The aim of this research work was to examine the relationship of serum resistin with metabolic markers in obese Pakistani subjects. Anthropometric parameters including age, body mass index (BMI), waist circumference, waist-hip ratio (WHR), diastolic and systolic blood pressure, lipid profile and fasting glucose, serum resistin and insulin of three hundred over weight, obese males and females, 17 to 30 years and 100 comparable control subjects were included. Serum resistin and insulin, fasting glucose, triglycerides and cholesterol were found significantly high in overweight and obese groups as compared to the normal weight group (p<0.01). In overweight group, resistin levels were significantly related with cholesterol, insulin levels and Homeostatic Model Assessment of Insulin Resistance (HOMA-IR) (r = 0.375, p = .000; r = 0.336, p < 0.01; r = 0.301, p<0.01). In obese group, resistin showed a significant positive correlation with BMI, fasting glucose, systolic blood pressure and diastolic blood pressure (r = 0.332, p < 0.01; r = 0.278, p<0.01; r = .279, p<0.01 and r = 0.329, p<0.01), respectively. To conclude this study suggests a positive association between serum resistin and metabolic markers of obesity in young Pakistani subjects.