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Souvik Ghosh, Nobumichi Kobayashi

British Journal of Virology
...etic diversity of the antigenically important RVA VP7- and VP4- protein encoding genes are important for vaccine development or judging the efficacy of existing RVA vaccines, they do not always provide conclusive information on the overall and complex genetic makeup of RVAs, as the remaining 9 RVA gene segments are also susceptible to the forces governing RVA genetic diversity. Whole genomic analysis of RVAs has revolutionized the study of RVA genomics, provid...

El-Sayed M. Abdelwhab, Jutta Veits and Thomas C. Mettenleiter

Avian Influenza H5N1 in Egypt: What we Know and What we have to Know?
...epitopes resulting in antigenic drift and the establishment of infections in vaccinated poultry. The Egyptian H5N1 viruses remain susceptible to oseltamivir, but genetic markers indicating resistance to amantadine have increased dramatically. In this mini-review, we discuss recent findings on the H5N1 viruses endemic in poultry and humans in Egypt, and suggest possible options to control the infection.

...

Hanqin Shen1,3, Boliang Wu1,2, Guangwei Li1, Feng Chen1, Qingbin Luo1,3, Yimin Chen1, Qingmei Xie1,2,3*

...genetic evolution and antigen variation. Moreover, the ratio of L226 in the haemagglutin (HA) receptor binding site has increased, which indicates that the H9N2 virus has the increasing potential to infection and cause human epidemics. The threat to the poultry industry and public health should not be ignored. In this review, we summarised the findings of recent studies on H9N2 AIVs epidemics and strategies for their prevention in poultry in China.

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Qin Zhao1,2, Yani Sun1,2, Gaiping Zhang1,3, Frederik Widen4, En-Min Zhou1,2,*

zhouem@nwsuaf.edu.cn

... immune response. Six antigenic domains in the capsid protein have been identified. The fragmented proteins in these domains are analyzed for their application in serological diagnosis of HEV.

...

James Ayukepi Ayukekbong1,2

E-mail | james.ayukekbong@microbio.gu.se

... malaria are endemic in Nigeria. Two independent studies among febrile patients revealed dengue IgG seroprevalence of 73% and malaria prevalence of 80% in some areas of the country. The diagnosis of the disease is further complicated by the fact that, dengue fever mimics malaria fever and the prevalence of dengue-malaria co-infection is high. Differential diagnosis of fever is therefore necessary to initiate the appropriate clinical care. Despite the endemicit...

John Martin Fischer

...lose, and with the intelligent and patient help of Pereboom, one can see the great appeal of his approach to a whole range of interrelated issues. I’m not going to mince words: this book is a masterful and comprehensive articulation of Derk Pereboom’s very important and original theory of free will and moral responsibility. Throughout his career, and especially here in this book, Pereboom has developed and defended one of the real “contende...

Muhammad Ashraf Khan1, Hizbullah Khan1, Abid Farid2

...ffects of Helicoverpa armigera nu- cleopolyhedrovirus (HaNPV) applied to eggs of Sitotroga cerealella Olive, on emergence of and para- sitism by female Trichogramma chilonis (Ishii) emerged from HaNPV- treated host eggs. The percent emergence (mean) of tiny parasitoid from host eggs treated during egg, larval and pupal stages with the virus at field dose (x), 2x dose, and 0.5x dose ranged from 83.2- 87.3%, 87.1- 91.6%, and 83.7- 89.7%, respectively, relative t...

Kemi Funmilayo Omotesho1*, Isreal Ogunlade1, Opeyemi Eyitayo Ayinde2

... rendered in Oyo State, Nigeria. Structured interview schedule was used in eliciting information from 195 farmers in rural communities across the four agro-ecological zones in the study area using a two-stage random sampling technique. Data used for the study were analysed using descriptive statistics, Likert scale and the regression analysis. The study revealed that farmers perceived the level of accountability of extension to farmers in the study area as poo...

Benjamin Beit-Hallahmi

...als who are highly intelligent, critical, independent, and confident towards the academic world. Contrary to what some might think, it is not getting a Ph.D., which contributes to individual secularity; it is young secular individuals who are highly likely to commit themselves to an academic life.

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Guoyi Li1, Haiyan Tong1, Jiansheng Chen2, Jinliang Wei2, Xiaoming Zhao2, Guoping Zhang2, Zixue Shi2*

E-mail | shizixue@gmail.com

...iew aims to reveal the exigency of developing a worldwide effort to acknowledge the importance of performing an extensive study of this deadly disease.

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Opeyemi Eyitayo Ayinde1*, Kayode Ayinde2, Tofunmi Emmanuel Ilori1, Raphael Olanrewaju Babatunde1

E-mail | opeyemi@unilorin.edu.ng

...jor staple food in Nigeria. Secondary data were sourced from various organizations and used for this study. These data were transformed from their nominal value to real value and analysed using descriptive statistics, unit root test, ordinary least square model, Cochrane-Orcutt, least absolute deviation estimators and simultaneous equation model. The study further established that the prices of staple foods were inter-related. Also, it...

Oladipo Elijah Kolawole*, Oloke Julius Kola, Awoyelu Elukunbi Hilda, Olasunmibo Oluwatobi Gabriel, Ogunyale Tolulope Temitope

E-mail | koladipo2k3@ yahoo.co.uk

Temitope Oluwasegun Cephas Faleye1, Johnson Adekunle Adeniji1, 2 *

...2 lineages described in Nigeria between 2005 and 2011 originated from NPEV-Cs circulating in Northern Nigeria. Here, we further investigate whether NPEV-Cs circulating in south-western Nigeria (SWN) contributed to the emergence of these lineages. In 2013 sewage contaminated water samples were collected from two sites in Lagos, Nigeria. Samples were conce...

Muhammad Imran1*, Muhammad Mobashar2, Muhammad Irfan3, Shazia Hanif4, Sumaira Hanif5 and Muhammad Abubakar6

...y. Overall the apparent digestibility coefficients of dry matter (DM) and organic matter (OM) were non-significant (P>0.05) whereas digestibility of NDF was significantly increased (P<0.05) in treatment MLM4. When compared among treatments, the value of crude protein (CP) digestibility coefficient was high (P<0.05) in treatment MLM4 as compared to MLM1. Similar trend was also obse...

Tauseef Taj Kiani1*, Mozammil Hussain1 and Hidayat ur Rahman2

 

...reeding depression in indigenous maize germplasm comprising inbred lines NCMLQ1, NCMLQ2, NCMLD1, NCMLD2, NCMLD3, and NCMLD4. ese genotypes were crossed to get ten F1 hybrid combinations, which were used to develop three generations F2, BC1 and BC2. Heterosis (in F1) and inbreeding depres- sion (in F2, BC1 and BC2<...

Abiodun D. Olabode

...>Rice, a staple food in Nigeria is experiencing decrease in yield over the years. This study evaluates the productive capacity of some selected rice farms in Kwara State, Nigeria, with a view to identify farm management practices that enhance sustainable production of rice in the study area. Primary data were obtained through the use interview schedule. Eighty copies of structured interview were purposively employed to obtai...

Mohammed A. Rohaim1*, Rania F. El Naggar2, Ahmed M. Helal3, Hussein Ahmed Hussein1 and Neil LeBlanc4

...haracterizations of the pigeon paramyxovirus type I (PPMV-1) from pigeon in Egypt during 2014. Pathotyping, based on the mean death time (86 hours) in eggs and the intracerebral pathogenicity index (1.2), and the amino acid motif, (112KRQKR116), at the cleavage site of the fusion (F) protein indicated the emergence of PPMV-1 in Egypt. Phylogenetic analysis of the F and haemagglutinin-neuraminidase (HN) genes indicated that t...

Amitha Reena Gomes1, Belamaranahally Muniveerappa Veeregowda2, Sonnahallipura Munivenkatappa Byregowda1, Vinayagamurthy Balamurugan3*

...nes and/or diagnostic antigens. The recombinant viral proteins, virus like particle based vaccines, bivalent/multivalent vaccines, recombinant viral vectored vaccines, RNA interference as a therapy, suicidal DNAs, synthetic epitopes and peptides, reverse genetics, anti idiotypic antibody based vaccines and helper cell dependent diagnostics represent the present vaccine/diagnostic development strategies for the effective control and eradication of PPR. This rev...

Neil Levy

... growth in machine intelligence, resulting in the appearance of AIs that are vastly superior to us cognitively – will or might occur in the relatively near future. Understandably, many are worried by this prospect. In this paper, I argue that we have less to fear from the singularity than many people think. Depending on how we define ‘singularity’, it is either less likely to occur, or it will likely occur in a form that is not threatening to...

Neil Levy

... growth in machine intelligence, resulting in the appearance of AIs that are vastly superior to us cognitively – will or might occur in the relatively near future. Understandably, many are worried by this prospect. In this paper, I argue that we have less to fear from the singularity than many people think. Depending on how we define ‘singularity’, it is either less likely to occur, or it will likely occur in a form that is not threatening to...

Muhammad Abubakar1*, Shumaila Manzoor2, Jonas Johansson Wensman3, Emeli Torsson3, Qurban Ali1 and Muhammad Munir4

...tion was confirmed by antigen capture ELISA and RT-PCR in both swab and body tissue samples. The molecular characterization revealed clustering of the PPRV within lineage IV with significant substitutions in the nucleoprotein (NP) gene. Genetic variations within NP gene, and possibly in other proteins which are essentially mediating protective immunity, may explain the extreme infectious nature of the virus and its host-specific pathogenesis. Moreover, underst...

Christopher U Orji1*, Ignatius O Onyeocha2, Steven S Shaida3, Peter M Dede4, Bitrus Yakubu5, Elijah E Ella6 and Pam D Luka5

Koksal Karadas1, Muhammad Tariq2, Mohammad Masood Tariq3* and Ecevit Eyduran4

...milk yield amounts of indigenous Akkaraman ewes reared in Sanliurfa (137 farms) and Hakkari (113 farms) provinces of Turkey. For the determination process, the statistical predictive accuracy of GLM (General Linear Model), CART (Classification and Regression Tree), CHAID (Chi-square Automatic Interaction Detector), Exhaustive CHAID, and a ANN type MLP (Multilayer Perceptron) in the prediction of lactation milk yield in sheep was measured comparatively by model...

Paul I. Ankeli, Mashood A. Raji, Haruna M. Kazeem, Moses O. Odugbo, Nendir J. Umaru, Idowu O. Fagbamila, Livinus T. Ikpa, Obinna O. Nwankiti, Issa A. Muraina, Pam D. Luka and Nicholas D. Nwankpa

...attle in Plateau State, Nigeria. One hundred and sixty six ear swab samples (n=166) were cultured from which eight (8) Mycoplasma species were isolated and characterized using conventional biochemical tests and polymerase chain reaction (PCR), respectively. Six (6) were observed to ferment glucose, reduce tetrazolium chloride and hydrolysed casein. They had no phosphatase activity, did not produce ‘film and spots’ and neither hydrolysed arginine no...

Gerald E. Marsh

...e universe, and of intelligence in particular, appears to have no meaning. This essay explores the epistemological basis of this lament.

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Halide Nihal Açikgöz1*, Serdal Kenan Köse2 and Ali Açikgöz3

 

 

...us), Lucilia sericata (Meigen) and Lucilia richardsi (Collin) (Diptera: Calliphoridae) were identified. C. vicina was found in whole year in 2012 and 2015. All of the fly species identified have forensic importance. The highest taxa richness was observed in 2012, in May (307; 73.80%), while the lowest was in March (23; 5.53%). The highest taxa richness was observed in June (544; 21.92%), while the lowest was in January (6; 0.24%) in 2015. Macro and micro clima...

Syeda Farzana Bibi*, Lal Badshah and Siraj-ud-Din

...tic in future for the indigenous flora.

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Adedeji A.J, Sati N.M, Pewan S.B, Ogbu K.I, Adole J.A, Lazarus D.D, Ijiwo S.J, Okpanachi A, Nwagbo I.O, Joannis, T.M and Abdu P.A

... in Jos, Plateau State, Nigeria with mortality of up to 79.12%. The clinical signs observed were prostration, ruffled feathers, diarrhea, anorexia, trembling and mortality that lasted for 6 days. Postmortem findings include ecchymotic hemorrhages in the breast and thigh muscles, mucosa of the junction between the proventriculus and gizzard, severely enlarged and hemorrhagic bursa of Fabricius (BF) and splenomegaly. The bursa of Fabricius was harvested for labo...

Ecevit Eyduran1, Daniel Zaborski2*, Abdul Waheed3, Senol Celik4, Koksal Karadas5 and Wilhelm Grzesiak2

...y measurements in the indigenous Beetal goat of Pakistan. Moreover, the results obtained with the data mining algorithms were compared with multiple linear regression (MR). A total of 205 BW records including one categorical (sex) and six continuous (head girth above eyes, neck length, diagonal body length, belly sprung, shank circumference and rump height) predictors were utilized. The Pearson correlation coefficient between the actual and predicted BW (r) an...

Ali Raza Awan*, Sehrish Firyal, Muhammad Tayyab, Lala Rukh, M. Zia ul Haq, Shagufta Saeed and Muhammad Wasim

...tify and classify the indigenous wild Rose-ringed Parakeet of Pakistan using Cytochrome b (Cytb) gene polymorphism. Mitochondrial DNA of 24 unrelated Pakistani wild Ring-rose Parakeets was isolated and utilized for amplification and DNA sequencing of Cytb gene. The Phylogenetic analysis of the Cytb gene indicated that the Pakistani wild Rose ringed Parakeet was mono-phyletically claded with P. k. manillensis with a sequence similarity of 99.37%. Comparative an...

Süleyman Kök1*, Sertaç Atalay1, Hasan Semih Eken2 and Mustafa Savaşçi3

...e genotype profile of indigenous types for TGC breed. In order to characterize TGC population, two polymorphic loci in cattle Calpain gene (CAPN1) and one polymorphic locus in Calpastatin gene CAST were studied. In order to sort the allele variants (G/C), PCR products, the ones in CAST with RsaI enzyme, was cut using RFLP method. The allele variants (G/C) of CAPN1 316 and (C/T) of CAPN1 4751 were determined with ARMS-PCR method. This study was conducted to det...

Tayiba Latif Gulraiz1, Arshad Javid1*, Syed Makhdoom Hussain2, Muhammad Shahbaz3, Irfan3 and Sharoon Daud4

...) and six bacterial (Acaligenes, Azotobacter, Bartonella, Nitrsomonas, Pseudomonas and Salmonella) genera were isolated from guano while five fungal (Alternaria, Aspergillus, Chrysosporium, Exophilaand Scopulariopsis) and four bacterial (Bacillus, Corynebacterium, Listeria and Streptomycete) genera were common in bolus and guano samples. Seasonal variations were recorded in occurrence of various fungal and bacterial genera. From bolus samples, two fungal Asper...

Muhammad Raheel1,*, Nazir Javed2, Sajid Aleem Khan2, Hafiz Muhammad Aatif3 and Sohail Ahmed4

Oladipo Elijah Kolawole, Oloke Julius Kola and Awoyelu Hilda Elukunbi

...in Ogbomoso, Oyo State, Nigeria. Two hundred and seventy eight HIV-infected individuals were screened for EBV antibodies over a period of four months (August to November, 2014). The sera of individuals were subjected to serological assay for IgM using enzyme-linked immunosorbent assay. The mean age of subjects was 39.5±0.48 years and the mean CD4+ was 384.27 ±17.77 cells/µl. Out of 278 subjects tested, 11 (4%) were positive for anti-EBV IgM...

G.E. Odo1*, E.J. Agwu1, N.I. Ossai2, C.O. Ezea3, J. Madu1 and V. Eneje2

...ure for pest control in Nigeria. This study investigated the effects of aluminium phosphide on the behaviour, haematology, oxidative stress biomarkers and biochemistry of African catfish (C. gariepinus) juvenile on days 7, 14, 21 and 28. Fish were exposed to 0.175, 0.0875 and 0.035 mg/L corresponding to 1/10, 1/20 and 1/50 of the 96 h LC50 value (1.75) of the pesticide. Behavioral responses which include colouration of the fish skin and opercula colouration we...

Nighat Sultana1,2,*, Shazia Iftikhar1, Nafeesa Qudsia Hanif2 and Iffat Tahira2

...of Punjab, Pakistan. A. niger, A. flavus and A. fumigatus were the most dominant followed by A. terrus and A. ochraceous in fresh and ensiled maize fodder. Total fungal counts ranged from 1×103 to 4×103cfu/ml for all species in both fresh and ensiled maize fodder. Total aflatoxins (AFB1, AFB2, AFG1 and AFG2) and ochratoxin A (OTA) analysis showed that AFB1was present with high frequency in fresh (37.5%) and ensiled (41.66%) fodder, with an average ...

Fukuan Du1,2, Yan Li2, Zhixin Wen1, Ruguo Chen1 and Pao Xu2*

...nized, of which 10,310 unigene sequences contain more than one SSR. These genetic markers are ideal molecular markers for the following investigating genetic diversity, constructing genetic maps, and marker assisted selection.

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Oluwasogo David Olorunfemi, Oluwasegun Adetokunbo Adekunle, Felix Olayinka Oladipo, Temitope Oluwaseun Oladele and Oladimeji Idowu Oladele

...ra State, North Central Nigeria. Data were collected using a two-stage sampling technique to select one hundred and sixty respondents for the study. A purposive selection of two Local Government Areas each from the two Agricultural Development Programme administrative zones (Zones C and D) in Kwara State where fish farming is prominent and well-practiced was carried out. Forty fish farmers were then randomly selected from the fish farmers’ association ch...

Aziz-ul-Rahman, Farooq Yousaf, Naveed Anwar and Muhammad Abubakar

... performed using PPRV antigen to determine antibody titres level. The overall prevalence of antibodies to PPRV in goats and sheep were 66.22% (521 samples) and 65.76% (444 samples), respectively. Sero-prevalence of anti-PPRV antibodies was found higher in adults (69.14% with GMT 17.91) than in young (62.47% with GMT 12.85). Furthermore, seasonal seropositivity seasons was recorded and sero-prevalence during summer was found to be higher (72.84% with GMT 20.18)...

Mariano Gastón Asla

... life and signs of intelligence in the outer space. My goal in this article is twofold. First, in a practical level, I will explore some arguments in favor of the scientific interest regarding the search of extraterrestrial life. Second, from a theoretical perspective, I will present some examples that show the philosophical value of the extraterrestrial intelligence fiction as a mental experiment of remarkable heuristic fer...

Muhammad Shahzad Ahmed and Dilnawaz Ahmed Gardezi

...es with PCA analysis at eigenvalue >1 and grouped 20 genotypes into five clusters at Euclidean distance (ED) 7. Cluster analysis revealed that the members of clusters I, cluster III and Cluster V diverse genotypes that should be utilised to initiate breeding program. The genotype HSF-242 was the outlier at ED 8. Out of twenty genotypes tested only five (S-03-US-694, S-05-FSD-307, S-05-FSD-317, S-08-FSD-23 and S-08-FSD-19) are responded flowering and viable ...
Eman Mahmoud El-Diasty1,Madeha Abd El-Halim Ibrahimand Ghada Kamal El Khalafawy2,*
... bp. PCR amplicons were digested with enzyme MspI and the generated bans corresponded to the predicted size.
...
Lingtong Ye1, Chao Cao1,2, Bin Tang1,2, Tuo Yao1, Ruixuan Wang1 and Jiangyong Wang1*
...the major spines on chaetiger 5, and the shape of the pygidium. The COI gene sequence demonstrated that the intraspecific distance of P. websteri was 0.33%, whereas the interspecific distance of P. websteri ranged from 18.88% (with P. brevipalpa) to 24.79% (with Boccardia proboscidea). The intraspecific genetic distances of polydorids examined in the present study ranged from 0.33% to 1.67%, whereas the interspecific distances range...
Rashid Mahmood1*, Saima Asad2, Ghulam Sarwar1, Waqar Ahmad1, Ziyad Abdul Qadir1, Ammara Balouch1 and Muhammad Khalid Rafique1
...ht containers in the refrigerator. Our results showed maximum foraging ratio (59.88 ± 2.89) and pollen collection (145.8±8.79) during 1000-1200 hours followed by the duration of 1200-1400 hours when foraging ratio was 58.24 ± 3.03 and pollen collection was 134.4±5.49 and during 0800 – 1000 hours when foraging ratio was 48.36 ± 2.84 and pollen collection was 121.8±6.73, whereas, the lowest values were found during...

Gabriela Mansano do Nascimento, Helena Lage Ferreira and Clarice Weis Arns

 

...is independent of the antigen and depends on the ability of the host to recognize pathogens by means of specific pattern recognition receptors. This interaction induces the production of cytokines, chemokines and interferons. Through Janus kinase/signal transducers and transcription activators pathway, interferon induces the transcription of interferon-stimulated genes (ISGs), responsible for playing an essential and decisive role in the virus pathology. Some ...

Pam D Luka, Frank N Mwiine, Bitrus Yakubu, Joseph Erume, Ricardo Pérez-Sánchez, Hermann Unger and David Shamaki

... first report of ASF in Nigeria, periodic outbreaks have continued to occur among domestic pigs with a few incidences in wild pigs. However, to investigate the risk of a tick-domestic pig transmission cycle in Nigeria, 3,288 serum samples were collected from domestic pigs from 10 states and analysed for anti-tick antibodies using the recombinant TSGP1 indirect ELISA. Of the samples analysed, 13.4% (442/3,288) showed moderate...
Rashid Mahmood1,*, Waqar Ahmad1, Muhamamd Khalid Rafique1, Ghulam Sarwar1 and Anjum Shahzad2
...whereas population of indigenous Apis cerana varied between 3.80 to 5.44 h per 250 flowers in 2013 as compared to year 2014 where it was recorded as 4.34 to 5.56 honey bees per 250 flowers. Apple fruits yield per tree ranged between 214.56 to 218.64 in 2013 and 2014 respectively. Average fruit weight varied between 124.84 to 127.34 g, average number of seeds varied between 7.95 to 7.73 seeds per fruit and yield per tree was recorded between 28.92 to 31....

Idowu Fagbamila1*, Adaobi Okeke2, Micheal Dashen2, Patricia Lar2, Sati Ngulukun1, Benshak Audu1, David Ehizibolo1, Paul Ankeli1, Pam Luka1, Maryam Muhammad1

...veloping countries like Nigeria where access to portable water is lacking, it is not uncommon to “reuse” rinse water for processing several batches of poultry carcass.Several samples were collected from three poultry slaughterhouses within Jos metropolis at intervals for a period of 6 months to check for presence of Salmonella during various stages of processing. Salmonella was isolated from at least one of the four matrices in the three LBM with a...
Shahzad Ali1,*, Heinrich Neubauer2, Falk Melzer2, Iahtasham Khan1, Shamim Akhter3,Tariq Jamil2 and Sajid Umar3
...ection as compared to indigenous cattle. Routine screening and strict biosecurity measures are recommended for prevention of infection in animals. Humans must consume milk after pasteurization. 
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John G. Bruno

...emulating rickettsial antigens to enable sensitive detection of rickettsia exposure in humans who have seroconverted. Such anti-idiotypic aptamer antigens might also serve as rickettsial vaccines, if fully developed. The top five highest affinity R. rickettsii and top five R. typhi anti-Fab aptamers as assessed by enzyme-linked aptamer sorbent assay (ELASA) screening were shown to specifically bind their cognate antibodies w...
Adel Eid Mohamed Mahmoud*
...ntrate feed mixtures on digestibility, blood and ruminal parameters, growth performance, meat analysis and feed intake of growing lambs. Fifty one Barki lambs with body weight of 30±0.57 kg; in three groups (17 animals, each). Experimental rations were composed of concentrate and clover hay in a 60:40 concentrate: roughage ratio.There was no effect of bakery by-products inclusion on nutrients digestibility and nutriti...
YaQiu Liu and ZhiJian Wang*
...es.Intestinal tract has digestion and respiration functions. Anterior intestine performs digestion/absorption. Posterior intestine performs gas exchange/accessory respiration. In middle intestine, digestion and respiration overlap.
...

Zia Ur Rahman1*, Syed Mehar Ali Shah1, Hidayat Ur Rahman1, Fida Mohammad1, Mian Ahmad Raza1 and Ijaz Ahmad Khan2

...ance potential of 142 indigenous accessions along with two check cultivars against bacterial leaf blight disease at the University of Agriculture, Peshawar-Pakistan during 2011 rice crop growing season. On artificial inoculation with Xanthomonas oryzae pv. Oryzae, the fundamental agent of bacterial leaf blight, most of the studied rice accessions and the two commercial rice cultivars displayed susceptible reactions. Seven rice accessions viz. UoA-2, UoA-5, UoA...

Neerja Agrawal, Mukesh Srivastava, Akhilesh Tripathi and Amrendra Singh

Survey and monitoring of pests, parasites and predators of pulse crops in central and eastern Uttar Pradesh
... conducted in chickpea, pigeonpea and lentil crops in different districts of Uttar Pradesh.Atotal of 22 districts were surveyed to record gram pod borer Helicoverpa armigera, Bihar hairy caterpillar, Spilarctia obliqua, plant hopper of Pentatomorpha group, termite Odontotermes spp, and cut worm Agrotis ipsilon in chickpea whereas Campoletis chlorlidae was recorded as natural enemy feeding on H. armig...

K.N. Ahmed, S.H.A. Pramanik, M. Khatun A. Nargis M. R. Hasan

Efficacy of plant extracts in the suppression of insect pests and their effect on the yield of sunflower crop under different climatic conditions
...Phyticidae),Heliothis armigera (Hb.) (Lepidoptera: Noctuidae), Monolepta signata(Oliv.) (Coleoptera: Chrysomelidae) and Epilachna septima Dieke. (Coleoptera: Coccinellidae) . The efficacy of five kinds of plant extracts or botanicals viz., Neem (Azadirachta indica) seed oil, castor ( Ricinus communis) oil, a mixture of Neem seed oil and sesame oil, leaf extracts of custard apple (Annona squamosa ) and Bara Bishkatali ( Polygonum orienta) were tested against th...

1Bikash Ranjan Ray and 2Mrinal Kanti Dasgupta

Management of root holoparasite Aeginetia pedunculata of (Orobanchaceae), causing wilt of sugarcane by trap and catch crops
...orchorus olitorius L.), pigeon pea (Cajanus cajan (L.) Millsp.), and groundnut (Arachis hypogaea L.) were found effective as trap crops for their ability to germinate the seed of A. pedunculata. Sorghum (Sorghum bicolor (L.) Moench) was identified as a catch crop for its ability to support further growth and development of A. pedunculata up to flowering.

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Nilesh Suresh Gole and Bijan Kumar Das

Biology of Dichromia sagitta (Fabricius) (Noctuidae: Lepidoptera), a serious pest of Indian ipecac, Tylophora indica
...amily Asclepiadaceae, indigenous to India. In West Bengal, we observed the herb to be heavily infested by a defoliator pest, Dichromia sagitta (Fabricius) (Noctuidae: Lepidoptera). A study was conducted in the field and laboratory of All India Coordinated Research Project on Medicinal & Aromatic Plants and Betelvine (AICRP on MAPB) at Bidhan Chandra Krishi Viswavidyalaya (BCKV), Kalyani, Nadia, West Bengal during 2010-2011 on seasonality, extent of damage ...

A.A. Oyerinde, P.Z. Chuwang, P.Z. and G.T. Oyerinde

Evaluation of the effects of climate change on increased in-cidence of cowpea pests in Nigeria
...hing and Research Farm (Nigeria) in order to determine the impacts of climate change on the population of insects associated with cowpea production in the region. The result showed an increase in the number of taxa encountered on the cowpea field from 21 to 31 orders of insect in the 2010 and 2011 cropping seasons, respectively. Also, no significant difference was established in the forms of destructions recorded at the various growth stages of the cowpea plan...

Rashid Pervez, Santhosh J. Eapen, S. Devasahayam and M. Dinsha

Characterization of entomopathogenic nematode, Stein-ernema carpocapsae from ginger (Zingiber officinale Rosc.) rhizosphere in India
...here from India. This indigenous isolate could be investigated further for managing insect pests of ginger.

...

K. K. Hegde and B. S. Nandihalli 

Bioefficacy of some indigenous products in the management of okra fruit borers
...e bioefficacy of some indigenous products against okra fruit borers, Earias vittella Fabricius and Helicoverpa armigera Hubner. Different indigenous products like NSKE (5%), GCKE, sweet flag extract (5%), garlic extract (5%), cow dung (10%) and cow urine (10%) were tried individually and in combinations along with endosulfan (0.07%) and untreated control. Repeated sprays of GCKE recorded l...

Shekharappa

Biological control of earhead caterpillar, Helicoverpea armigera Hubner in sorghum
...nd NPV of Helicoverpa armigera (HNPV) and compared with chemical control with Melathian 5% @ 30 kg.ha-1 for the management of ear head caterpillar (H. armigera) in sorghum ecosystem of University of Agricultural Sciences, Dharwad. The mycoinsecticide N. rileyi was highly infective at both the dosages 1x108 conidia per litre & 2x108 conidia per litre against H. armigera as compared to H...

S. Pal and I. Sarkar

Pests infesting ornamental plants in hilly region of West Bengal
...od borer (Helicoverpa armigera) was found most important inflicting serious damage to Carnation, Gerbera, Gladiolus, Chrysanthemum and Straw flower. The green semilooper, Plusia orichalcea caused damage to Gerbera and Gladiolus by feeding on the leaves. The tobacco caterpillar (Spodoptera litura) was serious on Gladiolus and Anthurium. Cutworm (Agrotis segetum) damaged the seedlings of Gladiolus. Among the Coleopteran pests, the Blister beetle (Mylabris p) was...
Burhan Azam1, Muhammad Naeem Tahir2,*, Faisal Shahzad2, Abdul Ghaffar3, Ghulam Abbas4, Madiha Gohar5 and Saima1
...rtant role by enhancing digestibility of fibre. This study was carried out to evaluate the effects of varying level of fibrolytic enzyme in concentrate ration on dry matter (DM) intake, milk production and composition, and diet digestibility in Nili Ravi buffaloes. Four lactating Nili-Ravi buffaloes of 3rd and 4th parity and milk production of 8±2.3 kg/day were selected and offered a basal diet b...

Gadeeyya G and Ratna Kumar P.K.

..., Crotalaria verrucosa, Digera muricata, Sida cordifolia, Ipomoea pestigridis and Trianthema portulacastrum were selected for in vitro studies. Fungal isolates namely Ascochyta cypericola, Bipolaris sp., Chaetomium globosum, Colletotrichum capsici, Colletotrichum sp., Curvularia lunata, Curvularia tuberculata, Fusarium oxysporum, Helminthosporium sp. and Gibbago trianthemae were isolated by inoculation of parasitiz...

Mahmoud Said1*, Abd El Satar Arafa1, Mohammed A. Rohaim2* and Hussein A. Hussein2

...day of age using both antigens (RE-5 antigen) and (S75/Egy/2015 antigen). Generally, GMT post-challenge was increased as the chicks overcome the infections. In conclusion, our results indicate that vaccination at 5th day old with baculovirus H5+ND inactivated vaccine can interfere with MDA of H5 vaccine and has negative impact on H5 vaccine titer. However, NDV titer remained unaffected und...
Ahmad Ali1,*, Sheikh Muhammad Azam2, Khalid Javed Iqbal1, Ghulam Mustafa1 and Mujahid Kaleem3
...rtality of Royal Bengal Tiger (Panthera tigris tigris Linnaeus 1758). There are five big cat species in the genus Panthera and tiger is the biggest one. Data were collected by recording observations on breeding of Royal Bengal Tiger in captivity at Bahawalpur Zoo from June 2012 to July 2014 (two years) on prescribe sheets. Data from June, 2003 to December, 2011 (nine yea...
Sidra Manzoor1,Asif Nadeem1,*, Masroor Ellahi Babar2, Wasim Shehzad1, Abu Saeed Hashmi1, Muhammad Imran1, Tanveer Hussain2, Abdul Wajid1 and Maryam Javed1
...ue to environmental or epigenetic effect, sampling number or a breed difference. To know the functional consequences of the identified polymorphism, RNA secondary structure analysis was performed and SNP was found changing the termination loop shape in mRNA. However, to increase the selection efficacy of dairy animals, the effects of candidate polymorphism should further be screened in different environments and genetic backgrounds.
...
Ayesha Ilyas1, Hafiz Azhar Ali Khan2,* and Abdul Qadir1,*
... leaf extracts of six indigenous plants viz., amaltas (Cassia fistula), datura (Datura alba), neem (Azadirachta indica), niazboo (Ocimum basilicum), yellow kaner (Thevetia peruviana) and safeda (Eucalyptus camaldulensis) against the peach fruit fly, Bactrocera zonata (Saunders), at 2% concentration in a free choice bioassays. Acetone, chloroform, petroleum ether and ethanol were used for extraction from l...
Madiha Hashmi1, Abid Hussain1, Shafiq ur Rehman1, Farida Ahmed2, Shahbaz Aslam3,Nadeem Afzal4 and Zaigham Abbas1,*
...abad. Human Leukocyte Antigens (HLA) genes are located on the short arm of the chromosome number 6 and position 21 and the allele frequency is associated with allergy. The study was conducted to investigate the relationship between the aero-allergens and the frequency of HLA-DRB1*11 and HLA-DRB1*12 allele in aeroallergen sensitized individuals. Blood samples of aeroallergen sensitized individuals were collected at the Allergy Centre, NIH, ...

Wesam Hasan Mohamed Mady1*, Bing Liu2, Dong Huang2, Abdel Satar Arafa1, Mohamed Khalifa Hassan1, Mona Mehrez Aly1, Pucheng Chen2, Yongping Jiang2* and Hualan Chen2

...une responses against antigens encoded by recombinant DNA. The goal of this study was to construct Egy-H5 plasmid DNA-based vaccine against a currently circulating avian influenza virus H5N1 strain in Egypt targeting the HA gene. The HA gene of AIV Egyptian strain H5N1 was codon optimized for chicken biased codon and sub-cloned into pCAGGS mammalian expression vector under the control of chicken β-actin promoter. The construct was transfected into 293T HE...
Syed Makhdoom Hussain1,*, Tasneem Hameed1, Muhammad Afzal2, Arshad Javid3, Nosheen Aslam4, Syed Zakir Hussain Shah6, Majid Hussain5, Muhammad Zubair ul Hassan Arsalan1 and Muhammad Mudassar Shahzad1
...erformance and nutrient digestibilityof Cirrhinus mrigala fingerlings fed sunflower meal based diet. The chromic oxide was added as inert marker in the diets. Three replicate groups of 15 fish (Average weight 5.05±0.013 g fish-1) were fed at the rate of 5% of live wet weigh and feces were collected twice daily. The results of present study showed improved growth performance of Cirrhinus mrigala fingerlings in response to phytase...

Raza Ullah1*, Qaisar Shah Safi2, Ghaffar Ali3 and Irfan Ullah

... skills and market intelligence of the citrus growers should be developed to enable them practice self-marketing whereby they can easily pocket the market share of the pre-harvest contractors by eliminating him from the marketing chain. 

...
Kulsoom Akhter1, Tahseen Ghous1,2,Saiqa Andleeb3,*, Faiz-ul-Hassan Nasim4, Samina Ejaz5, Zain-ul-Abdin1, Bilal Ahmed Khan1 and Muhammed Naeem Ahmed1
...racterization of some indigenous heavy metal resistant bacteria isolated from rhizosphere of Tagetes minuta, growing in soil adjoining automobile workshops to investigate their bioaccumulation capacity for three selected metals Cr(VI), Ni(II) and Cd(II). The plant was selected due to its natural abundance in the climate of Kashmir and also in the area of study. On the basis of morphological, biochemical, 16S rRNA gene sequencing, the isolates were ident...
Asim Gulzar1,*, Ather Maqsood1, Munir Ahmed1, Muhammad Tariq1, Muhammad Ali2 and Rahmatullah Qureshi3
...lworm, Helicoverpa armigera (Hubner)is very important polyphagous insect pest with wide host range known for its economic losses worldwide. Its ability to develop resistance to insecticides faster to identify alternates like biocontrol agents, plant extracts etc. In the present study the extract of Citrullus colocynthis in different solvents were evaluated on second instar larvae of H. armigera un...

Lucas Pedroso Colvero1, Paulo Eduardo Brandão2*, Cesar Alejandro Rodriguez Rosales2, Hanny Swam3 and Laura Yaneth Buitrago Villarreal1 

 

...oked when compared to antigenic properties. This study was designed to assess the effect of two different commercial inactivated water-in-oil multivalent vaccines; vaccine A contains IBV (Massachusetts M41 and D274 strains), NDV (Clone 30), aMPV (BUT 8544) and IBDV (classic strain) whereas vaccine B contains IBV (Massachusetts M41 and a Brazil type strain), NDV (LaSota strain), aMPV (TRT strain) and IBDV (GP82 strain). Viscosity values for vaccines A and B whe...
Aqsa Mehboob1, Noor Khan1,*, Usman Atiq1, Khalid Javed Iqbal2, Rafia Tayyab1, Syeda Suhaira Batool1, Hafiza Saleha Batool1, Sana Amjad1 and Mehwish Tanveer1 
...remained same. Apparent digestibility of crude protein and fat was found non-significantly different among treatments. It is concluded that addition of fenugreek in fish feed can improve growth performance and also protein content of the fish meat as well.
...

Sanjukta Chakrabarti1, Colin J. Barrow2, Rupinder K. Kanwar3, Venkata Ramana1*, Rakesh N. Veedu4,5 and Jagat R. Kanwar3* 

...e compared the anti-tumorigenic properties of these three molecules for tumor volume reduction in xenotransplanted SCID mice and by histopathological and immunohistochemical analyses. Our results demonstrated that fusion protein VEGFR1(D1-D3)-Fc efficiently inhibited
the lung cancer growth in vivo. Although the aptamer RNV66 was not found to be as efficient in this study, the results were also not surprising as the aptamer was administered naked and at ...
Naheed Bano* and Muhammad Afzal
... calcium and phosphorus digestibility and different body contents of L. rohita. Phytase and citric acid increased the digestibility of calcium and phosphorus with improvement in body dry matter, protein, fat, energy and ash contents of L. rohita. Acidification and phytase supplementation showed significant (P < 0.05) interaction on body composition and mineral utilization in rohu. Citric acid and phytase sup...

Akerele Dare*, Ashaolu Olumuyiwa Fowowe, Sanusi Rahman Akintayo and Egbetade Adedolapo 

...icultural subsectors in Nigeria. Data on the value of output of crop, livestock and fishery subsectors of agriculture,and the amounts of the fund allocated to each subsector from 1982 to 2013 were subjected to econometrics (time series) analysis. Results established positive and statistically significant influence of the amount of fund apportioned to crop (p<0.01) and livestock (p<0.05) subsectors on output growth in the respective subsectors. Likewise, ...
Liana Mihaela Fericean1* and Mihaela Corneanu2
...ee generations of fundatrigenous. In July, the winged form migrated to the secondary host, where successive generations have been born through parthenogenetic reproduction. Gynopara in October return to the primary host and give birth to wingless oviparae that mate with males and lay winter eggs. The biometrical measurements of Aphis nasturtii help to complete knowledge on external morphology of this species. By knowledge of biology and ecology of Ap...
Teresa Napiórkowska* and Julita Templin
...of a larva of the Eratigena atrica spider, which had a well-developed prosoma and an additional, partly developed head with two segmented pedipalps and one chelicera with two fangs. The larva was obtained as a result of a teratological experiment based on the application of temperatures of 14 and 32°C changed every 12 h for the first ten days of spider embryogenesis.
...
Jing Xin Mao1,2, Guo Wei Wang2, Yuan She Huang3, Rui Wang2, Guo Ze Wang4, Bing Zeng1 and Fu Yuan Zuo1,*
...al related gene among indigenous breed (Rongchang pig) and artificially cultivated breed (Landrace), The Rongchang pig (0 day old, 28 day old, 120 day old) and Landrace BPI (0 day old, 28 day old, 120 day old) gene were expressed in normal liver, heart, lung, kidney, spleen and small intestine. The results showed that: The expression level of BPI in lung of Rongchang pig was relatively higher level compared to that in heart, liver, kidney, spleen and small int...
Hailong Dong1, Hui Zhang2, Kun Li2, Khalid Mehmood2,3, Mujeeb Ur Rehman2, Fazul Nabi2, Yajing Wang2, Zhenyu Chang1,2, Qingxia Wu1,* and Jiakui Li1,2,*

 

...ompA). Moreover O-antigen serotype was tested by slide agglutination test and a mouse model was built to assess the lethality of the E.coli isolates through subcutaneous-infection. Out of 81 E.coli isolates, the most prevalent gene detected was ompA (90.12%), followed by ETT2 (69.14%), irp2 (54.32%), EAST1 (46.91%), CS31A (41.98%), estB (19.75%), eaeA (14.81%), estA (12.35%) and K88

Imran Ali Rajput1*, Tajwer Sultana Syed1, Ghulam Hussain Abro1, Imran Khatri1 and Abdul Mubeen Lodhi2 

...ld. Nevertheless, the indigenous botanical extracts have been used effectively against various sucking pests of cotton but only few studies have been attempted to observe their action against bollworms. Therefore, the present study has been designed to observe the dual action of transgenic cotton and botanical extracts against pink bollworm during two consecutive growing years (2015 and 2016). The three local extracts such as tobacco (Nicotiana tabac...
Ajmal Khan Kassi1,*, Humayun Javed1 and Tariq Mukhtar2
...borer, Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae), is one of the most economically important and widespread pests of okra and causes worldwide yield losses. The pest is mainly controlled by chemicals which cause deleterious effects to human, livestock and environment. To dispense with the detrimental consequences of chemicals, use of resistant cultivars can prove one of the effective and reliable alternative approaches for the managemen...
Aqsa Imtiaz and Abdul Rehman*
...to improve feather meal digestibility in animal feed as an additive.
...
Tariq Mahmood*1, Syed Wasif Ahmed Shah1, Muhammad Rais1, Hira Fatima1, Faraz Akrim1 and Muhammad Sajid Nadeem2
...>Vanellus vanellus, pigeon Columba livia, parrot Psittacula sp.and kingfisher Halcyon sp.did not show any evidence of nesting or breeding. 
...

Muhammad Mudassar Shahzad1*, Syed Makhdoom Hussain1, Farhat Jabeen1, Abdullah Ijaz Hussain 2,Sajjad Ahmad3, Abida Ashraf4,Muhammad Zubair-ul-Hassan Arsalan1

Faiza Hassan1, Mubshara Saadia1,*, Muhammad Sher1, Mian Anjum Murtaza2, Muhammad Arshad3,Asam Riaz4 and Mahmood Ahmad Khan5
...ared to black cumin (Nigella sativa oil; NSO) and insulin. Alloxan induced diabetic rats were post treated with plant extracts (150 mg/kg) and serum samples were collected from overnight fasted diabetic rats on day 15 to observe thepercent changes in blood glucose levels and lipid profile. Garlic extracts have shown the significant (p<0.001) hypoglycemic activity with no considerable effect on mean body weight of rats (ns, p>0.05) as compared to d...

 Mohammed Mahbub Iqbal*, Mehedi Hasan Kanon, Mohammad Amzad Hossain, Abul Hossain, Shamima Nasren, Md. Jahidul Islam, Md. Arifur Rahman

...xecuted to assess the indigenous fish assemblage of Konoskhaihaor for 9 months from May 2014 to January 2015. The Konoskhaihaor area covers 104.50 acres of land containing 6 small to large beels. In the monsoon haor remains under water. A total of 37 fish species belong to 7 orders including prawns were found. Among the 37 species 5 were vulnerable, 7 endangered, 1 critically endangered, 3 exotic, 20 not threatened and 1 not evaluated according to IUCN, Bangla...

Muhammad Jamal Nasir*, Anwar Saeed Khan and Said Alam 

...ccurred. Farmers have indigenous knowledge of the climate of the area to predict and forecast the changing weather and climate. Knowing such traditional knowledge provides an opportunity to understand how the farmers are adapting to new type of weather. The study was carried out in Balambat Tehsil District Dir Lower. They study is primarily based on questionnaire survey and focus group discussion. A total of fifteen villages out of total 173 were selected for ...
Jo Ik-Hwan1, Choi Kwang-Won1 and Muhammad Fiaz2,*
...y matter (DM) and total digestible nutrient (TDN) yield was not different (P>0.05) from control, whereas rye-vetch mixture had increased (P<0.05) crude protein (CP) yield and carrying capacity for Hanwoo heifers than that of rye monoculture. DM and TDN yield under 100 kg manure was higher (P<0.05) than control and 50 kg N/ha levels but not different (P>0.05) with that of 150 kg N/ha. Manure levels did not affect (P>0.05) protein yield. The carry...

Syed Asif Imran Shah1*, Shah Jehan Khan2, Kalim Ullah1 and Obaid Ullah Sayal

...s) were depicted having Eigen value >1 and cumulative variability of 78.66%; PC1 (37.19%), PC2 (12.94%), PC3 (11.62%), PC4 (9.60%) and PC5 (7.32%) of variability. The attributes of significance depicted in PC1 were seed cotton yield, seed index, lint index, sympodia plant-1 and bolls plant-1divulged great contribution towards total variability. Score plot revealed that Baghdadi, CIM 622, SLH 8, BH 184, Sitara 008, CEMB 66, Leader 5, Cyto 178, FH Lalazar, CR...
Sumaira Zareen1, Syeda Sadaf Zahra2, Ayeza Mehmood3, Muhammad Asadullah4* and Aish Muhammad5

 

...ltured on standard Murashige and Skoog (MS) medium supplemented with different concentrations and combinations of benzyl amino purine (BAP) or kinetin for shoot induction and Indole acetic acid (IAA) for primary shoot and Indole Butyric acid (IBA) for root proliferation. Best shoot proliferation (4.48 per explant) was observed in MS medium containing 0.2/0.4 mg/L BAP/IAA combination. For rooting of the micro shoots, MS medium was supplemented with 1.0 mg/l IBA...
Yanjie Guo1, Weini Wu1, Hongmei Wang2, Xiao Guo2 and Yongping Xu2,*
...and eliminate foreign antigens, and the process of local immunity in the female reproductive system is regulated by the coordinated integration of the nervous, immune and endocrine systems. The nerves that branch from the inferior mesenteric ganglion (IMG) are a major source of sympathetic nerves, which dominate the immune response of the female reproductive system. Interleukin-18 (IL-18) is an important Th1 cytokine and plays an important role in pregnancy. T...

Saeeda Raza, Muhammad Naeem Safdar, Amer Mumtaz, Nouman Siddiqui, Khalid Nasim and Muhammad Amjad*

...anced baby foods from indigenous food materials utilizing home cooking methods and evaluate the sensory attributes and shelf life at ambient conditions. Four different recipes of baby foods were developed containing 50% cereals, 30% legumes, 10% vegetables and 10% nuts and seeds. Different processing methods cleaning, washing, germination of pulses, peeling, cutting and blanching of vegetables, drying, roasting, grinding and mixing of ingredients were used. Re...

Raania Ahsan* and Zafar Altaf**

...he technology through indigenous capabilities as well as inviting the multi-national companies (MNCs) to bring in the latest cotton production and protection in the country. Bt cotton can play a significant role to enhance agricultural productivity as the productivity of cotton in Pakistan 0.5 t ha-1, as compared productivity of Bt cotton in China 9 t ha_1, which implies a huge cotton productivity gap. This gap can be narrowed down by the adoption of Bt cotton...

Muhammad Athar Khan1, Muhammad Zahid Latif2*, Syed Amir Gilani3 and Ifrah Bukhari4 

...inst each of the five antigens against the serovars. A total of 250 subjects were included in the study. Out of these, 125 subjects were exposed to the rice paddy water where as 125 were not exposed to rice paddy water. The cumulative seropositivity among the exposed is (83.2%) as compared to (42%) among the non exposed to rice paddy field water. The calculated cumulative odds ratio is 6.7 which represent a strong association of the risk for the development of...
Haixia Liu, Yuwei Ye, Yang Li, Xiaolin Liu, Dongmei Xiong* and Lixin Wang
.../i>(Salmonidae) is an indigenous and endangered cold-water fish in Tsinling mountain, China, and only is distributed as spot in the Weihe River and Hanjiang River among Tsinling mountain, To access relationship of populations from the two rivers and the genetic diversity of wild population, 11 microsatellite loci were firstly used to evaluate the genetic characterization using 120 samples from 2 tributaries (ZZ and LX populations) of the Weihe river and 2 trib...
Asad Sultan1, Sahal Ahmad1, Sarzamin Khan1, Rifat Ullah Khan2,*, Naila Chand1, Muhammad Tahir3 and Shakoor Ahmad2
...parameters and nutrient digestibility at both levels of thermal zones. The results of silymarin on growth, nutrient digestibility and hematological parameters were comparatively lower (P<0.05) in broilers during TN and HS zones. The current results indicate that the supplementation of feed with silymarin and ZnO improved the growth performance, blood parameters and nutrient digestibilit...
Muhammad Rasool1,*, Muhammad Zahid1, Muhammad Ismail Khan1, Qadeem Khan2 and Khalid Khan1
...lly diagnosed Surface Antigen of Hepatitis B Virus (HBsAg). Pre-S2 region nucleotide sequence of the sample MR02 was found completely homologous with the Pre-S2 region of already reported subtype “adr4”. This sample showed 1.2 to 20% variation with the other reported sequences. Nucleotide sequence of the Pre-S2 region of the second sample MR03 does not show 100% homology with any of the reported sequences. It differs 2.4 to `18.8 % from the other r...

 A. N. Naqvi and K. Fatima*

INCIDENCE OF LIVESTOCK DISEASES IN NOMAL AND NALTAR VALLEYS GILGIT, PAKISTAN
...ported in the area were digestive diseases, infections, mastitis, reproductive diseases, endoparasites, ectoparasites, wounds, hematuria, respiratory diseases, emaciation, hemorrhagic septic-emia, tumour, blue tongue, cow pox, enterotoxaemia, tetanus, paralysis and arthritis. In precise, endoparasites were found in 25.3% animals followed by respiratory diseases (24.74%). Most of the cattle (2053) and sheep (926) were found affected with endoparasites, whereas ...

 Habib Iqbal Javed, Ashiq Saleem, Javed Fateh, Mozammil Hussain, Naheed Akhtar and Shamim-ul-Sibtain Shah*

DEVELOPMENT OF RESISTANT MAIZE GERMPLASM
...ic out of 350 and 100 indigenous out of 900 were selected. During autumn 1999, out of 250 accessions (progenies) 35 exotic and 10 indigenous could survive under artificial infestation. Under natural infestation, 100 progenies performed little better. The best plants among these progenies were advanced by self pollination. A total of 400 self pollinated cobs (as progenies) were screened during spring 2000 under natural infest...

 Ambreen Akhtar Saddozai, Saeeda Raza* and Shahzad Arshad Saleem**

MICROBIAL COUNT AND SHELF LIFE OF STRAWBERRY JUICE
...keeping the juice in refrigerator and using preservatives. Thus with good storage conditions, the shelf life of the strawberry juice can be increased satisfactorily.

...
EVALUATION OF LEGUME HERBS NUTRITIVE VALUE AS A RUMINANT FEED AND NITROGEN SUPPLY ON SOIL IN WEST TIMOR, INDONESIA
...ic matter (OM) in vitro digestibility were statistically different among the species of legume (P<0.01). In DL the DM and OM were highest i.e., 74.84 % and 74.37%. Meanwhile, the biomass digestibility of DM and OM was affected by the -2 treatments (P<0.01), being highest in CT (2511.4 g DMm and 2172.1 g -2 -2 -2 OMm ) followed by DL (2351.8 g DMm and 2044.9 g OMm ). Based on this study, CT and DL have potential to deve...

 Nasia Batool*, Imtiaz Ahmad Qamar**, Imdad Hussain Mirza** and Muhammad Fateh Ullah Khan**

MIXING LESS PALATABLE GRASSES WITH UREA, MOLASSES AND EFFECTIVE MICROORGANISMS AND ITS EFFECT ON CHEMICAL COMPOSITION AND DIGESTIBILITY IN GOATS
...hemical composition and digestibility in goats. (HC), (CA), (SH) and (DB) were used and the combinations were grass + 4% molasses, grass + 4% urea, grass + 4% urea + 4% molasses, grass + 4% urea + 1:100 EM, grass + 1:100 EM + 4% molasses, grass +1:100 EM + 4% molasses + 4% urea. Proximate analysis of samples was carried out. Crude protein content of mixtures improved as compared with sole grasses. Digestibility of HC supplem...

Tanzila Kazmi*, Abid Ghafoor Chaudhry*, Aftab Ahmed** and Shaheer Ellahi Khan**

FARMERS BELIEFS ABOUT INDIGENOUS FARMING PRACTICES AND SUSTAINABLE AGRICULTURAL DEVELOPMENT
...beliefs regarding the indigenous farming practices and how it is related with sustainable agricultural development. During the study a mixture of qualitative techniques including interview guide(s), focus group discussions (FGDs) and case study were used. The study revealed that indigenous knowledge is a long term process and demands a complete approach to achieve destined goals. On the other hand it was also found that the ...

 Mustaring,* I. Subagyo,** Soebarinoto** and Marsetyo*

 
GROWTH, YIELD AND NUTRITIVE VALUE OF NEW INTRODUCED BRACHIARIA SPECIES AND LEGUME HERBS AS RUMINANT FEED IN CENTRAL SULAWESI, INDONESIA
...er (DM) yield, in vitro digestibility and nutrient contents. Plant height and tillage number were monitored at week 4, 6 and 8 then harvested at week 8. Results revealed that the Brachiaria mutica (8 weeks old) had highest plant height (207 cm), but lowest tillage number (64), crude protein (CP) content (8.64%), in vitro organic matter (OM) digestibility (47.36%) On the other hand, B. mulato had highest tillage number (117) ...

