2, Moez A. Ayadi1,3 and Mohamed Y. Al-Saiady2
Shabana1,*, Farah Ehsan1 and Shahida Hasnain1,2
...pulation. A total of 900 blood samples (diabetic=451, controls=449) were genotyped by PCR-RFLP. A significant difference in genotype distribution was observed between cases (Gly/Gly, 66.9%; Gly/Ser, 20.17%; Ser/Ser, 12.8%) and controls (Gly/Gly, 67.4%; Gly/Ser, 28.5%; Ser/Ser, 4.00%) (p = 0.013). The cases showed significantly higher frequency of the Ser allele compared to the controls (22.9% vs. 18.3%; OR: 1.32, CI: 0.95–1.83, p=0.013). Th...
Ambreen Butt1, Uzma Malik2, Khadija Waheed3, Amna Khanum3, Samar Firdous4, Sara Ejaz5 and Fawad Randhawa2, Tania Shakoori6*
...s diagnosed when fasting blood sugar levels were 95 mg/dL or above. The levels were compared using Mann Whitney U test. Multiple linear regression was used to investigate the association of cobalamin with fasting blood sugar level after adjusting for gestational age and gravidity. ROC curve analysis was conducted to assess the suitability cobalamin as a predictive biomarker for GDM. Median cobalamin were significantly reduce...
Muhammad Usman Qamar1, 2, Sidrah Saleem1, Usman Arshad1, Muhammad Farhan Rasheed3, Hasan Ejaz4, Naveed Shahzad5 and Shah Jahan6,*
Kashif Prince1,*, Muhammad Sarwar Khan1, Muhammad Ijaz1, Aftab Ahmad Anjum2, Atif Prince3, Naimat Ullah Khan1 and Mumtaz Ali Khan1
Zia-ur-Rehaman1, Naila Chand1, Sarzamin Khan1 and Rifat Ullah Khan2,*
Rana Hanan1*, Muhammad Waqar Shafi1, Sumair Anwar2 and Numrah Nawaz1
...ten consent. We recorded blood pressure at three different time points, one minute apart in sitting position after ten minutes of rest. The average of three measurements was considered as final reading. Data regarding related variables was entered and analysed using SPSS version 21. Out of 100 (43 males and 57 females), three students were hypertensive, 55 pre-hypertensive and 42 normotensive; among pre-hypertensive female students were more in number as compa...
Xiaoting Zhang1,2, Jiaxiang Wang1,2,*, Peng Li1,2,* and Lixun Ye1,2
...ere surrounded by a rich blood capillary bed. Ghrelin-ip cells were located among the tertiary bronchus mucosal epithelial cells, respiratory capillary epithelial cells and macrophages. The ghrelin-ip cells showed cytoplasmic staining, they were cone, oval, spindle, round or irregular in shape and tended to be restricted to a single cell. These results demonstrate the presence of ghrelin-ip cells in the lung of the African ostrich and therefore it is speculate...
Faiza Ambreen*1 and Muhammad Javed2
...orpyrifos) in peripheral blood erythrocytes of freshwater fish, Oreochromis niloticus by using Comet assay. The 96-h LC50 value of pesticide mixture was estimated for 180-old fingerlings of O. niloticusin a static system and then four sub-lethal concentrations viz. 1/3rd of LC50, 1/4th of LC50, 1/5th of LC50 and 1/6th of LC50 were calculated ...
Irfan1* , Arshad Javid2 , Muhammad Altaf3 , Muhammad Shahbaz3 , and Khalid Javed Iqbal4
...stems strongly influence blood biochemical profile in M. gallopavo.
...
Muhammad Iqbal Anjum*, Shahbaz Javaid and Mukhtar Ahmad Nadeem
... On 35th day, blood and tibia bone samples from 5 birds per treatment were collected. Total weight gain and dry matter intake in chicks fed rations I, II and III were 1861, 1736 and 2023 gm/bird and 3242, 3284 and 3265 gm/bird, respectively. The chicks fed R-III got 16.00 and 8.00% higher weight gain compared to chicks fed R-II and R-I, respectively. Feed: gain ratio (gm/gm) was found best (P<0.05) in chicks fed R-III (1.61) and poorer in chicks ...
