The mRNA Expression of Host Defense Peptides in Chicken Erythrocytes are Highly Related to Tibial Dyschondroplasia and Induced by Recombinant Glutathione-S-Transferase A3 Protein
The mRNA Expression of Host Defense Peptides in Chicken Erythrocytes are Highly Related to Tibial Dyschondroplasia and Induced by Recombinant Glutathione-S-Transferase A3 Protein
Sheng Niu1, Ali-Raza Jahejo1, Fa-jie Jia1, Xin Li1, Guan-bao Ning1, Ding Zhang1, Hai-li Ma1, Wei-fang Hao2, Wen-wei Gao1, Yu-jun Zhao1, Shi-min Gao1, Jian-hui Li1, Gui-lan Li1, Fang Yan1, Rong-kun Gao1, Huan-chun Chen1,3 and Wen-xia Tian1,*
ABSTRACT
Chicken erythrocyte, which is the most abundant cell subset in blood vessel, also exists in cartilage zone. It has recently been shown that chicken erythrocytes constitutively express toll-like receptors (TLRs), cytokines, and avian β-defensins (AvBDs). Host defense peptides (HDPs) are important effector molecules in innate immunity. In this study, thiram has been used to induce tibial dyschondroplasia (TD) and the chickens were injected with different doses of recombinant glutathione-S-transferase A3 (rGSTA3) proteins. We explored the responses of HDPs in erythrocytes to thiram-induced TD and rGSTA3 protein by quantitative real time PCR (qRT-PCR). The results showed many HDPs expressions were suppressed by thiram-induced TD, and the expressions of HDPs were upregulated by rGSTA3 protein. These findings demonstrated that mRNA expressions of HDPs are highly related to thiram-induced TD, and rGSTA3 protein enhanced the immune response of chicken erythrocytes by up-regulating HDPs expression. Future studies may be aimed at elucidating the association between the expression of these peptides and the development of the TD.
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August 2019
Vol. 51, Iss. 4, Pages 1203-1598