 Frasat Saeed*, Jehanzeb Farooq*, Abid Mahmood**, Tassawar Hussain***, Muhammad Riaz* and Saghir Ahmad****

GENETIC DIVERSITY IN UPLAND COTTON FOR COTTON LEAF CURL VIRUS DISEASE, EARLINESS AND FIBER QUALITY
...d first five PCs having eigen value >1 explaining 67.8% of the total variation with days to st -1 1 square and flowering along with plant height and sympodia plant which were being the most important characters in PC1. Cluster analysis classified 101 accessions into five divergent groups. The genotypes in st cluster 1 only showed reasonable values for days to 1 square and flower, sympodia per plant, ginning out turn, staple length and fiber fineness and the...

 Farrukh Saleem*, Babar Hilal Ahmad*, Saleem Zahid* and Bilal Kabeer*

COMPARATIVE PRODUCTIVE PERFORMANCE OF INDIGENOUS NAKED NECK AND NAKED NECK CROSSBRED LAYER CHICKENS
...uctive performance of indigenous Naked Neck (NN) and Naked Neck Crossbred (NNC) i.e., Naked Neck x White Leg Horn at Poultry and Wildlife Programme, National Agricultural Research Centre (NARC), Islamabad, Pakistan. A total of 72 birds (16 weeks of age) comprising 30 females and 6 males of each breed were divided into six experimental units / replicates (10 females and 2 male / replicate). Separate breeding pen were allotted to each replicate. The birds were f...

 Maimoona Bashir*, Imtiaz Ahmad Qamar*, Muhammad Fateh Ullah Khan* and Raheel Babar*

EFFECT OF MIXING LOW PALATABLE GRASS OF HETEROPOGON CONTORTUS WITH IPIL IPIL LEAVES ON DIGESTIBILITY IN GOATS
...h sole species on their digestibility in small ruminants. Goats fed II , HC II , HC II , HC II and HC had similar 100% 25% 75% 50% 50% 75% 25% 100% dry matter (DM), crude protein (CP) and crude fibre (CF) consumption among all the treatments. The digestibility percentage of dry matter intake (DMI) varied among the treatments ranging from 68.25% to 41.66%. Mixtures of low palatable grass and Ipil ipil were in general more d

 Muhammad Arshad Ullah*, Nazir Hussain**, Helge Schmeisky*** and Muhammad Rasheed**** 

IMPROVING FODDER QUALITY OF PANICUM GRASS THROUGH INTERCROPPING OF LEGUMES AND THEIR INOCULATION
...so suggested that total digestible nutrients (TDN) were increased by 2-4% as compared to other treatments.

...

 Ambreen Akhtar Saddozai*, Amer Mumtaz*, Saeeda Raza* and Shahzada Arshad Saleem** 

MICROBIAL COUNT AND SHELF LIFE OF PHALSA (GREWIA ASIATICA) JUICE
...lsa juice storage at refrigerated temperature with/without preservatives is recommended. 

...

 Doulat Baig*, Fida Mohammad Abbasi*, Habib Ahmed*, Maqsood Qamar** and Muhammad Ayub Khan**

RESPONSE OF SUNFLOWER HYBRIDS TO DIFFERENT NITROGEN LEVELS FOR PHYSIOLOGICAL AND AGRONOMICAL TRAITS UNDER FIELD CONDITIONS
...s in Pakistan. Mostly indigenous sunflower hybrids are cultivated which give low achene and fodder yields. The issue related with these hybrids ascribed to lack of information about use of inputs and cultural practices. Judicious nitrogen use and suitable high yielding hybrid play key role in increasing sunflower productivity. Protein is the basic requirement of the metabolic processes for the vegetative, reproductive growth and yield of the crop. The protein ...

 Azhar Usman Ali*, Ghulam Sarwar*, Muhammad Aftab* and Sher Muhammad**

EFFECT OF SOIL AND FOLIAR APPLIED COPPER ON GROWTH AND YIELD OF WHEAT (TRITICUM AESTIVUM L.)
...centration by using wet digestion method. Results of the experiment indicated that soil application of copper proved better than foliar application in increasing the yield and yield components of wheat. Similarly, foliar application -1 -1 of copper at 4mg kg (T ; level 2) was better than 2 mg kg (T ; level 1). 

...

  Sohaib Arshad*, Sarwat Naz Mirza*, Imtiaz Ahmad Qamar** and Maqbool Shahbaz**

DETERMINATION OF FORAGE QUALITY OF INDIGENOUS AND EXOTIC RHODES GRASS ACCESSIONS UNDER RAINFED CONDITIONS
... different exotic and indigenous varieties of Rhodes grass were tested for quality and yield parameters. Three varieties were imported from Australia and Zimbabwe namely Sabre, Tolgar (Australian) and Katambora (Zimbabwean). Dry matter yield and crude protein content of these grass varieties were analysed at three growth stages namely vegetative, flow-ering and maturity. These grasses have shown significant differences in fresh and dry matter -1 yield. Fresh m...

 

Shakir Ullah*, Aish Muhammad*, Iqbal Hussian*, Hafeez-Ur-Rahman**, Muhammad Zeeshan Hyder***, Muhammad Din**** and Nizamud Din

MORPHOLOGICAL VARIATIONS IN APRICOT CULTIVARS GROWN IN GILGIT BALTISTAN PAKISTAN
...s
used. The eigenvalue was able to represent 84.8% of total variance. Leaf
size and leaf blade shape were highly correlated (r = 0.383). Positive
correlation found in shape of fruit, weight and percentage of moisture (r =
0.336 and 0.272). Significant correlation was found between fruit weight,
percentage of moisture (r = 0.681) shape of stone (r = 0.067). The result of
this study may be u...

 Abdul Rehman Khan*, Muhammad Mahmood–Ul-Hassan**, Rizwan Ahmad** and Anjum Munir ***

ARSENIC III TOLERANCE POTENTIAL OF FUNGI ISOLATED FROM POLLUTED AND NON-POLLUTED SOILS OF PAKISTAN
...nic (As) tolerance of indigenous filamentous fungi was explored. Fungal strains were isolated from peri-urban soils of Multan and Gujranwala irrigated with untreated industrial or municipal effluent. Some fungal strains were also isolated from non-polluted soils of Islamabad area. Arsenic (III) tolerance potential of 18 fungal isolates was tested by growing them on Potato Dextrose Agar (PDA) medium amended with different concentrations of As (50 to 5600 mg/L)....
Youbao Zhong1, Xianlai Zhang1, Xiaofen Hu1 and Yong Li1, 2,*
...he major site of animal digestion and absorption; however, little is known about the morphological characteristics of the hedgehog’s small intestine. This study was conducted to investigate the microstructure of the hedgehog’s small intestine and mast cell distribution by conventional HE and modified toluidine blue staining, respectively. Despite its similarity to the general mammal small intestine microstructure, the present results indicated that...

Mohammad Nazeer* and Safdar Hussain Shah 

...ogical traits of four indigenous goat breeds of Khyber Pakhtunkhwa (KP) province of Pakistan. The breeds selected for this study were namely Damani, Surguli, Kaghani and Gaddi. Data was collected from two districts to the south (Dera Ismail Khan and Kohat) and two districts to the north (Kaghan and Naran) of KP province. Total number of 120 goat specimen (30 per breed) was selected for this study from random locations. A pretested questionnaire was used to rec...
Xiaopeng Tang1,3, Rong Xiang1, Sijia Chen1,3, Shufen Yang1,3, Hu Liu1,3, Rejun Fang1,3,* and Aike Li2,
...e fractions, and the AA digestibility and metabolic energy of fermented cottonseed meal (FCSM) and enzymatic hydrolyzed cotton seed meal (EHCSM) in white leghorn roosters. Firstly, CSM were fermented with Aspergillus niger, or hydrolyzed with alcalase and flavourzyme. Secondly, a total of 32 white leghorn roosters with similar body weight (1.95± 0.14 kg) were randomly assigned into 1 of 4 treatments, 1) starvat...
Abdul Nabi Jatt1,*, Sarfraz Ali Tunio1, Shaista Bano Memon1, Abdul Sattar Qureshi2 and Muhammad Aqeel Bhutto
...he bacterial strain Shigella dysenteriae IM using API-ZYM test system. Out of nineteen different enzymes, S. dysenteriae IM strain was capable to poroduce nine enzymes, i.e., alkaline-phosphatase, acidic-phosphatase, lipase, valine arylamidase, cystine arylamidase, napthol-AS-BI-phosphohydrolase, β-galactosidase, leucine arylamidase, and trypsin. While, esterase, esterase lipase, α-chymotrypsine, α-galactosidase, β-gl...
Basit Zeshan1,2,*, Mushtaq A. Saleem2,Javed Iqbal Wattoo2, Mohd Mokhtar Arshad1 and Maizan Mohamed1
...sub> (containing five antigenic sites located at 24–61, 291–398 and 497–543 amino acid residues of S1 glycoprotein) were amplified by overlap PCR, cloned into prokaryotic expression vector resulting pET-Sf200 and confirmed the construct by sequencing. The recombinant plasmid was identified by restriction enzyme and sequencing analysis. The in vitro expression of the truncated protein was analyzed in E. coli with a mol...
Saba Parveen Samo1, Moolchand Malhi1,*, Javed Gadahi2, Yan Lei3, Allah Bux Kaciwal1 and Saeed Ahmed Soomro1
...t; 0.05) compared to A. Digestibility trails revealed that utilization of dry matter (DM), crude protein (CP) and crude fiber (CF) increased (P < 0.05) by 13.71, 12.02 and 4.78% at week 3 and by 11.11, 11.8 and 3.46 % at week 6 in B compared to A. Among carcass characteristics the carcass dressing % (52.99 ± 0.77 vs 49.41 ± 0.6 ) and leg weight (kg) (1.28 ± 0.06 vs 1.05 ± 0.04) increased (P < 0.05) in B compared to A. The phys...
Grace C. Onyishi, Ifeanyi Oscar N. Aguzie, Christopher D. Nwani*, R.N.N. Obiezue and I.C. Okoye
... temperature in Nsukka, Nigeria. Hospital records from three hospitals in Nsukka town: Bishop Shanahan Hospital, University of Nigeria Medical Centre and Sadiq Hospital were examined for malaria prevalence and intensity from January to July 2015. Indoor biting Anopheles mosquitoes were collected by standard procedures within the study duration. Meteorological data on relative humidity and temperature was obtained from...
Grace Chinenye Onyishi, Ifeanyi Oscar Aguzie, Joseph O. Okoro, Christopher Didigwu Nwani*, Ngozi Ezenwaji, Ndubisi Stanley Oluah and Fabian C. Okafor
...y of snails consumed in Nigeria is affected by parasites they harbour. Some of these parasites endanger humans as well. This study was conducted to determine the terrestrial snail species and associated helminth parasites in Ugwueme agricultural zone of Enugu State, Nigeria. The snails were collected from two communities. Light and teasing methods were used for recovery of parasites from the snails. A total of 618 snails bel...
María Magdalena Crosby-Galván1, Bernardino Espinoza-Velasco1 and Mónica Ramirez-Mella2,*
.../i>) residue in ruminal digestibility and gas production in an in vitro assay. Four treatments were evaluated: a control using a standard diet for ruminants elaborated with corn stover, corn grain and soybean meal, and three experimental diets with 10, 20 and 30% dry residue of chihua pumpkin instead of corn stover. Dry matter digestibility and gas production was determined at 24 h of incubation in ruminal fluid obtai...
Shahzad Mahmood1, Memuna Ghafoor Shahid1, Muhammad Irfan2, Muhammad Nadeem3*, Quratulain Syed3
... done by Aspergillus niger for the production of α–amylase. The crude enzyme produced was further characterized. The results showed that enzyme had an optimum pH of 6.0 and stable in range of 4.0-6.0. Optimum temperature of enzyme was 40oC and attained 96% activity at 40oC for 70min. Metal profile showed that α-amylase activity was enhanced in the presence of Ca2+, Cs1+, Mn2+ ...
Olaniyi Alaba Olopade1,*, Henry Eyina Dienye1, Bosede Jimba1, Nathanael Akinsafe Bamidele2, Iyabo Olusola Taiwo2
...River and Buguma Creek, Nigeria. Assessments of the length and weight parameters indicated that the total length ranged from 12.4 cm to 29.8 cm with a mean value of 17.84±0.40 and the weight varied between 41g to 640g in subject collected from New Calabar River population. In Buguma Creek, the total length ranged from 10.9 cm to 23.5 cm with a mean value of 14.99 ± 0.26 and the weight was from 21g to 520g. The b value varied between 2.96, wh...
Riyadh S. Aljumaah*, Mohammed A. Alshaikh, Abdulrahman Jarelnabi, Mutassim M. Abdelrahman and Mansour F. Hussein
Mei Li1, Di Zhang2 and Wan-Long Zhu1,*
...energy intake (GEI) and digestible energy intake (DEI). On day 28, E. miletus fed diets containing tannic acid showed no significant changes in RMR, GEI and DEI. The apparent digestibility and NST were kept stabled during the experiment. These results indicated that tannic acid can increase RMR and reduce energy intake within a short period and the changed physiological function returned to baseline for a long period....
Sherin Reda Rouby
...he LSDV is similar in antigenicity and in cultural characteristics to SPPV. The current study was delineated to identify capripoxviruses from different clinical samples and differentiate capripoxviruses without the need of gene sequencing. A total of 16 lumpy skin disease clinical samples (skin nodules, scabs, buffy coat, lymph aspirate and engorged ticks) and three sheep pox biopsy skin samples were subjected to DNA extraction followed by 30 kDa RNA poly...

Hamza Khan1, Safdar Ali1, Ijaz Ahmad*2, Ihsanullah Khan3, Shujaat Hussain1, Bashir Ahmad Khan4 and Muhammad Suhaib5  

...res for enhancing the indigenous oilseed production through different possible approaches. Development and promotion of local sunflower hybrids can be one of the options. Local sunflower hybrids with higher yield and wider adoptability can make Pakistan self sufficient in edible oil. Therefore a study was carried out at National Agricultural Research Centre Islamabad, to evaluate the performance of 12 locally developed sunflower Hybrids. Twelve sunflower hybri...

Osama Elshazly1, AbdelSatar Arafa1, Mohammed A. Rohaim2, Ismaeil M. Reda2 and Hussein A. Hussein2*

...isolation, as well as antigenic and molecular detection methods. Full-length hemagglutinin (HA) sequences of six representative H5N1 isolates were analysed to study their genetic evolution followed by estimation of their evolutionary rates among different virus clusters. This analysis revealed a high evolution rate for clade 2.2.1.2. Additionally, analysis of selection pressures in the HA gene revealed a positive selection pressure. Deduced amino acid analysis...
Amna Kausar1,2, Sana Anwar1, Naila Siddique2, Safia Ahmed1 and Javid Iqbal Dasti1,*
... desi birds (n=12; 5%), pigeons (n=9; 3.6%), desi chicken (n=9; 3.6%), broiler breeder (n=3; 1%) and layer (n=2; 0.8%). Overall sero-prevalence was 53% and average range of the hemagglutination inhibition (HI) antibody titer (MT log2) against AIV H9 remained 7±3. Real-time PCR results showed 8.3% (42/507) of the samples were positive for viral matrix gene. Sub-typing was performed by specific anti-sera. PCR amplification was done by using N2 ...

Baharullah Khattak1*, Saifullah2, Shaukat Hussain2, Musharraf Ahmad2, Asad Ali2, Mohammad Junaid2, Ijaz Ahmad Khan3, Taj Ali Khan1 and Mubbashir Hussain1 

...Averages, divided the indigenous isolates of Trichoderma, into four well-defined clusters. These results clearly revealed that there were considerable genetic differences among the Trichoderma isolates. While, there was no correlation, found between RAPD clusters and aggressive groups (AGs). Isolates belonging to different AGs were found scattered among the four RAPD clusters. It was concluded that the indigenous isolates of...
Asad Sultan1,*, Shahroom Khan1, Sarzamin Khan1, Naila Chand1, Muhammad Shuaib Khan2 and Hamza Maris3
...ed efficiency, nutrient digestibility, and tibial mineralization of Ca and P in broiler.
...
Nadia Saeed1,Mian Sayed Khan2, Habib Ahmad3, Muhammad Abdullah4 and Muzafar Shah5,*
...rent organic amendments pigeon manure, horse dung, cow dung, duck manure and castor oil cakes at the rate of 8 Kg/tree and saw dust at the rate of 10kg/tree, respectively. Untreated trees were kept for the comparison. Soil samples and sub samples of both treated and untreated trees after 3, 6 and 12 month from Abbottabad and Mansehra by using Bearmann funnel technique. After Quantitative analysis of both treated and untreated trees, it was observed that treate...
Tariq Mukhtar
...t evaluation that the indigenous isolates of T. harzianum and T. viride have the potential to control M. incognita.
...
Jun Chen1, Shuai Shang2, Xiaoyang Wu1, Jiakuo Yan1, Huaming Zhong1, Huanxin Zhang2, Weilai Sha1, Wanchao Zhu1 and Honghai Zhang1,*
...m southern Africa has a digestive system that allows it to absorb and digest large amounts of plant material through microbial fermentation in the hindgut. So far, there has been no study of the gut microbiota of the cape oryx. Here, we provided the first description of the fecal bacterial populations of the cape oryx by using high-throughput Illumina sequencing technology. We analyzed 100,180 high-quality sequences of the 1...
Danyu Zhang1, Ying Liu1, Qinxin Shi1, Zhiwei Peng1, Yan Hua1 and Zhijun Hou1,2,*
...diversity than Siberian tiger, panda, horse, and human, and it may originate from the more divertible diet of the sable.
...
Souheila Slimani1,*, Sonia Hamouda1, Chahrazed Souadi1, Sara Silini2, Cherif Abdennour2 and Leila Delimi3
...uction of male domestic pigeons Columba livia domestica, subjected to a long photoperiod (19L: 05D). The fungicide was orally administered at 5 and 10 mg/Kg body weight/day for 10 consecutive weeks.Testicular volume and weights were measured weekly, whereas semen quality, and histopathological profile were investigated at week 10.The obtained results reveal that under a long photoperiodthe sexual activity of the control lasted only 04 weeks, characteriz...
Zihong Chen1, Ling Xu2, Xiaona Yang2, Yaguan Zhang3* and Yuming Yang4
...ters combined with a multigene phylogenetics analysis involving 5.8S–ITS, nrSSU, nrLSU, EF–1α, RPB1 and RPB2. In multilocus phylogeny, M. baoshanense was grouped as a sister clade to Metarhizium majus, Metarhizium guizhouense, Metarhizium lepidiotae and Metarhizium indigoticum, but conclusively separated from these allied species with high support value. Morphological character differentiated M. baoshanen...
Changqing Zhao*, Zhuochi Chen and Yubin Li
...aste were improved, and digestion and absorption were enhanced.
...
Syed Makhdoom Hussain1,*, Muhammad Zubair ul Hassan Arsalan1Arshad Javid2, Abdullah Ijaz Hussain3, Nosheen Aslam4, Qasim Ali5, Majid Hussain6Muhammad Masoom ul Hassan Rehan7, Muhammad Mudassar Shahzad1,8Anam Khalid1 and Danish Riaz1
...erformance and nutrient digestibility in L. rohita fingerlings. MOLM was added in the diet by replacing fish meal at the levels of 0%, 10%, 20%, 30% and 40%. Triplicate tanks were used for each treatment having 15 fingerlings. These fingerlings fed at the rate of 5% of live wet weight. Chromic oxide as an inert marker was also added in the diets. Feces from each tank were collected twice a day to estimate the nutrient dige
Syed Makhdoom Hussain1,*, Nosheen Aslam2, Arshad Javid3, Shehzadi Liaquat1,Muhammad Mudassar Shahzad1, 4, Muhammad Zubair-ul-Hassan Arsalan1 and Muhammad Adnan Khalid1
...of fish. Higher mineral digestibility (Ca 76%, Fe 62%, Zn 57%, P 76%) and carcass composition (crude protein 17% and crude fat 10%) was found in fish fed on 3 gKg-1 level of probiotics supplemented canola meal based diet. Whereas higher ADC% of Na (75%), K (67%) and Mg (62%) were recorded by fish fed on 4 gKg-1 level of probiotics supplemented canola meal based diet. Similarly maximum improvements in RBCs, WBCs and Hb as well as higher mi...

Ghulam Abbas1, Muhammad Jawad Asghar1, Muhammad Rizwan2*, Muhammad Akram1, Jaffar Hussain1 and Fiaz Ahmad1 

...ifty eight exotic and indigenous diverse mungbean genotypes were evaluated for seed yield and other related traits. The genetic analysis of mungbean germplasm revealed high genotypic (GCV) and phenotypic (PCV) coefficients of variability for biological yield (GCV% = 31.70, PCV% = 33.58), harvest index (GCV% = 27.80, PCV% = 30.16) and seed yield (GCV% = 25.28, PCV% = 27.54). While heritability estimates were high for all the traits except days to maturity and c...
Muhammad Mudassar Shahzad1,2, Syed Makhdoom Hussain2,*, Farhat Jabeen2, Abdullah Ijaz Hussain3, Arshad Javid4, Muhammad Asrar2 and Muhammad Zubair-ul-Hassan Arsalan2
...plementation on mineral digestibility and carcass composition of Catla catla fingerlings fed MOSM based diets. Phytate in plant by-products decreases the bioavailability of minerals and deposition of nutrients in fish body, resulting in poor fish growth. Results demonstrated that phytase supplementation showed significant (p<0.05) improvement in ADC% of minerals and carcass composition of fish. Maximum diges...
Irshad Ali1, Mohammad Masood Tariq2,*, Abdul Waheed2, Ferhet Abbas Yousafzai1, Farhat Abbas Bokhari1, Majed Rafeeq1, Muhammad Ali1, Muhammad Adnan Attique1, Shahid Amin3 and Tahir Hameed1
Mudassar Javed1,Muhammad Zeeshan Majeed1,*, Muhammad Sufyan1, Sajjad Ali2 and Muhammad Afzal1
...borer (Helicoverpa armigera) cause substantial quantitative and qualitative losses to okra crop. In this study, 18 pesticides including conventional and new-chemistry synthetic insecticides and different botanical extracts were screened against these pests on okra variety ‘Pusa Sawani’ under field conditions during 2015 and 2016 in a randomized complete block design. The average number of lepidopterous larvae in plots treated with the...
Kamran Khan1, Sarzamin Khan2, Nazir Ahmad Khan2,* and Naseer Ahmad2
...ity of rabbits. Sixty indigenous rabbits were divided randomly into five treatment groups. The control group was fed on standard concentrate basal diet, whereas in the treated groups, the basal diet was supplemented with low (3.5%; LCF) or high (7%; HCF) level of crushed flaxseed and low (1.5%; LFO) or high (3%; HFO) level of flaxseed oil until slaughtering (day 90). Carcass fat deposition was greater (P < 0.05) in the supplemented groups as compared to the...
Rana Zeeshan Habib1, Muhammad Afzal1, Syed Zakir Hussain Shah2,*, Mahroze Fatima3, Muhammad Bilal4 and Syed Makhdoom Hussain5
...lementation on minerals digestibility of Labeo rohita fingerlings fed on canola meal based diet. Nine experimental diets were prepared by supplementing CA (%) and PHY (FTU/kg) at the level of 0, 0 (control); 0, 1000; 0, 2000; 1.5, 0; 1.5, 1000; 1.5, 2000; 3, 0; 3, 1000; 3, 2000 respectively. Chromic oxide was used as inert marker in diets to estimate mineral digestibility. Fish fed on PHY supplemented diet showed high...
Wang-Dui Basang1, Tian-Wu An2, Luo-Bu Danjiu3, Yan-Bin Zhu1, Shi-Cheng He3, Xiao-Lin Luo2, Wei-Wei Ni4, Xiao Wang4, Shu-Zhu Cheng4, Jian Wang4 and Guang-Xin E4,*
... grunniens) is an indigenous domestic animal living at high altitudes in the Tibetan plateau that is economically important for the Tibetan people. In this study, we investigated the diversity and phylogeography of four geographic ecotype populations of the Naqu yak (133 individuals) using an 811-bp mitochondrial DNA D-loop region sequence. In total, 57 polymorphic sites, including 54 single-nucleotide polymorphisms and 3 single-nucleotide copy number vari...

Raphael Olanrewaju Babatunde, Adeyemi Esther Omoniwa and Miriam Ukemenam 

...ments programmes by the Nigerian Government and development institutions over the years to bridge the gender gap in schooling among children and increase enrolment in schools, most children are still found outside the walls of the classroom especially in rural areas. This research assessed how inequality in gender as it regards schooling among children affects the income as an indicator of livelihood of farming households in Kwara state, N

Irfan Ali Sabir1, Saeed Ahmad1*, Muhammad Nafees2, Ahmad Sattar Khan1, Maryam3 and Ishtiaq Ahmad

...ial and noncommercial indigenous and exotic mango varieties were selected to collect and analyze fruit morphological data under randomized complete block design (RCBD). There was significantly high fruit weight and pulp contents in ‘Kensington Pride’. Total soluble solids (TSS) were higher (21.9 B°) in ‘Chaunsa’, while, titratable acidity (TA) was lowest in ‘Lambay Alfansu’ and ‘Momi-K’. However, reducing sug...
Muhammad Mobashar1, Muhammad Tahir1, Shahbaz Javaid2,*, Muhammad I. Anjum2, Insha Gul3, Nazir Ahmad1 andAbdul Sami1
.../sup>In vitro DM digestibility (%) significantly increased (P<0.05) in ration II compared to ration I and III. Intake of minerals (g/day) of Calcium and Magnesium did not vary significantly, however intake of Manganese, Copper, Zinc and Ferrous decreased with advancement in oat growth. Milk yield (Kg/day) from rations II was significantly higher (P<0.05) than rations I and III, while milk composition was not significantly affected. The DM yield of...
Kiran Aqil1, Muti-ur-Rehman Khan2, Asim Aslam2, Aqeel Javeed5, Rizwan Qayyum1, Farooq Yousaf,1 Farkhanda Yasmeen1, Muhammad Luqman Sohail3 and Sajid Umar4,*
... of six dilutions of Nigella sativa (N. sativa)alcoholic extracts against the PPRV was investigated in vitro. Vero cell lines infected with PPRV were treated with six dilutions alcoholic extracts of N. sativa (200, 100, 50, 25, 12.5 and 6.25 µg/mL). The antiviral effects were judged by 3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-di-phenytetrazoliumromide colorimetric (MTT) assay. Plaque reduction assay was used to test the mode of action. ...
Binbin Shan, Yan Liu, Changping Yang, Shengnan Liu and Dianrong Sun*
...ical alignment, 13,083 unigenes had significant hits in Nr database. And then, 10,467 unigenes were annotated into three ontologies: biological processes, cellular components, and molecular functions. Moreover, 18,621 unigenes were mapped 25 different clusters of eukaryotic proteins. In addition, a total of 17,671 unigenes were classified into 311 KEGG p...
Majid Hussain1, Syed Makhdoom Hussain1,*, Razia Iqbal2, Arshad Javid3, Muhammad Mudassar Shahzad1,4 and Muhammad Zubair-ul-Hassan Arsalan1
...gical indices, nutrient digestibility and growth performance of Catla catla fingerlings. MOLM based diet was sub-divided into one control diet (0% CA) and five test diets, each supplemented with 1%, 2%, 3%, 4% and 5% CA, respectively. Chromic oxide as an inert marker was added in the diets to determine nutrient digestibility. The fingerlings were fed twice daily at the rate of 5 % of live wet body weight on their resp...
Iqbal Ahmad Alvi, Muhammad Asif, Rabia Tabassum, Zaigham Abbas and Shafiq ur Rehman*
...ted, that a standard refrigeration is suitable and economical means of long term storage with no fall of phage activity.
...

Zulqarnain and Zafar Iqbal* 

...illium sp., Aspergillus niger, Aspergillus flavus, Trichoderma harzianum, Alternaria solani and Pythium aphanidermatum showed growth inhibition (%) against the pathogen. Penicillium sp. showed maximum growth inhibition (63.85 ± 3.26 %) and its extract showed 9.20 ± 0.14 mm zone of inhibition at 1000 mg·L-1 against the Fusarium wilt. Based on its higher in vitro antifungal activity, in vivo screen house experiments were conducted. Plants gi...

Bushra Qadir1, Muhammad Asim2 and Mohammad Akmal1* 

...y matter (DM) and total digestible nutrient (TDN). Green forage availability fluctuates in months of the year due to prevailing climate. Alfalfa, with higher potential, can be adjusted in the cropping system for supplying green forage around the year. Nonetheless, its initial growth for stand establishment is a major issue. Treatments of the experiments were four alfalfa varieties (i.e. UCD, Dura, Vernal and Cuf 101) whose seed were treated with overnight in b...

Bata Shalangwa Ishaku1*, Maimadu Abdullahi1, Dakwang Nalong1, Rengkat Jonah1 and Olabode Mayowa

...r in Jos, Plateau State Nigeria. Five hundred (500) serum samples comprising of 300 pigs, 100 each of sheep and goats were collected and analyzed for Toxoplasma gondii antibodies (IgG) using latex agglutination test (LAT). Serum samples with LAT titer >10 IU/ml were considered positive. The study showed that 176 of the 500 samples analyzed were positive for Toxoplasma gondii antibodies (IgG) giving an overall prevalence of 35.2%. There was statistically sig...
Moorthy S. Muthuswamy
...enon: backed by the prestige and resources of Saudi Arabia, religious leaders have popularized the appealing theme of sharia as all-encompassing “divine law” to advance radical Islamist agendas. By identifying sharia as the theme and religious leaders as its enablers, such an explanation facilitates our understanding of the causes and backers of this religious radicalism.
...
Song Jiang1,2, Fa-lin Zhou1, Qi-bin Yang1, Jian-hua Huang1, Li-shi Yang1 and Shi-gui Jiang1*
...topancreas of the black tiger shrimp (Penaeus monodon) were investigated via the effects of thermal stress on superoxide anion (O2-) production, superoxide dismutase (SOD) activity, glutathione (GSH) and adenosine triphosphate (ATP) concentration, as well as nitric oxide synthase (NOS) activity and nitric oxide (NO) concentration catalyzed by NOS. Based on biochemical analysis of the above, results showed that O2-

Ocheme Julius Okojokwu1*, Maryam Bisola Adebayo2, Bashiru Shafa Abubakar3, Ibrahim Abubakar Yusuf2 and Joseph Aje Anejo-Okopi1  

...enatal clinic in Abuja, Nigeria. Structured questionnaire was used to obtain data on socio-demography and risk factors. The sera were obtained from samples were analyzed for IgG and IgM antibodies of human parvovirus B19 using SERION ELISA Classic Parvovirus B19 IgG/IgM kits. A total of 171 (52.5%) of women had IgG antibody to Parvovirus B19 while 31 (9.5%) were positive for IgM antibody. Demographics including age group, educational status, occupation, type o...

Waqas Ali* and Mudasser Habib 

...ted in known critical antigenic region but as a whole protein was under negative selection pressure for serotype O, A and Asia 1. Therefore, under mass vaccination campaigns and drastic environmental conditions strains under negative selection has the ability to make escape mutants. 

...
Muhammad Zahid1, Aneela ZameerDurrani1, Muhammad Ijaz1, KhushiMuhammad1,Muhammad Usman1,*, Muhammad Husnain1 and Nadeem Kamal2

 

...emeral fever (BEF) in indigenous breeds of bovine was 63.33%, lower, than exotic breeds 64.66% which were found to be more prone to disease. In the case of buffalo breeds, prevalence was more in Nili Ravi buffalo, i.e. 36.6% than Kundi breed, i.e. 33.3%. Prevalence of disease was found maximum in humid and plain area of Lahore, i.e. 56%. In dry, dusty and hot weather of Multan overall the prevalence of disease was 55.5%, while in Rajanpur ...
Tahira Batool1*, Nabila Roohi1, Athar Mahmud
...eshawari varieties of indigenous Aseel chicken. A total of 240 day-old chicks, 60 from each of the variety were taken randomly and sub-divided equally into three groups (A, B and C). They were offered three lysine regimens i.e., L1 constituting 1.3% lysine from 0-6th week (1-phased) to group A and L2 containing 1.4-1.2 % lysine where 1.4% lysine was offered from 0-3rd week and 1.2% lysine from 4-6th week (2-phased) to group B, ...
Adem Aksoy1, Yakup Erdal Ertürk2, Ecevit Eyduran3 and Mohammad Masood Tariq4,*
...tudy was conducted on indigenous Morkaraman breed and Romanov × Morkaraman F1 Crossbreed sheep reared under semi-intensive conditions in order to determine the influence of dam age, genotype, birth weight, year, sex and birth type at lambing, weaning weight, weaning age, grazing period and weight at the end of the grazing period on pasture revenue, which is defined as the obtained revenue per lamb from weaning period to the end of the grazing period. Mul...
Ejaz Mahmood Ahmad Qureshi1,*, Amtul Bari Tabinda2, Seemal Vehra3 and Atif Yaqub4
... and subjected to NS1 Antigen test (ELISA). To determine infectivity in a mosquito population, detection of the viruses in adult mosquitoes was used to calculate Minimum Infection Rate (MIR). Average MIR was highest being 3.52 and 2.94 in late rainy season in 2011 and 2012, respectively, while it was highest (3.06) in early post rainy season in 2013. Results also revealed significant correlation coefficient ‘r’ and coefficient ofdetermination r
Zafar Iqbal*, Farah Ansar, Zil-e-Huma
...oecilia reticulata; tiger oscar Astronotus ocellatus; koi carp Cyprinus carpio; giant gourami Osphronemus goramy; blue line shark Pangasianodon hypophthalmus and silver shark Balanitiocheilos melanopterus. The health status of these fishes ranged from being healthy, moribund and infected. The parasites observed from these fish were: seven protozoans species (Chilodonella sp, Trichodina sp; Ichthyobodo

 *Irum Javed1, Muhammad Asgher2, Sadia Noreen1, Humara Naz Majeed1 and Tanzila Sahar1

SOLID STATE FERMENTATION OF ASPERGILLUS NIGER FOR CITRIC ACID PRODUCTION USING AGRICULTURE RESIDUE AS SUBSTRATE
...ric acid by Aspergillus niger using agro-industrial wastes. In the first step of study the liquid state fermentation (LSF) and solid state fermentation (SSF) by Aspergillus niger was compared using molasses based medium and the different carrier substrate. In a second set of study wheat bran was utilized for citric acid production via fermentation parameters was optimized. The results showed significant (P≤ 0.05) differen...

 Sumera Siddique1,†, Hafiz Abdullah Shakir1, Javed Iqbal Qazi1*, Amtul Bari Tabinda2, Muhammad Irfan1

Screening of some agri-wastes for economical cultivation of Candida tropicalis SS1
...sured by micro Kjeldahl digestion system) followed by bagasse, mango, and apple peels with respective values of as 13.38%, 3.20%, 2.69% and 2.58%. Best growth of C. tropicalis SS1 was obtained in aqueous extracts of 2% WM peels and the yeast cells attained up to 33% protein content on dry weight basis. In 2% aqueous extract of WM, the yeast strain yielded maximum biomass up to 6g/L with corresponding absorbance(600 nm) of 3.49±0.05 after 48 h of incubat...

 Mirfa Murtaza1, Sajid Abdullah1, Wardah Hassan1*, Khalid Abbas1, Huma Naz1, Muhammad Anjum Zia2

Studies on amylase and lipase activity in fishes fed with diet containing different feed ingredients
..."> The activity of digestive enzymes was determined in Hypophthalmichthys molitrix, Catla catla and Cyprinus carpio fed
with different feed ingredients. There were two glass aquaria (3×2×2 ft) for each species and ten fish in each
aquarium. Fish in one aquarium were fed on rice polish and fish in second aquarium were fed on corn gluten @ 3 %
of their wet biomass for 70 days. Results showed that fish wet weight varied signi...

 Muhammad Moosa Abro1, Ali Murtaza Dharejo2, Muhammad Munif Khan2, Nadir Ali Birmani2, Saima Naz2*

Description of first record of Petasiger exaeretus Dietz, 1909 (Trematoda: Echinostomatidae) in avian host from Pakistan.
...rst record of genus Petasiger which was collected during systematic study of
helminths of Little Cormorant, Phalacrocorax niger of District Sanghar, Sindh, Pakistan. Petasiger exaeretus (Dietz,
1909) were identified from the intestine of Little Cormorant, Phalacrocorax niger on the basis of morphological
features including pear shape...

 Saima Naz*, Adil Ali Rajpar, Abdul Hameed Chandio

New Records of some Phthiraptera (chewing lice) of birds from urban areas of Hyderabad, Sindh, Pakistan
...Piaget, 1880)
on pigeons and doves; Brueelia subtilis (Nitzsch, 1874), Menacanthus eurysternus (Burmeister, 1838) and
Sturnidocus refractariolus (Zlotorzycka, 1964) on house sparrow; Goniodes dissimilis (Denny, 1842), Lipeurus
tropicalis (Peters, 1931), Menacanthus pallidulus (Neumann, 1912), M. stramineus (Nitzsch, 1818) and Menopon
gallinae (Linnaeus, 1758) on domestic fowls; Brueelia saliemi (Ansari, 1957), Colpocephalum fregili ...

 Jhan Zeb, Muhammad Javed

Forecasting percentage contribution of plankton biomass towards increase in fish yield under composite culture conditions
... 24, 26, 28, 30 and 32% digestible protein (DP), respectively @
2% of their wet body weight daily. However, control fish (T7) although had an access to plankton; but were devoid of
the availability of any supplementary diet. Data on dry weights of plankton biomass and increase in fish yield were
collected on monthly basis and subjected to regression analysis in order to estimate the percentage contribution of
plankton biomass toward...

 Zafar Iqbal*, Hafiza Madhia Imtiaz

Parasites of double tail goldfish, Carassius auratus L. imported to Pakistan
...an un-identified
digenean were also found encysted on gill filaments (infection 32%, mean intensity 3.87). Ichthyophthirius
multifiliis infection on fins was found 20% and mean intensity was found 2.4. Trichodina sp. infection on
fins was 12% (with mean intensity 13). Low infection by Piscinoodinium pillulare on fins (8%) and of
Tetrahymena sp. on fins and gills (4%) was also observed. This study has shown that imported double tail<...

Muhammad Asghar Hassan1, Imran Bodlah1* , Ayesha Aihetasham2 

First record of the oriental species, Saltella setigera Brunetti, 1909 (Diptera: Sepsidae) from Pakistan
...1830, Saltella
setigera Brunetti, 1909 is recorded for the first time from Pakistan. The additional
morphological and genital characters of S. setigera along with its photographs,
distributional notes and remarks are provided.

...

 Khurram Shahzad1*, Muhammad Naeem Khan1, Farhat Jabeen2, Nasreen Kosour3, Muhammad Sohail1, Muhammad Khalil Ahmad Khan1, Munir Ahmad1

Bioaccumulation of manufactured titanium dioxide
...sue samples
were digested with the help of nitric acid (HNO3) and perchloric acid (HClO4). Acid
digested samples were analyzed by Inductively Coupled Plasma Mass
Spectrophotometry (ICP-MS) foraccumulation of Ti, Cu and Zn nanoparticles in
various tissues (gills, liver and muscles). Ti and Cu accumulation in gills was
recorded to be 5.7033 and 3.7133 μg/Kg respectively,whereas, maximum Zn...

Saman Fatima* and Shazia Iram 

...lus flavus, Aspergillus niger, Alternaria sp., Lasiodiplodia theobromae, Fusarium sp. After sequencing of DNA the pathogen species identified includes fungal Colletotrichum sp. These pathogens are involved in deterioration the skin of Citrus reticulate. They can either attack the healthy fruit leading to various infections or they can enter through various lesions caused by physical stress and worsen the condition. 

...

Farkhanda Asad1*, Samina Qamer1, Tayyaba Ali1, Ammara Behzad1 and Tahira Yasmin2 

...on on apparent nutrient digestibility coefficient (%) of gelatinized and non-gelatinized corn in Cirrhinusmrigala fingerlings for 90 days. Using various levels of chromium chloride hexahydrate six test dietsdesignated as; T1(G/0.0, CrCl3.6H2O mg Kg-1), T2(NG/0.0, CrCl3.6H2O mg Kg-1), T3(G/0.2, CrCl3.6H2O mg Kg-1), T4(NG/0.2, CrCl3.6H2O mgKg-1), T5 (G/0.4, CrCl3.6H2O mg Kg-1) and T6(NG/0.4, CrCl3.6H2O mg Kg-1) were prepared. Results showed highest apparent d
Daniel Zaborski,1,* Muhammad Ali,2 Ecevit Eyduran,3 Wilhelm Grzesiak,1 Mohammad Masood Tariq,2 Ferhat Abbas,2 Abdul Waheed4 and Cem Tirink5

Nighat Seema1*, Muhammad Hamayun1, Husan Ara1 and Raham Sheer Khan

... soil except that of A. niger. Similarly, inoculation of endophytic fungi did also increased the value of soil nitrate ions in the range of 26.86±1.34~39.39±1.97 mg kg-1 with or without drought stress. Inoculation of endophytic fungi increased the amount of extractable P and K in growth media compared to soil samples before experiment and after experiment in control samples. In case of micronutrients, only copper was decreased in endophytic treat...

Sahar Shibli1,2*, Farzana Siddique1, Saeeda Raza2, Zaheer Ahsan3 and Irum Raza4 

...lign: justify;">Three indigenous peanut cultivars from Pakistan specifically Local-334, Bard-92 and Bard-479 were investigated in the study for compositional quality and peanut butter development. Chemical composition of peanut cultivars indicated 5.53±0.20 to 5.93±0.02 % moisture, 2.00±0.11 to 2.17±0.05% ash, 49.80±3.54 to 50.90±0.93% fats, 23.83±1.71 to 26.43±1.15 % proteins, 13.23±2.20 to 19.42&...

Mudassar Javed, Muhammad Zeeshan Majeed*, Muhammad Luqman and Muhammad Afzal 

...tella and Helicoverpa armigera (Noctuidae: Lepidoptera), has been one of the contemporary issues of the farmers in Afro-Asian regions. This two-year field study was carried out to evaluate an IPM (bio-intensive) module developed by selecting through in-situ evaluation and incorporating the most effective pest control options along with the biological (parasitoid Trichogramma chilonis egg cards) and cultural techniques against okra lepidopterous borers. Accordi...
Mujahid Niaz Akhtar* and Amjad Farooq
... preys Helicoverpa armigera, Pectinophora gossypiella, Earias vitella populations found on cultivated cotton crops (government approved varieties) in Southern Punjab, Pakistan. Data was collected after ten days of interval during crop season from 2014 to 2016 randomly. Significant Correlations coefficients were observed which can be used for effective pest control program to predict predator prey ratio.
...
Dur-E-Shahwar1,2, Riaz Ahmad Sheikh1, Nazia Jamil2*
...f biosurfactants from indigenous bacteria, isolated from produced water and produced water contaminated soil samples collected from three selected sites of Eastern Potwar, Punjab, Pakistan. Forty seven bacteria were isolated out of which five (F1, F3, F20, F23 and C16) were selected on the basis of high optical densities of 0.7, 70% oil and grease reduction potential and maximum CFU/ml of ≥3 ×106. The weights in g/l of biosurfactants produc...

Ahmed Samy1*, Hala Mohamed Nabil Tolba2, Gamelat Kotb Farag3 and Ahmed Abd El Halim1 

... (NDV), that designated pigeon paramyxovirus 1 (PPMV1) commonly isolated from pigeons (family Columbidae). PPMV1 not only represent a potential threat to pigeon but also it can infect chickens and its virulence enhanced upon passaging in chickens causing clinical signs and deaths. In the present study brain samples were collected from twelve pigeon flock...
Ajmal Khan Kassi1,*, Humayun Javed1 and Tariq Mukhtar2
...ity to Helicoverpa armigera is lacking, therefore, in the present study relationship between physico-morphic characteristics and resistance or susceptibility of okra cultivars to H. armigera was evaluated. The heights and stem girths of comparatively resistant varieties were statistically greater as compared to intermediate resistant and comparatively susceptible varieties. Contrarily, hair densities on leaf mi...

Sabi-Ur-Rehman1, Anwar Khalid2, Qazi Najam Us Saqib3, Farooq Ahmad1, Shaheed-Ur-Rehman4, Neelam Zaman3, Ayeza Mehmood5 and Abdul Samad6*  

...rains were Aspergillus. niger, A. flavus, and D. turcica. The maximum zones of inhibition, 23.00±0.56 mm and 25.00±0.50 mm was given by ethyl acetate fraction of leaves against P. fluorescens and A. niger respectively. The crude saponins isolated from leaves did not give significant results against the tested bacterial strains where as it was significantly active against fungal strains. The results were compare...
Yang Liu1, Jing-xin Mao2, Xiao-dong Wei2, Man Yi2, Xiao-long Zhang2, Ke Zheng3, Xian-xin Chen4, Guo-Ze Wang5 and Bing-bo Chen1,*
...crobiota, promoting the digestion and absorption of nutrients, which significantly improved weight gain rate and the quality of external fur. It was suggested that BFA has a positive and effective effect on improving the nutrition of rats and promoting their healthy growth.
...
Catherine J. Allen
...ince the early 2000s, indigenous notions of well-being (Quechua sumak kawsay, Aymara suma qamaña, Spanish buen vivir, vivir bien) have entered the political discourse of the Andean nations (Bolivia, Ecuador, Peru), serving as rallying points for networks of indigenous federations, environmentalists, NGOs and intellectuals united in a critique of neoliberal development paradigms. Ecuador and B...
James Maffie
...stood in terms of the indigenous concept of a nepantla process, a paradigmatic example of which was the artisanal process of weaving. Humans accordingly attained balance: first, by weaving together individual behavioral extremes (such as fasting and feasting) into a well-middled, individual life fabric; and second, by weaving themselves together with other human and nonhuman agents into a single, well-middled, community life fabric by means of initiatin...
Brian Yazzie Burkhart
...mpts to articulate an Indigenous concept of well-being as grounded in the concepts of being-from-the-land and being-in-the-land, which are attempts, in this essay, at describing a conceptualization of being in general and human being in particular that is both out in the land and at the same time arising from it. It is from this perspective of being that this essay tries to articulate a framework for understanding well-being that is both rooted in the land and...
Luo Lei1, Xingxing Deng1, Dengyue Yuan2, Zonglin Zheng1, Chengke Zhu1, Hui Luo1, Baohai Li3, Hua Ye1,* and Chaowei Zhou1,3,*
...hat npy is an orexigenic peptide in gibel carp. In conclusion, these results indicated that npy is a conserved peptide within vertebrates and might exert orexigenic role in gibel carp.
...

John Ogunsola1*, Richard E. Antia2, Benjamin O. Emikpe2 and Olajumoke A. Morenikeji

Jesse Butler
...uch commonality among indigenous worldviews and their capacity to address our collective ecological well-being together on planet Earth. Specifically, I will argue that contemporary philosophers Viola Cordova and Tu Weiming exhibit ecological self-understanding as an epistemic virtue in their conceptions of human existence. Ecological self-understanding (ESU) is veridical acknowledgement of human beings as embodied agents in the world, fundamentally situated w...

 Adile Tatliyer1,*, Sinan Bas1 and Serdar Yagci2

 

...h period, factors whose eigenvalues which is the characteristic roots of a square matrix with p×p were higher than 1 were elected by applying the Varimax rotation in explanatory factor analysis. In the first period of average 11 days after birth, 2 factor scores (FS) were used as new latent predictors in order to predict live body weight in multiple linear regression model. The 2FS at the first period, 3 FS at the second period, 4 FS a...

Saqib Bashir* and Bashir Ahmad 

...o observe the effect of pigeon pea green manuring on performance and subsequent nitrogen fertilizer requirement of wheat crop during 2013 and 2014. The experiment was carried out in randomized complete block design with split plot arrangements having four replications. Pigeon pea green manuring treatments as 0, 30, 60, 90 and 120 days post emergence were allotted to main plots, where crop was incorporated in soil at differen...

 Sohaib Afzaal1, Usman Hameed2, Nasir Ahmad3,*, Naeem Rashid3 and Muhammad Saleem Haider1

...ties and good quality indigenous starter cultures from them are anticipated to be employed in food industry.

...

Bata Shalangwa Ishaku1, Batim Turdam1, Maimadu Abdullahi1, Waziri Ibrahim Anjili2 and Mayowa Olabode2 

...au State, North Central Nigeria. Fecal and blood samples were collected from 300 donkeys and analyzed using a standard laboratory procedure. Coprological examination conducted using flotation and sedimentation techniques showed that 276 (92.0%) were positive for gastrointestinal nematodes. A greater proportion (70.7%) of the nematodes were Strongyles followed by mixed infection of Strongyles and Parasacaris equorum (15.2%) and Strongyloides westeri (14 1%). Ch...
Faiz-ul Hassan1,*, Muhammad Sajjad Khan1, Muhammad Saif-ur-Rehman1, Syed Muhammad Raihan Dilshad2 and Muhammad Amjad Ali3
...ose of four Pakistani indigenous breeds (Morna, Saen, Kakka Bralanwala and Anmol) from local horse breeders of Punjab province. Genomic DNA was extracted from the blood samples and amplified by PCR using 40 RAPD primers. PCR products were separated on an agarose gel electrophoresis and scoring of bands was performed. Phylogenetic tree was constructed by UPGMA method to determine the genetic similarity among the horse breeds. Thoroughbred and Arab horse breeds ...

 Ping Zhang1,2, Yabin Pu1, Yu Zhang2, Jia Chen2, Kunfu Wang3, Qian Li3, Yujiao Sun3, Yuehui Ma1, Shuqing Jiao2,* and Weijun Guan1,*

...sheep, and the stepwise digestion by XI collagenase and trypsin was used to isolate MDSCs and were purified by differential attachment technique. Cell morphology were observed using an inverted microscope, and MDSCs proliferation pattern were determined through growth curve analyses. MDSCs were identified by immunofluorescence and RT-PCR, and the immunofluorescence antibody invoved Sca-1, CD34, CD144, Desmin and CD45 which are the makers of MDSCs. Finally, MDS...
Fukuan Du1,2, Yongkai Tang1, Juhua Yu1, Shengyan Su1, Fan Yu1, Jianlin Li1, Hongxia Li1, Meiyao Wang1 and Pao Xu1,2,*
...m. We obtained 123,764 unigenes with an average length of 1092 bp and a total of 115,169 putative Simple Sequence Repeats (SSRs). Among them, 37 SSRs were selected for the validation experiments. All of the loci were found to be polymorphic and showed bi-allelic in 60 individuals of C. nasus. These SSR markers should not only be useful for population conservation, but also for construction of genetic linkage map and economic performance improvement of <...

Muhammad Sohail1*, Asad Sultan2, Said Sajjad Ali Shah3, Muhammad Sajid1 and Adnan Khan4

...cted to investigate the digestibility of cereal grains like wheat, maize, sorghum and their cultivars in quail birds. A total of 270 quail birds were raised in cages having three main groups, one each for wheat (W), maize (M), sorghum (S) and six subgroups for the collection of fecal samples and further analyses. Nutrient profile, digestibility coefficients, and apparent metabolizable energy were investigated. Fat percentage...
Yuyu Wang, Gangchun Xu*, Zhijuan Nie, Quanjie Li, Nailin Shao and Pao Xu*
...biochemical parameters, digestive enzymes activity and antioxidant status of largemouth bass (Micropterus salmoides) reared in an in-pond raceway system (IPRS). Fish (initial average body weight: 35.68±2.12g) were randomly allotted to in-pond raceways (26.2m×5m×2.5m) stocked at two stocking densities (68 and 114 fish/m3, respectively). Fish were fed twice daily (08:00 and 17:00), and the daily ration feed was 4% of the body...
Lichao Feng1,2, Shaoqing Zhang4, Dianyuan Chen2, Sina Adl3 and Donghui Wu1,4*
... nectar of flowers (Erigeron annuus, Trifolium repens, Heteropappus hispidus, Potentilla chinensis, Hibiscus trionum), or leaves of grasses (Poa annua, Echinochloa crusgalli), or maize leaves (Songyu 419, a hybrid variety) as food sources to feed the adult of A. fuscicollis. The larvae were reared on roots of the same grasses and germinated maize seedlings. Additionally, potato tuber the common ...
Xiaona Cao1,3-5, Yuanyuan Ren2-5, Xiaoteng Cui2-5, Baoxin Qian2-5, Chunyan Zhao 2-5, Jie Yang2-5, Chao Su2-5,* and Xingjie Gao2-5,*
...ein A1 (hnRNP A1), Hu Antigen R (HuR) and T cell intracellular antigen 1 (TIA1), in the SG aggregation and nucleus/cytoplasm localization under stress condition. We found that hnRNP A1, HuR and TIA1-containing SGs were aggregated in the cytoplasm of HeLa cells, and accompanies the alteration of nucleus/cytoplasm localization during arsenite induced-oxidative stress. Increased hnRNP A1 fluorescence signal within cytoplasm was...
Songhao Zhang1, Yiwen Gong1, Jian Xu1, Mou Hu2, Peng Xu3 and Yanliang Jiang1,2,4,*
...iles showed that 2,767 unigenes were significantly differentially expressed, including 1,663 genes up-regulated and 1,806 down-regulated in purse red carp. Moreover, gene pathway analysis of the differentially expressed genes was conducted. We proposed the skin color and body shape depended upon at least four pathways including melanin biosynthesis, TGF-beta signaling, Wnt signaling and MAPK signaling pathways. We obtained candidate genes that may affect the m...
Hong Ma*, Bo Fu, Liang Wang, Zhong-qiu Li and Di Liu*

 

...and can be modified by epigenetics. However, whether HSPC117 affects development of cloned embryos mRNA expression is unknown. To investigate the influences of HSPC117 on embryonic development, we generated transgenic porcine embryos by handmade cloning. We then assessed the embryonic developmental rate at cleavage and blastocyst stages. Our results showed that the HSPC117 transgenic embryos had markedly higher cleavage and blastocyst rate...
Muhammad Afzal1, Nighat Sultana1, Ali Hassan1, Syed Zakir Hussain Shah2,*, Mahroze Fatima3, Syed Makhdoom Hussain4, Muhammad Bilal5 and Majid Hussain2
...omposition and nutrient digestibility performance by rohu (Labeo rohita) juveniles fed citric acid (CA) acidified phytase (PHY) pretreated soybean meal (SBM) based diet.Basal SBM based diet was supplemented with two levels of CA (0 and 2%) and two levels of microbial PHY (0 and1000 FTUkg-1) in factorial arrangement resulting in the formulation of four experimental diets. Chromic oxide (1%) was added as an inert marker in the diets to evaluate...