Rabia Qurashi, Qurat ul Ane Gillani* and Furhan Iqbal*
Faiza Hassan1, Mubshara Saadia1,*, Muhammad Sher1, Mian Anjum Murtaza2, Muhammad Arshad3,Asam Riaz4 and Mahmood Ahmad Khan5
...ve thepercent changes in blood glucose levels and lipid profile. Garlic extracts have shown the significant (p<0.001) hypoglycemic activity with no considerable effect on mean body weight of rats (ns, p>0.05) as compared to diabetic rats. The AGE treatment has significantly reduced the blood glucose level (56%), however, the EGE treatment was found effective in normalizing the lipid contents of diabetic rats. The black...
Abd El-Nasser Ahmed Mohammed*
...for analysis of complete blood picture. The results indicated presence of a neovascular capillary network around transplanted ovarian tissues. In addition, visible antral follicles and corpora lutea was demonstrated on the ovarian tissues upon transplantation. Histological examination indicated that transplanted young ovarian tissues restored function better than elder ones. It could be concluded that unilateral ovarian transplantation partially restored their...
Aneeza Jamshed1* and Syed Amir Gillani2
...mission (syringes 88.7%, blood transfusion 91%), signs (effects on liver 92.9%), symptoms (88%), prevention in high prevalent areas (sterilized syringes and screened blood transfusion 86.5%) and management (88.7%). This study showed that it may be an important school health based intervention to educate students about the transmission, prevention and management of hepatitis C, which may help in declining the disease burden. ...
Attia Anwar1, Sajida Malik2*, Zobia Usman1 and Sadia Chiragh3
...ion, serum uric acid and blood cell counts of Sprague-Dawley rats. For this purpose, twenty Sprague-Dawley rats were divided in two groups. Group A (n=10) was given distilled water and Group B (n=10) was supplemented berberine hydrochloride with dose of 75 mg/kg body weight/day. 24 weeks post-treatment, blood samples were collected by cardiac puncture from both groups and tested for Hb (%), WBC, RBC and platelet counts, SGPT...
Muhammad Naeem1, Bushra Sadia1,2,*, Faisal Saeed Awan1and Muhammad Anjum Zia3
...g halo zones. Percentage blood clot lysis led to screening of mutants UV 2, UV 6, UV 7, UV 9, UV 11, UV 12, UV 15 and EB 3, EB 4, EB 11, EB 13 with hyperactivity as compared to wild bacterial strain. Out of the total 15 isolates of EtBr-treated strains, EB4 gave the maximum enzyme activity (7.3 ± 0.005 U ml-1) with a zone diameter of 6 mm. While UV6 was found to be the best UV-induced mutant with an enzyme activity of 20.333 ± 0.006
Sana Qanber Abbasi1*, Ejaz Ahmed2 and Rabia Sattar1
...atients with migraine. A blood sample of 3 cc was drawn from the median cubital vein after securing aseptic measures. The blood sample was allowed to clot for 30 minutes and serum was separated. The serum was then centrifuged andpro BNP levels were estimated by ELISA using Human pro BNP kit. Patients’ history and recorded blood pressure was documented on a questionnaire. The results ...
Maryyum Khalil1, Hamda Azmat1,*, Noor Khan1, Arshad Javid2, Ali Hussain1, Syed Makhdoom Hussain3, Asim Ullah1 and Sumaira Abbas1
...and concentration of red blood cells (RBCs), white blood cells (WBCs), hematocrit (HCT) and numbers of lymphocytes were highest in treatment # 1 with the mean values of 1.29×10ˆ12 L-1, 54.9×10ˆ9 L-1, 18% and 48.7×10ˆ9 L-1. Microbial load was also determined inside and outside of fish as highest microbial load was observed in control group fish on both media nu...
Promy Virk
...T, creatinine levels and blood urea also showed a comparable ameliorative effect of both the bulk Trigonella seed extract (TBN) and the green nanocomposite (TN), being more efficacious than metformin. Thus, the green synthesis and application of the Au/Ag nanoparticles offer a novel approach in nanomedicine for diabetes management.