Tahira Jatt1, Ghulam Sarwar Markhand1, Lane Rayburn2, Ray Ming3, Mushtaque Ahmed Jatoi1 and Ameer Ahmed Mirbahar1,4* 

...type one of the seven indigenous commercial date palm varieties and four wild type date palm of Sindh province, Pakistan by using the traditional Fuelgen squash method. Results indicated that all seven commercial varieties and four wild types of date palm are diploid in nature and having 2n=36 chromosomes. First time detailed karyotype of these varieties have been developed which includes total chromosome length (TCL), relative length (RL) and centromeric inde...
Hafiz Abdul Ghafoor*, Muhammad Afzal, Muhammad Asam Riaz and Muhammad Zeeshan Majeed
...sential oils of eight indigenous plant species for their insecticidal potential against 2nd instar D. mangiferae individuals. Standard twig-dip method was used for toxicity bioassays according to Completely Randomized Design. Mortality of mealybug individuals varied with plant materials and increased along with the extract concentration and exposure time. Botanical extracts of Azadirachta indica (neem) and Gardenia jasminoides (...

Hala Mohamed Nabil Tolba1, Naglaa Fathy Saeed Awad1*, Gamelat Kotb Farag Kotb2, Amany Adel3 

...ces in the predictive antigenic determinants between these vvIBDV isolates and the classic vaccine strain (Bursin plus). These findings could explain the persistence of vvIBDV circulation in the Egyptian environment in spite of vaccination with classical vaccine strains.  

...
Mohammed Anas Chowdhury1, Md. Abdul Karim1, Md. Tayfur Rahman1, Shoaibe Hossain Talukder Shefat1, Ashikur Rahman1, Mohammad Amzad Hossain2*
Mohsen M. El-Sherbiny1,2,*, Reny P. Devassy1, Erinn M. Muller3, Abdulmohsin A. Al-Sofyani1 and Ali M. Al-Aidaroos1
...r column (Acartia negligens, Clausocalanus furcatus, Lucicutia flavicornis, Pleuromamma indica and Haloptilus longicornis).
...
Lusha Liu1,*, Lei Cheng2, Xingqi Zhang3, Yulong Li1 and Jianping Jiang1,*
...e identified in 15,385 unigenes which were generated by Illumina paired-end sequencing in M. fissipes. Within all microsatellites, AG/CT, AAG/CTT, and AAAG/CTTT are most prevalent motif in each repeat class. We randomly selected 61 unigenes with the microsatellite to design primers and do genetic analysis in the Sichuan basin population of M. fissipes. Of all, 35 primer pairs (57.38%) successfully amplificated ...

Samina Qamer1, Farkhanda Asad1*, Amna Faiz1, Robina Arshad1, Zunaira Shaheen1 and Tahira Yasmin2 

...rganic chromium on feed digestibility and muscle composition of (Labeo rohita fish). Six treatment groups were prepared with one control group (each with two replicates), three groups supplemented with inorganic chromium chloride hexa hydrate (CrCl36H2O by 0.3, 0.5 and 0.6mg/kg) and three groups supplemented with organic chromium picolinate (Cr-Pic-0.3, 0.5 and 0.6mg/kg) were used. It was found that Labeo rohita fed on inorganic chromium (CrCl2 Hexahydrate) sh...
Syed Makhdoom Hussain1*, Nisar Ahmad1, Azhar Rasul1, Muhammad Mudassar Shahzad2, Muhammad Latif3, Muhammad Zubair Ul Hassan Arsalan1, Muhammad Umair1  and Hafiza Hina Shafqat1
...h performance, nutrient digestibility and hematological parameters of C. catla fingerlings fed nano- Cr particles supplemented sunflower meal based diets. The experiment was consisted on seven test diets on the basis of supplementation of nano Cr graded levels (0, 0.5, 1, 1.5, 2, 2.5 and 3 mg Kg-1). Chromic oxide was used as an inert marker. Fingerlings were fed at the rate of 5% of their live wet weight. Maximum improvement in the growth per...
Wen-Feng Li1, Rong-Yue Zhang1, Chun-Hua Pu2, Jiong Yin1, Zhi-Ming Luo1, Xiao-Yan Wang1, Xiao-Yan Cang1, Hong-Li Shan1 and Ying-Kun Huang1,*
...ey on Ceratovacuna lanigera Zehntner; and Euborellia pallipes Shiraki, Orius (Heterorius) minutus (Linnaeus) and Scymnus (Neopullus) hoffmanni Weise which preys on Saccharicoccus sacchari (Cocherell), Baliothrips serratus Kobusand Trochorhopalus humeralis Chevrolat. Protecting these natural enemies will encourage natural control of pest species while protecting the environment and mai...
Naheed Ikram and Nafisa Shoaib*
...of fungi Aspergillus niger, Aspergillus flavus, Rhizopus stolonifer, Penicillium sp. and Fusarium sp. from 14 different genera of commercially important marine fishes Acanthopagrus sp. Parastromateus niger, Nemipterus sp., Pampus argenteusIlisha sp., Alepes djedabaEpinephelus sp., Teraponjarbua, Terapon puta, Scomberomorus korean...
Zil-e-Huma1, 2, Abdul Malik Tareen3, Kaleem Ullah3, Tauseef M Asmat4,Abdul Samad4Asim Iqbal2, Mohammad Zahid Mustafa3*,Irshad Ahmad5 and Sadeeq ur Rahman6
...oli (EAEC), enterotoxigenic E. coli (ETEC), enteroinvasive E. coli (EIEC), and enterohemorrhagic E. coli (EHEC). Results show that the virulent EAEC strain of E. coli was found to be the most prevalent (35%), while EPEC was identified in 15%, followed by EHEC (8%). Notably, none of the samples was found positive for EIEC. Moreover, ETEC was identified in 11% cases of co-infection. Results showed that children with less than two ...
Ghaleb Adwan*, Sami Bdir and Kamel Adwan
... intermediate hosts for digenetic trematode cercariae. Digenean larvae were obtained from M. praemorsa snails that were collected from Palestine. Cercaria melanopsi palestinia I and III were identified as a xiphidiocercaria belonging to the microcotylae sub-group and as a microcercous cercaria, respectively. Phylogenetic analyses based on sequences of the ITS2 region of the nuclear rDNA revealed that C. mela...

Zubaria Malik, Zahir Shah* and Muhammad Tariq 

...esults suggested that indigenous AMF can be increased in soil and roots of wheat and maize with biochar amendment in an alkaline calcareous soil with low fertility. 

...
Zakirullah1, Hamayun Khan1, Ali Gohar2, Haq Amanullah1,*, Muhammad Younas1, Muhammad Tarique Tunio3, Muhammad Saleem Khan4, Ibrar Muhammad Khan1 and Noushad Khan5 
...lls, three Achai (the indigenous breed) and three Holstein Friesian (exotic breed) amount to the experimental material at Government Cattle Breeding and Dairy Farm Harichand, Charsadda. Separate artificial vagina was utilized for semen collection from either breed, maintained at 42°C temperature. Gross and microscopic examination was carried out subsequent to the semen collection. Semen specimen with more than 70% motile characteristics were used for furth...
Abd El-Nasser Ahmed Mohammed1,2,*
...n of 1.0 and 2.0% of Nigella sativa (N. sativa) oil to female mice and secondly to explore if dietary N. sativa is effective in alleviation of hypothermia and hyperglycemia due to general anesthesia. Seventy-five albino female mice (21.74 ± 0.19) were distributed into three groups; control (G1; N=25; not receive N. sativa oil) and two N. sativa oil groups supplemented with either 1.0% (G2; N=25) or 2.0% (G3; N=25) for ...
Hafiza Mehwish Iqbal1, Shahid Yousaf1, Salman Khurshid1*, Qurrat Ul Ain Akbar1, Saqib Arif1, Nasreen Fatima2 and Ali Murad Rahoo3
...ies viz. Aspergillus niger, Aspergillus flavus, Fusarium oxysporum, Rhizopus stolonifer and Alternaria alternata have been identified in citrus samples. However, major incidence was recorded for Aspergillus niger (41.6%) followed by Fusarium oxysporum (27.7%). Infected samples (5.9) had high pH as compared to the healthy samples (3.1). Total soluble solids were decreased from 14 to 8.5% due to fun...
Adel E.M. Mahmoud1, AdamCieślak2, Paulina Szulc2, Anna Łukomska3 and Małgorzata Szumacher-Strabel2,*
...rs including dry matter digestibility.
...
Gul Afshan1,2,*, Soumble Zulfiqar2, Sumaira Mehboob2, Muhammad Tahir Javed Khan1 and Abdul Rauf Shakoori2,*
...ntibodies against the antigenic protein HCV3a E2, the purified protein E2 was injected subcutaneously into the rabbit (Oryctolagus cuniculus). The antibodies titer in rabbit after immunization in 100% pure serum sample was 0.736 which was 15 times more than that of pre immune control sera (0.049). It is concluded from the current study that purified HCV3a envelope glycoprotein E2 has significant antigenic activ...
Chen-Yu Sun, Wen-Chao Liu*, Mei Xiao, Zhi-Hui Zhao and Li-Long An*
...morphology in Chinese indigenous young yellow feather broilers. A total of 360 male, one-d-old Huaixiang chickens with an average initial BW of 44.52 ± 1.07 g were randomly allotted to 4 treatments. Each dietary treatment consisted of 6 replicate cages, with 15 birds per replicate. The 4 dietary treatments were corn-soybean meal-based diets and supplemented with 0, 500, 1000 and 2000 mg/kg betaine, respectively. During d 1-14, with increasing the dietar...

Matiullah Khan*, Shameemul Sibtain Shah, Atiqullah Khan, Muhammad Sarwar and Shoaib Ahmed 

...mula “100 kg of indigenous HRP with total P2O5 content of X and mesh size 160 when treated with 60 liters (50% diluted) H2SO4 (volume/volume), followed by proper curing, yields SSP of almost X/2 % extractable P2O5”. The P was applied @ 57.5 kg P2O5 ha-1 both from prepared SSP and DAP to wheat crop on equal P2O5 basis. Also, Phosphate Solubilizing Bacteria (PSB) were tested in this experiment for yield and P uptake of the crop. The results showed th...
Uzma Rafi*, Asmat, Muqaddes Niaz, Syeda Shazia Bokhari and Aisha Waheed Qurashi*
...se) and IM2 (Alkaligenes faecalis) were isolated from external surface and rectum portion of cockroach, respectively. Bacterial autoaggregation started increasing after 1 h of culture incubation and reached maximum after 4 h. Isolate CXA1 showed maximum (136 %) auto aggregation as compared to isolate IM2 (37 %) after 4 h. However, biofilm formation in the isolate IM2 was higher as compared to isolate CXA1 at non shaking conditions. The  study s...
Muhammad Suleman1,*, Abu ul Hassan Faiz2, Muhammad Shahbaz2 and Ayesha Riaz3
...ation of Aspergillus niger at different concentrations of wheat bran as carbon source (2.5, 3.0, and 3.5 %) on the production of xylanase. Submerged fermentation was carried out in 500 ml Erlenmeyer flasks using vogel’s medium at 35°C for five days. UV irradiated A. niger has significantly increased the xylanase production as compared to parent strains. Twenty minutes exposure of A. n
Zia-ud-Din1, Muhammad Nauman Aftab2, Tanveer Hussain3,*, Irfana Iqbal4 and Asma Zafar5
...uch flocks.
...
Sabbah M. Allam, T.M. El-Bedawy, M.H. Bakr and A.E.M. Mahmoud*
...dairy cows, on nutrient digestibility, blood constituents, level of antioxidants in plasma, and presence and count of fecal pathogenic bacteria e.g. E. coli and Salmonella spp. Forty lactating Holstein cows, weighing 550±50 kg, with an average of 20 kg daily milk yield, were randomly divided into four groups (ten in each group). Animals were fed rations containing DOP at 0, 25, 50 and 75% substitution of yellow corn grains (R1, R2, ...
Muhammad Mudassar Shahzad1*, Syed Makhdoom Hussain2, Afia Muhammad Akram1, Arshad Javid3, Majid Hussain5, Syed Zakir Hussain Shah4 and Asma Chaudhary1
... on growth and nutrient digestibility of the Catla catla fingerlings fed Moringa oleifera leaf meal (MOLM) based diet. The phytic acid’s presence in plant by-products decreases the bioavailability of nutrients to fish, resulting in poor fish growth and low nutrient digestibility in the body. Experimental diet was divided into six groups and were supplemented with graded levels (0, 300, 600, 900, 12...
Muhammad Usman Akhtar1,2, Abdul Qayum3, Anshan Shan1,*, Shuli Chou1, Hyeonsoo Jo4, Syed Waqas Ali Shah4 and Ishfaq Muhammad5
...trient intake, nutrient digestibility, nitrogen balance, blood urea nitrogen (BUN) and milk yield of lactating Beetal goats. Thirty five lactating Beetal goats were randomly divided into five groups (seven goats per group) in a randomized complete block design. Five isocaloric and isonitrogenous diets were formulated to contain 30, 40, 50, 60 and 70% RDP of dietary protein represented as 30RDP, 40RDP, 50RDP, 60RDP and 70RDP, respectively. Feed intake was recor...

Faleye Temitope Oluwasegun Cephas1,2*, Adewumi Moses Olubusuyi1, Olayinka Olaitan Titilola3 and Adeniji Johnson Adekunle1,3 

...ldren (<15 years) in Nigeria with acute flaccid paralysis (AFP). All CCS were screened for Enteric Adenoviruses (EAVs) and group A Rotavirus (GARV) using a rapid immunochromatographic test kit. Subsequently, they were passaged in HEp-2 cell line. All isolates were subjected to RNA extraction, cDNA synthesis, three (5l-UTR, VP1 and EV Species C [EV-C]) different PCR assays and sequencing of amplicons. EVs were further subjected to [‘]Illumina sequencin...

Adewumi Moses Olubusuyi1, Ogunsakin Temitope Rachael1, Ogunrombi Sefiu Babatunde1, Ojeamiren Iduda1, Olawole Alaba Samuel1, Faleye Temitope Oluwasegun Cephas1,2* and Adeniji Johnson Adekunle1,3 

...surveillance program in Nigeria and declared negative for enteroviruses because they did not produce CPE in RD and L20B cell lines. In all, 120 cell culture supernatants (60 each on RD and L20B cell lines) that emanated from 30 stool suspensions (2 samples per case) were analyzed as 60 pools (pooled per case by cell line). Pools were subjected to RNA extraction, RT-snPCR, amplicon sequencing and phylogenetic analysis. Eleven and one of the 30 pools of RD and L...
Tasleem Akhtar1,2,Ghazanfar Ali1,*, Nuzhat Shafi2 and Abdul Rauf2
... S. plagiostomus, S. niger and S. esocinus, respectively. Haplotype diversity was lowest and same in S. esocinus, S. niger and S. progastus (0.0001) and highest in S. plagiostomus (0.5436). Average number of nucleotide differences of 4 species (Kt) was 0.737 and total nucleotide diversity (Pi) was equal to 0.0004 ± 0.0001. Out of 1552 sites, 1547 sites were found to be conserved and ...
Zhijun Zhong1, Rui Tu1, Xichun Wang2, Yi Geng1, Qicheng Xiao1, Yinan Tian1, Bin Wei1, Jiaming Dan1, Ya Wang1 and Guangneng Peng1,*
...on of B. canis antigens in the lesions of all examined tissues. Strong positive staining was primarily found in the spleen, liver, and testicle. In conclusion, this study was the first to report the isolation of two B. canis strains from pet dogs in Sichuan province, southwestern China, and to further evaluate B. canis antigen location in tissues. Our study will contribute to the understanding of B. c...
Muhammad Nisar* and Muhammad Idress
...onsideration’s negligence in development agendas. Home gardening data provide complete agricultural input in a nation’s domestic treasure. Therefore, the current study was carried out with the objective to assess small farmers’ involvement in home gardening at district Tank, Khyber Pakhtunkhwa- Pakistan. Three hundred and twenty (320) small farmers were selected randomly on the basis of their involvement in home gardening via a pre-tested clo...

Muhammad Irfan Ullah1*, Muhammad Arshad1, Muhammad Imran Khan1, Muhammad Afzal1, Azhar Abbas Khan2, Syed Muhammad Ali Zahid1, Muhammad Saqib1, Asad Abdullah1, Saba Kousar1 and Maryam Riaz1 

...bollworm, Helicoverpa armigera (Hub.) (Lepidoptera: Noctuidae) is a major insect pest in cotton crop and is being the most noxious one in cotton growing areas of the world. Knowledge about how water stress modifies the ability of plants to resist against insect feeding is an interesting and important component in an integrated pest management program. In the present study, water stress was applied to field grown cotton with two Bacillus thuringiensis transgene...

Muhammad Shahzad Akbar*, Maria Aslam, Muhammad Rehan Khalid, Shahid Iqbal, Muhammad Luqman and Muhammad Zeeshan Majeed 

Ahmad Ali1,2,* and Aleem Ahmed Khan1
...n biological traps were pigeon (Columba livia), quail (Coturnix coturnix), sparrow (Passer domesticus), myna (Acridotheres tristis), dove (Streptopelia senegalensis) and mice (Mus musculus). While the percentage use of quill, mice, dove, sparrow and myna has been calculated as 66.67%, 46.24%, 46.24%, 34.41% and 21.51%, respectively. Further it was observed that falcon trapping ...
Memis Ozdemir
...RsaI polymorphic digestion site. As a result of the study, it has been seen that the Prl/RsaI specificpolymorphic site is on exon 4 in Bos taurus cattle breeds, but have been not detected the RsaI restriction enzyme digestion site in Prl gene exon 3 region reported by many literature and, these results also have been approved by blasted at the NCBI Genbank.
...

Muneer Abbas1*, Muhammad Ramzan1, Niaz Hussain1, Abdul Ghaffar1, Khalid Hussain1, Sohail Abbas2 and Ali Raza3 

...l agents. Helicoverpa armigera, Spodoptera litura, Agrotis Sp., Bemesia tabaci and Microtermes Spp. were major pests of gram and mungbean attracted in light traps. May, June and July were hottest months of the year with highest population captures of 2892, 2789 and 2475, respectively. Temperature had significant impact of 80.7 % on per unit population attraction (r=0.807). However, humidity had no significant impact (2.9 %) on per unit population attraction in...

Hala Rajab1, Muhammad Sayyar Khan1*, Safdar Hussain Shah1 and Syed Mehar Ali Shah2 

...ation of shoots on Murashige and Skoog (MS) media containing BAP 3 mgL-1 and developed roots in ½ MS media supplemented with IBA 3 mgL-1 in case of OSCAR cultivar. Successful selection of transgenic lines was achieved using selective antibiotic kanamycin 50 mgL-1. NtSAT4 gene integration into the plants genome was confirmed by PCR using the NtSAT4 gene specific primers. The positive transgenic lines were successfully acclimatized to soil and glass hous...

Muhammad Naeem Khan* and Asad Jan 

...za, head ach, liver and digestive problems and healing of wounds. The aim of the study was to explore phytochemical constituents of methanolic crude extract (MCE) and various solvent fractions and in-vitro antimicrobial activities of whole plant of D. botrys. Qualitative phytochemical screening of MCE and solvent fractions of D. botrys showed the presence of alkaloids, phenols, flavonoids, saponins, tannins and sterols, however in n-hexane fraction (HxF) only ...
Muhammad Furqan Shahid1*, Tahir Yaqub1, Muhammad Yasin Tipu2, Asim Aslam2, Saima Yaqub1, Aziz-ul-Rahman3, Muzaffar Ali1
...te the infectious and antigenic potential of three different AIV (A/H9N2) variants isolated from commercial, backyard poultry and fancy birds (partridge). Commercial broiler birds were infected experimentally at the dose of 106 EID50 by using these three isolates. Results revealed that onset of clinical signs was started at 2nd day post infection (DPI) followed by severity in redness and swelling of eyes, sneezing and lacrimati...
Fizza Anwar1, Muddassar Zafar1*, Zahid Anwar1, Raja Tahir Mehmood2
...fer, Aspergillus niger, Aspergillus fumigatus and Aspergillus terreus were tested for the production of antimicrobial agents. The produced antimicrobial metabolites included Aflatrem, Monacolin K, Fusarubin and Tautomycetin. Different fermentation parameters were optimized to produce antimicrobial agents using Response Surface Methodology (RSM) and best results were obtained at 45°C temperature, 5.5 pH, 5 mL inoculum con...
Hafiza Sadaf Zahra1, Asia Iqbal2, Sayyeda Hira Hassan1, Hafiz Abdullah Shakir1*, Muhammad Khan1*, Muhammad Irfan3, Chaman Ara1, Shaukat Ali4
...ch results in abnormal epigenetic changes that relates with an increased risk of BC. The most important ALAN-mediated epigenetic changes include methylation of DNA and acetylation of histone, which are significant for growth, development and progression of BC. DNA hypermethylation of promoter CpG islands inhibits transcriptional activity by methyltransferase enzyme which results in inactivation of tumor suppressor genes (TSG...

Imran1*, Amanullah1, Muhammad Arif1, Zahir Shah2 and Abdul Bari3 

...us L., IPomoea nil L., Erigeron canadensis L., Echinochloa crus-galli L., Asparagus officinalis L., Cynodon dactylon L., Digera muricata L., Cyprus rotundus L. and Solanum nigrum L. The most abundant and flourished weeds were Cyprus rotundus L, and Cynodon dactylon. Results revealed that peach organic sources (biochar) and Trichoderma drastically reduced weeds frequency, weeds biomass at flowering, pods formation and physiol...

Sana Munir1, Muhammad Kamran Qureshi1*, Ahmad Naeem Shahzad2, Ismat Nawaz3, Muhammad Shahzad Anjam4, Sumaira Rasul4 and Muhammad Asif Zulfiqar5 

...ut of nineteen PC, with eigen value >1, contributed about 80.326 % of total variability. Positive contribution towards PC1 was given by seed traits such as lint/seed, seed weight/seed, boll weight, seed index and seed cotton/seed. In biplot analysis, intraspecific hybrids and their parents were present very close to each other while interspecific hybrids, their parent and exotic line were unsimilar and far apart from intraspecific hybrids. Results of linkag...
Barkat Ali1,2, Wasim Sajjad1,2,3, Imran Khan1, Muhammad Rafiq1, Sahib Zada1, Aamer Ali Shah1 and Fariha Hasan1,*
...by strains like; Alcaligenes spp. N14 and N21, Bordetella spp. N30 and Streptomyces spp. N28. Alcaligenes spp. N27 and Lysinibacillus spp. P17 showed strong activity against all the three ATCC strains. The study concludes that the bacteria isolated from the rocks having substantial resistance to heavy metals are also showing good antibacterial activity as well as, and they are also potentia...

Iffat Nawaz1*, Farhatullah1, Fida Muhammad1, Sajid Ali2 and Ghulam Muhammad Ali

...ersity. A total of 96 indigenous accessions of common beans (Phaseolus vulgaris L.) were collected from three different areas of Himalaya region of Pakistan. In all accessions, 78.1% were collected from Khyber Pakhtunkhwa while 11.5% from Kashmir and 10.4% from Gilgit Baltistan. Primary evaluation of seed characteristics was done according to International Board of Plant Genetic Resources (IBPGR) descriptors. Significant variation was observed for seed color, ...

Ghulam Hussain1, Muhammad Asrar1, Dilbar Hussain2, Khurum Zia3, Abdul Rashid4*, Hina Anwar5, Muhammad Azeem1, Saddam Hussain1 and Sabeen Asghar1 

...ilable pesticides and indigenously available plant extracts. The efficacy of following insecticides and plant extracts viz. Cascabela thevetia leaf extract @ 300ml/acre, Azadirachta indica @ 300ml/acre, Profenofos @600ml/acre., Crown 70WS @125gm/acre, Match 50EC@250ml/acre, Helmat 40EC@600ml/acre and Confidor 20SL@ 250ml/ acre were evaluated on cotton against 3rd instar cotton mealy bug. Data was collected after 3,6,12,24 and 48 hours. Maximum mortality of cot...
Tanweer Kumar1, Sahib Zada2,3,Muhammad Irfan4,*, Huma Batool5 and Wasim Sajjad2,6
...o hepatitis B surface antigen Immuno-chromatographic test (ICT), HBsAg enzyme-linked immunosorbent assay and by Reverse transcription polymerase chain reaction for the existence of antibodies against HBV. It was observed that 307 (12.8%) out of the 2382 individuals harbored antibodies in their blood against HBV. Among the different age groups, the highest number of incidences of HBV antibodies was found in the 16-25 age groups (7.01%). ICT positive samples wer...
Nazir Ahmad Khan1,*, Mudassir Alam1, Rafiullah khan1, Kamran Khan2 and Sadeeq ur Rahman3
...quo;s performance, diet digestibility, nitrogen retention, meat amino acids profile and farm profitability. Grains of QPM200 and QPM300 hybrids, approved by Pakistan maize variety evaluation committee in January 2017, were provided by International Maize and Wheat Improvement Centre (CIMMYT), Pakistan. The content of CP was higher in QPM300 (10.8% dry matter (DM)) and QPM200 (9.12% DM) than the CM (7.78% DM). Moreover, QPM200 and QPM300 had higher contents (% ...
Mubasher Ahmad Malik1, Samina Jam Nazeer Ahmad2, Muhammad Jalal Arif1 and Jam Nazeer Ahmad1,*
Mustafa Koyun1*and Şenol Çelik2
...d D. spathaceum (digenetic trematode) on the weight and sex of the host fish (Mastacembelus mastacembelus) by using some data mining algorithms. During this study, the fish weight (g) and length (mm) of 122 fish were measured. In addition, the distribution of M. heteranchorus and U. pictorum in each lamella of the gills and the distribution of D. spathaceum in the right and left eye lenses were evaluated. Two different algori...

Naglaa M. Hagag1*, Mervat E. Hamdy1, Mary A. Sargious1, Sara M. Elnomrosy1, Nahed A. Ahmed1, Ayman A. Hamed1, Ahmed R. Habashi1, Essam I. Ibrahiem1, Mahmoud A. Abdel-Hakim2 and Momtaz A. Shahein

...t. Moreover, the high antigenic variation in FMDV is recognized as a major problem in vaccination. Because the vaccine strains should match those strains circulating in the field, an updated vaccine is required to control the disease. Monitoring of FMDV in neighboring countries is essential to predict those strains that might escape into Egypt.  

...

Bamigboye Olutoyin Omolara1, Fapohunda Funmilayo Omotoyosi1, Oladipo Elijah Kolawole1,2, Oni Mattthew Olujenyo1, Ajibade Oluwatosin Akinola1, Kaka Mary Oluwatosin1* 

Farheen Shafique1, Shaukat Ali2,*, Saiqa Andleeb1, Abdul Rauf1,3, Syed Ayaz Kazmi1,3, Sadia Idrees4, Faisal Farooq1,3, Faiq Nawaz Khan3,4, Muhammad Saad-ul-Hassan1,3, Raja Awais Mumtaz1,3, Saba Khalid1, Zaheem Ashraf1, Hafiz Muhammad Tahir2 and Fazal-ur-Rehman5

...h hepatitis B surface Antigen (HBsAg) and Anti hepatitis C antibody (Anti-HCV antibody) after initial screening with no coinfection of both diseases. Out of 25 total infected individuals, 05(1.6%) were found HBsAg positive and 20(6.6%) were found anti-HCV positive. All the ICT positive individuals were further confirmed by quantitative Enzyme Linked Immunosorbent Assay (ELISA) and 23(7.6%) individuals were confirmed for both hepatitis B and C including 05(1.6%...
R. Yasmeen1*, S. Shaheen1, B.N. Khan2, S.S. Bokhari1, U. Rafi1 and A.W. Qurashi1
...Samples were chemically digested and finally analysed by Flame Atomic Absorption Spectrometery (FAAS) for four heavy metals such as Pb, Cd, Zn and Cu. The results showed all the metals were in safe range in feed samples, a very negligible amount was present in water samples and small concentrations were noticed in soil samples. The amounts of Zn and Cu in feed were reflected in fecal samples; however, higher amount of Pb and Cd were noticed in fecal sam...

Hamoudi Naoual1, Alloui Nadir2*, Barberis Abdelhak2 and Boudaoud Amine2 

...nas strepera), Eurasian wigeon (Anas penelope) and Common shelduck (Tadorna tadorna). Our observations confirm the diversity of migratory bird species, particularly Anatidae infected with AIV, in the wetlands of Eastern Algeria. Molecular characterization of circulating avian influenza viruses in these wild birds will help assess the potential for spread of these viruses in poultry farms. 

...
Erum Yawar Iqbal1*, Ashfaque Ahmed Nahiyoon1, Shahnaz Dawar2 and Shahina Fayyaz1
...olecularly identified indigenous entomopathogenic bacteria (EPB) Xenorhabdus have been used to access their efficiency against cotton Aphid Aphis gossypii (Glov.). An efficient formulation of different parts of bacterial culture such as, cell-free supernatant, crude cell extract, bacterial culture and methanol extract in in vitro and green house condition have been exploited. The results have shown that different formulants of EPB produced...
Nafeesa Batool1*, Javed Iqbal Qazi1, Nida Aziz1, Ali Hussain2 and Syed Zawwar Hussain Shah3
... countries by anaerobic digestion (AD) and co-digestion (Co-AD) under mesophilic (35 ± 2 ﹎) settings at substrate to microorganism (S / M) ratio of 1.0 based on volatile solids (VS) for 30 days. Solids [Total solids (TS), volatile solids (VS), fixed solids (FS) and their ratios as (VS / TS)] determinations were also carried out for process monitoring. The highest cumulative biogas yield of 459.4 mL /g VS was observe...
Peng Peng1,3, Xiaopeng Tang2*, Dun Deng3, and Rejun Fang1*
...sition, dry matter (DM) digestibility, metabolic energy (ME) were determined. Secondly, a total of four hundred eighty 15-day-old Shuanggui-tou meat ducks were divided into 4 treatments, 1) Control group (0% GM in the diet), 2) 3% GM group (3% GM in the diet), 3) 6% GM group (6% GM in the diet), and 4) 9% GM group (9% GM in the diet). All groups had 8 replicates and 15 birds were included in each replicate. The experiment lasted for 28 days. The average d...

Stanley Uchenna Onwudike* and Vivian Chizoba Edoziem 

...ion in Southern part of Nigeria and understanding its distributions in rhizosphere soils will help in its management. This investigation examined some variations in soil properties and inorganic phosphorus forms within the rhizosphere of three annual crops, viz groundnut (Arachis hypogea), bean (Phaseolus vulgaris) and okra (Abelmoschus esculentus) on a loamy sand, typic haplustult soil. The work was sited at the research farm of the University during 2016 pla...
Abdul Kabir¹, Amjad Hussian Mirani¹, Jam Kashif1, Shumaila Manzoor2, Abdullah Iqbal3*, Ihsan Ullah Khan4 and Muhammad Abubakar2
...e 2 years. Results of antigen detections showed that the outbreaks were going in study population and confirmed positive for PPRV by IcELISA and RT-PCR (N-gene). The study revealed a comparison of PPRV occurrence in the different production system of small ruminants and on-going disease outbreaks in the target area.
...
Muneer Abbas1*, Muhammad Ramzan1, Khalid Hussain1, Abdul Ghaffar1, Niaz Hussain1, Muhammad Irshad1, Mudassar Khaliq1 and Sohail Abbas2
... pest, Helicoverpa armigera is a major detrimental factor of gram affecting both quality and quantity of the produce in Thal regions of Punjab. Therefore, experiment was conducted to check the efficacy and feasibility of five IPM tools viz. Pheromone traps, Light traps, Trichogramma chilonis, Bacillus thuringiensis, T-shaped bird perches and Handpicking. Results showed the cumulative effects of all IPM components having 4.81, 4.96, 6.22 % ...
Amna Shoaib*, Zoia Arshad Awan and Naureen Akhtar
...i>Aspergillus minisclerotigenes and Aspergillus flavus are morphologically similar species that belong to the Aspergillus section Flavi. A. minisclerotigenes and A. flavus were isolated from soybean and okra seeds, respectively. The isolated species were first identified morphologically. ITS1–5.8S–ITS4 primers sequence and amplification of ISSR nucleotide sequences using thr...
Ahmet Ozer Sehirli1,2, Serkan Sayiner3*, Ayliz Velioglu-Ogunc4, Nedime Serakinci5, Emel Eksioglu-Demiralp6, Berrak Yegen7, Feriha Ercan8 andGoksel Sener2
...sides tissue luminol, lucigenin, malondialdehyde (MDA) and glutathione (GSH) levels. A significant difference was determined between the CRF group and the control group with regard to heart rate, blood pressure, serum creatinine, BUN, LDH, cytokines and urinary electrolyte levels. Furthermore, monocyte and neutrophil apoptosis, tissue luminol, lucigenin, malondialdehyde and collagen levels were found to increase. Tissue glut...

Shazia Mansoor1, Muhammad Sohail2*, Saima Aslam3 and Muhammad Nauman ul Islam4 

... Protein (CP) and Total Digestible Nutrients (TDN) supplementation to pregnant ewes improves the birth weight, growth rate of lambs and body condition scoring of ewes. 

...
Anila Naz Soomro1*, Hiroshi Suzuki2, Sadaf Tabassum Qureshi3 and Wazir Ali Baloch1
.... Also C. typus ovigerous females exhibiting spawning migration to the downstream and its juvenile also migrate back to upstream after completing the larval development in Urbaru stream were also observed. Presence of C. sakishsimensis at only St. 1 indicates the species is not using the river continuum for migration. Due to cave dwelling habit of C. sakishsimensis and coral origin of Kikai-jima Island it can be concluded that the species ...
Godwin Ikechukwu Ngwu1*, Maria Ifeyinwa Ngwu2, Fabian Chukwuemenam Okafor1, Aruh Ottah Anaga3 and Christopher Didigwu Nwani1
Housh Muhammad Solangi1, Javaid Ali Gadahi1*, Mansoor Tariq2, Bachal Bhutto1Zubair Ahmed Laghari1, Jamila Soomro3, Taufeeq Ahhmed Khosa1 and Abdullah G Arijo1
... by using the HcCP as antigen. Infective stage of the H. contortus (L3) was used for the challenge infection. Protein band pattern ranging from 10 to 170 kDa was observed and protein bands at 10-20 kDa, 24, 26 and 40-60 kDa were recognized by the antibodies against HcCP through western blot. Feacal egg count reduction test (FECRT) revealed that egg production was significantly reduced as 75.06%, 76.36% and 57.01 % respectively in vaccinated group as com...
Muhammad Waseem, Tariq Mahmood*, Abid Hussain, Abdul Hamid, Faraz Akrim, Shaista Andleeb and Hira Fatima
...sus thibetanus laniger) inhabits deciduous, broad-leaved forests, thorn brush forests, and coniferous forests, occurring from 1500 up to 4,000 m above sea level. The species, in many areas of its range, is in conflict with humans, however, data on its population and economic losses are scanty in the country. We investigated its occurrence, population and human conflict in Mansehra District during 2016-2017. A total of 66 indirect signs of Asiat...
Gong Ga1,2 and SuoLang Sizhu2,*
...-PCR and qRT-PCR. HEV antigens were detected in tissues by immunohistochemistry analysis. Overall, we found that that eight serum samples (4.6%, 8/173) were positive to anti-HEV IgM antibody and Seventeen serum sample (9.8%, 17/173) were positive for anti-HEV IgG antibody. The HEV RNA positive rate was of 4.6% (8/173) in feces and serum. Moreover, the prevalence of HEV RNA was 9.5% (2/21) in swine liver, 4.8% (1/21) in swine spleen, 9.5% (2/21) in sw...
Xuhui Zhang1, Zhiyuan Sun2, Jinfeng Cai1, Guibin Wang1, Zunling Zhu1, Linguo Zhao3 and Fuliang Cao1,*
...on using Aspergillus niger on growth, antioxidant capacity and meat quality of broilers. Three hundred day-old commercial Arbor Acres (AA) broiler chicks were randomly allocated into 5 dietary treatments including five variations: control group, fed with the basal diets; NF group, the addition of 3 g/kg NF products; FR1, FR2 and FR3 groups, addition of FR products 1 g/kg, 3 g/kg and 6 g/kg, respectively. There was no difference in body weight gain, feed...

Rehman Ali Keerio1, Nighat Seema Soomro1, Aijaz Ahmed Soomro1, Mohammad Aquil Siddiqui2, Muhammad Tahir Khan2, Ghulam Shah Nizamani2*, Muhammad Nawaz Kandhro1, Maryum Siddiqui4, Hajra Khan3 and Farheen Deeba Soomro

...conomy and therefore, indigenous oil production must be augmented. Sunflower seed contains 40% of good quality oil. Hence, it can play a significant role for fulfilling the Pakistan’s oil needs. This study was conducted to identify the optimum foliar zinc (Zn) supplement for sunflower variety HO-1. The experiment was conducted at field of Oil Seeds Section, Agriculture Research Institute (ARI), Tandojam, Sindh using RCBD design with three replications. T...
Wen Qin1,2, YanGan Huang1, Lei Wang1, Gonghua Lin1, Jundong Yang1,2, Pengfei Song1,2, Hongmei Gao1,2, Jingjie Zhang1,2 and Tongzuo Zhang1,3,*
...t health, immunity, and digestion. Therefore, seasonal changes in gut microbiota can affect host digestion and metabolism in ways that help the host adapt to different forage conditions. Understanding the relationship between seasonal variations in the gut microbiota and host adaptability is a good basis for the protection of the goitered gazelle. Here, we present our findings on the diversity of the gut microbiota in fecal ...

Asad Ullah1*, Umar Sadique2, Sultan Ayaz1, Muhammad Subhan Qureshi2 and Farhan Anwar Khan

...nfection in lactating indigenous cattle/buffaloes raised on different commercial dairy farms and exposing the hazards linked with milk-born tuberculosis infection in the area where unpasteurized milk is normally used in the central zone of Khyber Pakhtunkhwa (KP), Pakistan. A total of 1225 cattle and 1175 buffaloes of more than one year were studied for the prevalence of bovine tuberculosis. A total of 1608 milk samples (793 from cattle and 815 from buffaloes)...

Muhammad Jamal Nasir*, Anwar Saeed Khan, Said Alam and Riyasat Sultan 

...opulation is based on indigenous knowledge and is for the better management of forests, grasses and agriculture resources. According to field survey, availability of fodder (grasses) at the pastures, ban on cutting of grasses and grazing in the village, management of land at pastures, refuge from scorching heat of summer are the major causes of seasonal migration. There used to be a time when almost every household use to move to pasture before the onsets of s...

Cornelius Arome Omatola*, Samson Daniel Iyeh, Shedrach James Abuh, Charles Kehinde Mofolorunsho, Martin-Luther Oseni Okolo and Phoebe Queen Akoh 

Aqsa Ahmad1, Iftikhar Ahmad1* and Malik Fiaz Hussain Ferdosi

... conducted to develop indigenous soilless substrate compositions for improved growth, flower yield, stem quality and vase life of cut antirrhinum. Two experiments comprised of seven substrate compositions with variable ratios of saw dust (SD), peanut hulls (PH) and sugarcane pressmud (a by-product of sugar industry, which is rich in nutrients) or coco-coir (CC), rice hulls (RH) and sugarcane pressmud (PM) were used. Seeds were sown in plastic plug trays contai...

Ghulam Akbar1*, Muhammad Anjum Zia1, Ali Ahmad2, Neha Arooj1 and Shahneela Nusrat

...spp. Therefore, it is antigenic in nature due to microbial source which restricts its use. However, it is necessary to truncate antigenic regions present in the SK gene for the removal of streptokinase antigenicity. The basic goal of this research work is to highlight antigenic regions founded in streptokinase molecule and to give strategy to remove thes...
Ke Zhang1, Shuai Liu2, Qiu Chen1, Yu Wang1, Li Liu1, Bingjie Li1, Kai Ma1, Xiaoya Wei1, Aijun Li3 and Junyan Li2*
... to screen novel tick antigens using two-dimensional gel electrophoresis (2-DE) followed by western blot analysis (WB) with artificial antiserum. A total of 105 Haemaphysalis longicornis (H. longicornis) ticks were selected to prepare protein samples. Some of them were used for 2-DE, and the others were used to immunize mice to collect tick antiserum for WB. Then, 2-DE followed by WB and MALDI-TOF was carried out to screen and identify tick ant
Ambrin1*, Ghulam Dastagir1, Jehan Bakht2 and Muhammad Adil1
... Klebsiella pneumonia, Shigella flexneri and Staphylococcus aureus. Highest zone of inhibition against Klebsiella pneumonia and Pseudomonas aeruginosa was shown by ethyl acetate and ethanolic extracts of O. biennis. Lowest MIC value was noted for ethyl acetate and ethanolic extracts of O. biennis against Staphylococcus epidermidis, Staphylococcus aureus, Salmonella typhi and Shigella...
Imran Khan1, Muhammad Nawaz1,*, Aftab Ahmad Anjum1, Mansur-ud-Din Ahmad2, Adnan Mehmood1, Masood Rabbani1, Amina Mustafa1 and Muhammad Asad Ali1
Sajjad Ali1,*, M. Irfan Ullah2, Asif Sajjad1, Muhammad Zeeshan Majeed2, M. Aslam Farooqi1, M. Shahid Rizwan3, Qaiser Shakeel4, Sohail Akhter1,Muhammad Raheel4 and Muhammad Arshad2
...ement. Helicoverpa armigera (Noctuidae; Lepidoptera) -being highly polyphagous- is the pest of many crops and exhibits high fecundity and migrating efficiency. The present study aimed to evaluate its physicomorphic responses towards different host plants. The highest larval and pupal weights were observed when fed on gram, Cicer arietinum (L.). The maximum (97.23%) larval survivorship was recorded on gramwhile, the lowest (56.25%) on okra,...
Lei Wang1, Lu Yang2, Lei Luo1, Yaqi Gao1*, Jian Gao1 and Ping Liu1
...cing was performed and unigenes were identified after assembly and clustering. The resulting unigenes were then compared to homologous sequences using NCBI-NR, Swiss-Prot, KOG, Pfam, eggNOG, KEGG, COG, and GO for functional annotation and classification. Some differences in the regularity of distribution and the time of eclosion of the pupa were detected between the southern and the northern populations. We obtained 142.65 G...
Shaista Abbas1,Imtiaz Rabbani1, Hafsa Zaneb2, M. Shahbaz Yousaf1, Saima Ashraf2, Abid Hussain Shahzad3, M. Afzal Rashid4 and Habib Rehman1,*
...erformance and nutrient digestibility in ruminants. However, when compared with large ruminants there is scarcity of the data reporting the effects of dietary yeast in transition goats. Therefore, a trial was planned to elucidate the effects of various inclusion levels of live dried yeast (Saccharomyces cerevisiae) on dry matter intake (DMI), body condition score (BCS), body weight (BW) and serum health biomarkers in Beetal goats during the transition p...

Hasnain Alam1*, Jabar Zaman Khan Khattak1 and Taoufik Saleh Ksiksi

...hifting towards using indigenous species for sustainable landscaping. In this study, we evaluated the germination response under osmotic stresses for four native species i.e. Atriplex leucoclada, Senna italica, Tetraena mandavillei and Tephrosia apollinea. Two osmotic agents (OA) i.e. NaCl and PEG 6000 and four osmotic levels (OL) i.e. 0, -0.2, -0.4 and -0.6 MPa were tested for each species. Data was collected for different germination traits including germina...
Dil Afroza Khanom1, Amirun Nesa1, Muhammad Abu Sayed Jewel1, Muhammad Ayenuddin Haque1, Alok Kumar Paul1, Sonia Iqbal2, Usman Atique2,3*, Lubna Alam4
... by detection after wet digestion of samples. The results displayed the hierarchy of average levels of the targeted metals in G. chapra in declining order of Zn (14.37) > Cu (12.63) >Mn (1.39) > Cr (0.64) >Pb (0.38) > Cd (0.03 mg/kg) whereas, Zn (11.89) > Cu (11.18) >Mn (1.69) > Cr (0.52) >Pb (0.19) > Cd (0.01 mg/kg) in E. vacha. The t-test indicated significant differences (P<0.05) be...

Md. Zubael Islam, Rukhsana Amin Runa and Md. Mahmudul Alam* 

...animals (56.52%). The indigenous cattle were comparatively resistant to foot diseases than crossbred animals. One to two years old cattle were mostly affected in our investigation. In the rainy season, FMD, laminitis, and sole ulcer were more common compared to those in winter and summer. The prevalence of foot diseases was higher in the stall housing system (56.52%) compared to the open pack housing system (43.48%). This study highlights the prevalence and po...
Saira Saleemi1*, Zafar Iqbal1, Abdul Nasir Khalid2
... (24.02%) and A. niger (50.52%) were isolated from fish. Aspergillus niger showed high infection and it was recorded on all 200 fish samples. Fins were the most affected parts with 39.10% infection. Intestine was the least affected part with 6.59% infection. The major reason of aspergillosis in silver carp is the use of unhealthy and unhygienic feed given to silver carp reared in earthern ponds. Due to aspe...

Korkmaz Bellitürk1, Zubair Aslam2, Ali Ahmad2* and Sami ur Rehman2

...ng, Eisenia fetida (an epigeic species), were used as the test species. The samples were collected from prepared vermicompost and raw material for chemical and physical examination. For these samples, the pH, EC, organic matter, C:N ratio, phosphorus, potassium, calcium, magnesium, iron, nitrogen, sulfur and heavy metals i.e. tin, cadmium, nickel, lead, mercury and chromium parameters were evaluated. The experimental design was Complete Randomized Design (CRD)...

Hassan Boulahyaoui1,2*, Sanaa Alaoui Amine1,3, Marouane Melloul4, Farida Hilali5, Elmostafa El-Fahime1,3, Saad Mrani1,2 and Nadia Touil1,5* 

...lar characterization, antigenic disparities and the phylogenetic analysis of unusual human rotavirus VP4 genotype detected in Moroccan children fully vaccinated with Rotarix™. After RNA virus extraction, the rotavirus VP7 and VP4 genes were amplified. The DNA was purified, sequenced and genotypes were determined using the RotaC online classification tool. A phylogenetic tree was constructed using the Maximum Likelihood method applying the Tamura 3-parame...

Olotu Titilayo Mabel*, Adeosun Idowu Jesulayomi, Kaka Mary Oluwatosin and Oladipo Elijah Kolawole 

...se strain (G-01035) and Nigerian strain (NGA-INF-2016-618) have similar branch length. The susceptibility percentage for each antiviral drug ranged from 82.35% (Tenofovir) to 64.70% (Rilpivirine) while and resistance percentage ranged from 35.29% (Rilpivirine) to 23.52% (Nevirapine). In conclusions, HIV isolates from Nigeria and Sudan have the highest and closest homology. Likewise, it has shown that Tenofovir is the most ef...

Nasrullah1*, Ahmad Nawaz Khoso1, Jamila Soomro2, Ilahi Bakhash Marghazani1, Masood-ul-Haq Kakar1, Abdul Hameed Baloch1, Sarfaraz Ahmed Brohi1 and Muhammad Asif Arain1* 

...h species. Furthermore, digestibility of DM was observed similarly among two species while, digestibility of CP was recorded higher on millet fodder compare to other fodders. The digestibility of various nutrients such as CP, NDF and ADF were significantly higher (P<0.05) in sheep compared to goat. Daily weight gain, feed efficiency and cost of production were recorded similar in both s...
Muhammad Usman Asif1*, Sadam Hussain Soomro2, Raza Muhammad1, Sikandar Ali Soomro2
...vae of Helicoverpa armigera under laboratory conditions at their low, recommended and high doses by leaf dip method. The results concluded that significantly high toxicity was shown after treatment with Neomed 3 SC which caused 100 % to the insect at its recommended (200 ml) and high dose (400 ml) after 24 hours of application followed by Voliam Flexi 300 SC causing 66.66 % and 75% mortality on its recommended (80 ml) and high dose (160 ml), respectivel...

Pravin Mishra1, Md. Muket Mahmud2, Md. Ahosanul Haque Shahid2, Alamgir Hasan2, Vivek Kumar Yadav3 and Moinul Hasan1* 

...wo and half years old indigenous calf brought with myiasis wound and bacteria associated with myiasis wound was studied and further molecular detection confirms the presence of methicillin-resistant Staphylococcus aureus from the outer part of the wound but not from the inner part. The study helps and aware the veterinarians, health-workers, and general people regarding the situation of antibiotic resistance. As maggot helps in the reduction of bacteria, this ...

Kajol, A. H. Bhat, Aasha and A. K. Chaubey

Biochemical and molecular characterization of Photorhabdus akhurstii associated with Heterorhabditis indica from Meerut, India
...nella and Helicoverpa armigera. The LD50 value of strain DH3 against G. mellonella larvae at 48 hours was 8 IJs/larva for 50% larval mortality whereas in Helicoverpa armigera it needed 13 IJs/larva, to kill 50% of the larvae.

...

M. Rufai1† , A. A.Wahab2 , Q. O. Adeshina1 , J. C. Umehokoh1 and O. O.Ologunro1

Occurrence of entomopathogenic nematodes in Osun State, Southwestern Nigeria
...atodes from Osun State, Nigeria.

...

K. Osei1† , A. I. Adama1 , E. C. Tagoe2 and J. Sackey-Asante1

 

Biochar effect on nematodes and insects population density, soil improvement and yield of okra
...st of okra in Ghana and Nigeria (Obeng-Ofori & Sackey, 2003; AsareBediako et al., 2014a, Mobolade et al., 2014). It has been estimated that insect pests attack led to great reduction in okra yield (Echezona & Offordile, 2011). 

...

Sahib Khan1, Muneeza Wahid2, Muhammad Abeer Irfan1, Muhammad Naeem3, Asma Gul4, Muhammad Haseeb Zafar2

ROW LEVEL IMAGE FORGERY DETECTION TECHNIQUE USING EMBEDDED DIGITAL SIGNATURES
...gnatures. Using message digest 5 algorithm, digital signature is generated from selected pixels of each row using selected pixels for and embedded in the least significant bits of selected pixel of the corre- sponding row of digital image. The proposed technique is powerful enough to detect different image manipulations. The results show that it can successfully detect one least significant bit alteration made in any pixel of digital image.

...

Muhammad Alam Zeb Khan1*, Muhammad Ali Kamran1, Khan Muhammad2, Khizar Azam1 

IDENTIFICATION OF THE OPTIMAL OPERATING PARAMETERS OF LOCALLY AVAILABLE COAL FOR USE IN PAKISTANI INDUSTRIES
...ective utilization of indigenous coal reserves of Pakistan.

...

Zeeshan Al Hameed1, Junaid Saleem2*, Syed Sajid Hussain1, Ahsan Abdul Ghani2, Hira Lal2 

STUDY OF INDIGENOUS FLUORSPAR AS METALLURGICAL FLUX
...in Pakistan, that the indigenous Fluorspar can directly be used as a metallurgical
flux without any beneficiation process as it is rich in Fluorite and also contains small amount of other compounds
that are required to be fed as part of slag. Specifically, it contains only trace amount of apatite as compared to
global Fluorspar. The composition of indigenous Fluorspar was compared with standard specifica...

 Waqas Javaid1*, Adnan Tariq1, Iftikhar Hussain2, Sahar Noor2

AN EVOLUTIONARY APPROACH FOR CMS DESIGN
...sist of Artificial Intelligence
(AI) based techniques. The main advantage of such approaches is their ability to handle the CFP effectively both in
terms of accuracy and computational effort. Following the same trend an evolutionary algorithm has been developed
during this research by combining Standard Genetic Algorithm with a very effective Local Search Heuristic (LSH).
The results show that it is efficient both in terms accuracy ...

Parsa Riaz and Muhammad Naeem*

Digestive Enzymes Activity with Gut Morphometric Parameter of Carnivorous Fish Wallago attu (Siluridae, Siluriformes)
...n and levels of various digestive enzymes, such as lipases, proteases and amylases in the digestive tract of a wild, freshwater, carnivorous Wallago attu. Wallago attu is a fast growing catfish that belongs to family siluridae under the order siluriformes. It has good market demand as a food fish having high nutritional value, and high protein content in its flesh, Carnivorous in nature. Descriptive data of the studied trait...

Muhammad Usman Shafi*, Syed Muhammad Ali**, Syed Akhtar Ali Shah***, Kamran Ahmed****

DEVELOPMENT OF DATABASE OF HEAVY TRUCK LOAD DATA IN PESHAWAR, PAKISTAN
...ject is to develop an indigenous solution that should be efficient and cost effective

for determining truck loads passing from Peshawar. As heavy vehicles impacts road most, therefore small vehicles
are put in one category and trucks are divided into two, three, four, five and six axles to give clear interpretation
of each type of truck. To determine load data, a portable weighing station was designed keeping in view the size ...

 Sikandar Afridi*, Muhammad Irfan Khattak**, Nasim Ullah***, Gulzar Ahmad**, Muhammad Shafi**

PERFORMANCE ENHANCEMENT OF FACE RECOGNITION SYSTEM USING PRINCIPAL COMPONENT ANALYSIS MERGED WITH DISCRETE WAVELET TRANSFORMS
...cted by calculating the Eigenface of the image with size already
reduced by taking DWT. As a result of this process the size of database will reduce to one-fourth of the conventional
PCA in which the facial features are extracted directly from the image by calculating the Eigenface. The size of the
train database is reduced with the proposed technique which reduces the processing time of t...

Nisar Mohammad٭, Mohammad Mansoor Khan٭, Noor Mohammad٭

EXTRACTION OF HIGH QUALITY TALC FROM TALC-CARBONATE ROCK OF MINGORA EMERALD MINES BY FLOTATION AND LEACHING
...e for up gradation of indigenous noncommercial talc resources from other locations. These rocks host emerald crystals and the debris are dumped after excavation and extraction of emerald crystals. Controll of environmental threat caused by these rock dumps is an additional benefit of the research. Two step froth flotation process with AF65 (Poly propylene Glycol, Cyanamid) as frother, Sodium hexa metaphosphate as depressant for carbonates, equal mixture o...

Feroza Arshad*, Aftab Ahmed Memon**, Muhammad Aslam Uqaili** and Arshad Habib Malik*

SYNTHESIS OF A NOVEL ROBUST INTELLIGENT CASCADED REACTIVITY CONTROLLER FOR CANDU REACTOR
...his paper, a Novel Intelligent Cascaded Reactivity Controller (NICRC) is synthesized for a CANadian Deuterium Uranium (CANDU) -type Nuclear Power Plant (NPP) operating in Pakistan. The designed NICRC is a cascaded configuration of reactor power and moderator level controllers composed of five sub-controllers. The proposed NICRC is designed using intelligent soft computing technique. The original reactivity con...
Muhammad Shaheen
APPLICATION OF LABELLED K MEANS CLUSTERING FOR GIS CONTRACT AUTOMATION
...anism for penalizing negligence of either party. This paper proposes (1). a method to regulate the technical part of the GIS contract by suggesting a simple and wizard-based Graphical user interface. (2). Conversion of existing manually prepared contracts into electronic contracts through lexeme-based congregation which is done through labelled K Means Clustering. These converted clusters are then stored into centralized database. Back Propagation Neural ...

 Sikandar Bilal Khattak, Iftikhar Hussain, Rashid Nawaz

EVALUATING THE SOCIAL ASPECT OF SUSTAINABLE CONSTRUCTION FOR PAKISTAN VIA ANALYTICAL HIERARCHY PROCESS
...the basis of their Eigen values obtained from AHP analysis. The result shows that clean water is considered in almost all of the projects whereas the universities and other research institutes are hardly considered in any projects.

...

 Riaz Sayed1, Khizar Azam1, Waqar Shah1, Inayat Babar1, Hamidullah1, and Sahar Noor1

A REVIEW OF DRIVING UNDER THE INFLUENCE OF FATIGUE
...ed. The Center for Intelligent Systems Research (CISR) of the George Washington University conducted an experiment at their Vehicle Simulator Laboratory to study driver drowsiness. Results of this study and description of the algorithm are provided. A comprehensive list of references is provided at the end.
...

Parakriti Gupta1, Kapil Goyal2 and Mini Pritam Singh3*

Hepatitis E Virus and Zoonosis
...d by detection of HEV antigen or anti-HEV IgM in acute sera. However, the detection of HEV RNA is important to establish the diagnosis in immunosuppressed patients. In transplant patients, decrease in immunosuppression is usually done, however ribavirin therapy has also shown a definitive role. There is upcoming literature on the role of sofosbuvir also in these groups of patients. Since the virus has only one serotype, univalent vaccine can provide protection...

Markus I. Francis1*, Paul I. Ankeli2, Clara N. Kwanashie1, Jibril Adamu1, Lushaikyaa Allam1, Mashood A. Raji3, Godwin O. Egwu4, Flavio Sacchini5 and Massimo Scacchia5

Detection of Mycoplasma bovis from Cattle Presented for Slaughter in Adamawa and Taraba States, Northeastern Nigeria
... cattle in northeastern Nigeria. Four hundred and eighty (480) samples of lung tissues (180), nasal swab (180), ear swab (60) and pleural fluid (60) were collected from 190 heads of cattle at slaughter in Yola and Jalingo abattoirs in Adamawa and Taraba States, respectively Samples were processed based on standard laboratory protocols. An overall Mycoplasma bovis isolation rate of 0.83% (4/480) was obtained. Based on the states studied, 1 (0.35%) and 3 (1.53%)...

Oluwatoyin Olagunju1* and Luqman Abiodun Akinbile2

Farmers Perceptions of the Effect of Rural Transportation Systems on Farming Income in Ondo State, Nigeria
...lation. Nonetheless, in Nigeria, the state of rural roads is pitiable, as the development, enhancement and provision of more rural transport systems could be a panacea for the prolonged deplorable and worsening state of rural roads in the country. This study examined the perceived effects of rural transportation systems on farmers’ income in Ondo State, Nigeria. A structured interview was used in eliciting information ...
Fei Wang1, Xianlai Zhang1, Youbao Zhong2, Xiaoen Tang3, Xiaofen Hu1
ShanShan Yang1, Shengwei Zhong1, Zuohong Zhou1, Jin Liu1, Xu Yuan1 
and Yong Li1*
...tural characteristic of pigeon intestine, the microstructure of each part of intestine was analyzed by HE staining, and its vasoactive intestinal peptide (VIP) was detected by immunohistochemistry staining and RT-qPCR. In present results, the intestinal mucosa epithelium is a single layer of columnar epithelium, other structures are relatively complete, and the intestinal villi and glands are distinct in each part of intestine. The localization of VIP is exten...
Safia Arbab1, Rehana Buriro1, Shams Uddin Bhugio1, Atta Hussain Shah2,  Jamila Soomro3,Qudratullah Kalwar4, Saqib Ali Fazilani1 and Waseem Ali Vistro5*
...S. aureus, E. coli, Shigella and Salmonella spp respectively. However, out of 50 sample, 34 (68%) were found to have different bacterial species and 16 (32%) were recorded with pure contamination. At 30 µl of crude Aloe Vera isolated bacterial organisms did not show any susceptibility, whereas at 60 µl, organisms showed quit sensitive reactions. In contrast, ethanol extract of Aloe Vera showed better result as compared to crude Aloe V...
Zengwen Huang1,2, WuReliHazi Hazihan2*, Baheti Bodai2, Kadyken Rizabek3, Nuralieva Ulzhan3, Omarova Karlygash4, Juan Zhang1 and Yaling Gu1*
...A expression vector was digested, identified and sequenced, and then transfected into YM sheep granulosa cells. The sequence of YM sheep INHα gene was obtained and uploaded on the NCBI database (accession number KP-113678.1). The INHα gene from YM sheep is 1109 bp long and translates to approximately 40 KDa protein. Q-PCR analysis showed that the plasmid pGenesil 10-3p-siRNA could interfere with the expression of INHα in granulosa cells to an...
Aamir Khan1, Rabia Noushin2, Mohammad Attaullah3, Shahid Naiz Khan1, Rafiqe Hussain4, Farman Ullah Dawar1, Faiz Ur Rehman1, Muhammad Ijaz1 and Kalim Ullah1*
...nce (35.46%) than B. bigemina (19.33%). Females were more prone as compared to male cattle for both B. bovesandB. bigemina. Cattle having age less than two years showed high positivity for bothB. boves (49.23%)and B. bigemina (26.15%). Similarly rate of positivity for both B. boves (47.12%)andB. bigemin...

Olugbenga Omotayo Alabi1*, Ayoola Olugbenga Oladele2 and Ibrahim Maharazu3

Profitability Analysis and Marketing Efficiency of Soyabean (Glycine max) Value Chain among Actors in Abuja, Nigeria
... among actors in Abuja, Nigeria. This study was designed specifically to achieve the following objectives: describe the socio-economic characteristics of soyabean marketers, determine the marketing cost, marketing margin, and marketing efficiency of soyabean, analyze the costs and returns of marketing soyabean, evaluate factors influencing or affecting marketing efficiency of soyabean, and identify problems or constraints influencing marketing of soyabean. Dat...
Sehrish Firyal1,*, Ali Raza Awan1, Muhammad Umair Latif1, Muhammad Tayyab1, Muhammad Wasim1, Shagufta Saeed1 and Imaad Rashid2

 

...ni domestic breed Lathy pigeon. Cytb haplotype for local domestic breed was developed. Homology analysis of Cytb gene revealed Columbia livia as closest homologue of Pakistani Lathy pigeon with five novel SNPs. Phylogenetic analysis of domestic lathy pigeon indicated the monophyletic relation with C. livia. We are reporting for the first time, the novel SNPs in ...

Pankaj Kumar Jha1,2*, M. Golam Shahi Alam1 and Farida Yeasmin Bari1

Pregnancy and Lambing Rate Following Laparoscopic Artificial Insemination with Two Different Types of Diluent and Frozen-thawed Sperm Dose in Ewes
...emination (LAP-AI) in indigenous ewes in Bangladesh were studied. A total of 78 non- pregnant ewes were synchronized for oestrus with two doses of 100 μg PGF2α (Cloprostenol) 9 days apart. The ewes were randomly assigned to one of four groups; G1 (n = 18; commercial Triladyl®/ 100 × 106 sperm), G2 (n = 21; commercial Triladyl®/ 50 × 106 sperm), G3 (n = 20; home-made tris based/ 100 × 106 sperm) and G4 (n = 19; home-made tris ...

Paul Oludare Adetunji* and Safiriyu Idowu Ola

Comparative Productive Performance among three Plumage Varieties of Noiler Hens
...Noiler is an improved indigenous dual-purpose breed of chicken developed in Nigeria to meet national demands for food supply. The objective of this study is to compare the productive performance among three prominent plumage varieties of Noiler hens. For this purpose, a total of 90 pullets with three plumage colours (brown, black and barred) were divided into 3 replicates with 10 birds per replicate in each of the variety. D...

Rao Wali Muhammad1, Hafiz Muhammad Wasif Ali2*, Amir Hamza1, Muhammad Qadir Ahmad2, Abdul Qayyum2, Waqas Malik2 and Etrat Noor2

Estimation of Different Genetic Parameters in Various Safflower (Carthamus tinctorius L.) Genotypes under Field Condition
...een studied parameters. Eigen values >1 contributed variability between the genotypes under field condition. Minimum and maximum heritability estimates ranged between 77.4% - 99.0% for days to flowering and pods per plant, respectively. Genetic advance estimates ranged between 11.99-27.21% for number of primary branches per plant and yield per plant, respectively. However, coefficient of variance ranged between 2.67-19.31% for days to maturity and number of...

Joseph Anejo-Okopi1*, Obinna Oragwa Arthur2, Ocheme Julius Okojokwu1, Sarah Joseph1, Geoffrey Chibueze1, Joshua Adetunji1, Joseph Ameh Okwori3, David Ochola Amanyi4, Otobo I. Ujah5 and Onyemocho Audu6

Seroprevalence of Rift Valley Fever Virus Infection among Slaughtered Ruminants in Jos, North-Central, Nigeria
... in Jos, North-Central, Nigeria. Blood samples were collected from 100 livestock (cattle and goats) at selected slaughter locations in Jos Metropolis. Questionnaires were administered to obtain information on animal species, sex, and localities of origin. The blood samples were screened for RVFV antibodies using competitive Enzyme Linked-immunosorbent Assays (C-ELISA) to detect anti-RVFV IgG/IgM. Eleven out of 100 samples tested positive for anti-RVFV antibodi...

Khan Sher1*, Muhammad Subhan1, Muhammad Nisar2, Ali Hazrat2, Zahid Fazal1, Gul Rahim2, Imran Ahmad1, Riaz ul Haq1 and Shamia Bibi1

Genetic Diversity in Common Beans (Phaseolus vulgarus L.) Collected from Different Ecological Zones of Malakand Division (A Part of the Sino Japanese Region of Pakistan)
...mon bean landraces. The eigenvalues of more than 60.8% accounted a total of 70% genetic variants. Finally, it was found that a high degree of variance was identified in all germplasm collected from Malakand sections with some additional unique characteristics, such as early cooking, high productivity and protein significance as compared to other parts of the world, which could be utilized for evolving better quality and high yielding cultivars of P. vulgarus.<...

Joseph Anejo-Okopi1*, Dorcas Yilger Gotom1, Noble Allison Chiehiura1, Julius Ocheme Okojokwu1, David Ochola Amanyi2, John Otumala Egbere1, Joshua Adetunji1, Otobo Innocent Ujah3 and Onyemocho Audu4

The Seroprevalence of Zika Virus Infection among HIV Positive and HIV Negative Pregnant Women in Jos, Nigeria
...s in Jos, North Central Nigeria. A cross-sectional study was performed at Plateau State Specialist Hospital in Jos, North Central Nigeria from August to December 2020. We enrolled a total of 90 (45 HIV positive and 45 HIV negative) pregnant women aged ≥18 and ≤50 years attending antenatal clinic. Ethical approval was obtained and approved by the Health Research Ethics Committee of the hospital. Detection of ZIKV IgG an...

Saeed Ahmad1, Abdul Ghaffar1, Muhammad Habib ur Rahman1,3*, Tanveer-ul-Haq2, Mahmood Alam Khan4 and Arshad Mahmood5

Evaluation of Different Production Systems in Combination with Foliar Sulphur Application for Sunflower (Helianthus annuus L.) under Arid Climatic Conditions of Pakistan
...o lower production of indigenous edible oil, a huge quantity is imported which has a negative effect on the economy. To reduce the import expenses and meet country’s demand, there is a need to increase area under oilseed crops without disturbing the cropping systems. Climate variability is another emerging threat for current production and cropping systems especially in south regions of Pakistan having high arid climatic conditions. A study was conducted...

Mehnaz Safdar and Urooba Pervaiz*

Constraints in Accessing Agricultural Extension Services by Rural Women: Evidence from Khyber Pakhtunkhwa, Pakistan
...y alarming and shows negligence of extension department. These trainings were provided by various NGOs and private organizations. Those women who got trainings were more updated than those who did not attend or had no access to trainings. Chi-square analysis revealed that age had significant association with involvement in decision making. It means that by increasing age of the respondents, the tendency towards involvement in decision-making increases. Literac...
Shakeel Ahmed* and Sarwat Jahan
...omedin S (NMS), an anorexigenic neuropeptide was first discovered in rat brain. It is a ligand for receptor FM4/TGR-1 which is also called as NMU receptor type II (NMU2R). Mainly it is expressed in SCN and involved in regulation of food intake and dark light circadian rhythms. In rodents and higher primates its stimulatory role in HPG axis is reported. Growth hormone (GH) is released from anterior pituitary and directly or indirectly play very important role i...
Taqiur Rahman1, Tabassum Yaseen1*, Ali Mujtaba Shah2, Samiullah3, Ghulam Jelani3, Gul Nawaz1,Qudratullah Kalwar2
...mentation of accurate indigenous knowledge. (R1andR2) All the plant species have high medicinal values and used as an ethno veterinary by the local inhabitants. Like However, family Lamiaceae showed remarkable medicinal benefits. The administrative route is virtually oral, while some fifty species are used externally for skin infections. This study concluded that Shangla District is a rich source of herbal awareness and medicinal plants. In local interviews, i...

Tasleem Akhtar1, 2, Ghazanfar Ali1,* and Nuzhat Shafi2

... one new species (S. niger) was found first time in AJK. One hundred and fifty-nine specimens of S. plagiostomus, 74 specimens of S. esocinus, 21 specimens of S. progastus and 8 specimens of S. niger were taken for the study. The maximum snout length, eye diameter, length of lateral line, head length, dead depth and pre anal length were observed in S. niger
Muhammad Zeeshan Majeed1*, Muhammad Shahzad Akbar1, Muhammad Afzal1, Muhammad Mustaqeem2, Muhammad Luqman3, Ijaz Asghar4 and Muhammada Asam Riaz1
...essential oils of ten indigenous plant species were evaluated for their insecticidal and repellency potential against the subterranean termite Odontotermes obesus Ramb, a destructive insect pest of wooden infrastructures, agricultural crops, orchards and forest plantations. Standard filter paper disc method was used for both toxicity and repellency bioassays according to completely randomized design. The response of termite workers varied with plant spe...
Muhammad Zeeshan Majeed1*, Muhammad Afzal1,Muhammad Asam Riaz1,Kanwer Shahzad Ahmed1, Muhammad Luqman2, Mehar Zubair Shehzad1, Muhammad Bilal Tayyab1, Mujahid Tanvir1 and Saadia Wahid1
...cticidal potential of indigenous ethnomedicinal flora of Soone Valley and surrounding Salt Range of Pakistan. Acetone extracts (10%) of forty plant species were evaluated against Asian citrus psyllid (Diaphorina citri), armyworm (Spodoptera litura), house mosquito (Culex quinquefasciatus) and subterranean termite (Odontotermes obesus) using twig-dip, leaf-dip, aqueous exposure and filter paper-dip bioassay methods, respectively. Res...

Agustine Christela Melviana1, Rizkita Rachmi Esyanti1*, Roy Hendroko Setyobudi2, Maizirwan Mel3, Praptiningsih Gamawati Adinurani4 and Juris Burlakovs5

Gene Expression Related to Steviol Glycoside Synthesis Produced in Stevia rebaudiana (Bert.) Shoot Culture Induced with High Far-Red LED Light in TIS RITA® Bioreactor System
...ards biomass growth, multigene expression related to steviol glycosides, and its derivatives, and also the metabolites produced in S. rebaudiana plant. The result showed that the increment of biomass and gene expressions (ent-KO, ent-KS, ent-KAH13, UGT85C2, UGT74G1, and UGT76G1) in high far-red LED RITA® was higher compared with control RITA®. To conclude, the system with a far-red LED induction in TIS RITA® was proven to give a positive effect tow...

Mahmoud Mohamed Ahmed Youssef* and Suzan Abd-Elazeim Hassabo

The Role of Genetic Engineering in Management of Plant Parasitic Nematodes with Emphasis on Root-Knot Nematodes: A Review
...tode physiology such as digestive enzymes or structural proteins of the intestine are considered.

...
Syed Zakir Hussain Shah1*, Muhammad Afzal2, Mahroze Fatima3Syed Makhdoom Hussain4 and Tanveer Ahmed5
...de fat and gross energy digestibility by fingerlings when fed PHY sprayed diet. Similarly, CA addition in the diet resulted in increased (p<0.05) digestibility of these nutrients. Also, the minerals digestibility was significantly (p<0.05) affected by top spraying of phytase on the diet pellets. Fish having CA acidified diet also showed improved (p<0.05...

Amjed Ali1, Rafi Qamar1*, Muhammad Ehsan Safdar1, Salman Saleem2, Sami Ullah3, Muhammad Arshad Javed4 and Syed Wasim Hasan5

Development and Growth: Influence of Sowing Dates on Performance of Cotton Cultivars
...gment its production. Indigenous cultivars have an impressive production potential while climatic sowing dates fluctuation during crop planting is the leading hindrance in its production. Therefore, this one-year field experiment was performed to assess the result of varying cultivars and sowing dates on developmental and allometric traits of cotton (Gossypium hirsutum L.) under the agro-ecological conditions at Sargodha, Punjab, during Kharif season, 2015. Th...
Muhammad Saeed Shah1, Fazal Nasir2, Sana Ul Haq1 and Muhammad Tahir Khan2*
... body against harmful antigens. In this paper, the concept of biological immune system, idiotypic network theory and clonal selection theory are applied in multi-robot cooperation. Idiotypic network theory governs the stimulation and suppression of an antibody in response to an antigen. Clonal selection theory represents how immune cells mutate and proliferate into memory cells. The methodology presented in this paper is aut...

Muhammad Shaheen

Mining Patterns from Complex Energy Dataset: A Case Study of Pakistan’s Energy Sector
...posed an automated intelligent geographic decision support system that ensures optimal energy development plan for the country in order to cope with the current energy crisis. The paper proposed data mining add-ins for its inclusion on GIS interface.

...
Saima Yaqub1, Tahir Yaqub1*, Muhammad Zubair Shabbir2, Asif Nadeem3Aziz-Ul-Rahman1, Muhammad Furqan Shahid1, Zarfishan Tahir4 and Nadia Mukhtar4
...b Combo test and then antigen positive samples were amplified for HIV-1 pol gene (1084 bp) and analyzed. Initial screening showed a total of 49 HIV samples positive (24.37%; 95% CI: 18.96-30.76). A substantial association of HIV incidence was observed in individuals with HCV infection (36.84%; 95% CI: 28.55-45.99; p<0.0001) followed by individuals involved in practices of shared injection equipment (21.17%; 95% CI: 25.07-40.21; p<0.0001), i...

Yuan Xing, Congming Tan, Yan Luo and Wenjun Liu* 

Chibuzo Uzoma Izuogu1*, Robert Ugochukwu Onyeneke1, Loveday Chukwudi Njoku1, Gillian Chidozie Azuamairo1 and Mary Chikerenma Atasie2

 

Repositioning Nigeria’s Agricultural Extension System Towards Building Climate Change Resilience
...l extension practice in Nigeria in response to the challenges of climate change. We reviewed 58 articles from research databases that were in line with our inclusion criteria for adequate understanding of the current state of agricultural extension practice in Nigeria and climate change concerns. The review identified the effects of climate change on agricultural extension practice, ascertained the training needs of agricult...

Ambreen Akhtar Saddozai1, Amer Mumtaz2, Naseem Rauf1, Saeeda Raza2, Nouman Rashid Siddiqui2, Muhammad Naeem Safdar2, Sahar Shibli2, Muhammad Suhail Ibrahim3*, Muhammad Akhtar4 and Muhammad Saad Rehan5

Preparation and Quality Evaluation of Soymilk Carrot Blend
...ratures (ambient and refrigerated temperature) for 14 days to assess its shelf life. During the storage period the samples were analyzed for different chemical parameters total soluble solids, pH, total carotenoids, protein contents and antimicrobial analysis. On the basis of quality attributes it was observed that plain and carrot supplemented soymilks remained acceptable up to 7 days at refrigerated temperature. Plain soym...

Muhammad Tariq Mahmood1*, Muhammad Akhtar2, Mushtaq Ahmad1, Muhammad Saleem2, Ali Aziz2, Irfan Rasool2, Zeshan Ali3 and Muhammad Amin2

An Update on Biology, Extent of Damage and Effective Management Strategies of Chickpea Pod Borer (Helicoverpa armigera)
...justify;">Helicoverpa armigera is considered as widespread and cosmopolitan insect responsible for drastic decline in chickpea productivity across the world. Management of H. armigera is of prime importance to achieve sustainable chickpea yields. Its life cycle passes through egg, larvae, pupae and adult stages in about 4-5 weeks. 1st to 3rd instar larvae generally feed on leaves, twigs and flowers. In later stages larger la...

Muhammad Rizwan1*, Jehanzeb Farooq1, Muhammad Farooq1,2, Aqeel Sarwar4, Abid Ali3, Farrukh Ilahi1, Muhammad Asif1 and Ghulam Sarwar1

Quantitative Studies in Upland Cotton (Gossypium hirsutum L.) using Multivariate Techniques
...t analysis (PCA) having eigenvalue more than 1, showed 91.131 % the cumulative variance, while PC-1 alone showed 32.47 % variance. Hierarchical cluster analysis and PCA provided an opportunity to identify subgroups of clusters at different stages, so that every single subgroup may be analyzed critically and it will be helpful for incorporation of desirable characters in future breeding programmes.

...
Wentao Wang1,2, Xu Lin3, Jianshu Zhuo3, Dongjie Zhang2, Xiuqin Yang3* and Di Liu1, 2*
...ne or in combination. Minigene analysis showed that the splicing of porcine E2F3b is complicated. E2F3b isoforms are expressed in all tissues studied with high level in spleen and muscle. Both of isoforms V1 and 2, containing functional domains of E2Fs, were localized throughout cells. No functional nuclear localization sequence and export signal were characterized through site-directed mutagenesis analysis, although their existence was predicted by bioinforma...
Ayesha Alam1,* and Labeeb Ali2
...sorders, bronchiolitis, digestive tract disorders, pneumonia and conjunctivitis. Additionally, traces of COVID-19 were also found in sewage water sources in some countries like Italy bringing attention towards analysis of sewage water. Based on information from cited literature it is estimated that an individual handling sewage water have approximately 1% chance of becoming infected with virus. Treated sewage effluent and reuse of sewage water for recycle purp...

Muhammad Shahbaz1,*, Nosheen Farooq1, Abu ul Hassan Faiz1, Arshad Javid2, Irfan Baboo3, Misbah Shoukat1 and Muhammad Aslam Khan4 

..., Ae. micropterus and Armigeres subalbatus (subfamily culicinae). The most abundant species was Armigeres subalbatus.
...

Saba Aleem1*, Mehvish Tahir2, Iram Sharif3, Muqadas Aleem4, Muhammad Najeebullah2, Ali Nawaz1, Amina Batool1, Muhammad Imran Khan1 and Waheed Arshad1

Principal Component and Cluster Analyses as Tools in the Assessment of Genetic Diversity for Late Season Cauliflower Genotypes
...rincipal components had eigenvalue >1 and contributed 80.86% of the variation among the genotypes. Traits i.e. plant weight, curd weight, curd yield, leaf length, plant height to extreme, and the number of leaves contributed significant positive component loading to these principal components (PCs). Biplot analysis among the first two PCs found the Casper RZ, AA Cauli-08, and Snow queen as diverse genotypes. Cluster analysis based on Euclidian distance grou...

Muhammad Umair Hassan1, Muhammad Aamer1, Muhammad Nawaz2, Abdul Rehman3, Talha Aslam3, Ubaid Afzal4, Bilal Ahmad Shahzad3, Muhammad Ahsin Ayub5, Faryal Ahmed1, Ma Qiaoying1, Su Qitao1 and Huang Guoqin1*

Agronomic Bio-Fortification of Wheat to Combat Zinc Deficiency in Developing Countries
...s blindness, lower intelligence quotient (IQ) levels, weaker immune system, and impaired physical and mental development. Wheat crop play a chief role in daily food requirement and calories need in developing countries, however, inherently wheat has lower Zn contents. Moreover, soil Zn deficiency further increasing the problem of low Zn contents in wheat grain. Thus, the finest way to combat the Zn deficiency is to produce the grains of wheat having desirable ...
Ghulam Sarwar1, Amna Nazir1*, Muhammad Rizwan1, Eram Shahzadi2 and Abid Mahmood3
...ents were selected with Eigen value ˃1. The four principal components contributed 79.9% towards variability. In PC-I, there was maximum positive factor loading of earliness traits, fiber fineness and negative factor loading of sympodia per plant and seed cotton yield. In PC-II, there was maximum positive factor l...

Mansoor Qadir1*, Yousaf Khan2, Shahid Khan3, Djamaleddine Djeldjli3 and Muhanned Ismael Ibrahim Al Firas4

...d by deploying diagonal eigenvalue unity (DEU) scheme at the frequency domain (X-axis) in combination with double weight zero cross-correlation (DW-ZCC) code at the time domain (Y-axis). The proposed 2D-DZ coding scheme provides high orthogonality in adjacent codes and resolves the problem of larger code lengths. The new coding scheme and corresponding network architecture is analyzed in terms of Gaussian Approximation analysis and simulation through OptiSyste...
Abdul Hafeez1*, Akhtar Nawaz Khan2 and Zahid Ullah3
...well as to instill intelligence in the approach to reduce subjectivity to a greater extent. To harness the high computational power offered by commodity graphics processing units (GPU) and accomplish the decision-making in real-time has gained traction in a gamut of recent biomedical applications. This review article investigates the use of state-of-the-art GPUs to benefit the biomedical community through real-time decision-making from huge amount of biomedica...
Huan Yang1, Tingting Li1, Huimin Zhao1,2, Xiaoyuan Zhang1, Huiyan Xu1, Yangqing Lu1, Xingwei Liang1, Shengsheng Lu1, Xiaogan Yang1*and Kehuan Lu1*
...month-old buffalos were digested with two-step enzymatic treatment to obtain isolated single cells and then enriched with a differential plating method. Isolated SSCs were cultured on STO feeder cells with SSCs culture medium (SSCM), which is serum-free. SSCs began to proliferate on the second day and quickly formed grape-like clusters that were consistent with the morphological features of SSCs. These cells were identified as positive by immunofluorescence st...
Faheem Ahmed Khan1*, Sarzamin Khan2, Qurat-ul-Ain3, Saqib Ishaq1Muhammad Salman1, Abdul Rehman1, Ikram Ullah4, Kalsoom5 and Johar Jamil5
...le cheap sources. The indigenous Saccharomyces cerevisiae were isolated from different fruit samples, identified by conventional polymerase chain reaction (PCR) and were characterized for production of single cell microbial protein (SCMP). Out of 60 different fruit samples, a total number of confirmed S. cerevisiae isolates were 1, 2 and 1 from orange (Citrus sinensis), grapes (Vitis), and peach (Prunus persica), respectively...
Ye Ge1, Chunmiao Zhao1, Weitian Xie2, Ying Liu2,* and Chunhou Xu1,*
...ly producing spores and digestive enzymes, we produced the protoplast as the parent strain of B. subtilis and Rhamnose lactobacillus and analyzed the production rate of spores, the ability to resist high temperature and hereditary stability. The results showed that RH-3 was a perfect protoplast from which 48 strains were obtained through regeneration, with a sporogenic rate of 69.1% and a colony number of 2.7×105 CFU/mL after an ...
Guofang Wu1,2, Xingxing Xue1, Wenjuan Shen1, Lei Wang1,2*, Yuhong Ma1 and Jiping Zhou1
...Bamei pig (n=256), an indigenous breed of Chinese pig. TOF-MS demonstrated that the L1-(GT)n locus had three alleles: 152 bp, 164 bp, and 166 bp. The three other loci, L2-(AC)n, L3-(TC)n, and L4-(TGA)n, all had two alleles. Alignment of our sequencing data with the Sus scrofa reference genome (Sscrofa11.1) revealed that the L1-(GT)n microsatellite, which features a GT repeat motif, was located in th...
Eman A. Al-Shahari1,2, Abdelhalim A. Alkhazendar3, Eman R. ElBealy4 and Abeer A. Alm-Eldeen3*
...ferating cell nuclear antigen (PCNA). After quercetin administration, they showed an improvement in the previous biochemical parameters. Seminiferous tubule diameters were 105.2 ± 2.1 µm and their epithelium heights were 26 ± 2.1 µm. Remarkably, spermatogonia, spermatocytes, spermatids and sertoli were positively reacted with PCNA. In conclusion, quercetin could modulate aging induced-testicular damage via increasing the antioxidant d...

Natalija Grittner1, Radomir Mandić2,* and Milivoje Urošević3

... of populations these indigenous breeds in Serbia has been shown in this paper. It is proposed that 15 breeds (13 breeds and 2 strains), which are not protected by the Decree of the Ministry of Agriculture of Serbia on animal genetic resources, be included in the Decree in the future, in order to preserve and improve their numbers. An urgent establishment of the animal gene bank of Serbia has been also proposed. It is given a proposal of measures for further a...

Faiza Aman1,*, Neelam Ara1 and Syed Mehar Ali Shah2

 

 

...rincipal components had Eigen values > 1 accounting for 78.9 % of total variation. The first principal component was characterized by pods plant-1, green pod yield, plant height and hundred green seeds weight. The second principal component was dominated by pod length, hundred green pods weight, seeds pod-1, hundred green seeds weight and days to first picking. Cluster analysis for quantitative traits grouped pea genotypes into five di...
Sheza Shehzadi1, Mohammad Umar Farooq2*, Rukhsana Kausar1, Ijaz Ali1*, Muhammad Arshad Ullah3 and Maqbool Shahbaz4
...ther extract, and total digestible nutrients. Significant results were obtained by applying ANOVA test on the data retrieved. All three factors (BP, CC and CS) showed maximum results after 60 days of growth. The result indicated that after the 60 days interval the animal unit per month gave the maximum fodder (7.0667 AU/M/Ha) that proposed to be grazed for large ruminants and then 21 small ruminants on the same biomass and also maximum forage production record...
Grace Okongor, Chukwudi Njoku*, Pauline Essoka and Joel Efiong
...a of Cross River State, Nigeria. Rainfall, temperature, sunshine and relative humidity (1976-2016), as well as yam yield data (1990-2016) were considered. Results revealed that climate elements fluctuated steadily during the period with a marginal increase in yam yield. The projections from the Time Series Modeler showed that relative humidity will decrease slightly while yam yield will increase by an additional 265 metric tons in 2026. Although minimal, and n...
Roland Eric Yessinou1*, Alain Richi Kamga Waladjo2, Nestor Noudeke1, Ignace Dramou3, Justin Adinsi1, Victorien Tamègnon Dougnon4, Elsie Yélognisê Sangnidjo5, Razack Osse5, Alfred Dansou6 and Souaïbou Farougou1
...in several countries as Nigeria (12-42%), followed by Sierra Leone (8-43%), and Guinea (9-40%). Pooled prevalence of LASV in human populations in studies conducted over the entire review period was 22.0% [95% CI (17.0-28.0 %), I2 = 98.0%]; eighteen studies), while she was 9.0% [95% CI (4.0-15.0 %), I2 = 97.0%]; eight studies) for Mastomysspp. The knowledge of the geographical distribution and epidemiology may help disease con...
Asma Chaudhary1, Qurat-ul-Ain Ahmad1, Afia Muhammad Akram1, Sadia Roshan2 and Javed Iqbal Qazi3*
...rying and storing in refrigerator. Mortalitiesof Labeo rohita fingerlings challenged to P. fluorescens intraperitoneally appeared in a dose dependent manner with symptoms like hemorrhage, swelling of the belly and anus, scale damage etc. The pathogen challenged fish were administered with Sphingomonas sp.AsCh-P3 (group-G1) and B. aerius AsCh-A7 (group-G2) treated feed in different experimental set ups. Highest Relative Percent Survi...

Joseph Anejo-Okopi1*, Jennifer Ifeoma Okpara1, James Mogoret Dabol1, Joshua Adetunji1, David Adeniyi2, David O. Amanyi3, Otobo I. Ujah4, Veronica David1, Patience Omaiye5 and Onyemocho Audu6

...fected patients in Jos, Nigeria. This cross-sectional study was conducted on ninety (45 HBV/HBV and 45 HBV-mono) infected patients with chronic hepatitis B attending the hepatitis clinics of the two tertiary hospitals (Plateau State Teaching Hospital and HIV treatment center, at Jos University Teaching Hospital), Jos Metropolis from September to December 2018. The anti-HDV antibodies (IgM) were assayed using enzyme-linked immunosorbent assay HDV IgM ELISA Kit ...

Saad A. Moussa1, Ahmed F. Afify1*, Suzan Salah2 and Ayman Hamed3

... was intended for the antigenic and molecular characterization of betacoronavirus 1 (bovine coronavirus) BCoV in newborn gastroenteritis calves in the Delta Region, Menofia Province, Egypt, also for isolation and identification of the local circulating BCoV strain for further diagnosis or vaccination. In cattle, Betacoronavirus 1 bovine coronavirus (BCoV) is primarily involved in enteric infections which leads to serious complication which may be fatal in youn...
Titin Herawati1,*, Indra Adiwiguna1, Ayi Yustiati1, Iis Rostini1, Roy Hendroko Setyobudi2, Hafizan Juahir3 and Asep Sahidin1
 

...stream and downstream Jatigede Reservoir. Field observation method and census sampling technique were applied. Research commenced from November 2017 to January 2018. Parameters measured are diversity index, dominance index, equitability index, and water quality. Data analysis employed comparative-descriptive method by mapping the species and comparing the diversity of fish living in both ends of Jatigede Dam. The results ind...
Haris Setiawan1, Aris Winaya1,*, Herwintono Herwintono1, Mulyoto Pangestu2 and Yayuk Kholifah3
 

...imousin bull (Bos primigenius f. taurus [Linnaeus, 1758]) collected from Singosari Artificial Insemination Center, Indonesia and commercial SFE. Semen from the same bull were collected and processed according to the protocol at Singosari AI Centre. Semen extender were supplemented with SFE, G0 = 0 % SFE, G1 = 2 % SFE, G2 = 4 % SFE, G3 = 6 % SFE and G4 = 8 % SFE. Each of the treatments was repeated three times. The sperm mortality and motility datafrom S...

Uwem E. George1, Oluwadamilola A. Arowolo2, Oluseyi A. Olayinka3, Ijeoma M. Ifeorah4, Temitope O.C. Faleye3,5, Bolaji Oluremi6, Arthur O. Oragwa7, Ewean C. Omoruyi8, Emmanuel E. Udoh9, Oluwadamilola G. Osasona1,10, Emmanuel Donbraye11, Olufunke P. Adeniji12, Olubusuyi M. Adewumi3,13* and Johnson A. Adeniji3,13,14

...nes (ARGs). We examined Nigerian Chimpanzees in Boki Afi Mountain Wildlife Sanctuary, Cross-River State, Nigeria, to detect ARGs. Faecal samples from 15 Chimpanzees in pristine enclosures of Non-Human Primates in the Wildlife Sanctuary were analyzed. All faecal samples were pooled, then resuspended in phosphate-buffered saline. Subsequently, nucleic acid was extracted from the suspension and Illumina sequencing performed. AR...
Wahyu Widodo1,*, Imbang Dwi Rahayu2, Adi Sutanto3, Apriliana Devi Anggraini4, Harum Sahara4
Sara Safitri4 and Abubakar Yaro5,6
...ter, and organic matter digestibility in super kampong chicken. The best treatment was curcuma by 1 % as feed additive due to the increase of carcass weight percentage by the value was 69.60 %.
...

Oluseyi A. Olayinka1, Temitope O.C. Faleye2, Oladipo O. Omotosho3, Oladapo A. Odukaye3, Bolaji Oluremi4, Ibipeju H. Ibitoye1, Oludayo O. Ope-Ewe1, Uwem E. George5, Oluwadamilola A. Arowolo6, Ijeoma M. Ifeorah7, Ewean C. Omoruyi8, Emmanuel Donbraye9, Olufunke P. Adeniji10, Olubusuyi M. Adewumi1,11* and Johnson A. Adeniji1,11,12

...y in Ibadan, South-West Nigeria. Three fecal pellets were randomly picked from the floor of unit pens containing a minimum of three pigs per unit in selected piggery in Ibadan, Nigeria. The samples were pooled and resuspended in phosphate buffered saline. The suspension was then subjected to nucleic acid extraction, cDNA synthesis and Illumina sequencing. Antibiotic Resistance Genes (ARGs) in the raw reads were determined an...

Salma Javed and Samreen Khan*

...ysporum and Aspergillus niger were examined for their aptitude to assist the population rate of the said species. Nematodes were collected from soil around the rhizospheres of sponge gourd (Luffa cylindrical L.) field in the vicinity of National Nematological Research Centre, University of Karachi and further processed by Cobb’s sieving and Baermann’s funnel techniques. Specimens were killed, fixed and identified up to species level under light mic...

Fazal Wahid1, Arif Ullah Shah1, Muhammad Rahim2*, Fazal Dad1, Nadir Khan1, Saqib Ullah1, Yasir Ali1 and Sayed Ashfaq Ali Shah3

...ted soil samples, a wet digestion procedure was adopted. The targeted metals in selected soil samples were in the ranges of: 1.37-2.00, 28.91-29.50, 1.62-1.82, 1.22-1.179 and 0.07-0.112 mg/kg for Cr, Fe, Ni, Pb and Co, respectively. Increasing order of these heavy metals in soil samples were found as: Plum’s farm soil Co < Cr< Ni < Pb < Fe, Peach’s farm soil as Co < Cr < Pb < Ni < Fe, Tomato’s farm soil as Co < Pb &...

Shujaa Arshad1, Muhammad Umair1*, Rana Shahzad Noor1, Chaudhry Arslan2, Arslan Afzal1 and Zafar Islam3

Jen-Hong Chu1, Ya-Wen Lan2, Shyn-Shin Sheen3 and Angela Chien3*
... muscle composition and digestive enzyme of 0.28 g Laevistrombus canarium. Five semi-purified diets containing 0% (L0), 2% (L2), 4% (L4), 6% (L6), and 12% (L12) lipids were formulated. Each diet was randomly assigned to three replicate groups of L. canarium larvae. The final weight, weight gain percentage and specific growth rate of L. canarium larvae fed with diets L6 and L12 were significantly higher than those of larvae fed with diets L...

Hafiz Basheer Ahmad1*, Sajida Habib2, Waqas Shafqat Chattha3, Rizwana Qamar2, Shazia Saeed2, Abdul Khaliq1 and Salsabeel Rauf2

...lines showed that first eigen value equals 2.77 and represented 39.57 % of the total variability while second eigenvalue equals 1.24 and showed 17.76% of the total variability. The first two factors showed 57.34% of the initial variability. The principal component analysis for R-lines showed that the first eigenvalue equals 3.73 and represented 53.21% of the total variability while second ...

Muhammad Shakil Ahmad1*, Muhammad Afzal1, Sylvain Nafiba Ouedraogo2 and Muhammad Zeeshan Majeed1

...ce are recommended to indigenous potato growers for commercial cultivation in areas where S. litura threatens potato crop.

...

Khan Tamoor1*, Hafsa1 and Hanif Maryam2

...idal property of four indigenous medicinal plants viz., Ferula oopoda, Zataria multiflora, Achillea santolina and Nepeta cateria against Meloidogyne incognita at banana orchards of District Lasbela, Balochistan, Pakistan. Crude extracts, essential oils and fixed oil of these selected plants were tested separately. The crude extracts of Z. multiflora and F. oopoda showed higher mortality as compared to A. santolina and N. cateria. The results showed a maximum m...

Muhammad Waqas1*, Muhammad Shoaib2, Muhammad Saifullah1, Adila Naseem4, Sarfraz Hashim1, Farrukh Ehsan1, Irfan Ali3 and Alamgir Khan1

...dy, the Artificial Intelligence (AI) based techniques: TB (Tree Boost), DTF Decision Tree Forest, SDT Single Decision Tree and conventional Multilayer Perceptron Neural Networks (MLPNN) are used for predicting streamflow of Jhelum River basin. The dataset was divided into two sections, i.e., training dataset (1971-2000); and testing dataset (2001-12). The tendency investigation was done by the Sen’s slope and Mann–Kendall (MK). Decreasing trends an...

Muhammad Irfan Ullah1*, Sana Majeed1, Muhammad Arshad1, Nimra Altaf1, Muhammad Luqman2, Asad Abdullah1 and Muhammad Afzal3

...inga oleifera L. on the digestibility indices and survival of Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae). The P. hysterophorus extract at 50 mg concentration was comparable to the alone application of emamectin benzoate in terms of reduced digestibility and survival of S. litura. Overall, 42.7% relative consumption rate (RCR), 74.3% efficiency of conversion of ingested food (ECI), 72.3% efficiency of conversion o...

Rahat Rukhsana1, Shahnaz Rashid2, Asma Fatima2, Owais Iqbal Khan3, Syed Babar Hussain Shah4, Mumtaz Ali4 and Ghulam Abbas2*

... phytoplankton-density. Eigenvalues of the first two principal components represented 99.97 % of the total variability in water quality of fish and shrimp ponds at different farms. Positive loadings indicated high contribution of water quality variables throughout the study period.

...

Gulnaz Parveen1*, Naila Mukhtar2, Shumaila Irum3 and Nain Bukhari4

...pus, Mucor, Aspergillus niger, Aspergillus flavus, Cladosporium, Botrytis cinerea, Fusarium moniliforme, Drechslera, Penicillium, and Geotrichum candidum were identified among various vegetables including potato, carrot, okra, eggplant, turnip, cucumber, round gourd, cauliflower, and chilli. These losses result in about a 30% reduction in the yield of these vegetables. By reducing the post-harvest losses, it could be possible to overcome the need of food as th...

Mohammed Bashar Danlami1*, Basiru Aliyu1, Abbas Bazata Yusuf1, Bashar Gulumbe Haruna1 and Asmau Nono Mahe1

Iftikhar Jan* and Sahib Alam

... in search for nonaflatoxigenic strains for use as biocontrol agent. A total of 434 isolates of A. flavus were isolated from soil and stored maize grain samples collected from four agroecological zones (AEZ) i.e., AEZ-A, AEZ-B, AEZ-C and AEZ-D of Khyber Pakhtunkhwa (KP) province of Pakistan. The mycological analysis of the samples demonstrated that the highest population of Aspergillus section Flavi was existed in soil (n = 236) than in maize grain samples (n ...

Syed Muhammad Sulaiman, Nazir Ahmad and Nazir Ahmad Khan*

...e nutritional value and digestibility among five selected wheat genotypes. The five cultivars, namely, Pir Sabaq (PS)-15, Bakhtawar-92, PS-2000, National Agriculture Research Council (NARC)-11 and Zam-04 were grown in 12 replicate plots (6 m × 10 m), that were blocked in three fields according to Randomized Complete Block Design. Uniform field preparation, irrigation, weeds control and standard agronomic practices were applied to all plots. For forage yi...
Ying Yang 1,2,3, Tietao Zhang 1,2,3, Min Rong 1,2,3, JiaPing Xu 1,2,3 and Xiumei Xing 1,2,3*
...l content. The apparent digestibility was digested better by male than by female. The energy levels affected the performances of female mink to a lesser degree than those of male mink. Diets containing energy at levels below 15.06KJ/kg and exceed 29.06 KJ/kg of dry matter did not support optimum growth of male, but energy levels at least as low as 17.81KJ/kg and no more than 29.06 KJ/kg appeared to be satisfactory for the gr...

Ain-ul-Abad Syed1*, Zaheer Ahmed Khan1, Shakeel Hussain Chattha1, Irfan Ahmed Shaikh2, Mian Noor Hussain Asghar Ali1, Zohaib ur Rehman Bughio3, Shahzad Hussain Dahri2 and Ghous Bakhsh Buriro4

...ors and artificial intelligence technologies to handle difficulties related with food supplies. The hydroponic growing technology should be introduced to cities. At the time, such as when challenged with Covid-19 and the locust attack.

...

El-Habbaa, A.S.

...d chicken eggs (ECE), antigen detection using agar gel
immune-diffusion test (AGIDT) and indirect-Immunofluorescence assay (IFA) and
molecular detection of viral DNA using PCR. Positive results were showed in 36 of
samples (28/30 of sheep samples and 8/10 of goat samples)upon isolation on ECE by the
3rd passage and 22 of samples (17/28 of sheep isolates and 5/8 of goat isolates) using
AGIDT and indire...
Abeer A. Feisall; B.A. Othman2; Kh.A. El-Dougdoug2 and H.S. Shalaby1
...div>
resistant to digestion with PvuI, BamHI, XhoI and EcoRI. The genome size of the
phage was estimated to be 18 kbp.
...

Enas K. Abo-Elmagd,Kouka S. Abd El-Wahab,Azza H. El- Salakawy

...t for detection of RV antigen in stool specimens collected from Egyptian infants and young children with ELISA, and nested reverse transcription polymerase chain reaction (RT-PCR). Furthermore, to study the frequency of RV infection in Egyptian infants and young children during the summer season 2012 and the effect of certain risk factors including age and gender on the extent and impact of RV infection. The study included 73 infants and young children attendi...
Nader M. Sobhy,1 Sunil K. Mor,2 Mohammed E.M. Mohammed,1
Iman M. Bastawecy,3 Hiam M. Fakhry,4 and Sagar M. Goyal2
...ntial for genetic and antigenic variation. Therefore molecular study of FMDV is pre-requisite for amplification of FMDV specific genomic fragment and also copying specific serotypes O, A and SAT2 nucleotide sequence. In this work FMDV serotype O, A and SAT2 were isolated from cattle clinical samples (foot lesions). RNA extracted from clinical samples was subjected to reverse transcriptase polymerase chain reaction( Rt. PCR) nucleotide of FMDV ID and 3D genes w...

Ebtisam, A. Abouel yazeed1; Yanni, M.I.1 and Hanan A. Fahmy2

...gate bovine rotavirus antigen in fecal samples of forty diarrheic new borne calves of cattle (32) and buffaloes (8) below three months in some farms in Egypt. The detection was done by ELISA and indirect fluorescent antibody technique (FAT) as well as molecular method as real-time quantitative reverse transcriptase PCR (RT-qPCR) from samples collected during neonatal diarrhea. Six out of forty tested samples were found positive for rotavirus (15%) infection us...

Hanaa A. Ahmed1 and Eman M. AboHatab2

...curate method for LSD antigen detection. nodules were subjected directly to PCR and Real time PCR for rapid and specific detection of LSDV, sixteen out of the twenty one nodular samples were confirmed positive by molecular methods. Eleven out of twenty one nodular samples were positive by (IFAT) in CAM, which prove that PCR and Real Time PCR are much sensitive and rapid diagnostic tool of LSD reflecting their importance in controlling the rapid spread of disea...

Afaf. A. Khedr*, Ekram Salama*, A. A. Samy**, Abdella, Y.A.*** and Suzan. K. Tolba***

...mune response for the antigenic component of both vaccines show high titre of antibodies, but Montanide ISA-70 adjuvanted vaccine was superior as shown in mean antibody titers against Avibacterium paragallinarum serovar A, B and C and infectious bronchitis. In addition, Montanide ISA-70 is lesser in viscosity that makes it easily injected, so, it is better to use for control of infectious coryza and infectious bronchitis diseases in chickens.

...

Hala K. Abdelmegeed 1, Eman M. Abohatab 1, Khattab,O. M. 1 , Salem, S.A.H. 1 , Arafa, A. 2, Nashwa M. Helmy 3

...ISA kit used for FMDV antigen detection for the epithelial suspension from 42 field samples collected from various geographic locations 10 governorates are ( El-Sharkia,Giza, Port Said, Assuit, Suize, Kafre El-Shake, Qina, Al-Gahrbia, Domiatte and Alexandria) Serotyping and molecular characterization by polymerase chain reaction PCR for (31) samples as FMD Serotype O , (2) Serotype A and (3) serotype SAT2 the samples collected from outbreaks in period November...

Nashwa M. Helmy1 and Ahmed S. A.2

...he detection of virus antigen or
genome. So, the vesicular epithelial tissues collected from infected cattle from Sharkia
(7) and Fayoum (5) governorates were subjected to antigen ELISA and RT-PCR. 8
epithelial tissues were identified by Indirect sandwich ELISA. The results were 5 and 3
positive from Sharkia and Fayoum respectively, while 12 samples were positive by RTPCR.

Serag eldeen Sultana, M.W Abdel azeemb, Nahla Mohamed Eldamranyc

...est against H5 and H9 antigens. The overall results using both ELISA and HI tests revealed that the prevalence of AIV antibodies was 12.6% (18/143). The seropositive samples represented 5/143 and 15/108 for ELISA and HI, respectively. The detection of both H5 and H9 antibodies in (4) samples indicated that co-infection may occur, while (10) samples were only containing H9 antibodies and (1) sample was H5 positive by HI test. The AIV positive samples were detec...

Aya El-Turkey1, A. K. El-Attar1, A. E. Aboulata1, B. Othman2 and K. A. El-dougdoug2

...ion of the introduced antigen construct at the RNA level was detected by RT-PCR, using specific primers for the HSV-gD2 transcript. Expression of the HSV-2 gD antigen was assayed by ELISA on protein extracts isolated from same transfected plant tissue samples as for the RT-PCR assays. Results showed that the HSV-gD2 construct was expressed at both the RNA and protein levels in the transgenic tomato plants. Finally, we report...

K.A. El-Dougdoug1, A.R. Sofy2, A.A. Mousa2 and E.E. Refaey2

...
tuberosum subsp. andigenum
...

Aly M. Abdel-Salam

... of BSV captured SCBV antigens in leaves and insect vectors in
Immunocapture (IC) PCR analysis. The present study represents the first record of
SCBV presence in Egypt.
...

Elharony, S.B.1,3, SaharA.Youssef2 and Richard F. Lee3

...ues such as restriction digestion,
Southern blotting and cloning.
...
M. A. Kararah1, Om-Hashem M. El-Banna1, Salwa N. Zein2 and Abd-Elrehiem, A.F.3 
...00, respectively. The antigen dilution end point was
1:500.The produced antiserum was evaluated by ELISA and DBIA. Electron
microscopy f ultrathin-section of PMV- infected leaf tissue revaled several
morphological changes and aggregated virus particles associated with
pinwheel inclusion bodies. Salicylic acid and Parahydroxy benzoic acid
were used for induction of systemic acquired resistance, whereas...

Sherif .M. Ibrahim*, Abd El-Razek. B. Abd El-Razek*. Hanan. M. El-Zahed Amal. A. Fatouh* and Ayatollah. I. Ibrahim*

...owl pox virus (FPV) and Pigeon pox virus (PPV) based on biological and molecular basis. Vaccinal and isolated strains were propagated on the chorioallentioc membrane of specific pathogen free (SPF) embroynated chicken egg (ECE) showing the characteristic pock lesions for each strain with titers reached 4.5 and 3.5 log10 EID50/ml at 5th passage for FPV and PPV isolates, respectively. The isolated strains were further identified using the specific hyper-immune s...

Sahar Abd El Rahman1; Mohamed Eladl2 and Mohamed M. El-Diasty3

...acterize of the viral antigens, then molecular characterization of its genome. Infection by the bovine ephemeral fever virus (BEFV) was noticed in Dakahlyia governorates during the summer season of 2015. Twelve samples of buffy coats were collected from dairy farms suspected from clinical investigations to be infected by bovine ephemeral fever virus. The virus was isolated intracerebrally in suckling mice then successfully identified by indirect immunofluoresc...
Abd El-Hamid, M.I1, Seham, A.El-Zeedy1, El-Sanousi, A.A2, Reda, I.M2, Nehal, S. Saleh1, Abbas, A.M1
...cturing of diagnostic Antigen for detection of the circulating antibody either in vaccinated or latent infected horses.

...

Lala Rukh1, Muhammad Nadeem1, Tusneem Kausar1, Mian Anjum Murtaza1, Muhammad Luqman2*, Muhammad Bilal Shahid1 and Amal Shaukat3

...itro starch and protein digestibility, antioxidants and microbial count. Results showed that date bars are rich source of protein, fiber, carbohydrates and fat with good digestibility properties. Date pit powder and soy protein isolate incorporation enhanced the total antioxidants and DPPH activity in soy date bars however, decreasing trend was observed in total antioxidants and inhibition of DPPH radical activity during 90 ...