...
Asad Sultan1, Sahal Ahmad1, Sarzamin Khan1, Rifat Ullah Khan2,*, Naila Chand1, Muhammad Tahir3 and Shakoor Ahmad2
..., feed conversion ratio, blood parameters and nutrient digestibility at both levels of thermal zones. The results of silymarin on growth, nutrient digestibility and hematological parameters were comparatively lower (P<0.05) in broilers during TN and HS zones. The current results indicate that the supplementation of feed with silymarin and ZnO improved the growth performance, blood parameters and nutrient digestibility in ...
Muhammad Kashif Maan1, Muhammad Arif Khan1, Shehla Gul Bokhari1, Muhammad Ijaz2, Hamid Akbar1, Sajid Umar 3 and Muhammad Luqman Sohail4,*
...partate transaminase and blood urea nitrogen as compared to isoflurane anesthesia; whereas, no statistical difference (P>0.05) was observed for alkaline phosphatase, creatinine and bilirubin between the two groups. Heart rate, blood pressure and saturation of oxygen were decreased in both groups but this decline was significantly lesser (P<0.05) in isoflurane administered group compared to xylazine-ketamine administere...
Ghazala Nawaz*, Muhammad Nawaz Malik**, Muhammad Hassan
Mushtaq***, Fraz Munir Ahmad****, Ali Abdullah Shah*****, Farooq
Iqbal******, Shinawar Waseem Ali*******, Zahida Fatima******** and
Amjad Khan*********
SURVEILLANCE OF BRUCELLOSIS IN LIVESTOCK IN RURAL COMMUNITIES OF PUNJAB, PAKISTAN
...was conducted to collect blood samples from small and large ruminants of subtropical rural communities in the province of Punjab during January-March, 2015. Total 59665 sera samples from apparently healthy animals were collected and screened by Rose Bengal Plate test and confirmed by Complement fixation test, respectively. After adjustment for test sensitivities and specificities accordingly, and chi square analysis of results, the true prevalence per 1000 ani...
Youbao Zhong1, Xianlai Zhang1, Xiaofen Hu1 and Yong Li1, 2,*
...th connective tissue and blood vessels. In conclusion, the mast cells, goblet cells, lymphocytes and lymphoid nodules contributed to the small intestine mucosal immune barrier, and the distribution characteristics of the mast cells demonstrated great significance for the hedgehog’s immune system.
...
Fatmagün Aydin1,* and Fatma Burcu Harmantepe2
...al parameters (total red blood cell count, hemoglobin concentration, hematocrit, mean corpuscular volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, total white blood cell count and differential leukocyte counts) showed no significant (P>0.05) differences between the control and all other treatments. Feed intake, specific growth rate, weight gain and final weight were decreased significantly w...
Zhiping Hu, Hussain Ahmad, Jingfei Zhang, Lili Zhang and Tian Wang*
...cantly decreased at 21d, blood urea nitrogen and alanine aminotransferase were significantly increased compared to CON group at 42d in broilers. Serum globulin content (21d) and total protein content (42d) were lower in T2 group than that of CON group. Serum total antioxidant capacity was higher and malondialdehyde content was lower in T1 group than that of CON group at 21d. Serum glutathione peroxidase enzyme activity was significantly increased in T1 group c...
Hao Zhang,1,2,3,* Hai Tao Nie,3 Tie Wei Ma,3 Zi Yu Wang3 and Feng Wang3
... hide, internal organs + blood) and empty bodies of the lambs were weighed, ground, mixed, and subsampled for chemical analyses. The Ca, P, Na, and Mg were mainly distributed in bone, except for K, which was mainly distributed in the muscle tissues. The net macromineral requirements for maintenance were 20.20 mg Ca, 13.50 mg P, 3.80 mg Na, 7.91 mg K, and 1.10 mg Mg/kg empty body weight (EBW) for ewes. The net requirements for growth ranged from 11.79 to 12.06 ...