Ehab M. El-Nahas1, Hemat S. El-Sayed2, Sawsan S. El-Basuni3, Gabr F. El-Bagoury1

...h further genetic and antigenic characterization. Methods: Beside a real time RT-PCR; sequence analysis of partial S1 gene, cross-neutralization and the challenge experiment for H120 vaccinated broiler chickens were also performed. Results: Egypt/Qal/014p had close relation with that of the Massachusetts prototypes. Comparisons nucleotide and amino acid sequence of partial S1 gene showed that the recent isolate had 98.2% and 95.2% nucleotide similarities and 9...

Ayman S. El-Habbaa 1, Gabr F. El-Bagoury1, Samar F. El-Adaway2, and Susan S. El-Mahdy2

...urther studies on the antigenic characters of that variant isolate is required to study antigenic variation between NDV strains circulating in Egypt.

...

Naglaa F.S. Awad1, Gamelat K.F. Kotb2

...Molecular analysis of antigenic
regions of VP60 (part of E and F regions) revealed neither detection of recombination points nor sites
under positive selection.
Conclusion: The obtained results indicate that non-hemagglutinating RHDVa G6 variant viruses are
currently circulating in Sharkia with the ability to infect both young and adult rabbits that may be due
to inadequate application of vaccine and ...
Maha M. Khedr1, Reem A. Suliman1, Marwa F. Mohamed1, Mounir D. El Safty1, Hussein A.
Hussein2
...>Also, the 300 HAU of antigen in the prepared avian influenza H5N1 vaccine are not enough in reducing
of virus shedding post challenge.
...
Hussein A. Hussein, Maha Khedr, Reem A. Suliman, Marwa F. Mohamed, Mounir D. El
Safty
...nation of the optimal antigen content of avian influenza virus
vaccines is urgent to reach protective antibody titers and reduce virus shedding.
Methods: Groups of one-day old commercial broiler chicks were divided in to 8 groups 1, 2 and 3 were
vaccinated with a prepared vaccine contain 500HAU of H5N1 reassortant antigen; while group 4, 5 and 6
were vaccinated with an imported...
Dalia M. Omar1, Nermeen A. Marden1, Lamiaa M. Gaafar1, Elham A. El-ebiary1, Khalid El-Dougdoug2, Badawi Othman2, Abd Elsattar Arafa3, Hussein A. Hussein4
...entified and subtyped antigenically by serological test as
Haemagglutination inhibition (HI) using standard AI antisera for H5 antigen and genetically by RTPCR
using specific primer for H5 gene and it was confirmed to be H5N1 and grouped according to the
year of isolation as 2016 isolate strain.
Results: According to the phylogenetic analysis it falls into the classical group o...

Nermeen A. Marden1, Lamiaa M. Gaafar1, Hussein A. Hussein2

...ed that sufficient HA antigen content and similar HA sequences
with the field HPAI challenge viruses are needed to produce a consistent and high protection percent,
but, they are not enough to assess the vaccine efficacy.
Conclusion: The invitro assessment of protection was not considered an alternative methods to the
direct assessment of vaccine efficacy (vaccination-challenge), but it was used as complementary ...
Asma Kaouachi1, Mohcen Menaa1*, Abderraouf Chouaib Rebbah2 and Mohamed Cherif Maazi1
...-size: small;">The wood pigeon Columba palumbus is a common bird in Algerian forests. In the near past the species populations have increased significantly in Algeria. In order to better comprehend the ecology and vital needs of the wood pigeon and to help with our results in the process of conservation management of wood pigeon, a study of the diet of this p
Saira Gul1,2, Sohail Ahmed2*, Tahir Usman1*, Khalid Khan3, Sultan Ayaz1, Saleha Gul4 and Nawab Ali5
...ed to the younger and indigenous cattle breeds, respectively (P˂0.01). Moreover, the study revealed widespread prevalence of A. marginale in the subtropical areas of KP, Pakistan.
...

Akram I. Aboelkhair1, Ayman H. El-Deeb2, Momtaz A. Shahin1 and Hussein A. Hussein2

...onventional PCR . For antigenic identification SNT were
applied. Sequencing of positive sample reveled identity of LSDV by targeting G-protein coupled
chemokine receptor gene (GPCR) which confirm that there is no genetic changes in LSDV.
Conclusion: phylogenetic analysis of LSDV at summer 2017 revealed that recent isolates has no
genetic difference compared by other isolates from Egypt or Africa during previous f...

Hanaa A. Elsamadony1, Laila A. Tantawy1, Sabry E. Omar2 and Heba A. Abd Alah1

...etect CAV
antigens by immunohistochemically in thymus and bone marrow tissues of SPF chicks infected with
CAV.
Methods: In this study, 30 layer farms of age 40 to 100-days-old from different governorates
(Alexandria, Gharbia, Giza and Qalubia) were examined for CAV with real-time PCR and
histopathological examination. The positive samples were prepared and inoculated in SPF chicks' oneday-

Mohamed F. ElKersh1, Fatma M. Abdalla2, Gamilat K. Farg2, Soad A. Nasef3, Ahmed A. Ali2

... from Chicken, Duck and pigeon, and AIV H9 strain that isolated from chicken show where mutated strains from previously AIV circulating in Egypt from 2006 to 2008. Therefore, we recommend incorporation of these such isolates in any newly prepared AIV vaccines and vaccination program.
...

Tahsin S. Shoala1, Ahmed K. El-Attar2, Ahmed I. Abd El Maksoud3

...iv>and cultured in Murashige and Skoog media (MS media). Four weeks later, potato plants were
tested for PLRV using RT-PCR technique, and all of the plants were PLRV positive. MS
media were prepared, autoclaved, and active natural materials (Glycyrrhizic acid ammonium
salt (GAS), Glycyrrhizic Acid (GA), Curcumin (CU) and Rosmarinic Acid (RA) were
sterilized and added to MS media at different concentrations.
...

Wesley D. Nafarnda, Japhet H. Baraya, Olatunde H. Olabode, Malang K. Simon

...l poultry especially in Nigeria.
Aim: This cross sectional study was conducted to establish the impact of V4 thermostable ND
vaccination on rural poultry productivity using corn bran (dusa) as alternative vaccine carrier in five
Local Government Areas (LGA) in Adamawa state, Nigeria.
Method: Structured questionnaire on general knowledge, practice and productivity indicators was...
Karim M. Selim1, Abdullah A. Selim1, abdelsattar Arafa1, Hussein A. Hussein2, Ahmed A. El-Sanousi2
...ing GVII monospecific antigen and decreased virus shedding
than other group. The vaccinated group with genotype VII significantly reduced the virus
shedding at 3, 5, 7 and 10 days post challenge unlike the genotype II vaccinated group that
did not reduce virus shedding and increased morbidity and mortality in contact birds.
Conclusion: The combined inactivated and live NDV vaccination programs have the
Song Jiang1,2,3, Xianbin Mo1,2, Falin Zhou2,3, Jianhua Huang2,3, Qibin Yang2,3, Lishi Yang2,3 and Shigui Jiang2,3*
Atta Muhammad Memon1, Ubedullah Kaka2*, Muhammad Umer3, Asghar Ali Kamboh4, Atique Ahmed Behan5, Habibullah Janyaro6, Yong Meng Goh7, Amir Bukhsh Kalhoro6Jalila Abu8, Khaleeq ur Rehman Bhutto9 and Arsalan Maqbool10


...ketamine anaesthesia in pigeons. Twelve pigeons were administered with 1) medetomidine as a pre-anaesthetic at 120 µg/Kg, followed 10 min later by ketamine at 30 mg/Kg (MK group) and 2) Medetomidine and ketamine followed by atipamezole (MKA group). Medetomidine produced moderate sedation within 3.08 ± 0.21 min. Medetomidine–ketamine produced smooth and excitement free induction of anaesthesia in p
Muhammad Mobashar1, Qazi Aqeel1, Muhammad Tahir Khan1, Assar Ali Shah2*, Ahmed A.A. Abdel-Wareth3, Salman Khan4, Nazir Ahmad1, Sami Ullah1 and  Haq Amanullah5
...In-vivo nutrient digestibility, dry matter, organic matter, crude protein, crude fat, nitrogen-free extract (NFE), ash were significantly higher (P<0.05) in treatment groups, while in in-sacco degradability the DM and OM degradability at different time intervals (2h, 4h, 8h, 12h, 18h and 24 h) were significantly (P<0.05) higher in ration C and D. The initial body weight was significantly improved in treatment groups compared to control, whi...
Zhengyi Fu1,2,3,4, Rui Yang2,3,4, Zhenhua Ma2,3,4*, Mingyang Han2,3,4 and Yifu Wang2,3,4
...rameters, histology and digestive enzymatic activity of barramundi (Lates calcarifer). Fish were fed ioso engery diets (18 kJ/g) with two types of non-protein energy sources in the experimental groups and a regular diet was used as the control. The feeding trial lasted for 56 days. Results from the present study indicate that, except for weight gain and feed intake, most growth parameters of fish were not significantly affected by the experimental diets...
Sen Liu1,2, Yue Zhu2, Haixia Leng2, Ying Wang1, Xiangju Yin1 and Yajun Zhao1*
.... In previous studies, epigenetic variation in the muscles of bat populations were explored; however, the extent of the patterns and levels of genomic DNA methylation among tissues in chiropteran animals have yet to be investigated. In this study, the epigenetic variation of six tissues in normal state Rhinolophus ferrumequinum (i.e., no pregnancy, nursing, or hibernation) was explored using the F-MSAP technique, incl...

Jo Ik-Hwan1, Choi Kwang-Won1, Muhammad Yaqoob2 and Muhammad Fiaz3*

... levels. Yield of total digestible nutrients (TDN) was highest at 150 kg N/ha in triticale sole monocrop, followed by 100, 50 and 0 kg N/ha levels. The overall TDN yield in triticale vetch mixture with 100 kg N/ha was higher (P<0.05) than control and not different (P>0.05) from 50 and 150 kg N/ha levels. However, TDN yield of triticale-pea mixture was greater (P<0.05) than triticale monocrop. Carrying capacity for Hanwoo cows in triticale with vetch a...
Aqsa Shahid1, Saima Muzammil1, Farhan Rasheed2,Bilal Aslam1, Muhammad Akhtar Ali3, Syed Zohaib Haider4, Masroor Ellahi Babar4, Muhammad Saeed1, Umair Waqas1 and Mohsin Khurshid1,*
...es whereas colistin and tigecycline were the most active antimicrobial agents against A. baumannii as none of the isolates was found resistant to these two drugs. Moreover, the armA was found in 25% (n=37) of A. baumannii isolates which showed a high level of resistance to aminoglycosides i.e., amikacin; ≥256µg/ml (Breakpoints; ≥64µg/ml) and gentamicin; ≥MIC 64µg/ml (Breakpoints; ≥16µg/ml). This study ha...
Diana Rachmawati1*, Johannes Hutabarat1, Olga Anne2, Roy Hendroko Setyobudi3 and Tita Elfitasari1
...aculture engineering of tiger grouper (Epinephelus fuscoguttatus Forsskål, 1775) on Bacillus subtilis supplementation in the diet is one of the solutions to overcome the deterioration of the aquaculture environment caused by the accumulation of dieting waste. The purpose of the research was to study the effects of B. subtilis supplementation in the diet on protein digestibility, the efficiency of d...
Muhammad Bilal Tayyab1, Muhammad Zeeshan Majeed1*, Muhammad Asam Riaz1, Muhammad Anjum Aqueel2, Sylvain Nafiba Ouedraogo3, Muhammad Luqman4, Kanwer Shahzad Ahmed1 and Mujahid Tanvir1
...cetone extracts of 40 indigenous plant species of Soon valley and surrounding salt range (Punjab, Pakistan) against D. citri and A. gossypii using standard twig-dip and leaf-dip bioassay methods, respectively. Results of initial screening bioassay showed the highest mortality of D. citri by 10% extracts of Mentha longifolia (L.) Huds. (93%), Melilotus officinalis (L.) Pall. (91%), Nerium indicum Mill. (89%), Datura alba L. (88%) and Salvia officinalis L. (81%)...
Ahmad Wahyudi1*, Sujono Sujono2, Listiari Hendraningsih2, Ari Prima2, Zane Vincēviča-Gaile3 and 
Ivar Zekker4
...ed were feed intake and digestibility of dry matter (DM) and organic matter (OM) and average daily gain (ADG). The data were analyzed using Analysis of Variance (ANOVA) and followed by Least Significant Difference (LSD) test. The average DM intake (kg head-1 day-1) for CON, TMR, and TMR silages were 5.15; 6.79; and 4.14 meanwhile OM intakes were 4.68; 6.04; and 3.94 respectively, TMR significantly higher than others (P < 0.05). ...
Mona Mohammed EL-Derbawy1, Eman Naser Hafez2*, 
Mona Abdel Daym Abd Rabbo1, Nagwa Ibrahim Toaleb3,
 Saedia Abdel Hady Sayed El-Ahl1 and Bahaa Eldkeen Wade EL-Aswad4 
...mansoni cercarial antigens (SCA) by ELISA for serodiagnosis. Total IgG ELISA was assessed to evaluate the potency of two parts of Schistosoma mansoni ceracrial antigens (2000 each) one normal and other exposed to attenuating dose of 400 Gy gamma radiations. The study was conducted on fifty two individual sera samples divided as follows: Group І included 21 obtained from patients who were positive for schistosomia...
Muhammad Javed Iqbal1,*, Muhammad Afzal1, Khalid Abbas1 and Muhammad Shahid2
... nutrients and minerals digestibility of Labeo rohita fingerlings. Twelve experiment diets containing four protein levels (24, 26, 28, and 30 %) at three dietary energy levels (2400, 2700, and 3000 kcal/kg) with P/E ratios from 80.00 to 125.00 mg / kcal were evaluated. Each diet was randomly assigned to triplicate groups of seventeen fish averaging 6.5±0.6 g (m...

Mukhtar Iderawumi Abdulraheem1*, Muhammad Ihtisham2*, Abiodun Yusuff Moshood3, Nawab Khan4, Muhammad Owais Shahid5, Shafiq Hussain6, Kumail Abbas6 and Fawad Zaman7

...rtant vegetable crop in Nigeria where viruses cause severe yield loss due to tropical climate. This study will help in the production of disease-free okra crop without chemicals application. Moreover, our study will contribute to the organic production of this important vegetable crop.

...

Kemi Funmilayo Omotesho1*, Philip Akintunde Fatodu1 and Toyin Benedict Ajibade2

...eeping among farmers in Nigeria greatly impedes the evaluation of the performance of farms, national planning, budgeting for commercial agricultural development, and control of postharvest losses. The study analysed the record keeping behavior of youth farmers in Ekiti State. Specifically, it assessed the level of record keeping among farmers; examined their attitude towards record keeping; assessed the farmers’ knowledge of record keeping; determined th...

Theophilus Miebi Gbigbi* and Rodney Akpoviri Isiorhovoja

...rprises in Delta State, Nigeria. A multistage sampling procedure was adopted to pick a sample of 120 farmers from whom data were elicited using structured questionnaire. Data were analyzed using descriptive and inferential statistics. The findings indicate that the average age of respondents was 43 years; majority (85%) of respondents were males with 16 years’ experience in exotic chicken production; 75% were married and 48% had secondary education. The ...

Saadu Mustapha1,2, Norsida Man1*,  Jasmin Arif Shah1, Nitty Hirawaty Kamarulzaman1 and Ahmadu Abubakar Tafida3

Yonis Abukar Mohamed1, Shafii Abdullahi Mohamed1,2, Abdiaziz Idiris Mohamud1,3*, Abdiaziz Ahmed Mohamud1,3, Kassim Abdullahi Jimale1,2 and Said Ali Ibrahim2

...ems such as depression, digestive problems, respiratory problems, improper harnessing, ocular, hoof overgrowth and fracture, respectively. Additionally, 40.6% of the examined donkeys had varying degrees of lameness. 54.6% of the examined donkeys, were with either healed or active wounds (skin lesions), 17.4% scars, and 28.0% both skin lesions and scars. Moreover, dermatological diseases were also encountered, habronemiasis 59.1%, ectoparasite 35.7%, Sarcoid 5....

Mst. Deloara Begum1, Md. Muniruzzaman1, Md. Salauddin2* and Md. Mostafizer Rahman1

...onella spp.12.5% (21), Shigella spp. 8.93% (15) and Klebsiella spp. 2.97% (5). In cooked fish 9 isolates were identified and species were Escherichia coli 66.66% (6) and Shigella spp. 33.34% (3). Total viable count varies from 1.28×107CFU/g to 3.74×109CFU/g. The highest concentration was found in Loitta fish and lowest in Ruhi from dried fish. Most species of bacteria isolated were resistant to amoxicillin, penic...
Vishal Ambidi 1,3, Sanjeev Bantewad2, Suraj Prasad Mishra1, Anupama Hingane1 and Jagdish Jaba1*
...borer, Helicoverpa armigera and other pod borer complex. Among pod borer, which is the most devastating pest of pigeonpea. An understanding of different morphological and biochemical components of resistance is essential for developing strategies to breed for resistance to insect pests. morphological and biochemical components associated with expression of resistance to pod borer complex in Pi...
Muhammad Hamza Tariq1, Mahjabeen Akram2 and Yasir Sharif3*
...amely Morin, Curcumin, Apigenin, Cedronolactone A and Matairesinol showed acceptable drug-like properties therefore these compounds can be proposed as effective inhibitors, disrupting the S-protein- ACE2 interaction. This study might help in the development of a natural and cost-effective drug against COVID-19. Further, in vivo and in vitro examinations are required to validate our results.
...
Sohail Anjum1, Awais Ahmad1, Farzana Bibi1 and Hazrat Ali2*
...n abundance. Their intelligence is also acknowledged duly. They are also obligate to human presence. We can assume that “where there is human, there is house crow essentially”. The present article documents the ecology of this bird in Dir Lower, Khyber Pakhtunkhwa, Pakistan. The house crow population has reached a considerable size in the study area. The species has colonized both rural and urban areas and has dispersed throughout the study area. O...

Ruqia Bibi1, Najam-un-Nisa1, Sania Komal1, Hafiza Nimra Ghani Qureshi1, Maryam Safdar1, Hafiza Hina Jameel1, Saman Gul1, Sania Saeed1 and Inam Ullah1,2*

...e Cormorant (Microcarbo niger), Gadwall (Mareca streper), Great Cormorant (Phalacrocorax carbo) were found as the dominant species with the relative abundance of 0.07, 0.07 and 0.06, respectively. As migratory birds indicate a freshwater ecosystem, healthy bird species indicate a healthy habitat, while declining bird populations indicate degraded habitat. Studies concluded that the diversity of migratory birds is decreasing at Chashma Lake due to deforestation...
Nasir Ali Tauqir1, Asim Faraz2*, Muhammad Arif1, Abdul Rehman1Imtiaz Hussain1, Abdul Waheed2, Gabriele Marino3 and Michela Pugliese3

 

...concentrations while CP digestibility remained unchanged. Milk yield and daily weight gain decreased linearly (P<0.01) with reducing dietary levels of EAA concentrations. A positive nitrogen balance was also observed in buffaloes fed all the experimental diets but it was similar statistically. Hematological analysis indicated decreased (P<0.01) mean corpuscular volume and hemoglobin concentrations in buffaloes fed gradual decreased dietary EAA concentrat...
Muhammad Arsalan1, Muhammad Aman Ullah1 and Abdul Waheed2,*
...ere obtained from 291 indigenous sheep found in the southern Punjab of Pakistan. Ten various body indices were obtained from 14 morphological traits. Pearson correlations between live body weight and 13 other morphological traits obtained to analyze the nature and strength of the relationship. Simple regression models were fitted to predict live body weight from 13 other morphological traits as independent variables. Descriptive statistics of body indices and ...
Ho-Seong Cho1 and Yeonsu Oh2,*
... for carcinoembryonic antigen, fetoprotein and hepatocyte paraffin-1. Taken together, the tumor was diagnosed as cholangiocarcinoma. This is the first case report of a cholangiocarcinoma in the puma.
...
Nasir Ali Tauqir1, Asim Faraz2*, Annamaria Passantino3, Muhammad Asif Shahzad1, Rana Muhammad Bilal4, Adeel Tahir1, Hafiz Muhammad Ishaq2 and Abdul Waheed2
...icantly higher nutrient digestibility and carcass characteristic as compared to other treatments. Growth responses, nutrient digestibility and carcass characteristics of birds were optimized fed 3% of CSL and Enzose mixture as compared to others.
...

Nguyenthuy Linh1, Nguyen Thi Kim Dong2*, Nguyen Van Thu3 

...nutrient and amino acid digestibility of local Muscovy ducks in two periods. Two factors of the trial were dietary lysine levels (0.8%, 1.0% and 1.2%) and metabolizable energy (ME) levels (12.55MJ/kg DM and 12.97MJ/kg DM) for 8-week-old period; and dietary lysine levels (0.7%; 0.9%; 1.1%), and ME levels (13.39MJ/kg DM; 13.81 MJ/kg DM) for 10-week-old period. There were three replications and four ducks per experimental unit. The findings indicated that the app...

Pham Tan Nha*, Le Thu Thuy 

...and SH4 treatments were higest (60.3g/kg, 61.3g/kg and 61.2g/kg, respectively). It was concluded that the treatments supplemented with hydrolyzed shrimp juice at 3% and 4% gave higher results in terms of weight gain and low feed conversion ratio.

Keywords | Hoa Lan duck, Shrimp soluble extracts 

...

Nguyen Binh Truong1,2,3*, Nguyen Van Thu3 

... feed intake, nutrients digestibility, rumen parameters and nitrogen retention of crossbred beef cattle (Black Angus × Zebu crossbred). Four male cattle at 17.5±1.78 (Mean±SD) months of age with an average live weight of 262±20.5 kg (Mean±SD) was allocated in a 4x4 Latin square design. The treatments were 47, 51, 55 and 59% NDF in diets corresponding to NDF47, NDF51, NDF55 and NDF59 treatments following the previous findings o...

Qurban Ali1, Muhammad Faheem Akhtar1, Asad Aslam1*, Muhammad Shehzad2, Muhammad Jamal2, Humaira Malik1, Imran Nadeem1, Aqsa Abbas1, Muhammad Jawad Saleem1, Tamsila Nazir1 and Kanwal Hanif1

...od borer (Helicoverpa armigera) is economically vital insect pests for chickpeas and caused huge yield losses in south Asian countries. Host plant resistance, physio-morphic characters counteract the ability of insect to demage and to cause minimum reduction in yield. The experiment was conducted under natural and semi-natural conditions to determine the host plant resistance of nine (09) chick pea advance genotypes viz., (K01211, K01216, K01241, K01242, K0901...

Dewi Ratih Ayu Daning1,2, L.M. Yusiati1, C. Hanim1, B.P. Widyobroto1*

...thane (CH4), dry matter digestibility, and ammonia (NH3) significantly decrease (P<0.05) at all doses of galangal EO. The addition of cineole results in the significant decrease (P<0,05) of CH4 (ml/dry matter degraded) while dry matter degradability and gas production shows a significant increase (P<0.05). Also, the addition of cineole results in a more significant increase (P<0.05) of propionate, acetate, total volatile fatty acids, and NH3 compar...
Yang Yang1,2, Ji Shao1,2, Mingxu Zhou3,4, Qiangde Duan1,2, Xinyi Zhang1,2 and Guoqiang Zhu1,2*

...ustry, while F4 enterotoxigenic Escherichia coli (ETEC) is an important pathogen causing diarrhea of newborn or weaned piglets, which seriously harms animal husbandry and causes huge economic losses. The pathogenicity of E. coli is closely related to its virulence factors, which are strictly regulated in vivo, and the quorum sensing system is involved in this process. To study the effect of quorum sensing signal molecule acyl homoserine la...

Ahmed M. Elbaz*, Engy F. Zaki, Morsy A. S. 

...atio, and crude protein digestibility of broiler chickens. Including quinoa seeds significantly increased carcass dressing and decreased ( P < 0.05) abdominal fat content. Plasma triglyceride, LDL, creatinine, and uric acid levels were lower (P<0.05) in the birds fed QS supplementation, while HDL was significantly higher. Saturated fatty acid content and shear force value (more tender) were reduced (P<0.05) while increasing the levels of quinoa inclus...

Muhammad Rizwan1*, Jehanzeb Farooq1, Amjad Farooq1, Muhammad Farooq1,2, Ghulam Sarwar1, Muhammad Nadeem1, Muhammad Riaz4, Muhammad Rafique Shahid3, Hafiz Ghazanfar Abbas1 and Muhammad Kashif Shahzad Sarwar1

...02 out of 08 PCs showed eigen value greater than 1. The contribution of first two PCs in total variability was 70.77% amongst the genotypes assessed for chlorophyll contents, seed cotton yield and related traits. Remaining six components contributed very less viz. 30.8% in total variability. Valuable positive factor loadings on PC I was attributed by seed cotton yield, chlorophyll contents, boll weight, No. of sympodia, seed index and Ginning out turn (GOT)%. ...
Shoaib Ahmed Pirzado1,2*, Wu Zhengke1, Adanan Purba1, Chen Jiang1, Huiyi Cai1, Chen Guilan1 and Guohua Liu1*
...ed groups than control. Digestibility of DM, CP, AME, Ca and P digestibility significant (P<0.05) higher in AZO 0.25% and 0.50% dietary treatments. Moreover, jejunal amylase and trypsin activity were improved in AZO 0.25% and 0.50% supplemented groups. CREAT was significantly increased in AZO 0.50% treatment, while IgG was significantly improved in AZO 0.25% treatment. Tibia diameter (TD), tibia breaking strength (TBS) we...

Mohammed Sirajul Islam1,2*, Nurhusien Yimer1*, Wahid Haron1 , Faez Firdaus Abdullah Jesse1, Mark Hiew Wen Han1, Mamat-Hamidi Kamalludin3, Wan-Nor Fitri1, Ubedullah Kaka4, Abdul Quddus1,5 

...ly contributed by the indigenous Kedah-Kelantan (KK) and their crossbred cattle. This study was conducted to compare growth performance and sexual maturity of KK and KK × Brangus bulls. Ten (10) experimental bulls with similar ages were kept under the same paddock and fed with equal quality and quantity Guinea grasses and palm kernel cakes. Body weight was measured at monthly basis by ...

K.M. Injarul Haque, Sharmeen Islam*, Md. Rokibul Islam Khan, Md. Ruhul Amin 

...ME), and organic matter digestibility (OMD) were improved (p<0.05) but dry matter (DM), crude fiber (CF), ether extract (EE), and ash were declined (p<0.05) in all the treatments (T1, T2, and T3) compared to control T0. In the consideration of results among all the treatments, T2 and T3 up to 28 days were desirable for the preparation of silage. To sum up, rumen content can be utilized in rice straw and napier leftovers in silage preparation which provid...

Tintin Rostini1*, Irwan Zakir1, Danang Biyatmoko2 

...consumption, dry matter digestibility, organic matter, crude protein, NDF, ADF, daily body weight gain, feed conversion, and blood metabolism. Furthermore, the data obtained were analyzed using analysis of variance while differences between treatments were further analyzed with Duncan’s test. The results showed that yeast + curcuma (TR3) supplementation had a significant performance by increasing consumption, dry matter dige

I Nyoman Sulabda1*, I Nengah Wandia2, I Ketut Puja3 

...ro white cattle is an indigenous and endangered cattle population in Taro village, Bali and only inhabiting restricted areas in forests of Taro village. Current population are 57 head. To provide basic information concerning allele and locus in BoLA gene, the BoLA microsatellite loci (DRB3 and BM1815) were used to evaluate allel diversity using 18 samples of Taro white cattle. Genetic variability was low, with observed heterozygosity and expected heterozygosi...
Johar Hussian1, Faiz Muhammad2, Shafqat Fatimah Rehmani3, Aqeel Ahmad4, Nazir Ahmad Lone4, Moomal Bughio5, Syed Khurram Freed1 and Shakeel Ahmed Khan4*
...or preparation of IBV antigen by treating with different enzymes for HA reaction. IBV local isolates were characterized by SDS-PAGE and RT-PCR. The indigenous isolate HA antigens were treated with different proteolytic enzymes trypsin, neuraminidase and phospholipase C. The prepared antigen were stored at -86oC and used for HA test. All ant

Neven Waheeb1*, Sherif Marouf2, Essam Nasr3, Shaymaa Abdelmalek2 

...vely detect H. pylori Antigen in human stool and fasces of pet animals, and PCR by using 16srRNA primers through a semi-nested PCR technique.

Keywords | ELISA, PCR, 16srRNA 

...

Grace Chinenye Onyishi, Chinedu Ifeanyi Atama*, Fejiokwu Esther Chioma, Ifeanyi O Aguzie, Godwin I Ngwu and Christopher Didigwu Nwani

...world in general and in Nigeria in particular are fragmentary and not readily available. Given this paucity of information, the present work is aimed at investigating gut parasites of donkeys and horses of Udenu Local Government Area (LGA), Enugu state, Nigeria. Standard procedure of parasite search in vertebrates was used. A total of 23 donkeys and 24 horses were examined for gut helminth parasites. In total, out of 47 equi...

Tran Thi Bich Ngoc1,2, Ninh Thi Huyen1,2, Nguyen Cong Oanh2, Pham Kim Dang2* 

...h performance, nutrient digestibility, and fecal noxious gas concentration in grower-finisher pigs. Twenty-four barrows [(Pietrain × Duroc) × (Landrace × Yorkshire)], with an average initial body weight of 26 ± 0.77 kg (around 75 days old), were equally divided into four dietary groups, 6 replicates each group. The animals were housed individually in metabolism cages. The four experimental diets consisted of a basal diet (CONT), PROB d...

Pham Tan Nha1*, Nguyen Thi Kim Dong2, Le Thu Thuy1 

... growth performance and digestin for growing Tau Vang chicken production. It showed that ginger made quantification of triglycerid and quantification of total cholesterol in chicken blood decrease.

Keywords | Tau Vang chicken, Ginger, Triglyceride, Cholesterol and digestion 

...

Aly M. Ghetas1*, Dalia M. Sedeek1, Hanaa S. Fedawy1, M.A. Bosila1, Hoda M. Mekky1, Kh. M. Elbayoumi1, 3, Mohamed M. Amer2 

...ies); A. flavus (2), A. niger (1) and A. terreus (1). Molecular identification of 5 representative Aspergillus isolates indicated A. fumigatus (2), A. flavus (2), and A. niger (1) were also performed. We recommended further studies to identify the intestinal fungi of chickens in large scale and different ages which help to understand their role.

Keywords | Aspergillus, Chickens, fungi, immunosuppression, molecular id...

Jarmuji Jarmuji1,3, Lili Warly2*, Mardiati Zain2, Khasrad Khasrad2

... fermentation products, digestibility of nutrients, and gases production. Sakura block plus is a modification of commercial sakura block by using earthworm flour as a source of protein as a substitute for rice bran. The treatments were arranged as follow: P0= OPF + concentrate + 10% commercial sakura block, P1 = OPF + concentrate + 6% sakura block plus, P2 = OPF + concentrate + 8% sakura block plus, P3 = OPF+ concentrate + 10 % sakura block plus, P4 = OPF + co...
Indah Prihartini1*, Miftachi Ari2, Manar Fayiz Mousa Atoum3,4, Akhis Soleh Ismail1 and  Listiari Hendraningsih1
...Oryza sativa L.) on digestibility and efficiency of microbial protein synthesis using in vitro residual gas production and the optimal lignolitic probiotic in rice straw. The materials used were rice straw IR 64 cultivar and lignolitic TPG probiotic. The method used was Randomized Complete Block Design (RCBD) with four levels of treatments and three groups. In addition, a further test was conducted using Duncan’s multiple range due to a significant d...

Martha Echioda Ogbole*, James Agbo Ameh, Samuel Mailafia, Olatunde Hamza Olabode and Bridget Jessica Adah

...ity rate (CFR) of LF in Nigeria with a view to establish the disease burden and dynamics, and to guide a sustainable control plan. The LF weekly epidemiological reports were obtained from the Nigeria Center for Disease Control (NCDC) between January 2016 - January 2021. Positive cases were confirmed based on laboratory confirmation of LV- transcription polymerase chain reaction (LASV-RT- PCR). The number of suspected and con...

Shang Zhenda1,2*, Kong Qinghui1,4*, Li Jiakui1,4, Liu Suozhu1,2, Tan Zhankun1,2, Shang Peng1,3 and Wang Honghui1,2

...n important role in the digestion, absorption and health of animals. Studying the intestinal flora of yak has significance to improving its productivity and immunity. In this study, we analysed the bacterial diversity in fresh faeces of wild yak and domestic yak. Results showed that the structure of the bacterial flora in wild yak and domestic yak was significantly different. Firmicutes, Actinobacteria, Bacteroidetes and TM7 were the dominant phyla, and Microc...

Lin Huang1, Ling Mai2, Keyan Zhong1 and Xinjun Chen3*

...ics, signal peptides, antigen epitopes, transmembrane domains, secondary and tertiary structures, multi sequence alignment and molecular evolutionary tree of SeFABP were predicted and analyzed. On this basis, SeFABP was whole-genome synthesized and cloned into prokaryotic expression vector. The recombinant plasmid pET-30a(+)-SeFABP was constructed, then transformed into E. coli BL21 (DE3) and induced by IPTG. The purified SeFABP was obtained by Ni-IDA resins a...

Alexander Tsybulsky1*, Tatyana Tabakaeva1, Anna Klimovich2, Michail Shchelkanov1, Eduard Kostetsky1, Maksim Aliev1, Anton Degtyarenko3 

...ral p27-FeLV-specific antigen in the blood plasma; 5) Expression of ifnα6, ifnα7, ifnβ1, ifnγ and ifnλ1 genes in WBC; 6) Expression of p53 and gadd45g genes in WBC. The concentration of the viral p27-FeLV antigen in the blood plasma was determined by ELISA. The total DNA and total RNA was extracted from the WBC. The cDNA was obtained using an mRNA matrix. Qualitative and quantitative analyses of...

Ahmad-Ur-Rahman Saljoqi1, Sumayya Amin1, Muhammad Salim1*, Taufiq Nawaz2 and Farida Anjum3

...d against Helicoverpa armigera was conducted in tomato crop during 2019. The experiment was carried out in (RCBD) Randomized Complete Block Design. There were four treatments i.e., Cypermethrin, Imidacloprid, Lamda cyhalothirn and control. Each treatment was replicated three times. Lowest mean percent fruits infestation (2.84%), highest yield per treatment (9.56 kg) per plot, highest ascorbic acid content (11.06 mg/100g), fresh weight of treated tomato fruits ...

Chukwudi Njoku1*, Inyang Etim-Inyang2, Prince-Charles Itu1 and Arinze Uzoezie1

...e in Cross River State, Nigeria. A Geographic Information Systems (GIS) Multi-Criteria Evaluation (MCE) technique was applied using secondary data and considering factors such as elevation, land-use, accessibility (roads), river width, population density, distance from anthropogenic activities and climatic variables (temperature and rainfall intensity). The criteria and threshold for siting Tilapia cage culture were adopted from previous studies and processes ...

Arif Mehmood*, Muhammad Naeem and Ata-ul-Mohsin

...rough March-November. Armigeres spp. through January-November, Aedes spp. through April-November and Lutzia spp. were found in the months January-March and June-November. Seasonal distribution pattern is an asset in controlling any epidemic occurred due to mosquito vector. This pattern informs about the active period, favorable temperature and humidity ranges of the mosquitoes, hence the clues for controlling.

...

Javed Khan1, Abdul Majid1, Mohammad Nisar2*, Ali Hazrat2, Nausheen Nazir3, Muhammad Zahoor3, Mohammad Ihsan2, Azhar Hussain Shah1, Muhamad Ajmal Khan4 and Muhammad Yahya1

...f 51.43 to 100% with an Eigenvalue of 0.25. It was concluded from the results that Alnus nitida L. genotypes available in Pakistan have come from a narrow gene pool and such types of variations can be exploited to develop new varieties with desirable traits. 
...

Hung Van Mai1, Tuan Duc Nguyen2, Nang Thu Tran Thi3 and Hung Phuc Nguyen4*

...BM2 and FSBM3) on lipid digestion status, including plasma lipid level, liver and muscle lipid contents, bile acid (BA) concentration, lipase activity, and lipid apparent digestibility coefficient (ADC) in pompano. The FSBM1 was produced by fermentation of defatted soybean meal (SBM) with Bacillus subtilis B3. The FSBM2 and FSBM3 were commercially available products, which were produced by fermentation of SBM with Lactobacil...

Zhang Dong-jie1, He Xin-miao1,2, Wang Wen-tao1,2 and Liu Di1,2*

...tify;">The Min pig is indigenous to China. The genetic background of this breed was previously unclear, limiting the utility of the Min pig. In this study, the whole genomes of ten Min pigs and four Northeast wild boars were sequenced and the analysis yielded 8,988,338 non-redundant SNPs plus 1,231,680 InDels. A phylogenetic tree was constructed and a principal component analysis (PCA) was performed based on previously published SNP data from 66 individual pig...

Wafaa M.A. Ghoneem, Reham R. El-Tanany and Adel E.M. Mahmoud*

...ces among groups in the digestion coefficients (%) of dry matter, crude fiber, ether extract, nitrogen-free extract, and feeding value expressed as digestible crude protein. And there were insignificant (P<0.05) differences between G1 and G2 in the digestibility (%) of OM, CP, ADF, and TDN values. While the highest nutrients digestibility and nutritiv...

Sozan A.A. Ismaeil1*, Hassan Emam2 

...laughtered young homing pigeons and Ibis. The samples were fixed and processed for the histological and histochemical examination of the liver. Pigeons were collected from the squab breeder while Ibis were collected from streets at Shariq province. The morphological findings showed that the liver of two species of birds is located in a hepato-peritoneal cavity, and each is composed of two undivided lobes, a large right lobe ...

Junaid Ahmad1*, Shazma Anwar1, Anwar Ali Shad2, Sher Shah Souri1, Bibi Amina3, Wajia Noor4, Abidullah1 and Muhammad Adil1

...ein contents and better digestibility than any other pulse crop. A field study was carried out at Agronomy Research Farm, The University of Agriculture, Peshawar in summer season 2018 with objectives to find out impact of molybdenum (0, 0.5, 1.5 and 2.5 kg ha-1) and phosphorus (0, 30, 60 and 90 kg ha-1) application on performance of mungbean. The trial was presented in randomized complete block design having three replicates. For molybdenum and phosphorus sour...

Muhammad Tariq Mahmood1*, Muhammad Akhtar2, Kaiser Latif Cheema2, Abdul Ghaffar3, Imtiaz Ali4, Muhammad Jahanzaib Khalid2 and Zeshan Ali5

...1 and 2 extracted >1 Eigen values explaining that these components have major contribution in genetic variability. Cluster analysis distributed the genotypes into four distinguished clusters. Agglomerative dendrogram of genotypes was constructed by Ward’s method. On the basis of Euclidean distance it was observed that members of cluster 3 (G.P-110, G.P-111, G.P-115, G.P-116, G.P-121, G.P-125, G.P-138, G.P-139, G.P-150, G.P-151, G.P-164, G.P-165) and c...

Omer Eyuboglu

...transportation of non-indigenous species (NIS) and their permanent habitation of secondary regions pose a critical threat that adversely affects the marine environment and marine based economic activities and applications. The transportation of NIS causes a decline in fish industries, spoilage of culture fishing stocks, rising production costs, adverse effects on human health and changes in biological diversity. The most prominent alien species in Turkish Blac...

Shakila Mumtaz1, Khalid Javed1, Muhammad Dawood1*, Muhammad Imran2, Asad Ali1 and Nazia Ramzan1

Farheen Aslam*, Hira Lodhi, Rasheeda Bashir, Faiza Saleem and Shagufta Naz

...2.5% E. coli and 6.25% shigella sp. Nucleotide sequencing of PCR product of Klebsella sp, Enterobacter cloacea and Citrobacter freudii showed 100% sequence homology. It is concluded that there is high prevalence of blaNDM-1 among carbapenem resistant enterobacteriaceae isolated from patient suffering from different diseases at local tertiary care hospital of Lahore.

...
Soliman Mohammed Soliman
...iae or Yucca schidigera) on in-vitro total gas and methane production, in-vivo rumen fermentation parameters, and milk production in dairy cows. Twenty Friesian cows were randomly allocated to four groups (five cows /group). All cows were fed TMR (total mixed rations). The first group was fed TMR without supplementation, groups from 2 to 4 were fed TMR supplemented with one of the feed additives S. cerevisiae, yucca, or gre...

Fatma Desouki Mohammed Abdallah

...words | Carcass traits, Eigen-values, Japanese quail, Principal component analysis, Scree plot
...

Hassan Ali1, Abu Bakar Muhammad Raza1, Muhammad Zeeshan Majeed1* and Muhammad Imran Hamid2

...re recommended to the indigenous farmers as biorational options for the management of T. granarium and other stored grain insect pests.

...

Asmaa Sh. Fayed1*, Safaa M. Abo El-Soud2 

...l meat chunks during refrigerated storage. After coating meat samples, we analyzed their microbial, chemical, and sensory properties after 0, 3, 5, 7, 9, and 11 days of chilled storage. GA/ zein wax sheets significantly reduced (p < 0.05) the abundance levels of total bacteria, and psychrotrophic bacteria, as well as pH, thiobarbituric acid (TBA), and total volatile base nitrogen (TVB-N), especially for 4% GA/ zein wax film. The coatings resulted in the pro...

Halidou Maiga Nafissatou1*, Abdou Moussa Mahaman Maâouia2, Moumouni Issa1, Marichatou Hamani1 

...font>r. 

...

Sana Shakoor, Tahir Rehman Samiullah, Naila Shahid, Abdul Qayyum Rao*, Aneela Yasmeen, Sana Tahir, Ayesha Latif, Saira Azam, Ahmad Ali Shahid and Tayyab Husnain

...en as the immunogenic antigen because it is highly conserved among H9N2 strain of avian influenza virus. Moreover, immunogenic regions within HA gene may contain potential immunogenic epitopes. The specificity of HA protein produced from E. coli was confirmed through an antibody-antigen reaction on the nitrocellulose membrane. The appearance of 67 KDa protein on the nitrocellulose membrane confirmed its specificity. The intr...

Nzube Thaddeus Egboka1*, Olajire Fagbola2, Ugochukwu Nnamdi Nkwopara1, Nnaemeka Henry Okoli1, Akaninyene Isaiah Afangide1 and Tochukwu Victor Nwosu3

...an and Ikwuano areas of Nigeria at 0-15, 15-30 and 30-45 cm depths and analyzed in the Laboratory. Spore densities of AMF varied significantly (P > 0.05) between the fallow and cultivated (cassava and pineapple) land use types in both locations. Across the soil depths, however, AMF spore density decreased significantly with depth in Ibadan, with mean values of 54±6, 45±3 and 39±5 spores 100 g-1 soil at the 0–15, 15–30 and 30...

Aymen Mabrouk*

...ient of volatile oil of Nigella sativa seeds, against the deterioration of blood indices by lead (Pb) has never been studied. Therefore, the present study was carried out to evaluate the possible beneficial effect of TQ against Pb-induced hematological changes. Adult male Wistar rats were treated with Pb (2000 ppm of Pb acetate in drinking water) and/or TQ (5 mg/kg/day, per os) for five weeks. Results obtained clearly showed that Pb intoxication significantly ...

Husmaini1*, Riza Andesca Putra1, Indri Juliyarsi1, Tevina Edwin1, Linda Suhartati1, Aditya Alqamal Alianta1, Harmaini2 

... in order to maintain indigenous chicken Indonesian especially in West Sumatera.

Keywords | Kokok Balenggek Chicken, Population Structure, Management, in-situ area, West Sumatera 

...

Taiwo Oladoye Akande*, Dayo Johnson Ogunyemi, Priscilla Funmilola Okunlola, Emmanuel Owolabi, Odetayo Olakanmi 

...ric to moringa. Protein digestibility increased by 5% and 3% in birds fed garlic and the blend respectively while about 4% increase in fat digestibility was observed in birds with PFAs. The PFAs exhibited varied positive influence (P<0.05) on carcass yield, but no difference (P>0.05) was observed in the organ weights of the chickens. The fat deposition was substantially reduced (P<0.05) in birds with PFAs. The blood...

A. Samy1*, H.M.A. Hassan1, Fatma T.F. Abd-El Ghany2, Shama H. Morsy2 

...oisture and fat levels. Digestibility coefficients of CP, CF, and EE, as well as the nutritive values of TDN, DCP, and DE, were considerably improved (P<0.05), although DM, OM, and NFE digestibility was unaffected in all treatments. Considerably, Addition of curcumin or garlic extract boosted final weight, weight gain, and feed efficiency compared to the control group. Dietary treatments were significantly (P<0.05) rai...

Ayu Cusiayuni1, Risan Khaerani Nurfatahillah1, Rakhmad Perkasa Harahap2, Komang Gede Wiryawan3, Dwierra Evvyernie3, Anuraga Jayanegara3* 

...methane production, and digestibility. The data obtained were analyzed by using the mixed-model methodology. Results showed that the addition of lauric acid reduced methane production (P < 0.001). This decrease was followed by a tendency of methanogen population reduction (P < 0.10) and bacteria (P < 0.10) in the rumen. The addition of lauric acid did not alter total volatile fatty acids (VFA), acetate, propionate and butyrate, but tended to decrease ...

Honnakerappa S. Ballari1 and Shashikant S. Udikeri2*

...gous pest Helicoverpa armigera is important in any agroecosystem. The resistance to classical insecticides viz., synthetic pyrethroids, organophosphates and carbamates was global issue in early nineties. Hence many new generation pesticides are in use widely. To know the status of different insecticides belonging pyrethroid, ogranophaopsahte and carbamate molecules monitoring was exercised using populations of H. armigera co...

Nuraini Nuraini*, Mirzah Mirzah, Yuliaty Shafan Nur, Harnentis Harnentis 

...ity, crude fiber, fiber digestibility, crude protein, and nitrogen retention. The results of phase 1 showed that the types of fungi had a very significant effect (P <0.01) on the nutrient quality of fermented Azolla microphylla. Fermentation of Azolla microphylla with Lentinus edodes and Pleurotus ostreatus gave the best products. Phase 2. Application Fermented Azolla microphylla (AMF) in the diet on broiler performance. Fermentation with Lentinus edodes h...

Muhammad Ammar Dilawar1,2, Hong Seok Mun1, Myeong Gil Jeong1, Eun Ju Yang3, Hyeoung Seog Park4 and Chul Ju Yang1,2,*

...t and thigh meat yield, digestive organ weights and meat quality characteristics in broilers. A total of 210 broilers were divided into the following treatment groups: (i) control, (ii) T1 (0.05% 4 MA: 1 GT + 0.01% LM), (iii) T2 (0.05% 4 MA: 1 GT + 0.05% LM), (iv) T3 (0.05% 4 MA: 1 GT + 0.1% LM), (v) T4 (0.1% 4 MA: 1 GT + 0.01% LM), (vi) T5 (0.1% 4 MA: 1 GT + 0.05% LM) and (vii) T6 (0.1% 4 MA: 1 GT + 0.1% LM). Significantly improved weight gain (P<0.05) and...

Laiba Ajmal1, Sidra Ajmal2, Maleeha Ajmal3, Gul Nawaz3, Rabail Hassan Toor4, Hooria Younas1, Tassaduq Hussain Sheikh5 and Raazia Tasadduq1*

...ng of human leukocyte antigen (HLA) system at the maternal fetus interface plays a pivotal role in acceptance of the fetus. Alterations in the modulated activity of HLA system influence the fetal tolerance. Therefore, this review will provide an insight on how the working of HLA at the maternal fetal interface contributes towards recurrent pregnancy losses (RPL). Antigen hiding is a phenomenon that contributes towards immune...

Laiba Shafique1*, Mahroze Fatima2, Syed Zakir Hussain Shah3, Muhammad Afzal4, Huma Naz5, Saif ur Rehman1 and Qingyou Liu1*

...to determine intestinal digestive enzymes activity and bone mineralization of Ctenopharyngodon idella. Four type food were prepared by using formic acid (%) and vitamin D3 (IU/Kg) in experiment viz, FD1, FD2, FD3 and FD4, respectively. Fish fed with formic acid showed considerable increase in calcium, magnesium potassium, however vitamin D3considerably increase iron and manganese contents (µg/g) in the bones of C. idella. Amylase and protease activity wa...

Aml M. Ragab1*, Maha R. Basyoni1, Enas A.I. Khoris2, Nadia A. Abd Elghany3 

...ytK), and (ces) enterotoxigenic genes. B. cereus isolates were analyzed for antibiotic susceptibility. A lab trial was conducted for two weeks using 60 Tilapia fish were divided into three equal groups, (1): kept as control negative, (2): infected intraperitoneally with (0.1ml) 8×107 (CFU/ ml/ fish) B. cereus on 1st day, (3) infected I/P intraperitoneally with (0.1ml) 8×107 (CFU/ml/fish) B. cereus on the first day and treated with erythromycin (sen...

Uzochukwu Ephraim Emeto, Chukwuemeka Calistus Okolo* and Nwakaego Ernestina Nweze

...s. In countries such as Nigeria, where control of these endoparasites relies on periodic use of anthelminthics, epidemiological evaluations are necessary for deploying meaningful intervention strategies. Such investigations are lacking in the region; hence, the occurrence, risk-factors and nature of cestodiasis and nematodiasis of horses at Obollo-Afor southeastern Nigeria were investigated. Horses billed for slaughter were ...

Bridget Adah1*, Clara Kwanashie2, Haruna Kazeem2 and Samuel Mailafia1

...irosis in Kaduna State, Nigeria predicated this study. The research was conducted to isolate Leptospira species from pigs. The organisms were isolated using Ellinghausen-McCullough-Johnson-Harris (EMJH) enrichment and basal medium, and identified using dark field microscopy. A total of two hundred (200) blood samples and two hundred (200) urine samples each were collected from pigs in Kaduna state, Nigeria. A total of 9 (4.5...

Mirtneh Akalu1,2*, Takele Abayneh2, Esayas Gelaye2, Behailu Tefera2, Teferi Degefa2, Vemulapati Bhadra Murthy1 

...wide range of surface antigens. This study was designed to evaluate the immunogenic potential of M. haemolytica serotype A:1 OMVs (MH-OMVs). Fifteen calves were divided randomly into three groups of five calves and immunized with single-dose and booster-dose of 0.15 mg/ml vesicle preparation. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) assay of the partially purified MH-OMVs revealed approximately ten protein bands ranging from 25 to 1...

Nkiru E. Ekechukwu, Felicia N. Ekeh, Chinenye M. Ohanu, Hope C. Ezinwa and Ifeanyi Oscar N. Aguzie*

Wafaa M.A. Ghoneem*, Muhammad M. Shaklouf, Ali M. Ali and Mohammed H. Bakr

...lbac®) on nutrients digestibility, milk yield and composition, blood biochemistry and immune system response of the animals. Culbac® was added at 0, 10 and 20g/h/d in G1 (control), G2 and G3, respectively. Digestion coefficients of the most nutrients and nutritive value did not significantly (P<0.05) differ between G2 and G1. However, G3 recorded lower values. WBCs, RBCs, Ht, Hb, lymphocytes, monocytes, basophils,...

Lopamudra Samantaray*, Yashaswi Nayak 

...nce, appetite, and food digestion. We aimed to assess the effects of dietary phytobiotic supplements containing black pepper, fennel, and turmeric EOs on the performance of Broiler chickens. The study used 280; 70-day-old chicks. The birds were divided into seven experimental groups; one control and six were fed on phytobiotics EOs at rates of 1% and 0.5% and monitored for 8 weeks. The results revealed that the inclusion of 0.5% black pepper and 0.5% fennel co...

Mujahid Tanvir1, Muhammad Asam Riaz1*, Muhammad Zeeshan Majeed1, Mazhar Iqbal Zafar2, Muhammad Tariq3 and Muhammad Bilal Tayyab1

...hexane extracts of 40 indigenous plant species collected from Soon Valley and surrounding salt range of Pakistan bioassayed against C. quinquefasciatus larvae, eighteen botanicals exhibited more than 50% larval mortality in 48 h exposure. The most effective botanical extracts were Maerua arenaria Forsk, Nerium indicum Mill., Withania coagulans Dunal, Suaeda fruticosa (L.) Delile, Olea ferruginea Wall., Adiantum capillus-veneris L. and Dicliptera bupleuroides N...

Nausheen Irshad1, Maria Akhter1, Tariq Mahmood2*, Faraz Akrim3, Muhammad Rafique Khan1 and Muhammad Sajid Nadeem4

...birds (domestic hen and pigeon), while among plant food it consumed seeds and twigs of wild mulberry, Japanese fruit, wild fig and cucumber. The study concludes that Altai weasel in Bunjosa Game Reserve occurs at an elevation range between 1773 m to 1875 m, and consumes most frequently insects, followed by small mammals, birds and reptiles, along with seeds and twigs of some wild plant species.

...
Nguyen Binh Truong1,2,3*, Nguyen Van Thu3
...tter and organic matter digestibility were gradually reduced (P<0.05), however, there was no significant differences between the NDF47 và NDF55 treatment (P>0.05). Before and 3 h after feeding the rumen pH values, N-NH3 and total volatile fatty acids concentration of cattle were no differences and good for the rumen activities. In this research content, the NDF55 treatment revealed an expectation for the applied studies. Results of the second expe...
Tri Eko Susilorini1*, Ahmad Furqon2, Wike Andre Septian1, Desinta Wulandari3, Suyadi Suyadi3
Paulus Klau Tahuk*, Oktovianus R. Nahak T.B., Gerson F. Bira, Adrianus Manek, Donatus Manek, Tobias Y. Purnama, Emanuel Bubun, Bergias Subay
...rthermore, the nutrient digestibility was relatively the same among the treatments, while the daily body weight gain (ADG) of T2 was higher (P<0.05) than T1 and T3. Additionally, the conversion and efficiency on T2 were higher than T3, but relatively the same as T1. It is concluded that the application of complete feed with protein source from Gliricidia sepium with crude protein and TDN of 13 and 72% respectively could improve the fattened Bali cattle perf...