Ali Haider Saleem1,*, Khalid Javed2, Masroor Ellahi Babar3, Tanveer Hussain3, Asad Ali2, Afzal Ali2, Nisar Ahmad2, Muhammad Zahid Farooq1 and Muhammad Dawood2
... the DNA extraction from blood samples, a 1486 bp fragment of exon three was amplified and sequenced. Sequence analysis revealed single nucleotide polymorphism (T>A at position 483) and a sheterozygous condition (T>W at position 483). Generalized linear model was used to minimize the environmental effects. The variation i.e. “W” type heterozygous condition showed a higher average daily weight gain. Sequence analysis of LEP gene confirm...
Shireen Ihsan Izzaddeen1 and Ali Kaygısız2,*
.../day) on performance and blood parameters of Holstein calves. The study was started with total of 24 calves, randomly categorized into two groups of 12 (6 males and 6 females) however, it was completed with 23 calves because of dying a female at the 8th week of the trial. Addition of Laurel essential oil into whole milk did not cause any significant change in weekly live weight gain (kg), total weaning live weight gain (kg), weekly chest girth measu...
Lili Zhang, Yuanxiao Wang, Yili Kong, Hussain Ahmad, Rui Yan, Li Dong, Jingfei Zhang and Tian Wang*
Ikramullah Khan1,2*, Muhammad Subhan Qureshi2, Sohail Akhtar2, Ijaz Ali3 and Ghufran Ullah4
...rameters determined from blood serum were glucose, cortisol and Heat shock protein-70 (HSP-70). Thermal stress increased all physiological parameterssignificantly (P < 0.001). Holstein Frisian manifested maximumincrease in RT, RR and PR (3.33, 209.50 and 59.41%) than crossbred (0.59, 40.22 and 22.0%), Sahiwal (0.78, 42.54 and 34.31%) and Achai (0.78, 39.22 and 33.85%) respectively (P < 0.001).Thermal stress increased biochemical changes significantly (P ...
Ahmad Farooq1, Usman Waheed2,3, Hasan Abbas Zaheer2,3, Abdul Rauf4, Abida Arshad5, and Muhammad Arshad1,*
... dependent upon lifelong blood transfusion. Multiple transfusions expose them to many blood borne diseases, most commonly hepatitis B and C. The aim of current study was to determine the prevalence of HBV and HCV infections among thalassaemia major patients. The study was conducted from June – December 2016, at the Thalassaemia Centre, Shaheed Zulfiqar Ali Bhutto Medical University, Islamabad and the Pakistan Thalassae...
Naimat Ullah1,3,*, Aneela Zameer Durrani1, Muhammad Avais1, Nisar Ahmad2, Sana Ullah3, Muhammad Shuaib Khan3, Khalid Mehmood5, Mumtaz Ali Khan1 and Ikramul Haq1
...Theileriosis is a common blood protozoan disease of sheep in tropical and subtropical areas. The current study was designed to inspect the prevalence and its correlation with various risk factors and host biomarkers concerned with the occurrence of theileriosis in sheep. Total 600 blood samples were taken each 200 from districts Bannu, Tank and Dera Ismail Khan of southern Khyber Pakhtunkhwa Pakistan and screened through
Muzafar Shah1,*, Mazhar Ali2, Sardar Azhar Mehmood2, Shabir Ahmad2, Khushi Muhammad3, Israr Alam1, Kausar Saeed1
...tive (26.08%) out of 230 blood samples and low prevalence in U.C Ghalaigai with 42 positive (13.12%) out of 320 samples. Gender wise prevalence of malaria disease shows male 106 (27.53%) out of 385 whereas female 98 (16.01%) out of 612.
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Jehangir Khan1,*, Rasheedullah1, Bakht Tarin Khan2, Ayaz Ahmad3, Muhammad Salman4 and Khalid Khan5
...of ICT for screening the blood against HCV. This work will provide the active HCV prevalence data for further research/studies, which can be helpful to health policy makers to plan strategies for the control of hepatitis C disease particularly in district Shangla and generally in Pakistan.