Barirah Rehman Talpur1, Zaheer Ahmed Nizamani1*, Imdad Hussain Leghari2, Mansoor Tariq1, Aisha Rehman3, Shahnawaz Kumbhar1 

...s along with lesions in digestive system of broilers.

Keywords | Fluorosis, Rabbits, Broilers, Liver, Kidney 

...
Manatsanun Nopparatmaitree1*, Pornpan Saenphoom1, Sittichai Bunlue1, Silchai Washiraomornlert1, Warangkana Kitpipit2,3,4, Soranot Chotnipat1
...prove gut microbiology, digestibility, and performance of broilers. This experiment was carried out to investigate the effects of synbiotic from trimmed asparagus by-products (TABP) combined with probiotic supplementation in broiler diets on the apparent nutrient digestibility, cecal microbiota, small intestinal morphology, and performance. Three hundred twenty-one days old, Ross 308® chicks; were raised under ambient te...

Mohamed El-Henfnawy*, Essam A. El-Gendy, Ahamed M. El-Kaiaty, Mostafa Helal 

...nbsp;

...

Hany M.R. Elsherif1, Ahmed Orabi2, Hussein M.A. Hassan3, Ahmed Samy3*

..., immunological status, digestive parameters, and microbiota.
 
Keywords | Blood parameters, Broiler chicks, Gut health, Immune status, Organic acids salts, Productive performance
...

Abd El-Moniem Ali S. Mahgoub, Ahmed Mohamed Abd El-Hafeez, Mahmoud Yassin Mohamed*, Al-Moataz Bellah Mahfouz Shaarawy, Mohamed Ibrahim Nassar

...ver hay on feed intake, digestibility, growth performance, and semen quality. Animals were randomly divided into four groups and fed four different rations. Ration 1 (R1) was the control (60% concentrate feed mixture + 40% clover hay). The remaining rations were supplemented with sesban hay by 10% (R2), 20% (R3), and 30% (R4) as a replacement from the clover hay ratio in the control ration. Results showed that supplementation of sesban hay increased the nutrit...

Sepri Reski*, Maria Endo Mahata, Ridho Kurniawan Rusli

...inaria murayana) with Indigenous Micro-Organism (IMO’s) from fruit waste as starter on performance, carcass yield and giblets percentage. A total of 100 one- d- old Arbor Acres CP 707 broilers was raised for 4 weeks of age. The birds were distributed into 5 treatments with 4 replicates in a completely randomized design. The fermented seaweed was supplemented at the level of 0, 5, 10, 15 and 20 % in the diets. The observed variables were live weight, body...

Mimi Syazwani Jaapar1, Eric Lim Teik Chung1,2*, Nazri Nayan1, Mamat Hamidi Kamalludin1,2, Mookiah Saminathan3, Kalai Vaani Muniandy2, Muhammad Hazziq Mohd Hamdan1, Shokri Jusoh1, Faez Firdaus Abdullah Jesse2,4 

...in vitro organic matter digestibility, ammonia nitrogen, and volatile fatty acids (VFA). All analyses were conducted according to established methods and data collected were subjected to one-way analysis of variance (ANOVA) and Duncan’s Multiple Range Test to determine the level of significance among treatments. The net gas production and gas production kinetics demonstrated notable changes (P < 0.05) among treatments. Besides, only the acetic acid an...

Avijite Sarker, Sharmeen Islam, S.M. Ariful Islam, Md. Rokibul Islam Khan*, Md Mukhlesur Rahman 

...in vitro organic matter digestibility (OMD) and other nutritional properties, at different intervals (0, 30, 45, and 60 days). The organoleptic and physical characteristics (smell, color, and hardness) of feeds were improved in treatments with market fish wastes up to 60 days but after 45 days, in T3, fungi and some bad smells were obtained. The pH, dry matter (DM), crude fiber (CF) were reduced and the crude protein (CP), ether extract (EE), ash, ME, OMD were...

Ghulam Abbas1*, Muhammad Arshad1, Muhammad Saeed2*; Safdar Imran3, Ashgar Ali Kamboh4, Duraid KA Al-Taey5, Muhammad Asad Aslam1, Muhammad Saeed Imran6, Muhammad Ashraf3, Muhammad Asif7, Abdul Jabbar Tanveer8, Razia Abdul Majid Qureshi9, Maria Arshad1, Hussain Ahmed Khan Niazi1, Muhammad Tariq10, Sikandar Abbas1 

...o enhance the uptake of digested proteins and important minerals. The advantages of using OA as feed additives greatly outweigh their disadvantages like decreased palatability. Organic acids can increase egg productivity and enhance the egg quality in layers. In broiler, use of OA is associated with improved weight of birds and feed conversion ratio. Dietary OA showed 1.85-8.48% increase in the FCR of chicken. Lactic acid fed 0.3 g/kg diet reduced Escherichia ...

Adimabua Mike Moemeka1, Ufuoma Godstime Sorhue1*, Sylvanus Ikenna Omeje2, Lawrence Bratte2, Raphael Onainor1, Okpara Oghenesuvwe2 

... in the maize. Nutrient digestibility was significantly different (P<0.05) among treatments. The average total weight gain ranged from 13.22kg in T2 to 17.18kg in T1 followed closely by 16.44kg in T3. There were significant differences (P<0.05) among the treatments for all the performance characteristics studied, except average daily weight gain and feed conversion rate. Average daily weight gain was 0.24kg in T1 and 0.17kg in T2, while T3 had the highes...

Mehwish Saleem Khan* and Sumama Farooq

...s of brain, eyes, ears, digestive tract, respiratory tract and urinary tract. It has become difficult to treat these infections as these microbes have become resistant to variety of antibiotics. Due to multidrug resistance only few choices are left to treat the bacterial infections.

...

Akbar Hayat1*, Marayam Nasir2, Ehsan-Ul-Haque1, Muhammad Asim1, Sohaib Afzaal1, Shagufta Naz3, Muhammad Nawaz1, Abid Mahmood4, Faheem Khadija1, Fiaz Hussain1 and Tariq Mehmood5

...four overseas and two indigenous rootstocks in Pakistan. Experiments were conducted at experimental station of Citrus Research Institute, Sargodha from 2016 to 2020. Results revealed over all good adaptability, while no significant difference in juice contents was observed among all the tested cultivars. Physical quality of fruit (weight, firmness, size etc.,), and TSS were significantly affected by varying rootstocks. Cox mandarin was found as best cultivar a...

Rizwana Abdul Ghaffar* and Kamil Nadeem

...="text-align: justify;">Pigeons are the warm-blooded vertebrates that belong to Class Aves. They are used for many purposes like for rearing and food are the important aspects. They become parasitized by many of the parasites through their feeding and feeding habitat or by some infected birds that live in association with them. The majority of the nematode parasites that inhabit the body of the pigeon reside in the intestine...
Yasmin M.M. Mahmoud
...ts, rations on nutrient digestibility, milk production, some physiological properties of blood and hematological profile. Animals were randomly divided individually into three comparable groups (eight does/group) in a comparative feeding trial. First group was received concentrate feed mixture (CFM) and BH (60:40 %) as control diet (T0). Tested groups T1 and T2 were given rations containing (BB) 10 % or 20 % as replacement of (BH) in the control diet, respecti...

Teguh Wahyono1,2*, Wahidin Teguh Sasongko3, Wijaya Murti Indriatama4, Setiawan Martono5, Slamet Widodo3, Widhi Kurniawan6, Muhamad Nasir Rofiq7

...composition or in vitro digestibility. The effect of different variety on the nutrient value of sorghum silage was more pronounced than the wilting variable.
 
Keywords | In vitro digestibility, Nutritive value, Silage, Sorghum, Variety
...

Oluwaseun Adetarami1*, Oluwatoyin Olagunju2, Babatunde Adebayo Oyebamiji1, Adegboyega Abel Odeyemi3 and Sina Basil Johnson4

...Ondo and Osun States of Nigeria. Multistage sampling procedure was used to select (160) respondents. The primary data were collected through structured questionnaire. For the data analysis, statistical tools such as budgeting technique and logitistic regression were used. Results of the budgeting technique showed that each farmer of the FBS approach had a mean profit of ₦ 81,134.65 with a cost benefit ratio of 1.09. The results of logistic regression reveale...

Mohamed E. El-Speiy1, Tarek A. Sadak1, Mohamed A. Abd-Elaal1, Ayman M. Khalifah2, Amr S. Morsy2*

...n enhanced all apparent digestibility coefficient parameters compared to other treatments. In addition, carcass traits were improved by AG2, Zn, AG1Zn, and AG2Zn treatments. Regarding economic efficiency, each AG2 or AG1Zn and AG2Zn represented the most promising treatments. Results referred that an individual dose of (AG2) or its combination (AG1Zn and AG2Zn) maximizes the growth performance and economic efficiency of growing rabbits.
 
...

Yasmin M. M. Mahmoud* 

...their diets on nutrient digestibility, growth performance, some blood parameters and hematological profile. Animals were chosen with an average body weight 15.40±1.99kg and divided randomly into three similar groups (8 kids/each). The 1st group (G0) was fed the control diet formulated from concentrate feed mixture and berseem hay with rate of 60 and 40%, respectively. Kids in the 2nd (G1) and 3rd (G2) groups were fed the control ration supplemented with...
Basel A. Abokhadra, Samah M. Mosad, Sahar Abd El Rahman*
...BoHV-5) have the same antigenic and genetic relatedness. BoHV-1 is one of the viruses that incriminated in respiratory disease; affects the genital tract and causes significant economic losses to domestic cattle in Egypt. Although BoHV-5 mainly causes encephalitis in calves, the virus is excreted in nasal discharge under stress factors, so herpesvirus latency reactivation cycle is the hallmark of the disease. In the present study, molecular screening of 60 nas...

Atta Ullah*, Nasrullah Khan, Ataur Rahman and Rafi Ullah 

...itants from different indigenous angiosperms and their products in Sheikh Buddin National Park. Information from 250 respondents (240 male, 10 females) was gathered randomly about plants’ medicinal and folk uses in the park. One hundred and sixteen plants species belonging to 99 genera and 50 families were recorded and identified with the help of available literature. Of these, monocots were represented by 17 species under 15 genera and 5 families, while...

Imtiaz Ahmed*, Imran Khan and Zia Ud Din

...h“in-vitro starch digestibility”. The ash, fiber and protein content increased significantly while the carbohydrate content had significantly lower values (p, for all trends < 0.05) as the ratio of incorporation increased when compared with the control sample (data not shown here). The CWB also showed significantly (P<0.05) higher phenolic and total flavonoids content. Furthermore,CWB showed significantly (P<0.05) higher antioxidant activi...

Ziaul Islam1*, Asad Sultan1, Sar Zamin Khan1 and Sher Bahader Khan2

...ble energy and nutrient digestibility of broilers. A total of six hundred, day-old healthy broiler chicks (Cobb 500) were procured from a local hatchery and were randomly allocated to five different dietary treatments. A basal diet formulated with corn-soybean meal (CON; without any additives) served as control diet and fed to birds in control group. Birds in other four groups were given same basal diet mixed either with zinc bacitracin (150 mg/kg diet; ZB-150...
Baila Ahmad1, Muhammad Ammar Khan1*, Zulfiqar Ahmad1, Rana Muhammad Bilal2 and Asghar Ali Kamboh3
...reatment for 15 min, refrigeration for 3 d, and pressure treatment of 196 N for 15 min) was evaluated. The significantly (p<0.05) lowest EM, but highest (p<0.05) WHC of T3 samples depicted raw meat processing quality improvement. Moreover, dietary incorporation of 40 ppm Zn resulted in a significant (p<0.05) decline (1.88%) in DL as compared to the control that illustrated remarkable raw meat yield improvement. Similarly, the reduction (p<0.05) of ...

Abdul Ghaffar*, Niaz Hussain, Muhammad Nadeem, Khalid Hussain, Muhammad Aslam, Mudassar Khaliq, Muhammad Irshad, Zubeda Parveen and Muhammad Younas

...expressed more than one Eigenvalue which collectively contributed 80.12% towards the genetic variation. High positive loadings were expressed by the secondary branches per plant and pods per plant in all components. Path analysis revealed that secondary branches/plants expressed the highest direct positive effect (0.872) grain yield followed by the pods per plant (0.623). Analysis of correlation coefficient also confirmed the highest significant contribution o...

Bibi Hajira*, Imran Khan and Zia-ud-Din

...ause of its high starch digestibility. Previous studies suggested that the addition of other grains, legumes and medicinal herbs to bread might reduce its starch digestibility. However, the incorporation of these non-wheat materials into bread is challenging from consumer acceptability perspective. Therefore, the current study investigated the effect of incorporating red sorghum and barley flours in white bread on in-vitro s...

Ochieng Onunga George*, Owuor Ndonga Millicent Florence, Mario Kollenberg  

...mal trimmings, horns, undigested ingesta (paunch contents), bones, and hairs. In contrast, liquid parts consist of blood, dissolved solids, urine, gut contents, and wastewater from slaughter operations and floor cleaning. It has been reported that Abattoir wastes can have negative impacts on the areas surrounding them by causing pollution and public health concerns. Therefore, this research was initiated with the main objective being to evaluate the Parasitolo...

Taufiq Bachtiar1, Muftia Hanani2, Anisiyah2, Winda Puspitasari2, Wahidin Teguh Sasongko3, Teguh Wahyono4*

...t profiles and in vitro digestibility of soybeans. Thirty soybean genotypes were evaluated for nutrient composition, fiber content, and in vitro digestibility after cultivation in pH 4.0 and 5.4 soils. The experiment was arranged factorially in a completely randomized block design. The experimental factors were soybean genotype and soil pH. Results showed significant (p < 0.001) differences in fiber content, non-fiber car...
Eman M. Aboelela1, Mohamed A. Sobieh2, Eman M. Abouelhassan3, Doaa S. Farid4, Essam S. Soliman2*
...is flea, Heterodoxus spiniger lice, and Sarcoptic scabiei mites in dogs, Otodectes cynotis ear mites, and Ctenocephalides felis flea in cats. Prevalence of parasitic infestations in dogs and cats showed strong associations with the breed, sex, age, coat color, housing system and pattern, type of food, and type of infesting external parasites concerning seasonal variations.
 
Keywords | Cross-sectional, Dogs, External parasites, Pre...

Muhammad Jamil1*, Kamran Javed1 and Imran Akhtar2

...), first 5 PC indicated Eigen value >1 and contributed 75.826% towards cumulative variability. Yield related traits plus lint quality attributes depicted positive loading behavior, while disease index (-0.215) and plant population (-0.054) showed negative loading attitude in PC-1. Similarly, boll counts plant-1 (0.778) and fiber length (0.480) indicated strong positive loading trend, whereas fiber fineness (-0.742) and ginning out turn (-0.709) showed negat...

Liyun Chang1, Aiju Liu2, Jianshuang Zhang3, Yingbin Chen1 and Zhiyong Liu4*

...hen used as a coating antigen to establish an indirect ELISA method for detecting the T. gondii antibody in pet cats, whose reaction conditions were optimized. The SAG2 gene was successfully cloned into a pET-21a (+) prokaryotic expression vector, and the recombinant plasmid pET-21a-SAG2 was obtained. The size of the recombinant protein was approximately 19 kDa, and it was expressed mainly in the form of an inclusion body. The optimal reaction conditions were ...
Fei Wang1, Xiaofen Hu1, Feng Wen2, Xiaoen Tang3, Shanshan Yang1, Shengwei Zhong1, Zuohong Zhou1, Xu Yuan1 and Yong Li1,*
...e intestinal peptide in pigeon, the present study was aim to further explore the correlation between its molecular bioinformatics characteristics and biological function. After the desired RNA was extracted from the intestine of Wahui pigeons, the complete CDS of vasoactive intestinal peptide was obtained by PCR amplification and sequencing, in addition, a variety of bioinformatics analysis was performed to evaluate its stru...

Ehab A. Fouad1*, Khaled A. Abd El-Razik2 , Eman H. Abdel-Rahman3

...at known as the crude antigen. It entered to affinity column chromatography [Cyanogen Bromide-Sepharose 4B (CNBr-Sepharose 4B)] (Sigma Chemical Co.), obtain the fraction. In brief, IgG of cows S. aureus positive sera were purified by precipitation with 50% ammonium sulphate followed by purification with Protein A Sepharose gel. The separated IgG was dialyzed for 3 days against a 0.1 M NaHCO3 buffer pH 8.3 with 0.5 M sodium chloride and 0.02 percent NaN3 before...

Jie Zhang, Na Li, Tingting Guo, Dandan Hu, Xiaofeng XU and Lili Zhang*

... Tan sheep, a Chinese indigenous breed, using 16S rDNA high-throughput analysis. The rumen fluid was collected from 9-month-old Tan sheep that were either house-fed or grazed. The results showed that the diversity of rumen flora in the grazing group was significantly higher than that in the house-raised group (P < 0.01). In the grazing group, the abundance of 96 genera was significantly higher than that in the house-raised group (P < 0.05), whereas in th...

Mahmoud S. Sirag1*, Effat L. El Sayed2, Mahmoud M. Hussein3, Khalid A. El-Nesr1, Mahmoud B. El-Begawey1 

... distribution of BVDV antigen in the genital organs of persistently infected and acutely infected cows, this study was carried out on genital organs of thirty cows submitted to slaughter houses suffering from reproductive disorders. Pathologically, three mummified fetuses had been extracted from the uterus of examined cows (3/30). The disrupted growing and mature follicles were a distinctive microscopic feature in infected animals while such changes were absen...
Zahra Naz1,2, Fouzia Ismat1, Muhammad Saleem2, Mazhar Iqbal1, Aamir Shehzad1 and Moazur Rahman1,2*
... promising diagnostic antigen for reliable detection of NDV infection in chickens. In the present study, we have devised a strategy for extraction and solubilization of the NDV M protein from Escherichia coli in a single step using a non-ionic detergent, lauryl-dimethylamine oxide (LDAO), enabling the purification of the detergent-solubilized M protein in a soluble form through affinity chromatography without compromising the structural integrity of the protei...

Zaidi Zona, Zulfiqar Ali*, Rida Ahmad and Irfan Zainab

... the water quality. The eigenvalues obtained after PCA from zone 1 to zone 5 were 5.40, 2.40, 1.90, 0.28 and <0.28, respectively. The higher eigenvalue of zone 1 suggests large dispersion of data in this region. Furthermore, the findings in the study indicate that warm water, higher conductivity, DO, high nitrate concentration, and high salinity manifested to be the best habitat conditions downstream of Thakot area. To co...

Muhammad Rizwan, Abu Bakar Muhammad Raza*, Muhammad Zeeshan Majeed and Muhammad Arshad

...re recommended to the indigenous citrus growers combating mealybug infestations on citrus.

...

Mutassim Abdelrahman1, Ibrahim Alhidary1, Majdi Bahaddi1,2, Mohsen Alobre1,3, Riyadh Aljumaah1 and Rifat Ullah Khan4, *

...ial lasted for 56 days. Digestibility trial was conducted at mid of the feeding trial using four lambs from each trial. At end of the trial, four lambs from each treatment were slaughtered and rumen fluid, liver, kidney, and meat were collected for mineral analysis by inductively coupled plasma optical emission spectrometry. Results indicate that there is no significant difference (P >0.05) between the concentration of all the elements in all lambs’ t...

Febriza Dwiranti1, Nur Fadhilah2, Ursula Paulawati Maker2, Priyo Sambodo3* 

Desy Cahya Widianingrum1*, Himmatul Khasanah1, Melinda Erdya Krismaputri1, Susan Barbara Patricia Sembiring Meliala2 

...cteristics and in vitro digestibility. The data obtained were analyzed using Analysis of Variance (ANOVA) and further tested with an Honestly Significant Difference (Hsd). The highest significant difference in physical quality of wafer (P<0.05) were found among treatments. The P4 group had the highest density (0.230 ± 0.16 g/cm3), while the P1 group had the highest absorption (77.60%). The P0 group had the highest thickness swelling (12.95 ± 0...

Haitham Al Masruri1, Rashid Al Zeidi2, Aiman Al Mufarji3, Abd El-Nasser Ahmed Mohammed3, Ahmed Al-Madani4, Al-Hassan Mohammed5* 

...ts extracts on nutrient digestibility, rumen fermentation and enzyme activities, growth and reproductive performances, antioxidants, and milk production and composition have been well documented. M. oleifera contains valuable chemical constituents such as proteins, essential fatty acids, vitamins, and beta-carotene. The M. oleifera composition varies depending on nutrient availability for production, species, and lifespan of trees. Extracts of leaves and seeds...

Ghulam Qadir1, Khalil Ahmed1*, Muhammad Ashfaq Anjum1, Quais Muhammad Affan2, Muhammad Zaighum Mushtaq3, Muhammad Amjad Qureshi4, Amar Iqbal Saqib1, Hafeezullah Rafa1, Abdul Wakeel1, Ghulam Shabir1, Muhammad Rizwan1, Muhammad Qaisar Nawaz1 and Muhammad Faisal Nawaz1

...aveolens) and Kalwanji (nigella sativa), under dual stress of ECe (electrical conductivity of soil extract) 0.79, 6 and 8 dS m-1 and SAR (sodium adsorption ratio) 5.99, 25 and 35. Each crop was grown for four months and biomass yield data was recorded. Results of three successive seasons suggest that all the evaluated medicinal plants can grow under the medium salinity and sodicity level of (6 dS m-1 + 25 SAR). However, biomass yield decreased linearly with in...

Husmaini1*, Sabrina1, Firda Arlina1, Linda Suhartati1, Yozella Martilova2 

...g the microflora in the digestive tract when consumed in sufficient quantities. Purple sweet potato has high effectiveness against LAB growth, namely 10,771 (log10 cfu/g) with 85.34% resistance be used as a medium for LAB growth. This study aims to determine the effect of probiotics Lactococcus plantarum and Pediococcus pentasaceus using a purple sweet potato carrier on laying hen’s performance and egg quality. This study used 210 layers of medium-type ...

Razaq Adekunle Animashahun1*, Gbenga Emmanuel Onibi2, Samuel Olanrewaju Aro2, Oghenerobor Benjamin Akpor3, Funmilayo Abimbola Okeniyi1, Olayinka Olubunmi Alabi1, Michael Babatunde Falana1, Ayoola John Shoyombo1, Samuel Oyewale Olawoye1 

...tumps using Aspergillus niger (ATCC 16404) strain in a solid substrate. The dried and milled cassava stumps were aseptically inoculated with Aspergillus niger (ATCC 16404) strain in a solid-state at substrates to water ratio 1.0:1.0 w/v and then incubated for 192 hours at room temperature. Samples were taken at 48 h interval for the proximate, minerals and anti-nutrients composition determination of the fermented cassava stu...

Caiyun Ma1, Hebao Wen1, Lu Li2, Gaofeng Liu1, Chunjing Wang1, Yu Guo1, Shiji Fang1, Hongyu Wang1* and Changqing Liu1*

...ed recognized surface antigens of human CSPCs. Additionally, CSPCs exhibited differentiation multipotency, including osteogenic, adipogenic and chondrogenic potential in vitro. These findings may propell CSPCs application in a clinical study, suggesting potential therapeutic treatment for OA or related joint diseases.

...

Anara Ryskeldina, Indira Iskakova, Nurgul Sarina , Alexander Shevtsov , Laura Syzdykova, Alexander Shustov, Yerlan Ramankulov, Marat Kuibagarov* 

...ycoprotein 1 (boPAG1) antigen. The aim of this work was to produce a recombinant boPAG1 antigen and obtain monoclonal antibodies (mAbs) against boPAG1. We have obtained the boPAG1 cDNA and are reporting its nucleotide sequence. Bacterial expression of a portion of the natural gene encoding a mature form of boPAG1 failed. But a fusion protein made up of E. coli thioredoxin and boPAG1 was efficiently expressed in E. coli. Usin...

Iram Amin1,2*, Shazia Rafique1, Nadeem Ahmed1, Muhammad Shahid1, Samia Afzal1, Tahir Rehman Samiullah1, Mohsin Ahmed Khan1 and Muhammad Idrees1,3

...ve of the study. This antigenic recombinant HDAg protein can be useful for both vaccine development and as a diagnostic marker of HDV. After determination of HDAg antigenic region of HDV and its amplification, the fragment was cloned in bacterial expression vector. The expression of recombinant protein was checked by SDS-PAGE and after the purification of protein by using Ni-affinity column. antige

Muhammad Ismail1, Jabbar Khan1*, Zahid Rauf2, Khalid Khan3 and Muhammad Rafi1

...Amplified products were digested with Xbal and Pvull restriction enzymes and were electrophoresed to visualize single nucleotide polymorphisms (SNPs); rs9340799 (G>A) and rs2234693 (C>T) in ESR1 gene. The amplified product of 524bp of rs9340799 sequence of ESR1 gene was treated with Xbal restriction enzyme while amplified product of 451bp of rs2234693 sequence was treated with PvuII restriction enzyme. Nine patients were found homozygous and 8 were heter...

Olufemi Bolarin, Sijuade Adebukola Adebayo and Sola Emmanuel Komolafe*

...farmers in Benue State, Nigeria was investigated in this study. One hundred and eighty (180) yam farmers were sampled for the study. Primary data was gathered with the use of interview schedule. Descriptive statistics and regression were used to analyze data collected. The result shows that the mean age of the farmers was 45.3 years and 83.3% were males. The result also shows that weather forecast (Mean=1.42) and change in farming type (crop- livestock Mean=1....

Onwusiribe Ndubuisi Chigozirim*, Okpokiri Chibuzo Ikechukwu, Agu-Aguiyi Fortune Nneka and Oteh Ogbonnaya Ukeh

...er farmers in southeast Nigeria. The policies, programs, projects, activities of private and public organizations to intervene on climate change-related issues are referred as institutional interventions. The study therefore, identified the institutional interventions and support available for farmers affected by climate change. In addition, identified the climate-smart practices used by the smallholder farmers, analyzed the relationship of the socioeconomic v...

Paulus Klau Tahuk*, Oktovianus Rafael Nahak, Gerson Frans Bira 

...mption of nutrients and digestibility of feed, as well as cattle growth performance which includes daily body weight gain, conversion and feed efficiency. The results of this study showed that the consumption (kg/head/day) of DM, OM,, CP, CF and energy consumption (Kcal/g/DM) were relatively the same between treatment. Digestibility (%) of DM, OM, CP, CF and energy were relatively the same between treatment. The T3 treatment...

Sadaf Ansari1, Shahid Hussain Abro1, Abdul Jabbar Tanweer2, Abdullah Sethar3, Ghulam Abbas4, Sabahat Ansari1, Asghar Ali Kamboh1* 

... (15%, 10% and 0%) and Shigella dysenteriae (10%, 12.5% and 5%) in beef, mutton and fish respectively. While from cooked beef, mutton and fish the prevalence of E. coli (25%, 25% and 20%), S. aureus (15%, 15% and 10%), S. enteritidis and B. cereus (10%, 10% and 10%) were observed. The highest (p < 0.05) bacterial load (g­¹) was detected in raw (1.76×106) and cooked (6.1×104) beef than raw (1.55×106) and cooked (4.5×104) mut...

Aulia Nurul Saputri, I Komang Gede Wiryawan, Dilla Mareistia Fassah*, Dewi Apri Astuti 

...f LAB isolated from the digestive tract of black soldier fly (BSF) larvae on different substrates as probiotic candidates. The LAB from the digestive tract of BSF larvae in chicken manure and palm kernel meal substrates was isolated and taxonomically identified using the 16S rRNA sequence homology and molecular identification. The LAB isolates were also tested for antimicrobial and hemolysis activities. Five isolates from BS...

Ali Ahmad1*, Zubair Aslam1, Korkmaz Bellitürk2, Ehsan Ullah1, Ali Raza1 and Muhammad Asif3

...microbes collaborate to digest solid agricultural waste under controlled circumstances, resulting in a stable form of organic matter. Earthworms produce vermicompost, which contains growth-regulating hormones, macro-micro nutrients, plant-immobilized microflora, growth regulators and promoters and cellular portion degrading enzymes (cellulose, proteases, lipase, amylases, chitinase), which degrade organic substrates even after they have been secreted. Earthwor...

Othman E. Othman1*, Lingjiang Min2, Amira M. Nowier3  

...ned to investigate the epigenetic regulation of ovulation rate through DNA methylation profile using WGBS. The whole genome DNA was extracted from ovaries of Zaraibi goats belonging to two different fertility groups: high (HFG) and low (LFG) fertility groups. The extracted DNA was subjected to WGS after treatment with bisulfite. The findings declared that a small difference in the DNA methylation levels is present among high and low fertility groups. The methy...

Hossam Mahrous Ebeid1, Ahmed Abdelkader Aboamer1*, Amgad Ahmed Abu Elella2, Ibrahim Mohamed Khattab3, Osama Hefny Matloup1, Fatma Ibrahim Hadhoud1  

...as thought to be highly digestible due to its high ruminally degradable fraction (76.1%). Increasing supplementation amount of DMSC in goats’ diets reduced their blood cholesterol (linear; P = 0.012). However, total protein, albumin, globulin, urea-N, glucose, and liver enzymes showed no significant changes. DMSC supplementation increased milk energy content (MJ/kg), milk total solids, non-fat solids, protein, and lactose (g/kg) (linear; P < 0.05). In...

Sekobane Daniel Kolobe, Tlou Grace Manyelo*, Jones Wilfred Ng’ambi, Munyadziwa Felicia Dorcus Nemauluma, Emmanuel Malematja  

...t (P>0.05) gut organ digesta pH, lengths, and weight values of male and female broilers aged 21 days. At day 42 of age, AKLM inclusion levels in the diets affected (P<0.05) gastrointestinal tract (GIT), ileum, and large intestine lengths of male broilers. Female broiler chickens on a diet having 1.0g of AKLM had longer (P<0.05) GIT than those without AKLM inclusion. Female broiler chickens on a diet having 1.0g of AKLM per kg DM had higher (P<0.0...

Marat Kuibagarov1, Assylbek Zhylkibayev1, Dinara Kamalova1, Anara Ryskeldina1, Nurdina Yerzhanova1, Yerlan Ramankulov1, Alexandr Shevtsov1*, John A Angelos2  

...ilin is one candidate antigen for M. bovoculi, and is a key pathogenic factor necessary for attachment of M. bovis to the surface of the eye epithelium. To evaluate pilA diversity amongst Kazakh isolates of M. bovoculi, the pilA gene sequence was determined for 35 isolates of M. bovoculi. Ten genotypes (gNA1-gNA10) were identified which were 96.1% to 99.8% identical. It was found that as a result of the replacement of cytosine with thymine at the 97th positio...

Ekechukwu Esther1, Ohanu Chinenye1, Ossai Nelson1*, Elijah Okwuonu1, Hinmikaiye Funmilayo1, Ngene Innocent1, Andong Felix1, Ikegbunam Clara2, Echude Daniel1, Ekeh Felicia1 and Odo Gregory1

...jan on growth, nutrient digestibility and biochemical indices of 54 Giant rats were studied for 42 days department of zoology laboratory of University of Nigeria, Nsukka. The rats were divided randomly into six groups of 0%, 10%, 20%, 30%, 40% and 50% C. cajan diet respectively, with a total of 9 giant rats in each group. The diets contained protein, fibre, ash, fat and sugar /energy. Feed efficiency increased in the rats fe...

Jie Yang1, Wenjun Zhou2, Qi Zhang2, Lei Chu2, Zhenshi Chen1, Xiajun Zhang2, Weidong Wu3*, Shaoru Zhang1* and Lihui Wang1*

...y N. gonorrhoeae multiantigen sequence typing (NG-MAST). 388 clinical isolates of N. gonorrhoeae were identified, of which 373, 329, 298, 5, 11 and 5 strains were resistant to ciprofloxacin, penicillin, tetracycline, AZM, spectinomycin and ceftriaxone, respectively. The mutation detection of AZM-R related gene showed that there were single (A) nucleotide deletion mutation in mtrR promoter region, G45D mutation in mtrR coding region, G70 mutation in rplD and A2...

Hamenya Mpemba1, Fan Yang1, Kirsty J. MacLeod2,3, Dusu Wen1, Yan Liu1,4 and Guangshun Jiang1,*

...rs (brown bear and Amur tiger, respectively) influence ungulate, mesopredator, and top predator visitation rates to camera trap sites in a national nature reserve in China. The comparison of these predator types is of particular interest in this region as Amur tigers may shortly be reintroduced here. We found that visual but not odour cues significantly affected ungulate visitation rates: ungulates showed reduced visitation ...

Rani Winardi Wulan Sari1, Novirman Jamarun2*, Suyitman3, Khasrad4, Elihasridas2, James Hellyward5, Gusri Yanti1 

...utrient and cells fiber digestibility, rumen fluid characteristic, and gas production using an in vitro methodology. Samples of Hay Mangrove Leaves (HML) (Rhizophora apiculaca) and Native Grass (NG) were arranged into dietary tretmeants (Dry Matter (DM) Basic). The experimental design used is a randomized block design consisting of 4 treatments with 5 replications. The treatment was P1=60% Hay Mangrove Leaves (HML)+40% Native Grass (NG), P2=50% HML+50% NG, P3=...

Murad Ali Rahat1*, Muhammad Israr2, Hakim Khan1, Ishtiaq Hassan1, Mohammad Islam1 and Muzafar Shah3

...ign: justify;">DNA Intelligence is relatively new concept which relies on providing phenotypic information about unknown individuals when traditional DNA profiling is not informative. The system aids in forensic investigation by probabilistic prediction of eye, hair, skin colors as well as other phenotypic features. The Irisplex system contains a very sensitive multiplex genetic typing test that allows users to predict blue, brown and intermediate human eye co...

Tlou Grace Manyelo1,2, Nthabiseng Amenda Sebola1, Jones Wilfred Ng’ambi2, Monnye Mabelebele1* 

...and gut morphology of indigenous Boschveld chickens. A total of 200 one-day-old indigenous Boschveld chicks were randomly allocated to five treatments in a completely randomized design each with four replicates of ten chicks. Amaranth leaf meal (ALM) levels evaluated in this study were 0, 5, 10, 15, and 20% with recording weekly body weights, feed intakes, and feed conversion ratios. Gut organ weights, lengths, pH, and meat ...

Adefunke Fadilat O. Ayinde1*, Peter Allison Johnston2, Olanrewaju Olusoji Olujimi3, Purnamita  Dasgupta4 and Dare  Akerele5

...ty in two ecosystems in Nigeria. Climate data (spanning 1951 to 2010) were used to corroborate and evaluate the perceptions of farmers. Four hundred smallholder farmers were interviewed in Ogun State (rain-forest zone) and Kwara State (derived savannah) using a multi-stage sampling technique. Farmers perceived climate variability as unpredictable weather situation over the years (65.41%), though some (23.31%) perceived it as the act of God or the wrath of God ...

Muhammad Shuaib1*, Abdul Hafeez1, Naila Chand1 and Muhammad Tahir2

...erformance and nutrient digestibility during peak egg production period with different phases (phase-1=week 29 to 32, phase-2=week 33 to 36, and phase-3=week 37 to 40). One hundred and sixty golden misri (Brown) laying hens of age 28 weeks were brought for the experimental purpose and reared up to 40 weeks of age. All birds were divided into 4 groups with 4 replicate per group containing 10 birds per replicate and offered the corn-soybean meal diet with soybea...
Abdullah Iqbal1, Muhammad Abubakar2, Shumaila Manzoor3, Muhammad Kamran Ameen4 and Rani Faryal1*
 
...A from serum samples. Antigen shedding by vaccinated animals via nasal and fecal route was checked by the haemagglutination test and RT-PCR. All group 2 bucks, developed humoral protection against PPR within the 1st week. Only, 50 percent bucks of group 1 developed humoral protection against PPR after one week of vaccination. The mean percent inhibition value of competition-ELISA of group 2 was half the mean percent inhibition values of group 3 and group 4 aft...

Istna Mangisah1*, Heni Rizqiati2, Nyoman Suthama1 

...p>Maintaining a healthy digestive tract is important for poultry production. Intestinal microbial balance is beneficially influenced by either probiotics or prebiotics as well as their combination. This study aimed to investigate the feeding effect of Lactobacillus casei and dahlia tuber extract (LDTE) mixture on intestinal microbial, digestive and immune organs weights, nutrient digestibi...

Muhammad Bilal Munawar1, Saima Naveed1, Shafqat Nawaz Qaisrani1, Jibran Hussain2, Shahzad Munir3, Ayesha Ahmed3, Mubbashir Hussain4, Ahmad Kamran Khan5, Abdullah Jalal6 and Irfan Ahmed7*

...roduction. Blood, ileal digesta, weight of visceral organs, left tibia were sampled for the quantity of phosphorus in plasma, its digestibility and tibial calcium and phosphorus. The present study reported that H group was found higher in FI, BWG and FCR as compared to other groups. Higher gizzard, liver and heart weight were recorded in group A and B while tibia ash, Ca and P in group H. Described results concluded that sup...

Sara Magdy Hashim1, Elshaimaa Ismael2, Mohamed Tarek3, Faten Fathy Mohammed1*, Fatma Amer Abdel Reheem3, Rawhia Esawy Doghaim1 

...acterization of viral antigen revealed direct relation between viral residence in tissue and developed pathology. The present study confirmed that H5N8 HPAI clade 2.3.4.4b, became a predominant strain during the period 2018-2020, causing severe outbreaks in duck farms in Egypt.

Keywords | Avian influenza, HPAI, H5N8, Ducks, Pathology, Egypt. 

...

Arif Mehmood1*, Muhammad Naeem2, Abu Bakar Muhammad Raza1, Muhammad Asam Riaz1, Muhammad Zeeshan Majeed1, Nadra Khan3 and Waqas Raza4

...a (2), Aedes (2), and Armigeres (2) and deposited in the Biosystematics Laboratory of Pir Mehr Ali Shah, Arid Agriculture University Rawalpindi. Quantitative habitat webs and diversity indices show that scrapyard, animal sheds, and residential areas were the most abundant habitats respectively, while areas near stream were found to be the least abundant habitats. These microhabitats, which are the most abundant ones act as hotspots in case of mosquito-borne ep...

Kamel Etman Ibrahim Etman*, Salah Kamal Sayed, Farouk Amin El-Sayed, Abd-Alghany Hasaneen Ghoniem, Mostafa Mohamed El-Nahrawy, Mona Ahmed Alsayed Farag 

...itives were studied on digestibility coefficients, feeding values, , feed intake, daily gain and economical efficiencies with using thirty male buffalo calves in fattening trial. Rumen and blood parameters were also studied. First group (10 animals) were received control ration which contains 60 % concentrate feed mixture (CFM), 30 % corn silage (CS) and 10 % alfalfa hay (AH). The 2nd and 3rd animal groups were fed control ration supplemented with 5 gm prob...

M. Mansoor Javed*, M. Aslam Mirza, M. Aziz ur Rehman and M. Saif ur Rahman

...ate composition, pepsin digestibility, KOH solubility and urease activity prior to inclusion in the experimental diets. A day old Cobb-500 broiler birds (n=432) were purchased commercially. Nine iso-caloric as well as iso-nitrogenous experimental formulations as starter diets (CP 22 %, ME 3000 Kcal/kg), grower diets (CP 20.5 %, ME 3075 Kcal/kg) and finisher diets (CP 19 %, ME 3150 Kcal/kg) were formed as per nutritional recommendations for Cobb-500 and assigne...
Amber Iqbal1, Mahroze Fatima2, Syed Z. H. Shah3,*, Muhammad Afzal1, Moazama Batool4, Muhammad Bilal5, Naheed Bano6, Ayesha Khizar2 and Sumbal Iqbal7
... Labeo rohita during refrigerated storage was investigated. Six graded levels of vitamin E, between 0-125 mg/kg vitamin E, were fed to fish. After 60-day feeding trial, fish were sacrificed, fillets were stored in refrigerator at -20°C and analyzed on 0, 15 and 30th day of storage. Upon refrigeration the vitamin E fed fish showed reduced level of Thiobarbituric acid reactive substances...

Yar Muhammad Jalbani1,2*, Muhammad Farooque Hassan2, Kiran Nazish2 and Yang Shuming1

...ed even stored under refrigeration conditions. It is a challenging task to accurately estimate the storage life of chilled chicken meat to avoid economical losses and provide the basis for standard regulations assessing meat storage life. For analyzing the storage life of chilled chicken meat rapidly, the samples were analyzed on Day 0, 3, 5, 7 and Day 10 using near infra-red spectroscopy approach combined with chemometrics tool of data driven soft independent...

Rahman Ullah1*, Muhammad Junaid2, Nabila Gulzar2, Rahat Ullah Khan3, Baseer Ahmad4, Ambrina Tariq5, Aamir Iqbal6, Mushtaq Ahmed7 and Mirwaise Khan8*

...fication) ETEC (Enterotoxigenic Escherichia coli) strains in both raw and pasteurized milk available in the market of District Kasur, Punjab (31.0896° N, 74.1240° E). A total of 65 samples of milk including 5 pasteurized milk samples from various sources were analyzed through scientific polymerase chain reaction (PCR) method including other microbiological laboratory techniques (Total plate count, Gram staining, Hydrogen sulphide, Citrate, Urease and I...

Khalid Farooq, Muhammad Tahir and Nazir Ahmad Khan*

...abolizable energy (ME), digestibility of DM and nutrients, body weight (BW) gain and body condition score (BCS) were recorded. The intake of all nutrients improved (P < 0.05) with the incorporation of blends of maize silage and ryegrass in the diet. Moreover, the intake of DM, organic matter (OM), and ME consistently increased (P < 0.05) with increasing maize silage inclusion levels (30 to 70%) in the forage blends, and the highest (P < 0.05) intake w...

Muhammad Saif-ur Rehman1, Faiz-ul Hassan1* and Muhammad Sajjad Khan2

...relationship among 10 indigenous cattle breeds (Sahiwal, Red Sindhi, Cholistani, Dajal, Dhanni, Rojhan, Lohani, Hissar, Hariana and Tharparkar) were analysed using RAPD markers. A total of 80 RAPD primers were used for PCR amplification of DNA from the cattle breeds. The PCR products were analyzed by agarose gel electrophoresis. Scoring of bands were performed to generate dendrogram using unweighted pair group method of arithmetic means (UPGMA). Six hundred an...

Saba Rehman1, Faisal Salih Hayat1, Sadia Norin2, Abdul Aziz1, Siddiq Ur Rahman1* and Noor ul Haq1*

... found to the highest antigenicity score. The present study identifies natural inhibitors and potential antigenic Epitopes which may be used as effective drug and vaccine candidates to suppress the novel coronavirus.

 

...

Umar M. Bello1*, Samuel A. Ojo1, Abdurrahman Ghaji1, Ambrose A. Voh (Jr)2, Muazu N. Bappah3, Casmir O. Igbokwe4  

...or of oestrous stage in Nigerian indigenous breeds of Red Sokoto does.

Keywords | Exfoliative vaginal cytology, Oestrus cycle, Red Sokoto does, Rectal temperature, Vaginal temperature 

...

Müge Aliye Hekimoglu and Sırma Sönmez

...y of the experiment for digestive enzymes (protease, lipase, amylase), weight and length, body colour and survival rate. The image analysis method was used to determine skin colour. The chemical analysis method was used to determine the activity of enzymes. The results showed that supplementation of 2000 mg/kg of dietary significantly increased weight, length and specific growth rate (P < 0.05). While no change in colour was observed in the control group at...

Nauman Zaheer Ghumman, Muhammad Ijaz*, Arslan Ahmed, Muhammad Umar Javed, Iqra Muzammil and Ahmed Raza

...n=172) collected from indigenous bovines of district Rawalpindi, Pakistan. The milk samples were screened for S. aureus and the confirmed isolates were subjected to disc diffusion test, PCR, and SDS-PAGE analysis for the confirmation of methicillin resistance. The farm-associated and individual animal-associated risk factors were analyzed to check the association with S. aureus and MRSA-associated subclinical mastitis. The results revealed an overall molecular...

Oluwatoyin Adenike Fabiyi1*, Mariam Temitope Baker2 and Gabriel Ademola Olatunji2

...etable of importance in Nigeria which is often times infested with root-knot nematodes (RKNs), thus reducing yield and expected income. Principally, synthetic nematicides are employed in the management of RKNs on agricultural fields. The synthetics are confronted with a web of regulations on account of their unhealty negative effect on humans and the environment. Plant protection is primarily saddled with replacing the synthetics. A promising technique is the ...

Siti Dharmawati1, Muhammad Halim Natsir2, Hartutik Hartutik2, Danung Nur Adli2, Osfar Sjofjan2*  

...ively organ weight, and digestibility of Alabio ducks (Anas plathyrhyncos borneo). A total of 210 one-day old ducks male Alabio with an average body weight of 45.26±2.59 g were placed in colony-shaped pen, and each pen contained seven ducks. The trial design used was completely randomized design with five treatments and six replications for each one, consisting of seven ducks. The treatment diet was formulated as follows: T0 basal diet + 100% fish meal ...

H. Sagir and Y. I. Erum†

...ed in this paper. The indigenous fauna had similar morphological and morphometrical
characters to the corresponding type population.
...

M. S. Bajestani1, K. Dolatabadi2†, E. Mahdikhani-Moghadam1

...div>officinalis L.) and nigella (Nigella sativa L.) on root-knot nematode, Meloidogyne javanica @ 1500, 2500 and 5000
juveniles were studied on susceptible tomato cv. Karoon under greenhouse condition. Although all the treatments
reduced root infections rate and significantly effect on root weight (P ≤ 0.05). It was observed that when the
nematode inoculum increased, reproduction factor...

Ahmed S.M.H. EL Roby

... the efficacy of four indigenous and one exotic isolated entomopathogenic
nematodes (EPNs) against sugarcane mealybug Saccharococcus sacchari under laboratory conditions (27 ± 2 °C,
RH 65 ± 10%). The EPNs used in the study are Steinernema sp. (strain: AT4), Steinernema sp. (strain: EMB),
Heterorhabditis bacteriophora (strain: EKB20), Heterorhabditis sp. (strain: EIK) and Steinernema glasri (strain:
<...

M.A.M. El-Saedy, A.A. Mokbel*† and S.E. Hammad**

...amphor, castor, garlic, nigella, onion
and sesame resulted in 48.0-92.7% reduction in numbers of root galls/root under laboratory conditions. Similarly,
application of these oils resulted in great reductions of 59.2-92.8% and 47.4-89.6% in numbers of root galls and
egg-masses/root when oils applied before and after transplanting of tomato seedlings, respectively. The highest
reduction...

F. Shahina†, K.A. Tabassum and M.A. Habib*

...nd efficacy trials of indigenous
entomopathogenic nematodes (EPNs) isolates have positive results. Four EPN isolates viz., Steinernema
pakistanense, S. asiaticum, S. feltiae and Heterorhabditis indica were assessed for their infectivity against the
cotton bollworm complex in field. EPNs cultured on Galleria mellonella L. and stored in distilled water at 5 °C,
were kept at room temperature for 24 hrs before us...

Farhana Binte Zalal, Prodip Kumar Sarkar*, Mahbuba Sultana, M H Kawsar, Swapon Kumar Fouzder 

...duction and they used indigenous hens to incubate eggs. An average egg number per clutch was 12.6 and farmers set 12.3 eggs per broody hen for incubation purposes. The hatching egg weight was 37.3 g with egg hatchability of 87.9%. We found a significantly low body weight of broody hens after egg incubation. A high percentage of farmers (42%) preferred the spring season compared to the summer and rainy seasons to incubate eggs. For the selection of broody hens,...

Belema Robert1, Favour Welenya1, Deborah Achi1, Cynthia Onyeagwara1, Soala Obie Minimah2, Ebele Anulika Obichi2, Chidinma Charity Amuzie1* 

...rt Harcourt metropolis, Nigeria. Methodology: Vegetables (fluted pumpkin leaves, waterleaves, bitter leaves and scent leaves) were purchased and harvested from selected markets (Creek Road, Mile 1, Mile 3, Timber, Rumuokoro, and Rumuokuta Markets) and farms (Rivers State University Agricultural Demonstration Farm, a farm at Nkpolu-Oroworukwo and another at Rumuolumeni) in Port Harcourt metropolis in November and December, 2021. Samples were taken biweekly and ...

Soshe Ahmed1*, Mst. Ishrat Zerin Moni1, Maksuda Begum2, Md. Jafar Eqbal3, Md. Shahidul Islam4, Mst. Samira Tanjim1, Mst. Rokeya Sultana1 

...train NLT 106.0 EID50 antigen. Post-hatch feed and water deprivation durations significantly affect the production parameters. Feed intake, body weight gain and average daily gain were significantly (p<0.01) lower in the T5 group. Significantly (p<0.01), a poor feed conversion ratio was observed in the T5 group. The lowest (91.66%) survivability (p<0.01) was found in the T5 group compared to other groups. There was a significant (p<0.01) difference...

Yuejing Yang1,2, Mengbin Xiang1,2, Zhengshi Zhang3, Minrui Zhou1, Bingjie Hu1, Tingsen Jing1, Zhe Li1, Fenglin Liu1, Hui Luo1, Qinglu Li1* and Hua Ye1*

...0% of the variance, the eigenvalue was greater than 1, and the accumulative variance was more than 85%. So, the body weight was the first principal component of the growth traits for S. prenanti. Association analysis indicated that ug25066-1502 had significantly associated with total length and body weight; and ug22539-1605 and total length, body length, body height, and body weight were significantly associated. In conclusion, ug25066-1502 and ug22539-1605 we...

Muhammad Iqbal Anjum*, Shahbaz Javaid, Agha Waqar Yunus, Faisal Ashfaq and Javed Iqbal

...eight gain and nutrient digestibility during last 5-days were recorded of individual animals. The calves fed TMR with 25% MC attained significantly higher weight gain (830 g/d, P<0.05), followed by the calves fed TMR with 35% MC (760 g/d) whereas, those fed TMR with 35% MS got lowest weight gain (687 g/d). Similarly, better feed conversion ratio (6.63) was observed in calves fed 25% MC based TMR while poor (7.90) FCR was observed in TMR with 35% WS. Intake ...

Ronny A.V. Tuturoong1*, Sony A. E. Moningkey1 and Nova L.I.M. Ogi2

...), acid detergent fiber digestibility (ADFD), NH3 synthesis and efficiency of microbial protein synthesis were analyzed. The highest dry matter digestibility (DMD), organic matter digestibility (OMD), neutral detergent fiber digestibility (NDFD), NH3 concentration and efficiency of microbial protein synthesis (ESPM) were found in T2 group (64.04%, 65.05%...

Kalsoom1, Nasir Shah2, Muhammad Ibrahim3*, Tahira Bibi1, Kazim Ali4 and Zahir Shah2

...ties were grown on Murashige and Skoog (MS) media subjected to (0, 25, 50, 75 and 100 mM) of Sodium Chloride (NaCl). Potato varieties showed an adverse in vitro growth response to all levels of salt in MS media, while plants of all the tested varieties were dead at concentrations more than 50 mM of NaCl in in vitro condition (75, 100 mM). Variation was observed in the level of tolerance to salinity in these potato varieties. Increasing NaCl (mM) level reduced ...

Malik Muhammad Yousaf1, Wali Muhammad1, Muhammad Mohsin Raza1*, Mumtaz Hussain1, Muhammad Jahangir Shah1, Bashir Ahamad1, Annum Sattar2, Hera Gull3, Sonia Sumreen4, Malik Waqar Yousaf5, Nazakat Nawaz6 and Nazim Hussain7

...rst three PCs reflected eigen values more than one where cumulative value was 61.78% of the total variability. The first one accounted as highest proportional value of total variability in all PCs and in remaining PCs lesser amount of variation. High values of heritability referred to broad sense genetic advance possessing higher values in plant attributes under study witnessed that these parameters abide by the effects of additive genes, concluding that sorti...

Malik Muhammad Yousaf1, Wali Muhammad1, Muhammad Mohsin Raza1*, Mumtaz Hussain1, Muhammad Jahangir Shah1, Bashir Ahamad1, Annum Sattar2, Hera Gull3, Sonia Sumreen4, Malik Waqar Yousaf5, Nazakat Nawaz6 and Nazim Hussain7

...rst three PCs reflected eigen values more than one where cumulative value was 61.78% of the total variability. The first one accounted as highest proportional value of total variability in all PCs and in remaining PCs lesser amount of variation. High values of heritability referred to broad sense genetic advance possessing higher values in plant attributes under study witnessed that these parameters abide by the effects of additive genes, concluding that sorti...
Ulvi Fitri Handayani1, Wizna2, Irfan Suliansyah3, Yose Rizal2, Maria Endo Mahata2*
...tion of lycopene in the digestive tract of laying hens. Lycopene is a compound in tomatoes and is known to inhibit cholesterol synthesis. Cholesterol in the body is transported in the blood by lipoproteins. Therefore, that is important to know the effect of giving processed tomatoes wastes (PTW) on laying hens to lipid profile of blood serum consist of cholesterol total, triglycerides, LDL (Low Density Lipoprotein), and HDL (High Density Lipoprotein). Two hund...
Rafik Soliman1, Neven Waheeb2, Essam Nasr2, Mahmoud El-Hariri1, Heidy Abo-Elyazeed1, Hassan Aboul-Ella1*
...ostic techniques. IQRT Anigen Rapid Bovine TB Ab kit and Ubio quick VET Bovine Tuberculosis Antibody kit were used in testing 3750 dairy cattle. The results obtained were compared with the ELISA, bacteriological examination, and tuberculin skin testing. By the tuberculin test, 69 (1.8%) animals reacted positively, from which 51 animals (73.9%) showed visible lesions on postmortem examination while the remaining 18 animals (26.1%) showed no visible lesions. Usi...
Shaker Al-Suwaiegh
...lla pinnata on nutrient digestibility, growth performance and blood metabolites of growing Ardi goats were explored. Twenty one growing Ardi goats, 27.0 ± 0.40 kg body weight and aged 4.0 - 5.0 months, were randomly allocated to three equal groups (seven/group) as Azolla pinnata (10.0 and 20.0 % daily feed) and control groups. The trial lasted 12 weeks. Feed intake, feed efficiency, and body weight gain were determined. In addition, nutrients’ d

Tilahun Bekele* and Abebaw H. Georgis

...notypes of black cumin (Nigella sativa L.) in response to seed rate. The experiment composed of four varieties (Dirshaye, Darbera, Aden, and local), and four levels of seed rate (10kg, 15kg, 20kg, and 25kg per ha) set out in a randomized complete block design and replicated three times. The findings of the statistical analysis exhibited that the majority of the response variables under consideration were significantly (P<0.01) influenced by seed rate, varie...

S.H.M. Faruk Siddiki1*, Md. Golam Morshed2, Md Robiul Karim1, Lutfun Naher1, Md. Sodrul Islam3

...stemic states (20.12%), digestive disorders (18.55%), gyneco-obstetrical diseases (4.37%), and surgical cases (3.06%) were recorded as major clinical problems, with other diseases having a prevalence of less than 1% for each. Among the ten categories, the prevalence of parasitic diseases, infectious diseases, general systemic states, and digestive disorders was significantly (P < 0.001) higher. The diseases were significa...

Roni Pazla1, Novirman Jamarun1*, Elihasridas1, Arief1, Gusri Yanti2, Zaitul Ikhlas2

...rude protein), nutrient digestibility (dry matter, organic matter, and crude protein), and production performance (body weight gain and feed efficiency). The result showed that the difference in ration composition had a significant effect (P<0.05) on feed consumption, nutrient digestibility, and production performance. This study concluded that the combination of 35% fermented sugarcane shoots +14% fermented tithonia + 1%...

M.M. Mikailov1*, O. Yu. Chernykh2, E.A. Yanikova1, G.A. Nurlygayanova3, O.D. Sklyarov4 

...on reaction with O-PS antigen (RID with O -PS antigen). The specificity of these serological tests was confirmed by the negative results of studies of 40 blood serum samples from animals from a safe farm not vaccinated against brucellosis. Determination of the sensitivity of the tested diagnostic tools was carried out using 46 blood serum samples from cows immunized with a vaccine from the B. abortus 82 strain from a farm th...

Hasnain Raza1,2, Qun Liu1*, Muhammad Tariq Hanif2 and Yanan Han1

...rson and Parastromateus niger were overfished and Lutjanus argentimaculatus was strongly overfished. However, all the stocks were subjected to overfishing. Therefore, the parameters estimated from our study may be used as indicators for the sustainable management of the fisheries in the country.

...

Dalia Zaafar1*, Heba M.A Khalil2, Soha Hassanin3, Mohamed R. Mousa4, Mona G. Khalil1 

...ferating cell nuclear antigen (PCNA), hypoxia-inducible factor 1-alpha (HIF-1), PPAR, and cyclin D1. Results: Mice treated with Cis+Pio HD showed higher levels of SOD, GSH, p-ERK1/2 and p38-MAPK, as well as lower levels of MDA and tumor growth factor beside the higher apoptotic activity. Furthermore, Cis+Pio HD reduced the expression of PCNA and HIF-1 and increased the expression of PPARγ, which improved the histopathological changes. Conclusion: Pio pos...

Shabana Noureen1, Shirmeen Ijaz2, Saad Ullah3, Iqra Shafique Abbasi4 and Shakeel Ahmad5*

...ating breaking news and digesting bite-sized content and communicating directly with the users in real-time. Currently, it has been used to communicate mental illnesses as well. To examine symptoms and major themes of social anxiety discussed on Twitter A qualitative research design was used in the current research. NCapture was used to randomly select 500 tweets of social anxiety published from 1st January 2022 to 30th June 2022. Nvivo was used to do a conten...

Nguyen Thi Kim Dong1*, Nguyen Van Thu2 , Nguyen Hoang Qui3 

...he first factor was the digestion technique (ileal and total tract digestibility) and the second factor was the diets. Four diets included a basal diet (SBP0), which consisted of fish meal 20% (FM), broken rice 80% (BR) and the other three diets, in which the content of FM was 25.0, 50.0 and 100% of the FM level in the basal diet and was replaced by ensiled shrimp by-products corresponding the SBP1, SBP2 and SBP3 diet, resp...
 
 MUHAMMAD WAHEED MUSHTAQ1*, NAUMAN JAMIL KHAN1, NAVEED ASLAM DOGAR1, AISHA HAMEED2, ABDUL HAMEED2, MALIK ADIL2, HAROON IFTHIKHAR3 & SAIRA ISHAQ
...d thirdly, stored in refrigerator (4 ºC) for 2 days. It was observed that vegetables stored at room temperature was significantly affected by loss of Vit. C as compared to refrigerated LVs. The average % age loss of ascorbic acid observed in refrigerated and room temperature stored vegetables was 34.18 % and 40.9 % respectively, as compared to fresh one. The highest concentration of v...
 
 MUHAMMAD MUDASSAR SHAHZAD1*, FATIMA KHALID1, MEHWISH FAHEEM2, SYED ZAKIR HUSSAIN SHAH3, SANA BASHIR1 & SYED MUHAMMAD KHAN
... activities and augment digestion. The present study was designed to judge the effectiveness of Tau supplemented LMS (linseed meal) based diet in enhancing mineral absorption and nutrient digestibility in selected fish fingerlings. Graded levels of the prepared feed containing (0, 2, 4, 6, 8, and 10 g/kg) of Tau were fed to C. carpio fingerlings for 60 days. Test diets were prepared using Linseed meal and other ingred...

Arief1, Roni Pazla2*, Rizqan1, Novirman Jamarun2 

...d lactose), intake, and digestibility of Etawa Crossbred Goat. The supplemented treatments were: A; control ration (60% forage (CF) + 40% concentrate (CC)), B (30% CF + 30% Tithonia diversifolia (T) + cassava leaves (CL)) + 30% CC + 10% palm concentrate (PC) ), C (30% CF + 30% (T+CL) + 20% CC + 20% PC), and D (30% CF + 30% (T+CL) + 10% CC + 30% PC). The parameters observed were milk production, milk fat, and lactose content, intake and d<...

Ahmed Abdel-Rady3*, Mohamed Karmi2, Menna_allah Youssef1, Aml M. Abdel-Ra’ouf1, Bahaaa Madkour1 

...ite-piroplasm surface antigen Targeting gene: (Tams1) revealed positive 29 (58%) animals confirmed by visualization of specific bands at 768 bP. Positive results could be detected in suspected cattle that showed positive or negative blood smear results that proved the high sensitivity of PCR test compared with the conventional method for diagnosis of bovine tropical Theileriosis. PCR proved a highly sensitive and accurate method for diagnosis of bovine tropica...
Jian-Cheng Zhai1,2, Sheng-Nan Wang1,3, Qiang-Hui Wang1, Yan-Ling Xia1, Wei-Shi Liu1, Ya-Jie Yin4 and He-Ping Li1*
...To investigate whether epigenetic regulation of the growth factors is important in growth of sika deer, female and male reindeer antlers, methylation status were evaluated using bisulfite sequencing PCR (BSP). The 5´ flanking regions of IGF1, KGF and NGF were cloned from reindeer and sika deer antlers, and the lengths were 2089bp/2107bp, 1474bp/1474bp and 865bp/865bp, respectively. Based on bioinformatics analysis, the regions of IGF1 (-50bp ~ +615bp), K...

Ahmed Ali Chandio1, Asghar Ali Kamboh1*, Nazar Ali Korejo2, Riaz Ahmed Leghari3 and Muhammad Ali Chandio4

...ongamia pinnata is an indigenous medicinal plant of Asia, which has long traditional use, having anti-inflammatory, anti-pyretic, anti-nociceptive, anti-oxidant, anti-ulcer, antihyperglycaemic and anti-microbial activities. The current investigation aimed to explore the immunomodulatory and gut microflora modulatory effects of dietary P. pinnata in broilers. Day old broiler chicks (n=125) were purchased from local hatchery and equally divided into the five gro...

Ovirup Bhushan Paul1, Shodipta Sharma Urmi2, Md. Ashraf Ali Biswas3* 

...p>Ruminants efficiently digest fibrous components resulting in high-quality milk and meat. The emerging ruminant population to meet the protein demand is one of the driving factors of increasing global greenhouse gases. The current study aimed to assess the effects of total mixed ration (TMR) and fermented TMR (FTMR) feed on digestibility, total gas production and pH of ruminants. Novel feeding techniques were implemented th...

Riswandi1*, Ali Aim1, Muhakka1, Afnur Imsya1, Agus Wijaya2

...The value of dry matter digestibility (DMD), organic matter digestibility (OMD), pH, N-Ammonia (N-NH3), total volatile fatty acids (TVFA), partial VFA, and methane production, total bacteria, and protozoa were the variables observed. The results showed that the combination of swamp forage could increase (p<0.05) DMD, OMD, TVFA, partial VFA, and total bacteria, while N-NH3, methane production, and protozoa decreased. It wa...
Lubna Anjum Minhas, Abdul Samad Mumtaz*, Muhammad Kaleem, Rooma Waqar and Jamila Annum 
...trum, Acutodesmus, and Stigeoclonium. Hence, the current study reveals that Gujar Khan, District Rawalpindi is a rich source of green algae and an ideal place for their cultivation.

...

Shaimaa M. Ahmed1, Gehan M.A. Kassem1*, Fernando Pérez-Rodríguez2, Heba H.S. Abdel-Naeem1 

...ture (25 °C) and refrigeration temperature (4 °C). The calibration curve showed a linear relationship between the turbidity measurement and bacterial cell count in TSB. Moreover, the coefficient of determination index (R2) was 0.8789 which indicates that the linear function was acceptable for reflecting the relationship between them. The growth kinetics of S. aureus obtained from Baranyi model indicated that the maximum growth rate was non-significantl...

Enas K. Abo El-Maged, Azza H. El-Salakwy, Safia A. El-Gamal and Gehn Allam

...ion endonuclease enzyme digestion. Seventeen were type 16. eight were type 18 and four were non-typeable. The highest rate of HPV-DNA positivity was found in patients with age less than 25 years, and in patients with young age at marriage (≈ 20 years). High parity and use of hormonal contraceptives were found to be associated with high frequency of HPV positivity in aborted patients. On conclusion. human Papillomavirus types 16 and 18 might have a role i...

Hayam S. Abdelkader1; Gamalat M. Allam1; T. A. Moustafa2; and M. El-Hanunady2

...a dimorphic restriction digestion profile that "as suitable for identifying the two isolates. The method described here is rapid. reliable and highly recommended to be used in such virological and pathological studies.

...

E. K. Allam1; Kh. A. El-Dougdoug1; M. A. Amer2; A. A. Abou-Zaid2; and S. M. Amin2

...lly hybridized with digoxigenin labeled PSTVd cDNA probe.

...

Riaz Hussain2*, Adnan Ihsan1*, Waqar Khan1, Shiraz Malik1, Hamza Jameel1, Hidayat Ullah3, Shahab Ahmad4, Jawad Anwar5 and Murad Ali6

Xiaoying Liang1,2, Xiaofang Jiang3, Honglin Jia1, Ru Zhang1, Li Gao4* and Qing Yang5*

...inflammatory response. Epigenetics has an impact on disease occurrence. Epigenetic regulation in T cells contributes to the development of experimental asthma, which can be treated pharmacologically.

...

Tarique Ahmed Khokhar1*, Atta Hussain Shah1, Muneer Ahmed Jamali1 and Asghar Ali Kamboh2

...stored in freezer or refrigerator and consume after certain intervals. Hence the present study was designed to evaluate the effect of chilling, freezing and repeated-thaw cycles on chemical quality of various meats. The influence of different time was observed on chemical characteristics of chilled, frozen and thawed meat against fresh meat. Proximate composition such moisture, protein, fat, ash and glycogen decreased with increasing storage period of chilled,...

Maria Endo Mahata1*, Oriyanti Br Situngkir1, Yan Heryandi2, Takayuki Ohnuma3, Yose Rizal1 

...[L]R.Br) on crude fiber digestibility, nitrogen retention, and energy metabolism in broiler chicken. The study used 30 broiler male chickens with an average body weight of 1.5 kg at five weeks of age. This study used an experimental method with a completely randomized design (CRD) of a 3x3 factorial pattern which was repeated three times. Factor A (type of pellet binder): A1 (brown seaweed), A2 (taro tuber), and A3 (tapioca flour), then factor B (dosage of pe...

Yong Bin Zhao1, Hui Song1, Guo Gong Yi1*, Xi Lian Hao1, Bai Wang2, Zhi Wen Chen2

...s of 27 feathers from 3 pigeons were measured, and cluster analysis of these samples was carried out by using the system clustering method. The results showed a significant difference between the isotope values of the rachis and barbules from the same feather. In addition, the isotope values of the rachises and barbules differed between individuals, although the food sources of the samples might be slightly different. Based on the above results, the cluster an...

Yue Guo1,2*, Hui Zhang1, Chun Sheng Wang1 and Hong Chang Zhou1

...ta were assembled into unigenes and transcripts. Finally, all unigenes and transcripts were annotated by querying the NR, Gene Ontology (GO), COG and KOG databases.A total of 71047 unigenes and 106652 transcripts were assembled. A. cantonensis was successfully annotated by the NR and GO databases, resulting in 8107 unigenes and 16105 transcripts. Meanwhi...

Seyi Olalekan Olawuyi1*, Adedotun Oluwagbenga Anjorin2, Oluwagbenga Titus Alao3, Tosin Dolapo Olawuyi3, Rachael Ajibola Ayinla3 and Rasheed Ayodele Ayinla3

...y status in South-West, Nigeria, using cross-sectional research design, with the dataset elicited from 403 farmers drawn through a multi-stage random sampling technique. Data were analyzed using frequency distribution and percentages, cross-tabulation, and food insecurity experience-based scale for the continuum categorization of farmers’ food security status. Ordinal logistic regression model was applied to estimate the effect of adoption of bio-securit...

Bambang Hartoyo1*, Tri Rachmanto Prihambodo1,2, Wahyuningsih3, Sri Rahayu1, Fransisca Maria Suhartati1, Muhamad Bata1, Efka Aris Rimbawanto1

...ota of lactobacillus on digestive tract of broilers. A database was built from previously published article from internet reporting lactobacillus as feed additives in broilers. Articles were strictly selected according to evaluation of title, abstract and parameter which used in the study. Database collected was statistically analyzed using the mixed model method with different study as random effect and level of lactobacillus as fixed effect using SAS program...

Saima Mehar1, Amtul Sami2 and Erum Iqbal3*

...p. (Cuminum cyminum and Nigella sativa) in methanol, chloroform, ether, butanol, and hexane on egg hatching and mortality of the juveniles of root-knot nematode Meloidogyne javanica. After 72 hours, C. cyminum (MeOH) demonstrated the maximum nematicidal activity in egg hatching (15%±2.10) of M. javanica, followed by N. sativa (Ether), 22.33±6.86%, at the lowest administered dose of 0.25 % concentration. After 72 hours of treatment, C. cyminum (Me...

Rogia SA Gomez*, Said H Mbaga 

...pport, finances, and negligence still remain and need to be addressed to reduce the incidence of disease outbreaks and indiscriminate use of drugs.
 

...

Md. Saiful Islam Siddiqui1*, Sharmin Akter2 and Mohd. Riajul Islam2 

...authorities must take deligent steps to control the uses of antibiotics in broiler industries, to stop the misuse of antibiotics, to ensure proper veterinary service and building mass awareness about ABR and its impact on public health.

...

Hafiz Muhammad Walayat Ali1, Hafiz Basheer Ahmad1*, Zaheer Ahmad Nazar2, Muhammad Akhlaq Mudassir1, Mahmood-Ul-Hasan1, Abdul Khaliq1 and Muhammad Shahzad Afzal1

...lysis showed that first eigenvalue was equal to (1.78) and represented that (35.6%) of the total variation. The 2nd eigenvalue (1.077) and represented of the total variation (57.2%) for the crop plant. For the ratoon crop, the first eigenvalue was (1.83) and presented (36.6%) of the total variation while 2nd eigenvalue (1.15) and represented (59.7%) of t...

Thekra Fadel Saleh, Omar Younis Altaey*

...dwide. Caecal microbial digestion is crucial for the growth and maintenance of the health in these birds. The main objective of this study is to understand the anatomy, histology, histochemistry, and distribution of lymphoid tissue in the caeca of Muscovy ducks. Samples from eight healthy adult Muscovy ducks (Cairina moschata) were fixed, processed, sectioned, and stained with Hematoxylin and Eosin as well as Periodic Acid Schiff: Alcian blue pH 2.5 and toluid...
Nasir Ali Tauqir1*, Asim Faraz2, Abdul Waheed2, Ayman Balla Mustafa3, Irfan Shahzad Sheikh4, Michela Pugliese5 and Shahid Nazir6
...y to determine nutrient digestion and nitrogen balance. Blood samples were collected through jugular vein two hours post feeding and were analysed for blood triglycerides concentration, total proteins and blood urea nitrogen according to standard procedures. Nutrient intake, nutrient digestion, nitrogen balance, milk production, milk fat, total solids in milk and solid not fat did not show any treatment effect. However, milk...

Khalil1*, Dwi Ananta1, Andri Bachtiar2, Hermon3

... four male Indonesian-indigenous Pesisir cattle in a 4x4 Latin Square design. There were four experimental diets: basal diet + fresh rice straw (FRS), basal diet + stacking-supplemented rice straw (SSRS), basal diet +rolling-supplemented rice straw (RSRS), and basal diet + wrapping-supplemented rice straw (WSRS). The diets were fed. Parameters measured included: live weight gain (LWG), dry matter intake (DMI), feed conversion ratio (FCR), and nutrient d

Imtiaz Kashani and Sher Khan Panhwar*

...xonomic variability. An eigenvalue 7.251 and canonical correlation 0.937 among four groups was appraised from PCI whereas Wilks’ Lambda test of function λ = 0.27 was significant at P <0.05. The highest variance percentage was the most correlated among the groups defined by total length, girth, 2nd dorsal fin, pectoral fin rays, eye dia, snout to extended gill cover and head length parameters. PC-II accounted for 13.9% of the variance that are m...

Amina Rahat1, Zahin Anjum1*, Sehlina Zehra2, Fatima Umal Baneen3 and Rabia Chishti1

... is also anticancerous. Nigela sativa is used for jaundice, paralysis, metabolic syndrome, insulin resistant syndrome, and high level of cholesterol, nerve tension problems; it stops hair fall and boost’s immune system. Based on the results of this study, it can be concluded that almost all the plant extracts showed potential antibacterial, insecticidal and antifungal activities against different. The research has focused on the potential therapeutic eff...

Monis Hussain Shah1*, Riaz Ur Rehman2, Riaz Ali Shah1, Farwa Batool3, Rizwan Rafique4, Muhammad Usman5, Sajida Bibi6, Sadia Yasin7 and Samida Qamar8

... at 5±2oC (In Refrigerator). The morphological attributes of plants were observed better in cv. Antarctica and Oxford. The research was repeated during the subsequent years. The variable response was observed in various varieties of tulip in Rawalpindi climate. The cv. Antarctica and Oxford were observed as best performer wise all the attributes. The same varieties are recommended to local farmers for cultivation as well. 

...

Skenndri Safae1*, Charrat Nadia2, Jmiai Mehdi3, Nassik Saadia1 

...n and the health of the digestive tract of the birds. The 18 farms were firstly categorized into three (3) groups according to their performances on a biosecurity level: P group for “poor” performances, D group for “decent” performances, and G group for “good” performances. Each farm was then sampled twice on day 11 and day 35 of the same production cycle. In order to evaluate the gut health of the broilers, we used a panel ...

Shujaullah Khan1, Zahid Hussain1*, Haroon Khan1 and Omer Suha Uslu2

... use of artificial intelligence (AI) can prove to be the most economical and environment friendly weed management strategy in wheat crop in the long run. In order to assess the impact of artificial intelligence (AI) on wheat crop in comparison with certain synthetic herbicides, a field experiment was designed in the Farm of the University of Agriculture Peshawar, in the wheat growing season of 2020-21. A Randomized Complete ...

Abdur Rauf1*, Farooq Jan1, Muhammad Qayash2, Zia-ul-Islam3, Rizwanullah1, Ikramullah Khan1, Muhammad Shuaib4, Muhammad Khalid5 and Samrin Gul6

... flavus and Aspergillus niger) were examined. The above-mentioned various ethanolic crude extracts of H. eichwaldii revealed the highest potential (15.82±0.40), (18.12±0.56), and (17.41±0.51) against S. aureus, a moderate activity (13.31±0.64), (15.43±0.52), (14.35±0.64), (11.34±0.50), (13.47±0.55), (14.21±0.67), (9.53±0.51), (15.32±0.53), and (2.15±0.65) against E. coli, B. su...
Umer Farooq1,2, Nimra Murtaza2, Abubakar Siddique1, Bilal Saleem1, Obaid Ur Rehman1, Nageen Zahra1, Muhammad Uzair1, Muhammad Naeem Riaz1,3* and Muhammad Ramzan Khan1*

Fuji Astuty Auza1*, Sri Purwanti2, Jasmal A. Syamsu2, Asmuddin Natsir2, Rusli Badaruddin1, Deki Zulkarnain1, La Ode Muh. Munadi1 

...s quality, and nutrient digestibility of village chicken. A total of 140 one-day-old chicks (DOC) were randomly assigned to one of five treatments in a completely randomized design, with four replicates per treatment. The treatments included P0 = 0% BSF larvae flour, P1 = 25% (3.74% BSF larvae flour), P2 = 50% (7.51% BSF larvae flour), P3 = 75% (11.26% BSF larvae flour), P4 = 100% (15% BSF larvae flour). The results showed that replacing fish flour with BSF la...

Dede Kardaya*, Deden Sudrajat, Dewi Wahyuni 

...(ADF) and hemicellulose digestibility (P<0.05), and increased feed efficiency and live weight gain of lambs (P<0.05). It is implicated that zeolite or urea-impregnated zeolite improves lamb performances probably as a result of the cation exchange capacity of zeolite or slow-release characteristics of urea-impregnated zeolite. 

...

Rizki Palupi*, Arfan Abrar, Fitri Nova Liya Lubis, Yuni Kurniati, Bianca Ikriza Octa Nadia Putri 

... in rations on nutrient digestibility, hematological status and malondialdehyde levels in broiler chicken blood. The study was conducted experimentally with a completely randomized design, which consisted of 5 treatments and 5 replications. The treatment was the use of a combination of cassava tuber and indigofera leaves meal (70%+30%; CCI) in the ration, namely: T0 (0% CCI in the ration), T1 (5% CCI in ration), T2 (10% CCI in ration), T3 (15% CCI in ration) ...

Abiyu Tadele 1,2*, Gebreyohannes Berhane2, Wondmeneh Esatu3

...west Ethiopia are the indigenous naked-neck and normal-feathered chicken genotypes, and the exotic Tetra H, a dual-purpose genotype that was imported from Hungary. Data were collected from 168 hens of the three genotypes kept under intensive management for 20 weeks. The Tetra H genotype had significantly greater (p < 0.05) body weight, feed consumption, hen-housed egg production, egg mass, and a lower (better) feed conversion ratio than the other genotypes....

Chitra Kumalasari1,2, Lovita Adriani2*, Indrawati Yudha Asmara3, Nazri Nayan4 

...ve tract, productivity, digestive system, and health of laying hens at an advanced age. This article attempts to provide a complete summary of the potential benefits of probiotics in prolonging the production cycle of late-phase laying hens based on existing literature. The research presented in this study offers pertinent insights and theoretical frameworks for enhancing productivity in aging hens, particularly for individuals engaged in small-scale farming.&...

Farha Deba1, Rubina Nelofer2 and Muhammad Irfan1*

...llus flexus FD6 and Alcaligenes sp. FD7 were verified by the molecular identification investigation using 16S rRNA sequence technology respectively. Out of these seven isolates, the Bacillus cereus FD2 strain produced the most keratinase (298U/mL), and was selected for more research. The Bacillus cereus showed the highest keratinase activity at neutral pH 7.0, with 1% inoculum size, 1% substrate and after 72h of the incubation period. This current study reveal...

Ayoub Hadjeb1,2*, Ismahane Lebbouz3 and Yasmine Adjami4

...f biomass conversion of digested food (ECD) and ingested food conversion efficiency (ICE) in Ghars and approximate digestibility (AD) in Mech Degla. The calculation of gravimetric index (nutritional) consumption and utilization of food showed a very highly significant difference (RRI,RGR, ECD and ICE: P <0.0001; AD: P <0.0004) between larvae fed by the dates of the three varieties of dates (Deglet Nour, Mech Dagla and ...

Rameez Raja Kaleri1,2*, Hubdar Ali Kaleri1, Nazeer Hussain Kalhoro5, Raza Ali Mangi3, Ghulam Mustafa Solangi4, Deepsh Kumar Bhuptani6, Sher Muhammad Khosa1, Abdul Wahid Solangi1, Ayaz Ali Lashari7 and Sheva Dari1

...c characterization of indigenous backyard poultry birds in Tando Allahyar. For this purpose, total 470 birds with 240 male and 230 females were brought under the study. Their phenotypic characteristics were collected thorough observing by the researchers from different UCs of district Tando Allahyar. The data regarding quantitative traits were observed using the measuring tap mentioned in the (FAO) manual. The findings of our study 86.69% out of the 470 birds ...

Amthal Ahmed Fouad1, Basem Mohamed Ahmed2, Momtaz Abdelhady Shahein1, Hussein Aly Hussein2* 

...pplied to detect RABV antigens in the brain of rabid dog. Relevant reads from the Ion S5 sequencing system were assembled into a draft genome. The draft genome was analyzed, re-assembled, and compared to representative RABV genomes from Africa and the Middle East region. Results: The complete genome of 5EG-QH19 strain extends for 11919 nt including the standard RABV protein genes in the correct order. Twenty-nine synonymous and five non-synonymous mutations we...

Elihasridas, Mardiati Zain*, Roni Pazla, Simel Sowmen, Qurrata Aini 

...ely high, so it has low digestibility. We evaluated the effectiveness of precursor rumen microbial growth supplementation to increase the digestibility of ammoniated aromatic lemongrass waste. The precursor rumen microbial growth supplement contains cassava leaves as a source of branched-chain amino acids, phosphorus, and zinc minerals, which was administered as follows: T0= ammoniated aromatic lemongrass waste, T1= T0 + 5% ...

Yingying Wang, Jianyi Yu and Lichen Zhou*

...nimals. The South China tiger, the most endangered tiger subspecies endemic to China, remain only a little more than 200 today, was susceptibly infected with Toxoplasma gondii. In this study, we used polymerase chain reaction to detect DNA, and enzyme-linked immunosorbent assay to test for T. gondii antibodies. Antibodies (S/P>0.31) to T. gondii were found in 20 (26.32%) of the 76 tiger...

Pham Tan Nha*, Le Thu Thuy

...eywords | Tre chickens, Digestibility, Lactobacilli, E. coli, Clostridium
...

Novirman Jamarun1, Roni Pazla1*, Arief2, Elihasridas1, Gusri Yanti3, Rani Winardi Wulan Sari4, Zaitul Ikhlas4

..., body weight gain, and digestibility of nutrients of goats fed mangrove leaves and T. diversifolia with different levels. The research employed 16 one-year-old goats and divided them into groups according to their body weight. The concentrate utilized in the study comprised tofu dregs, corn, rice bran, palm kernel cake, minerals, and salt. A randomized block design with four treatments and four groups of goat body weight as replicates was employed in the rese...
Hung Phuc Nguyen1*, Doan Van Thuoc2*, Nguyen Thi Trung Thu1, Huong Tran Thi Mai3, Nguyen Tran Khanh Hoa1, Nguyen Thi Tuyet Nhi1, Nguyen Phuong Thao1, Tran Thi Loan2 and Nguyen Thi Huyen My2
...s and nutrient apparent digestibility coefficients (ADCs) of red tilapia (Oreochromis sp.). FSR was obtained by fermenting SR with Bacillus subtilis V37. Five isonitrogenous and isoenergetic diets were formulated to replace 35% or 50% of FM by SR or FSR. The diets were denoted as follows: FMD, SR35D, FSR35D, SR50D, and FSR50D. The FMD (the reference diet) contained FM as a main source of dietary protein. A total of 300 fingerling red tilapia with an initial bo...

Sherein H. Mohamed1, Soad El Naggar2*, Ayman A. Hassan3, Mohamed A.M. Mousa3, Mohamed M. Basyony3, Mohamed F. Sadek3, Mohamed A. A. Ahmed4, Saadia M. Hashem5 

...productive performance, digestibility, cecal fermentation, antioxidant enzyme activities, immunity and meat quality. One hundred twenty six male growing New Zealand White rabbits aged eight weeks old with an average body weight of 675±47.95 g were divided into six equal groups 21 rabbits each, control diet group, C fed the basal diet and the other five groups (GE, ClE, ThE, TE, and CE) fed on basal diet supplemented with 200 ppm of garlic, clove, thyme,...

Ali A. M. Al-Kuhla1*, Yaman S. Fadhil1, Saffanah K. Mahmood2 

...ould be more helpful in digestion and absorption of nutrients in the small intestine. 

...

Jameel-ur-Rehman1, Asghar Ali Kamboh1*, Ahmad Ali Moryani1, Riaz Ahmed Leghari1, Abdullah Sethar2, Ali Raza Nizamani3, Abdul Ahad Soomro3, Kanwar Kumar Malhi1 

.... (78.3% & 58.3%), Shigella flexneri (61.6% & 53.3%), Pseudomonas flavescens (55% & 53.3%), Pseudomonas aeruginosa (40% & 35%), Staphylococcus aureus (33.3% & 25%), Streptococcus agalactiae (21.6% & 18.3%), Klebsiella pneumoniae (15% & 13.3%), Staphylococcus epidermidis (13.3% & 10%), Klebsiella oxytoca (10% & 8.3%), and Streptococcus pyogenes (6.6% & 1.6%) in market and pond fish samples respectively. According to ant...

Sameh Abdel-Moez Ahmed Amer1*, Aly Mohammed Ghetas1, Asmaa Mahmoud Maatouq1, Hagar Magdy Ahmed1, Khaled Mohamed El-Bayoumi1, Mohamed Abd El-Rahman Bosila1, Ahmed Ali El-Shemy2

Eman M. Abdelhady1, Nader Y. Mostafa1, Mohammed A. El-Magd2* and Ghada A. Kirrella1

...sp;  during its refrigerated storage for 9 days. The size of CNPs ranged from 150 to 350 nm as determined by transmission electron microscopy. Comparing the effect of the two chitosan products, CNPs exhibited higher antibacterial properties against S. aureus and E. coli, as revealed by lower minimal inhibition concentrations than the CP. Among these bacteria, E. coli was the most sensitive to CP and CNPs. Minced meat samples treated with CNPs showed signi...

Xiang Li1, Zhijun Zhao1, Lei Zhang2, Jinhuan He1, Fengling Li1, Jing Li1 and Chunmei Pan1*

... slaughter performance, digestion and metabolism of nutrients and economic benefits of piglets, this paper analyzes the effect of fermented feed (taking grape pomace residue as example) on the nutrient apparent digestibility of piglets. Through the determination of tannin content in grape pomace residue, the nutritional value of grape pomace residue was evaluated, and the influence of feed with different grape pomace residue...

Abdullah Naser1*, Nirwana1, Sri Wulan1, Zaenal1, Mustafa1, Effendy2 

... growth performance and digestibility of sheep reared in stalls with either palm leaf roofs or galvanized iron roofs. This study used a 2 x 5 split plot pattern randomized block design and was replicated three times. Livestock was grouped based on body weight. The main plots are two types of stable roofs (palm leaf roof and galvanized iron roof). The subplots were five levels of soybean meal being substituted with fermented kapok seeds, namely: R1: 100% Soybea...

Nwafili Sylvanus Anene, Ibinabo Jessica

...uaculture production in Nigeria.


 

...

Yuyu Wang1,2, Mingzhu Li 3,4, Gang Lin4, Xiaohua Guo5, Aihua Sun5, Hao Dong5, Qinghui Ai1,* and Kangsen Mai1,*

...hytrium meal on growth, digestive enzymes activities and fatty acid composition of turbot (Scophthalmus maximus L.) larvae. Four isonitrogenous and isolipidic diets were formulated to contain 0 (S0, control diet), 50 (S5), 100 (S10) and 150 (S15) g kg−1 Schizochytrium meal. Fish (initial body weight, 0.06 g) were randomly allotted to 12 square fiberglass tanks. Fish were fed 5 times daily (7:00, 10:00, 14:00, 17:00 and 21:00) for 28 days. No significant ...

Samia Afzal1*, Jahanzaib Ahmad1, Iram Amin1, Liaqat Ali2, Muhammad Shahid1, Mohsin Ahmad Khan1 and  Muhammad Idrees1

...n hepatitis B surface antigen (HBsAg) for viral development and release from hepatocytes. It causes the most severe viral hepatitis and suppresses the serological and molecular markers of HBV infections; for example, Hepatitis B envelope antigen (HBeAg), inhibition and reduction of HBV DNA titer during HDV infections. Very few studies described the clampdown of HBV-related markers during HDV replication and the current study...

Stepanus Pakage*, Daud Krey, Trisiwi. Wahyu Widayati, Deny Anjelus Iyai, Agustinus Gatot Murwanto, Alnita Baaka, Daniel Yohanes Seseray, Djonly Woran 

...conomic efficiency of Indigenous Papuan pig farmers in Manokwari Regency. The method used is the Ordinary Least Square (OLS) and Maximum Likelihood Estimation (MLE) methods on the Cobb-Douglas production function model and the Technical Efficiency Effect Model option. The results showed that piglets, feed, and firewood had an effect on pig production. Pig farmers in Manokwari Regency have been allocatively efficient, but technically and economically inefficien...

Bdour Muhammed Al-Shweily1*, Jawad Bulbul Al-Zaidawi2, Muhammed Jubair Hanawi1 

... integration of these indigenous biological control agents yielded notable mortality rates for the targeted larvae and pupae. The highest rates were achieved when using a higher concentration of fungi, specifically 20×10^6 spore/mL, in combination with 100 infective juveniles/mL of nematodes (resulting in an 83.33% mortality rate for larvae and 80% mortality rate for pupae). Remarkably, the mortality rate of the treated larvae surpassed that of the treat...

Meryem Betmezoğlu1*, Dilek Arsoy2 and Mahmut Çerkez Ergören3

.... Goat production and indigenous goat breeds (Cyprus Native hair Goat, Damascus) are important for the Cyprus’s agricultural diversity Therefore, the allele frequencies of Caprine Prnp gene variants should be well understood for goat breeding programs in Northern Cyprus. This current study was designed to determine the putative allele frequencies and genotype distributions of Caprine­ Prnp gene variations in Northern Cyprus Damascus, Cyprus Native Ha...

Omar Berkani1*, Souheila Slimani2, Nora Sakhraoui3 and Cherif Abdennour1

...ction of domestic males pigeons (Columba livia domestica) subjected to a long photoperiod (19L: 05D). Therefore, thirty pigeons were divided equally into six groups; group C used as a control, group PO used as a positive control that treated by PO (300 mg/k b.w/day), CYP1 and CYP2 groups were respectively treated by 10 and 20 mg/Kg b.w/day, in addition to CYP1+PO and CYP2+PO groups. All treatments were realized by gavage for...

Sehrish Ishtiaq1, Mahroze Fatima1*, Syed Zakir Hussain Shah2, Noor Khan3, Muhammad Bilal4, Maryam2 and Sobia Nisa1

...y composition, nutrient digestibility and mineralization of silver carp, Hypophthalmichthys molitrix (average initial weight 13.7 ± 0.05 g). Nine isonitrogenous, isocaloric and isolipidic diets with three Ca levels (0, 1 and 2%) combined with three P levels (0, 1 and 2% P) were prepared. Results showed that silver carp fed the diet supplemented with 1% Ca and 1% P level yielded the similar (p > 0.05) growth performance as was recorded for that fed di...

Olabode, H.O.K1, Mailafia, S1, Martha Echioda-Ogbole1*, Ameh, J.A1, Amadi, K.I1 and Meseko C.A

...Municipal Area Council, Nigeria. One hundred (100) blood samples collected randomly post slaughter from chickens in live bird markets were screened and characterized using Agar Gel Immuno Diffusion and Haemagglutination Inhibition Tests at the Avian Influenza laboratory, of the National Veterinary Research Institute, Vom Plateau State. A structured Questionnaire was also administered to 50 poultry farmers. Results revealed an overall sero-prevalence of 5% with...

Blessing Ndenum Peter1, Olufemi Bolarinwa Adedeji1, Reuben Chukwuka Okocha2,3*, Ekemini Moses Okon4 

...t frequently checked in Nigeria, and consumers are unaware of the risk of ready-to-eat (RTE) shrimps purchased in Ibadan seafood markets. Therefore, this study determined the microbial quality of ready-to-eat shrimps from three major seafood markets in Ibadan. Ready-to-eat shrimps were collected from 80 outlets at the three major seafood markets in Ibadan (50 from Bodija, 15 from Alesinloye and 15 from Eleyele) and checked for microbial presence, microbial cou...

Yunilas1*, Muheri Indra Aja Nasution2, Edhy Mirwandhono1, Adi Fathul Qohar3 

...astric livestock cannot digest it. Chitin is found in the BSF exoskeleton which is bound to proteins, minerals, and pigments, so it is necessary to do processing to reduce the chitin content first by fermentation using organic acids. Therefore, this study aimed to determine the effect of using organic acids (propionic acid and formic acid) and various fermentation times in reducing the BSFP chitin content. Completely Randomized Design (CRD), factorial 3 x 3 wi...

H.A. El-Nagar1, A.M. El-Hais2, M.S. Mandouh2, W.M. Wafa1*, A.H. ABD El-Aziz, K.A. Attia4 

...P), black seed oil (BS, Nigella sativa), or their combination on growth performance, immunity, and health status of females of newborn Friesian calves. A total of 20 newborn calves (37.51±0.92 kg LBW) were divided into 4 groups. During the suckling period, animals in the 1st group were fed a starter without any addition (G1, control). Animals in G2, G3, and G4 were fed the same starter supplemented with BP (10g/kg), BS oil (10g/kg), or their combination...

Wei Fang1,2,3, Jiawei Hong1,2,3,4, Zhengyi Fu1,2,3, Jing Hu1,2,3, Gang Yu1,2,3, Zhenhua Ma1,2,3* and Humin Zong5*

...red by TGICL to obtain Unigene, and bioinformatics methods were then adopted to perform functional annotation classification, metabolic pathways and SSR feature analysis. The results showed that a total of 42.40 Gb data were obtained from transcriptome sequencing, and 38,817 Unigenes were obtained after assembly and de-redundancy; Using BLAST to annotate the assembled unigenes with seven f...

Bilal Ahmed Shah1, Muhammad Avais1*, Jawaria Ali Khan1, Masood Rabbani2, Aftab Ahmad Anjum2, Muhammad Asad Ali2, Muhammad Awais1, Sohail Ahmad3 and Shahan Azeem2*

...fter confirmation for antigenicity and immunogenicity and evaluated for safety, side effects, challenge protection assay, sterility and humoral response in laboratory animals. Safety test showed no general adverse reactions to the vaccines when injected either into mice or rabbits or cow calves. Challenge protection studies revealed a significantly higher survival rate in vaccinated mice and rabbits compared to placebo groups. None of the vaccines when streake...

Swagata Das Gupta1, Majharul Islam1, Towhida Kamal2,Md. Rayhan Faruque3,Md. Shohel Al Faruk4* 

...the esophagus and upper digestive tract in pigeons.A common case of pigeon cankers was diagnosed in two pigeons 40 days of age who came to S.A. Quaderi Teaching Veterinary Hospital, Chattogram Veterinary and Animal Sciences University (CVASU).Clinical history indicated that the birds were taking both food and water from the same source, clinica...

Ahmed Jaafar Mousa*, Nothaila Rasheed Hamid 

...otozoan widely found in pigeons and other wild birds, causes the parasitic disease known as “avian trichomoniasis”. The complications of this illness include lesions in the upper gastrointestinal tract. The present study’s goal would be to study the prevalence and genetic characteristics of T. gallinae in racing pigeons in Thi-Qar province, Iraq, utilizing the ITS1-5.8s rRNA-ITS2 gene. 100 samples were coll...

Shaker B. Al Suwaiegh 

...giant reed on nutrients digestibility and growth performances and serum biochemistry of goats in arid subtropics. Twenty-four goats average 29.70 ± 1.17 kg body weight (BW) were randomly distributed over four groups. The goats were fed four diets composed of 50.0% concentrates and 50.0% forages. The four diets formulated according to replacement of alfalfa hay with giant reed were control alfalfa hay diet (T1; 50.0% alfalfa hay) and giant reed rations (...

Abdul Latif Rind1*, Shahid Hussain Abro1, Rameez Raja Kaleri2,3, Ghulam Mustafa Solangi4, Raza Ali Mangi5, Muhammad Anees Memon6, Depeesh Kumar Bhuptani7, Sana Noor8, Zainab Lanjar9, Zahid Ali Mangrio10 and Abdul Wahid Solangi

...sting to identify PPR antigens. The analysis of PPR prevalence was carried out according to breed, sex, age, and Taluka. The overall PPR prevalence was found to be 20%, with the highest prevalence rate (50%) observed in Jam Nawaz Ali Taluka and the lowest (7.5%) in Shahdadpur Taluka. The prevalence was higher in goats (30%) than in sheep (10%). Female animals had a higher prevalence than males, with a prevalence of 25% in female goats and 7% in female sheep. T...

Shakirat Bolatito Ibrahim1, Raheem Olatunji Aminu1,2*, Aisha Olushola Arowolo1 and Adams Sanusi Musa1

...useholds in Ogun State, Nigeria. Data were collected cross-sectionally from 208 households containing 1546 individuals. The results show that 73% of farmers owned the cultivated land, while 26% had rights over the cultivated land. The average distance of the sampled households to public and private hospitals in the study area is 2.33km and 0.88km. Most households consume cereals, roots, and tubers. As indicated by the household dietary diversity score, 85.64% ...

Romedi Çelik

...cteristics of the angut pigeon breed, which is native to Şanlıurfa, where pigeon breeding has been done traditionally for a long time and is also one of the domestication centers. In this study, we adopted 146 angut pigeons comprising 72 males and 74 females. Additionally, four different colour variations were determined: yellow angut (41.1%, n = 60), red angut (35.6%, n = 52), white ang...
Ummul Firmani1,3, Rahmi Nurdiani2, Arning Wilujeng Ekawati2 and Happy Nursyam2*
...ntify bacteria from the digestive tract of milkfish (Chanos chanos Forskal) and investigate their potential as probiotic candidates. Bacteria were isolated from the digestive tract of milkfish and identified using PCR technique and 16S rRNA gene sequencing. The probiotic potency was determined using amylolytic assay, synergistic activity, hemolytic activity, and antagonistic activity. The results showed that there were sever...

Sidra Mumtaz1, Sidra Arshad1, Rizwan Ullah Khan1, Muhammad Usman Rashid2 and Naila Malkani1*

...xpression in breast tumorigenesis. A thorough literature search was performed and 73 relevant studies were identified which were further scrutinized and finally 26 studies covering 4553 subjects were included for analysis. A random effect model was applied and correlation was calculated using odds ratios (OR) at 95% confidence intervals (CI). Combined OR calculated showed a significant relation between Ets factor expression and breast cancer risk (OR=3.185, 95...

Lu Liu1, Xiang Zhao2, Yue Qin2, Tianxiang Gao3 and Tianyan Yang3*

...4413) named Lophiomus setigerus, indicating that the latter might be a misidentification. Phylogenetic analysis based on PCGs suggested that genus Lophius was more closely related to Lophiodes than Antennatus.

...

Wafa Akram1, Shahan Azeem2, Shafqat Shabir1, Haroon Akbar1*, Warda Gill3 and Muhammad Imran Rashid1*

... diagnosed in a captive tiger through microscopy and L. infantum was confirmed by PCR and sequencing analysis. DNA sequencing of the amplicon revealed close homology with Leishmania sequences available in GenBank. Alignments and phylogenetic analyses of the Leishmania infantum from a tiger in Pakistan indicated 94-100 % identity with Leishmania from animals and 98.8-100% with Leishmania from humans origins suggesting the nee...

Waseef Ullah Khan*, Yasser Durrani and Muhammad Ayub

...ial preservative for refrigerated mango OLE blend.

...

Slamet Widodo1*, Mohammad Ikhsan Shiddieqy1, Teguh Wahyono2, Yeni Widiawati1, Zultinur Muttaqin1

...tween nutrient content, digestibility, and gas production of several forages in Indonesia that could help to develop prediction equations about feed digestibility and gas production to these forages. Parameters measured in this study were crude protein (CP), neutral detergent fiber (NDF), acid detergent fiber (ADF), lignin, fat, minerals, dry matter digestibility with pepsine (DMdPeps), or...

Erkinjon B. Shakarboev1*, Abat S. Berdibaev2

...ovis Cobbold, 1869, Hydatigera krepkogorski Schulz et Landa, 1934 and Multiceps skrjabini Popov, 1937, and 7 species from the class Nematoda – Capillaria putorii Rudolphi, 1819, Uncinaria stenocephala Railliet, 1854, Crenosoma vulpis Rudolphi, 1819, Oxynema numidica Seurat, 1915, Cylicospirura subaequalis Molin, 1860, Pneumospirura capsulata Gerichter, 1948 and genus Dipetalonema sp., were for the first time identified in the fauna of Karakalpakstan.&nbs...

Arief1*, Roni Pazla2

... in the consumption and digestibility of crude fiber, crude fat, and nitrogen-free extract. A mixture of forages, including Mirasolia diversifolia, Indigofera zoolingeriana, and Gliricidia sepium, combined with palm concentrate, can balance the company’s ration FCM milk production.
 
Keywords | Gliricidia sepium, Goat, Indigofera zoolingeriana, Milk, Mirasolia diversifolia, Palm concentrate
...

Nguyen Thi Thu Hien1, Dong Huu Rin2, Nguyen Xuan Hoa1, Le Viet Tuan Khanh2, Phung Thang Long1*, Dinh Thi Bich Lan1

...E3), and evaluate its antigenicity as a base for further studies to against M. hyopneumoniae. The gene encoding M. hyopneumoniae P36 protein isolated from DNA of fresh lung tissue samples from PEP infected pigs was cloned into pGEM®-T Easy vector for sequencing, and then was inserted into pET28a vector to express the protein. The cloning result revealed that the gene encoding the M. hyopneumoniae P36 protein was 432 bp in length, and 99.07% similarity to t...

Umar Salisu Ahmad1, Adamu Abdul Abubakar1,2*, Hassan Abubakar Bodinga1, Nura Abubakar1, Ekaete Ime Oviawe1, Zaid Shehu3, Abubakar Musa Mayaki3

... materials, and other indigestible materials mixed with ruminal ingesta were found in the rumen. In conclusion, careful examination of rumen impaction should be considered now in calve 6-month below when presented with gastrointestinal abnormalities in developing countries. With accurate radiographic diagnostic investigation, the lives of the affected calves can be save. 
 
Keywords | Rumen impaction, Calves, Sokoto Gudali and...

Lawrence Olusola Oparinde1*, Adewale Isaac Olutumise2,3 and Ademola Adegoroye

...p farmers in Southwest, Nigeria. A multistage selection process was employed to pick 450 arable crop farmers. The collected data were analyzed with the use of descriptive statistics, probit regression model and Gini coefficient analysis. Findings from this study revealed that the adoption of agroforestry technology increased income inequality among male and female crop farmers. Also, the difference in the income inequality level between adopters and non-adopte...

Salma Sharif1, Rana Arsalan Javaid2*, Abid Majeed2, Muhammad Shahzad Ahmed2, Qurat ul Ain Sani2, Faiza Siddique2, Muhammad Arshad3 and Niaz Ali

...carried out to assess indigenous rice germplasm using qualitative and quantitative features, as well as to select attractive genotypes for use in breeding programs in the future. A set of 65 accessions were evaluated in the field of Crop Sciences Institute, NARC following factorial design with three replications. highly significant differences were observed for all the quantitative traits as days to heading, days to maturity, plant height, number of tillers, p...

Anshara Javed Qureshi and Ishrat Aziz*

...pillariasis in domestic pigeons (Columbia Livia) in the Bahawalpur area of Pakistan was investigated in May 2023. Fecal samples of 100 pigeons (30 males and 70 females) belonging to 30 different breeds were collected from four different houses of the Dilawar colony area of Bahawalpur, and proceeded qualitatively through direct microscopy and floatation method. In this study, 18 (11 males and 7 females) out of the 100 samples...

Retno Setyaningsih1, Sri Murtini2*, I Wayan Teguh Wibawan2, Surachmi Setiyaningsih2, Ekowati Handharyani3

... the presence of RHDV antigen in asymptomatic seroconverted RHDV rabbits in West Bandung District, West Java Province, Indonesia also to see the relevance of seroconversion within rabbit’s profile. Liver, intestines, and faeces were collected purposively from seroconverted rabbits which showed no clinical signs of RHDV. Samples assayed with commercial ELISA Kit using DAS Technique. The informations (age, sex, breed) of antibody titers against RHDV from p...

Qi Jiayi1, Huang XuMiao2, Lin Wei3, Ao YaHui3, Zhao Wenting2* and Cao Chunhao3*

...n essential role in tumorigenesis and progression, and sought to develop an immune-related lncRNA signature to assess the value of immune-related lncRNAs to discover their prognostic values. RNA-seq and clinical information were obtained from the Cancer Genome Atlas (TCGA) database, which aims to screen for immune-associated lncRNAs based on constructing a risk scoring formula. Besides, in virtue of univariate and multivariate Cox regression, immune-associated...

Hafiza Mehwish Iqbal1, Salman Khurshid1*, Saqib Arif1, Qurrat-ul-Ain Akbar1, Saba Iqbal2, Shahid Yousaf3, Kainat Qureshi1, Abdul Karim Khan5, Abdul Ahad6, Aqeel Ahmed Siddique4 and Neelofar Hamid7

...fungi viz., Aspergillus niger, Alternaria alternate, Fusarium solani and Aspergillus flavus were studied by Well diffusion method. Three solvent extracts Aqueous, Acetone and Methanol with concentrations (5,10,15 and 20%) was prepared. Our study showed that maximum zone of inhibition (ZOI) was observed against bacteria for M. Koenigii i.e., 22mm and 23 mm for fungi, while; O. basilicum and S. asoca plants have21 and 22mm MIZD. Methanol 20%has maximum antioxida...

Shamsher Ali* and Naheed Baloch

...essential oils of two indigenous plant species i.e., colocynth, Citrulus colocynthis, and Akk, Calotropis gigantea by using different 03 and 06ml/L concentrations of each plant seed oil against the lesser grain borer, Rhyzopertha dominica, and Khapra beetle, Trogoderma granarium infesting wheat grains. Observations were made on mortality, weight loss, and insect damaged grain. The results show the maximum mortality 62.0% of R. dominica and 34.3% of T. granariu...

Nguyen Huu Van1*, Nguyen Thi Mui1, Dinh Van Dung1, Van Ngoc Phong1, Tran Ngoc Long1, Le Tran Hoan1, Le Duc Thao1, Vo Thi Minh Tam1, Ngo Mau Dung1, Bui Van Loi1, Nguyen Xuan Ba1, Ton Nu Minh Thi2, Nishino Naoki2

...in the diet on nutrient digestibility, rumen volatile fatty acid (VFA) profile, growth and carcass performance of Phan Rang sheep raised in Ninh Thuan province, Vietnam. Twenty-five intact male Phan Rang sheep averaging body weight of 15.4 ± 1.3 (SD) kg/animal were randomly assigned to five groups with different levels of concentrate, including: control (C) (ad libitum access to grass feeding); 0.75% (control plus 0.75% of concentrate); 1.5% (control pl...

Abida Mushtaque1, Ali Ahmad Sheikh1, Aamir Ghafoor1*, Wasim Shehzad2 and Nadeem Ahmad3

...onfirmed by restriction digestion. The rOmpH protein was purified by immobilized metal affinity chromatography by using His-Ni resins to get desired protein and characterized qualitatively by SDS-PAGE by using His antibodies and western blotting. Once purified the immunogenicity of rOmpH was evaluated in BALB/c mice. The expression of recombinant OmpH protein (rOmpH) resulted in its accumulation in cytosol. The purified rOmpH protein had a molecular weight of ...

Shehar Bano Bhatti1, Muhammad Irfan Malik2, Rabia Faryad Khan1, Mahmood Ul Hassan2, Muhammad Shahbaz Aslam3 and Zaigham Abbas1*

Iko-Ojo Charity Ikwe Agada, James Agbo Ameh, Olatunde H. Olabode and Martha Echioda-Ogbole*
...g the SNAP parvovirus antigen detection kit (IDEXX Laboratories, United State). Other disease conditions include helminthiasis 25 (12.7%), canine babesiosis 3 (1.5%), while 37 (18.78%) cases were recorded as canine distemper and other bacterial enteric diseases. Monthly distribution of CPE cases in this study showed significant difference (p<0.05) characterized by high prevalence in the dry season with peak of infections in the month of January. Breed dispo...
Moustafa A. Zaghloul1*, Mohamed F. Azooz1, Saleh E. Ali1*, Heba M. Soliman1, Maha M. Sayed1, Mohamed H. Kafafy2 and Alaa R. Morsy1
... to use artificial intelligence to predict the immunogenic landscape of circulating lumpy skin disease in the Egyptian cattle dairy sector, which will lead to universal blueprints for multiepitope lumpy skin disease vaccine designs. A total of 40 skin nodule samples were collected from clinically affected cattle to detect LSDV using PCR targeting GPCR, RPO30, P32 and EEV glycoprotein genes. The amplified products of samples detected in the skin nodules of catt...