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Hamayun Khan1,*, Abdul Wahab1, Navaid Kazi2, Younas Muhammad1,Summaya Kazi3, Salman Kazi4, Azmatullah Khan1, Ikramullah Khan5 and Shakoor Ahmad1
...eatinine, triglycerides, blood urea nitrogen (BUN), alanine transaminase (ALT) and aspartate transaminase (AST). The current study demonstrated the ascertained value in amniotic fluid for glucose, total protein and albumin at day 18, 25 and 28 was 42.9±0.80, 37.4±0.90, 36.5±0.88mg/dl; 17.9±0.30, 16.1±0.22, 13.9±0.34g/l; 13.9±1.6,11.5±0.75, 8.8±0.15g/I whereas that of creatinine, triglyceride, BUN, ...
Muhammad Nazar Aftab1, Ahmed Ali1, Muhammad Asad1, Sadia Fatima2 and Furhan Iqbal2,*
...period of time. Complete blood count were determined in both experimental treatments at the end of dose supplementation. Our results revealed that oral supplementation of 80mg/Kg body weight of AlCl3 for 16 days did not affect (P > 0.05) any of the studied parameters of complete blood count. On the other hand, serological parameters like triglycerides (P = 0.0075 ), total proteins (P = 0.042) and creatinine (P ...
Muhammad Asim Khan1,*, Shahid Niaz Khan1, Sanaullah Khan2, Abdul Jabbar Khan3, Muhammad Adnan4, Nawab Ali5 and Ijaz Ali6
...%).
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Mumtaz Ali Khan1, Aneela Zameer Durrani1, Sher Bahadar Khan2, Shehla Gul Bokhari1, Ikramul Haq1,*, Imdad Ullah Khan3, Naimat Ullah4, Naimat Ullah Khan4, Kashif Hussain1 and Azmat Ullah Khan2
...lates were quantified on blood agar and confirmed through PCR. Toxins were extracted, quantified, formalized and adjuvanted with aluminium hydroxide gel. Safety, sterility and stability of the toxoid were ascertained. Bacterin toxoid of C. perfringens type D was procured from Veterinary Research Institute Peshawar. The immunogenicity of toxoid vaccines and bacterin toxoid vaccine were studied and compared initially in rabbits and then in goats and sheep...
Muhammad Shahid Saeed1, Adeela Shahid2, Samia Jawed3, Muhammad Akram4, Irshad Hussain Qureshi5
...A total of 2ml of venous blood was taken to determine the serum magnesium levels. All patients were given Salbutamol inhalation and intravenous (I/V) Hydrocortisone. The study group was given 2gm of MgS04 intravenously in 20 minutes in a burette and Placebo group was given placebo (0.9% normal saline). Salbutamol inhalation was given at regular intervals and Spirometry was performed to determine lung functions.
Results: The two gr...
Nasreen Ishaq1, Arif Rasheed Malik2, Zameer Ahmad1, Saad Ehsan Ullah3
...ermination of height and blood groups identification. Among these, DNA analysis and dental data provide easiest identifications, however, these techniques are expensive and not readily available necessitating additional techniques for identification. One of such novel approach is cheiloscopy i.e. study of lip print patterns.
Methodology: In order to investigate the lip prints-based identification, a study was conducted in the Shaikh Kh...
Rana Khurram Aftab1*, Farhat Ijaz2, Samia Jawed3
...pproach towards lowering blood pressure.
Objectives: To determine the frequency of obesity according to body mass index and waist hip ratio and its relationship with hypertension among medical students.
Methodology: It was a descriptive cross sectional study where a total of 213 students of 1st year MBBS and BDS were involved. Their waist circumference, hip circumference, height and weight were recorded. Moreove...
Muhammad Nusrullah*1, Muhammad Younus2 and Yasir Nasir3
...>Abstract | Arterial blood gas analysis is an important test for determining acid base balance of the body. Chronic obstructive pulmonary disease is characterized by chronic airflow limitation which is not fully reversible and it can lead to respiratory failure.
Objective: To determine the correlation between arterial and venous blood gases in patients presenting with chronic obstructive pulmonary dise...
Mumtaz Akhtar, Iffat Naheed, Khadija Waheed* and Amna Khanum
...was observed between the blood loss and postpartum haemorrhageamong the patients (i.e. p-value=0.000). A low frequency of postpartum hemorrh agein women experiencing normal vaginal delivery after misoprostol was administered. The blood loss was recorded to be very low among females.
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