Abdul Aziz1,2, Hamad Bin Rashid1*, Muhammad Arif Khan1, Asim Khalid Mahmood1, Ayesha Hassan1, Hamid Akbar1, Sadaf Imran1, Naveed Hussain1, Muhammad Umar1, Muhammad Asif1, Sajjad Javaid1 and Mamoona Chaudhry3

...d fat was enzymatically digested with 0.1% collagenase-I. It was then centrifuged and the SVF pellet was collected. The pellet was characterized through total viable cell count, cell per gram and percentage of viability by using Neubauer’s chamber. The average cell yield per microliter was 2.992±1.527 x 104 cells/µl. SVF per gram of adipose tissues was 2.992±1.527 x 106 cells/g, percentage of viability was 97.98± 0.31%, while n...
Rabiea Pervaiz, Shaista Bano*, Sarfraz Ali Tunio, Abdul Nabi Jatt and Aisha Amber Soomro
...izosphere soil of the indigenous Mangifera indica (mango) plant. Identification of the isolated strains was determined by sequencing their 16S rRNA genes. The isolates identified as B. pseudomycoides were screened for their antibacterial potential. In order to circumvent the trouble created from rhizoidal colony morphology of B. pseudomycoides, a colony mutant of a strain showing strong antibacterial activity was obtained by screening/selection method and desi...
Muhammad Fiaz Qamar1*, Tahir Hussain1, Iram Liaqat2, Madiha Kiran1, Abdur Rahman Ansari3, Faiza Yasmeen1 and Tahira Batool3
...; line-height: normal;">Pigeons (Columba livia), the members of Columbidae family are domesticated all around the globe in the villages, towns and cities. Jhang is a distant and underdeveloped district of Punjab province, Pakistan. The purpose of the present study was the determination of the prevalence of gut parasites in domestic pigeons through routine laboratory methods in district Jhang. Fresh fecal sample...
Kanwal Nisa1, Sadia Roshan1, Shazia Shamas1,2*, Raheela Atta Mustafa3, Shamaila Irum1, Kalsoom Sughra4 and Memoona Iqbal1 
...ker Prostate-specific antigen (PSA). Prevalence was recorded in Gujrat, Punjab, Pakistan. Blood samples of normal and prostate cancer males were taken from Gujrat, Punjab, Pakistan. Information including age, marital status, smoking, Prostate-specific antigen (PSA), testosterone, and any previous record was obtained with the patient’s consent. Exclusion and inclusion criteria were defined. The samples of control and pa...

Roheela Yasmeen1,3* , Faheem Hafeez1 , Umme Ammara1 , Rubab Younas1 , Sibtain Ahmad2 , Zulfiqar Ali3, Zaheer Ahmad Nasir4 

...collected samples were digested with aqua regia and analyzed by Inductively Coupled Plasma Atomic Mass Spectroscopy (ICP-MS). Fourteen metals were detected and categorized as essential (Calcium (Ca), Potassium (K), Sodium (Na), Phosphorus (P), Magnesium (Mg)), trace (Zinc (Zn), Copper (Cu), Iron (Fe), Manganese (Mn)) and heavy metals (Cadmium (Cd), Chromium (Cr), Mercury (Hg), Nickel (Ni), Lead (Pb)). In general, the concentration of all essential, trace, and...

Rahmat Elahi1, Gulnaz Parveen1*, Nazara1, Faryal Ali2, Nain Tara3 and Saba Iqbal1

...d seeds and Aspergillus niger accounted for 13.32% significantly was less compared to untreated seed. It appears that Fusarium semitectum was found in 13.33% of untreated seeds, while Alternaria solani accounted for 6.66%. However, in the treated varieties, both of these fungi did not appear. Aspergillus niger, on the other hand, had the highest colonies in both treated (46.64%) and untreated (56.66%) seeds. Additionally, Fu...

 Shawki, Khaled K.; Carter, M.J.; Alnashar, Nariman M., El-Farrashl, Mohamed A. and Taher, Sahar

.... Noroviruses form an antigenically diverse group of agents and cross-reactive immunity is poor. These features present a considerable challenge for vaccine development. Current approaches have been based on the use of empty virus-like particles produced by self-assembly of the virus capsid protein. In this study we employed a new approach for engineering stable particles of Hawaii virus capsid protein by deleting those immunodominant regions (P2subdomains) th...

Hemeida, A. A., Osman, M., El-Shahat, Mohamed, Hashem, Medhat H., Mahmoud, Amal and Dahi, Hosni

...f Center of Cardiac and Digestive System, Sohag, Egypt aged from 20 to 55 years. Then, confirmed by Reverse Transcription Polymerase Chain Reaction (RT-PCR) for HCV. The confirmed patients were received 12 vials of BEG FN- 2a for 12 weeks (1801U/ml weekly) plus ribavirin (1000 mg for 75 Kg or 1200 mg for > 75Kg- Roche) and follow up by RT-PCR. The results showed that 67 patients (72.8%) responded to the treatment (group A), while 25 patients (27.2%) non res...

Elkalamawyl, I.M.; Elhddadl, S.; Swelim2, M.A.; Hamdy2, S.M. and Fahmy3, Hanan A.•

...titis B virus Surface antigen (HBsAg) were characterized. The mutants were isolated from asymptomatic patients who were found to be persistently positive for both I-BsAg and anti-hepatitis B surface antibody (anti-HBs) and a long-lasting anti-HBc (core) IgM. Polymerase chain reaction (PCR) and sequencing were performed to surface antigen region and revealed two genotype D mutants 1--1BV. Aligned with known I-BsAg sequences f...

Raof*, Amal M. A.; Haleem*, Iman Y.; Aly*, Nawal M.; * *Garhy, M.M. and Hosny***, Gehan A.

...of FMD virus by using antigen detection ELISA. The saliva and vesicular lesions of the diseased animals were serotyped as FMD serotype O.

...

Omarl , Dalia M.; El-Ibiaryl , Elham A.; Sadik2, Atef S.; Abdel-Ghaffar2, Mamdouh. H. and Othman2, Badawy A.

...entified and subtyped antigenically by serological tests using Haemagglutination inhibition test (HI) using standard Al antisera and genetically by RT-PCR using specific primer. All the isolates were confirmed to be H5N1 and grouped according to the year of isolation as 2006, 2007, 2008, 2009 and 2010 group isolates. The HA titers of the above mentioned isolates were 6, 7, 8, 7 & 7 respectively. All the isolated Al viruses were titrated in ECE and examined...

Zaghloull , A.H.; Mahmoud2, Amal; Hassanl , H.Y.; Hemeida2, A.A.; Nayell , M. A. and Zaghawal , A.A.

...ed and labeled with digoxigenin and used as diagnostic probe for BEF virus infection using dot-blot hybridization. Thirty six samples were collected from Menoufia Governorate (Berket El-Sabaa, Tokh Tambesha and Salamon regions) and Demiate Governorate and were tested in dot-blot hybridization. BEFV was detected in 24 out of 36 samples (66.7%) as follow: 9/9 (100%) correspond to Berket El-Sabaa and Tokh Tambesha regions and 3/9 (33.3%) correspond to Demiate and...

Mansour, Abeer E.M.; Khodeir, M.H. and Hussein, A.M.H.•

...and complement fixing antigen revealed that CaC12 and MSG induced higher values than that obtained with the blank control batch where Ca C12 potentially facilitate the adsorption and penetration of FMDV to BHK cells increasing the virus attachment to the cell receptors. On the other side, MSG enhanced cell 1 division due to its of amino acid content and accordingly increased the available cell number for virus replication. There will be a need to more research...

Abo Elmagd l , Enas K.; Abdel-Wahab l , Kouka S.; Alrasheedy l , Zeinab E. and Khalifa2, Ahmed S.

...s (HCV) including HCV antigen detection in peripheral blood mononuclear cell (PBMNC) lysates, antibodies to HCV core, NS3, NS4 and NS5 epitopes by enzyme linked immunoassay (ELISA) in mothers and their infants using serum samples for both mothers and infants plus saliva for infants, in addition to detection of serum HCV- RNA (viremia) by reverse transcription polymerase chain reaction (RT-PCR). Also detection of antiHIV IgG class antibodies in mothers' sera. T...

Abo-Elmagdl , Enas K;El-Mougy2, Hala M. T. and Abd El-Salam 3, Manal

... for detection of RSV antigen in nasal wash specimens collected from Egyptian infants and young children with the detection of RSV-RNA by reverse transcription polymerase chain reaction (RT-PCR). Furthermore, to study the frequency of RSV infection in Egyptian infants and young children during the winter season 2009/2010 and the effect of certain risk factors including age and gender on the extent and impact of RSV infection. The study included 72 infants and ...

SHARAW11'2*S S. S. A.; AL-HOFUFY2, A. N. and AL-BLOW12, M. H.

...erved while CPV virus antigen was detected and identified by indirect immune-fluorescent assay (IFAT) in Vero cells. A trial for development of a simple and rapid qualitative Real-time polymerase chain reaction (RT-PCR) was applied using primer site belongs to Capripowirus to detect CPV load in prepared tissue samples comparing to inoculated

...

El.-Kadyl, M.A.S; Badr2, A.B.; zein3, Saiwa N. and Khalifa4, M.A.A.

...000, respectively.The antigen dilution end point was 1/800. Ribavirin had a higher inhibitory effect (80%) when sprayed at lower concentration (the minimum inhibiting concentration is 0.0001%) before one hr of inoculation. Salicylic acid and Parahydroxy benzoic acid has the same effect (80% reduction) at concentration of 0.01% and 0.1, respectively in the treatments inoculated one hr. after sprays. Six different chemical compounds applied as foliar sprays with...

Ahmed*, Amal A.; Khatab*, Eman A.H. ; Dawood*, Rehab A. and Ismeil*, Amira .M.

... for tip culture . Murashige and Skoog medium (MS ) supplemented with (0.2 mg/L BA ) and (Img/l BA and 0.5 mg/L kinetin ) were used for proliferation and micropropagation of the infected shoot clumps respectively. The plantlets were rooted in MS medium supplemented with (1.5 mg/l NAA ) . Plantlets regenerated from meristem measuring 0.1 mm and 0.2 mm yielded (90 and 80 % ) for CLV and (92 and 80 ) for CarVMV virus-free tested plantlets respectively,while large...

Hussein, HA; sultan, HA. Al Deeb AH and AA. El-Sanousi

...using heterologous H5 antigen which represent non of the vaccine antigens. The samples were collected from 578 houses mostly commercial layers. H5Nl viral nucleic acid was also detected in swab samples collected from mortalities occurred in some of vaccinated birds after vaccination policy has been applied in Egypt at March 2006. The viral RNAs of the detected H5Nl circulating viruses between Feb 2006 and Feb 2008 in Sharkia...

Madbouly, H.M; Orkhan, M.H**; Nagwa El-khoty

...ngolia, Iran, Korea and Nigeria and differed from those strains isolated in South-Eastern of Asia which contains Cambodia, Thailand, and Viet Nam Al H5Nl strains. The phylogenetic analysis of HA, NA, and Matrix (M) genes of Egyptian isolates of January 2007 from the same locality showed minor antigenic variation from those of 2006 and have been  located in a new sub-clade from the Egyptian strains of 2006 and all of the...

Abd Elwanis, N.D.; Abou El Khair, M.A.; Afaf H. Amin; Azab, A. and A.O. Abd El Rahman

...gainst the H5N2 viral antigen after immunizing 3 weeks old chicken with a dose of 0.3 ml/bird, inoculated subcutaneously (s/c) in the nap of the neck, was fairly enough to evoke the required level of immunity. The approach through which this vaccine could be used as a possible potential vaccine candidate for protection of chickens against the highly pathogenic avian influenza (HPAI) virus has been discussed.

...

Ahmed Abd El Samie Hassan Ali

... and detection of its antigens on MDBK cells using outgrowth ELISA and immunofluorescence as well as measurement of BVDV antibodies by serum neutralization test. Virus antigen detection was reported on days 5 & 7 while the antibodies were initially detected on day.

...

Hassanein, S.A.*; Ibrahim, A.K**; Mervat, M.M.* and   Nahed,K.A.*

... DNA (RAPD) analysis. Antigenic comparison using VN test and their effect on MDBK cell culture detected no difference, thus demonstrating the close antigenic relationship between the examined herpesviruses. Using the restriction endonucleases BamHI and EcoRI revealed no difference between the examined five viruses. But by the use of Hindlll restriction endonuclease and RAPD, fingerprint patterns were capable of differentiati...

Eman, Abo Hatab*; Hussein, HA.; El-Sabagh IM. and Saber, MS.

...lates were identified antigenically using VP6 monoclonal antibodies (MAbs) based antigens capture ELISA that able to detect any group A rotavirus. The viral RNA was extracted from the tissue culture harvest of the propagated viruses and RT-PCR using primers specific for VP6 and VP7 of group A rotaviruses was employed and confirmed the molecular characterization Of the isolates viruses with the correct and expected bands. The...

El-Sabagh, I.M.; Hussein, H.A.; Amer, H.M.; El-Sanousi, A.A.; Reda,I.M. and M.A. Shalaby

... major neutralization antigen (VP7) of bovine romvirus Nebraska calf diarrhea virus (NCDV) strain in insect cells. The full-length DNA copies of RNA segment 9 (coding for VP7 protein) of NCDV was inserted into a baculovirus transfer vector under the control of the polyhedrin promotor. A recombinant baculovirus carrying the VP7 gene was constucted through homologous recombination between the baculovirus transfer vector carrying the VP7 gene and Autographa calif...
Eman, M. M. Soliman*; Taha, M. M.*;El-Sanousi A,;Shalaby M. S. Wasscl , Youscf Adel*
...and on the eluted RVF antigen from the tested vaccine, the master seed RVF virus gave positive band at 800bp but the RVF virus after inactivation and eluted antigen from vaccine didn't give any bands.
...

Abou zead, A.A., Ali, A.A.H.*and Abd El Hkeem M.M.

...d suffering mainly from digestive disorders. Mortality rate, as well as the characteristic clinical signs, , post mortem lesions and histopathological changes were recorded. Serosurvey of 80 serum samples revealed 5 (6.25%) positive for the presence of IBDV precipitating antibodies. 3 out 12 pooled bursae (25%) were positive to the presence of IBDV precipitinogen. Trials for virus isolation on the CAM of chicken embryos revealed the isolation of 3 IBD vir...

Sabry Y. M. Mahmoud; Maher H. Hosseny and Mamdouh H. Abdel-Ghaffar

...were tested by direct antigen coatingenzyme linked immunosorbent assay (DAC-ELISA) using antisera against PVY, Potato virus X (PVX) and Potato leaf roll virus (PLRV). The results indicated the occurrence of single and mixed infections of three viruses in potato plants. Survey results indicated highly distribution of PVY infected plants, which its yield was affected strongly. Tubers of potato plants which gave a positive reaction to PVY only were collected at h...

A. A. El-Kholy1, S. Vilcek2 and A. M. Daoud1

...ould likely induce an antigenically appropriate immune response. The present study offers a useful molecular epizootiological tool to group local BVDVs in a herd-specific manner at a geographic region to recognize new virus introduction and thus to improve local vaccines and diagnostic assays for effective control policies.

...

Nahed A. Mohamed l, H. A. Hussein2, Fathia M. Mohamed1 and M. A. Shalaby2

... the presence of BVDV antigen. In a trial for the isolation of the BVDV, 69 buffy coat samples were inoculated in MDBK cells, five selected samples that induced a clear cytopathic effect on inoculated cells were passaged for 7 times and subjected for further antigenic and molecular characterization using virus neutralization test and different formats Of PCR assays. The results revealed that the isolated BVDV field strains (...

H.A. SULTANl, H.A. HUSSEIN2, and F.F. EL-KHAYAT3

...ation test (AGPT) and antigen capture- enzyme linked immunosorbent assay (AC-ELISA). Antigenic typing of IBDV from selected outbreaks were carried out by monoclonal antibodies-based AC-ELISA and the pathogenicity of some representative infectious bursal disease virus (IBDV) field isolates were studied. Of the selected 22 IBDV -positive bursal samples, 59.1% (13/22) were typed as classic IBDVs and 40.9% (9/22) were of variant...

M.R. Abd-El Wahab l, H.A. Hussein2, T.M. Asfourl and M.A. Shalaby2

...ead camel cases using antigen capture ELISA and fluorescent antibody assay. Histopathology examination of different organs revealed changes similar to those reported with BVDV infection. The existence of BVDV in sera collected from camel population. in the area (Al-Ain city. Western area or Abu Dhabi- Emirate, UAE) where camels were found was confirmed. The antigen was confirmed using antige

Ahmed Abd El-Samie H. Ali

...kin biopsies by trypsin digestion. Using immunofluorescence. the percentages of infected cells and MHC antigens expression were evaluated. fibroblast and peripheral blood mononuclear (PBMC) cells were in vitro infected and stained by direct double staining with both human and bovine anti-MHC class I and II antibodies labeled with phycoerythrin (pink) 1:20 and anti-BVDV polyclonal antibodies labeled with fluorescein isothiocy...

S. A. Khalil1; M. El-Sayed2; M. M. El-Fayomy2; H. Y. Hassan3 and A. Zaghawa3

...the detection of BEFV antigen by Immunoperoxidase staining. The aim of the present study was to determine the efficiency of transfer of BEFV antibodies from naturally infected cows to newborn calves through colostrum. According to the level of serum neutralizing antibodies (SNA), the cows were allotted into three groups. In the first group (n=9). the mean SNA in naturally infected cows was 46.2. which gave a mean titer neutralizing antibodies (NA) of 28.4 in t...

M.S. Wassel1; Elham A. El-Ebiary1; Soliman, Y.A.l and El-Sayed, M.M.2

...well as remaining its antigenic and immunogenic power in animals using its protein analysis and Western blotting.

...

Arwa H. El -Naggar, Nirmeen G. Shafiek, and M. S. Wassel.

...nst reference B. Rota antigen using direct and indirect fluorescein assay (FA. IFA). Both assay gave good result as the working dilution of the fluorescein isothiocyanate conjugated B.rota IgG was 1:30. 

...

N. A. Sherif, Lamiaa M. Omar, S. M. Shafei and Elham A. El-Ebiary.

...ully for detection of antigens and antibodies

...

Anak Agung Ayu Sri Trisnadewi*, I Gusti Lanang Oka Cakra

...nsumption, and nutrient digestibility. The results showed that body weight and feed consumption showed no significant differences between all treatments and tended to be the highest in treatment C. Dry matter and crude protein digestibility of treatment A was the lowest but the highest on nitrogen-free extract (NFE) compared to treatments B, C, and D. The crude fiber digestibility was high...

Nguyen Binh Truong1,2*, Ho Xuan Nghiep1,2, Tran Trung Tuan1,2

...n feed intake, nutrient digestibility and nitrogen retention. The feed sources were finely ground and used in the experiment were Maize (Ma), Brocken rice (Br), Cassava chip (Ca) and Wheat (Wh). The proportion of combination (% dry matter intake) in two energy feed sources was 15% and 15% such as MaCa, MaWh, BrCa and BrWh. The results show that dry matter intake per body weight of BrWh (3.42%) and BrCa (3.40%) treatments tended to high compared to the MaCa (3....

Divanildo Outor-Monteiro1,2,3*, José António Silva2,3,4, José Luís Mourão1,2,3, Victor Pinheiro1,2,3

...cages displayed reduced digestive organ development, decreased caecal acetic acid and increased butyric acid concentration, compared to their counterparts. When comparing slaughter ages, the 84-day-old rabbits exhibited higher dressing out percentages, lower relative organ weights (liver, kidney, lung and heart), decreased hind part proportions and lower cooking loss values. Furthermore, the hind legs of these rabbits had a greater proportion of muscle and les...

Rawaa Saladdin Jumaa

...at P4b gene analysis of pigeon poxvirus virus in locally infected pigeons with crust lesions in the eye and nostril cere. The DNA extracted from the collected samples was amplified using conventional PCR by amplification of the P4b gene. The positive samples had been inoculated on chorioallantoic membrane (CAM). Thickening of CAM were seen in the first passage, whereas pock lesion at the second (2nd) and third (3rd) passages...

Firda Dimawarnita1*, Edwina Gabriela Tesalonika Mustamu2, Yora Faramitha1, Haryo Tejo Prakoso1, Wildan Aulia Noorsy1, Komang Gede Wiryawan2

...ation (NH3), dry matter digestibility (DMD), crude fiber digestibility (CFD), organic matter digestibility (OMD), methane gas production, fulvic acid content, and bacterial population. The results indicated that the treatment did not have a significant effect on DMD, OMD, pH, NH3 concentration, methane gas, and bacterial populations. Treatment showed a significant result (P<0.05) on tot...

Nurlan Akhmetsadykov1*, Tanatar Kydyrov2, Moldir Akhmetzhanova1, Gulnazi Akhmetova3, Maxat Berdikulov4 

...uce a recombinant GM6 antigen derived from T. evansi and investigate its immunological properties for the detection and diagnosis of trypanosomosis. To create a recombinant antigen, bioinformatic analysis of the primary structure of T. evansi GM6 antigen from several regions was carried out. The protein sequence was reverse translated to the nucleotide sequence, after which the gene was sy...

Faisal Alzahani1* and Mohammed Abu El-Magd2*

...tudied but the precise epigenetic molecular mechanisms for the combined therapy are still unclear. This study aimed to investigate the epigenetic mechanism of CAPE and/or CNPs on human HepG2 cells. The results revealed a significantly higher cytotoxic effect for CAPE on HepG2 cells than CNPs. The combined therapy with CAPE and CNPs exhibited significantly higher expression of the apoptotic Bax gene and lower expression of th...

Doungnapa Promket1*, Khanitta Pengmeesri1, Jennarong Kammongkun2, Thassawan Somchan1 

...th three hundred Thai indigenous chickens. This study employed PCR-RFLP genotyping of the MTNR1C and NPY genes to investigate their association with egg production traits. The results revealed significant associations between these genes and several egg production parameters on Thai indigenous chicken populations. In the case of the MTNR1C gene, significant effects were observed on WE360d, NE360d, and E/M (P < 0.05). Spec...

Muhammad Wasif Gulzar* and Jawad Hussain

...tion of artificial intelligence (AI) tools in veterinary medicine and infectious diseases (viral, bacterial, and parasitic), in contrast to the heavily regulated field of the development of medical devices (which include AI) in human medicine. When introducing such potent technology that might affect the welfare of both humans and animals, we must acknowledge the significance of exercising prudence and accountability. As veterinary experts, it is therefore our...

Ram Prasad Ghimire

...etermining the apparent digestibility of fodder nutrients of those browse species. Both of the experiments were conducted in Randomized Complete Block Design (RCBD) with six treatments and five replications on native hill goats of Nepal. The fodders of popular five browse species from the pasturelands of Nepali middle hills; Dhayero (Woodfordia fruticosa Kurz), Musure katus (Castanopsis tribuloides (Sm.) Lindl.)), Bamboo (Bamboosa arundinacea (Retz.) Willd.), ...

Siqiang Li1,2, Tiantian Wang1, Peng Sun1, Airong Gao1, Xin Gong1, Yuanhong Xu2, Baogen Wang2, Jun Wu1* and Bo Liu1*

...s, which was capable of digesting α-1,2 linked mannose residues in high selectivity. The highest enzymatic activity of MDS I occurred at a pH of 7.0 and a temperature of 42°C. Enzymatic activity of MDS I was also influenced by metal ions, which were increased to 22% and 17%, respectively, by Co2+ and Cu2+ (2 mmol/L each), while were inhibited to some extent by Ca2+, Mg2+, Mn2+ and Zn2+. This study has laid the foundation for the application of MDS I ...

Limbang Kustiawan Nuswantara, Eko Pangestu, Marry Christiyanto, Santoso Dwi Pratomo, Elyza Zahrotul Muhtaromah

...n of tree legume on the digestibility value and total digestible nutrients (TDN) of dairy goats. This study used dairy goats in the lactation phase were 10 of the Etawa Crossbreed and 6 Sapera. The materials used are feed in the form of tofu dregs, elephant grass and concentrate composed of distillers dried grains with soluble (DDGS), corn gluten feed (CGF), coconut cake, soybean meal, wheat bran, molasses, gamal leaf flour,...

Nguyen Binh Truong1,3*, Nguyen Ba Trung1,3, Nguyen Thi Bich Hạnh2,3

...n feed intake, nutrient digestibility and weight gain. The NDF levels used in the experiment were 43, 47, 51 and 55 (% dry matter intake) corresponding to NDF43, NDF47, NDF51 and NDF55 treatments. Dry matter intake per body weight of NDF43 (2.40%) treatments tended to (P>0.05) high compared to the NDF47 (2.20%), NDF51 (2.22%) and NDF55 (2.16%) treatments. Total metabolism energy inclined to decrease (P>0.05) from NDF43 to NDF55 treatments. It was 42.8, 3...

Joseph Oyiguh Abraham1, Cornelius Arome Omatola1*, Martin-Luther Oseni Okolo1, Ruth Foluke Aminu1, Emmanuel Edegbo1, Olubunmi Marvelous Emurotu1, Danjuma Muhammed2, Jesse Joseph Chock3, Helen Ojomachenwu Ocean4 and Yahaya Ocholi5

...ns in Idah, Kogi State, Nigeria. Sera obtained from 1000 consented individuals were screened for HBsAg, anti-HCV, and HIV antibodies using commercial immunoassay test kits. Relevant demographic information was obtained from each participant with a structured questionnaire. Of the 1000 participants, HBV, HCV, and HIV were detected in 28 (2.8%), 2 (0.2%) and 3 (0.3%), respectively. However, no participant was simultaneously infected with any two or three viruses...

Tran Trung Tuan1,2, Nguyen Binh Truong1,2

...uo; by-product (RDB) on digestibility and nitrogen retention of goats. Four female goats, with an average initial body weight used in this experiment was 27.7±1.63 kg at about 10 months old. The experiment was designed as Latin Square with 4 treatments and 4 replications. The study composed of 4 periods. Each period lasted for 3 weeks with 2 weeks for adaptation, followed by 1 week for data collection of feed intake, faeces, and urine to determine the n...

Phoebe Lyndia Tolentino Llantada1,2*, Midori Umekawa2, Shuichi Karita2

...uctivity. The efficient digestion and utilization of fibrous feed materials are attributed to the foregut, midgut, and hindgut with the aid of the microorganisms that thrive in this region. However, knowledge and studies on bacterial communities across the GIT are very limited. Most researches only focus on the rumen or the hindgut. Therefore, this study was performed to evaluate the bacterial microbiota in three gut regions (foregut: the rumen, reticulum, oma...

Alif Rahman Rohim Puarada*, Safika, Agustin Indrawati

...on birds of paradise, indigenous to Indonesia. Their gastrointestinal microbiome, intricately shaped by a unique ecological milieu, presents a compelling avenue for exploring AMR genes’ reservoir and dissemination mechanisms. Fecal samples were collected from Papua, Indonesia. The DNA was extracted using the DNeasy Power Soil Pro Kit. A shotgun metagenomic sequencing process from the MGI DNBSEQ-G400 platform was carried out to obtain DNA sequence data fr...

Roni Pazla1, Mardiati Zain1*, Fauzia Agustin1, Yetti Marlida1, Zaituni Udin2, Jaswandi2, Masrizal2, Hendri2, Windu Negara3, Totti Tjiptosumirat3, Ezi Masdia Putri3, Multiviza Muslim4

... protein (CP) and total digestible nutrients (TDN) in a ration to enhance the productivity of Pesisir heifers. We evaluated consumption, nutrient digestibility, production performance, and the percentage of first estrous in sixteen heifers at 12-15 m and averaging 100.09 kg in a randomized block design with a 2 x 2 x 4 factorial pattern, where factor A represented crude protein, and factor B represented the TDN level of the ...

Roni Pazla1, Mardiati Zain1*, Fauzia Agustin1, Yetti Marlida1, Zaituni Udin2, Jaswandi2, Masrizal2, Hendri2, Windu Negara3, Totti Tjiptosumirat3, Ezi Masdia Putri3, Multiviza Muslim4

... protein (CP) and total digestible nutrients (TDN) in a ration to enhance the productivity of Pesisir heifers. We evaluated consumption, nutrient digestibility, production performance, and the percentage of first estrous in sixteen heifers at 12-15 m and averaging 100.09 kg in a randomized block design with a 2 x 2 x 4 factorial pattern, where factor A represented crude protein, and factor B represented the TDN level of the ...

Babi Kyi Soe1*, Toe Win Naing2, Su Lai Yee Mon1, Nay Chi Nway3, Hiroshi Sato4

...ecies of cattle, e.g. indigenous, crossbred dairy, and crossbred beef, are raised together on most farms. Anaplasma spp. are obligate intracellular rickettsial vector-borne pathogens that impact on livestock farmers with major economic constraints and eventually threaten human health in cases of zoonotic species involvement. Therefore, our study aimed to identify Anaplasma spp. infection in a bovine host using both microscopy and molecular techniques. A total ...

Ida Indrayani*, Andi Andri, Tevina Edwin

 
 
...are based on dry matter digestible (DM) originating from food crop waste. The sustainability of the beef cattle business was assessed based on four indicators economic, social, environmental, and institutional. The total feed availability of Pesisir Selatan regency was 251,378 Tons DM/year, while the current total feed needs are 78,823.93 Tons/DM/year. The ability of the area to accommodate ruminants based on the total availability of feed in the Pesisir Selat...

Sepri Reski*, Maria Endo Mahata, Montesqrit, Ridho Kurniawan Rusli

...room temperature and refrigerator temperature, while factor B represents the storage time, namely 1, 2, and 3 weeks. The observed variables included the physical characteristics of the eggs (egg weight, shell thickness, shell weight, yolk weight, albumen weight, yolk colour, and Haugh unit). The results revealed no significant interaction (p>0.05) between temperature and storage duration concerning the physical quality (egg weight, shell thickness, shell we...

Ahmed K. Fahd*, Ali H. Saliem

...he analgesic impact of Apigenin and Diclofenac in mice model. In the beginning, 84 male albino mice were selected, acclimated, and categorized into four equal groups: The 1st tested by the hot plate, the 2nd treated with acetic acid, the 3rd examined by application 10 μl of 2% formalin solution and the 4th was examined by the tail-flick test. Within each experiment, the study mice were subdivided equally into three subgroups; the 1st was administered only d...

Eluzia A. Barus*, Khansa N. Salsabilah, Achmad Firman, Linda Herlina

... method at Camara Farm, Cigendel Village, Pamulihan District, Sumedang Regency, in October 2023. Data collection consisted of primary and secondary data obtained using the interview method. Based on the research results, Camara Farm’s average total production cost per period for a year was Rp. 2,342,573,664. The average revenue per production period was Rp. 2,429,151,070, so the average revenue obtained per production period was Rp. 87,170,606 with profi...

Shahid Iqbal1, Arshad Khan, Ali Hazrat1*, Gul Rahim, Mohammad Ihsan1, Umar Zad Gul1, Maryam Bibi1, Khadija Bibi1 and Muhammad Mukhtiar2

...cipal component with an Eigenvalue of above 0.99 accounted for 61.4% of the total variation, where the 1st PC shows a total variation of 19%, the 2nd PC showed 34.87%, 3rd PC had 49.38% while the 4th PC showing a total variation of 61.4%. Based on cluster analysis all the genotypes were divided into 2 main lineages and further subdivided into 6 clusters where the genotypes A1 and A9 were found the most diverse and were found at the extreme of the Dendrogram. F...
Nusrat Bano1, Muhammad Tayyab1*, Bushra Muneer2, Sehrish Firyal1, Abu Saeed Hashmi3, Muhammad Wasim1 and Ali Raza Awan1
...d for dengue specific antigen and antibodies. In this study 20 patients of febrile illness other than Dengue (OFI), were selected as control. The frequency of various clinical features and alterations in hematological and biochemical parameters were assessed for both cases and controls. Total 108 (31.3%) patients were serologically confirmed for dengue infection. Seropositive 108 cases were classified as classical DF 81(75%), DHF 22(20.4%) and DSS 5 (4.6%). Me...

Kunlayaphat Wuthijaree1, Pattaraporn Tatsapong1, Sukanya Yung-Rahang2, Prayad Thirawong2, Koonphol Pongmanee2*

...(worm burden) in Thai indigenous chickens (Gallus gallus domesticus). A total of, 229 chickens (113 males and 116 females) were investigated in 3 selected districts in central Thailand. Chickens were raised under extensive backyard conditions and then slaughtered at ages 12, 14, 16 and 18 weeks. Standard parasitological procedures were used to determine the worm burden in the gastrointestinal tracts. The R software application was used to compute and assess al...

SYED MUJTABA HAIDER1, KHIZAR HAYAT BHATTI1*, EJAZ HUSSAIN SIDDIQI1, SYED MAZHAR IRFAN1,`2, & ALLAH BAKHSH GULSHAN2

...treptococcus pyogenes; Shigella sonnei; Escherichia coli (1) (4C11); Escherichia coli (2) (ATCC 13048) and Neisseria gonorrhoeae). Agar well diffusion method was applied to assess the antibacterial activity of these extracts. Their antibacterial activities were associated with typical chemical like gentamicine (antibiotic) as positive control. This study revealed that S. pyogenes, S. aureus and N. gonorrhoeae had the maximum inhibition to different extracts of...

KAFEEL AHMAD1, NIMRA ARSHAD1, ZAFAR IQBAL KHAN1, KINZA WAJID1, HUMAYUN BASHIR1, IJAZ RASOOL NOORKA2, MAHPARA SHEHZADI3, HAFSA MEMONA4, MADIHA SANA4, KHALID NAWAZ5, MUDASRA MUNIR1, IFRA SALEEM MALIK1, TASAWAR ABBAS6, ABRAR HUSSAIN7, MUHAMMAD SHER8, MUHAMMAD FAHAD ULLAH6, MUHAMMAD AKRAM QAZI9, MUHAMMAD NADEEM10, MUHAMMAD RIZWAN QURESHI10

... followed by their acid digestion. Buffalo blood samples were centrifuged and the blood serum was separated which was then digested using acids. The atomic absorption spectrophotometer was then used to analyze the heavy metals in the digested samples. The results were analyzed statistically. Two-way ANOVA was used to find out variance and correlation in all samples. The nickel concentratio...

ARIFA ZEREEN & ALMAS JAHAN

...ch seriously affected indigenous plant communities. This has caused destruction of local vegetation growing there and exposed land for colonization of invasive and exotic plant species. Besides this many exotic species have been planted as ornamental plants at the green belts. With the passage of time the exotic plant species also called Invasive Alien Species (IAS) is going to be a serious threat to local ecosystems. This research work was planned to determin...

Wesam Jasim Hansh 

... of 28S rRNA gene of F. gigentica from cows were placed in the database of the GenBank with accession numbers (LC731384, LC731385, LC731386, LC731387, LC731388, LC731389, LC731390), and 10 other sequences of F.gigentica from buffaloes (LC730609, LC730653, LC730654, LC730655, LC730656, LC730657, LC730658, LC730659, LC730660, LC730661) were also recorded in this study. In phylogenetic analysis, Iraqi F. gigantica samples from...
SHAHZADA NADEEM ABBAS1, KIRAN JAMSHED1, SARAH BUSHRA NASIR2,
SAMAN SHAHID3 & NASIR MAHMOOD4
... methanolic extracts of Nigella sativa and Ficus carica seeds were used against bacteria (Staphylococcus aureus and Escherichia coli) and Fungi (Candida albicans, Fusarium oxysporium and Microsporum canis) to check their antimicrobial activities. For antibacterial assays, the volume and concentrations of extracts used were 0.5 μl (62.5 μg), 1 μl (125 μg), 5 μl (625 μg) and 10 μl (1250 μg). Specific laboratory protocols of antibacterial ...

SHEIKH AJAZ RASOOL1, MUHAMMAD SALMAN RASOOL2 & MUNAZZA AJAZ3

...neuro-diseases). These epigenetical agents have been a source of concern for our planet regarding food safety issues (e.g. infected meat). No doubt, man has made significant achievements in effective and neo-antimicrobials research, one wonders why not a single infectious agent has been completely knocked out. Our group has been focusing on ascertaining the basis of antibiotic deferring processes acquired by (indigenous) cli...

Olaolu Fadeyi1,2, Oluwatoyin Fabiyi3*, Tesleem Bello4 and Gabriel Olatunji5

...les. Okra production in Nigeria and many parts of the world is threatened by the menace of RKNs and causing significant yield losses in production. Many growers result to the use of synthetic nematicides to combat this risk. However, due to the increasing awareness about the detrimental effects of chemicals on human health and the environment, its continuous use is discouraged worldwide. The search for other eco-friendly alternatives has led to the use of diff...

Bela Putra1*, Budi Prasetya2

...Keywords | Acidic soil, Digestibility, Gamma radiation, Macro mineral, Pennisetum purpureum cv. Mott, Rumen fluids
...

Ho Xuan Nghiep1,2, Nguyen Binh Truong1,2*

...d consumption, nutrient digestibility and weight gain of beef cattle. Four male Charolais crossbred cattle (363±14.1 kg) were used in Latin square design (4x4) on 4 periods (3 weeks/period). The treatment replaced maize with coconut meat waste 0, 25, 50 and 75% (CMW0, CMW25, CMW50 and CMW75, respectively). The result showed that the dry matter (DM) intake tended to decrease (P>0.05) from CMW0 to CMW75. In detail, the DM per body weight was 1.64, 1.65...

Jacob Situmeang1, Lovita Adriani1*, Deny Saefulhadjar1, Safri Ishmayana2

...ity, which can increase digestive tract enzymes in laying hens. In this study, it is hoped that probiotics can increase protein levels and reduce lipid and cholesterol levels in chicken egg yolks. This study aims to determine the activity of the protease and lipase enzymes from yogurt and the effect and level of use of probiotic powder, which can increase protein and reduce lipid and cholesterol levels in chicken egg yolks. The study was conducted using a comp...

Putri Okta Shafura1, Mardiati Zain2*, Elihasridas2, Bima Bagaskara1, Ummi Amanah1, Laras Sukma Sucitra1, Bella Veliana Utami1, Roni Pazla2, Erpomen2, Ezi Masdia Putri3, Rusmana Wijaya Setia Ningrat2, Riris Delima Purba3, Ruslan Abdul Gopar3, Putut Suryo Negoro3

... citronella to increase digestibility and provide optimal supplementation of Saccharomyces cerevisiae and mineral sulfur for rumen fluid characteristics. This study employed a completely randomized design comprising four treatments with four replications, with an incubation time of 48 hours. The ration was prepared based on a 50:50 composition of forage and concentrate, including P0 (Control), P1 (0.5% S. cerevisiae), P2 (0.3% sulfur), and P3 (0.5% S. cerevisi...

Srisukmawati Zainudin1,3, Hartutik2, Edhy Sudjarwo2, Osfar Sjofjan2*

...al meal on the nutrient digestibility of local chicken was assessed, with a focus on its short chain fatty acid and omega content. The initial phase involved conducting a skipjack tuna offal meal experiment using a complete randomized design with 3 treatments and 9 replications to examine the short chain fatty acid and omega content. Following this, a second experiment was conducted using 24 male Kampong Unggul Balitnak (KUB) local chickens, employing a comple...

Waseem Abbas, Imtiaz Ahmed* and Imran Khan 

...ethods. In-vitro starch digestibility and total starch were determined by Megazyme starch assay kit. A nine-point hedonic scale assessed bread samples for consumer acceptance. The findings of proximate composition showed a significant increase in ash at 2 and 3 % incorporation. The fiber content also significantly increased at the incorporation ratio of 1, 2 and 3%. In CWB, fat concentration at 2 and 3% incorporation remained almost equal to that of CB. The pr...

Addisu Jimma1,2*, Aberra Melesse1, Aynalem Haile3, Tesfaye Getachew3  

...ctive in implementing indigenous breed improvement strategies to achieve genetic progress, economic benefits, and livelihood improvements for smallholders in the pioneer sheep breed improvement cooperatives in Ethiopia. Although the Doyogena sheep CBBP is one of the well-performing breeding cooperatives, there is a lack of up-to-date information regarding farmer perspectives on morphological and reproductive changes (such as conformation, coat color, litter si...

Muhammad Shuaib1*, Abdul Hafeez1, Woo Kyun Kim2, Aamir Khan3 and Abubakar Sufyan4

... diet of laying hens on digesta viscosity, fecal consistency, hematology, serum biochemistry, and intestinal morphometrics during peak egg production periods. A total of 160, 28-weeks old golden misri (brown) laying hens were distributed in the following groups. A basal diet of the corn-soybean meal was formulated as a control group and a soybean meal in the basal diet was replaced with 3%SH, 6%SH, and 9% SH respectively. The results of the feces proximate ana...

Javairia Shafi*, Kashifa Naghma Waheed, Zahid Sharif Mirza and Shaista Razaq

... difference in apparent digestibility coefficient (ADC) of dry matter among treatments. Feed ADC (protein) with duckweed at 30% inclusion level was significantly lower (P<0.05) compared to the other treatments. There was no significant difference in muscle composition of fish reared under the four treatments. Results of the present study showed that duckweed can be used to replace up to 20% of fish meal in feed of catla without any negative effect on fish g...

Tanveer Jilani1*, Irfan Khan2, Asmat Salim2, Fareena Bilwani1, Bushra Moiz3 and Mohammad Perwaiz Iqbal1,4

...roid specific surface antigens CD34, CD235a and CD71. The purity of CD34+-derived EPCs was determined to be 95%-98%. Cord blood samples contain immature cells, which can result in contamination of PBMNCs layer. Thus, obtaining EPCs from CD34+ peripheral blood cells of healthy adult humans is superior as the risk of contamination is minimal. The method of isolation and purification of CD34+-derived EPCs used in this study is simpler, user friendly and is more e...
Mouhamed Zakiou Kolawole Adissa Raimi1, Ghulam Hussain2, Nousheen Zafeer3, Faheem Ahmad1, Muhammad Irfan4, Anwar Ullah1, Imdad Kaleem1 and Asghar Shabbir1*
...de effects. Blackseeds (Nigella sativa) and honey possessing potent neuroprotective, antioxidant, and anti-inflammatory properties with no reported side effects can be a prospective candidate for an alternate remedial treatment of MS in animal model as well as in humans. In this study we established a new animal model (quail), to assess the synergistic efficacy of honey and black seed against demyelination within brain. A total of 35 male quails were used, amo...

Syed Makhdoom Hussain*, Hafiza Hina Shafqat, Muhammad Faisal, Mahnoor Saleem, Zeeshan Yousaf and Muhammad Amjad

...current study, the best digestibility results of crude fat (CF) (81.25%), crude protein (CP) (69.39%), and gross energy (GE) (71.74) were seen in VI-level diet and these results were statistically (p<0.05) different from all other test diets. However, the test diet-IV had the least digestibility value of nutrients, including CF (73.55%), CP (49.17%) and GE (65.96%). It was concluded that C. catla fingerlings showed improv...

Shishir Sharma1*, Suk Bahadur Gurung2, Ritesh Kumar Yadav1, Bibek Phulara1 and Laxmi Prasad Joshi1

...nents were significant (eigenvalues > 1) and accounted for 44.60%, 15.30%, and 13.60%, respectively of the total variance. PC1 comprised parameters including percent disease index (PDI), area under the disease progress curve (AUDPC), disease incidence percent (DI%), and infected leaves per plant (IL/P) that were mostly connected to quantitative resistance; PC2 was related to lesion number and lesion breadth; PC3 was mostly concerned with grain yield, while ...

Iqra Munir*, Farrah Iftikhar, Hira Fatima, Sunbal Khalil Chaudhari and Roha Ramash

... to gather the native indigenous knowledge about therapeutic uses of medicinal plants in Mandi Bahauddin, District Gujrat, Punjab, Pakistan. Ethnomedicinal information was collected using surveys and personal interviews with local inhabitants, involving 30 males and 20 females across a wide range of age groups ranging from 25 to 80 years. The participants also included traditional practitioners of herbal medicines, known as ‘tabibs’ and ‘haki...

Umair Ahmad1*, Asad Sultan1, Sarzamin Khan1 and Muhammad Tahir2

... immunity, and nutrient digestibility of broilers fed a high level of poultry by-product meal-based (PBM) diet. A total of 320-day-old broiler birds were assigned to four dietary treatments CON, N-CON, GPP1, and GPP2. CON a commercial corn-soybean meal-based diet as per ROSS-308 nutritional specifications. N-CON was a 6.5% PBM-based diet and to this group, a ginger-derived phyto-protease was added at two levels in treatments GPP1 (50 mg kg-1 feed) and GPP2 (10...

Dio Fico Felsidan Diatmono1, Seraphina Kumala1, Pradita Iustitia Sitaresmi2, Stefani Winda Paramita1, Megawati Andi1, Yustina Yuni Suranindyah3, Diah Tri Widayati1*

...protein (CP), and total digestible nutrient (TDN) intake. The data results were analysed using Independent Sample T-Test and the correlation between blood metabolite profiles and nutrients was analysed using bivariate correlations by Pearson. The data showed that the cholesterol level was higher (p<0.05) on follicular phase (134.04±25.11 mg/dL) than on luteal phase (96.74±22.67 mg/dL), while no differences were found in other parameters despit...

Ndubuisi Chinedu Adikuru1*, Paul Inyang2, Abraham Agwu Ngwuta1, Chinyere Prisca Anyanwu1 and Rosemond Adaohuru Alagba1

...inforest agroecology of Nigeria emanates from altered rainfall pattern caused by climate variability and change. Selected maize (Zea mays) varieties developed for drought tolerance were evaluated over two years to identify those which could be included in the strategy for adapting to climate variability and change in the rainforest agroecology of Nigeria. The treatments were ten (10) maize varieties (eight drought tolerant v...
Abdel Satar Arafa
...ever they have a wide antigenic variability in different lineages.
...

Noor-ul-Ain1, Zulfiqar Ali1*, Saba Naz1, Kiran Saleem1,2, Syeda Rida Hasan1, Sana Arif1, Rida Ahmad1,3, Nida Naeem1, Zarmina Zainab1 and Aliza Batool1,3

...ars (Ursus thibetanus laniger) are natural inhabitants of deciduous forests. They live solitary in their natural habitat. Their captive behaviour was studied by taking two Asiatic black bear pairs as study subjects in Lahore Zoo, Pakistan. The study was based on the provision of environmental enrichments to the bear pairs. Each pair’s pre-enrichment activity budget data was collected by scan sampling method of 70 h, documenting the cage area and behaviou...

Aspen Abutalip*, Batyrbek Aitzhanov, Assiya Mussayeva, Vladislava Suchshikh, Natalya Yegorova

...ed with the flagellar antigen Clostridium chauvoei were used, which sensitised latex microparticles with a size of 0.8-1.1 microns from Thermo Fisher Scientific Corporation. In the process of testing the prepared diagnostic system with vaccine strains of the causative agent of emphysematous carbuncle used for active immunisation of animals in Kazakhstan and field experiments with biological material from sick and deceased animals, results were obtained that in...

Shah Zeb Khan1, Ali Hazrat1*, Farman Ali Khan2, Muhammad Yahya1, Gul Rahim1, Muhammad Mukhtiar3, Muhammad Ajmal Khan4 and Hayat Ullah1

...ungal effect against A. niger and A. flavus with inhibition zones of 19±0.45 and 18±0.39 mm respectively. In case of stem extracts, CS exhibited strongest antibacterial activity against E. coli, S. aurous and S. flexneri with inhibition zone of 24±0.23, 22±0.67 and 20±0.32 mm respectively while the same fraction showed maximum inhibition of linear growth (22±0.76 and 19±0.66 mm) of Candida albicans and A. n

Saira Jabeen1, Muhammad Avais1*, Jawaria Ali Khan1, Kamran Ashraf2, Aftab Ahmad Anjum3

...tion of vaccines, the antigen concentration was adjusted to 10^10 cells/mL, a level determined to trigger highest IHA antibody response in rabbits. Three distinct vaccine types were prepared, each employing different adjuvants. To ensure the quality and safety of these vaccines, quality control assessment was carried out. The efficacy evaluation of the vaccines was performed on 24 locally bred rabbits, divided into 4 groups. Each group received a different typ...

Yan Wang1,2*, Lin Liu3, Na Li4, Yijun Zong1, Wei Liu1, Wenhua Xu1, Qi Wang1, Peijuan Zhang1 and Huiling Feng2,5*

...effects of lymphocyte antigen 6-complex K (LY6K) on aerobic glycolysis and proliferation of lung adenocarcinoma cells. Database analysis confirmed the association between LY6K expression and survival prognosis. Western blot was used to detect the protein expression of proteins. RT-PCR was used to detect the mRNA expression level of genes. Cell proliferation was discovered using the CCK8 test and plate cloning. The impact on the cell cycle was examined by using...
Danish Riaz1,2, Syed Makhdoom Hussain2*, Majid Hussain3, Muhammad Zubair-ul-Hassan Arsalan4 and Eman Naeem2
...erformance, nutritional digestibility, and hematological indicators. Six test diets and one control diet (0 g/kg) were formulated before the beginning of the experiment, with varying amounts of probiotics (0, 0.5, 1, 1.5, 2, 2.5 and 3 g/kg) added to the basal diet. Fish growth performance, nutritional bioavailability, and hematological indices all improved significantly (p<0.05) after being supplemented with probiotics. Fish fed a diet supplemented with pro...

Akbar Khan Khajjak1,4*, Adnan Nazir2, Tehmina Mangan1 and Ali Raza1

... dietary foods of the indigenous population in comparison to other grains, fish, chicken, and meat. A total of 300 farmers were interviewed, therefore 150 farmers from each district Naushehro Feroze and Benazirabad were recorded. In this study, the Cobb-Douglas stochastic production frontier approach was employed for examining the technical efficiency and inefficiency factors in wheat production. The results indicated that the improvement in wheat production i...

Felix O. Takim*1, Gideon Z. Nayan2, Oluwafemi O. Osatuyi1and Israel O. Olayiwola1

...thern Guinea savanna of Nigeria. The experiment was laid as a randomized complete block design with a split-plot arrangement and 3 replications in 2018 and 2019 growing seasons. The main plots were planting time (May, June and July) while the sub-plots consisted of 3 maize-sweet potato intercropping populations(maize at 53,333 plants/ha + 33,333 plants/ha of sweet potato, maize at 53,333 plants/ha + 66,666 plants/ha of sweet potato and maize at 53,333 plants/h...

Amir Ehsan1*, Muhammad Ehsan Safdar1, Amjed Ali1

... Echinochloa colona and Digera arvensis laid out in randomized complete block design. Augmented densities of E. colona (0, 22, 44, 66 and 88 weed plants m-2) enhanced its plant dry biomass up to 348 and 353%; and relative competitive index maximally to 80 and 77% in years 2015 and 2016, respectively. While the corresponding increases in plant dry weight and relative competitive index of D. arvensis were 367 and 360% and 79 and 82%. The enhancement in N (up to ...
Naeema Khatoon Khaskheli1 , Muzafar Hussain Sirohi11, 2,*, Ameer Ahmed
Mirbahar1, Abdul Razak Mahar1, Mumtaz Ali Saand1, Mirza Hussain3
...ies
include Digera muricata (Amaranthaceae), Trianthema portulacastrum (Aizoaceae), and
Corchorus olitorius (Malvaceae). The weed community was dominated by annual herbs. The
community composition among the study sites was highly comparable. The study found a
lack of weed management practices across the sites.
...

Yuping Liu, Sige Wang and Tianyan Yang*

...d considered to be an indigenous fish endemic to Xinjiang. In this paper, the complete mitochondrial genome (16607 bp) of A.cernua was sequenced by high-throughput sequencing technology, including 13 protein-coding genes (PCGs), 22 tRNA genes, 2 rRNA genes, a control region (CR) and a L-strand replication origin (OL), and its gene composition and order were similar to those of most teleosts. The A + T content of the whole mtDNA was 55.39 %, suggesting an obvio...
Ali Bouzekri1,2*, Souheila Slimani1,2, Meryem Nassar1,2, Souad Zaaboub3 and Nora Sakhraoui1
... in adult male domestic pigeons (Columba livia domestica). For ten consecutive weeks and under an artificial photoperiod (19L: 5D), thirty male pigeons weighing approximately 309,20 ± 14,41g were divided equally into six groups as follows: CT served as the control, SPT group orally given with 15 mg/kg BW/day, TT100 and TT50 groups orally administrated with 100 and 50 mg/kg BW/day of TT respectively, and SPT+ TT100 and...

Suparada Saphaphan, K. Teepalak Rangubhet, Phongthorn Kongmun*

...ly, in vitro dry matter digestibility (IVDMD) at 24 and 48 h was highest in the 3.5% UTRS treatment (P<0.001), while that in the MSGBTRS treatment was comparable to PH. The concentrations of ammonia nitrogen (NH3-N) and total volatile fatty acids (VFA) showed no significant differences across the treatments. The populations of total ruminal bacteria and cellulolytic bacteria did not show significant differences among treatments. In conclusion, the utilizati...

Siti Zubaidah1, Chusnul Hanim2, Bambang Ariyadi3, Aji Praba Baskara2*, Zuprizal2

...ell wall after in vitro digestibility was determined by scanning electron microscopy (SEM). The PKC with shell contained the following: 4,417.81 Kcal/kg gross energy, 95.45% DM, 4.51% ash, 15.95% CP, 8.36% EE, 21.21% CF, 49.78% NFE, 0.55% lysine, 0.09% methionine, and 47% lauric acid (LA). The PKC without shell had the following: gross energy of 4,555.43 Kcal/kg, 97.84% DM, 4.26% ash, 22.33% CP, 9.29% EE, 15.43% CF, 50.29% NFE, 0.62% lysine, 0.14% methionine, ...

Advances in Animal and Veterinary Sciences

May

Vol. 12, Iss. 5, pp. 802-993

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